CN103725617A - Aurantiochytrium sp. rich in glyceride type DHA (Docosahexaenoic Acid) and application thereof - Google Patents
Aurantiochytrium sp. rich in glyceride type DHA (Docosahexaenoic Acid) and application thereof Download PDFInfo
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- CN103725617A CN103725617A CN201410000036.XA CN201410000036A CN103725617A CN 103725617 A CN103725617 A CN 103725617A CN 201410000036 A CN201410000036 A CN 201410000036A CN 103725617 A CN103725617 A CN 103725617A
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- 241001130339 Aurantiochytrium sp. Species 0.000 title claims abstract description 8
- 125000005456 glyceride group Chemical group 0.000 title claims abstract 7
- MBMBGCFOFBJSGT-KUBAVDMBSA-N all-cis-docosa-4,7,10,13,16,19-hexaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCC(O)=O MBMBGCFOFBJSGT-KUBAVDMBSA-N 0.000 title abstract 12
- 229940090949 docosahexaenoic acid Drugs 0.000 title abstract 6
- 235000020669 docosahexaenoic acid Nutrition 0.000 title abstract 6
- 150000002632 lipids Chemical class 0.000 claims abstract description 33
- 239000003653 coastal water Substances 0.000 claims abstract description 4
- 238000009629 microbiological culture Methods 0.000 claims abstract description 4
- 230000001580 bacterial effect Effects 0.000 claims description 8
- 244000005700 microbiome Species 0.000 claims description 8
- 229920001817 Agar Polymers 0.000 claims description 7
- 239000008272 agar Substances 0.000 claims description 7
- 239000002028 Biomass Substances 0.000 claims description 4
- 238000004108 freeze drying Methods 0.000 claims description 4
- 238000000926 separation method Methods 0.000 claims description 4
- 241000251468 Actinopterygii Species 0.000 claims description 3
- 238000004458 analytical method Methods 0.000 claims description 3
- 235000014113 dietary fatty acids Nutrition 0.000 claims description 3
- 239000000284 extract Substances 0.000 claims description 3
- 239000000194 fatty acid Substances 0.000 claims description 3
- 229930195729 fatty acid Natural products 0.000 claims description 3
- VOFUROIFQGPCGE-UHFFFAOYSA-N nile red Chemical compound C1=CC=C2C3=NC4=CC=C(N(CC)CC)C=C4OC3=CC(=O)C2=C1 VOFUROIFQGPCGE-UHFFFAOYSA-N 0.000 claims description 3
- 239000013535 sea water Substances 0.000 claims description 3
- 238000004440 column chromatography Methods 0.000 claims description 2
- 150000004665 fatty acids Chemical class 0.000 claims description 2
- 241000233675 Thraustochytrium Species 0.000 claims 4
- 235000008331 Pinus X rigitaeda Nutrition 0.000 claims 3
- 235000011613 Pinus brutia Nutrition 0.000 claims 3
- 241000018646 Pinus brutia Species 0.000 claims 3
- 241001298230 Thraustochytrium sp. Species 0.000 claims 1
- 239000003242 anti bacterial agent Substances 0.000 claims 1
- 229940088710 antibiotic agent Drugs 0.000 claims 1
- 150000002327 glycerophospholipids Chemical class 0.000 claims 1
- 239000001963 growth medium Substances 0.000 claims 1
- 238000010186 staining Methods 0.000 claims 1
- 150000003626 triacylglycerols Chemical class 0.000 claims 1
- 238000004321 preservation Methods 0.000 abstract description 4
- 241001306132 Aurantiochytrium Species 0.000 abstract 1
- 241001467333 Thraustochytriaceae Species 0.000 description 23
- OKKJLVBELUTLKV-UHFFFAOYSA-N Methanol Chemical compound OC OKKJLVBELUTLKV-UHFFFAOYSA-N 0.000 description 21
- HEDRZPFGACZZDS-UHFFFAOYSA-N Chloroform Chemical compound ClC(Cl)Cl HEDRZPFGACZZDS-UHFFFAOYSA-N 0.000 description 16
- 125000005908 glyceryl ester group Chemical group 0.000 description 10
- 235000021323 fish oil Nutrition 0.000 description 7
- IPCSVZSSVZVIGE-UHFFFAOYSA-N hexadecanoic acid Chemical compound CCCCCCCCCCCCCCCC(O)=O IPCSVZSSVZVIGE-UHFFFAOYSA-N 0.000 description 6
- VLKZOEOYAKHREP-UHFFFAOYSA-N n-Hexane Chemical compound CCCCCC VLKZOEOYAKHREP-UHFFFAOYSA-N 0.000 description 5
- 239000002904 solvent Substances 0.000 description 5
- RTZKZFJDLAIYFH-UHFFFAOYSA-N Diethyl ether Chemical compound CCOCC RTZKZFJDLAIYFH-UHFFFAOYSA-N 0.000 description 4
