CN103609872A - Mink acidified daily ration additive capable of reducing nitrogen discharge and phosphorus discharge - Google Patents
Mink acidified daily ration additive capable of reducing nitrogen discharge and phosphorus discharge Download PDFInfo
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- CN103609872A CN103609872A CN201310497418.3A CN201310497418A CN103609872A CN 103609872 A CN103609872 A CN 103609872A CN 201310497418 A CN201310497418 A CN 201310497418A CN 103609872 A CN103609872 A CN 103609872A
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- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 title abstract description 30
- 241000772415 Neovison vison Species 0.000 title abstract description 28
- OAICVXFJPJFONN-UHFFFAOYSA-N Phosphorus Chemical compound [P] OAICVXFJPJFONN-UHFFFAOYSA-N 0.000 title abstract description 26
- 229910052698 phosphorus Inorganic materials 0.000 title abstract description 26
- 239000011574 phosphorus Substances 0.000 title abstract description 26
- 229910052757 nitrogen Inorganic materials 0.000 title abstract description 15
- 239000000654 additive Substances 0.000 title abstract description 12
- 230000000996 additive effect Effects 0.000 title abstract description 10
- 230000000694 effects Effects 0.000 abstract description 12
- 230000007613 environmental effect Effects 0.000 abstract description 12
- IMQLKJBTEOYOSI-GPIVLXJGSA-N Inositol-hexakisphosphate Chemical compound OP(O)(=O)O[C@H]1[C@H](OP(O)(O)=O)[C@@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@H](OP(O)(O)=O)[C@@H]1OP(O)(O)=O IMQLKJBTEOYOSI-GPIVLXJGSA-N 0.000 abstract description 10
- 235000002949 phytic acid Nutrition 0.000 abstract description 10
- KRKNYBCHXYNGOX-UHFFFAOYSA-N citric acid Chemical compound OC(=O)CC(O)(C(O)=O)CC(O)=O KRKNYBCHXYNGOX-UHFFFAOYSA-N 0.000 abstract description 9
- 206010012735 Diarrhoea Diseases 0.000 abstract description 7
- YBJHBAHKTGYVGT-ZKWXMUAHSA-N (+)-Biotin Chemical compound N1C(=O)N[C@@H]2[C@H](CCCCC(=O)O)SC[C@@H]21 YBJHBAHKTGYVGT-ZKWXMUAHSA-N 0.000 abstract description 6
- GHOKWGTUZJEAQD-ZETCQYMHSA-N (D)-(+)-Pantothenic acid Chemical compound OCC(C)(C)[C@@H](O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-ZETCQYMHSA-N 0.000 abstract description 6
- OVBPIULPVIDEAO-LBPRGKRZSA-N folic acid Chemical compound C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)N[C@@H](CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-LBPRGKRZSA-N 0.000 abstract description 6
- 210000001035 gastrointestinal tract Anatomy 0.000 abstract description 6
- 235000018102 proteins Nutrition 0.000 abstract description 6
- 102000004169 proteins and genes Human genes 0.000 abstract description 6
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- 241000588724 Escherichia coli Species 0.000 abstract description 5
- 240000008042 Zea mays Species 0.000 abstract description 5
- 235000005824 Zea mays ssp. parviglumis Nutrition 0.000 abstract description 5
- 235000002017 Zea mays subsp mays Nutrition 0.000 abstract description 5
- 230000008901 benefit Effects 0.000 abstract description 5
- 235000005822 corn Nutrition 0.000 abstract description 5
- 230000029087 digestion Effects 0.000 abstract description 5
- 229940088598 enzyme Drugs 0.000 abstract description 5
- 108010011619 6-Phytase Proteins 0.000 abstract description 4
- 238000004519 manufacturing process Methods 0.000 abstract description 4
- 235000019629 palatability Nutrition 0.000 abstract description 4
- 229940085127 phytase Drugs 0.000 abstract description 4
- 239000000843 powder Substances 0.000 abstract description 4
- GHOKWGTUZJEAQD-UHFFFAOYSA-N Chick antidermatitis factor Natural products OCC(C)(C)C(O)C(=O)NCCC(O)=O GHOKWGTUZJEAQD-UHFFFAOYSA-N 0.000 abstract description 3
- 108010068370 Glutens Proteins 0.000 abstract description 3
