CN103667118A - Fermentation culture method of Indian bacillus - Google Patents
Fermentation culture method of Indian bacillus Download PDFInfo
- Publication number
- CN103667118A CN103667118A CN201310580668.3A CN201310580668A CN103667118A CN 103667118 A CN103667118 A CN 103667118A CN 201310580668 A CN201310580668 A CN 201310580668A CN 103667118 A CN103667118 A CN 103667118A
- Authority
- CN
- China
- Prior art keywords
- bacillus
- indica
- content
- medium
- fermentation culture
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Withdrawn
Links
Landscapes
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
本发明公开一种印度芽孢杆菌的发酵培养方法，其特征在于：所述的方法按照以下步骤进行：向pH为7-7.5的培养基中接种印度芽孢杆菌，接种量为1-2%，培养温度为25-30℃，装液量为容器的20-40%，摇床转速为150-170r/min，培养时间为48小时，培养基为海水溶液，其中豆粕含量为0.2-0.4g/100ml，麦麸含量为0.2-0.4g/100ml，酵母膏含量为0.1g/100ml。这是一种操作简便，成本低廉，适合于推广和应用的印度芽孢杆菌的发酵培养方法。The invention discloses a method for fermenting and cultivating Bacillus indica, which is characterized in that: the method is carried out according to the following steps: inoculate Bacillus indica into a culture medium with a pH of 7-7.5, the inoculum amount is 1-2%, and culturing The temperature is 25-30°C, the liquid volume is 20-40% of the container, the shaker speed is 150-170r/min, the cultivation time is 48 hours, the medium is seawater solution, and the soybean meal content is 0.2-0.4g/100ml , the content of wheat bran is 0.2-0.4g/100ml, and the content of yeast extract is 0.1g/100ml. This is a method for fermenting and cultivating bacillus indica that is easy to operate, low in cost and suitable for popularization and application.
Description
技术领域 technical field
本发明涉及一种菌的发酵培养方法，特别是一种印度芽孢杆菌的发酵培养方法。 The invention relates to a method for fermentation and cultivation of bacteria, in particular to a method for fermentation and cultivation of Bacillus indica.
背景技术 Background technique
印度芽孢杆菌最早（2004年）发现于印度一淡水水域，在2009年首次发现它存在于海水中，2010年发现其细胞内含有胃液不能破坏的类胡罗卜素，可安全地用做食品添加剂；目前实验室培养印度芽孢杆菌主要利用常规实验用的牛肉膏蛋白胨或是2216E培养基，用这种常规培养基大量培养印度芽孢杆菌的成本较高，不利于印度芽孢杆菌的推广和应用。因此现在业内人员期望找到一种成本低廉的培育印度芽孢杆菌的方法。 Bacillus indica was first discovered (2004) in a freshwater body in India. It was first discovered in seawater in 2009. In 2010, it was found that its cells contained carotenoids that cannot be destroyed by gastric juice, which can be safely used as food additives At present, the laboratory culture of Bacillus indica mainly utilizes beef extract peptone or 2216E medium for routine experiments. The cost of cultivating Bacillus indica in large quantities with this conventional medium is relatively high, which is not conducive to the promotion and application of Bacillus indica. Therefore, people in the industry now expect to find a low-cost method for cultivating Bacillus indica.
发明内容 Contents of the invention
本发明是为了解决现有技术所存在的上述不足，提出一种操作简便，成本低廉，适合于推广和应用的印度芽孢杆菌的发酵培养方法。 The present invention aims to solve the above-mentioned deficiencies in the prior art, and proposes a method for fermentation and cultivation of Bacillus indica that is easy to operate, low in cost and suitable for popularization and application.
本发明的技术解决方案是：一种印度芽孢杆菌的发酵培养方法，其特征在于：所述的方法按照以下步骤进行： The technical solution of the present invention is: a kind of fermentation culture method of bacillus indica, it is characterized in that: described method is carried out according to the following steps:
向pH为7-7.5的培养基中接种印度芽孢杆菌，接种量为1-2%，培养温度为25-30℃，装液量为容器的20-40%，摇床转速为150-170r/min，培养时间为48小时，培养基为海水溶液，其中豆粕含量为0.2-0.4g/100ml，麦麸含量为0.2-0.4g/100ml，酵母膏含量为0.1g/100ml。 Inoculate Bacillus indica into the medium with a pH of 7-7.5, the inoculum amount is 1-2%, the culture temperature is 25-30°C, the liquid volume is 20-40% of the container, and the shaker speed is 150-170r/ min, the culture time is 48 hours, the medium is seawater solution, the content of soybean meal is 0.2-0.4g/100ml, the content of wheat bran is 0.2-0.4g/100ml, and the content of yeast extract is 0.1g/100ml.
