CN103639138B - Full automatic high efficiency ELIAS microplate washer - Google Patents
Full automatic high efficiency ELIAS microplate washer Download PDFInfo
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- CN103639138B CN103639138B CN201310651553.9A CN201310651553A CN103639138B CN 103639138 B CN103639138 B CN 103639138B CN 201310651553 A CN201310651553 A CN 201310651553A CN 103639138 B CN103639138 B CN 103639138B
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B3/00—Cleaning by methods involving the use or presence of liquid or steam
- B08B3/02—Cleaning by the force of jets or sprays
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- B—PERFORMING OPERATIONS; TRANSPORTING
- B08—CLEANING
- B08B—CLEANING IN GENERAL; PREVENTION OF FOULING IN GENERAL
- B08B13/00—Accessories or details of general applicability for machines or apparatus for cleaning
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- F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
- F26—DRYING
- F26B—DRYING SOLID MATERIALS OR OBJECTS BY REMOVING LIQUID THEREFROM
- F26B5/00—Drying solid materials or objects by processes not involving the application of heat
- F26B5/08—Drying solid materials or objects by processes not involving the application of heat by centrifugal treatment
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Abstract
Description
(一)技术领域:(1) Technical field:
本发明涉及医疗设备,具体为一种全自动高效酶标洗板机。The invention relates to medical equipment, in particular to a fully automatic high-efficiency enzyme label washer.
(二)背景技术:(2) Background technology:
目前酶标洗板机已经广泛应用于医院、血站、卫生防疫站、研究室以及试剂厂等,专门用来清洗酶标板。At present, the enzyme label washing machine has been widely used in hospitals, blood banks, health and epidemic prevention stations, research laboratories and reagent factories, etc., and is specially used to clean enzyme label plates.
酶标洗板机洗涤的目的是用清洗喷头将固相载体上形成的抗原抗体复合物与液体中的其他多余的游离反应物质分开,即洗涤除去未结合的抗原及杂质,经洗涤后,固相载体上只留下特异性抗体,其他免疫球蛋白及血清中的杂质由于不能与固相抗原结合,在洗涤过程中将被洗去,洗板的好坏直接影响到实验结果的成败。The purpose of washing with the enzyme label washing machine is to use the cleaning nozzle to separate the antigen-antibody complex formed on the solid-phase carrier from other redundant free reaction substances in the liquid, that is, to remove unbound antigens and impurities by washing. After washing, the solid-phase Only the specific antibody is left on the phase carrier, and other immunoglobulins and impurities in the serum will be washed away during the washing process because they cannot combine with the solid-phase antigen. The quality of the plate washing directly affects the success or failure of the experimental results.
目前,市面上酶标洗板机的清洗和脱水大多都分两个设备来完成,也有少数的清洗和脱水是一体的,但是效果不是太好,效率不是太高,还容易产生交叉感染。At present, most of the washing and dehydration of enzyme label washers on the market are completed by two devices, and there are also a few washing and dehydration that are integrated, but the effect is not very good, the efficiency is not too high, and cross infection is prone to occur.
(三)发明内容:(3) Contents of the invention:
为此,本发明提出了一种全自动高效酶标洗板机,所要解决的技术问题是提高效率和避免交叉感染。For this reason, the present invention proposes a fully automatic high-efficiency enzyme label washer, and the technical problem to be solved is to improve efficiency and avoid cross-infection.
