CN103690582B - A kind of compositions and application thereof containing dendrobium polysaccharide and atractylis concrete - Google Patents

Abstract

A kind of compositions containing dendrobium polysaccharide and atractylis concrete.Described compositions contains treatment or prevents the dendrobium polysaccharide of effective dose, atractylis concrete and pharmaceutically acceptable pharmaceutic adjuvant, and said composition can be applicable to prevention or alleviates the medicine of gastric ulcer or the preparation of health food.

Description

A composition containing dendrobium polysaccharide and atractylodes stearin and its application

technical field

The invention belongs to the field of medicine and food composition, in particular, relates to a medicine or food composition containing dendrobium polysaccharide and atractylodes stearin, a preparation method of the composition and its application in the preparation of medicine for treating and/or preventing gastric ulcer or food applications.

technical background

The traditional Chinese medicine Dendrobium is a general term for various medicinal plants of the Orchidaceae plant Dendrobium (Dendrobium). Dendrobium was first recorded in Shennong’s Herbal Classic. It has the functions of nourishing the stomach and promoting body fluid, nourishing yin and clearing heat, and improving eyesight. For decades, Chinese and foreign scholars have conducted a lot of research on the chemical components and pharmacological effects of Dendrobium plants, and found that there are various types of chemical components in Dendrobium plants; pharmacological studies have proved that Dendrobium has anti-aging, anti-tumor, and blood sugar-lowering effects. It is also very effective in treating gastrointestinal diseases and treating cataracts.

According to reports, there are about 63 species of Dendrobium plants in China, mainly distributed in South China and Southwest China, and there are more than 30 species of Dendrobium plants for medicinal purposes (Zhang Jili, He Jinli. Advances in Pharmacological Research of Dendrobium. Shizhen Guoyi Guoyao. 2000 ; 11(5):469-470). The 2000 edition of "Pharmacopia of the People's Republic of China" recorded Dendrobium as Dendrobium glabra (Dendrobium ring grass, Pharmacopoeia record name, the same below) D. Fresh or dried stems of D. chrysanthum (Dendrobium officinale), D. officinale (D. candidum) and D. nobile (Dendrobium nobile) (Zhang Guangnong, et al. Research progress on chemical constituents of Dendrobium genus. Chinese herbal medicine .2003;34(6):Appendix 5-8).

According to reports, research on the chemical components of a variety of Dendrobium plants has found that the chemical components contained in this genus are diverse. In addition to alkaloids, there are phenanthrenes, bibenzyls, fluorenones, sesquiterpenes, aromatic Soybein, polysaccharides, steroids and volatile oils, etc. (Zhang Guangnong, et al. Research progress on chemical constituents of Dendrobium genus. Chinese herbal medicine. 2003;34(6):attachment 5-8).

Dendrobium polysaccharides refer to polysaccharides extracted from plants of the genus Dendrobium. Studies have reported that polysaccharides from Dendrobium candidum have immunomodulatory and blood sugar-regulating activities (Liu Li, et al. Research progress on polysaccharides from medicinal plants of the genus Dendrobium. Modern Chinese Medicine Research and Practice. 2009;23(1):77-80).

According to the "Chinese Pharmacopoeia" (2010 edition), Atractylodes refers to the dry rhizomes of Atractylodes lancea and Atractylodeschinensis of the genus Atractylodes atractylodes in the family Asteraceae. In addition, plants of the same genus used as medicinal plants also include Atractylodes japonica, A. koreana, A. chinensis, and A. chinensis.

Cangzhu has been used in traditional Chinese medicine for thousands of years. According to traditional Chinese medicine literature, Atractylodes atractylodes is pungent, bitter, and warm in nature, and belongs to the spleen, stomach, and liver meridian. Mainly used for abdominal fullness, diarrhea, edema, beriberi flaccidity, rheumatic arthralgia, anemofrigid cold, night blindness of birds.

Atractylodes stearin, also known as Atractyllisoil, is a volatile oil extracted from the dried rhizome of Atractylodes atractylodis. The commonly used extraction method of Atractylodes atractylodes is steam distillation, and its yield is about 3% to 8%. In food and daily chemicals, atractylodes stearin is mainly used as a fixative.