- OVBPIULPVIDEAO-UHFFFAOYSA-N N-Pteroyl-L-glutaminsaeure Natural products C=1N=C2NC(N)=NC(=O)C2=NC=1CNC1=CC=C(C(=O)NC(CCC(O)=O)C(O)=O)C=C1 OVBPIULPVIDEAO-UHFFFAOYSA-N 0.000 abstract description 3
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 abstract description 3
- 108010059820 Polygalacturonase Proteins 0.000 abstract description 3
- 229960002685 biotin Drugs 0.000 abstract description 3
- 235000020958 biotin Nutrition 0.000 abstract description 3
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- 108010093305 exopolygalacturonase Proteins 0.000 abstract description 3
- 229960000304 folic acid Drugs 0.000 abstract description 3
- 235000019152 folic acid Nutrition 0.000 abstract description 3
- 239000011724 folic acid Substances 0.000 abstract description 3
- 235000021312 gluten Nutrition 0.000 abstract description 3
- 230000006872 improvement Effects 0.000 abstract description 3
- 235000001968 nicotinic acid Nutrition 0.000 abstract description 3
- 229960003512 nicotinic acid Drugs 0.000 abstract description 3
- 239000011664 nicotinic acid Substances 0.000 abstract description 3
- 235000021238 nutrient digestion Nutrition 0.000 abstract description 3
- 230000003647 oxidation Effects 0.000 abstract description 3
- 238000007254 oxidation reaction Methods 0.000 abstract description 3
- 229940055726 pantothenic acid Drugs 0.000 abstract description 3
- 235000019161 pantothenic acid Nutrition 0.000 abstract description 3
- 239000011713 pantothenic acid Substances 0.000 abstract description 3
- 230000033228 biological regulation Effects 0.000 abstract description 2
- OEYIOHPDSNJKLS-UHFFFAOYSA-N choline Chemical compound C[N+](C)(C)CCO OEYIOHPDSNJKLS-UHFFFAOYSA-N 0.000 abstract description 2
- 229960001231 choline Drugs 0.000 abstract description 2
- 238000004925 denaturation Methods 0.000 abstract description 2
- 230000036425 denaturation Effects 0.000 abstract description 2
- 230000036541 health Effects 0.000 abstract description 2
- 238000005457 optimization Methods 0.000 abstract description 2
- 238000004321 preservation Methods 0.000 abstract description 2
- 230000035755 proliferation Effects 0.000 abstract description 2
- 239000000126 substance Substances 0.000 abstract description 2
- 230000000968 intestinal effect Effects 0.000 abstract 1
- 101150051135 Mink1 gene Proteins 0.000 description 22
- 241001465754 Metazoa Species 0.000 description 13
- 235000019621 digestibility Nutrition 0.000 description 12
- IMQLKJBTEOYOSI-UHFFFAOYSA-N Phytic acid Natural products OP(O)(=O)OC1C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C(OP(O)(O)=O)C1OP(O)(O)=O IMQLKJBTEOYOSI-UHFFFAOYSA-N 0.000 description 8
- 238000010521 absorption reaction Methods 0.000 description 7
- 235000015097 nutrients Nutrition 0.000 description 7
- 239000000467 phytic acid Substances 0.000 description 7
- 229940068041 phytic acid Drugs 0.000 description 7
- 238000009395 breeding Methods 0.000 description 6
- 230000001488 breeding effect Effects 0.000 description 6
- 241000196324 Embryophyta Species 0.000 description 5
- 102000057297 Pepsin A Human genes 0.000 description 5
- 108090000284 Pepsin A Proteins 0.000 description 5
- 239000003242 anti bacterial agent Substances 0.000 description 5
- 229940088710 antibiotic agent Drugs 0.000 description 5
- 230000037396 body weight Effects 0.000 description 5
- 229940111202 pepsin Drugs 0.000 description 5
- 241000894006 Bacteria Species 0.000 description 4
- 239000002535 acidifier Substances 0.000 description 4
- 229940095602 acidifiers Drugs 0.000 description 4
- 235000005911 diet Nutrition 0.000 description 4
- 239000003925 fat Substances 0.000 description 4
- OYPRJOBELJOOCE-UHFFFAOYSA-N Calcium Chemical compound [Ca] OYPRJOBELJOOCE-UHFFFAOYSA-N 0.000 description 3
- 235000019750 Crude protein Nutrition 0.000 description 3
- 241000282339 Mustela Species 0.000 description 3
