CN103734338A - Compounded donkey milk powder for improving immunity - Google Patents

Abstract

The invention discloses compounded donkey milk powder for improving immunity. According to the formula, donkey milk powder is used as a main raw material, Chinese yam, lily, walnuts and lobed kudzuvine root, which can be eaten as both medicines and food, are added, and prebiotics with fructo-oligose and galacto-oligosaccharide, and two probiotics, namely, lactobacillus rhamnosus and lactobacillus plantarum, are additionally supplemented. By virtue of the compounded donkey milk powder, the immunity can be remarkably improved, specifically, the conversion capability of lymphocyte (cellular immune function) is remarkably improved, the production of serum hemolysin (humoral immunity function) is increased, and the carbon clearance capability (mononuclear macrophage function) and the killing activity of NK (Natural Killer) cells (NK cell activity) are enhanced.

Description

A Formula Donkey Milk Powder for Improving Immunity

technical field

The invention belongs to the field of health care, in particular to a formula donkey milk powder for enhancing body immunity.

Background technique

The nutritional content of donkey milk is comprehensive and is the closest to that of human milk. The amino acids in donkey milk protein are complete in variety and in sufficient quantity, including lysine, tryptophan, phenylalanine, methionine (methionine), threonine, isoleucine, leucine, valine Nine kinds of essential amino acids (histidine is an essential amino acid for infants) such as , histidine account for 40.8% of the total amino acids, which is similar to that of human milk (43%). Fatty acids are the main components of milk fat, of which linoleic acid and alpha-linolenic acid are essential fatty acids (EFA). The lack of essential fatty acids (EFA) in the human body will slow down the body's metabolism, resulting in slow growth, reproductive disorders and other diseases. Donkey milk is rich in EFA, the content is about 40 mg/100 g, especially linoleic acid, which is 2.5 times (15 mg/100 g) the content of linoleic acid in cow milk. Donkey milk not only contains a variety of macro elements, but also rich in trace elements. Among the major elements, Ca is rich in content, followed by Na and Mg; among the trace elements, the content of selenium is higher, which is 5.2 times that of milk. The small difference between donkey milk and human milk composition is relatively easy to adjust through ingredients. However, the current research on donkey milk mainly focuses on the analysis of its nutritional components, but there is a lack of in-depth and systematic research on the comprehensive development and utilization of donkey milk. Therefore, it has important social and economic benefits to carry out basic research on the application of donkey milk powder compound formula and broaden its application channels.

Contents of the invention

The present invention provides following technical routes:

The present invention aims to provide a new approach for the market development of donkey milk, using donkey milk powder as the base, adding yam, lily, walnut and kudzu root that can be used for medicine and food, and supplemented with prebiotics containing fructo-oligosaccharides and galacto-oligosaccharides Yuan and Lactobacillus rhamnosus and Lactobacillus plantarum are two probiotics. The present invention includes following technical solutions:

A formula donkey milk powder for improving immunity is prepared according to the following steps:

(1) Use fresh yams with round shape, smooth surface, and no pests and mildew as raw materials for preparation; first wash off the soil on the surface of yams, remove the skin of yams with a scraper and rinse with water; use a stainless steel knife to peel the yams Cut the yam into thin slices of about 1 cm, and soak them in clean water quickly. The slices should be fast, so as not to cause browning after being exposed to the air for too long; ±2) Color protection at ℃ for about 10 minutes can not only get a good color protection effect, but also make the raw materials basically mature to obtain a strong yam flavor; rinse with water immediately after the color protection is completed to remove the adhering citric acid; Add 3 times the quality of water to the tablet, and crush it into a slurry with a pulverizer; add yam pulp to donkey milk at a dose of 3.6 mg/g; that is, add 3.6 mg of yam pulp to every g of donkey milk;

(2) Take fresh lilies, choose large, neat, white and plump lilies, and remove mildew and rotten lilies; remove the peripheral withered and old scales and stem chassis, and clean them with running water; take the cleaned lilies, add 5 times the water, Add 0.4 g of isoVC-Na and 0.1% citric acid to 100 g of lily, heat to 100°C for 5-10 minutes, until the lily is crystal clear, cool immediately, crush and beat; treat the lily pulp twice with a colloid mill , until there are no coarse lily particles; adjust the pH value of the lily pulp to 6.0, add high-temperature resistant α-amylase at a dosage of 200 U/g, and liquefy at 95°C for 25 min; adjust the pH to 5.5, add glucoamylase at a dosage 100-300 U/g, treated at 60-70°C for 1-3 h; enzymatically treated lily hydrolyzate, kept at 100°C for 5 min for enzyme-inactivating treatment; enzymatically inactivated lily pulp in 7.2 mg /g added to donkey milk;