Traditional Chinese medicine believes that Atractylodes atractylodis is dry in nature, and bran-stir-frying is one of the main medicinal materials processing methods of Atractylodes atractylodes. Preliminary Exploration. Shi Zhen Guo Yi Guo Yao. 2001; l2(3):257-258).

It has been reported in the literature that Atractylodes volatile oil has antibacterial and sterilizing effects, and can regulate the gastrointestinal motility function of experimental animals.

Gastric ulcer is the most common type of peptic ulcer, which mainly refers to tissue damage beyond the muscularis mucosa caused by the gastric mucosa being digested by the gastric digestive juice itself. With the increase of people's social pressure and irregular diet, more and more people suffer from chronic gastric ulcer. Except for a few patients who went to the hospital earlier, most of the patients' course of disease can last for several years, ten years or longer. And the recurrence rate is extremely high.

Modern medical research believes that the most important pathological factors of gastritis and gastric ulcer include hypersecretion of gastric acid, Helicobacter pylori infection and so on. Among them, Helicobacter pylori (HP) infection is the main cause of chronic gastritis and an important cause of peptic ulcer. In HP-adhered epithelial cells, microvilli decreased, intercellular junctions lost, cells swelled, surface irregularities, intracellular mucus granules depleted, vacuolar changes, adhesion pedicles and shallow cup-like structures formed between bacteria and cells.

The etiology and pathology of gastritis and gastric ulcer have been studied more and more in modern medicine, and Western medicine has significantly improved the diagnosis and treatment of gastritis and peptic ulcer. Treatment measures for peptic ulcer include emotional, dietary adjustment, and drug therapy. Antacids such as sodium bicarbonate, aluminum hydroxide gel, bismuth subcarbonate, etc. can reduce the acidity of the stomach and duodenum, relieve pain, and promote ulcer healing. Anticholinergics such as atropine, probenzine, and metoclopramide can inhibit the vagus nerve and reduce gastric acid secretion, but delaying gastric emptying can cause increased secretion of gastrin. Histamine H2 receptor antagonists such as cimetidine and famotidine compete with histamine for _H2 receptors and eliminate the effect of histamine on gastric acid secretion. Acid pump inhibitors such as omeprazole and lansoprazole and H. pylori-inhibiting drugs such as metronidazole and rifaxigen constitute triple therapy for the treatment of H. pylori-positive peptic ulcer disease.

The clinical manifestations of gastritis and gastric ulcer belong to the categories of "noisy", "stomach fullness", "acid regurgitation", "epigastric pain" and "heart pain" in traditional Chinese medicine. Western medicine pays attention to symptomatic treatment, mainly to repair the ulcer surface, eliminate inflammation, kill Helicobacter pylori and other measures, the course of treatment is long, and the side effects are large. Traditional Chinese medicine believes that the spleen and the stomach are external and internal, the spleen governs the promotion of clearness, and the stomach governs the suppression of descending. Disharmony between the spleen and the stomach or the pathogenesis of liver qi attacking the stomach can cause stomach problems. Methods such as harmonizing the spleen and stomach and dredging liver qi can treat stomach diseases as a whole.

So far, in the prior art, there is no report on the composition prepared by mixing Atractylodes stearin and Dendrobium polysaccharide, and there is no report that the composition can produce synergistic anti-ulcer effect.

Contents of the invention

The purpose of the present invention is to provide a composition prepared by mixing Atractylodes stearin and Dendrobium polysaccharides. The present invention mixes Atractylodes stearin and Dendrobium polysaccharides in a molten state to prepare a solid mixture at room temperature, and the resulting compound can produce synergistic antibacterial Ulcer effects, especially for the prevention and treatment of experimental gastric ulcer models caused by Helicobacter pylori. In addition, the present invention also provides a preparation method of the composition and its application in the preparation of medicine or food for treating and/or preventing gastric ulcer.