- 239000002253 acid Substances 0.000 description 3
- 230000000433 anti-nutritional effect Effects 0.000 description 3
- 239000011575 calcium Substances 0.000 description 3
- 229910052791 calcium Inorganic materials 0.000 description 3
- 235000001465 calcium Nutrition 0.000 description 3
- 125000002915 carbonyl group Chemical group [*:2]C([*:1])=O 0.000 description 3
- 230000037213 diet Effects 0.000 description 3
- 235000019197 fats Nutrition 0.000 description 3
- 238000003756 stirring Methods 0.000 description 3
- 238000003860 storage Methods 0.000 description 3
- 238000011282 treatment Methods 0.000 description 3
- 241000287828 Gallus gallus Species 0.000 description 2
- 229920002472 Starch Polymers 0.000 description 2
- 239000002585 base Substances 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 2
- 235000019784 crude fat Nutrition 0.000 description 2
- 230000006866 deterioration Effects 0.000 description 2
- 239000003344 environmental pollutant Substances 0.000 description 2
- 235000013305 food Nutrition 0.000 description 2
- 150000004676 glycans Chemical class 0.000 description 2
- 239000004615 ingredient Substances 0.000 description 2
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- 230000004060 metabolic process Effects 0.000 description 2
- 231100000719 pollutant Toxicity 0.000 description 2
- 229920001282 polysaccharide Polymers 0.000 description 2
- 239000005017 polysaccharide Substances 0.000 description 2
- 235000019698 starch Nutrition 0.000 description 2
- 239000008107 starch Substances 0.000 description 2
- 210000002784 stomach Anatomy 0.000 description 2
- 241000251468 Actinopterygii Species 0.000 description 1
- 229910021580 Cobalt(II) chloride Inorganic materials 0.000 description 1
- 208000018522 Gastrointestinal disease Diseases 0.000 description 1
- 229910003424 Na2SeO3 Inorganic materials 0.000 description 1
- 241000700159 Rattus Species 0.000 description 1
- 235000019764 Soybean Meal Nutrition 0.000 description 1
- 230000002378 acidificating effect Effects 0.000 description 1
- 239000003513 alkali Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000003975 animal breeding Methods 0.000 description 1
- 235000021052 average daily weight gain Nutrition 0.000 description 1
- 235000014590 basal diet Nutrition 0.000 description 1
- 210000000941 bile Anatomy 0.000 description 1
- 230000008859 change Effects 0.000 description 1
- 210000004913 chyme Anatomy 0.000 description 1
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 description 1
- 229910000366 copper(II) sulfate Inorganic materials 0.000 description 1
- 230000003247 decreasing effect Effects 0.000 description 1
- 230000000378 dietary effect Effects 0.000 description 1
- 102000038379 digestive enzymes Human genes 0.000 description 1
- 108091007734 digestive enzymes Proteins 0.000 description 1
- 239000003640 drug residue Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 235000021050 feed intake Nutrition 0.000 description 1
- 230000002496 gastric effect Effects 0.000 description 1
- 230000003803 hair density Effects 0.000 description 1
- 230000005764 inhibitory process Effects 0.000 description 1
- BAUYGSIQEAFULO-UHFFFAOYSA-L iron(2+) sulfate (anhydrous) Chemical compound [Fe+2].[O-]S([O-])(=O)=O BAUYGSIQEAFULO-UHFFFAOYSA-L 0.000 description 1
- 229910000359 iron(II) sulfate Inorganic materials 0.000 description 1
- 210000004185 liver Anatomy 0.000 description 1
- 230000007774 longterm Effects 0.000 description 1
- 238000012423 maintenance Methods 0.000 description 1
- SQQMAOCOWKFBNP-UHFFFAOYSA-L manganese(II) sulfate Chemical compound [Mn+2].[O-]S([O-])(=O)=O SQQMAOCOWKFBNP-UHFFFAOYSA-L 0.000 description 1
- 229910000357 manganese(II) sulfate Inorganic materials 0.000 description 1
- 239000000463 material Substances 0.000 description 1
- 238000000034 method Methods 0.000 description 1