(3) Select fresh walnuts with plump flesh, no damage, no moths, and no mildew to remove impurities such as broken shells and diaphragms, and then rinse the walnuts with clean water; boil the walnuts with 0.2% NaOH for 3 minutes and neutralize them with weak acid , and then washed with water; the walnut kernels with the seed coat removed are crushed in a high-speed tissue grinder, the ratio of solid to liquid is 1:4, and then finely ground with a colloid mill; the distance between the colloid mill is appropriate, generally 5-8 μm; Add the ground walnut powder to donkey milk at a dose of 3.0 mg/g;

(4) Weigh about 2 g of processed kudzu root powder, pour it into the extraction and reflux device, add 60 mL of ethanol with a concentration of 50%, and the solid-to-liquid ratio is 1:30, and reflux in a water bath at 90 °C. Extracted for 3 hours, suction filtered and collected the filtrate, and the clear liquid obtained by suction filtration was transferred to a brown bottle for storage; puerarin flavonoids were added to donkey milk at a dose of 0.24 mg/g;

(5) Chicory fructooligosaccharides were added to donkey milk powder at a dose of 0.985 mg/g;

(6) Preparation of probiotic freeze-dried live bacteria powder: firstly prepare skimmed milk emulsion with a concentration of 10% by mass, divide it into four 500 mL triangular flasks, 200 mL per bottle, and sterilize at 121°C for 20 min Cool to 37°C, inoculate Lactobacillus rhamnosus and Lactobacillus plantarum at a volume ratio of 2%, anaerobically culture at 37°C for 24-28 hours, detect the number of viable bacteria in the culture solution of the two probiotics, and the number of viable bacteria in each culture solution >10 ⁹ CFU/mL, stop the culture, if the number of viable bacteria is <10 ⁹ CFU/mL, continue the culture until it reaches 10 ⁹ CFU/mL; put the culture solution into a glass ampoule under sterile conditions, the liquid level 0.8 cm to 1 cm, capped and placed in a -30°C freezer for quick freezing. After freezing, put the glass ampoule on a tray and put it in a freeze dryer for freeze-drying to prepare powdered freeze-dried strains; Lactobacillus rhamnosus Freeze-dried bacterial powder was added to donkey milk at a dose of 0.4 mg/g, and Lactobacillus plantarum was added to donkey milk at a dose of 0.2 mg/g;

(7) Homogenize the donkey milk mixture added with the above materials. The homogenization pressure and temperature are 20-25 MPa and 45-50°C respectively; the homogenized donkey milk mixture is spray-dried; the spray-drying conditions are controlled It is: the inlet air temperature is 130-160°C, preferably 150°C, the tower temperature is 75°C, and the exhaust air temperature is 70-75°C.

In order to evaluate the invention based on the donkey milk powder to further enhance the immunity of the body, immunocompromised mice were gavaged in groups, including a normal saline group, a donkey milk powder group and a formula donkey milk powder group.

After the immunocompromised mice were prepared by intraperitoneal injection of cyclophosphamide, they were administered by intragastric administration for 28 consecutive days according to the intragastric administration group. After the end of gavage, the spleen of the mice was taken, and splenic lymphocytes were obtained by grinding, the lymphocytes were stimulated by adding ConA, and the transformation ability of the lymphocytes in mice of different gavage groups was observed. After the end of gavage, ink was injected through the tail vein to evaluate the carbon clearance ability of mice in different gavage groups; on the fourth day before the end of gavage, Mianyang red blood cells were injected intraperitoneally, and the spleen of mice was taken after gavage to grind Spleen lymphocytes were obtained to evaluate the killing ability of NK cells on the target cell YAC-1; on the fourth day before the end of gavage, Mianyang red blood cells were injected intraperitoneally, and blood was collected after the end of gavage to measure the production of serum hemolysin.

Beneficial effects of the present invention:

1. The formula donkey milk powder of the present invention has the effect of improving immunity;

2. The formula donkey milk powder of the present invention has a fresh taste and a good mouthfeel, is more easily accepted by ordinary people, and is easy to popularize.

