CN103651608B - Pesticide composition containing purslane and aconitum carmichaeli extracts as well as preparation method and application thereof - Google Patents
Pesticide composition containing purslane and aconitum carmichaeli extracts as well as preparation method and application thereof Download PDFInfo
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Abstract
The invention relates to a pesticide composition containing purslane and aconite extracts, a preparation method and application thereof, and belongs to the field of pesticides. The invention aims to solve the technical problem of providing a pesticide composition containing purslane and aconitum carmichaeli extracts, a preparation method and application thereof. The active ingredients of the pesticide composition containing the purslane and aconite extracts are extracted from the following raw medicinal materials in parts by weight: 1 part of purslane and 0.2-4 parts of aconite; the active ingredients can be prepared by mixing and extracting the original medicinal materials of purslane and aconite; or extracting herba Portulacae to obtain herba Portulacae extract, extracting radix Aconiti to obtain radix Aconiti extract, and mixing the two extracts. The pesticide composition containing the purslane and aconitum carmichaeli extracts has an obvious effect of preventing and treating lepidoptera pests, and has positive significance for agricultural production.
Description
Technical field
The present invention relates to the composition pesticide containing kitchen garden and aconite extract and preparation method and application, belong to pesticide field.
Background technology
In recent years, in agriculture and forestry field, due to single drug and not Scientific Usage of Drugs, plant pest has produced pesticide resistance in various degree, and agriculture and forestry produce still with chemical pesticide for mainly to prevent and treat kind, cause the destruction increasingly of environmental pollution and ecotope.Along with the raising of people's living standard and the enhancing of health perception, the manufacture and exploit of biopesticide just progressively receives the concern of agricultural chemicals R&D worker, becomes the focus of agriculture and forestry sustainable development, simultaneously, also be environmental protect, the effective way of minimizing or delaying drug resistance and raising drug effect.Wherein, botanical pesticide has become one of focus of biopesticide research.
Kitchen garden is Portulacaceae annual herb plant.Kitchen garden contains the multiple nutritional components such as protein, fat, carbohydrate, dietary fiber, calcium, phosphorus, iron, copper, carotin, vitamin B1, vitamin B2, niacin, vitamin C, especially vitamin and the mineral matter such as calcium, iron such as vitamin A, vitamin C, vitamin b3.Kitchen garden complete stool be used as medicine there is removing toxic substances, that inhibiting bacteria and diminishing inflammation, diuresis stop dysentery, ease constipation disappears is stagnant, debug, the drug effect such as improving eyesight and suppression uterine hemorrhage; External application can control the disease such as erysipelas, venomous snake bite.
The rhizome of Chinese monkshood is the plant of Ranunculaceae Aconitum, containing multiple alkaloid in the rhizome of Chinese monkshood, as Hypaconitine, mesaconine, aconitine, chuan-wu base A, chuan-wu base B (Ka meter Cha Lin), talaisamine etc.In test, the present inventor finds that kitchen garden and the common extract of the rhizome of Chinese monkshood and purslane extract and aconite extract mixing have good synergistic function, can insecticide be prepared into, have good preventive effect to cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva etc.
Summary of the invention
The technical problem to be solved in the present invention is to provide composition pesticide containing kitchen garden and aconite extract and preparation method and application.
The present invention contains the composition pesticide of kitchen garden and aconite extract, and its active component is extracted by the crude drug of following weight portion and forms: kitchen garden 1 part, the rhizome of Chinese monkshood 0.2 ~ 4 part.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: kitchen garden 1 part, the rhizome of Chinese monkshood 0.5 ~ 2 part; Be preferably again and formed by the crude drug extraction of following weight portion: kitchen garden 1 part, the rhizome of Chinese monkshood 0.5 ~ 1.5 part; Be more preferably and formed by the crude drug extraction of following weight portion: kitchen garden 1 part, the rhizome of Chinese monkshood 1 part.
Wherein, the kitchen garden that the present invention adopts and the rhizome of Chinese monkshood are Chinese herbal medicine, contain the activity of the composition pesticide of kitchen garden and aconite extract in order to improve the present invention, the kitchen garden herb that kitchen garden is preferably dry, the aconite root that the rhizome of Chinese monkshood is preferably dry.