能够解决上述技术问题的全自动高效酶标洗板机,包括设于桶体内的提升装置、清洗装置、酶标板支撑移送装置和脱水装置,与现有技术不同的是所述提升装置包括吊板和升降机构,所述吊板设于桶体的桶口,所述升降机构安装于吊板底部;所述清洗装置设于升降机构下方,包括清洗板和由喷液头和吸液头集成的清洗头,所述清洗板安装于升降机构上,所述清洗头以列阵方式设于清洗板底部;所述酶标板支撑移送装置设于清洗头下方,包括安放酶标板的支撑架和将酶标板从支撑架一侧推离口送出的翻转推送架,所述翻转推送架设于酶标板下方的支撑架底部;所述脱水装置包括护桶、脱水架、旋转架以及旋转架传动机构,所述护桶竖直设于桶体底部,支撑架设于护桶的桶口,所述旋转架与护桶同轴套装,至少两个脱水架于旋转架上对称设置,脱水架的外侧板面上开设对应于酶标板各排微孔的脱水槽口,脱水架的内侧板面上部开设对应于支撑架推离口的酶标板进入口,所述旋转架传动机构的传动齿轮设于护桶内,旋转架的内圈齿轮与传动齿轮啮合,所述传动齿轮的动力机构为脱水电机。A fully automatic high-efficiency enzyme label washing machine capable of solving the above technical problems, including a lifting device, a cleaning device, a support and transfer device for the enzyme label plate, and a dehydration device arranged in the barrel. The difference from the prior art is that the lifting device includes a hanging Plate and lifting mechanism, the hanging plate is arranged at the barrel mouth of the barrel body, the lifting mechanism is installed at the bottom of the hanging plate; the cleaning device is arranged under the lifting mechanism, including the cleaning plate and integrated by the spray head and the liquid suction head The cleaning head, the cleaning plate is installed on the lifting mechanism, and the cleaning head is arranged at the bottom of the cleaning plate in an array; the support and transfer device for the microplate plate is arranged under the cleaning head, including a support frame for placing the microplate plate And the reverse push rack that pushes the microplate plate out from the opening on one side of the support frame, and the reverse push frame is installed at the bottom of the support frame below the microplate plate; the dehydration device includes a protective bucket, a dehydration frame, a rotating frame and a rotating frame The transmission mechanism, the barrel is vertically arranged at the bottom of the barrel, and the support is set on the mouth of the barrel. The rotating frame and the barrel are coaxially set, and at least two dehydrating frames are symmetrically arranged on the rotating frame. The dehydration notch corresponding to each row of micropores of the microplate is provided on the outer plate, and the microplate entry port corresponding to the push-off opening of the support frame is provided on the upper part of the inner plate of the dehydration frame. The transmission gear of the rotating frame transmission mechanism Set in the protective bucket, the inner ring gear of the rotating frame meshes with the transmission gear, and the power mechanism of the transmission gear is a dehydration motor.
所述升降机构的一种结构包括丝杆、移动板和提升架,所述丝杆于吊板底部水平设置,丝杆中点的左、右两段分别为旋向相反的螺纹,所述移动板于丝杆中点的左、右水平设置并垂直于丝杆,移动板中心固定的螺母与丝杆对应段的螺纹旋合,各移动板的两端滑动配合于两侧与丝杆方向一致的水平槽内;所述提升架分别设于吊板两侧,每侧提升架包括两等长的长连杆和两等长的短连杆,两长连杆交叉铰连,两长连杆的上端分别与同侧面的左、右移动板的端头铰连,所述两短连杆对应于长连杆的下端左、右对称设置,短连杆的上端与对应长连杆的下端铰连,短连杆的下端分别与清洗板的侧面铰连,在整个运动过程中,短连杆下端的间距都小于短连杆上端的间距;所述升降机构的动力机构为升降电机,所述升降电机的输出轴连接丝杆的一端。A structure of the lifting mechanism includes a screw rod, a moving plate and a lifting frame. The screw rod is arranged horizontally at the bottom of the hanging plate. The plates are set horizontally on the left and right of the midpoint of the screw rod and are perpendicular to the screw rod. The nut fixed in the center of the moving plate is screwed with the thread of the corresponding section of the screw rod. in the horizontal groove; the lifting frames are respectively arranged on both sides of the hanging plate, and each side lifting frame includes two equal-length long connecting rods and two equal-length short connecting rods, the two long connecting rods are cross-hinged, and the two long connecting rods The upper end of the upper end is respectively hinged with the ends of the left and right moving plates on the same side, and the two short connecting rods are arranged symmetrically to the left and right of the lower end of the long connecting rod, and the upper end of the short connecting rod is hinged with the lower end of the corresponding long connecting rod. The lower ends of the short connecting rods are respectively hinged with the side of the cleaning plate, and the distance between the lower ends of the short connecting rods is less than the distance between the upper ends of the short connecting rods during the whole movement process; the power mechanism of the lifting mechanism is a lifting motor, and the The output shaft of the lifting motor is connected to one end of the screw mandrel.
