CN103622673A - Autofluorescent fault molecular imaging equipment compatible with magnetic resonance - Google Patents
Autofluorescent fault molecular imaging equipment compatible with magnetic resonance Download PDFInfo
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- CN103622673A CN103622673A CN201310562303.8A CN201310562303A CN103622673A CN 103622673 A CN103622673 A CN 103622673A CN 201310562303 A CN201310562303 A CN 201310562303A CN 103622673 A CN103622673 A CN 103622673A
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Abstract
The invention discloses autofluorescent fault molecular imaging equipment compatible with magnetic resonance. The autofluorescent fault molecular imaging equipment comprises optical imaging equipment and a guide rail; the optical imaging equipment comprises a shielding case and an optical camera obscura, wherein an imaging device is arranged inside the optical camera obscura; the two sides of the guide rail are located in existing medical magnetic resonance equipment and the optical imaging equipment respectively, the guide rail is provided with a carrying platform used for carrying a small animal, and the carrying platform can slide on the guide rail; the shielding case isolates mutual interference between the magnetic resonance equipment and the optical imaging equipment; the carrying platform guarantees that the small animal does not need to be hung and the posture of the small animal does not change in the magnetic resonance imaging process and the optical camera process. A two-sided reflector and rotating wheels are adopted so that the optical imaging equipment can acquire multi-angle multi-spectral autofluorescent data in one plane. High-precision three-dimensional imaging is realized based on the autofluorescent data in combination with structural data provided by the magnetic resonance equipment.
Description
Technical field
The present invention relates to a kind of autofluorescence tomography molecule image equipment of magnetic resonance compatible, in particular, relate to multispectral in conjunction with magnetic resonance, multi-angle autofluorescence Laminographic device can be realized the noinvasive Real-time and Dynamic imaging in vivo of organism internal physiological or pathological process on cell, gene and molecular level.
Background technology
According to Weissleder, proposed the concept of molecular image, all biomedical imaging methods that can at body, bioprocess be characterized and be measured at cell and molecular level all belong to the category of molecular image.Common molecular image mode mainly comprises radio nuclide imaging at present, as positron emission tomography, single photon emission computerized tomography, nuclear magnetic resonance, and optical imagery, ultra sonic imaging etc.The imaging technique of each mode has pluses and minuses separately, and difference between each mode is mainly reflected in time or spatial resolution, penetration depth, imaging cost, sensitivity and the aspects such as harm to imaging object.Wherein, compare with other imaging in vivo technology, that optical molecular image has advantages of is easy and simple to handle, measure fast, visual result, radiationless, high sensitivity and many uniquenesses such as equipment cost is lower, developed into a kind of desirable living small animal formation method, and in fields such as life sciences, medical research and medicament research and development, there is widely an application, but, at present still in initial stage, at aspects such as imaging algorithm, imaging systems, all exist crucial problem in science and not yet solve in the research of bulk optics molecular imaging technology.
Be published on Nature magazine in February, 2010 be entitled as " Animal Imaging-the Whole Picture " iconography commentary to application scenario and the parameter of several main imaging techniques in research and application stage compare at present.The article pointed out in view of various image modes benefit and limitation separately, multiple imaging mode is merged (as PET/CT, SPECT/CT, PET/MR, MR/CT etc.), be so-called multi-modality imaging (Multimodalities), the image that builds multidimensional, colour is best selection.In biomedical science field, the content that adopts multi-modal mode to carry out correlational study gets more and more in recent years.
Chinese invention patent description CN101301192B discloses a kind of multimode autofluorescence tomography imaging device in the prior art, in this device signal acquisition module, adopt toy to hang rotation multi-angle and gather fluorescence and X-ray signal, carry out multi-modal fusion, rebuild organism internal light source position.X-ray is not fine to soft-tissue imaging effect, and the deformation of toy suspension attitude internal organs is larger, and single spectrum data are insufficient, and it is unfavorable all reconstruction effect to be brought.A kind of multispectral fluorescence fault imaging device is also disclosed in Chinese utility model patent description CN202568208U, this device adopts optical filter to obtain multiple fluoroscopic images and is used for optical 3-dimensional and rebuilds, but what adopt is positive single angle optical signalling, and do not provide other mode imagings of employing to provide organizational structure for three-dimensional reconstruction.