Detailed ways

Example 1

A formula donkey milk powder for improving immunity is prepared according to the following steps:

(1) Use fresh yams with round shape, smooth surface, and no pests and mildew as raw materials for preparation; first wash off the soil on the surface of yams, remove the skin of yams with a scraper and rinse with water; use a stainless steel knife to peel the yams Cut the yam into thin slices of about 1 cm, and soak them in clean water quickly. The slices should be fast, so as not to cause browning after being exposed to the air for too long; ±2) Color protection at ℃ for about 10 minutes can not only get a good color protection effect, but also make the raw materials basically mature to obtain a strong yam flavor; rinse with water immediately after the color protection is completed to remove the adhering citric acid; Add 3 times the quality of water to the tablet, and crush it into a slurry with a pulverizer; add yam pulp to donkey milk at a dose of 3.6 mg/g; that is, add 3.6 mg of yam pulp to every g of donkey milk;

(2) Take fresh lilies, choose large, neat, white and plump lilies, and remove mildew and rotten lilies; remove the peripheral withered and old scales and stem chassis, and clean them with running water; take the cleaned lilies, add 5 times the water, Add 0.4 g of isoVC-Na and 0.1% citric acid to 100 g of lily, heat to 100°C for 5-10 minutes, until the lily is crystal clear, cool immediately, crush and beat; treat the lily pulp twice with a colloid mill , until there are no coarse lily particles; adjust the pH value of the lily pulp to 6.0, add high-temperature resistant α-amylase at a dosage of 200 U/g, and liquefy at 95°C for 25 min; adjust the pH to 5.5, add glucoamylase at a dosage 100-300 U/g, treated at 60-70°C for 1-3 h; enzymatically treated lily hydrolyzate, kept at 100°C for 5 min for enzyme-inactivating treatment; enzymatically inactivated lily pulp in 7.2 mg /g added to donkey milk;

(3) Select fresh walnuts with plump flesh, no damage, no moths, and no mildew to remove impurities such as broken shells and diaphragms, and then rinse the walnuts with clean water; boil the walnuts with 0.2% NaOH for 3 minutes and neutralize them with weak acid , and then washed with water; the walnut kernels with the seed coat removed are crushed in a high-speed tissue grinder, the ratio of material to liquid is 1:4, and then finely ground with a colloid mill; the distance between the colloid mill is appropriate, generally 5-8 μm; Add the ground walnut powder to donkey milk at a dose of 3.0 mg/g;

(4) Weigh about 2 g of processed kudzu root powder, pour it into the extraction and reflux device, add 60 mL of ethanol with a concentration of 50%, and the solid-to-liquid ratio is 1:30, and reflux in a water bath at 90 °C. Extract for 3 h, filter with suction and collect the filtrate, transfer the clarified liquid obtained by suction filtration into a brown bottle for storage; Pueraria flavonoids are added to donkey milk at a dose of 0.24 mg/g;

(5) Chicory fructooligosaccharides were added to donkey milk powder at a dose of 0.985 mg/g;

(6) Preparation of probiotic freeze-dried live bacteria powder: firstly prepare skimmed milk emulsion with a concentration of 10% by mass, divide it into four 500 mL triangular flasks, 200 mL per bottle, and sterilize at 121°C for 20 min Cool to 37°C, inoculate Lactobacillus rhamnosus and Lactobacillus plantarum at a volume ratio of 2%, anaerobically culture at 37°C for 24-28 hours, detect the number of viable bacteria in the culture solution of the two probiotics, and the number of viable bacteria in each culture solution >10 ⁹ CFU/mL, stop the culture, if the number of viable bacteria is <10 ⁹ CFU/mL, continue the culture until it reaches 10 ⁹ CFU/mL; put the culture solution into a glass ampoule under sterile conditions, the liquid level 0.8 cm to 1 cm, capped and placed in a -30°C freezer for quick freezing. After freezing, put the glass ampoule on a tray and put it in a freeze dryer for freeze-drying to prepare powdered freeze-dried strains; Lactobacillus rhamnosus Freeze-dried bacterial powder was added to donkey milk at a dose of 0.4 mg/g, and Lactobacillus plantarum was added to donkey milk at a dose of 0.2 mg/g;

(7) Homogenize the donkey milk mixture added with the above materials. The homogenization pressure and temperature are 20-25 MPa and 45-50°C respectively; the homogenized donkey milk mixture is spray-dried; the spray-drying conditions are controlled For: the inlet air temperature is 130-160°C, the tower temperature is 75°C, and the exhaust air temperature is 70-75°C.