The present invention contains the composition pesticide of kitchen garden and aconite extract, and after its active component can be mixed by crude drug kitchen garden and the rhizome of Chinese monkshood, common extraction forms; Also first can be extracted separately by kitchen garden and obtain purslane extract, the rhizome of Chinese monkshood extracts separately and obtains aconite extract, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by kitchen garden and the rhizome of Chinese monkshood, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing kitchen garden and aconite extract, the present invention contains in the composition pesticide of kitchen garden and aconite extract and also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into any one formulation of applicable agricultural use containing the composition pesticide of kitchen garden and aconite extract by the method for this area routine, for the ease of storing and transport, can make microemulsion, aqua or soluble concentrate.
Further, the aqua be preferably made up of the component of following weight portion: kitchen garden and aconite extract, 10 parts of ethanol, 8 parts of emulsifier, 1601,5 parts of ethylene glycol, 57 parts of water, wherein, kitchen garden and aconite extract are that the crude drug extraction of 20 parts forms.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, kitchen garden is prepared from by the following method with aconite extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
Second technical problem that the present invention will solve is to provide the method that preparation contains the composition pesticide of kitchen garden and aconite extract.
The preparation method that the present invention contains the composition pesticide of kitchen garden and aconite extract is made up of following steps:
A, extraction: take kitchen garden particle and rhizome of Chinese monkshood particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, kitchen garden particle and rhizome of Chinese monkshood particle can adopt commercially available particle, and conventional method pulverizing kitchen garden herb and aconite root also can be adopted to obtain.In order to improve the effect of extraction, simultaneously cost-saving, kitchen garden particle and rhizome of Chinese monkshood particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 10g/ml, is more preferably 1:6g/ml.Namely the kitchen garden of lg and rhizome of Chinese monkshood particle can add 3 ~ 12ml solvent extraction, preferably add 4 ~ 10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform simultaneously, is more preferably the ethanol that volume fraction is 95%.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Reflow's cycle is twice, and each return time is 2.5 hours, and merging filtrate, obtains extract.
The application of the composition pesticide that the present invention also provides the present invention to contain kitchen garden and aconite extract in control crop pest.The present invention contains the composition pesticide of kitchen garden and aconite extract, to lepidoptera pest as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid etc. have good preventive effect.
In the agricultural production of reality, in order to improve composition pesticide containing kitchen garden and aconite extract to the control efficiency of insect pest, the described composition pesticide containing kitchen garden and aconite extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of kitchen garden and aconite extract, sprays on crop blade face.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of kitchen garden and aconite extract, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm.Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3 ~ 5 days.
The present invention is mixed by purslane extract and aconite extract containing the composition pesticide of kitchen garden and aconite extract, or jointly extracted by kitchen garden and the rhizome of Chinese monkshood and form, its preventive effect is far away higher than the extract of single component, particularly extract common after kitchen garden and rhizome of Chinese monkshood mixing, owing to having solubilising, hydrotropy effect between kitchen garden and the rhizome of Chinese monkshood, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Compared with prior art, the present invention has the following advantages containing the composition pesticide of kitchen garden and aconite extract:
1, the present invention has synergistic function containing the composition pesticide of kitchen garden and aconite extract, obvious to the control efficiency of lepidoptera pest.
2, kitchen garden of the present invention and the rhizome of Chinese monkshood mix after jointly extracting or extracting respectively, and the composition pesticide made can improve crop immune system ability while quick-acting tagging, and can significantly improve its vigor.
3, the present invention take Chinese herbal medicine as raw material, thus there is not Pesticide Residue, and insect is difficult to produce resistance; Be used alone compared with chemical source agricultural chemicals, environmentally friendly.
4, when the present invention uses containing the composition pesticide of kitchen garden and aconite extract, valid density is low, with low cost.
