CN103664333B - Composition containing 5-ALA and mycose and leaf fertilizer prepared by using composition - Google Patents

Abstract

The invention relates to a composition containing 5-ALA and trehalose and the foliage fertilizer prepared therefrom. A composition for preparing foliage fertilizer, which contains 2000-4000 parts by weight of trehalose and 0.5-1 part by weight of 5-ALA. A 5-ALA trehalose compound foliar fertilizer, wherein the concentration of trehalose is 2g/L-4g/L, and the concentration of 5-ALA is 0.5mg/L-1mg/L. In the invention, 5-ALA and trehalose are compounded at a specific concentration, and supplemented with a variety of nutritional elements with specific concentrations to make a compound foliage fertilizer. The two can work synergistically. Compared with 5-ALA and trehalose alone, they can significantly increase the chlorophyll content in leaves and promote the photosynthesis of various crops. Especially under adversity, they can maintain cell stability and reduce membrane lipid peroxidation. and other physiological pathways, which significantly enhance the stress resistance of crops, thereby greatly promoting the robust growth of plants and improving crop yield and quality.

Description

A composition containing 5-ALA and trehalose and the foliar fertilizer prepared therefrom

technical field

The invention belongs to the field of fertilizers, in particular to a composition containing 5-ALA and trehalose and a foliage fertilizer prepared therefrom.

Background technique

In recent years, there have been a lot of researches on foliar fertilizers, and there are many types of products, such as "5-ALA photosynthetic power agent" produced by Suzhou Namet Biotechnology Co., Ltd. "Non-toxic fertilizer" and "seaweed foliar fertilizer" produced by Qingdao Mingyue Company have good market sales, have made great contributions to the development of ecological agriculture, and achieved good economic and social benefits. However, in actual production and application, it has been found that these two types of foliar fertilizers have certain defects. Although the spraying effect of 5-ALA foliar fertilizers is obvious, the plant leaves are under adversity stress such as low temperature, high temperature, and salt stress. Insufficient absorption by the leaf surface due to damage, etc., and even damage spots on the leaf surface of crops at high concentrations, so the desired effect cannot be achieved. Although the seaweed foliar fertilizer contains a variety of plant growth substances, trehalose and some nutrients, the spraying effect is better, but compared with 5-ALA foliar fertilizer, it can promote the photosynthesis of plant leaves and increase the accumulation of dry matter in plants. relatively poor. To sum up, although the above two types of foliar fertilizers have better effects than other common foliar fertilizers, they both have certain limitations and cannot be maximized by plants to improve plant growth and fruit quality.

5-ALA is the precursor substance of plant chlorophyll synthesis, which can promote the synthesis of chlorophyll, improve plant photosynthesis, and also have a very obvious effect on improving plant resistance to low temperature, salt resistance, drought and other stress resistance. Moreover, in recent years The application research of 5-ALA photodynamics in clinical medicine is very extensive, and it has a good effect on the treatment of diseases such as skin diseases and cancer. Trehalose is known as the "sugar of life" in the scientific community. It has a miraculous protective effect on organisms, because trehalose can be used on the cell surface under harsh environmental conditions such as high temperature, high cold, high osmotic pressure, and drought. Form a unique protective film, improve the stability of surface cells, effectively protect protein molecules from denaturation and inactivation, thereby maintaining the life process and biological characteristics of living organisms. Many species that exhibit extraordinary stress tolerance to harsh external environments are related to the presence of a large amount of trehalose in their bodies. Therefore, these two substances are beneficial and harmless to plants and human bodies, and belong to green and environmental protection substances. However, when the concentration of trehalose and 5-ALA is too low, the effect is not obvious. When the concentration is too high, it will accelerate the membrane lipid peroxidation of the leaf surface cells and inhibit the synthesis of chlorophyll, thereby reducing the chlorophyll content and affecting its plant growth and development. Crop yield and quality. At present, there is no relevant report on the compound foliar fertilizer of 5-ALA and trehalose.

Contents of the invention

The object of the present invention is to provide a composition containing 5-ALA and trehalose for the above-mentioned defects of the prior art.

Another object of the present invention is to provide a foliar fertilizer prepared from the composition.

The purpose of the present invention can be achieved through the following technical solutions:

A composition for preparing foliage fertilizer, which contains 2000-4000 parts by weight of trehalose and 0.5-1 part by weight of 5-ALA.

