CN103585205B - The application of a kind of Chrysosplenium extract in preparation treatment diabetes medicament - Google Patents

Abstract

The invention discloses a new application of the extract of the golden waist grass, and the new use is the application of the golden waist grass extract in the preparation of medicines for treating diabetes. The golden waist extract is prepared according to the following method: golden waist grass is used as raw material, and after extraction, two macroporous adsorption resin purification techniques are used to obtain golden waist grass extract A, golden waist grass extract B, golden waist grass Herbal extract C, mixed according to a certain ratio, is the extract of Golden waist. The golden waist grass extract provided by the invention is of great significance for the preparation of medicines for treating diabetes.

Description

Application of a golden waist grass extract in the preparation of medicines for treating diabetes

technical field

The invention belongs to the field of medicine, and relates to the application of an extract of traditional Chinese medicinal materials in the preparation of medicines for treating diabetes, in particular to the application of an extract of golden waist grass in the preparation of medicines for treating diabetes.

Background technique

With the aging of the world's population, diabetes has become a common and frequently-occurring disease, and its incidence is on the rise in industrialized countries. According to statistics, there are about 150 million diabetic patients in the world, 80% of which are non-insulin-dependent diabetes (type 2), of which there are about 30 million in my country. The number of deaths caused by diabetes complications ranks third after cardiovascular and cerebrovascular diseases and cancer in developed countries, which has attracted great attention from all over the world, and researches on the pathogenesis and drug prevention and treatment of diabetes have been carried out one after another. Since the etiology of type 1 diabetes is basically clear, only insulin supplementation is enough, while type 2 diabetes is more complicated. At present, the medical field in my country adopts comprehensive treatment measures including diet therapy, drug therapy and other adjuvant therapies. Although western medicine emerges in an endless stream, it cannot fundamentally solve the problem, and western medicine treatment has certain side effects and "treatment failure". TCM treatment of diabetes has a long history, rich clinical experience, and remarkable curative effect. It has become a research hotspot at home and abroad in recent years. The understanding of diabetes in traditional Chinese medicine is different from that in western medicine. Traditional Chinese medicine emphasizes the holistic view and reflects the essence of the disease through the changes of yin and yang. The normal body is "yin and yang secret", yin and yang are balanced, that is, the internal environment of the body maintains a stable state, and the glucose metabolism also maintains stability; and the toxic and side effects of traditional Chinese medicine are relatively small, and patients can take it for a long time. This is a unique advantage that western medicine cannot match. .

Traditional Chinese medicine believes that diabetes is caused by burning yin deficiency in the lungs, stomach, and kidneys, and abnormal water and grain transfer. Its base

The pathogenesis is depletion of yin and fluid, excess of dryness and heat, deficiency of kidney yin as the root, dryness and heat of the lung and stomach as the standard, long-term illness leads to loss of yin, depletion of qi and injury of yang, resulting in damage to both qi and yin, deficiency of both yin and yang, blood stasis in the veins, The tendons and arteries are not nourished, the internal organs are damaged, and a series of concurrent diseases gradually appear.

The course of diabetes is a process of yin damage and yang. Based on yin differentiation, from mild to severe, it is the transformation of yin deficiency - deficiency of both qi and yin - deficiency of both yin and yang. And blood stasis runs through the whole process of primary disease and concurrent disease.

In recent years, some scholars have explored the relationship between TCM syndrome differentiation and clinical biochemical indicators of diabetes. From the changes of pancreatic islet function, blood lipid level, plasma sex hormone, corticosteroid level, immune function and blood rheology, etc., observe the correlation between the type of diabetes and the changes of the above indicators, and find the abnormal changes of various indicators, ranging from mild to severe , mostly develops in the direction of deficiency of yin—deficiency of both qi and yin—deficiency of both yin and yang. It embodies the material basis for exploring the classification of diabetes by combining traditional Chinese and Western medicine.

Syndrome differentiation and treatment is the essence of TCM theory. According to the main pathogenesis of diabetes, traditional Chinese medicine distinguishes the severity of dryness and heat and yin deficiency, and pays attention to nourishing yin when taking medicine. If there is dryness and heat, it should be cleared away. According to modern pharmacological concepts, the research on the anti-diabetic effect of traditional Chinese medicine is mainly as follows. Single traditional Chinese medicine: coptis and berberine, bitter gourd, ginseng, astragalus, scutellaria baicalensis, mulberry leaves, traditional Chinese medicine polysaccharides (such as tremella polysaccharides, tremella spore polysaccharides, fungus polysaccharides, Hericium erinaceus polysaccharides, laver polysaccharides, Ophiopogon japonicus polysaccharides, laminarin ), Lycium barbarum polysaccharide-X, Lycium barbarum polysaccharide-D, papaya polysaccharide and paeonol polysaccharide, etc.), silymarin, rhubarb. In addition, oral administration of flavonoids puerarin or clausenacoumarine also reduced hyperglycemia in ALX animals. The basic principles of TCM treatment of diabetes can be summarized as: nourishing yin and clearing away heat, nourishing qi and nourishing yin, nourishing kidney and spleen, promoting blood circulation and removing blood stasis, etc. Commonly used classic prescriptions include Shenbaihu Decoction, Liuwei Dihuang Pills, Bawei Dihuang Pills, and Qianjin Huanglian Pills. According to different syndrome types and the above-mentioned treatment principles, formulas can be formulated or added or subtracted from ancient prescriptions.