Embodiment
The present invention contains the composition pesticide of kitchen garden and aconite extract, and its active component is extracted by the crude drug of following weight portion and forms: kitchen garden 1 part, the rhizome of Chinese monkshood 0.2 ~ 4 part.Wherein, active component is preferably extracted by the crude drug of following weight portion and forms: kitchen garden 1 part, the rhizome of Chinese monkshood 0.5 ~ 2 part; Be preferably again and formed by the crude drug extraction of following weight portion: kitchen garden 1 part, the rhizome of Chinese monkshood 0.5 ~ 1.5 part; Be more preferably and formed by the crude drug extraction of following weight portion: kitchen garden 1 part, the rhizome of Chinese monkshood 1 part.
Wherein, the kitchen garden that the present invention adopts and the rhizome of Chinese monkshood are Chinese herbal medicine, contain the activity of the composition pesticide of kitchen garden and aconite extract in order to improve the present invention, the kitchen garden herb that kitchen garden is preferably dry, the aconite root that the rhizome of Chinese monkshood is preferably dry.
The present invention contains the composition pesticide of kitchen garden and aconite extract, and after its active component can be mixed by crude drug kitchen garden and the rhizome of Chinese monkshood, common extraction forms; Also first can be extracted separately by kitchen garden and obtain purslane extract, the rhizome of Chinese monkshood extracts separately and obtains aconite extract, then is mixed by two kinds of extracts.In order to improve the insecticidal properties of composition pesticide of the present invention, after its active component is preferably and is mixed by kitchen garden and the rhizome of Chinese monkshood, common extraction forms.
Further, in order to improve the stability of the composition pesticide containing kitchen garden and aconite extract, the present invention contains in the composition pesticide of kitchen garden and aconite extract and also comprises auxiliary agent, described auxiliary agent can adopt the usual auxiliaries in agricultural chemicals, as at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
Wherein, emulsifier can adopt at least one in non-ionic surface active agent and anion surfactant, described non-ionic surface active agent can be dibenzyl xenyl APEO, styryl phenyl APEO, styryl phenyl APEO, phenethyl phenyl polyoxyethylene one polyethenoxy ether, OPEO, alkylphenol polyoxyethylene sulfosuccinate, aliphatic acid polyethenoxy ether, fatty alcohol-polyoxyethylene ether, aliphatic amine polyoxyethylene ether, castor oil ethylene oxide adduct and derivative thereof, at least one in polyol fatty acid ester and ethylene oxide adduct etc. thereof, described anion surfactant is at least one in calcium dodecyl benzene sulfonate, alkylphenol polyoxyethylene phosphate, phenethyl phenol polyethenoxy ether phosphate etc., as emulsifier 500,601p, 602p etc., in addition, commercially available emulsifier production can also be used.
Wherein, solvent can adopt at least one in water, ethanol, isopropyl alcohol, n-butanol, ethyl acetate, butyl acetate, methyl-sulfoxide, turpentine oil, solvent naphtha, 1-METHYLPYRROLIDONE, dimethyl formamide etc.
Wherein, antifreeze can be glycerine, propane diols, ethylene glycol, sodium chloride, calcium chloride, sorbierite etc.
Wherein, defoamer is silicone defoaming agent etc.
Wherein, dispersant is at least one in lignosulfonates, PAA and propionamide copolymer, alkylnaphthalene sulfonate, polycarboxylic acid salt's condensation product, methyl sodiosul foaliphatate etc.
Wherein, wetting agent can be alkyl sulfate, alkylbenzenesulfonate, alkylnaphthalene sulfonate, aliphatic alcohol polyethenoxy base ether, alkylphenol-polyethenoxy base ether, sorbitan fatty acid ester etc.
Wherein, stabilizing agent can be 2-expoxy propane, butyl glycidyl ether, isopropyl alcohol, n-butanol, polyethylene glycol etc.
The present invention can be prepared into any one formulation of applicable agricultural use containing the composition pesticide of kitchen garden and aconite extract by the method for this area routine, for the ease of storing and transport, can make microemulsion, aqua or soluble concentrate.
