CN103616526A - Full-automatic immuoblotting analysis meter and usage method and application thereof - Google Patents
Full-automatic immuoblotting analysis meter and usage method and application thereof Download PDFInfo
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- CN103616526A CN103616526A CN201310689074.6A CN201310689074A CN103616526A CN 103616526 A CN103616526 A CN 103616526A CN 201310689074 A CN201310689074 A CN 201310689074A CN 103616526 A CN103616526 A CN 103616526A
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- 230000001360 synchronised effect Effects 0.000 claims abstract description 21
- 238000001035 drying Methods 0.000 claims abstract description 14
- 230000002572 peristaltic effect Effects 0.000 claims abstract description 14
- 238000003119 immunoblot Methods 0.000 claims description 21
- 238000003556 assay Methods 0.000 claims description 19
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- 238000002038 chemiluminescence detection Methods 0.000 description 1
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Abstract
The application of the invention provides a full-automatic immuoblotting analysis meter. The full-automatic immuoblotting analysis meter comprises an enclosure, wherein a control device, a support device, a peristaltic pump device, a shaking table device, a drying and absorption liquid adding device and a reading device are arranged in the enclosure, the control device comprises a power supply and a control circuit board, the support device is arranged in the enclosure and comprises a shell, a stepping motor and a synchronous belt driven by the stepping motor, the stepping motor and the synchronous belt are arranged in the shell, the synchronous belt drives a first fixed block and a second fixed block, the first fixed block drives the drying and absorption liquid adding device, the second fixed block drives the reading device, the peristaltic pump device is arranged on the support device, the shaking table device is arranged below the front of the support device, the drying and absorption liquid adding device is arranged above a tray rack and is connected with a synchronous belt of the support device through the first fixed block, and the reading device is arranged above the tray rack, is connected with the synchronous belt of the support device through the second fixed block, and comprises a camera, a light source and an input module for inputting acquired data into a computer for processing.
Description
Technical field
The present patent application relates to a kind of full-automatic immunoblotting assay instrument and using method and application, belongs to medical treatment detection device technical field.
Background technology
Western blotting (immunoblotting) claims again Western blotting (Western blotting), is according to the method for certain albumen in the specific binding detection of complex sample of antigen-antibody.This method is a kind of new immune biochemical technology growing up in gel electrophoresis and solid-phase immunoassay technical foundation.Because Western blotting has the high resolution of SDS-PAGE and the high specific of solid-phase immunoassay and susceptibility, now become a kind of routine techniques of analysis of protein.Western blotting is usually used in identifying certain albumen, and can carry out quantitative and semi-quantitative analysis to albumen, in conjunction with chemiluminescence detection, and the expression difference of more a plurality of samples albumen of the same race simultaneously.
Western blot detects by antibody and the epitope generation specific immune response that the target protein that is attached to solid support presents, and whole process comprises: the several parts of the separation of protein, electrotransfer, immunology detection and input.At present, detection for Western blot has manual detection and instrument to detect two large classes, the method manually detecting need to be changed different reagent, operation has increased experimenter's working strength and has lost time frequently, and manual operations is loaded down with trivial details and complicated, people contacts many with tool latent infection article, detect poor stability; Current Western blotting detecting instrument used clinically, although be called " automatically ", in fact can not complete detecting the drying function of sample, and after reaction finishes, detection sample need to be shifted out to instrument and carry out other determination and analysis, waste time and energy and operate inconvenience.
Summary of the invention
The present patent application is the existing above-mentioned weak point of immunoblotting assay instrument of using in analyzing for current detection, manufacture and design a kind of by hatching, the various functions such as washing, liquid feeding, oven dry, result scanning and output preservation is incorporated into the full-automatic immunoblotting assay instrument of one.
