CN103651261B - Bioflocculation liquid suspension is utilized to hatch the method for halogen worm - Google Patents
Bioflocculation liquid suspension is utilized to hatch the method for halogen worm Download PDFInfo
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- CN103651261B CN103651261B CN201210354029.0A CN201210354029A CN103651261B CN 103651261 B CN103651261 B CN 103651261B CN 201210354029 A CN201210354029 A CN 201210354029A CN 103651261 B CN103651261 B CN 103651261B
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- China
- Prior art keywords
- artemia
- bioflocculation
- halogen worm
- incubator
- liquid suspension
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- 238000000034 method Methods 0.000 title claims abstract description 13
- 229910052736 halogen Inorganic materials 0.000 title description 38
- 150000002367 halogens Chemical class 0.000 title description 38
- 239000006194 liquid suspension Substances 0.000 title description 23
- 239000013535 sea water Substances 0.000 claims abstract description 19
- 230000012447 hatching Effects 0.000 claims abstract description 15
- 239000000725 suspension Substances 0.000 claims abstract description 11
- 239000012141 concentrate Substances 0.000 claims abstract description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 6
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 6
- 239000001301 oxygen Substances 0.000 claims abstract description 6
- 239000012286 potassium permanganate Substances 0.000 claims abstract description 4
- 238000004659 sterilization and disinfection Methods 0.000 claims abstract description 4
- 241000238582 Artemia Species 0.000 claims abstract 10
- 235000013619 trace mineral Nutrition 0.000 claims abstract 3
- 239000011573 trace mineral Substances 0.000 claims abstract 3
- 230000037396 body weight Effects 0.000 claims abstract 2
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 8
- 229910003208 (NH4)6Mo7O24·4H2O Inorganic materials 0.000 claims 1
- XLYOFNOQVPJJNP-ZSJDYOACSA-N heavy water Substances [2H]O[2H] XLYOFNOQVPJJNP-ZSJDYOACSA-N 0.000 claims 1
- 241000133262 Nauplius Species 0.000 abstract description 4
- 238000005273 aeration Methods 0.000 abstract description 4
- 235000013601 eggs Nutrition 0.000 abstract 1
- 150000003839 salts Chemical class 0.000 abstract 1
- 238000011534 incubation Methods 0.000 description 10
- 238000009360 aquaculture Methods 0.000 description 6
- 244000144974 aquaculture Species 0.000 description 6
- 238000002360 preparation method Methods 0.000 description 4
- 238000006213 oxygenation reaction Methods 0.000 description 3
- 238000004064 recycling Methods 0.000 description 3
- 239000002893 slag Substances 0.000 description 3
- 239000002910 solid waste Substances 0.000 description 3
- 230000000694 effects Effects 0.000 description 2
- 238000011010 flushing procedure Methods 0.000 description 2
- 241000195493 Cryptophyta Species 0.000 description 1
- 238000009395 breeding Methods 0.000 description 1
- 230000001488 breeding effect Effects 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 239000013078 crystal Substances 0.000 description 1
- 230000007613 environmental effect Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 230000001788 irregular Effects 0.000 description 1
- 244000144972 livestock Species 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 244000005700 microbiome Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 238000006396 nitration reaction Methods 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 239000002245 particle Substances 0.000 description 1
- 238000005070 sampling Methods 0.000 description 1
- 239000002689 soil Substances 0.000 description 1
- 239000007787 solid Substances 0.000 description 1
- 235000021122 unsaturated fatty acids Nutrition 0.000 description 1
- 150000004670 unsaturated fatty acids Chemical class 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 238000003911 water pollution Methods 0.000 description 1
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- Farming Of Fish And Shellfish (AREA)
Abstract
利用生物絮凝体悬浮液孵化卤虫的方法，本发明采用生物絮凝体悬浮液与卤虫孵化器，其特征是在TSS浓度为卤虫体重10%的生物絮凝体悬浮液中加入人工海水，将盐度调节为30‰，并按1L加入1mL微量元素浓缩液，pH调节为7；卤虫孵化器用300mg/L高锰酸钾浸泡5min，用海水冲洗进行消毒，孵化器中加入按照前述方法配制的生物絮凝体悬浮液，每升生物絮凝体悬浮液放卤虫卵3g，连续充气，溶解氧为5.28mg/L，当孵出无节幼体后，减少充气量。
Utilize the method for hatching Artemia with biological floc suspension, the present invention adopts biological floc suspension and Artemia incubator, it is characterized in that artificial seawater is added in the biological floc suspension whose TSS concentration is 10% of Artemia body weight, salt Adjust the temperature to 30‰, and add 1mL trace element concentrate according to 1L, and adjust the pH to 7; soak the Artemia incubator with 300mg/L potassium permanganate for 5min, rinse with seawater for disinfection, and add the biological For the floc suspension, put 3g of Artemia eggs per liter of biological floc suspension, continuously inflate, and the dissolved oxygen is 5.28mg/L. After the nauplius hatch, reduce the amount of aeration.