Further, the aqua be preferably made up of the component of following weight portion: kitchen garden and aconite extract, 10 parts of ethanol, 8 parts of emulsifier, 1601,5 parts of ethylene glycol, 57 parts of water, wherein, kitchen garden and aconite extract are that the crude drug extraction of 20 parts forms.
For the ease of storing and transport, extract can be concentrated, obtain concentrate, and then be prepared into various formulation, cycles of concentration can according to needs of production from Row sum-equal matrix, preferably, kitchen garden is prepared from by the following method with aconite extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
The preparation method that the present invention contains the composition pesticide of kitchen garden and aconite extract is made up of following steps:
A, extraction: take kitchen garden particle and rhizome of Chinese monkshood particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
Wherein, kitchen garden particle and rhizome of Chinese monkshood particle can adopt commercially available particle, and conventional method pulverizing kitchen garden herb and aconite root also can be adopted to obtain.In order to improve the effect of extraction, simultaneously cost-saving, kitchen garden particle and rhizome of Chinese monkshood particle cross 60 ~ 100 mesh sieves, preferred mistake 80 mesh sieve.
Further, in order to cost-saving, simultaneously also in order to improve extraction efficiency, the solid-liquid ratio in a step is preferably 1:4 ~ 10g/ml, is more preferably 1:6g/ml.Namely the kitchen garden of lg and rhizome of Chinese monkshood particle can add 3 ~ 12ml solvent extraction, preferably add 4 ~ 10ml, more preferably add 6ml.
Conventional organic solvent all can be used for the present invention, and in order to cost-saving, improve dissolution rate, ensure extraction efficiency, organic solvent is preferably methyl alcohol, ethanol, ethyl acetate, acetone, chloroform simultaneously, is more preferably the ethanol that volume fraction is 95%.
In order to shorten preparation time, accelerate extraction rate, can stir when adding hot reflux, mixing speed is preferably 80 ~ 100 revs/min.
Further, reflux temperature is preferably 75 ~ 85 DEG C, is more preferably 82 DEG C; Reflow's cycle is twice, and each return time is 2.5 hours, and merging filtrate, obtains extract.
The present invention contains the composition pesticide of kitchen garden and aconite extract, to lepidoptera pest as cabbage caterpillar, diamond-back moth, prodenia litura, beet snout moth's larva, tea tree tea geometrid etc. have good preventive effect.
In the agricultural production of reality, in order to improve composition pesticide containing kitchen garden and aconite extract to the control efficiency of insect pest, the described composition pesticide containing kitchen garden and aconite extract is preferably applied to crop pest initial stage of origination; The present invention can use by common method containing the composition pesticide of kitchen garden and aconite extract, sprays on crop blade face.Preferably, when using, the present invention is diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm containing the composition pesticide of kitchen garden and aconite extract, namely the composition pesticide that 1g crude drug is obtained after extracting, when using, be diluted with water to 10kg, then its activity component concentration equals 100ppm.Preferably being diluted to activity component concentration is further that 20 ~ 50ppm sprays in plant blade face, and so that water membrane to appear in blade face, not lower of liquid is advisable; Secondary dispenser is carried out, the preferred continuous administration of a season crop 2 ~ 3 times after being preferable over 3 ~ 5 days.
The present invention is mixed by purslane extract and aconite extract containing the composition pesticide of kitchen garden and aconite extract, or jointly extracted by kitchen garden and the rhizome of Chinese monkshood and form, its preventive effect is far away higher than the extract of single component, particularly extract common after kitchen garden and rhizome of Chinese monkshood mixing, owing to having solubilising, hydrotropy effect between kitchen garden and the rhizome of Chinese monkshood, the composition pesticide insecticidal effect made after common extraction is more remarkable.
Below in conjunction with embodiment, the specific embodiment of the present invention is further described, does not therefore limit the present invention among described scope of embodiments.
Embodiment 1 contains the preparation of the composition pesticide of kitchen garden and aconite extract
A, extraction: take dry kitchen garden herb and aconite root, mixing, pulverizes, and crosses 80 mesh sieves, adds extraction solution, refluxing extraction twice, filters, merging filtrate, concentrated, makes extract weight equal the weight of crude drug, obtains extract mw-01 ~ mw-06; Wherein, each parameter is in table 1;
B, preparation: in extract mw-01 ~ mw-06, add auxiliary agent in table 2, stir, must contain the composition pesticide MW-01 ~ MW-06 of kitchen garden and aconite extract.
