CN103630700A - Analyzer sample apparatus, and reagent testing method thereof - Google Patents
Analyzer sample apparatus, and reagent testing method thereof Download PDFInfo
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- CN103630700A CN103630700A CN201210300869.9A CN201210300869A CN103630700A CN 103630700 A CN103630700 A CN 103630700A CN 201210300869 A CN201210300869 A CN 201210300869A CN 103630700 A CN103630700 A CN 103630700A
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Abstract
The invention discloses an analyzer sample apparatus. The analyzer sample apparatus comprises a reagent container and a syringe; and is characterized in comprising a sensor, a liquid storage chamber, a first magnetic valve, and a second magnetic valve which are arranged between the reagent container and the syringe, and are connected end to end via a pipeline successively. The head end of the liquid storage chamber and the first magnetic valve are connected via another pipeline. The invention also provides a reagent testing method of the analyzer sample apparatus. According to the reagent testing method, when it is detected by the sensor that no reagent is in the analyzer sample apparatus, a control circuit is used for reminding that no reagent is in the analyzer sample apparatus; and when the amount of the reagent in the liquid storage chamber is enough for one time of sample count, then the control circuit will allow this time of sample count to be performed continuously. The analyzer sample apparatus and the reagent testing method possesses following advantages: cost is relatively low, detection is reliable, the control circuit is sample, and operation is convenient.
Description
Technical field
The present invention relates to cellanalyzer, relate in particular to a kind of analyser sample device and reagent test method thereof.
Background technology
Cellanalyzer need to be used these 3 kinds of reagent of dilution, cleaning fluid and hemolytic agent, during any being finished in these 3 kinds of reagent, instrument can be made warning after the test job that completes current sample, so likely makes last sample before reporting to the police because reagent deficiency causes, measure inaccurate.
The method that the conventional detection reagent of cellanalyzer has or not is at present to use liquid road sensor, liquid road sensor has two kinds of float-type and photo-electrics, while adopting float-type, when reagent is during in low level, instrument detect reagent without signal (float when the lower liquid level instrument prompting reagent without) and this counting does not also finish, this a period of time need reagent, the method that solves the reagent need is during this period of time to regulate float when lower liquid level and the relative height of reagent feed tube road port, reserve than complete machine do once the required amount of reagent of complete counting many 1.2 times above, this method is easy to realize.For float-type, photo-electric detection reagent has or not more reliably attractive in appearance, the detection part of electro-optical pickoff is generally optocoupler, optocoupler is arranged on machine intimate, and reagent is outside machine, by the outer reagent of pipeline and machine, be connected, make to be fixed between the luminotron and receiving tube of optocoupler into the pipeline of reagent, luminotron sends constant light intensity, when having reagent and do not have reagent (to be air or air and bubble, or liquid notes) light intensity that time, receiving tube receives is different, thereby judges having or not of reagent.
Summary of the invention
In order to guarantee that optocoupler detects reagent and can have without male offspring sufficient reagent to complete this test sample book test, the present invention proposes a kind of analyser sample device, it comprises reagent container, syringe, also comprise: from described reagent container to passing through successively the end to end sensor of pipeline, fluid reservoir, the first solenoid valve and the second solenoid valve syringe, wherein, also have a pipeline that the head end of described fluid reservoir is connected with described the first solenoid valve.
Whether preferably, described sensor is optocoupler sensor, for detection of being finished in described reagent container.
Preferably, also comprise the control circuit that is connected in described sensor, for the state to reagent container, point out.
Preferably, described the first solenoid valve and the second solenoid valve are all three-way solenoid valve.
Preferably, described fluid reservoir is a container, and its volume is at least 1.2 times of a required amount of reagent of sample counting.
Preferably, described fluid reservoir is a kind of in spherical, cylindrical, taper.
The present invention also provides a kind of analyser sample device, it comprises reagent container, syringe, it is characterized in that, also comprise: from described reagent container to passing through successively the end to end sensor of pipeline, the 3rd solenoid valve syringe, wherein, the pipeline volume between described sensor and the 3rd solenoid valve is at least more than or equal to the required amount of reagent of sample counting one time.
