A kind of sample adding device
Technical field
The present invention relates to Chemical Decomposition field, relate in particular to a kind of normal pressure, low pressure chromatographic column sample adding device.
Background technology
At biochemical field, liquid chromatogram isolation technics is the most common technique of compound separation purifying, the low pressure that wherein basic separation method is conventional, normal pressure column chromatography method, usually inhomogeneous because the problem of application of sample causes post bed fillers to be washed, chromatographic isolation weak effect.Even if veteran experimenter, use dropper to drip to be also difficult to guarantee post bed fillers is not washed and all the time smooth evenly.
Summary of the invention
Defect in view of above-mentioned prior art exists, the object of the invention is to propose a kind of sample adding device, utilizes principle of hydrodynamics, reduces the inhomogeneity impact of application of sample link coupled columns bed fillers.
Object of the present invention will be achieved by the following technical programs:
A kind of sample adding device, cup-shaped, comprise perisporium and base plate, described perisporium is provided with a contact-making surface corresponding with chromatographic column standard frosted mouth, described contact-making surface outer surface is provided with at least one and runs through surperficial decompression groove, is evenly provided with at least two guiding gutters, and described base plate is positioned at contact-making surface middle and lower part, described base plate is evenly provided with at least two pod apertures around, and described pod apertures is communicated with described guiding gutter.When sample solution adds fashionablely, sample can slowly flow to post bed along chromatographic column interface and post jamb, and decompression groove communicates with the external world, balance chromatographic column inner and outer air pressure.
Outstanding effect of the present invention is:
Utilize fluid dynamic principle, make application of sample more even, reduced washing away of coupled columns bed fillers, reduced inhomogeneity impact, improved separating effect.
Below just accompanying drawing in conjunction with the embodiments, is described in further detail the specific embodiment of the present invention, so that technical solution of the present invention is easier to understand, grasp.
Accompanying drawing explanation
Fig. 1 is the structural representation of sample adding device.
The specific embodiment
Embodiment:
A kind of sample adding device of the present embodiment, as shown in Figure 1, cup-shaped, comprise perisporium 1 and base plate 2, described perisporium 1 is provided with a contact-making surface 3 corresponding with chromatographic column standard frosted mouth, and contact-making surface 3 outer surfaces are provided with at least one and run through surperficial decompression groove 4, evenly be provided with at least two guiding gutters 5, base plate 2 is positioned at contact-making surface 3 middle and lower parts, and base plate is evenly provided with at least two pod apertures 6 around, and pod apertures 6 is communicated with guiding gutter 5.When sample solution adds fashionablely, sample can slowly flow to post bed along chromatographic column interface and post jamb, and decompression groove 4 communicates with the external world, balance chromatographic column inner and outer air pressure.
The present embodiment utilizes fluid dynamic principle, makes application of sample more even, has reduced washing away of coupled columns bed fillers, has reduced inhomogeneity impact, has improved separating effect.
The present invention still has numerous embodiments, and all employing equivalents or equivalent transformation and all technical schemes of forming, within all dropping on protection scope of the present invention.