Embodiment 2 immune test

Preparation of immunocompromised mice: 72 BALB/c mice were purchased, placed in the animal room for observation for one week, and injected intraperitoneally with cyclophosphamide solution every other day, at a dose of 100 mg/kg on the first day and 80 mg/kg on the third day Dose injection to prepare immunocompromised mice.

The setting of the mice gavage group: the mice were randomly divided into 3 groups, 10 in each group, followed by the blank group, the donkey milk group, and the formula donkey milk powder group. The period of gavage for mice in each group was 28 days, and the gavage volume of each mouse was 300 μL/day. The mice in the blank group were fed with 0.85% sterile normal saline, and the mice in the donkey milk powder group and the formula donkey milk powder group (the formula donkey milk powder prepared in Example 1) were gavaged with a dose of 5 g/kg.

Preparation of splenocyte suspension: Mice were killed by vertebral dislocation, soaked in 75% ethanol for 2 min, and disinfected. Place the mouse in the dissection tray, dissect the mouse along the peritoneum, take the spleen aseptically, place it in a plate filled with an appropriate amount of sterile Hank's solution, remove the adherent connective tissue on the spleen, wash it gently, and transfer the spleen to a sterile Bacteria Hank's liquid plate. Gently grind the spleen on 40-mesh gauze with tweezers, and filter through a 200-mesh sieve to obtain a single-cell suspension. After the cell suspension was centrifuged at 1000 r/min for 10 min, the erythrocyte lysate was added at a ratio of 10:1, and mixed gently by pipetting. The lysis time was about 5 min. Centrifuge at 1000 r/min for 10 min, remove the supernatant, and wash the cell pellet twice with sterile Hank's solution. Suspend the cells in 1 mL RPMI1640 complete culture medium, count the number of living cells (should be above 95%) by staining with trypan blue, and adjust the cell concentration to 3 × 10 ⁶ cells/mL.

Determination of cellular immune function - mouse lymphocyte transformation experiment: divide each splenocyte suspension into 4 wells into a 24-well cell culture plate, 1 mL per well, and 3 wells are used as parallel wells, and 50 μL of ConA solution (equivalent to 5 μg/mL), add 50 μL of cell culture medium instead of ConA solution to the remaining 1 well as a blank well. Place in a 5% CO ₂ , 37°C incubator for 72 h; 4 h before the end of the culture, gently suck 0.7 mL of the supernatant from each well, then add 0.7 mL of serum-free RPMI1640 culture solution, and add 50 μL MTT solution (5 mg/mL), continue to incubate for 4 h. After the incubation, add 1 mL of acidic isopropanol to each well, and mix well by pipetting to completely dissolve the purple crystals. Then it was divided into 96-well culture plates, and each well was made into three parallel wells, and the absorbance OD value of each well was measured and recorded at a wavelength of 570 nm on a microplate reader. The experimental results are shown in Table 1. The results showed that compared with the normal saline group, administration of a single component of donkey milk powder could not increase the transformation rate of spleen lymphocytes (p>0.05), while the mice in the group of mice administered with formula donkey milk powder The transformation rate of spleen lymphocytes was significantly higher than that of mice in donkey milk powder group (p<0.05).

Table 1 Effects of different gavage groups on the transformation of spleen lymphocytes induced by Con-A (X±SD)

Note: a means p<0.05 compared with blank control group, b means p<0.05 compared with donkey milk powder group.