The present invention also provides a kind of analyser sample device reagent test method, comprises the following steps:
(1) described reagent is when the work of syringe, by flow through successively reagent container, sensor, fluid reservoir, the first solenoid valve, the second solenoid valve of the connection of pipeline, enters syringe inside;
(2) described syringe injects analyser by the second solenoid valve by described reagent;
(3) when sensor detects without reagent, control circuit prompting is without reagent, by the first solenoid valve, the reagent in fluid reservoir is entered to syringe inside via the first solenoid valve and the second solenoid valve, control circuit will allow that this counting proceeds, counted post analysis instrument sample device and quit work and point out without reagent, need to pour into reagent instrument and just can work on.
Preferably, in step (3), when pointing out without reagent, need in reagent container, pour into reagent, during perfusion reagent, syringe is air-breathing, air in fluid reservoir enters in syringe through the first solenoid valve, the second solenoid valve, then the second electromagnetic valve work, syringe pushes away gas, air in syringe is discharged to analyser sample device through another pipeline disposed thereon, and so forth repeatedly, until fluid reservoir, pour into reagent end-of-job while all filling reagent in pipeline and syringe.
Preferably, described the first electromagnetic valve work, described reagent enters into fluid reservoir through reagent container, sensor.
The present invention by arranging fluid reservoir between described sensor and described the first solenoid valve, reagent in fluid reservoir meets the amount of a sample technology, thereby guarantee that optocoupler detects reagent and can have without male offspring sufficient reagent to complete this test sample book test, and only increase a fluid reservoir, cost is simple, easy to operate.
Accompanying drawing explanation
Fig. 1 is analyser sample device liquid of the present invention road schematic diagram;
Fig. 2 is analyser sample device fluid reservoir three-view diagram of the present invention;
Fig. 3 is another embodiment schematic diagram of analyser sample device of the present invention.
Embodiment
With reference to the accompanying drawings, describe analyser sample device of the present invention and test sample method thereof in detail.As shown in Figure 1, be analyser sample device liquid of the present invention road schematic diagram, it comprises reagent container 1, sensor 2, fluid reservoir 3, the first solenoid valve 4, the second solenoid valve 5 and syringe 6 analyser sample device of the present invention.
From reagent container 1 to passing through successively the end to end sensor 2 of pipeline 7,13,9,10,11, fluid reservoir 3, the first solenoid valve 4 and the second solenoid valve 5 syringe 6, wherein, also have a pipeline 8 that the head end of fluid reservoir 3 is connected with the first solenoid valve 4.
Wherein, the first solenoid valve and the second solenoid valve are three-way solenoid valve.Three ends of the first solenoid valve are connected to the head end of the second solenoid valve, fluid reservoir and the tail end of fluid reservoir.
Three ends of the second solenoid valve are connected to syringe, the first solenoid valve and by the connected analyser of pipeline (not indicating).
Whether sensor 2 of the present invention is optocoupler sensor, for detection of being finished in reagent container 1.In the present embodiment, a control circuit (not indicating) that is connected in described sensor can also be set, for the state to reagent container, point out.
Described fluid reservoir 3 is containers, in the present embodiment, its volume is at least 1.2 times of a required amount of reagent of sample counting, and to guarantee the requirement of a sample counting, and described fluid reservoir 3 is for being spherical, cylindrical, taper or other any shapes that is applicable to doing container.As shown in Figure 2, in one embodiment, fluid reservoir 3 polycrystalline substances are high in the middle of being, edge is low, and the joint that has an adapter in bottom center, fluid reservoir 3 lid structures and fluid reservoir 3 polycrystalline substances are similar, just at edge to the part between centre many a joint; The connection of fluid reservoir 3 lids and fluid reservoir 3 bodies is to stick with glue to connect, and guarantees nothing leakage under long duration of action under its 3 kilograms of pressure.