Summary of the invention
For the defect existing in prior art, the object of the present invention is to provide a kind of autofluorescence tomography molecule image equipment of magnetic resonance compatible, comprising:
Optical image equipment, described optical image equipment is provided with shielding case and optics camera bellows, in described optics camera bellows, is provided with device for image;
One guide rail, described guide rail one end is arranged in described optical image equipment, and described guide rail is provided with the plummer of carrying toy, and described plummer can slide on guide rail.
On the basis of technique scheme, in described object stage, be provided with heating cushion.
On the basis of technique scheme, described object stage is provided with two-face mirror.
On the basis of technique scheme, described object stage top is provided with camera lens, and described camera lens is fixed on described Optical Dark Solitary Pulse box top.
On the basis of technique scheme, between described object stage and described camera lens, be provided with swiveling wheel, described swiveling wheel is provided with the bandpass filter of different centre wavelengths.
On the basis of technique scheme, described optics camera bellows is located in described shielding case, and described shielding case is provided with external shield door and inner shield door, between described external shield door and inner shield door, forms spatial transition.
On the basis of technique scheme, described optics camera bellows is light-proof material, and described shielding case is high-permeability material.
On the basis of technique scheme, described guide rail is provided with toy coil.
The autofluorescence tomography molecule image equipment of magnetic resonance compatible provided by the invention, with magnetic resonance equipment cooperating, described guide rail one end is arranged in described optical image equipment, the other end is arranged in described magnetic resonance equipment, and described plummer can slide along described guide rail between described optical image equipment and described magnetic resonance equipment.
Beneficial effect of the present invention is: adopts magnetic shield design, optical image equipment is placed in to Magnetic Resonance (MR) Room/ MRI Room, by guide rail, transmit, keep toy attitude constant, and very important for optical 3-dimensional cross sectional reconstruction.Double-deck isolating door design, the completely isolated magnetic field of magnetic resonance equipment and the phase mutual interference of described optical image equipment.
Adopt magnetic resonance that toy structural information is provided, compare the method for traditional employing computer tomography, there is higher soft tissue spatial resolution, be conducive to cut apart more accurately toy internal soft tissues, thereby improve optical 3-dimensional cross sectional reconstruction precision.
Adopt two-face mirror and swiveling wheel, make optical image equipment in a plane, can photograph multi-angle, multispectral autofluorescence information.More data, have reduced the theoretic pathosis of autofluorescence fault imaging.
Conventional junction is closed the autofluorescence system of computer tomography, and toy is placed in suspension status, and internal's distortion is large and difficult fixing, and experiment is inconvenient.The present invention adopts plummer, and toy, without suspension, is tested more convenient.
Accompanying drawing explanation
Fig. 1 is the autofluorescence tomography molecule image equipment structure chart of a kind of magnetic resonance compatible of the present invention;
Fig. 2 is shielding case structural representation of the present invention;
Fig. 3 is optical image device structure schematic diagram of the present invention;
The flow chart of Fig. 4 for using the autofluorescence tomography molecule image equipment of a kind of magnetic resonance compatible of the present invention to test.
The specific embodiment
Below in conjunction with drawings and Examples, the present invention is described in further detail.
Please refer to Fig. 1, Fig. 1 is a kind of autofluorescence tomography molecule image equipment structure chart of magnetic resonance compatible, optics camera bellows 105 is realized and being docked with magnetic resonance equipment 101 by guide rail 104, guarantees that toy 102 attitudes are unified, guarantees that the internal organs of toy in subsequent step deformation can not occur.Optical signalling and magnetic resonance scan successively.Toy 102 is sent in optics camera bellows 105 and toy coil 103 by guide rail 104.
Please refer to Fig. 2, Fig. 2 is shielding case structural representation; How demonstration toy 102 enters cabin 205 in optics camera bellows.Toy 102 is placed on guide rail 104.Outer hatch door 202, interior hatch door 204 is all in closed condition.First outer hatch door 202 is opened, and toy 102 moves to transfer chamber 203 places automatically along guide rail 104.Outer hatch door 202 is closed, and interior hatch door 204 is opened.Toy 102 moves to 205 places, interior cabin along guide rail 104, and interior hatch door 204 is closed.Toy 102 delivers from vault adopt contrary flow process.Shielding case material is permalloy, reaches the effect of optical device and the isolation of magnetic resonance equipment magnetic field.