- 244000000010 microbial pathogen Species 0.000 description 1
- 244000005706 microflora Species 0.000 description 1
- 239000000203 mixture Substances 0.000 description 1
- 231100000989 no adverse effect Toxicity 0.000 description 1
- 235000015816 nutrient absorption Nutrition 0.000 description 1
- 230000001590 oxidative effect Effects 0.000 description 1
- 150000002978 peroxides Chemical class 0.000 description 1
- 235000014786 phosphorus Nutrition 0.000 description 1
- 244000062645 predators Species 0.000 description 1
- 230000006318 protein oxidation Effects 0.000 description 1
- 239000002994 raw material Substances 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 150000003839 salts Chemical class 0.000 description 1
- 235000015921 sodium selenite Nutrition 0.000 description 1
- 239000011781 sodium selenite Substances 0.000 description 1
- 239000004455 soybean meal Substances 0.000 description 1
- 239000002699 waste material Substances 0.000 description 1
- 235000019786 weight gain Nutrition 0.000 description 1
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 description 1
- 229910000368 zinc sulfate Inorganic materials 0.000 description 1
- 239000011686 zinc sulphate Substances 0.000 description 1
- 235000009529 zinc sulphate Nutrition 0.000 description 1
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- Fodder In General (AREA)
Abstract
本发明公开一种降低氮磷排放的水貂酸化日粮添加剂,它每千克含有:柠檬酸450-550g,植酸酶75000-12500IU,果胶酶80000-120000IU,VA1000000-2000000IU,VD32000000-3000000IU,VE6000-9000IU,VB1600-900mg,VB2800-1200mg,VB6300-450mg,VB1212-15mg,VK3100-150mg,VC40000-60000mg,烟酸4000-6000mg,泛酸1200-1500mg,生物素20-30mg,叶酸80-120mg,胆碱30000-45000mg,FeSO422000-28200mg,CuSO41500-2000mg,MnSO43500-5000mg,ZnSO47500-9000mg,KI60-90mg,Na2SeO330-40mg,CoCl21700-2000mg,玉米蛋白粉补足至1000g。该添加剂可调节饲料pH值,提高适口性;调控水貂胃肠道pH值、酶活、促进小肠绒毛发育,提高营养样物质消化利用率和生产性能;可显著抑制鲜料大肠杆菌增殖、延缓水貂饲料脂肪氧化和蛋白质变性,降低夏季水貂腹泻率;提高磷特别是植酸磷的利用率,显著降低氮、磷环境排放量。实现饲料保质、消化道环境调控、酶活提高、优化营养物质消化利用,改善水貂健康状况,提高经济和环境效益。The invention discloses an acidified ration additive for mink that reduces nitrogen and phosphorus emissions, which contains per kilogram: citric acid 450-550g, phytase 75000-12500IU, pectinase 80000-120000IU, VA1000000-2000000IU, VD 3 2000000-3000000IU , VE6000-9000IU, VB 1 600-900mg, VB 2 800-1200mg, VB 6 300-450mg, VB 12 12-15mg, VK 3 100-150mg, VC40000-60000mg, niacin 4000-6000mg, pantothenic acid 1200-1500mg, Biotin 20-30mg, folic acid 80-120mg, choline 30000-45000mg, FeSO 4 22000-28200mg, CuSO 4 1500-2000mg, MnSO 4 3500-5000mg, ZnSO 4 7500-9000mg, KI60-90mg, Na 2 SeO 3 30 -40mg, CoCl 2 1700-2000mg, corn gluten powder supplemented to 1000g. The additive can adjust the pH value of the feed and improve the palatability; regulate the pH value and enzyme activity of the gastrointestinal tract of mink, promote the development of small intestinal villi, improve the digestion and utilization rate of nutrient-like substances and production performance; it can significantly inhibit the proliferation of E. coli in fresh feed and delay the development of mink Feed fat oxidation and protein denaturation can reduce the summer mink diarrhea rate; increase the utilization rate of phosphorus, especially phytate phosphorus, and significantly reduce nitrogen and phosphorus environmental emissions. Realize feed quality preservation, digestive tract environment regulation, enzyme activity improvement, optimization of nutrient digestion and utilization, improve mink health, and increase economic and environmental benefits.
Description
技术领域 technical field
本发明涉及饲料工业中添加剂配方技术领域,特别是涉及一种降低氮磷排放的水貂酸化日粮添加剂,属于毛皮动物饲养和饲料加工技术领域。 The invention relates to the technical field of additive formula in the feed industry, in particular to an acidified ration additive for mink that reduces nitrogen and phosphorus discharge, and belongs to the technical field of fur animal breeding and feed processing.