所述清洗板的一种结构包括盖板、吸液板和喷液板,所述盖板、吸液板和喷液板三者自上而下贴合固定为一体,与盖板贴合的吸液板的板面上开设多列相互平行的吸液槽,所述吸液槽上均匀间隔开设多个贯通吸液板的吸液孔,与吸液板贴合的喷液板的板面上开设有与吸液槽列数相等、方向一致的喷液槽,对应于各吸液孔位置,于所述喷液槽上开设有贯通喷液板的喷液孔,所述喷液头的上部于喷液板端面上安装于喷液孔内,所述吸液头贯通喷液头中心,吸液头的上端安装于吸液孔内,吸液头的下端伸出喷液头外2mm~4mm,喷液头内部开设有喷射孔,所述喷射孔的喷射方向与喷液头中心线的夹角为5°~15°;各吸液槽的两端分别连通对应设置的吸液机构,各喷液槽的两端分别连通对应设置的喷液机构。A structure of the cleaning plate includes a cover plate, a liquid absorbing plate and a liquid spraying plate, and the cover plate, the liquid absorbing plate and the liquid spraying plate are bonded and fixed together from top to bottom. A plurality of rows of parallel liquid suction grooves are provided on the surface of the liquid suction plate, and a plurality of liquid suction holes penetrating the liquid suction plate are evenly spaced on the liquid suction groove, and the surface of the liquid spray plate that is bonded to the liquid suction plate There are liquid spray tanks with the same number of rows as the liquid suction tanks and the same direction. Corresponding to the position of each liquid suction hole, there are liquid spray holes through the liquid spray plate on the liquid spray tank. The liquid spray head The upper part is installed in the liquid spray hole on the end surface of the liquid spray plate. The liquid suction head runs through the center of the liquid spray head. 4mm, there is a spray hole inside the liquid spray head, the angle between the spray direction of the spray hole and the center line of the liquid spray head is 5°~15°; the two ends of each liquid suction groove are respectively connected to the corresponding liquid suction mechanism, The two ends of each liquid spray tank are respectively connected with the correspondingly provided liquid spray mechanism.
所述喷液机构的一种结构包括喷液管、喷液瓶和充气泵,所述喷液管连通喷液瓶和喷液槽的端口,所述喷液瓶通过气管连通充气泵;所述吸液机构的一种结构包括吸液管、吸液瓶和吸气泵,所述吸液管连通吸液槽的端头与吸液瓶,所述吸液瓶通过气管连通吸气泵。A structure of the liquid spray mechanism includes a liquid spray pipe, a liquid spray bottle and an air pump, the liquid spray pipe communicates with the ports of the liquid spray bottle and the liquid spray tank, and the liquid spray bottle communicates with the air pump through a gas pipe; One structure of the liquid suction mechanism includes a suction pipe, a liquid suction bottle and a suction pump, the liquid suction pipe communicates with the end of the liquid suction tank and the liquid suction bottle, and the liquid suction bottle communicates with the suction pump through a trachea.
还设有集液装置,所述集液装置包括集液管和集液瓶,所述集液管连通桶体底部的排液口和集液瓶。A liquid collection device is also provided, and the liquid collection device includes a liquid collection pipe and a liquid collection bottle, and the liquid collection pipe communicates with the liquid discharge port at the bottom of the barrel and the liquid collection bottle.
常规上将所述清洗头的列阵设为12×8,即12列(排)清洗头,每列(排)中8个清洗头。Conventionally, the array of cleaning heads is set to 12×8, that is, there are 12 columns (rows) of cleaning heads, and there are 8 cleaning heads in each column (row).
本发明的有益效果:Beneficial effects of the present invention:
本发明全自动高效酶标洗板机采用清洗和脱水一体化设计方案,大大提高了工作效率,较好的杜绝了交叉感染。The fully automatic high-efficiency enzyme label washing machine of the present invention adopts an integrated design scheme of cleaning and dehydration, which greatly improves the working efficiency and better prevents cross-infection.
(四)附图说明:(4) Description of the drawings:
图1为本发明一种实施方式的结构示意图。Fig. 1 is a schematic structural diagram of an embodiment of the present invention.
图2图1实施方式中桶体内部各装置的轴测图。Fig. 2 is the isometric view of each device inside the bucket body in the embodiment of Fig. 1 .
图3为图1实施方式中酶标板支撑移送装置的工作示意图。Fig. 3 is a working schematic diagram of the microplate plate supporting and transferring device in the embodiment shown in Fig. 1 .
图4为图1、图2实施方式中清洗板的结构示意图。Fig. 4 is a schematic structural view of the cleaning plate in the embodiment shown in Fig. 1 and Fig. 2 .
图5为图4中喷液板的结构示意图。FIG. 5 is a schematic structural view of the liquid spray plate in FIG. 4 .
图6为图4中吸液板的结构示意图。Fig. 6 is a schematic structural view of the liquid suction plate in Fig. 4 .
图7(a)为图4中喷液头的结构示意图。FIG. 7( a ) is a schematic diagram of the structure of the liquid ejection head in FIG. 4 .
图7(b)为图7(a)中的A-A剖示图。Fig. 7(b) is a sectional view of A-A in Fig. 7(a).