Following technical scheme of the present invention is for realizing above-mentioned purpose of the invention:

The present invention firstly provides an edible and/or pharmaceutical composition, which contains an effective dose of Dendrobium polysaccharide extract, Atractylodes stearin and physiologically acceptable excipients for preventing and/or treating gastric ulcer, wherein:

The Dendrobium polysaccharide is the total polysaccharide extracted from Dendrobium candidum and/or Dendrobium nobile, and the content of sugar components in the Dendrobium polysaccharide extract is not less than 80% of the total amount of the extract, The polysaccharide weight average molecular weight is not less than 20kD;

The atractylodes stearin is the total volatile oil extracted from the dry rhizome of Atractylodeslancea, wherein the sum of the contents of atractylodes alcohol and eucalyptol is not less than 50% of the total amount of volatile oil;

The composition ratio of the Dendrobium polysaccharide to Atractylodes stearin is in the range of 1:0.25 to 1:2.

The edible and/or pharmaceutical composition of the present invention means that the composition of the present invention can be used as a functional food, and can also be used as a pharmaceutical composition for preventing and treating peptic ulcer.

The dendrobium polysaccharide of the present invention can optionally be extracted from Dendrobium candidum and/or Dendrobium nobile. The research of the present invention shows that the polysaccharides of Dendrobium officinale and Dendrobium nobile can produce similar anti-gastric ulcer activity when used in the pharmaceutical composition of the present invention.

The chemical components of the volatile oils contained in the dried rhizomes of different species of Atractylodes genus are quite different (Wang Liuying, et al. Analysis and comparison of volatile components of Atractylodes herb. Shizhen Guoyi Guoyao 2009;20(4):924-925) . Atractylodes stearin in the present invention refers to the volatile oil extracted from the dry rhizome of Atractylodes atractylodes, also known as Atractylodeslances. In the atractylodes stearin, the sum of the contents of hinesol and β-eudesmol by weight ratio is not less than 50% of the total amount of volatile oil. Atractylodes alcohol (hinesol) and eucalyptol (β-eudesmol) are shown in the figure below.

The atractylodes alcohol and β-cineoleol contained in Atractylodes stearin can be confirmed by gas chromatography-mass spectrometry, and their content can be detected by gas chromatography.

According to the research of the present invention, when the composition ratio of Dendrobium polysaccharides and Atractylodes stearin is in the range of 1:0.25-1:2, the compositions all have good synergistic anti-experimental gastric ulcer activity. In a preferred embodiment of the present invention, the composition ratio of Dendrobium polysaccharide and Atractylodes stearin in the pharmaceutical composition is in the range of 3:0.5-1:1.

The present invention also provides a preparation method of the dendrobium polysaccharide, the method comprising the steps of:

(1) water extraction method obtains the dendrobium water extract;

(2) obtaining the total polysaccharides of Dendrobium dendrobii through C1-C4 lower alcohol or ketone fractional precipitation with the aqueous solution of the dendrobium water extract obtained in step (1);

(3) The dendrobium polysaccharide extract obtained in step (2) can be further optionally purified by decolorization, ultrafiltration or chromatographic chromatography.

In the step (1), the water extraction method is generally about 8 to 15 times the weight of water, and the extraction is performed 2 to 3 times at room temperature to 100 ° C; the resulting aqueous solution can be optionally concentrated properly and then proceed to the next step deal with;

In step (2), preferred lower alcohols and ketones include ethanol and acetone, and the obtained precipitate can optionally be washed 1 to 3 times with lower alcohols and ketones, and then dried by vacuum drying or freeze drying.

The total polysaccharide obtained in step (2) can be directly used in the preparation of the composition of the present invention. Those skilled in the art can understand that the total polysaccharides can also be further purified by decolorization, ultrafiltration, gel chromatography, and ion exchange chromatography.

According to the present invention's repeated test research results, the neutral sugar content in the polysaccharide extract of Dendrobium dendrobii is not less than 80% of the total extract; Due to different production batches) and the degree of purification, the components of the obtained Dendrobium polysaccharides are not the same, but generally, the average molecular weight is not less than about 20kD.

The present invention further provides a preparation method of Atractylodes stearin, which can optionally adopt (1) steam distillation method, (2) supercritical extraction method, (3) organic solvent extraction method to extract and obtain the total volatile oil, that is, the present invention The described Atractylodes stearin.