Specifically, the full-automatic immunoblotting assay instrument described in the present patent application, comprises casing, is provided with control device, bracing or strutting arrangement, peristaltic-type pump arrangement, shaking table device, dry and add liquid sucking device and interpretation device in casing,
-control device comprises power supply and control circuit board;
-bracing or strutting arrangement is arranged on casing inside, comprise shell and stepper motor in the enclosure and the synchronous belt being driven by stepper motor are set, synchronous belt drives the first fixed block and the second fixed block, and the first fixed block drives dry and adds liquid sucking device, and the second fixed block drives interpretation device;
-peristaltic-type pump arrangement is positioned on bracing or strutting arrangement, comprise support and be arranged on the reagent bottle bracket with a plurality of reagent bottle fixed orifices on support, support rear is provided with pump support, and a plurality of peristaltic pumps are installed on pump support, and each peristaltic pump stretches in reagent bottle by flexible pipe respectively;
-shaking table device arranges bracing or strutting arrangement front lower place, comprises swing motor and the tray rack being driven by swing motor, and tray rack is provided with a plurality of board slots that detect mould bar that hold, and tray rack is driven and waved by transmission shaft by swing motor;
-be dried and add the top that liquid sucking device is positioned at tray rack, and be connected with the synchronous belt of bracing or strutting arrangement by the first fixed block, comprise the drying device that one is installed and add liquid sucking device, drying device comprises motor and by the fan of driven by motor, fan below is provided with heating plate, adds liquid sucking device and comprises the guide rail with slide block, and guide rail is fixed in guide rail fixed plate, guide rail pipe fixed head is fixed on the main fixed head of module, and guide rail is provided with for fixedly adding the main fixed head of pipe of pipette;
-interpretation device is positioned at the top of tray rack, and is connected with the synchronous belt of bracing or strutting arrangement by the second fixed block, comprises camera, light source and the load module that the data input computing machine collecting is processed.
Further, described casing comprises base plate, postnotum, two side plates and front shroud, and front shroud and two side plates are hinged, can realize opening and closing, and the front end of front shroud is also provided with the balustrade panel of convenient operation.
Further, the shell of described bracing or strutting arrangement comprises front apron, backboard, two side back up pads of both sides is set, and the upper backup pad and the lower supporting plate that are arranged on upper and lower both sides, in shell, be also provided with slide rail, the first fixed block and the second fixed block slide along slide rail under the drive of synchronous belt, front apron is provided with strip opening, and the first fixed block and the second fixed block expose to described strip opening.
Further, described bracing or strutting arrangement also comprises the drain bar being arranged on shell, for receiving the liquid being spilt by peristaltic pump, is also provided with in the enclosure for the fixing drag chain of wire rod.
Further, described peristaltic-type pump arrangement also comprises the fan for dispelling the heat being arranged in support.
Further, described shaking table device also comprise left socle, right support and be arranged on left socle and right support between connecting rod, tray rack is fixed on connecting rod by several link supports framves, thereby swing motor drives left socle or right support to drive tray rack to wave, on the support of left and right, be respectively equipped with left support frame and right support frame, on tray rack one side edge, be provided with side cover plate.
Further, described shaking table device also comprise be arranged on tray rack one side for storing the waste liquid pool that detects waste liquid, waste liquid pool is provided with waste liquid pool cover plate.
Further, described interpretation device comprises interpretation crust of the device, and interpretation crust of the device comprises two side covers of top cover, upper cover, lower cover, protecgulum and both sides, and camera is fixed on top cover inwall by lens supporting structure, described light source is LED chip, and LED chip is arranged on light source fixed head.
Further, described dry with add that liquid sucking device also comprises left cover, right cover plate, front shroud and for fixing described dry bracing frame and module main supporting plate with adding liquid sucking device, module main supporting plate is provided with pipe banking stop.
Full-automatic immunoblotting assay instrument described in the present patent application, it is a clinical examination utility appliance detecting for Western blotting, can automatically once carry out the whole process from sample incubation to quantitative test, can support the use with supporting HP immunoblotting kit, once move maximum 48 film bars, in testing process, by 10 peristaltic pumps, carry out reagent distribution, then adopting shaking table to swing up and down the mode of hatching makes to detect film bar and fully contacts with reagent, complete and hatch and washing step, waste liquid in incubation slot is discharged in waste liquid bottle by the work of peristaltic pump, end-use device can be dried processing to film bar automatically, after completing, film bar is carried out to automatic interpretation.Described equipment adopts the experimental situation of sealing, can protect operating personnel to avoid the threat of potential infectious clinical sample, during operation, without on duty, detect and finishes rear automatic buzzing prompting.