The preparation technology parameter of table 1 kitchen garden and aconite extract
Wherein, kitchen garden herb and aconite root are all purchased from Anguo City Zhong Tian prepared slices of Chinese crude drugs Co., Ltd.
Table 2 contains the formula of the composition pesticide of kitchen garden and aconite extract
Embodiment 2 contains the preparation of the composition pesticide of kitchen garden and aconite extract
A, extraction: take dry kitchen garden herb, pulverize, and crosses 80 mesh sieves, add extraction solution, refluxing extraction twice, filters, merging filtrate, and concentrated, make extract weight equal the weight of crude drug, obtain extract d1-01 ~ d1-03, wherein, each parameter is in table 3;
Take dry aconite root, pulverize, cross 80 mesh sieves, add extraction solution, refluxing extraction twice, filter, merging filtrate, concentrated, make extract weight equal the weight of crude drug, obtain extract d2-01 ~ d2-03, wherein, each parameter is in table 4;
B, preparation: take Purslane extract, rhizome of Chinese monkshood extract and auxiliary agent, stirring and evenly mixing, obtain the composition pesticide MW-07 ~ MW-12 containing kitchen garden and aconite extract, wherein, each parameter is in table 5.
Table 3
Table 4
Table 5
Test example 1 the present invention contains the efficiency test of the composition pesticide wild cabbage diamond-back moth of kitchen garden and aconite extract
For clearly putting into field efficacy situation containing the composition pesticide of kitchen garden and aconite extract, inventor has carried out field control effectiveness test to this medicament.
1, test material
1.1 for studying thing and object: wild cabbage diamond-back moth
1.2 reagent agent
Product in embodiment: MW-01 ~ MW-12;
Kitchen garden single dose (D1), rhizome of Chinese monkshood single dose (D2):
Chemical reagent contrast (CK-01): gamma cyhalothrin, purchased from Chengdu Kelilong Biochemical Co., Ltd.;
Clear water contrast (CK-02).
Wherein, the preparation method of kitchen garden single dose is: the d1-03 getting 20g, adds 10g ethanol, 8g emulsifier 1601,5g ethylene glycol, 57g water, stirring and evenly mixing, obtained kitchen garden single dose.
The preparation method of rhizome of Chinese monkshood single dose is: the d2-03 getting 20g, adds 10g ethanol, 8g emulsifier 1601,5g ethylene glycol, 57g water, stirring and evenly mixing, obtained rhizome of Chinese monkshood single dose.
2, experimental scheme and method
This test 14 medicaments, 1 contrast medicament, 1 blank, repeats for three times, Gong48Ge community.Minizone and surrounding all leave isolation strip, plot area 12m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the diamond-back moth quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the diamond-back moth number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
3, result of the test
Within 5 days after dispenser, investigate and record the borer population alive on each blade, calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 6.
Table 6
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| MW-01 | 337 | 72 | 78.64 | 81.85 |
| MW-02 | 347 | 47 | 86.46 | 88.50 |
| MW-03 | 352 | 39 | 88.92 | 90.59 |
| MW-04 | 318 | 41 | 87.11 | 89.05 |
| MW-05 | 386 | 62 | 83.94 | 86.36 |
| MW-06 | 394 | 70 | 82.23 | 84.91 |
| MW-07 | 331 | 96 | 71.00 | 75.37 |
| MW-08 | 358 | 87 | 75.70 | 79.36 |
| MW-09 | 369 | 82 | 77.78 | 81.13 |
Continued 6
| MW-10 | 306 | 71 | 76.80 | 80.29 |
| MW-11 | 370 | 97 | 73.78 | 77.73 |
| MW-12 | 388 | 108 | 72.16 | 76.36 |
| D1 | 316 | 278 | 12.03 | 25.28 |
| D2 | 329 | 241 | 26.75 | 37.78 |
| CK-01 | 343 | 32 | 90.67 | 92.08 |
| CK-02 | 327 | 385 | -17.74 | —— |
As can be seen from Table 6, product of the present invention has good preventive effect to diamond-back moth, and better, visible than the control efficiency of single purslane extract and aconite extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.