图号标识:1、桶体;2、吊板;3、清洗板;3-1、盖板;3-2、吸液板;3-2-1、吸液槽;3-2-2、吸液孔;3-3、喷液板;3-3-1、喷液槽;3-3-2、喷液孔;4、清洗头;4-1、喷液头;4-1-1、喷射孔;4-2、吸液头;5、支撑架;6、翻转推送架;7、护桶;8、脱水架;9、旋转架;10、传动齿轮;11、脱水电机;12、丝杆;13、移动板;14、长连杆;15、短连杆;16、升降电机;17、喷液管;18、喷液瓶;19、集液管;20、集液瓶;21、酶标板;22、外壳;23、吸液管;24、吸液瓶。Drawing number identification: 1, barrel body; 2, hanging plate; 3, cleaning plate; 3-1, cover plate; 3-2, liquid suction plate; 3-2-1, liquid suction tank; 3-2-2, Liquid suction hole; 3-3, liquid spray plate; 3-3-1, liquid spray tank; 3-3-2, liquid spray hole; 4, cleaning head; 4-1, liquid spray head; 4-1-1 , injection hole; 4-2, liquid suction head; 5, support frame; 6, flip push frame; 7, protective bucket; 8, dehydration frame; Screw rod; 13, moving plate; 14, long connecting rod; 15, short connecting rod; 16, lifting motor; 17, liquid spray pipe; 18, spray liquid bottle; 19, liquid collecting pipe; 20, liquid collecting bottle; 21 , microplate; 22, shell; 23, pipette; 24, pipette bottle.
(五)具体实施方式:(5) Specific implementation methods:
下面结合附图所示实施方式对本发明的技术方案作进一步说明。The technical solutions of the present invention will be further described below in conjunction with the embodiments shown in the accompanying drawings.
本发明全自动高效酶标洗板机主要由桶体1、提升装置、清洗装置、酶标板支撑移送装置和脱水装置组成。The fully automatic high-efficiency enzyme label washing machine of the present invention is mainly composed of a barrel body 1, a lifting device, a cleaning device, an enzyme label plate support transfer device and a dehydration device.
所述桶体1于外壳22上安装,所述提升装置、清洗装置、酶标板支撑移送装置和脱水装置自上而下设于桶体1内。The barrel body 1 is installed on the shell 22, and the lifting device, cleaning device, microplate support transfer device and dehydration device are arranged in the barrel body 1 from top to bottom.
如图1、图2所示,所述提升装置包括方形吊板2和升降机构,所述吊板2设于桶体1的桶口上,所述升降机构安装于吊板2底部。As shown in Figures 1 and 2, the lifting device includes a square hanging plate 2 and a lifting mechanism, the hanging plate 2 is arranged on the bung of the barrel body 1, and the lifting mechanism is installed on the bottom of the hanging plate 2.
所述升降机构由丝杆12、移动板13、升降电机16和提升架组成。Described lifting mechanism is made up of screw mandrel 12, moving plate 13, lifting motor 16 and lifting frame.
所述丝杆12沿吊板2的长度方向水平设置于吊板2的底部,丝杆12的两端由吊板2上固设的左、右轴承座支撑,丝杆12中点的左、右两段分别为旋向相反的螺纹,所述升降电机16设于吊板2底部,升降电机16的水平输出轴连接丝杆12的一端;所述移动板13以丝杆12的中点左、右水平设置且与丝杆12垂直,两移动板13中心固连的螺母分别与丝杆12的左、右两段螺纹旋合,各移动板13的两端销轴滑动配合于两侧侧板(建立于吊板2上)上开设的与丝杆12方向一致的水平槽内。Described screw mandrel 12 is arranged on the bottom of hanging plate 2 horizontally along the length direction of hanging plate 2, and the two ends of screw mandrel 12 are supported by the left and right bearing seats that are fixed on the hanging plate 2, and the left and right sides of screw mandrel 12 midpoints The two sections on the right are threads with opposite directions of rotation respectively. The lifting motor 16 is arranged at the bottom of the hanging plate 2, and the horizontal output shaft of the lifting motor 16 is connected to one end of the screw mandrel 12; , and the right are horizontally arranged and perpendicular to the screw mandrel 12. The nuts fixed at the center of the two moving plates 13 are threaded with the left and right sections of the screw mandrel 12 respectively, and the pin shafts at both ends of each moving plate 13 are slidably fitted on both sides. In the horizontal groove consistent with the direction of the screw mandrel 12 provided on the plate (built on the hanging plate 2).