背景技术 Background technique
随着生活水平的提高,人们对食品安全和环境保护的重视程度也越来越高,由于“超级菌”的诞生和畜产品中药物残留的问题的影响,部分国家已经禁止在饲料中使用抗生素,我国也陆续禁止了部分抗生素的使用,并对允许使用的抗生素严格规定了使用剂量、范围以及停药期。面对新的形势,“低抗”、“无抗”技术和产品越来越受重视。消化道酸性环境是饲料组分在动物体内被充分消化吸收、有益菌群合理生长、病原微生物受到有效抑制的必要条件,在饲料中添加酸化剂是调节胃内pH的有效手段。酸化剂作为一种绿色添加剂,能降低动物肠胃pH值,提高消化酶活性,改善消化道微生物区系,抑制有害菌生长,促进有益菌繁殖(E.Mani-López等2012), 延缓脂肪和蛋白质氧化变质(王杏珠等1993),促进营养物质吸收、提高动物生长的性能(Pastuszewska等2007)、(Hashemi等2012),在抗生素受到更多的限制和环境保护日益受到重视的状况下,必将得到越来越广泛的应用。大量研究表明,添加酸化剂能够改善日粮适口性,提高采食量和营养物质利用率(张宜辉等,2013;张玲等,2013;连凯霞等,2013;朱剑锋等,2013)。在国外,尤其是禁用抗生素的欧洲国家,酸化剂已经作为常规饲料原料,被广泛应用到动物饲料中。 With the improvement of living standards, people pay more and more attention to food safety and environmental protection. Due to the birth of "super bacteria" and the influence of drug residues in animal products, some countries have banned the use of antibiotics in feed , my country has also banned the use of some antibiotics one after another, and strictly stipulated the dosage, scope and withdrawal period of the antibiotics that are allowed to be used. Facing the new situation, "low resistance" and "no resistance" technologies and products are getting more and more attention. The acidic environment of the digestive tract is a necessary condition for the full digestion and absorption of feed components in animals, the reasonable growth of beneficial bacteria, and the effective inhibition of pathogenic microorganisms. Adding acidifiers to feed is an effective means to adjust the pH in the stomach. As a green additive, acidifiers can reduce the pH value of animals’ stomachs, increase the activity of digestive enzymes, improve the microflora of the digestive tract, inhibit the growth of harmful bacteria, promote the reproduction of beneficial bacteria (E.Mani-López et al. 2012), delay the loss of fat and protein Oxidative deterioration (Wang Xingzhu et al. 1993), promote the absorption of nutrients, improve the performance of animal growth (Pastuszewska et al. 2007), (Hashemi et al. 2012), in the situation of more restrictions on antibiotics and increasing attention to environmental protection, it will definitely be obtained more and more widely used. A large number of studies have shown that adding acidifiers can improve the palatability of diets, increase feed intake and nutrient utilization (Zhang Yihui et al., 2013; Zhang Ling et al., 2013; Lian Kaixia et al., 2013; Zhu Jianfeng et al., 2013). In foreign countries, especially in European countries where antibiotics are banned, acidifiers have been widely used in animal feed as conventional feed ingredients.
磷是维持动物维持需要和生长发育的重要元素,但是植物性饲料原料中的日粮磷是以植酸(肌醇六磷酸,IP6)的形式存在。动物基本不能消化植酸,因此只能利用植物性原料中很少部分的磷。植酸也是一种抗营养因子,可以结合蛋白质和其他重要养分,使它们不能被动物利用,不仅降低饲料利用率和增加饲料成本,而且通过刺激肠道中过度的内源性分泌,导致动物能量和蛋白质/氨基酸的浪费(王亚妮,2013;李江长等,2013;司马博锋,2013;赵瑜等,2013;姚丽欣,2013;张建刚等,2013)。水貂属于肉食动物,体内缺乏分解非淀粉多糖的酶,导致其对植物性饲料的消化能力比一般动物较差,玉米、豆粕等植物性饲料原料中含有较多的非淀粉多糖和植酸,是水貂等单胃动物主要的抗营养因子,还能干扰日粮中其他组分的营养消化吸收和利用,从而导致饲料营养成分的消化利用率下降,并造成水貂胃肠疾病等负面影响(何玉华,等,2009;梁玉嬉,金春英,李福军,2013)。植酸酶可降解植酸,解除植酸的抗营养作用,释放有机磷,增加动物体对植物中磷的吸收利用率,从而减少无机磷的添加,降低磷排放(雷桥波,2010)。 