The present invention also provides a kind of analyser sample device test sample method, it comprises above-mentioned analyser sample device, control circuit and the reagent for testing, described reagent is positioned in described reagent container 1 and described fluid reservoir 3, and described test sample method comprises the following steps:
(1) described reagent enters syringe 6 inside by flow through successively sensor 2, fluid reservoir 3, the first solenoid valve 4, the second solenoid valve 5 of the connection of pipeline under the work of syringe;
(2) syringe 6 injects analyser (do not indicate) by reagent by the second solenoid valve 5 by another pipeline disposed thereon;
(3) when sensor 2 detects without reagent, control circuit prompting is without reagent, reagent in fluid reservoir 3 meets the amount of a sample technology, control circuit will allow that this counting proceeds, counted post analysis instrument sample device and quit work and point out without reagent, need to pour into reagent container 1 and just can work on.When pointing out without reagent, need in reagent container 1, pour into reagent, during perfusion reagent, syringe 6 is air-breathing, the first solenoid valve 4 work, reagent is through reagent container 1, sensor 2 enters into fluid reservoir 3, siphunculus road 7 in air process in fluid reservoir 3, the first solenoid valve 4, the second solenoid valve 5 enters in syringe 6, then the second solenoid valve 5 work, syringe 6 pushes away gas, air in syringe 6 is discharged to analyser sample device through another pipeline disposed thereon, and so forth repeatedly, until fluid reservoir 3, whole pipelines and syringe 6 is interior pours into reagent end-of-job while all filling reagent, described analyser sample device restarts work.The trigger condition that reagent is walked solenoid valve 4 by pipeline 9 and pipeline 10 be when normal counting valve 4 in normally off; The trigger condition that reagent is walked solenoid valve 4 by pipeline 8 and pipeline 10 be when perfusion dilution valve 4 in normally open; The trigger condition that reagent is walked solenoid valve 5 by pipeline 10 and pipeline 11 is that valve 5 is in normally off; The trigger condition that reagent is walked solenoid valve 5 by pipeline 11 and pipeline 12 is that valve 5 is in normally open.
The present invention by arranging fluid reservoir 3 between described sensor 2 and described the first solenoid valve 4, reagent in fluid reservoir 3 meets the amount of a sample technology, thereby guarantee that optocoupler detects reagent and can have without male offspring sufficient reagent to complete this test sample book test, and only increase a fluid reservoir 3, cost is simple, easy to operate.
As described in Figure 3, be another embodiment schematic diagram of analyser sample device of the present invention, in the present embodiment, from described reagent container to passing through successively the end to end sensor of pipeline, the 3rd solenoid valve syringe.That is, with one section of pipeline, replace fluid reservoir 3 and the first solenoid valve 4 in Fig. 1, the capacity from the pipeline between sensor and the 3rd solenoid valve require increasing is greater than the amount that has equaled the required reagent of sample.
This scheme advantage is that the parts that use are few, and shortcoming is that pipeline is oversize, higher to syringe performance requirement, not attractive in appearance.In the less place of required amount of reagent can adopt the present invention, optimal case substitutes.
The foregoing is only the preferred embodiments of the present invention; not thereby limit the scope of the claims of the present invention; every equivalent structure or flow process conversion that utilizes instructions of the present invention and accompanying drawing content to do; or be directly or indirectly used in other relevant technical field, be all in like manner included in scope of patent protection of the present invention.
Claims (10)
1. an analyser sample device, it comprises reagent container, syringe, it is characterized in that, also comprise: from described reagent container to passing through successively the end to end sensor of pipeline, fluid reservoir, the first solenoid valve and the second solenoid valve syringe, wherein, also have a pipeline that the head end of described fluid reservoir is connected with described the first solenoid valve.
2. whether analyser sample device according to claim 1, is characterized in that: described sensor is optocoupler sensor, for detection of being finished in described reagent container.
3. analyser sample device according to claim 2, is characterized in that: also comprise the control circuit that is connected in described sensor, for the state to described reagent container, point out.
4. analyser sample device according to claim 1, is characterized in that: described the first solenoid valve and described the second solenoid valve are all three-way solenoid valve.
5. analyser sample device according to claim 1, is characterized in that: described fluid reservoir is a container, and its volume is at least 1.2 times of a required amount of reagent of sample counting.
6. analyser sample device according to claim 1, is characterized in that: described fluid reservoir is a kind of in spherical, cylindrical, taper.
7. an analyser sample device, it comprises reagent container, syringe, it is characterized in that, also comprise: from described reagent container to passing through successively the end to end sensor of pipeline, the 3rd solenoid valve syringe, wherein, the pipeline volume between described sensor and the 3rd solenoid valve is at least more than or equal to the required amount of reagent of sample counting one time.