所述提升架分别对称设于吊板2宽度方向的两侧,每侧提升架包括两等长的长连杆14和两等长的短连杆15,两长连杆14于中点处铰连呈交叉状,两长连杆14的左、右上端分别与同侧面的左、右移动板13的端头销轴(伸出侧板)铰连,所述两短连杆15分别设于两长连杆14的左、右下端的下方且左、右对称,短连杆15的左、右上端分别与长连杆14的左、右下端铰连,短连杆15的左、右下端分别与清洗板3(位于短连杆15下方)的侧面铰连,在整个运动过程中,短连杆15的左、右上端的间距大于短连杆15左、右下端的间距。Described hoist frame is respectively symmetrically arranged on both sides of the width direction of hanging plate 2, and each side hoist frame comprises two equal-length long connecting rods 14 and two equal-length short connecting rods 15, and two long connecting rods 14 are hinged at the midpoint. Even in a cross shape, the left and right upper ends of the two long connecting rods 14 are respectively hinged with the end pins (extending from the side plates) of the left and right moving plates 13 on the same side, and the two short connecting rods 15 are respectively located on The left and right lower ends of the two long connecting rods 14 are left and right symmetrical, the left and right upper ends of the short connecting rods 15 are respectively hinged with the left and right lower ends of the long connecting rods 14, and the left and right lower ends of the short connecting rods 15 are Respectively hinged with the side of the cleaning plate 3 (located below the short connecting rod 15), in the whole movement process, the distance between the left and right upper ends of the short connecting rod 15 is greater than the distance between the left and right lower ends of the short connecting rod 15.
需要提升清洗板3时,升降电机16启动带动丝杆12正、反向旋转,丝杆12带动移动板13同时相对移动或同时相离移动,移动板13的运动控制两长连杆14而改变其交叉角度,从而实现清洗板3的升降。When the cleaning plate 3 needs to be lifted, the lifting motor 16 starts to drive the screw rod 12 to rotate forward and reverse, and the screw rod 12 drives the moving plate 13 to move relative to each other or move away from each other at the same time, and the motion of the moving plate 13 controls the two long connecting rods 14 to change Its intersection angle, thereby realizes the lifting of cleaning plate 3.
如图4、图5、图6、图7(a)、图7(b),所述清洗装置包括清洗板3以及由喷液头4-1和吸液头4-2集成的清洗头4,所述清洗板3安装于升降机构上,所述清洗头4以12×8的列阵方式设于清洗板3底部。As shown in Figure 4, Figure 5, Figure 6, Figure 7(a), and Figure 7(b), the cleaning device includes a cleaning plate 3 and a cleaning head 4 integrated with a liquid spray head 4-1 and a liquid suction head 4-2 , the cleaning plate 3 is installed on the lifting mechanism, and the cleaning heads 4 are arranged at the bottom of the cleaning plate 3 in an array of 12×8.
所述清洗板3由盖板3-1、吸液板3-2、喷液板3-3组成,所述盖板3-1、吸液板3-2和喷液板3-3均为相同尺寸面积的方板,自上而下,三者贴合固定连接。The cleaning plate 3 is composed of a cover plate 3-1, a liquid absorbing plate 3-2, and a liquid spray plate 3-3, and the cover plate 3-1, the liquid absorbing plate 3-2 and the liquid spray plate 3-3 are all The square boards with the same size and area, from top to bottom, the three are fitted and fixedly connected.
与盖板3-1贴合的吸液板3-2的板面上开设12列与板边平行的吸液槽3-2-1,所述吸液槽3-2-1的两端通过管孔通出吸液板3-2的两侧端面,吸液槽3-2-1的顶面敞口由盖板3-1密封,所述吸液槽3-2-1上均匀间隔开设8个垂直贯通吸液板3-2的吸液孔3-2-2;与吸液板3-2贴合的喷液板3-3的板面上开设12列与吸液槽3-2-1位置相对应的喷液槽3-3-1,所述喷液槽3-3-1的两端通过管孔通出喷液板3-3的两侧端面,喷液槽3-3-1的顶面敞口由吸液板3-2密封,所述喷液槽3-3-1上均匀间隔开设8个与吸液孔3-2-2位置对应且垂直贯通喷液板3-3的喷液孔3-3-2,所述喷液槽3-3-1的宽度大于吸液槽3-2-1的宽度,所述喷液孔3-3-2的直径大于吸液孔3-2-2的直径。There are 12 rows of liquid absorption grooves 3-2-1 parallel to the edge of the plate on the surface of the liquid absorption plate 3-2 attached to the cover plate 3-1, and the two ends of the liquid absorption grooves 3-2-1 pass through The tube holes lead out from both sides of the liquid suction plate 3-2, the top opening of the liquid suction tank 3-2-1 is sealed by the cover plate 3-1, and the liquid suction tank 3-2-1 is evenly spaced 8 liquid suction holes 3-2-2 vertically penetrating the liquid suction plate 3-2; 12 columns and liquid suction grooves 3-2 are provided on the surface of the liquid spray plate 3-3 fitted with the liquid suction plate 3-2 -1 The liquid spray tank 3-3-1 corresponding to the position, the two ends of the liquid spray tank 3-3-1 pass through the tube holes on both sides of the liquid spray plate 3-3, and the liquid spray tank 3-3 The opening of the top surface of -1 is sealed by the liquid-absorbing plate 3-2, and 8 liquid-spraying grooves 3-3-1 are evenly spaced to correspond to the positions of the liquid-absorbing holes 3-2-2 and vertically pass through the liquid-spraying plate 3 -3 spray hole 3-3-2, the width of the spray groove 3-3-1 is greater than the width of the suction groove 3-2-1, and the diameter of the spray hole 3-3-2 is larger than the suction groove The diameter of the liquid hole 3-2-2.