Test example 2 the present invention contains the efficiency test of the composition pesticide tea tree tea geometrid of kitchen garden and aconite extract
Product MW-01 ~ MW-12 of the present invention is used for preventing and treating tea tree tea geometrid.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the tea geometrid quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the tea geometrid number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, it the results are shown in Table 7.
Table 7
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| MW-01 | 212 | 54 | 74.53 | 77.60 |
Continued 7
| MW-02 | 245 | 47 | 80.82 | 83.13 |
| MW-03 | 256 | 43 | 83.20 | 85.23 |
| MW-04 | 231 | 42 | 81.82 | 84.01 |
| MW-05 | 255 | 52 | 79.61 | 82.06 |
| MW-06 | 227 | 50 | 77.97 | 80.63 |
| MW-07 | 285 | 103 | 63.86 | 68.21 |
| MW-08 | 294 | 86 | 70.75 | 74.27 |
| MW-09 | 246 | 65 | 73.58 | 76.76 |
| MW-10 | 215 | 61 | 71.63 | 75.04 |
| MW-11 | 256 | 79 | 69.14 | 72.86 |
| MW-12 | 268 | 89 | 66.79 | 70.79 |
| D1 | 235 | 203 | 13.62 | 24.02 |
| D2 | 233 | 182 | 21.89 | 31.29 |
| CK-02 | 263 | 299 | -13.69 | —— |
As can be seen from Table 7, product of the present invention has good preventive effect to tea geometrid, and better, visible than the control efficiency of single purslane extract and aconite extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 3 the present invention contains the efficiency test of the composition pesticide wild cabbage prodenia litura of kitchen garden and aconite extract
Product MW-01 ~ MW-12 of the present invention is used for preventing and treating wild cabbage prodenia litura.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 20m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the prodenia litura quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the prodenia litura number alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 8.
Table 8
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| MW-01 | 321 | 87 | 72.90 | 76.36 |
| MW-02 | 325 | 67 | 79.38 | 82.02 |
| MW-03 | 346 | 63 | 81.79 | 84.12 |
| MW-04 | 334 | 68 | 79.64 | 82.24 |
| MW-05 | 311 | 67 | 78.46 | 81.21 |
| MW-06 | 367 | 86 | 76.57 | 79.56 |
| MW-07 | 385 | 142 | 63.12 | 67.83 |
| MW-08 | 342 | 102 | 70.18 | 73.98 |
| MW-09 | 398 | 111 | 72.11 | 75.67 |
| MW-10 | 377 | 112 | 70.29 | 74.08 |
| MW-11 | 365 | 118 | 67.67 | 71.80 |
| MW-12 | 383 | 128 | 66.58 | 70.85 |
| D1 | 392 | 335 | 14.54 | 25.45 |
| D2 | 357 | 283 | 20.73 | 30.85 |
| CK-02 | 328 | 376 | -14.63 | —— |
As can be seen from Table 8, product of the present invention has good preventive effect to prodenia litura, and better, visible than the control efficiency of single purslane extract and aconite extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Test example 4 the present invention contains the efficiency test of the composition pesticide cabbage pieris of kitchen garden and aconite extract
Product MW-01 ~ MW-12 of the present invention is used for preventing and treating cabbage pieris.This test 12 medicaments, 2 single doses, 1 blank, repeats for three times, Gong45Ge community.Minizone and surrounding all leave isolation strip, plot area 10m
2, community respectively processes randomized arrangement.
Be 50ppm by reagent agent thin up to activity component concentration, spray application to plant blade face, so that water membrane to appear in blade face, not lower of liquid is advisable.