The composition preferably further includes the following nutrients: 600-620 parts by weight of KNO ₃ , 110-120 parts by weight of NH ₄ H ₂ PO ₄ , 95-105 parts by weight of MgSO ₄ ·7H ₂ O, 18-105 parts by weight of NaFe-EDTA 23 parts by weight, H ₃ BO ₃ 2.5 to 3.0 parts by weight, MnSO ₄ ·H ₂ O 2.0 to 2.2 parts by weight, ZnSO ₄ 7H ₂ O 0.20 to 0.25 parts by weight, CuSO ₄ ·5H ₂ O 0.05 to 0.10 parts by weight, (NH ₄ ) ₆ Mo ₇ O ₂₄ ·4H ₂ O 0.01 to 0.04 parts by weight.

The nutrients in the composition are further preferably used in the following amounts: 607 parts by weight of KNO ₃ , 115 parts by weight of NH ₄ H ₂ PO ₄ , 100 parts by weight of MgSO ₄ ·7H ₂ O, 20 parts by weight of NaFe-EDTA, H ₃ BO ₃ 2.86 parts by weight, MnSO ₄ ·H ₂ O 2.13 parts by weight, ZnSO ₄ ·7H ₂ O 0.22 parts by weight, CuSO ₄ ·5H ₂ O 0.08 parts by weight, (NH ₄ ) ₆ Mo ₇ O ₂₄ ·4H ₂ O 0.02 parts by weight .

The application of the composition of the invention in the preparation of foliage fertilizer.

A 5-ALA trehalose compound foliar fertilizer, containing water and the composition of the present invention, before spraying, the composition is dissolved in the water to make trehalose concentration of 2g/L～ 4g/L, the concentration of 5-ALA is 0.5mg/L～1mg/L solution.

As a preferred form of the present invention, the 5-ALA trehalose compound foliar fertilizer is dissolved in the water before spraying to make trehalose 2g/L～4g/L, 5-ALA 0.5mg/L～1mg/L, KNO ₃ 600～620mg/L, NH ₄ H ₂ PO ₄ 110～120mg/L, MgSO ₄ 7H ₂ O 95～105mg/L, NaFe-EDTA 18～23mg/L, H ₃ BO ₃ 2.5～3.0mg/L, MnSO ₄ ·H ₂ O 2.0～2.2mg/L, ZnSO ₄ ·7H ₂ O 0.20～0.25mg/L, CuSO ₄ ·5H ₂ O 0.05～0.10mg/L, (NH ₄ ) ₆ Mo ₇ O ₂₄ ·4H ₂ O 0.01-0.04 mg/L solution.

As the most preferred form of the present invention, the 5-ALA trehalose compound foliar fertilizer is dissolved in the water before spraying to prepare trehalose 4g/L, 5-ALA 1mg/L, KNO ₃ 607mg/L, NH ₄ H ₂ PO ₄ 115mg/L, MgSO ₄ ·7H ₂ O100mg/L, NaFe-EDTA 20mg/L, H ₃ BO ₃ 2.86mg/L, MnSO ₄ ·H ₂ O 2.13mg/L , ZnSO ₄ ·7H ₂ O 0.22 mg/L, CuSO ₄ ·5H ₂ O 0.08 mg/L, (NH ₄ ) ₆ Mo ₇ O ₂₄ ·4H ₂ O 0.02 mg/L solution.

The foliage fertilizer of the present invention is recommended to be sprayed on the leaves in the morning or evening.

The preparation method of described composition comprises the following steps:

(1) Take KNO ₃ , NH ₄ H ₂ PO ₄ and MgSO ₄ 7H ₂ O, measure, pulverize and mix to make component A;

(2) After measuring NaFe-EDTA, H ₃ BO ₃ , MnSO ₄ ·H ₂ O, ZnSO ₄ ·7H ₂ O, CuSO ₄ ·5H ₂ O and (NH ₄ ) ₆ Mo ₇ O ₂₄ ·4H ₂ O Separately pulverize and mix with A component to make B component;

(3) Mix 5-ALA and trehalose with component B to make component C;

(4) Crush component C.

On this basis, the foliar fertilizer of the present invention is prepared by diluting the composition with water to a corresponding concentration before spraying. Beneficial effect:

In the present invention, 5-ALA and trehalose are compounded in a specific ratio, supplemented with a variety of specific concentrations of nutrients to make a composition for preparing compound foliage fertilizer, and on this basis, the combination is further determined The optimum amount of each component in the plant in the foliar fertilizer. The experiment found that when the concentration of trehalose is 2g/L-4g/L, and the concentration of 5-ALA is 0.5mg/L-1mg/L, the two can work synergistically, which can significantly improve compared with using 5-ALA and trehalose alone. The chlorophyll content in leaves can promote the photosynthesis of various crops, especially under adversity, it can significantly enhance the stress resistance of crops by maintaining cell stability, reducing membrane lipid peroxidation and other physiological pathways, thereby greatly promoting the robust growth of plants , improve crop yield and quality.