Measurement of humoral immune function—serum hemolysin test: On the 28th day of gavage, the mice were intraperitoneally injected with 0.2 mL of 2% SRBC, and blood was collected from the orbit on the 5th day after immunization. The collected blood samples were left standing at 37°C for 1 h, then refrigerated and centrifuged at 2000 r/min at 4°C for 10 min, and the serum was carefully drawn to avoid inhalation of red blood cells. Serum was diluted 200 times with SA buffer for determination. Add 1 mL of diluted mouse serum, 0.5 mL of SRBC (10%), and 1 mL of diluted complement to the sample reaction tube in turn; replace the serum sample with 1 mL of SA buffer in the blank control tube, mix well and then keep the temperature at 37 °C Incubate in a water bath for 30 min, then transfer to an ice bath to terminate the reaction. Centrifuge at 2000 r/min for 10 min, take 1 mL supernatant and add 3 mL Douglas reagent, shake well and let stand for 10 min. Use a blank control tube as a blank, and read the absorbance colorimetrically at a wavelength of 540 nm. Determination of the half hemolysis value of SRBC: Add 0.25 mL SRBC (5%) to 4 mL, and read the absorbance colorimetrically at a wavelength of 540 nm, which is the absorbance value at the time of half hemolysis of the SRBC used in the experiment. The half hemolysis value CH ₅₀ of the sample reaction tube was calculated according to the following formula: CH ₅₀ of serum of each mouse = absorbance value of the sample/absorbance value of SRBC half hemolysis × dilution factor. The experimental results are shown in Table 2. The results showed that: in terms of serum hemolysin production in mice, compared with the normal saline blank group, there were significant differences (p<0.05) between the single donkey milk powder group and the formula donkey milk powder group (p<0.05), but the formula The donkey milk powder group significantly increased the production of serum hemolysin than the donkey milk powder alone group.

Table 2 Effects of different gavage groups on serum hemolysin in mice (X±SD)

Note: a means p<0.05 compared with blank control group, b means p<0.05 compared with donkey milk powder group.

Determination of the phagocytic ability of monocyte-macrophages-mouse carbon clearance experiment: Ink injection: Inject ink from the tail vein of mice at a dose of 0.1 mL/kg, and time it immediately after the ink injection. Two minutes and eight minutes after the injection of ink, 20 μL of blood was collected from the inner canthus venous plexus, and immediately added to 2 mL of 0.1% Na ₂ CO ₃ solution, and mixed well. The optical density value OD value was measured at a wavelength of 600 nm, and the Na ₂ CO ₃ solution was used as a blank control. The mice were killed, and the liver and spleen were taken out, and the blood stains on the surface of the organs were blotted with filter paper, and the weights were recorded respectively. The phagocytosis index a was used to represent the carbon clearance ability of mice, and the phagocytosis index a was calculated according to the following formula:

Phagocytosis index a=

The experimental results are shown in Table 3. The results showed that the formula donkey milk powder group could significantly enhance the carbon clearance ability of mice (p<0.05).

Table 3 Effects of different gavage groups on carbon clearance ability of mice (X±SD)

group dose Number of animals/only Phagocytosis index/k corrected phagocytosis index/a Blank control group 0.85% normal saline 10 0.019±0.003 5.008±0.538 Single donkey milk powder group 5 g/kg 10 0.026±0.010 5.716±0.643 Formula donkey milk powder group 5 g/kg 10 0.037±0.012 6.817± ^0.568ab

Note: a means p<0.05 compared with blank control group, b means p<0.05 compared with donkey milk powder group.

Determination of NK cell activity - Lactate dehydrogenase (LDH) assay: Add effector cells (2 × 10 ⁷ /mL) and target cells (4 × 10 ⁵ / mL) each 100 μL (effect-to-target ratio = 50:1), add 100 μL each of target cells and culture medium to the natural release well of target cells, add 100 μL each of target cells and 2.5% Triton to the maximum release well of target cells, and add 100 μL each of the above items Three parallel holes are provided. Incubate for 4 h at 37°C in a 5% CO ₂ incubator; centrifuge the 96-well culture plate at 1500 r/min for 10 min, absorb 100 μL of supernatant from each well, and add 100 μL of LDH matrix solution at the same time, react for 10 min and then Add 30 μL of HCl (1 M) to the wells, measure and record the OD value at 490 nm on a microplate reader. The experimental results are shown in Table 4. The results showed that the formula donkey milk powder group had a significant enhancement effect on the killing activity of mouse NK cells (p<0.05), while the single-component donkey milk powder could improve the killing activity, but the effect was not Not significant (p>0.05).

Table 4 Effects of different gavage groups on the killing activity of NK cells in mice (X±SD)

group dose Number of animals/only NK cell activity/% Blank control group 0.85% normal saline 10 18.813±2.288 Donkey milk powder group 5 g/kg 10 23.673±4.789 Formula donkey milk powder group 5 g/kg 10 31.544± ^4.098ab

Note: a means p<0.05 compared with blank control group, b means p<0.05 compared with donkey milk powder group.