The volatile oil extraction method is well known to those skilled in the art, wherein steam distillation is a commonly used volatile oil extraction method; and _CO2 supercritical extraction can improve the extraction yield of volatile oil, and may contain higher content of fat in its product esters, those skilled in the art can understand that further methods such as crystallization and solvent extraction can reduce or partially remove the content of fatty acids and esters in supercritical extraction products; low-level organic solvents such as cyclohexane extraction can also obtain Atractylodes stearin required by the present invention.

The present invention also provides a method for preparing a solid preparation of the pharmaceutical composition, the steps comprising:

(1) At 60°C to 80°C, make the prepared amount of Atractylodes stearin in a molten state, stir and add the prepared amount of Dendrobium polysaccharide, refrigerate at -18°C to 4°C to solidify, and crush at low temperature;

(2) adding a physiologically acceptable excipient to the product obtained in step (1) and granulating;

(3) Compressing the granules obtained in step (2) into tablets or filling capsules.

Generally speaking, due to its oily nature, plant volatile oil components are mostly prepared into soft capsule preparations, and it is difficult to prepare solid preparations. The present invention finds that the polysaccharide of Dendrobium candidum is stirred and mixed in the stearin of Atractylodes atractylodes in molten state, under the condition of lowering the temperature, an easily pulverized solid can be obtained, and after the solid is pulverized, it can be easily mixed with pharmaceutically acceptable excipients. The powder or granule of the obtained mixture can have good fluidity, and can be easily granulated with preparation auxiliary materials, so it can be easily filled into capsules or compressed into tablets.

The pharmaceutically acceptable excipients may include but not limited to fillers, slip agents, binders, colorants and the like. For solid tablets, coatings such as coating agents and coloring agents can also be optionally used.

The study of the present invention proves that the Dendrobium polysaccharide-containing Atractylodes stearin composition of the present invention has the effect of preventing and/or treating ulcer in the gastric ulcer model caused by pyloric ligation and Helicobacter pylori, and its effect presents obvious synergistic effect effect, so the present invention further provides the use of the pharmaceutical composition of the present invention in the preparation of drugs for the prevention and/or treatment of gastric ulcer.

Description of drawings

Fig. 1 is the High Performance Gel Chromatography (HPGPC) collection of collections of Dendrobium officinale polysaccharide and Dendrobium nobile polysaccharide, (A: Dendrobium officinale polysaccharide; B: Dendrobium nobile polysaccharide);

Figure 2 is the GC-MS spectrum of Atractylodes stearin, (A: GC spectrum; B: MS spectrum of atractylodes alcohol; C: MS spectrum of β-cineoleol).

detailed description

The present invention will further illustrate the substantive content of the present invention with specific embodiments below in conjunction with the accompanying drawings. However, these specific examples are only for illustrating the present invention, and do not constitute any limitation to the scope of the present invention.

Example 1:

Dendrobium polysaccharide preparation

Take 1000g of the dry aerial parts of Dendrobium candidum and Dendrobium nobile, soak in 95% (V/V) ethanol to degrease, centrifuge to remove ethanol, extract twice with 12L water at 90°C, combine the water extracts, and concentrate under reduced pressure to the extract The total volume is about 8L, add 24L of 95% (V/V) ethanol, let it stand overnight at 4°C, and centrifuge to obtain a precipitate. Dissolve the obtained precipitate with 3L of pure water, centrifuge to remove the insoluble matter, adjust the supernatant to pH 2.8 with 2N HCl, let it stand at 4°C for about 4 hours, centrifuge to remove the precipitate, adjust the pH to 9 with 2N NaOH, add 90ml of 30% H ₂ O ₂ , 60°C After stirring and decolorizing for 1 hr, add 9L of 95% (V/V) ethanol, let it stand overnight at 4°C, centrifuge to obtain a precipitate, wash the resulting precipitate twice with 2L of 90% ethanol, and dry under reduced pressure at room temperature to obtain about 132.7g and 38.5g.