Description
Technical field
The present invention relates to the hatching m of aquaculture field halogen worm.
Background technology
Biological floe by microorganism, algae, protozoa and other chips, organic granular forms, and is a very opening, very loose permeable structure, the activity of highdensity heterotroph and nitration reaction can occur in flco by it simultaneously.Typical flco shape is irregular, has the particle diameter of extensively distribution, containing abundant colloidal solid.Containing abundant nutriment in biological floe, there is the composition such as higher protein content and unsaturated fatty acid EPA, DHA and PHB.At present, based on the aquaculture pattern (Bio-flocAquacultureSystem of bioflocculation, BAS) recycling rate of waterused of breeding water can be improved at lower cost, obviously reduce feed cost, be considered to a kind of innovation pattern with good economic benefits and environmental benefit.For the feature of the non-selective filter food of halogen worm, the floccule body utilizing the residual bait of aquaculture and coproculture to obtain is used for the hatching of halogen worm, both be conducive to ingesting the growth of the aquatic livestock young of halogen worm, and the nutriment in residual bait ight soil can be avoided again directly to enter the waste and water pollution caused in water.Summary of the invention the object of this invention is to provide a kind of method utilizing bioflocculation liquid suspension to hatch halogen worm, realizes the fortification of halogen worm and improves halogen worm incubation rate and recycle solid waste of aquaculture.
Summary of the invention
Technical scheme of the present invention adopts bioflocculation liquid suspension and halogen worm incubator, it is characterized in that in TSS concentration being that halogen polypide weighs in the bioflocculation liquid suspension of 10% and adds artificial seawater, salinity is adjusted to 30 ‰, and add 1mL microelement concentrate by 1L, pH is adjusted to 7, and described microelement concentrate adds 10gMgSO in 1L pure water
47H
2o, 2.2gZnSO
47H
2o, 7.3gCaCl
22H
2o, 2.5gMnCl
24H
2o, 0.5gCoCl
26H
2o, 0.5g(NH
4) 6Mo
7o
244H
2o, 5gFeSO
47H
2o, 0.2gCuSO
47H
2o; Halogen worm incubator 300mg/L potassium permanganate soaks 5min, carry out disinfection with seawater flushing, the bioflocculation liquid suspension according to preceding method preparation is added in incubator, often liter of bioflocculation liquid suspension puts slag oxygenation 3g, continuous charge, dissolved oxygen is 5.28mg/L, after hatching nauplius, reduces aeration quantity.Halogen worm hatching m of the present invention, owing to scientifically utilizing the abundant nutrition material contained in biological floes, proves to achieve significant technique effect by contrast experiment.The halogen worm that the present invention cultivates is when 36h, and incubation rate is 76.42% ± 19.19%, and halogen worm is when 36h, 60h, 84h, and average body length is 555.96 ± 110.90 μm, 885.53 ± 95.49 μm, 1072.35 ± 151.55 μm.And do not add in the seawater incubator of bioflocculation liquid suspension, halogen worm is 66.00% ± 13.00% at the incubation rate of 36h, do not add in the seawater incubation device of bioflocculation liquid suspension, halogen worm is when 36h, 60h, 84h, average body length is 451.83 ± 47.85 μm, 859.82 ± 94.90 μm, 632.32 ± 138.17 μm, the visible method utilizing bioflocculation liquid suspension to hatch halogen worm, not only can improve halogen worm incubation rate to the fortification of halogen worm, and realize solid waste of aquaculture recycling.
