CN103604768A - Portable UV spectrophotometer special for CO poisoning and application method thereof - Google Patents

Abstract

The invention relates to a special portable ultraviolet spectrophotometer for CO poisoning and its use method. The device includes an integrated sampling probe, a liquid core waveguide flow cell, a pressing pump, an LED light source, a micro detector, an optical coupler, a control circuit and battery etc. The integrated sampling probe, liquid core waveguide flow cell, and optical coupler are composed of a capillary. The light source is a plug-in LED light source, and LEDs with different wavelengths can be used according to needs. The sample is introduced by pressing the pump, which is simple and reliable. The matching kit includes cleaning solution and diluent. The method of use is as follows: first clean the flow cell, further test the reference solution and the test sample solution at different detection wavelengths, and finally obtain the concentration of carboxyhemoglobin in the blood sample through software conversion, and display it on the micro detector. The beneficial effects of the invention are: simple and easy to carry, simple operation, accurate measurement of carbon monoxide concentration in blood, less sample consumption, flexible use and high popularization value.

Description

A special portable ultraviolet spectrophotometer for CO poisoning and its application method

technical field

The invention relates to a spectrophotometer, in particular to a special portable ultraviolet spectrophotometer for CO poisoning and its use method.

Background technique

With the development of science and technology and the progress of society, analysis and testing have become one of the most frequent scientific and technological activities of human beings, and the dependence of social and economic development on analysis and testing instruments has been increasing. Moreover, with the development of science, the requirements for real-time analysis and test results in various application fields are constantly increasing, and on-site rapid analysis has become one of the development trends of analytical science. On-site analysis will inevitably require the miniaturization and portability of analytical instruments, and instrument miniaturization has become an important direction for the development of analytical instruments.

Carbon monoxide poisoning incidents are easy to occur in remote areas of my country in winter. With the continuous improvement of my country's legal system, especially the increasing requirements for evidence in the criminal procedure law, blood is the only test material in carbon monoxide poisoning cases for such incidents. A measurement of the carbon monoxide concentration in the blood must be requested. When the blood is exposed to the air, the carboxyhemoglobin in the blood is easily lost by the oxygen in the air, which makes the test result inaccurate. Therefore, how to quickly and accurately measure the concentration of carbon monoxide in the blood at the place where the incident occurred, and how to promote the use at the grassroots level has become a difficulty.

At present, the determination of the degree of CO poisoning mainly depends on the determination of the carboxyhemoglobin (carbonylhemoglobin, HbCO) content [HbCO]% in the body. Specific methods include isosbestic point method [document 1. Siek T J. Determination of Carboxyhemoglobin in the Presence of Other Blood Hemoglobin Pigments by Visible Spectrophotometry [J]. Journal of Forensic Science, 1984,29: 39-54], simultaneous equations method [Document 2. Rodkey F l et al . Spectrophotometric Measurement of Carboxyhemoglobin and Methemoglobin in Blood[J]. Clinical Chemistry, 1979,25: 1388—1393], dual wavelength method [Document 3. Sakata M et al. Simple Determination of Carboxyhemoglobin by Double Wave length Spectrophotometry of Absorbance and the Comparison with Gas Chromatographic Method[J]. Forensic Science International. 1983,21: 187—195], Derivative Spectrophotometry [Document 4. Tan Jiayi, Yang Ming. Carboxyhemoglobin saturation [J]. Chinese Journal of Forensic Medicine, 1988, 3: 206-209] and so on. Among them, the derivative spectrometry requires the use of instruments capable of derivative spectrometry, and its application is limited; the isosbestic point method and the simultaneous equations method are easily affected by blood clarity, and the measurement accuracy is relatively poor; the dual-wavelength method requires two measurements , although more cumbersome, but the accuracy of the determination is higher. Ordinary UV spectrophotometers can only measure carbon monoxide in blood under laboratory conditions. The instruments are generally carried out by technicians or forensic doctors who specialize in physical and chemical testing, and the cheapest UV spectrophotometer generally costs nearly 10,000 yuan. , The operation and daily maintenance are relatively cumbersome, and it is not suitable for rapid on-site inspection. At present, the imported miniature spectrophotometer used in the market is mainly Nanodrop ND-1000, and its price is 80,000 to 90,000 yuan. It is expensive and complicated to operate, which is not conducive to popularization.     