- 235000020673 eicosapentaenoic acid Nutrition 0.000 description 4
- 238000000605 extraction Methods 0.000 description 4
- 238000004519 manufacturing process Methods 0.000 description 4
- 235000013336 milk Nutrition 0.000 description 4
- 239000008267 milk Substances 0.000 description 4
- 210000004080 milk Anatomy 0.000 description 4
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 4
- 235000021314 Palmitic acid Nutrition 0.000 description 3
- 238000004587 chromatography analysis Methods 0.000 description 3
- 238000011081 inoculation Methods 0.000 description 3
- 239000007788 liquid Substances 0.000 description 3
- 239000000203 mixture Substances 0.000 description 3
- 239000000843 powder Substances 0.000 description 3
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 description 2
- 241000894006 Bacteria Species 0.000 description 2
- 239000002253 acid Substances 0.000 description 2
- 239000003795 chemical substances by application Substances 0.000 description 2
- 238000013375 chromatographic separation Methods 0.000 description 2
- 235000019688 fish Nutrition 0.000 description 2
- 238000000799 fluorescence microscopy Methods 0.000 description 2
- 239000007789 gas Substances 0.000 description 2
- 238000009776 industrial production Methods 0.000 description 2
- 230000003834 intracellular effect Effects 0.000 description 2
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- 230000000050 nutritive effect Effects 0.000 description 2
- 238000005809 transesterification reaction Methods 0.000 description 2
- 108020004463 18S ribosomal RNA Proteins 0.000 description 1
- FFRBMBIXVSCUFS-UHFFFAOYSA-N 2,4-dinitro-1-naphthol Chemical compound C1=CC=C2C(O)=C([N+]([O-])=O)C=C([N+]([O-])=O)C2=C1 FFRBMBIXVSCUFS-UHFFFAOYSA-N 0.000 description 1
- 241000555825 Clupeidae Species 0.000 description 1
- 108090000790 Enzymes Proteins 0.000 description 1
- 102000004190 Enzymes Human genes 0.000 description 1
- 241000233866 Fungi Species 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- 239000001888 Peptone Substances 0.000 description 1
- 108010080698 Peptones Proteins 0.000 description 1
- VYPSYNLAJGMNEJ-UHFFFAOYSA-N Silicium dioxide Chemical compound O=[Si]=O VYPSYNLAJGMNEJ-UHFFFAOYSA-N 0.000 description 1
- VMHLLURERBWHNL-UHFFFAOYSA-M Sodium acetate Chemical compound [Na+].CC([O-])=O VMHLLURERBWHNL-UHFFFAOYSA-M 0.000 description 1
- HXWJFEZDFPRLBG-UHFFFAOYSA-N Timnodonic acid Natural products CCCC=CC=CCC=CCC=CCC=CCCCC(O)=O HXWJFEZDFPRLBG-UHFFFAOYSA-N 0.000 description 1
- 210000000577 adipose tissue Anatomy 0.000 description 1
- 230000032683 aging Effects 0.000 description 1
- JAZBEHYOTPTENJ-JLNKQSITSA-N all-cis-5,8,11,14,17-icosapentaenoic acid Chemical compound CC\C=C/C\C=C/C\C=C/C\C=C/C\C=C/CCCC(O)=O JAZBEHYOTPTENJ-JLNKQSITSA-N 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 239000002551 biofuel Substances 0.000 description 1
- 229940088623 biologically active substance Drugs 0.000 description 1
- 229940041514 candida albicans extract Drugs 0.000 description 1
- 235000021466 carotenoid Nutrition 0.000 description 1
- 150000001747 carotenoids Chemical class 0.000 description 1
- 238000011109 contamination Methods 0.000 description 1
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- 239000000975 dye Substances 0.000 description 1
- 238000004043 dyeing Methods 0.000 description 1
- 229960005135 eicosapentaenoic acid Drugs 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 150000004676 glycans Chemical class 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 239000004615 ingredient Substances 0.000 description 1
- 238000002955 isolation Methods 0.000 description 1
- 238000011068 loading method Methods 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- WCYAALZQFZMMOM-UHFFFAOYSA-N methanol;sulfuric acid Chemical compound OC.OS(O)(=O)=O WCYAALZQFZMMOM-UHFFFAOYSA-N 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 229910052757 nitrogen Inorganic materials 0.000 description 1
- 238000002414 normal-phase solid-phase extraction Methods 0.000 description 1