Get Dendrobium officinale total polysaccharide 80g and dissolve in 2000ml pure water, 3kD ultrafiltration membrane (Millipore ultrafiltration device) ultrafiltration, intercept solution and freeze-dry to obtain Dendrobium officinale polysaccharide 52.6g;

Another 25 g of total polysaccharides of Dendrobium nobile was taken, dissolved in 800 ml of pure water, purified by Sephadex-G25 gel chromatography column (Ф8 cm x 150 cm), and the sugar-containing components were collected and freeze-dried to obtain 15.3 g of total polysaccharides of Dendrobium nobile.

The total polysaccharides of Dendrobium officinale, Dendrobium officinale polysaccharides, Dendrobium nobile total polysaccharides and Dendrobium nobile polysaccharides were detected by the sulfuric acid phenol method to be 81.3%, 88.7%, 82.6% and 92.8%, respectively.

Taking standard dextran as standard, high performance gel chromatography (HPGPC) method detects described Dendrobium officinale polysaccharide and Dendrobium nobile polysaccharide, as seen it is the mixed polysaccharide of multicomponent, and the weight-average molecular weight of polysaccharide component is all greater than 20kD ( See Fig. 1A,B).

Example 2:

Atractylodes stearin preparation

Method: Take three 1kg of crushed dry rhizome granules of Atractylodes Lances and use them for steam distillation extraction, organic solvent extraction and CO ₂ supercritical extraction respectively. in:

(1) Steam distillation extraction is to put 1 kg of dried rhizome granules of Atractylodes atractylodes into the grinding bottle of the volatile oil extractor, and continue to distill for 5 hours according to the method in the appendix of the Chinese Pharmacopoeia, then stop heating, drain water after cooling, and collect the oil on the surface of the distillate things.

(2) The organic solvent extraction method is to put 1 kg of dried rhizome particles of Atractylodes atractylodes into a flask, use n-hexane as a solvent, extract under reflux for 8 hours, and distill off the solvent under reduced pressure to obtain an extraction product.

(3) The CO ₂ supercritical extraction method is to put 1 kg of dried rhizome particles of Atractylodes atractylodes into a supercritical fluid extractor with a flow rate of 35 kg/h, a temperature of 50 °C, and a pressure of 20 MPa for static extraction for 1 hr and dynamic extraction for 3 hr. At separation pressure ~ 10MPa and temperature 40°C, volatile oil is precipitated.

Gas chromatography analysis method (GC) detects the content of atractyl alcohol and β-cineole alcohol in three kinds of extraction method gained Atractylodes stearin, and the chromatographic condition of described detection is, HP-5MS chromatographic column, quartz capillary column (30m * 250 μ m ×0.25μm); gasification chamber temperature 280°C; mass spectrometer (MS) detector, ion source temperature 230°C; quadrupole temperature 200°C; column temperature (programmed temperature rise): 60°C (3min), 10°C/min temperature rise To 150°C, 3°C/min to 220°C, 25°C/min to 280°C (10min); split ratio 10:1; carrier gas He, flow rate 1.0ml/min.

Results: About 32.5 g of Atractylodes stearin was obtained by steam distillation, and the obtained product was light yellow solid at low temperature (20°C or below). Detected by gas chromatography, the sum of the contents of atractyl alcohol and β-cineoleol is about 83.6% (wherein it contains about 48.8% atractyl alcohol and 34.8% β-cineoleol). The GC-MS collection of spectra of Atractylodis stearin obtained by steam distillation is shown in Figure 2. Figure 2A is the total ion chromatogram obtained by GC detection; 2B is the MS spectrum of atractylitol; 2C is the MS spectrum of β-eucalyptol.

About 25.1 g of Atractylodes stearin was obtained by n-hexane extraction, and the obtained product was viscous yellow oil. Detected by gas chromatography-mass spectrometry (GC-MS), the sum of the contents of atractyl alcohol and β-cineoleol is about 54.2%;

About 41.6g of Atractylodes stearin was obtained by _CO2 supercritical extraction, and the resulting product was light yellow solid at low temperature (20°C or below). Detected by gas chromatography-mass spectrometry (GC-MS), the sum of the contents of atractyl alcohol and β-cineole alcohol is about 69.2%.