Be fixed the cabbage caterpillar quantity statistics alive of blade before the test and listing mark.Within 5 days after dispenser, investigate and record the dark greyish green borer population alive on each blade.Calculate Revision insect recluced rate and preventive effect, calculate and adopt following formula:
Ta: the individual amount of survival after treatment region control; Tb: the individual amount of survival before treatment region control
Ca: the individual amount of survival after the control of check plot; Cb: the individual amount of survival before the control of check plot
Each group is repeated meter of averaging, and calculate Revision insect recluced rate and preventive effect, its result is as shown in table 9.
Table 9
| Test medicine | Radix before medicine | 5 days borer populations alive after medicine | Revision insect recluced rate (%) | Preventive effect (%) |
| MW-01 | 378 | 75 | 80.16 | 83.74 |
| MW-02 | 324 | 56 | 82.72 | 85.84 |
| MW-03 | 356 | 59 | 83.43 | 86.42 |
| MW-04 | 347 | 59 | 83.00 | 86.07 |
| MW-05 | 351 | 64 | 81.77 | 85.06 |
| MW-06 | 397 | 74 | 81.36 | 84.73 |
| MW-07 | 388 | 123 | 68.30 | 74.02 |
| MW-08 | 358 | 102 | 71.51 | 76.65 |
| MW-09 | 342 | 94 | 72.51 | 77.48 |
| MW-10 | 331 | 93 | 71.90 | 76.98 |
| MW-11 | 379 | 112 | 70.45 | 75.78 |
| MW-12 | 337 | 102 | 69.73 | 75.20 |
| D1 | 326 | 289 | 11.35 | 27.35 |
| D2 | 377 | 301 | 20.16 | 34.57 |
| CK-02 | 345 | 421 | -22.03 | —— |
As can be seen from Table 9, product of the present invention has good preventive effect to cabbage caterpillar, and better, visible than the control efficiency of single purslane extract and aconite extract, has synergistic function between product compositions of the present invention.And the preventive effect of the product adopting common extracting method to make is apparently higher than extracting separately rear blended product, visible common extraction contributes to improving dissolution rate, raising insecticide efficiency.In addition, have no during dispenser and have poisoning to show to crop.
Claims (17)
1. contain the composition pesticide of kitchen garden and aconite extract, it is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: kitchen garden 1 part, the rhizome of Chinese monkshood 0.2 ~ 4 part, its extracting method is: take kitchen garden particle and rhizome of Chinese monkshood particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water; Described organic solvent is methyl alcohol, ethanol, ethyl acetate, acetone or chloroform.
2. the composition pesticide containing kitchen garden and aconite extract according to claim 1, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: kitchen garden 1 part, the rhizome of Chinese monkshood 0.5 ~ 2 part.
3. the composition pesticide containing kitchen garden and aconite extract according to claim 2, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: kitchen garden 1 part, the rhizome of Chinese monkshood 0.5 ~ 1.5 part.
4. the composition pesticide containing kitchen garden and aconite extract according to claim 3, is characterized in that, its active component is extracted by the crude drug of following weight portion and forms: kitchen garden 1 part, the rhizome of Chinese monkshood 1 part.
5. the composition pesticide containing kitchen garden and aconite extract according to any one of Claims 1 to 4, is characterized in that: the described composition pesticide containing kitchen garden and aconite extract is microemulsion, aqua or soluble concentrate.
6. the composition pesticide containing kitchen garden and aconite extract according to any one of Claims 1 to 4, it is characterized in that: described containing in the composition pesticide of kitchen garden and aconite extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
7. the composition pesticide containing kitchen garden and aconite extract according to claim 5, it is characterized in that: described containing in the composition pesticide of kitchen garden and aconite extract also comprises auxiliary agent, and described auxiliary agent is at least one in emulsifier, antifreeze, solvent, defoamer, dispersant, wetting agent, stabilizing agent.
8. the composition pesticide containing kitchen garden and aconite extract according to claim 5, it is characterized in that: described aqua is made up of the component of following weight portion: kitchen garden and aconite extract, 10 parts of ethanol, 8 parts of emulsifier 1601,5 parts of ethylene glycol, 57 parts of water, wherein, kitchen garden and aconite extract are that the crude drug extraction of 20 parts forms.