本发明同现有技术相比，具有如下优点： Compared with the prior art, the present invention has the following advantages:
本发明所公开的印度芽孢杆菌的发酵培养方法，其操作简单，行之有效。现在业内并没有大规模发酵培养印度芽孢杆菌的方法，传统的实验室培养发放需要使用蛋白胨和牛肉膏作为培养基，其价格较为昂贵，不利于印度芽孢杆菌的大规模、批量化培养，而本发明则公开了一种价格低廉、应用广泛的原料作为培养基，按照合适的比例和条件，可以培养出细胞数量较高、类胡萝卜素含量较高的印度芽孢杆菌，其成本相比于传统的培养基可降低百余倍。因此可以说这种方法具备了多种优点，特别适合于在本领域中推广应用，其市场前景十分广阔。 The method for fermenting and cultivating bacillus indica disclosed by the invention has simple operation and is effective. At present, there is no method for large-scale fermentation and cultivation of Bacillus indica in the industry. The traditional laboratory culture distribution needs to use peptone and beef extract as the medium, which is relatively expensive and is not conducive to large-scale and batch cultivation of Bacillus indica. The invention discloses a cheap and widely used raw material as a culture medium. According to the appropriate ratio and conditions, Bacillus indica can be cultivated with higher cell number and higher carotenoid content. The cost is lower than that of traditional The culture medium can be reduced more than a hundred times. Therefore, it can be said that this method possesses multiple advantages, and is particularly suitable for popularization and application in this field, and its market prospect is very broad.
具体实施方式 Detailed ways
下面将说明本发明实施例的具体实施方式。一种印度芽孢杆菌的发酵培养方法，按照以下步骤进行：首先配置培养基，培养基为以海水为基础的海水溶液，其中豆粕含量为0.2-0.4g/100ml，麦麸含量为0.2-0.4g/100ml，酵母膏含量为0.1g/100ml；配置完成后将培养基的pH值调节至7-7.5，然后向培养基中接种印度芽孢杆菌，接种的印度芽孢杆菌的浓度为108cfu/ml，接种量为1-2%，培养温度为25-30℃，接种后的培养基放入发酵罐等容器中，其装液量为容器的20-40%，将容器放置在摇床上，摇床的转速为150-170r/min，培育48小时，培育出印度芽孢杆菌的数量最高可达1012cfu/ml。 The specific implementation manner of the embodiment of the present invention will be described below. A method for fermenting and cultivating Bacillus indica, which is carried out according to the following steps: first, a culture medium is prepared, and the culture medium is a seawater solution based on seawater, wherein the content of soybean meal is 0.2-0.4g/100ml, and the content of wheat bran is 0.2-0.4g /100ml, the content of yeast extract is 0.1g/100ml; after the configuration is completed, adjust the pH value of the medium to 7-7.5, and then inoculate the medium with Bacillus indica, the concentration of inoculated Bacillus indica is 10 8 cfu/ml , the inoculum amount is 1-2%, the culture temperature is 25-30°C, the culture medium after inoculation is put into a container such as a fermenter, and its liquid volume is 20-40% of the container, and the container is placed on a shaker and shaken The rotation speed of the bed is 150-170r/min, and the cultivation takes 48 hours, and the highest number of Bacillus indica can be cultivated up to 10 12 cfu/ml.