Detailed ways

Example 1

Step 1: Take 9.105g of KNO ₃ , 1.725g of NH ₄ H ₂ PO ₄ and 1.5g of MgSO ₄ ·7H ₂ O, measure, pulverize and mix to make component A.

Step 2: Take NaFe-EDTA 0.3g, H ₃ BO ₃ 0.0429g, MnSO ₄ ·H ₂ O 0.03195g, ZnSO ₄ ·7H ₂ O 0.0033g, CuSO ₄ ·5H ₂ O 0.0012g and (NH ₄ ) ₆ Mo ₇ O ₂₄ ·4H ₂ O 0.0003g is measured and pulverized separately and mixed with component A to make component B.

Step 3: Mix 0.015g of 5-ALA and 60g of trehalose with component B to make component C.

Step 4: Grinding component C to make a composition for preparing foliage fertilizer.

Step 5: Before spraying, dilute the composition with 15L of water to prepare trehalose 4g/L, 5-ALA 1mg/L, KNO ₃ 607mg/L, NH ₄ H ₂ PO ₄ 115mg/L, MgSO ₄ 7H ₂ O 100mg/L, NaFe-EDTA 20mg/L, H ₃ BO ₃ 2.86mg/L, MnSO ₄ H ₂ O 2.13mg/L, ZnSO ₄ 7H ₂ O 0.22mg/L, CuSO ₄ 5H ₂ O 0.08mg/L, (NH ₄ ) ₆ Mo ₇ O ₂₄ ·4H ₂ O 0.02mg/L foliar fertilizer solution.

In production, foliar spraying is generally carried out in the morning or evening.

Example 2

Cucumber was used as the test material, and seedlings were raised in matrix plug trays. The average culture temperature was day/night: 28°C/19°C. After the cucumber seedlings appeared the third true leaf, the foliar fertilizer of each test group was sprayed in the evening, every 5 Spray once a day, spray 3 times in total, and measure the growth of each treated plant 5 days after the end of the treatment. Each test treatment of the present invention is as described in Table 1. '+' means added nutrients, '-' means no added nutrients. The concentrations of nutrients in each test group were KNO ₃ 607mg/L, NH ₄ H ₂ PO ₄ 115mg/L, MgSO ₄ 7H ₂ O 100mg/L, NaFe-EDTA 20mg/L, H ₃ BO ₃ 2.86 mg/L, MnSO ₄ ·H ₂ O 2.13mg/L, ZnSO ₄ ·7H ₂ O 0.22mg/L, CuSO ₄ ·5H ₂ O 0.08mg/L, (NH ₄ ) ₆ Mo ₇ O ₂₄ ·4H ₂ O 0.02mg/L. The preparation method of each test group foliage fertilizer was the same as that in Example 1, only the content of 5-ALA and trehalose added was different.

Table 1: Test Treatments

Table 2: Effects of each treatment on the growth of cucumber plants

Table 3: Effects of trehalose treatment on cucumber plant growth

Note: This table compares different concentrations of seaweed nutrient solution treatment with the control and trehalose aqueous solution. Different letters in the same column indicate significant differences at the 0.05 level among treatments in the same period.

Table 4: Effects of 5-ALA treatment on cucumber plant growth

Note: This table compares the treatment of different concentrations of 5-ALA nutrient solution with the control and 5-ALA aqueous solution. Different letters in the same column indicate significant differences at the 0.05 level among treatments in the same period.

Table 5: Effects of mixed treatment of trehalose and 5-ALA on growth of cucumber plants

Note: This table compares the better treatment of trehalose mixed with 5-ALA with the control and high-concentration mixed treatment. Different letters in the same column indicate significant differences at the 0.05 level among treatments in the same period. It can be seen from the data in Table 3 that after simply spraying a low-concentration trehalose nutrient solution, the growth of the plants was significantly improved compared with the control and the simple spraying of the nutrient solution, but with the increase of the concentration of trehalose, the growth rate of the plants increased significantly. The treatment with lower concentration of growth showed a trend of first increasing and then decreasing. Among them, the spraying effect of 2-4g/L trehalose nutrient solution was relatively better, and the spraying effect of 4g/L trehalose nutrient solution was the most suitable. The total dry weight of the treated plants increased by 30.46% compared with the control. It can be seen from T5 and T38 that the effect of spraying trehalose nutrient solution is better than that of simply spraying trehalose aqueous solution.