Described in the present patent application, advantage and the good effect of full-automatic immunoblotting assay instrument are:
1. the disposable use pickup groove of separate single person-portion is placed in the present invention's new design on shaking table, workbench for steps such as the hatching of Western blot sample, washing lotion, combination, substrates, disposable use, avoids the reliability of cross pollution and Western blot sample between sample.And the existing dish of hatching of putting disposable a plurality of grooves position that is mostly, not finishing just needs to discard, thus the waste causing;
2. the present invention has the function of film bar auto-drying, by regulating wind speed, temperature and drying time, makes film bar under drying regime, carry out interpretation, guarantees the accuracy of result.And prior art is all without this function, need to take out film bar naturally to dry or blot with thieving paper, be not easy to operation;
3. the present invention has film bar automatic interpretation function, by the camera collection film bar image of high definition, through software, analyzing is appearred in image, draws result accurately.And prior art is for being used scanner or special-purpose interpretoscope, need to support the use with Western blot instrument inconvenient operation;
4. the present invention has realized under the washing of film bar, oven dry and interpretation one and having operated, the pollution of having avoided prior art film bar to be dried and having needed contact membranes bar to cause during interpretation.
Accompanying drawing explanation
Fig. 1 is the one-piece construction explosive view of the present patent application embodiment;
Fig. 2 is the structure explosive view of the present patent application embodiment bracing or strutting arrangement;
Fig. 3 is the structure explosive view of the present patent application embodiment peristaltic-type pump arrangement;
Fig. 4 is the structure explosive view of the present patent application embodiment shaking table device;
Fig. 5 is the structure explosive view of the present patent application embodiment interpretation device;
Fig. 6 is the dry structure explosive view with adding liquid sucking device of the present patent application embodiment;
Fig. 7 is schematic diagram after the overall package of the present patent application embodiment;
Fig. 8 is the operational flowchart of the present patent application;
Wherein, 1 is casing, 2 is bracing or strutting arrangement, 3 is peristaltic-type pump arrangement, 4 is shaking table device, 5 is interpretation device, 6 for being dried and adding liquid sucking device, 11 is front shroud, 12 is side plate, 13 is balustrade panel, 20 is shell, 201 is front apron, 202 is backboard, 203 is side back up pad, 204 is upper backup pad, 205 is lower supporting plate, 206 is drain bar, 21 is stepper motor, 22 is synchronous belt, 23 is the first fixed block, 24 is the second fixed block, 25 is slide rail, 26 is drag chain, 31 is support, 32 is reagent bottle bracket, 33 is pump support, 34 is peristaltic pump, 35 is fan, 40 is swing motor, 41 is left socle, 42 is right support, 43 is connecting rod, 44 is link supports frame, 45 is tray rack, 450 is board slot, 46 is left support frame, 47 is right support frame, 48 is side cover plate, 49 is waste liquid pool, 490 for there being waste liquid pool cover plate, 50 is camera, 51 is lens supporting structure, 52 is light source fixed head, 53 is top cover, 54 is upper cover, 55 is protecgulum, 56 is lower cover, 57 is side cover, 60 is motor, 61 is fan, 62 is heating plate, 63 is guide rail fixed plate, 64 is guide rail, 65 is the main fixed head of pipe, 66 is the main fixed head of module, 67 is front shroud, 68 is left cover, 69 is right cover plate, 70 is bracing frame, 71 is module main supporting plate, 72 is pipe banking stop.
Embodiment
Below in conjunction with specific embodiment, the technology contents described in the present patent application is carried out to nonrestrictive explanation and explanation, object is to understand better described technology contents for the public.