所述喷液头4-1的上部于喷液板3-3的下端面安装于喷液孔3-3-2内,所述吸液头4-2贯通喷液头4-1的中心孔,吸液头4-2的内端(即上端)安装于吸液孔3-2-2内,吸液头4-2的外端(即下端)伸出喷液头4-1的长度为3mm;所述喷液头4-1的上部内圆周均布开设有三个斜向一致的喷射孔4-1-1,所述喷射孔4-1-1的喷射方向与喷液头4-1中心线的夹角均为10°,从而形成旋转喷流;各吸液槽3-2-1两端的管孔分别连通对应设置的吸液机构,各喷液槽3-3-1两端的管孔分别连通对应设置的喷液机构。The upper part of the liquid spray head 4-1 is installed in the liquid spray hole 3-3-2 on the lower end surface of the liquid spray plate 3-3, and the liquid suction head 4-2 passes through the center hole of the liquid spray head 4-1. , the inner end (i.e. the upper end) of the liquid suction head 4-2 is installed in the liquid absorption hole 3-2-2, and the outer end (i.e. the lower end) of the liquid absorption head 4-2 protrudes from the liquid spray head 4-1 to a length of 3mm; the upper inner circumference of the liquid spray head 4-1 is evenly distributed with three spray holes 4-1-1 in the same oblique direction, and the spray direction of the spray hole 4-1-1 is the same as that of the liquid spray head 4-1. The included angles of the center lines are all 10°, thereby forming a rotating jet flow; the pipe holes at both ends of each liquid suction tank 3-2-1 are respectively connected to the corresponding liquid suction mechanism, and the pipe holes at both ends of each liquid spray tank 3-3-1 The holes communicate with correspondingly arranged liquid spraying mechanisms respectively.
所述喷液机构包括喷液管17、喷液瓶18和充气泵,所述喷液管17的进口连通喷液瓶18,喷液管17的出口连通喷液槽3-3-1的两端管孔处,所述喷液瓶18通过气管连通充气泵;所述吸液机构包括吸液管23、吸液瓶24和吸气泵,所述吸液管23的进口连通吸液槽3-2-1的两端管孔处,吸液管23的出口连通吸液瓶24,所述吸液瓶24通过气管连通吸气泵;还设有集液装置,用来收集经过清洗后酶标板21的微孔中残留的经脱水装置脱出的液体,所述集液装置包括集液管19和集液瓶20,所述集液管19连通桶体1底部的排液口和集液瓶20。The liquid spray mechanism comprises a liquid spray pipe 17, a liquid spray bottle 18 and an air pump, the inlet of the liquid spray pipe 17 communicates with the liquid spray bottle 18, and the outlet of the liquid spray pipe 17 communicates with the two sides of the liquid spray tank 3-3-1. At the end pipe hole, the liquid spray bottle 18 is connected to the air pump through the air pipe; the liquid suction mechanism includes a liquid suction pipe 23, a liquid suction bottle 24 and an air suction pump, and the inlet of the liquid suction pipe 23 is connected to the liquid suction tank 3 -At the two ends of the pipe holes of -2-1, the outlet of the suction pipe 23 is connected to the suction bottle 24, and the liquid suction bottle 24 is connected to the suction pump through the trachea; a liquid collection device is also provided to collect the enzyme after cleaning The liquid that remains in the micropores of the target plate 21 through the dehydration device, the liquid collection device includes a liquid collection pipe 19 and a liquid collection bottle 20, and the liquid collection pipe 19 communicates with the liquid outlet and the liquid collection at the bottom of the barrel 1 20 bottles.