Phosphorus is an important element to maintain animal maintenance needs and growth and development, but dietary phosphorus in plant-based feed ingredients exists in the form of phytic acid (phytic acid, IP6). Animals basically cannot digest phytic acid, so they can only use a small part of the phosphorus in plant raw materials. Phytic acid is also an anti-nutritional factor that can bind protein and other important nutrients and make them unavailable to animals, which not only reduces feed utilization and increases feed costs, but also leads to animal energy and Protein/amino acid waste (Wang Yani, 2013; Li Jiangchang et al., 2013; Sima Bofeng, 2013; Zhao Yu et al., 2013; Yao Lixin, 2013; Zhang Jiangang et al., 2013). Mink is a carnivore. It lacks the enzymes to decompose non-starch polysaccharides in its body, so its ability to digest plant feed is poorer than that of ordinary animals. Plant feed materials such as corn and soybean meal contain more non-starch polysaccharides and phytic acid. The main anti-nutritional factors of mink and other monogastric animals can also interfere with the nutrient digestion, absorption and utilization of other components in the diet, resulting in a decrease in the digestibility and utilization of feed nutrients and negative effects such as gastrointestinal diseases in mink (He Yuhua, et al., 2009; Liang Yuxi, Jin Chunying, Li Fujun, 2013). Phytase can degrade phytic acid, remove the anti-nutritional effect of phytic acid, release organic phosphorus, and increase the absorption and utilization of phosphorus in plants by animals, thereby reducing the addition of inorganic phosphorus and reducing phosphorus emissions (Lei Qiaobo, 2010).
发明内容 Contents of the invention
为了延长水貂鲜饲料保存时间,提高水貂营养物质消化率和生产性能,提高磷特别是植酸磷的吸收利用率,降低氮、磷等养殖污染物环境排放量,提高水貂养殖经济效益,在对前人研究和借鉴其他动物的酸化日粮和添加剂配方的基础上,根据水貂特性和生物学特点,以酸值(AV)、过氧化值(POV)、羰基值(COV)和挥发性盐基氮水平(TVBN)、大肠杆菌数、腹泻率、胃内食糜中胃蛋白酶活性;粗蛋白、粗脂肪、钙、磷的消化吸收率、皮重和皮长、毛绒密度和针、绒毛长度等重要客观指标为依据,研制出一种降低氮磷排放的水貂酸化日粮添加剂。 In order to prolong the storage time of mink fresh feed, improve the digestibility of mink nutrients and production performance, improve the absorption and utilization of phosphorus, especially phytate phosphorus, reduce the environmental discharge of nitrogen, phosphorus and other breeding pollutants, and improve the economic benefits of mink breeding, On the basis of previous studies and references to acidified diets and additive formulas of other animals, according to the characteristics and biological characteristics of mink, acid value (AV), peroxide value (POV), carbonyl value (COV) and volatile base Nitrogen level (TVBN), Escherichia coli number, diarrhea rate, pepsin activity in gastric chyme; digestion and absorption rate of crude protein, crude fat, calcium, phosphorus, tare weight and skin length, hair density and needle, hair length Based on important objective indicators such as , a mink acidified ration additive that reduces nitrogen and phosphorus emissions was developed.
该添加剂每千克各组分含量:柠檬酸450-550g,植酸酶75000-125000IU,果胶酶80000-120000IU,VA1000000-2000000IU,VD32000000-3000000IU,VE6000-9000IU,VB1600-900mg,VB2800-1200mg,VB6300-450mg,VB1212-15mg,VK3100-150mg,VC40000-60000mg,烟酸4000-6000mg,泛酸1200-1500mg,生物素20-30mg,叶酸80-120mg,胆碱30000-45000mg,FeSO422000-28200mg,CuSO41500-2000mg,MnSO4 3500-5000mg,ZnSO4 7500-9000mg,KI60-90mg,Na2SeO330-40mg,CoCl2 1700-2000mg,玉米蛋白粉补足至1000g。 The content of each component per kilogram of the additive: citric acid 450-550g, phytase 75000-125000IU, pectinase 80000-120000IU, VA1000000-2000000IU, VD 3 2000000-3000000IU, VE6000-9000IU, VB 1 600-900mg, VB 2 800-1200mg, VB 6 300-450mg, VB 12 12-15mg, VK 3 100-150mg, VC40000-60000mg, niacin 4000-6000mg, pantothenic acid 1200-1500mg, biotin 20-30mg, folic acid 80-120mg, bile Alkali 30000-45000mg, FeSO4 22000-28200mg, CuSO4 1500-2000mg, MnSO4 3500-5000mg , ZnSO4 7500-9000mg, KI60-90mg, Na2SeO3 30-40mg , CoCl2 1700-2000mg , corn gluten powder Make up to 1000g.