- 239000006916 nutrient agar Substances 0.000 description 1
- 235000015097 nutrients Nutrition 0.000 description 1
- 235000020660 omega-3 fatty acid Nutrition 0.000 description 1
- 235000019319 peptone Nutrition 0.000 description 1
- 229920001282 polysaccharide Polymers 0.000 description 1
- 239000005017 polysaccharide Substances 0.000 description 1
- 238000004445 quantitative analysis Methods 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 239000001044 red dye Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 235000019512 sardine Nutrition 0.000 description 1
- 238000012163 sequencing technique Methods 0.000 description 1
- 239000000741 silica gel Substances 0.000 description 1
- 229910002027 silica gel Inorganic materials 0.000 description 1
- 235000017281 sodium acetate Nutrition 0.000 description 1
- 239000001632 sodium acetate Substances 0.000 description 1
- UFTFJSFQGQCHQW-UHFFFAOYSA-N triformin Chemical compound O=COCC(OC=O)COC=O UFTFJSFQGQCHQW-UHFFFAOYSA-N 0.000 description 1
- 239000012138 yeast extract Substances 0.000 description 1
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- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to an aurantiochytrium (i) sp. which is bred and is used for producing glyceride type DHA (Docosahexaenoic Acid), and an application thereof. The aurantiochytrium sp. is separated from coastal waters of Shenzhen of China and is preserved in China General Microbiological Culture Collection Center (CGMCC) on December 13, 2013; the preservation number is CGMCC No.8575. The content of non-polar lipids of an aurantiochytrium sp. strain in total lipids is high and the aurantiochytrium sp. is the strain rich in the glyceride type DHA and can be applied to industrially producing the glyceride type DHA.
Description
Technical field
The present invention relates to microorganism thraustochytriale
aurantiochytriumsp. CGMCC No. 8575, and the main component of its lipid is non-polar lipid, and wherein to account for total lipid content high for DHA, can obtain the glyceryl ester type DHA of high yield, are applicable to the industrial production of glyceryl ester type DHA.
Background technology
Polyunsaturated fatty acid (polyunsaturated fatty acids, PUFAs) be to keep one of important nutritive ingredient of HUMAN HEALTH, particularly DHA and timnodonic acid (eicosapentaenoic acids, EPA) have very important medical applications and nutritive value.
In food service industry, glyceryl ester type DHA can be added in milk or milk powder, as functional nutrient material.
At present, the commercial source of DHA is mainly deep sea fish oil, and sardines, fin fish etc. contains higher fatty tissue, in a lot of countries, is all used to produce fish oil, and in fish oil, the content of DHA can reach 20-30%.But the fish oil of take has a lot of shortcomings as raw material production DHA, as unstable in fish oil output, containing having a fish like smell, may there is heavy metal contamination in complicated component.
In ocean environment, microorganism is only the original producer of DHA as marine microalgae, fungi etc.In these microorganisms, the relative content of DHA will be far above the content in fish oil.
Thraustochytriale is the Mycophyta protobiont of a class heterotrophism, in recent years because its wide biotechnology applications prospect receives much concern.Thraustochytriale can be used for producing extracellular enzyme, exocellular polysaccharide, carotenoid, omega-3 polyunsaturated fatty acids as DHA and EPA, and the biologically active substance of some other industrial application.
The lipid acid of thraustochytriale forms simply, culture condition cost is low, utilizes thraustochytriale to produce DHA, can effectively solve the problem that fish oil DHA exists.Therefore,, as the new production source of polyunsaturated fatty acid, thraustochytriale is to have the microorganism that potentiality are carried out suitability for industrialized production DHA.