Accompanying drawing explanation
Fig. 1 be under different incubation condition halogen worm in the long situation of change of the body of Different periods.
Fig. 2 is the halogen worm microscopic examination figure of 36h bioflocculation liquid suspension hatching.
Fig. 3 is the halogen worm microscopic examination figure of 36h artificial seawater hatching.
Fig. 4 is the halogen worm microscopic examination figure of 60h bioflocculation liquid suspension hatching.
Fig. 5 is the halogen worm microscopic examination figure of 60h artificial seawater hatching.
Fig. 6 is the halogen worm microscopic examination figure of 84h bioflocculation liquid suspension hatching.
Fig. 7 is the halogen worm microscopic examination figure of 84h artificial seawater hatching.
Embodiment
The present invention adopts bioflocculation liquid suspension and halogen worm incubator, and be that halogen polypide weighs in the bioflocculation liquid suspension of 10% and adds artificial seawater in TSS concentration, salinity is adjusted to 30 ‰, and adds 1mL microelement concentrate by 1L, pH is adjusted to 7.The concrete steps of biological floes suspension preparation are the TSS measuring the biological floes that early stage cultivates, according to the TSS concentration determined, calculate the volume required for bioflocculation liquid suspension that preparation 1LTSS concentration weighs 10% for halogen polypide, take out corresponding volume, constant volume is to 1L; Then add artificial seawater, the compound method of artificial seawater adds sea crystal in 1L pure water, and salinity is adjusted to about 30 ‰, and adds 1mL microelement concentrate, pH is adjusted to about 7.Described microelement concentrate adds 10gMgSO in 1L pure water
47H
2o, 2.2gZnSO
47H
2o, 7.3gCaCl
22H
2o, 2.5gMnCl
24H
2o, 0.5gCoCl
26H
2o, 0.5g(NH
4) 6Mo7O
244H
2o, 5gFeSO
47H
2o, 0.2gCuSO
47H
2o.Halogen worm incubator 300mg/L potassium permanganate soaks 5min, carry out disinfection with seawater flushing, the bioflocculation liquid suspension according to preceding method preparation is added in incubator, often liter of bioflocculation liquid suspension puts slag oxygenation 3g, continuous charge, dissolved oxygen is 5.28mg/L, after hatching nauplius, reduces aeration quantity.The present invention is by adding bioflocculation liquid suspension in one group of incubator, another group adds artificial seawater and carries out contrast experiment, every premium on currency puts slag oxygenation 3g, continuous charge, recording the reactor water temperature adding bioflocculation liquid suspension is 27.81 DEG C, salinity is 29.42 ‰, and dissolved oxygen is 5.28mg/L, pH is 8.16; Adding water temperature in the reactor of artificial seawater is 27.56 DEG C, and salinity is 29.72 ‰, and dissolved oxygen is 7.27mg/L, pH is 8.14.After hatching nauplius, reduce aeration quantity; Microscopic examination is carried out in 36h, 60h, 84h sampling of hatching, result is that the halogen worm of this method cultivation is when 36h, incubation rate is 76.42% ± 19.19%, halogen worm is when 36h, 60h, 84h, average body length is 555.96 ± 110.90 μm, 885.53 ± 95.49 μm, 1072.35 ± 151.55 μm.And do not add in the seawater incubator of bioflocculation liquid suspension, halogen worm is 66.00% ± 13.00% at the incubation rate of 36h, do not add in the seawater incubation device of bioflocculation liquid suspension, halogen worm is when 36h, 60h, 84h, average body length is 451.83 ± 47.85 μm, 859.82 ± 94.90 μm, 632.32 ± 138.17 μm.The visible method utilizing bioflocculation liquid suspension to hatch halogen worm, not only can improve halogen worm incubation rate to the fortification of halogen worm, and realize solid waste of aquaculture recycling.