Example 3:

Therapeutic effect of Dendrobium polysaccharide Atractylodes stearin composition on gastric ulcer model of pyloric ligation experimental animals:

Test sample: Dendrobium polysaccharide; Atractylodes stearin (extracted by steam distillation); Composition A, which is a mixture of Dendrobium officinale polysaccharide and steam distillation obtained Atractylodes stearin in a weight ratio of 1:0.5; Composition B, which is A mixture of Dendrobium nobile polysaccharide and Atractylodes stearin extracted with n-hexane at a weight ratio of 1:2; composition C, which is a mixture of Dendrobium nobile polysaccharide and supercritically extracted Atractylodes stearin at a weight ratio of 1:0.25.

Test method: 64 SD rats with a body weight of 180-220g were randomly divided into 8 groups (8 rats in each group), namely the model control group, the Dendrobium polysaccharide administration group, the Atractylodes stearin administration group, and the Dendrobium polysaccharide-Atractylodes water volatile oil composition Administration groups (A-200mg/kg, A-100mg/kg, B-200mg/kg, C-100mg/kg administration groups respectively), positive control group (ranitidine-50mg/kg).

Rats were fasted for 48 hours before the test without water. During the test, after the rats were anesthetized and fixed, the pylorus was ligated aseptically. After the operation, the animals in each test group were given the corresponding test drugs, the positive control group was given ranitidine, and the model group was given the same amount of solvent in the same way, and water and food were stopped.

Eighteen hours later, the rats were sacrificed, the abdominal cavity was opened, the esophagus near the diaphragm was ligated, and the stomach was fixed in 1% formaldehyde solution for 10 minutes. The stomach wall was cut open along the greater curvature of the stomach, the contents were cleaned, the stomach wall was unfolded and fixed on a wax board with a pin, the gastric mucosal surface was observed, and the number of ulcerated animals, ulcer area and ulcer index in each group were recorded according to conventional methods.

Test results: The test results are shown in Table 1.

The results showed that compared with the model group, the number of rats with ulcers in each administration group was reduced, the degree of ulcers was significantly reduced, the area of ulcers was reduced, and the index of ulcers was reduced.

The anti-ulcer results of composition A show that the pharmacodynamic effect of the Dendrobium polysaccharide-Atractylodes stearin composition is obviously better than that of the corresponding Dendrobium polysaccharide and Atractylodes volatile oil administered alone, and its drug effects have synergistic effects. Dynamics effect. The results of pharmacodynamic experiments of composition B and composition C showed that polysaccharides of Dendrobium candidum and Dendrobium nobile polysaccharide combined with Atractylodes stearin can produce synergistic effect; Fat has anti-ulcer effect.

Table 1. Animal model test results of rat pylorus-ligated gastric ulcer

Example 4:

Therapeutic effect of Dendrobium polysaccharide Atractylodes stearin composition on rat gastric ulcer model induced by Helicobacter pylori:

Test sample: Dendrobium polysaccharide Atractylodes stearin composition D, which is a mixture of Dendrobium officinale polysaccharide and Atractylodes stearin obtained by steam distillation at a weight ratio of 1:1.

Test method: 40 SD rats weighing 180-220 g. Rats were fasted for 24 hours, anesthetized with pentobarbital, laparotomed, dipped in glacial acetic acid with a diameter of 5.5 mm, soaked the serosal surface of the gastric antrum twice, each time for 30 s, washed the burnt part with physiological saline cotton ball 3 times, The abdominal cavity was sutured, and the patients were generally fed after operation. Three days after the operation, the rats were fasted for 24 hours and fed with Helicobacter pylori HP10 ⁹ CFUP/rat. After that, they were fed with HP every day for four consecutive days, a total of 5 times. After modeling, the rats were randomly divided into 4 groups (10 in every group), i.e. model control group, composition D administration group of 250mg/kg and 125mg/kg dosage, positive control group (bismuth potassium citrate tablet / tinidazole tablets/ clarithromycin tablets, 75mg/kg). Each administration group was given intragastric administration for 7 consecutive days, and the model group was given an equal volume of normal saline. The animals were sacrificed 2 weeks after the last drug administration, and the stomach was taken for observation and the ulcer index was calculated; at the same time, gastric antrum tissue was taken for urease-dependent test (RUT) to check the existence of Hp in experimental animals.