Claims (1)
- The fermentation culture method of 1.Yi Zhong India genus bacillus, is characterized in that: described method is carried out according to following steps:The inoculation of medium India genus bacillus that is 7-7.5 to pH, inoculum size is 1-2%, culture temperature is 25-30 ℃, liquid amount is the 20-40% of container, and shaking speed is 150-170r/min, and incubation time is 48 hours, substratum is sea water solution, wherein soybean meal content is 0.2-0.4g/100ml, and wheat bran content is 0.2-0.4g/100ml, and yeast extract paste content is 0.1g/100ml.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310580668.3A CN103667118A (en) | 2013-11-19 | 2013-11-19 | Fermentation culture method of Indian bacillus |
| CN201410063650.0A CN104099264A (en) | 2013-11-19 | 2014-02-25 | Bacillus indius fermentation culture method |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310580668.3A CN103667118A (en) | 2013-11-19 | 2013-11-19 | Fermentation culture method of Indian bacillus |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103667118A true CN103667118A (en) | 2014-03-26 |
Family
ID=50305899
Family Applications (2)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310580668.3A Withdrawn CN103667118A (en) | 2013-11-19 | 2013-11-19 | Fermentation culture method of Indian bacillus |
| CN201410063650.0A Pending CN104099264A (en) | 2013-11-19 | 2014-02-25 | Bacillus indius fermentation culture method |
Family Applications After (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201410063650.0A Pending CN104099264A (en) | 2013-11-19 | 2014-02-25 | Bacillus indius fermentation culture method |
Country Status (1)
| Country | Link |
|---|---|
| CN (2) | CN103667118A (en) |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB0524873D0 (en) * | 2005-12-06 | 2006-01-11 | New Royal Holloway & Bedford | Bacterial production of carotenoids |
| CN103478044A (en) * | 2013-09-29 | 2014-01-01 | 大连海洋大学 | Method for culturing apostichopusjaponicus juveniles by Indian bacillus |
-
2013
- 2013-11-19 CN CN201310580668.3A patent/CN103667118A/en not_active Withdrawn
-
2014
- 2014-02-25 CN CN201410063650.0A patent/CN104099264A/en active Pending
Also Published As
| Publication number | Publication date |
|---|---|
| CN104099264A (en) | 2014-10-15 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103535511B (en) | A kind of high temperature dregs of beans that ferments produces the preparation method of the rich prebiotics feed of rich peptide | |
| CN102260729A (en) | Bioflocculant fermentation method with mycelium pellet as vector | |
| CN109234331A (en) | A kind of Ganoderma lucidum submerged fermentation is naturally cultivated and tunning complete utilization method | |
| CN101843328B (en) | Production method of corn starch sugar residue rice koji and red rice fermented soy sauce | |
| CN105039484B (en) | It is a kind of to utilize ocean rhodotorula High Yield of Carotenoid and copper fermentation culture method | |
| CN102334702A (en) | Production method for laver protein and polysaccharide nutrient powder | |
| CN104087624A (en) | Method for producing citric acid by continuous fermentation of Aspergillus niger | |
| CN106047740B (en) | A high-yielding strain of L-alanine | |
| CN104630167A (en) | Method for producing low-temperature glucose oxidase by fermentation of marine microorganisms | |
| CN104163675A (en) | Culture medium suitable for cordyceps militaris liquid fermentation | |
| CN103130550B (en) | Culture medium and culture method of male agaric mycelium | |
| CN106479932A (en) | A kind of Lactobacillus pentosus producing gamma aminobutyric acid | |
| CN106497906A (en) | A kind of preparation method of cigarette chamomile extract | |
| CN103740675B (en) | A kind of production method of cellulase | |
| CN105238836A (en) | Preparation method and application of hydrolyzed vegetable protein | |
| CN106434779A (en) | Application of Lactobacillus pentosus SS6 in producing γ-aminobutyric acid | |
| CN103667118A (en) | Fermentation culture method of Indian bacillus | |
| CN103725728B (en) | The preparation method of a kind of Bacillamide compound and Bacillamide precursor | |
| CN102793124A (en) | Method for increasing gamma-aminobutyric acid content and improving fermentation quality of fermented chili product | |
| CN112280812B (en) | Method for improving fermentation yield of aureomycin A and ratio of aureomycin A to aureomycin B | |
| CN110218687B (en) | Preparation method of novel red yeast waste residue microecological preparation | |
| CN110592047B (en) | Novel method for producing feruloyl esterase by fermenting straws with Verbena pyricularis and application | |
| CN103497912B (en) | A strain of Halomonas mural degrading biogenic amines and its application | |
| CN101851588A (en) | A culture method for improving the heavy metal cadmium adsorption capacity of ruckeri yeast | |
| CN102578389A (en) | Method for producing high-protein feed by using guanosine fermentation waste liquor |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| PB01 | Publication | ||
| PB01 | Publication | ||
| C04 | Withdrawal of patent application after publication (patent law 2001) | ||
| WW01 | Invention patent application withdrawn after publication |
Application publication date: 20140326 |