As can be seen from the data in Table 4, after simply spraying low-concentration 5-ALA nutrient solution, the plant growth was significantly improved compared with the control and the simple spraying of nutrient solution, but with the increase of 5-ALA concentration , the growth of each plant with a lower concentration treatment showed a trend of first increasing and then decreasing. Among them, the effect of spraying 1mg/L ALA nutrient solution was relatively the most suitable. The total dry weight of the plants in this treatment increased by 38.12% compared with the control, which was determined by T12 and It can be seen from T40 that the effect of spraying ALA nutrient solution is better than that of simply spraying ALA aqueous solution.

It can be seen from the data in Table 5 that when the nutrient solution was mixed with different proportions of trehalose and ALA and sprayed on cucumber leaves, the growth of plants treated with the low-concentration mixed solution was significantly better than that of the control; As the concentration increases, the spraying effect of lower concentration treatment decreases. Overall, the spraying effect of T24 was the best compared with other treatments, which was extremely significant compared with the control, and the total dry weight of the plants increased by 96.51%; Nutrient solution treatment and 4g/L trehalose and 1mg/L ALA mixed aqueous solution treatment were also significantly improved, and the total dry weight of the plants increased by 50.63%, 42.27% and 9.1% respectively. It shows that after spraying an appropriate proportion of trehalose and 5-ALA mixed nutrient solution, the two have a synergistic effect, which can significantly promote plant growth, especially increase the accumulation of plant dry matter by improving plant photosynthesis, etc. However, it can be concluded from T37 and other treatments that the plant growth will be inhibited when the concentration is too high.

In addition, compared with the control, the chlorophyll content of leaves of plants treated with low-concentration ALA and trehalose nutrient solution increased, but decreased after treatment with high-concentration solution, which may be because low-concentration ALA and trehalose nutrient solution can pass through Maintain cell stability, promote the synthesis of chlorophyll, thereby increasing the chlorophyll content, and the high concentration of ALA and trehalose will accelerate the membrane lipid peroxidation of the leaf surface cells, inhibit the synthesis of chlorophyll, thereby reducing the chlorophyll content.

Claims (6)

1., for the preparation of a composition for foliage fertilizer, it is characterized in that containing trehalose 2000 ~ 4000 weight part and 5-ALA 0.5 ~ 1 weight part.

2. composition according to claim 1, is characterized in that also comprising following nutritive substance: KNO ₃600 ~ 620 weight parts, NH ₄h ₂pO ₄110 ~ 120 weight parts, MgSO ₄7H ₂o 95 ~ 105 weight part, NaFe-EDTA 18 ~ 23 weight part, H ₃bO ₃2.5 ~ 3.0 weight parts, MnSO ₄h ₂o 2.0 ~ 2.2 weight part, ZnSO ₄7H ₂o 0.20 ~ 0.25 weight part, CuSO ₄5H ₂o 0.05 ~ 0.10 weight part, (NH ₄) ₆mo ₇o ₂₄4H ₂o 0.01 ~ 0.04 weight part.

3. composition according to claim 2, is characterized in that also comprising following nutritive substance: KNO ₃607 weight parts, NH ₄h ₂pO ₄115 weight parts, MgSO ₄7H ₂o 100 weight part, NaFe-EDTA 20 weight part, H ₃bO ₃2.86 weight parts, MnSO ₄h ₂o 2.13 weight part, ZnSO ₄7H ₂o 0.22 weight part, CuSO ₄5H ₂o 0.08 weight part, (NH ₄) ₆mo ₇o ₂₄4H ₂o 0.02 weight part.

4. the composition according to any one of claim 1 ~ 3 is preparing the application in foliage fertilizer.

5. a 5-ALA trehalose composite foliage fertilizer, it is characterized in that containing the composition according to any one of water and claim 1 ~ 3, before spraying by described composition dissolves in described water, to make trehalose concentration be 2g/L ~ 4g/L, 5-ALA concentration is the solution of 0.5mg/L ~ 1mg/L.

6. the preparation method of the composition described in Claims 2 or 3, is characterized in that comprising following steps:

(1) KNO is got ₃, NH ₄h ₂pO ₄and MgSO ₄7H ₂o, through metering, is mixed and made into component A after pulverizing respectively;

(2) NaFe-EDTA, H is got ₃bO ₃, MnSO ₄h ₂o, ZnSO ₄7H ₂o, CuSO ₄5H ₂o and (NH ₄) ₆mo ₇o ₂₄4H ₂o pulverizes respectively after metering and makes B component after mixing with component A;

(3) get 5-ALA, trehalose and B component and be mixed and made into C component;

(4) namely the pulverizing of C component is made the described composition for the preparation of foliage fertilizer.