Summary of the invention
The object of the present invention is to provide the high thraustochytriale of glyceryl ester type DHA content
aurantiochytriumsp. CGMCC No. 8575, can be used for suitability for industrialized production DHA, make an addition in milk, milk powder or healthcare products.
According to the present invention, provide a kind of glyceryl ester type DHA output high, be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (China General Microbiological Culture Collection Center, CGMCC), deposit number is the thraustochytriale of CGMCC No. 8575.The Classification And Nomenclature of this bacterial strain is
aurantiochytriumsp.; Depositary institution address is No. 3, Yard 1, BeiChen xi Road, Chaoyang District, Beijing City, Institute of Microorganism, Academia Sinica; Preservation date is on December 13rd, 2013.
This bacterial strain is in July, 2012, separated from Tai Pang Wan coastal waters, Shenzhen.The feature of separation method is: adopt different antibiotic combinations, utilize the Pollen Pini seed selection of fishing.When thraustochytriale is enriched in Pollen Pini edge, picking preserved egg powder, streak culture on agar plate, after 3-5 days, picking list bacterium colony is streak culture on new agar plate, to obtain purebred thraustochytriale.According to this separation method, from a sampling point, obtain altogether 8 strain thraustochytriales.
In the present invention, relate to extraction and the analysis of thraustochytriale lipid.Its feature is: utilize chloroform: the mixture of methyl alcohol=1:2 system, as extraction solvent, extracts intracellular TL, by chromatography of gases, result is analyzed, thereby is selected the bacterial strain that DHA content is high,
aurantiochytriumsp. CGMCC No. 8575.
In the present invention, the isolation identification that also comprises thraustochytriale non-polar lipid.Utilize Nile red dyeing, at fluorescence microscopy Microscopic observation, show thraustochytriale
aurantiochytriumsp. in CGMCC No. 8575 born of the same parents, non-polar lipid content is high, and quantitative analysis shows, the thraustochytriale of freeze-drying
aurantiochytriumsp. CGMCC No. 8575 every gram comprise 0.65-0.85g lipid, wherein approximately have 75% for non-polar lipid, wherein glyceryl ester type DHA accounts for 73% of total DHA.
According to the present invention, thraustochytriale
aurantiochytriumsp. in CGMCC No. 8575 cells, lipid acid is mainly DHA and hexadecanoic acid, the 80-90% that both sums are fatty acid content, and hexadecanoic acid content can reach 35-65%.The hexadecanoic acid of high-content illustrates that this bacterial strain is also applicable to the industrial production of biofuel.
Embodiment
The present invention found to be present in the thraustochytriale in China Shenzhen coastal waters, and selected the bacterial strain that glyceryl ester type DHA is rich in a strain.18S rRNA sequencing result shows that this thraustochytriale is
aurantiochytriumsp., this bacterial strain is named as
aurantiochytriumsp. PKU#SW7, is preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center (China General Microbiological Culture Collection Center, CGMCC), and deposit number is CGMCC No. 8575.
Thraustochytriale
aurantiochytriumsp. CGMCC No. 8575 is preserved on agar plate.Nutrient agar is composed as follows: in every 100ml filtering sea, contain glucose 1g, peptone 0.15g, yeast extract 0.01g, agar 1g.By streak culture thraustochytriale
aurantiochytriumsp. CGMCC No. 8575 is placed in room temperature preservation.
Can prepare inoculation culture liquid according to following scheme.
The well-grown bacterium colony of picking from the agar plate of preservation, cultivates in access 10-20ml sea water medium, as inoculation liquid.Inoculation liquid, after cultivating 2 days, is inoculated in fresh sea water medium to be greater than 5% ratio.Culture condition temperature is 25-30 ℃, and rotating speed is 160rpm, after cultivating 4 days, and biomass collection.
In culturing process, utilize Nile red dye to thraustochytriale
aurantiochytriumsp. CGMCC No. 8575 cells dye, and in fluorescence microscopy Microscopic observation, its non-polar lipid content of the golden yellow fluorescence display in cell paste is high.