Claims (2)

1. a formula donkey milk powder for improving immunity, characterized in that it is prepared according to the following steps: (1) Use fresh yams with round shape, smooth surface, and no pests and mildew as raw materials for preparation; first wash off the soil on the surface of yams, remove the skin of yams with a scraper and rinse with water; use a stainless steel knife to peel the yams The Chinese yam was cut into thin slices of about 1 cm, and soaked in clean water quickly; the yam slices were protected in 0.2 g/100 mL citric acid color protection solution at (98±2)°C for about 10 minutes; immediately after the color protection was completed. Rinse with clean water to remove the adhering citric acid; add 3 times the quality of water to the yam tablets, and crush them into a slurry with a pulverizer; add the yam slurry to the donkey milk at a dose of 3.6 mg/g; that is, every g of donkey milk Add 3.6 mg yam pulp; (2) Take fresh lilies, choose large, neat, white and plump lilies, and remove mildew and rotten lilies; remove the peripheral withered and old scales and stem chassis, and clean them with running water; take the cleaned lilies, add 5 times the water, Add 0.4 g of isoVC-Na and 0.1% citric acid to 100 g of lily, heat to 100°C for 5-10 minutes, until the lily is crystal clear, cool immediately, crush and beat; treat the lily pulp twice with a colloid mill , until there are no coarse lily particles; adjust the pH value of the lily pulp to 6.0, add high-temperature resistant α-amylase at a dosage of 200 U/g, and liquefy at 95°C for 25 min; adjust the pH to 5.5, add glucoamylase at a dosage 100-300 U/g, treated at 60-70°C for 1-3 h; enzymatically treated lily hydrolyzate, kept at 100°C for 5 min for enzyme-inactivating treatment; enzymatically inactivated lily pulp in 7.2 mg /g added to donkey milk; (3) Select fresh walnuts with plump flesh, no damage, no moths, and no mildew to remove impurities such as broken shells and diaphragms, and then rinse the walnuts with clean water; boil the walnuts with 0.2% NaOH for 3 minutes and then neutralize them with weak acid and, then rinse with water; the walnut kernels with the seed coat removed are mashed in a high-speed tissue masher, the ratio of material to liquid is 1:4, and then finely ground with a colloid mill; the distance between the colloid mill is selected to be 5-8 μm; The walnut powder was added to donkey milk at a dose of 3.0 mg/g; (4) Weigh about 2 g of processed kudzu root powder, pour it into the extraction and reflux device, add 60 mL of ethanol with a concentration of 50%, and the solid-to-liquid ratio is 1:30, and reflux in a water bath at 90 °C. Extracted for 3 hours, suction filtered and collected the filtrate, and the clear liquid obtained by suction filtration was transferred to a brown bottle for storage; puerarin flavonoids were added to donkey milk at a dose of 0.24 mg/g; (5) Chicory fructooligosaccharides were added to donkey milk powder at a dose of 0.985 mg/g; (6) Preparation of probiotic freeze-dried live bacteria powder: firstly prepare skimmed milk emulsion with a concentration of 10% by mass, divide it into four 500 mL triangular flasks, 200 mL per bottle, and sterilize at 121°C for 20 min Cool to 37°C, inoculate Lactobacillus rhamnosus and Lactobacillus plantarum at a volume ratio of 2%, anaerobically culture at 37°C for 24-28 hours, detect the number of viable bacteria in the culture solution of the two probiotics, and the number of viable bacteria in each culture solution >10 ⁹ CFU/mL, stop the culture, if the number of viable bacteria is <10 ⁹ CFU/mL, continue the culture until it reaches 10 ⁹ CFU/mL; put the culture solution into a glass ampoule under sterile conditions, the liquid level 0.8 cm to 1 cm, capped and placed in a -30°C freezer for quick freezing. After freezing, put the glass ampoule on a tray and put it in a freeze dryer for freeze-drying to prepare powdered freeze-dried strains; Lactobacillus rhamnosus Freeze-dried bacterial powder was added to donkey milk at a dose of 0.4 mg/g, and Lactobacillus plantarum was added to donkey milk at a dose of 0.2 mg/g; (7) Homogenize the donkey milk mixture added with the above materials, the homogenization pressure and temperature are 20-25 MPa and 45-50°C respectively; spray dry the homogenized donkey milk mixture. 2. The formula donkey milk powder according to claim 1, characterized in that step (7) spray drying conditions are controlled as follows: the inlet air temperature is 130-160°C, preferably 150°C, the tower temperature is 75°C, and the exhaust air temperature is 70-75°C.