As shown in Figure 1, full-automatic immunoblotting assay instrument described in the present patent application, comprise casing 1, casing comprises base plate, postnotum, two side plates 12 and front shroud 11, front shroud 11 and two side plates 12 are hinged, can realize opening and closing, the front end of front shroud 11 is also provided with the balustrade panel 13 of convenient operation, in casing, be provided with control device, bracing or strutting arrangement 2, peristaltic-type pump arrangement 3, shaking table device 4, be dried and add liquid sucking device 6 and interpretation device 5, control device comprises power supply and control circuit board, control work and the operation of whole analyser each several part element and module, bracing or strutting arrangement 2 is arranged on casing 1 inside, comprise shell 20 and the synchronous belt 22 that is arranged on the stepper motor 21 in shell 20 and is driven by stepper motor 21, synchronous belt 22 drives the first fixed block 23 and the second fixed block 24, the first fixed block 23 drives and is dried and adds liquid sucking device 6, the second fixed block 24 drives interpretation device 5, the shell 20 of bracing or strutting arrangement 2 comprises front apron 201, backboard 202, two side back up pads 203 of both sides are set, and be arranged on, the upper backup pad 204 of lower both sides and lower supporting plate 205, in shell 20, be also provided with slide rail 25, the first fixed block 23 and the second fixed block 24 slide along slide rail 25 under the drive of synchronous belt 22, front apron 201 is provided with strip opening, the first fixed block and 23 second fixed blocks 24 expose to described strip opening, described support dress, 2 also comprise the drain bar 206 being arranged on shell 20, for receiving the liquid being spilt by peristaltic pump, also be provided with in the enclosure for the fixing drag chain 26 of wire rod, peristaltic-type pump arrangement 3 is positioned on bracing or strutting arrangement, comprise support 31 and be arranged on the reagent bottle bracket 32 with a plurality of reagent bottle fixed orifices on support 31, support 31 rears are provided with pump support 33, a plurality of peristaltic pumps 34 are installed on pump support 33, each peristaltic pump 34 stretches in reagent bottle by flexible pipe respectively, is also provided with the fan 35 for dispelling the heat in support 31, shaking table device 4 arranges bracing or strutting arrangement 3 front lower places, the tray rack 45 that comprises swing motor 40 and driven by swing motor 40, and tray rack 45 is provided with a plurality of board slots 450 that detect film bar that hold, and tray rack 45 is driven and is waved by transmission shaft by swing motor 40, shaking table device 4 also comprise left socle 41, right support 42 and be arranged on left socle 41 and right support 42 between connecting rod 43, tray rack 45 is fixed on connecting rod 43 by several link supports framves 44, thereby swing motor 40 drives left socle 41 or right support 42 to drive tray rack 45 to wave, on the support of left and right, be respectively equipped with left support frame 46 and right support frame 47, on tray rack 45 1 sides edges, be provided with side cover plate 48, shaking table device 4 also comprise be arranged on tray rack 45 1 sides for storing the waste liquid pool 49 that detects waste liquid, waste liquid pool 49 is provided with waste liquid pool cover plate 490, interpretation device 5 is positioned at the top of tray rack 45, and be connected with the synchronous belt 22 of bracing or strutting arrangement by the second fixed block 24, comprise camera 50, light source and the load module that the data input computing machine collecting is processed, interpretation device 5 comprises interpretation crust of the device, interpretation crust of the device comprises two side covers 57 of top cover 53, protecgulum 54, upper cover 55,56 lower covers and both sides, camera 50 is fixed on top cover 53 inwalls by lens supporting structure 51, described light source is LED chip, and LED chip is arranged on light source fixed head 52, be dried and add the top that liquid sucking device 6 is positioned at tray rack 45, and be connected with the synchronous belt 22 of bracing or strutting arrangement by the first fixed block 23, comprise the drying device that one is installed and add liquid sucking device, comprise front shroud 67, left cover 68, right cover plate 69, the fan 61 that drying device comprises motor 60 and driven by motor 60, fan 61 belows are provided with heating plate 62, add liquid sucking device and comprise the guide rail 64 with slide block, guide rail 64 is fixed in guide rail fixed plate 63, guide rail pipe fixed head 63 is fixed on the main fixed head 66 of module, guide rail 64 is provided with for fixedly adding the main fixed head 65 of pipe of pipette, described device also comprises for fixing described dry bracing frame 70 and module main supporting plate 71 with adding liquid sucking device, module main supporting plate 71 is provided with pipe banking stop 72.