与现有技术相比,利用本发明可具有调节饲料PH值,提高适口性;调控水貂胃肠道PH值、酶活、提高营养样物质消化利用率、延缓水貂饲料脂肪氧化和蛋白质变性等功能,抑制鲜料大肠杆菌增殖、降低夏季水貂腹泻率;提高水貂营养物质消化率和生产性能;提高磷特别是植酸磷的吸收利用率,降低氮、磷等养殖污染物环境排放量。实现饲料保质、消化道环境调控、酶活提高、优化营养物质消化利用,改善水貂健康状况,使水貂养殖实现高效和环境友好有机结合,提高水貂养殖业经济和环境效益。 Compared with the prior art, the present invention can have the functions of adjusting feed pH value, improving palatability; regulating mink gastrointestinal tract pH value, enzyme activity, improving digestion and utilization rate of nutrient-like substances, delaying mink feed fat oxidation and protein denaturation, etc. , Inhibit the proliferation of Escherichia coli in fresh feed, reduce the diarrhea rate of minks in summer; improve the digestibility of nutrients and production performance of minks; improve the absorption and utilization of phosphorus, especially phytate phosphorus, and reduce the environmental emissions of nitrogen, phosphorus and other breeding pollutants. Realize feed quality preservation, digestive tract environmental regulation, enzyme activity improvement, optimization of nutrient digestion and utilization, improve the health of mink, make mink breeding realize the organic combination of high efficiency and environmental friendliness, and improve the economic and environmental benefits of mink breeding.
具体实施方式准确量取植酸酶1000000IU、果胶酶1000000IU、VA15000000IU、VD325000000IU、VE75000IU、VB17500mg、VB210000mg、VB64000mg、VB12130mg、VK31350mg、VC500000mg、烟酸50000mg、泛酸13500mg、生物素250mg、叶酸1000mg、胆碱400000mg、FeSO4250000mg、CuSO417500mg、MnSO4 45000mg、ZnSO4 85000mg、KI750mg、Na2SeO3350mg、CoCl2 18500mg,分别加入经过80目筛子玉米蛋白粉100g并搅拌均匀,将所有样品混合均匀后补加玉米蛋白粉至5000g后加5000g柠檬酸后搅拌均匀,分装、避光干燥保存。使用时按照饲料重量1%添加、搅拌均匀后,静置20-30分钟后使用。 Specific embodiments Accurately measure phytase 1000000IU, pectinase 1000000IU, VA15000000IU, VD 3 25000000IU, VE75000IU, VB 1 7500mg, VB 2 10000mg, VB 6 4000mg, VB 12 130mg, VK 3 000mg, niacin 5500mg, 00VC Pantothenic acid 13,500mg, biotin 250mg, folic acid 1,000mg, choline 400,000mg, FeSO 4 250,000mg, CuSO 4 17,500mg, MnSO 4 45,000mg, ZnSO 4 85,000mg, KI 750mg, Na 2 SeO 3 350mg , CoCl 2 18,500mg, respectively added through 80-mesh sieve Powder 100g and stir evenly, mix all samples evenly, add corn gluten powder to 5000g, add 5000g citric acid, stir evenly, subpackage, avoid light and dry storage. When using, add 1% of the feed weight, stir evenly, and let it stand for 20-30 minutes before using.