8. an analyser sample device reagent test method, applies analyser sample device as claimed in claim 3, it is characterized in that: described reagent test method comprises the following steps:
(1) described reagent is when syringe is worked, and by flow through successively reagent container, sensor, fluid reservoir, the first solenoid valve, the second solenoid valve of the connection of pipeline, enters syringe inside;
(2) described syringe injects analyser by the second solenoid valve by described reagent;
(3) when sensor detects without reagent, control circuit prompting is without reagent, by the first solenoid valve, the reagent in fluid reservoir is entered to syringe inside via the first solenoid valve and the second solenoid valve, control circuit will allow that this counting proceeds, counted post analysis instrument sample device and quit work and point out without reagent, need to pour into reagent instrument and just can work on.
9. analyser sample device reagent test method according to claim 8, it is characterized in that: in step (3), when pointing out without reagent, need in reagent container, pour into reagent, during perfusion reagent, syringe is air-breathing, air in fluid reservoir is through the first solenoid valve, the second solenoid valve enters in syringe, then the second electromagnetic valve work, syringe pushes away gas, air in syringe is discharged to analyser sample device through another pipeline disposed thereon, and so forth repeatedly, until fluid reservoir, while all filling reagent in pipeline and syringe, pour into reagent end-of-job.
10. analyser sample device reagent test method according to claim 9, is characterized in that: described the first electromagnetic valve work, described reagent enters into fluid reservoir through reagent container, sensor.
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| Application Number | Priority Date | Filing Date | Title |
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| CN201210300869.9A CN103630700A (en) | 2012-08-22 | 2012-08-22 | Analyzer sample apparatus, and reagent testing method thereof |
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| CN201210300869.9A CN103630700A (en) | 2012-08-22 | 2012-08-22 | Analyzer sample apparatus, and reagent testing method thereof |
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Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019000459A1 (en) * | 2017-06-30 | 2019-01-03 | 深圳迈瑞生物医疗电子股份有限公司 | Liquid-supply device, sample-analyzing apparatus and liquid-supply method |
| CN110346588A (en) * | 2018-04-04 | 2019-10-18 | 深圳市帝迈生物技术有限公司 | A kind of reagent consumption modification method, reagent liquid injection system and blood analyser |
| CN110672869A (en) * | 2019-09-18 | 2020-01-10 | 东软威特曼生物科技（沈阳）有限公司 | Device and method for detecting needle blockage of sample needle |
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| KR101322543B1 (en) * | 2006-03-10 | 2013-10-28 | 비비아 바이오테크 에스.엘. | High Speed Sample Supply Device |
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| KR101322543B1 (en) * | 2006-03-10 | 2013-10-28 | 비비아 바이오테크 에스.엘. | High Speed Sample Supply Device |
| US20080250876A1 (en) * | 2007-04-16 | 2008-10-16 | Bingqiang Zhao | Flow cytometric apparatus and method |
| CN201974429U (en) * | 2010-12-31 | 2011-09-14 | 上海奥普生物医药有限公司 | Automatic liquid adding device used in medical rapid detection colloidal gold immunochromatographic assay dry-type digital reader |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2019000459A1 (en) * | 2017-06-30 | 2019-01-03 | 深圳迈瑞生物医疗电子股份有限公司 | Liquid-supply device, sample-analyzing apparatus and liquid-supply method |
| CN110346588A (en) * | 2018-04-04 | 2019-10-18 | 深圳市帝迈生物技术有限公司 | A kind of reagent consumption modification method, reagent liquid injection system and blood analyser |
| CN110672869A (en) * | 2019-09-18 | 2020-01-10 | 东软威特曼生物科技（沈阳）有限公司 | Device and method for detecting needle blockage of sample needle |
| CN110672869B (en) * | 2019-09-18 | 2023-05-02 | 东软威特曼生物科技（沈阳）有限公司 | Device and method for detecting blocking of sample needle |
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Inventor after: You Fan Inventor after: Zhou Shumin Inventor after: Huang Shi Inventor after: Fan Jianping Inventor before: You Fan Inventor before: Wang Qingsong Inventor before: Wu Wenbin |
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Free format text: CORRECT: INVENTOR; FROM: YOU WANG QINGSONG WU WENBIN TO: YOU ZHOU SHUMIN HUANG SHI FAN JIANPING |
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Application publication date: 20140312 |