Claims (1)
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| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210354029.0A CN103651261B (en) | 2012-09-21 | 2012-09-21 | Bioflocculation liquid suspension is utilized to hatch the method for halogen worm |
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|---|---|---|---|
| CN201210354029.0A CN103651261B (en) | 2012-09-21 | 2012-09-21 | Bioflocculation liquid suspension is utilized to hatch the method for halogen worm |
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| CN103651261A CN103651261A (en) | 2014-03-26 |
| CN103651261B true CN103651261B (en) | 2016-04-27 |
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| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN105707016A (en) * | 2016-03-08 | 2016-06-29 | 天津科技大学 | Method for artemia culture in high salinity environment |
| CN106386608B (en) * | 2016-10-12 | 2019-04-30 | 上海海洋大学 | A kind of high-efficiency culture method of Litopenaeus vannamei |
| CN109845703A (en) * | 2019-01-14 | 2019-06-07 | 海南海壹水产种苗有限公司 | A kind of separation method of artemia nauplii |
| CN110301385A (en) * | 2019-08-16 | 2019-10-08 | 渤海水产股份有限公司 | A method of cultivation fairy shrimp |
| CN114698597B (en) * | 2021-12-30 | 2023-04-25 | 上海海洋大学 | Method for recycling aquaculture solid waste |
Citations (7)
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|---|---|---|---|---|
| EP1195088A1 (en) * | 2000-10-05 | 2002-04-10 | Inve Technologies N.V. | Method for producing free swimming Artemia nauplii and packaged cysts for use in that method |
| EP1433389A1 (en) * | 2001-10-02 | 2004-06-30 | Morinaga Milk Industry Co., Ltd. | Acidic drinks containing tofu puree |
| CN1807272A (en) * | 2006-01-25 | 2006-07-26 | 华中师范大学 | Algae bioflocculation agent preparation method |
| EP1767101A1 (en) * | 2005-09-26 | 2007-03-28 | Inve Technologies N.V. | Method to enhance hatching percentage of Artemia diapauzing cysts |
| CN101081015A (en) * | 2006-08-18 | 2007-12-05 | 天津市佳音生物饲料有限公司 | Method for producing artemia nauplius from artemia sporangiocyst |
| CN102027891A (en) * | 2009-09-29 | 2011-04-27 | 天津市佳音生物饲料有限公司 | Methods of Improving the Hatch Rate of Artemia Eggs |
| CN102524196A (en) * | 2012-03-23 | 2012-07-04 | 天津海友佳音生物科技股份有限公司 | Methods of Improving the Hatch Rate of Artemia Eggs |
Family Cites Families (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5023183A (en) * | 1987-11-20 | 1991-06-11 | Biosys Corporation | Mass production in liquid culture of insect-killing nematodes |
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- 2012-09-21 CN CN201210354029.0A patent/CN103651261B/en not_active Expired - Fee Related
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| EP1195088A1 (en) * | 2000-10-05 | 2002-04-10 | Inve Technologies N.V. | Method for producing free swimming Artemia nauplii and packaged cysts for use in that method |
| EP1433389A1 (en) * | 2001-10-02 | 2004-06-30 | Morinaga Milk Industry Co., Ltd. | Acidic drinks containing tofu puree |
| EP1767101A1 (en) * | 2005-09-26 | 2007-03-28 | Inve Technologies N.V. | Method to enhance hatching percentage of Artemia diapauzing cysts |
| CN1807272A (en) * | 2006-01-25 | 2006-07-26 | 华中师范大学 | Algae bioflocculation agent preparation method |
| CN101081015A (en) * | 2006-08-18 | 2007-12-05 | 天津市佳音生物饲料有限公司 | Method for producing artemia nauplius from artemia sporangiocyst |
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