The operation of the full-automatic immunoblotting assay instrument described in the present patent application and using method and step as shown in Figure 8, repeat no more here.
Full-automatic immunoblotting assay instrument described in the present patent application, is equipped with Intelligent Chinese-character function software easy to learn, and the weak positive quality control band on can binding reagents product is exported semiquantitative sentence read result; And there is powerful patient information management system, meet the requirement of laboratory comprehensive informationization management, detecting data files automatically, adopt modular design, each module (application of sample, hatch, rinse, scan) all can operate separately, and has auto-drying function, circulating image analysis, report template, with original membrane bar scanned copy, guarantees that result is really reliable.
Should be clear and definite be; above-mentioned embodiment should not be construed as the restriction to technical scheme described in the present patent application; in fact; all with the whole and each several part improvement to described analyser of identical or approximate principle; so comprise the arrangement position of its shape, size material, each several part element and module; and identical function element be equal to replacement, take and realize basic identical technique effect as object, all within the present patent application technical scheme required for protection.
Claims (9)
1. a full-automatic immunoblotting assay instrument, is characterized in that: comprises casing, in casing, is provided with control device, bracing or strutting arrangement, peristaltic-type pump arrangement, shaking table device, dry and add liquid sucking device and interpretation device,
-control device comprises power supply and control circuit board;
-bracing or strutting arrangement is arranged on casing inside, comprise shell and stepper motor in the enclosure and the synchronous belt being driven by stepper motor are set, synchronous belt drives the first fixed block and the second fixed block, and the first fixed block drives dry and adds liquid sucking device, and the second fixed block drives interpretation device;
-peristaltic-type pump arrangement is positioned on bracing or strutting arrangement, comprise support and be arranged on the reagent bottle bracket with a plurality of reagent bottle fixed orifices on support, support rear is provided with pump support, and a plurality of peristaltic pumps are installed on pump support, and each peristaltic pump stretches in reagent bottle by flexible pipe respectively;
-shaking table device arranges bracing or strutting arrangement front lower place, comprises swing motor and the tray rack being driven by swing motor, and tray rack is provided with a plurality of board slots that detect film bar that hold, and tray rack is driven and waved by transmission shaft by swing motor;
-be dried and add the top that liquid sucking device is positioned at tray rack, and be connected with the synchronous belt of bracing or strutting arrangement by the first fixed block, comprise the drying device that one is installed and add liquid sucking device, drying device comprises motor and by the fan of driven by motor, fan below is provided with heating plate, adds liquid sucking device and comprises the guide rail with slide block, and guide rail is fixed in guide rail fixed plate, guide rail pipe fixed head is fixed on the main fixed head of module, and guide rail is provided with for fixedly adding the main fixed head of pipe of pipette;
-interpretation device is positioned at the top of tray rack, and is connected with the synchronous belt of bracing or strutting arrangement by the second fixed block, comprises camera, light source and the load module that the data input computing machine collecting is processed.
2. full-automatic immunoblotting assay instrument according to claim 1, it is characterized in that: described casing comprises base plate, postnotum, two side plates and front shroud, front shroud and two side plates are hinged, can realize opening and closing, and the front end of front shroud is also provided with the balustrade panel of convenient operation.
3. full-automatic immunoblotting assay instrument according to claim 1 and 2, it is characterized in that: the shell of described bracing or strutting arrangement comprises front apron, backboard, two side back up pads of both sides are set, and the upper backup pad and the lower supporting plate that are arranged on upper and lower both sides, in shell, be also provided with slide rail, the first fixed block and the second fixed block slide along slide rail under the drive of synchronous belt, front apron is provided with strip opening, and the first fixed block and the second fixed block expose to described strip opening.
4. full-automatic immunoblotting assay instrument according to claim 3, is characterized in that: described bracing or strutting arrangement also comprises the drain bar being arranged on shell, for receiving the liquid being spilt by peristaltic pump, is also provided with in the enclosure for the fixing drag chain of wire rod.