Experimental results: Dendrobium polysaccharide Atractylodes stearin composition D has a significant inhibitory effect on HP infection, and has a good therapeutic effect on the glacial acetic acid burn-HP infected ulcer model.

Table 2. Animal model test results of rat pylorus-ligated gastric ulcer

Example 5:

Preparation of solid tablet of Dendrobium polysaccharide Atractylodes stearin composition:

prescription

Preparation method: melting Atractylodes atractylodis oil at 60°C, adding Dendrobium polysaccharide during stirring, standing overnight at 0°C, pulverizing the obtained solids (80 mesh), adding the prescribed amount of microcrystalline cellulose, mixing and granulating, and adding the prescribed amount of the obtained granules to harden Magnesium fatty acid is mixed, and compressed into tablets by conventional methods.

Example 5:

Hard capsule preparation of Dendrobium polysaccharide Atractylodes atractylodes stearin composition:

prescription

Preparation method: Melt the prescribed amount of Atractylodes stearin at 60°C, add the prescribed amount of polyvinylpyrrolidone (PVPK90) and Dendrobium polysaccharide while stirring at 60°C, continue to stir for 30 minutes, place it at 0°C overnight, and crush the obtained solids (80 mesh), add prescription amount of lactose and microcrystalline cellulose, mix evenly, add pre-prepared HPMC solution to make soft material, granulate with 20 mesh sieve, dry at 60°C for about 30 minutes, granulate with 18 mesh sieve, pack 2 ^#Capsules are enough.

The tablets and capsules of the above-mentioned Examples 5 and 6 can also be used in food (health food).

Claims (7)

1. a compositions, containing treatment or the dendrobium polysaccharide of preventing gastric ulcer effective dose, atractylis concrete and pharmacologically acceptable excipient, wherein:

Described dendrobium polysaccharide is the total polysaccharides that the content extracted from Herba Dendrobii Dendrobiumcandidum and/or Dendrobium nobile Dendrobiumnobile is not less than extract total amount 80%, polysaccharide weight average molecular weight is not less than 20kD;

Described atractylis concrete is the total volatile oil extracted from Rhizoma Atractylodis Atractylodeslancea, and wherein the content sum of atractylol and eudesmol is not less than 50% of volatile oil total amount;

The composition weight percent of described dendrobium polysaccharide and atractylis concrete is in the scope of 1:0.25 to 1:2.

2. compositions as claimed in claim 1, the composition weight percent of wherein said dendrobium polysaccharide and atractylis concrete is in the scope of 1:0.5 to 1:1.

3. compositions as claimed in claim 1 or 2, wherein the preparation method of dendrobium polysaccharide comprises:

(1) water extraction method obtains Herba Dendrobii water extract;

(2) aqueous solution of step (1) gained Herba Dendrobii water extract is obtained Herba Dendrobii crude polysaccharides through C2-C4 lower alcohol or ketone fractional precipitation;

(3) step (2) gained Herba Dendrobii crude polysaccharides is carried out decolour, ultrafiltration or chromatographic purification obtain dendrobium polysaccharide.

4. compositions as claimed in claim 1 or 2, wherein the preparation method of atractylis concrete is steam distillation or supercritical extraction or organic solvent extraction.

5. the solid preparation preparation method of compositions described in claim 1 or 2, comprising:

Make the atractylis concrete of preparation effective dose be molten condition at (1) 60 DEG C ~ 80 DEG C, stir the dendrobium polysaccharide adding amount of formulation ,-18 DEG C ~ 4 DEG C cold preservations make it to solidify, pulverize at low temperature;

(2) pharmacologically acceptable pharmaceutical excipient granulating is added in step (1) products therefrom;

(3) by step (2) gained granulation or filled capsules.

6. compositions described in claim 1 or 2 is prevented in preparation or is treated the application in the medicine of gastric ulcer.

7. the application of compositions described in claim 1 or 2 in preparation prevention or treatment gastric ulcer functional food.