选择健康、体重相近、90日龄的雄性短毛黑水貂60只,平均体重1891.85±201.98g( ±SD),随机分成2组,每组30只,组间体重无显著差异(p>0.05)。以膨化玉米、海杂鱼、鸡肝、鸡骨架、食盐等配制基础日粮,以市场上常见的某添加剂为对照,饲养至育成期结束取皮。检测低温长期保存后的鲜饲料的酸值、羰基值、挥发性盐基氮值,不同时期大肠杆菌菌落数量和腹泻指数、腹泻率、采食后不同时间胃蛋白酶活性、干物质采食量、干物质消化率、粗蛋白消化率、氮代谢率、粗脂肪消化率、钙消化率、磷消化率、氮磷环境排放量、体增重、平均日增重、皮长、鲜皮重、针毛长度、绒毛长度等指标。 Select 60 healthy, 90-day-old male short-haired black mink with similar body weight, with an average body weight of 1891.85±201.98g ( ± SD ), were randomly divided into 2 groups, 30 rats in each group, and there was no significant difference in body weight between groups ( p > 0.05). The basal diet was prepared with puffed corn, sea fish, chicken liver, chicken skeleton, salt, etc., and a common additive in the market was used as a control, and the skin was removed from the breeding period. Detect the acid value, carbonyl value, volatile basic nitrogen value of fresh feed after long-term storage at low temperature, the number of E. coli colonies and diarrhea index, diarrhea rate, pepsin activity at different times after eating, dry matter intake, Dry matter digestibility, crude protein digestibility, nitrogen metabolism rate, crude fat digestibility, calcium digestibility, phosphorus digestibility, nitrogen and phosphorus environmental emissions, body weight gain, average daily weight gain, skin length, fresh skin weight, needle Hair length, fluff length and other indicators.
由表一可见,标志脂肪氧化指标的酸值和羰基值本实施例大大优于对照组,标志蛋白变化的挥发性盐基氮值也验证了这一点,添加本实施例后鲜料中大肠杆菌数下降趋势明显。由表二可见,添加本实施例后水貂腹泻指数和腹泻率随本实施例添加而下降趋势明显(P<0.01);干物质采食量无显著差异(p>0.05),均在150g左右,对生长发育期水貂体增重和平均日增重均有影响显著(P <0.05);干物质消化率、粗蛋白消化率、氮代谢率、磷消化率均极显著高于对照组(p<0.01);钙消化率无显著差异(p>0.05),添加本实施例对水貂氮、磷环境排放量影响显著,显著低于对照组(p<0.05);结合取皮进行水貂胃蛋白酶活性测定,采食30min后各处理间胃蛋白酶活性差异不显著(p>0.05);采食2.5h后各处理间胃蛋白酶活性差异显著(p<0.05),在采食4.5 h后各处理间差异不显著(p>0.05);添加本实施例后水貂皮长和鲜皮重差异显著(p<0.05);针毛长度和绒毛长度无显著差异(p>0.05)。 It can be seen from Table 1 that the acid value and carbonyl value of the marker fat oxidation index in this embodiment are much better than that of the control group, and the volatile base nitrogen value of the marker protein change also verifies this point. After adding this embodiment, Escherichia coli in fresh food The downward trend is obvious. It can be seen from Table 2 that after adding this example, the mink diarrhea index and diarrhea rate decreased significantly with the addition of this example ( P <0.01); there was no significant difference in dry matter intake ( p >0.05), both were around 150g, It has a significant effect on the body weight and average daily gain of minks in the growth and development period ( P <0.05); the dry matter digestibility, crude protein digestibility, nitrogen metabolism rate, and phosphorus digestibility are all significantly higher than those of the control group ( p <0.01); there was no significant difference in calcium digestibility ( p > 0.05), and the addition of this example had a significant impact on the nitrogen and phosphorus environmental emissions of mink, which was significantly lower than that of the control group ( p <0.05); the pepsin activity of mink was measured in combination with skin removal , there was no significant difference in pepsin activity among the treatments after 30 min of feeding ( p >0.05); there was a significant difference in pepsin activity among the treatments after 2.5 h of feeding ( p < 0.05), and there was no significant difference between the treatments after 4.5 h of feeding Significantly ( p >0.05); the mink fur length and fresh skin weight were significantly different after adding this example ( p <0.05); the guard hair length and villi length were not significantly different ( p > 0.05).
综上可见,本品具有很好的抗脂肪、蛋白质氧化变质功能,对水貂饲料适口性无不良影响,可提高营养物质消化利用率,降低氮、磷排放量,改善对环境的压力,对水貂生产经济效益和环境效益显著。 In summary, this product has good anti-fat and protein oxidation and deterioration functions, has no adverse effect on the palatability of mink feed, can improve the digestion and utilization of nutrients, reduce nitrogen and phosphorus emissions, and improve the pressure on the environment. The production economic benefit and environmental benefit are remarkable.
表一、用本实施例制作的添加剂对水貂饲料的各项指标的影响 Table one, the impact of the additives made by the present embodiment on the various indicators of the mink feed
表二、用本实施例制作的添加剂对水貂的各项指标的影响 Table two, the influence of the additives that present embodiment is made on the various indexes of mink
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