9. the composition pesticide containing kitchen garden and aconite extract according to claim 7, it is characterized in that: described aqua is made up of the component of following weight portion: kitchen garden and aconite extract, 10 parts of ethanol, 8 parts of emulsifier 1601,5 parts of ethylene glycol, 57 parts of water, wherein, kitchen garden and aconite extract are that the crude drug extraction of 20 parts forms.
10. the composition pesticide containing kitchen garden and aconite extract according to claim 8, is characterized in that: kitchen garden is prepared from by the following method with aconite extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
11. composition pesticides containing kitchen garden and aconite extract according to claim 9, is characterized in that: kitchen garden is prepared from by the following method with aconite extract: crude drug is through extracting and being concentrated into identical with crude drug weight.
The method containing the composition pesticide of kitchen garden and aconite extract described in 12. any one of preparation claim 1 ~ 11, is characterized in that being made up of following steps:
A, extraction: take kitchen garden particle and rhizome of Chinese monkshood particle, mixing, adds solvent by solid-liquid ratio 1:3 ~ 12g/ml, and in 60 ~ 95 DEG C of refluxing extraction, filter, obtain extract, wherein, described solvent is organic solvent or water;
B, preparation: extract is made microemulsion, soluble concentrate or aqua.
13. preparations according to claim 12 contain the method for the composition pesticide of kitchen garden and aconite extract, and it is characterized in that: in described a step, kitchen garden particle and rhizome of Chinese monkshood particle cross 60 ~ 100 mesh sieves; Solid-liquid ratio is 1:4 ~ 10g/ml; Reflux temperature is 75 ~ 85 DEG C; Reflow's cycle is twice, and merging filtrate, obtains extract.
14. preparations according to claim 13 contain the method for the composition pesticide of kitchen garden and aconite extract, and it is characterized in that: in described a step, kitchen garden particle and rhizome of Chinese monkshood particle cross 80 mesh sieves; Solid-liquid ratio is 1:6g/ml; Organic solvent to be volume fraction be 95% ethanol; Reflux temperature is 82 DEG C.
The composition pesticide containing kitchen garden and aconite extract described in 15. any one of claim 1 ~ 11 is preventing and treating the application in crop pest.
16. application of composition pesticide in control crop pest containing kitchen garden and aconite extract according to claim 15, is characterized in that: described insect pest is lepidoptera pest; During application, be diluted with water to the activity component concentration of crude drug weighing scale as 10 ~ 100ppm.
17. application of composition pesticide in control crop pest containing kitchen garden and aconite extract according to claim 16, is characterized in that: during application, are diluted with water to the activity component concentration of crude drug weighing scale as 20 ~ 50ppm.
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| CN104472558A (en) * | 2014-11-17 | 2015-04-01 | 柳州市天姿园艺有限公司 | Kapok seed treatment agent for control of chafer insect attack |
| CN106135311A (en) * | 2015-04-08 | 2016-11-23 | 黄武 | One vegetative insecticide |
| CN108112638B (en) * | 2016-11-30 | 2020-12-01 | 华南农业大学 | Application of purslane extract in preparation of medicine for killing Spodoptera litura |
| CN108849900B (en) * | 2018-07-04 | 2020-05-15 | 华南农业大学 | A kind of high-efficiency insecticide and its preparation method and application |
| CN111685131B (en) * | 2020-06-15 | 2021-06-29 | 山东省农业科学院植物保护研究所 | A medicinal composition for preventing peanut rust by utilizing purslane extract and preparation method thereof |
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| US5391779A (en) * | 1992-07-27 | 1995-02-21 | Rohm And Haas Company | Stable extracts from neem seeds |
| CN101720773A (en) * | 2009-12-29 | 2010-06-09 | 华南农业大学 | Insecticidal composition of tea saponin and rotenone |
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| US5391779A (en) * | 1992-07-27 | 1995-02-21 | Rohm And Haas Company | Stable extracts from neem seeds |
| CN101720773A (en) * | 2009-12-29 | 2010-06-09 | 华南农业大学 | Insecticidal composition of tea saponin and rotenone |
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