5. full-automatic immunoblotting assay instrument according to claim 1 and 2, is characterized in that: described peristaltic-type pump arrangement also comprises the fan for dispelling the heat being arranged in support.
6. full-automatic immunoblotting assay instrument according to claim 1 and 2, it is characterized in that: described shaking table device also comprise left socle, right support and be arranged on left socle and right support between connecting rod, tray rack is fixed on connecting rod by several link supports framves, thereby swing motor drives left socle or right support to drive tray rack to wave, on the support of left and right, be respectively equipped with left support frame and right support frame, on tray rack one side edge, be provided with side cover plate.
7. full-automatic immunoblotting assay instrument according to claim 6, is characterized in that: described shaking table device also comprise be arranged on tray rack one side for storing the waste liquid pool that detects waste liquid, waste liquid pool is provided with waste liquid pool cover plate.
8. full-automatic immunoblotting assay instrument according to claim 1 and 2, it is characterized in that: described interpretation device comprises interpretation crust of the device, interpretation crust of the device comprises two side covers of top cover, upper cover, lower cover, protecgulum and both sides, camera is fixed on top cover inwall by lens supporting structure, described light source is LED chip, and LED chip is arranged on light source fixed head.
9. full-automatic immunoblotting assay instrument according to claim 1 and 2, it is characterized in that: described dry with add that liquid sucking device also comprises left cover, right cover plate, front shroud and for fixing described dry bracing frame and module main supporting plate with adding liquid sucking device, module main supporting plate is provided with pipe banking stop.
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| CN201310689074.6A CN103616526B (en) | 2013-12-16 | 2013-12-16 | A kind of full-automatic immunoblotting assay instrument and using method thereof and application |
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Cited By (14)
| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103954785A (en) * | 2014-03-20 | 2014-07-30 | 欧蒙医学诊断（中国）有限公司 | Liquid system of western-blot analyzer |
| CN104007267A (en) * | 2014-03-20 | 2014-08-27 | 欧蒙医学诊断（中国）有限公司 | Swing system of protein blotting analyzer |
| CN104007270A (en) * | 2014-03-20 | 2014-08-27 | 欧蒙医学诊断（中国）有限公司 | Biological detection analysis apparatus |
| CN104155461A (en) * | 2014-08-14 | 2014-11-19 | 欧蒙医学诊断（中国）有限公司 | Biological detection analysis equipment with air drying module and usage method thereof |
| CN105628936A (en) * | 2016-03-22 | 2016-06-01 | 苏州捷美电子有限公司 | Western Blot laboratory instrument |
| CN106680514A (en) * | 2017-03-27 | 2017-05-17 | 武汉优视科技有限公司 | Automatic detector for immunoblotting |
| CN106733886A (en) * | 2016-12-20 | 2017-05-31 | 上海长海医院 | A kind of full automatic high efficiency western blot wash membranous system |
| CN108120840A (en) * | 2018-01-19 | 2018-06-05 | 深圳市亚辉龙生物科技股份有限公司 | Full-automatic immunoblotting assay instrument |
| CN108241063A (en) * | 2016-12-26 | 2018-07-03 | 兰州奥润环保设备有限公司 | Double diaphragm Western blot instrument and its Examination on experimental operation |
| CN108241062A (en) * | 2016-12-26 | 2018-07-03 | 兰州奥润环保设备有限公司 | Multimembrane piece Western blot instrument and its Examination on experimental operation |
| CN108802403A (en) * | 2018-07-28 | 2018-11-13 | 湖南中瑞互信医疗科技有限公司 | A kind of constant-temperature incubation shaking table |
| CN109100502A (en) * | 2018-07-28 | 2018-12-28 | 湖南中瑞互信医疗科技有限公司 | A kind of full-automatic immunoblotting instrument |
| TWI804766B (en) * | 2020-10-27 | 2023-06-11 | 國立臺灣大學 | Apparatus for accelerating and performing uniformly reaction of to-be-reacted substances and reactants contained in wettable substrate, quickly probing the to-be-reacted substances system containing the apparatus, and coater |
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