Fig. 3 is optical image equipment schematic diagram.It comprises shielding case 301 and optics camera bellows 302, and aerofoil fan 303,315 forms convection current wind, for camera, dispels the heat.High sensitivity EMCCD camera 304 and camera lens 305 are for taking optical signalling.Camera lens 305 is fixed on swiveling wheel 306 tops, and optical filter 307 is placed in the place, different hole of swiveling wheel 306.Can gather multispectral optical signalling.Swiveling wheel 306 can photograph the optical signalling of different wave length by rotation, thereby obtains multispectral information.LED white light 308,314 can provide camera bellows 302 interior white light illumination, facilitates the white light surface of toy 102 to take.High reflectivity film reflecting mirror 309,313 provides the optical information of two sides, realizes multi-angle simultaneously and gathers optical signalling.Object stage 311 adopts upper and lower removable design to make optical focusing more flexible.The inner heating cushion 312 that adopts of object stage 311 can guarantee in experimentation, toy thermostasis.
Fig. 4 is that toy is tested complete experiment flow figure.In conjunction with this equipment workflow of real animals experiment detailed annotation.Toy 102 pretreatment are anaesthetized accordingly, and injection substrate, is fixed on guide rail 104.Fan 303,315 is opened, and camera 304 switches on power, and adopts quasiconductor to be cooled to operating temperature, and heating cushion 312 is opened, and adjustable lens 305 focal lengths and object stage 311 height, carry out optical focusing.Toy 102 is sent into toy coil 103, and scan with the scanning chamber that toy coil 103 enters magnetic resonance equipment 101 together.Next, on the one hand, adopt semi-automatic method to cut apart the magnetic resonance volume data of obtaining, obtain toy body surface profile and organ contours, and carry out subdivision.In optical dark room 302, carry out on the other hand optical pickup.Toy 102 is transported to optical device doorway by guide rail 104, according to entering cabin step shown in Fig. 2, enters optics camera bellows inner.By controlling swiveling wheel 306 rotations, switch different wave length filter plate 307 and carry out fluorescence signal shooting, time of exposure is looked fluorescent intensity adjustment, between 10s～180s.Take fluorescence signal, need to carry out white light shooting.Swiveling wheel 306 switches to free of light filter place, and LED white light 308,314 is opened, and takes white light signal, time of exposure 0.1s.Optical signalling is taken and is finished, carry out registration and the surface mapping of optical signalling and MR data, adopt the mode based on toy 102 profiles automatically to carry out MR data registration, and the free-space light propagation model based on Lambertian source carry out surperficial light intensity mapping.Employing, based on diffusion model, adopts the method for finite element, carries out accurate light source position and size reconstruction.
The present invention adopts magnetic shield design, and optical device is placed in to Magnetic Resonance (MR) Room/ MRI Room, by guide rail, transmits, and keeps toy attitude constant, very important for optical 3-dimensional cross sectional reconstruction.Double-deck isolating door design, the phase mutual interference of completely isolated magnetic field and equipment.
Adopt magnetic resonance that organism structure information is provided, than the method for traditional employing computer tomography, there is better soft tissue resolution characteristic, and in optics is rebuild, the accurate imaging of soft tissue has a significant impact tissue segmentation.
Adopt swiveling wheel to control multispectral collection, and the design of many minute surfaces, make a plane can photograph multi-angle, multispectral autofluorescence information.More data, have reduced the theoretic pathosis of autofluorescence fault imaging.
Than the autofluorescence system of traditional combination computer tomography, toy is placed in suspension status, internal is out of shape larger, and toy is difficult fixing in suspension status, often makes troubles.The present invention adopts minute surface to take optical signalling and by magnetic resonance acquisition structural information, toy, without suspension, is tested more convenient.
The present invention is not limited to above-mentioned embodiment, for those skilled in the art, under the premise without departing from the principles of the invention, can also make some improvements and modifications, within these improvements and modifications are also considered as protection scope of the present invention.The content not being described in detail in this description belongs to the known prior art of professional and technical personnel in the field.
Claims (9)
1. an autofluorescence tomography molecule image equipment for magnetic resonance compatible, is characterized in that, comprising:
Optical image equipment, described optical image equipment is provided with shielding case and optics camera bellows, in described optics camera bellows, is provided with device for image;
One guide rail, described guide rail one end is arranged in described optical image equipment, and described guide rail is provided with the plummer of carrying toy, and described plummer can slide on guide rail.
2. the autofluorescence tomography molecule image equipment of a kind of magnetic resonance compatible as claimed in claim 1, is characterized in that: in described object stage, be provided with heating cushion.