上述结构中,各条吸液槽3-2-1和各条喷液槽3-3-1均可独立控制,从而能够实现对酶标板21进行任意单列或是几列、或者整板的清洗工作;各喷液头4-1产生的旋转涡流会使清洗效果更好,清洗效率更高,从整体上大大提高工作效率;吸液头4-2比喷液头4-1长出一部分,能更好的将洗液吸走,使酶标板21微孔中的液体剩余量更少;喷液头4-1和吸液头4-2同时工作,即喷液头4-1向酶标板21喷液的同时,吸液头4-2即刻将液体吸走,喷液不会外溢,有效防止了交叉感染。In the above structure, each liquid suction tank 3-2-1 and each liquid spray tank 3-3-1 can be independently controlled, so that any single column or several columns, or the entire plate can be realized on the microplate plate 21. Cleaning work; the rotating vortex produced by each spray head 4-1 will make the cleaning effect better and the cleaning efficiency higher, greatly improving the work efficiency as a whole; the liquid suction head 4-2 is longer than the liquid spray head 4-1 , can better suck away the washing liquid, so that the remaining amount of liquid in the 21 microwells of the microplate is less; the liquid spray head 4-1 and the liquid suction head 4-2 work at the same time, that is, the liquid spray head 4-1 While the microplate plate 21 is spraying the liquid, the liquid suction head 4-2 immediately sucks the liquid away, so that the sprayed liquid will not overflow, effectively preventing cross-infection.
如图1、图2、图3所示,所述脱水装置设于清洗装置下方,包括护桶7、脱水架8、旋转架9以及脱水电机11带动的传动齿轮10,所述护桶7由上、下两部分构成并竖直安装于桶体1底部,护桶7上、下两部分的端面之间通过滚珠(类似于推力球轴承的结构)同轴安装旋转架9,所述旋转架9的内圈齿轮与设于护桶7内的传动齿轮10啮合,旋转架9的外圈上对称安装竖向的脱水架8,脱水架8的外侧板面上开设12排脱水槽口(对应于酶标板21的各排微孔),脱水架8的内侧板面上部开设酶标板进入口。As shown in Fig. 1, Fig. 2, Fig. 3, described dehydration device is located at the bottom of cleaning device, comprises guard barrel 7, dehydration frame 8, rotating frame 9 and transmission gear 10 driven by dehydration motor 11, and described guard barrel 7 is driven by The upper and lower parts are formed and installed vertically on the bottom of the barrel body 1, and the end faces of the upper and lower parts of the barrel 7 are coaxially installed with a rotating frame 9 through balls (similar to the structure of a thrust ball bearing). The inner ring gear of 9 meshes with the transmission gear 10 located in the protective bucket 7, and the vertical dehydration frame 8 is symmetrically installed on the outer ring of the rotating frame 9, and 12 rows of dehydration notches are provided on the outer plate surface of the dehydration frame 8 (corresponding to In each row of microwells of the microplate plate 21), the upper part of the inner plate surface of the dehydration frame 8 is provided with a microplate plate inlet.
所述结构可以同时对两个酶标板21进行脱水,工作时,由脱水电机11带动传动齿轮10,从而带动旋转架9及脱水架8转动,实现离心脱水,离心脱水的优点是离心力很容易大于酶标板21中液体的吸附力从而使水分脱离,可以根据不同的需要调节转速来达到要求,脱水效率很高,从而在很大程度上提高了工作效率。The structure can dehydrate two microplates 21 at the same time. During work, the dehydration motor 11 drives the transmission gear 10, thereby driving the rotating frame 9 and the dehydration frame 8 to rotate, and realizes centrifugal dehydration. The advantage of centrifugal dehydration is that centrifugal force is easy It is greater than the adsorption force of the liquid in the microplate 21 so that the moisture is separated, and the rotation speed can be adjusted according to different needs to meet the requirements. The dehydration efficiency is very high, thereby improving the work efficiency to a large extent.
如图1、图2、图3所示,所述酶标板支撑移送装置设于清洗装置与脱水装置之间,包括安放酶标板21的支撑架5和翻转推送架6,所述支撑架5安装于护桶7的桶口,支撑架5上设有支撑清洗板3的台阶,此台阶为清洗板3下降的极限,支撑架5上设有导向结构可以使清洗头4准确无误的对准酶标板21的微孔,所述翻转推送架6设于支撑架5底部而处于酶标板21下方,翻转推送架6动作时可将酶标板21从支撑架5一侧推离口送出支撑架5,为配合酶标板21准确进入脱水架8,旋转架9上有定位装置,在工作开始时旋转架9会自动定位,使其中一个脱水架8的内侧板面上部的酶标板进入口对准支撑架5一侧的推离口。As shown in Fig. 1, Fig. 2 and Fig. 3, the support transfer device for the microplate plate is arranged between the cleaning device and the dehydration device, and includes a support frame 5 for placing the microplate plate 21 and an overturning push frame 6, the support frame 5 is installed on the barrel mouth of the protective barrel 7. The support frame 5 is provided with a step supporting the cleaning plate 3. This step is the limit for the cleaning plate 3 to descend. The support frame 5 is provided with a guide structure to make the cleaning head 4 align accurately. The microwell of quasi-enzyme standard plate 21, described flip push frame 6 is arranged at the bottom of support frame 5 and is below the microplate plate 21, when flip push frame 6 moves, enzyme standard plate 21 can be pushed away from support frame 5 side. Send support frame 5, enter dehydration rack 8 accurately for coordinating microplate plate 21, positioning device is arranged on rotary frame 9, and rotary frame 9 can be positioned automatically when work starts, makes the enzyme label on the inner plate surface top of one of dehydration rack 8 The board entry port is aligned with the push-off port on one side of the support frame 5 .