Take a certain amount of freeze-drying biomass samples, can utilize chloroform: the mixture of methyl alcohol=1:2 system, as extraction solvent, extracts intracellular TL.First, the dried biomass taking is placed in tool plug triangular flask, add after extraction solvent, chloroform is added in 160rpm vibration after 1 hour: methyl alcohol=1:1 system solvent, mix rear standing 1h, and migrate out chloroform layer, nitrogen blows the concentrated TL that obtains.The 65-85% that acquisition total lipid content is dry cell weight, i.e. every gram of thraustochytriale
aurantiochytriumsp. the amount that CGMCC No. 8575 freeze drying cell contain lipid is 0.65-0.85g.
The TL extracting is carried out to column chromatography separation.Adopt sigma Discovery DSC-NH
2solid-phase extraction column, utilizes the aging pillar of normal hexane, with normal hexane: the system loading of chloroform: methyl alcohol=95:3:2.First non-polar lipid part obtains by chloroform wash-out, and polar lipid part obtains by two step wash-outs, and the first step adopts methyl alcohol: chloroform=6:1 wash-out, second step adopts 0.05M sodium acetate soln, and (solvent is methyl alcohol: chloroform=6:1) wash-out.Result shows that non-polar lipid accounts for 75% left and right of TL.
The non-polar lipid that coupled columns chromatographic separation obtains is carried out thin-layer chromatographic analysis.Adopt G type tlc silica gel plate, carry out two-dimentional chromatographic separation.In first direction, adopting normal hexane: ether=80:20 is developping agent, and second direction adopts normal hexane: ether: methyl alcohol=70:20:10 is developping agent.Result shows that the main component in non-polar lipid is triglyceride level.
TL, non-polar lipid and polar lipid are carried out respectively to transesterification.Adopt 4% sulfuric acid methanol solution, in 80 ℃ of transesterification reactions 1 hour, be cooled to after room temperature, use normal hexane extracting, for chromatography of gases analysis.Find that DHA accounts for the 28-35% of total lipid content, in non-polar lipid, the content of DHA is 20-25%, and glyceryl ester type DHA reaches 73% left and right of total DHA content, shows thraustochytriale
aurantiochytriumsp. CGMCC No. 8575 is bacterial strains that a strain is highly suitable for producing glyceryl ester type DHA.
Claims (7)
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111718858A (en) * | 2020-07-12 | 2020-09-29 | 天津大学 | Thraustochytrium fatty acid production method based on nitrogen-limited culture and phytohormone regulation |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101370929A (en) * | 2005-12-16 | 2009-02-18 | 阿维沙基因有限公司 | Thraustochytrium strain-SC1 for producing Docosahexaenic Acid |
| CN102428185A (en) * | 2009-03-19 | 2012-04-25 | 马太克生物科学公司 | Thraustochytrid, fatty acid composition and preparation method and application thereof |
| CN103025862A (en) * | 2010-05-04 | 2013-04-03 | 韩国生命工学研究院 | Novel thraustochytrid-based microalgae, and method for preparing bio-oil by using same |
| CN103789213A (en) * | 2013-11-07 | 2014-05-14 | 北京大学深圳研究生院 | A thraustochytrium Aurantiochytrium PKU#SW7 rich in glyceride-type DHA and its application |
-
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- 2014-01-01 CN CN201410000036.XA patent/CN103725617A/en active Pending
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN101370929A (en) * | 2005-12-16 | 2009-02-18 | 阿维沙基因有限公司 | Thraustochytrium strain-SC1 for producing Docosahexaenic Acid |
| CN102428185A (en) * | 2009-03-19 | 2012-04-25 | 马太克生物科学公司 | Thraustochytrid, fatty acid composition and preparation method and application thereof |
| CN103025862A (en) * | 2010-05-04 | 2013-04-03 | 韩国生命工学研究院 | Novel thraustochytrid-based microalgae, and method for preparing bio-oil by using same |
| CN103789213A (en) * | 2013-11-07 | 2014-05-14 | 北京大学深圳研究生院 | A thraustochytrium Aurantiochytrium PKU#SW7 rich in glyceride-type DHA and its application |
Non-Patent Citations (1)
| Title |
|---|
| 李晶晶等: "破囊壶菌生产DHA的应用前景", 《食品工业科技》 * |
Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111718858A (en) * | 2020-07-12 | 2020-09-29 | 天津大学 | Thraustochytrium fatty acid production method based on nitrogen-limited culture and phytohormone regulation |
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