3. the autofluorescence tomography molecule image equipment of a kind of magnetic resonance compatible as claimed in claim 1, is characterized in that: described object stage is provided with two-face mirror.
4. the autofluorescence tomography molecule image equipment of a kind of magnetic resonance compatible as claimed in claim 1, is characterized in that: described object stage top is provided with camera lens, and described camera lens is fixed on described Optical Dark Solitary Pulse box top.
5. the autofluorescence tomography molecule image equipment of a kind of magnetic resonance compatible as claimed in claim 4, is characterized in that: between described object stage and described camera lens, be provided with swiveling wheel, described swiveling wheel is provided with the bandpass filter of different centre wavelengths.
6. the autofluorescence tomography molecule image equipment of a kind of magnetic resonance compatible as claimed in claim 1, it is characterized in that: described optics camera bellows is located in described shielding case, and described shielding case is provided with external shield door and inner shield door, between described external shield door and inner shield door, form spatial transition.
7. the autofluorescence tomography molecule image equipment of a kind of magnetic resonance compatible as claimed in claim 1, is characterized in that: described optics camera bellows is light-proof material, and described shielding case is high-permeability material.
8. the autofluorescence tomography molecule image equipment of a kind of magnetic resonance compatible as claimed in claim 1, is characterized in that: on described guide rail, be also provided with toy coil.
9. the autofluorescence tomography molecule image equipment of a magnetic resonance compatible, with magnetic resonance equipment cooperating, it is characterized in that: the autofluorescence tomography molecule image equipment of described magnetic resonance compatible is provided with the autofluorescence tomography molecule image equipment of the magnetic resonance compatible as described in claim 1 to 8 any one, and described guide rail one end is arranged in described optical image equipment, the other end is arranged in described magnetic resonance equipment, and described plummer can slide along described guide rail between described optical image equipment and described magnetic resonance equipment.
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103989524A (en) * | 2014-05-22 | 2014-08-20 | 西安电子科技大学 | Small animal imaging bearing system and small animal imaging system |
| CN107080538A (en) * | 2017-03-21 | 2017-08-22 | 中国医学科学院北京协和医院 | Toy autofluorescence and magnetic resonance bimodal molecule fusion of imaging system and method |
| CN107184181A (en) * | 2017-05-15 | 2017-09-22 | 清华大学 | The processing method and system of Dynamic Fluorescence molecular tomographic |
| CN108236474A (en) * | 2016-12-27 | 2018-07-03 | 台达电子工业股份有限公司 | Radiography scanning device, bed and bed positioning device |
| CN114019760A (en) * | 2021-11-16 | 2022-02-08 | 中国科学院长春光学精密机械与物理研究所 | A Small Animal Living Body Bioluminescence Multi-band Stereo Imaging System |
| CN115019589A (en) * | 2022-07-19 | 2022-09-06 | 天津医科大学 | Optical-based intelligent CT teaching simulation system |
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| CN1735802A (en) * | 2002-11-11 | 2006-02-15 | 阿特艾得凡科技研究公司 | Time discrimination optics imaging method and equipment used for part biological tissues of animal body |
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Cited By (8)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103989524A (en) * | 2014-05-22 | 2014-08-20 | 西安电子科技大学 | Small animal imaging bearing system and small animal imaging system |
| CN108236474A (en) * | 2016-12-27 | 2018-07-03 | 台达电子工业股份有限公司 | Radiography scanning device, bed and bed positioning device |
| CN108236474B (en) * | 2016-12-27 | 2021-04-27 | 台达电子工业股份有限公司 | Imaging scanning device, bed carrier and bed carrier positioning device |
| CN107080538A (en) * | 2017-03-21 | 2017-08-22 | 中国医学科学院北京协和医院 | Toy autofluorescence and magnetic resonance bimodal molecule fusion of imaging system and method |
| CN107184181A (en) * | 2017-05-15 | 2017-09-22 | 清华大学 | The processing method and system of Dynamic Fluorescence molecular tomographic |
| CN114019760A (en) * | 2021-11-16 | 2022-02-08 | 中国科学院长春光学精密机械与物理研究所 | A Small Animal Living Body Bioluminescence Multi-band Stereo Imaging System |
| CN115019589A (en) * | 2022-07-19 | 2022-09-06 | 天津医科大学 | Optical-based intelligent CT teaching simulation system |
| CN115019589B (en) * | 2022-07-19 | 2023-11-28 | 天津医科大学 | Intelligent CT teaching simulation system based on optics |
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