Contents of the invention

The technical problem to be solved by the present invention is to provide a portable ultraviolet spectrophotometer for CO poisoning based on the liquid core waveguide detection principle.

A technical solution adopted by the present invention to solve its technical problems is: the special portable ultraviolet spectrophotometer for CO poisoning adopts the principle of liquid core waveguide detection, and is composed of an integrated sampling probe, liquid core waveguide flow cell, pressing pump, LED light source, micro detection device, control circuit, optocoupler and battery. The integrated sampling probe, liquid-core waveguide flow cell, and optocoupler consist of one capillary. The light source is a plug-in LED light source, and LEDs with different wavelengths can be used according to needs. The sample is introduced by pressing the pump, which is simple and reliable.

The test solution enters the liquid core waveguide flow cell from the press pump through the integrated sampling probe. The light emitted by the LED light source is changed into monochromatic light by the filter system and then enters the liquid core waveguide flow cell, and propagates along the axis in the flow cell, and finally passes through the liquid core waveguide flow cell. It is received by the detector through the optical coupler, and the test result is obtained after conversion.

This kind of ultraviolet spectrophotometer can be equipped as necessary criminal technical equipment to the criminal technical department of the public security bureau of the reclamation area (county level).

A method for using a portable ultraviolet spectrophotometer dedicated to CO poisoning, the specific steps are as follows:

1. Blood sample configuration: Take 20-50uL blood sample and dilute it 40-100 times with diluent;

2. Adjust the detection wavelength of the detector to the first wavelength of 420nm, suck the cleaning solution into the liquid-core waveguide flow cell by pressing the pump, and clean the liquid-core waveguide flow cell; then suck the reference solution into the liquid-core waveguide flow cell. Test the absorbance of the reference solution under the band, and correct the absorbance to 0;

3. After cleaning the liquid core waveguide flow cell again, suck the sample solution into the liquid core waveguide flow cell, and test its absorbance in the first band;

4. Adjust the detection wavelength of the detector to the second wavelength of 432nm, repeat steps 1 and 2, and test the absorbance of the sample solution;

5. The instrument uses a formula to calculate the concentration of HbCO in the blood sample and displays it on the screen.

The beneficial effects of the present invention are:

1. High sensitivity. The invention is based on the principle of microfluidic liquid core waveguide. The light from the light source enters the optical fiber at an optimized angle, so that the incident light propagates along the axis of the optical fiber in the optical fiber without passing through the outer wall, so that it can continuously excite the sample in the optical fiber, greatly increasing the Optical path, and improve the utilization of incident light;

2. Small size, easy to carry. Using LED as the light source, it is small in size, light in weight and high in light intensity. The integrated optical path and control system design make the whole machine small in size;

3. A small amount of sample is required. The liquid core waveguide fiber is used as the liquid core waveguide flow cell, the cell volume is less than 1μL, and the sample volume required for the entire test is very small;

4. Simple operation. By designing dedicated circuits and control programs, a series of processes such as data acquisition, signal processing and calculation, process control and result display are automated;

5. Wide range of applications. The liquid core waveguide spectrometer uses a plug-in single-color LED as the light source to measure the maximum absorption wavelength of the sample, and can also achieve multi-wavelength detection by replacing the LED light source with different wavelengths.

BRIEF DESCRIPTION OF THE DRAWINGS The present invention will be further described below in conjunction with the accompanying drawings.

Figure 1 is a schematic diagram of the present invention.

Among them: 1. LED light source, 2. Filter system, 3. Integrated sampling probe, 4. Liquid core waveguide flow cell, 5. Optical coupler, 6. Micro detector, 7. Press pump, 8. Battery, 9. Control circuit

The specific embodiment The present invention will be further described in conjunction with specific embodiment now, and following examples are intended to illustrate the present invention rather than further limiting the present invention.

Embodiment: As shown in Figure 1, a portable UV spectrophotometer for CO poisoning based on the microfluidic liquid core waveguide principle, including an integrated sampling probe (3), a liquid core waveguide flow cell (4), and a push pump (7), LED light source (1), filter system (2), optical coupler (5), miniature detector (6), control circuit (9) and battery (8), integrated sampling probe (3) , liquid core waveguide flow cell (4), optical coupler (5) are composed of a 50μm ID × 20mm Teflon AF2400 coated quartz capillary, the whole instrument is powered by battery (8) and controlled by control circuit (9), LED The light emitted by the light source (1) passes through the filter optical system (2), the liquid core waveguide flow cell (4), the optical coupler (5) in sequence, and is finally received by the micro detector (6). An effective pathlength of about 10 mm was obtained with a detection volume of 350 nL.