本发明的工作过程为:Working process of the present invention is:
1、从桶体1的桶口人工将酶标板21放到支撑架5上。1. Manually place the microplate plate 21 on the support frame 5 from the mouth of the barrel body 1.
2、脱水架8进行自动定位,使脱水架8的酶标板进入口对准支撑架5一侧酶标板21的推离口。2. The dehydration rack 8 is automatically positioned so that the microplate entry port of the dehydration rack 8 is aligned with the push-out port of the microplate plate 21 on one side of the support frame 5 .
3、提升架将清洗头4下落到指定位置,充气泵开始对装有药液的喷液瓶18充气,同时抽气泵开始对吸液瓶24进行抽气,当达到设定压力时喷液管17上的电磁阀和吸液管23上的电磁阀同时打开,开始对酶标板21进行清洗。3. The lifting frame lowers the cleaning head 4 to the designated position, the air pump starts to inflate the liquid spray bottle 18 containing the liquid medicine, and the air pump starts to pump air to the liquid suction bottle 24 at the same time, when the set pressure is reached, the liquid spray tube The electromagnetic valve on the 17 and the electromagnetic valve on the pipette 23 are opened simultaneously, and the microplate plate 21 is started to be cleaned.
4、清洗完毕后,对喷液瓶18充气的充气泵停止工作,然后喷液管17上的电磁阀先关闭,然后吸液瓶24抽气的吸气泵停止工作,同时吸液管23上的电磁阀关闭。4. After cleaning, the air pump for inflating the liquid spray bottle 18 stops working, then the electromagnetic valve on the liquid spray pipe 17 is closed first, and then the suction pump for sucking the liquid in the liquid suction bottle 24 stops working. The solenoid valve is closed.
5、提升架将清洗头4提起,翻转推送架6向上翻起将已经清洗完毕的酶标板21推送到脱水架8中(如图3所示),推送完成后,旋转架9转动180°使另外一个脱水架8对准支撑架5一侧酶标板21的推离口,与此同时翻转推送架6向下翻回到原位,人工再往支撑架5上放置另一快酶标板21,设备自动重复以上过程。5. Lift the cleaning head 4 by the lifting frame, turn the pushing frame 6 up and push the cleaned microplate plate 21 into the dehydration frame 8 (as shown in Figure 3). After the pushing is completed, the rotating frame 9 rotates 180° Align the other dehydration rack 8 with the push-off opening of the microplate plate 21 on one side of the support frame 5, and at the same time turn the push rack 6 down and return to its original position, and manually place another fast enzyme standard on the support frame 5 Board 21, the device automatically repeats the above process.
6、等翻转推送架6将第二块酶标板21送入第二个脱水架8中后,脱水电机11开始工作,带动旋转架9及脱水架8旋转,从而完成对酶标板21的脱水工作,脱水完成后整个清洗过程结束;脱水过程中,废液集中于桶体1底部,集液管19将废液输出至集液瓶20收集。6. After turning over the pushing rack 6 to send the second microplate plate 21 into the second dehydration rack 8, the dehydration motor 11 starts to work, driving the rotating rack 9 and the dehydration rack 8 to rotate, thereby completing the microplate plate 21 Dehydration work, the whole cleaning process ends after the dehydration is completed; during the dehydration process, the waste liquid is concentrated at the bottom of the barrel body 1, and the liquid collection pipe 19 outputs the waste liquid to the liquid collection bottle 20 for collection.
本发明可同时对两块酶标板21进行清洗和脱水,也可以对一块酶标板21进行清洗和脱水,对一块酶标板21进行处理时要先在另一脱水架8中放入一空的酶标板21作为配重,以避免在在脱水过程中发生震动。The present invention can clean and dehydrate two microplate plates 21 at the same time, and can also clean and dehydrate one microplate plate 21. When one microplate plate 21 is processed, an empty dehydration rack 8 should be put into it first. The microtiter plate 21 is used as a counterweight to avoid vibration during the dehydration process.
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