The Teflon AF 2400 capillary is used as the flow cell. The refractive index of the Teflon material (1.29) is lower than that of water (1.33), so the light energy is bound in the aqueous solution and advances along the inner wall of the capillary to obtain a long optical path, thereby achieving high sensitivity. purpose of consumption.

Take 20 uL of blood sample, and dilute it 40 times with diluent to prepare blood sample. Using two sets of filter systems at 420nm and 432nm, according to the previous steps, measure the absorbance A ₄₂₀ and A ₄₃₂ of the sample at two wavelengths of 420nm and 432nm, the calculation formula of carboxyhemoglobin content is [HbCO]%=(1.317A ₄₂₀ —0.988A ₄₃₂ )/(0.844A ₄₂₀ + 0.982A ₄₃₂ )×100%, the concentration of HbCO in the sample [HbCO]% is directly displayed on the instrument screen after the test.

The device has the advantages of simple structure, convenient use, low cost, wide application range, easy miniaturization and portability.

Inspired by the above-mentioned ideal embodiment according to the present invention, through the above-mentioned description content, relevant workers can make various changes and modifications within the scope of not departing from the technical idea of the present invention. The technical scope of the present invention is not limited to the content in the specification, but must be determined according to the scope of the claims.

Claims (5)

1. the poisoning special portable formula of CO uv-spectrophotometric instrument and a using method thereof, is characterized in that: device comprises LED light source (1), filter system (2), integrative sampling probe (3), liquid core waveguide flow cell (4), photo-coupler (5), micro-detector (6), Pressing pump (7), battery (8) and control circuit (9); Integrative sampling probe (3), liquid core waveguide flow cell (4), photo-coupler (5) consist of a capillary; Whole instrument is powered and is controlled by control circuit (9) by battery (8), the light that LED light source (1) emits is photosystem (2), liquid core waveguide flow cell (4), photo-coupler (5) after filtration successively, finally by micro-detector (6), received, and show on screen by conversion.

2. the poisoning special portable formula of CO according to claim 1 uv-spectrophotometric instrument and using method thereof, is characterized in that: matched reagent box is comprised of cleaning fluid and thinning agent; Cleaning fluid is 0.1-0.2%Na ₂cO ₃solution (W/W); Thinning agent is for containing 0.1-0.2% Na ₂cO ₃(W/W), 0.2-0.3%Na ₂s ₂o ₄(W/W) aqueous solution, this solution is also reference solution.

3. the poisoning special portable formula of CO according to claim 1 uv-spectrophotometric instrument and using method thereof, it is characterized in that: the light that LED light source (1) emits the first wavelength that photosystem (2) obtains after filtration is successively 420nm, and second wave length is 432nm.

4. the poisoning special portable formula of CO according to claim 1 uv-spectrophotometric instrument and using method thereof, is characterized in that: concrete method of testing is:

The configuration of A blood sample: get blood sample 20～50uL, carry out the dilution of 40～100 times with thinning agent;

B concrete operation step:

A, detecting device is detected to wavelength regulation is the first wavelength, by Pressing pump (7), cleaning fluid is sucked to liquid core waveguide flow cell (4), cleans flow cell; Then reference solution is sucked in liquid core waveguide flow cell (4), the absorbance of testing reference solution under the first wave band, proofreading and correct absorbance is 0;

B, again clean after liquid core waveguide flow cell (4), sample solution is sucked in liquid core waveguide flow cell (4), under the first wave band, test its absorbance;

C, detecting device is detected to wavelength regulation is second wave length, repeating step 1 and step 2, the absorbance of specimen solution;

D, instrument are converted to carbonyl hemoglobin concentration in blood by test result, and show on screen.

5. the poisoning special portable formula of CO according to claim 1 uv-spectrophotometric instrument and user thereof

The poisoning special portable formula of CO according to claim 1 uv-spectrophotometric instrument and using method thereof, is characterized in that: described kapillary is the Teflon kapillary of ultraviolet light thoroughly; Micro-detector (6) can be miniature photomultiplier or CCD; Filter system (2) can be optical filter, grating or monochromator.

Images