Golden waist grass is the dry whole plant of Chrysosplenium nudicaule Bge. and several plants of the same genus. Collect in autumn, remove dead leaves, wash and dry. It is recorded in "Ministry of Health of the People's Republic of China Drug Standard Tibetan Medicine (Volume 1)", standard number: WS3-BC-0061-95. Cold in nature, bitter in the mouth. It has the effects of clearing away heat and promoting gallbladder, and laxative. For biliary fever, fever, headache, cholecystitis, gallstones. Commonly used medicine for diarrhea. It is a commonly used drug in ethnic minority areas such as Tibet and Mongolia. However, the basic research on Golden Waist Grass is still very limited, which limits the subsequent promotion and application of this medicinal material. There are few reports on the chemical constituents and pharmacological effects of Artemisia arborii in modern research.

According to domestic patent search results, there is no patent related to golden waist grass.

In the above-mentioned literatures and patents, there is no report on the use of Phalaenopsis or Phalaenopsis extracts for the preparation of drugs for treating diabetes.

Contents of the invention

The purpose of the present invention is to provide an application of the extract of Phalaenopsis radix in the preparation of medicaments for treating diabetes.

The present invention is achieved through the following technical solutions:

The golden waist grass used in the present invention is the dry whole herb of Chrysosplenium nudicaule Bge. and several plants of the same genus.

An application of a golden waist extract in the preparation of a medicine for treating diabetes, the preparation method of the golden waist extract is:

(1) Golden waist grass, use ethanol with a concentration of 80%-100% as a solvent, and extract it by warm soaking at 20°C-70°C. The number of extractions is 2-6 times, and the extraction time is 2-8 hours each time. The amount of solvent used each time It is 10-30 times of the weight of Phyllostachys chinensis, filtered to obtain the dregs A and the extract, the extract is recovered with ethanol, concentrated, dried, and the extract A of Phalaenopsis is obtained;

(2) Use 0-50% ethanol as a solvent to extract the dregs A obtained in step (1) by warm soaking at 30°C-80°C. The number of extractions is 2-5 times, and the extraction time is 1-6 hours each time. The amount of solvent used each time is 6-20 times of the weight of Golden Waist Grass, filtered, the extract is recovered with ethanol, concentrated to relative density d=1.05-1.12, filtered to obtain liquid B;

(3) Pass the drug solution B obtained in step (2) through a non-polar macroporous adsorption resin column, and first elute with water, and the water eluent directly passes through the polar macroporous resin column, and then use a concentration of 60%-95% ethanol solution to elute the non-polar macroporous adsorption resin column, collect ethanol eluents of different concentrations, concentrate and dry, and then obtain Golden Waist Grass Extract B; Hole adsorption resin column, collect ethanol eluents with different concentrations, concentrate and dry, and then get Phalaenopsis extract C;

(4) The above-mentioned Golden Waist Grass Extract A, Golden Waist Grass Extract B, Golden Waist Grass Extract C, one or two or three of them are mixed in a certain proportion to obtain the Golden Waist Grass Extract of the present invention .

The preparation method of golden waist grass extract is:

(1) Golden waist grass, use ethanol with a concentration of 80%-100% as a solvent, and extract it by warm soaking at 20°C-70°C. The number of extractions is 2-6 times, and the extraction time is 2-8 hours each time. The amount of solvent used each time It is 10-30 times of the weight of Phyllostachys chinensis, filtered to obtain the dregs A and the extract, the extract is recovered with ethanol, concentrated, dried, and the extract A of Phalaenopsis is obtained;

(2) Use 0-50% ethanol as a solvent to extract the dregs A obtained in step (1) by warm soaking at 30°C-80°C. The number of extractions is 2-5 times, and the extraction time is 1-6 hours each time. The amount of solvent used each time is 6-20 times of the weight of Golden Waist Grass, filtered, the extract is recovered with ethanol, concentrated to relative density d=1.05-1.12, filtered to obtain liquid B;

(3) Pass the drug solution B obtained in step (2) through a non-polar macroporous adsorption resin column, and first elute with water, and the water eluent directly passes through the polar macroporous resin column, and then use a concentration of 60%-95% ethanol solution to elute the non-polar macroporous adsorption resin column, collect ethanol eluents of different concentrations, concentrate and dry, and then obtain Golden Waist Grass Extract B; Hole adsorption resin column, collect ethanol eluents with different concentrations, concentrate and dry, and then get Phalaenopsis extract C;

(4) The above-mentioned Golden Waist Grass Extract A, Golden Waist Grass Extract B, and Golden Waist Grass Extract C are mixed evenly to obtain the Golden Waist Grass Extract of the present invention.

The preparation method of preferred golden waist extract is:

(1) Golden waist grass, first use 90% ethanol as a solvent, and extract at 50°C by warm soaking. The number of extractions is 4 times, and the extraction time is 4 hours each time. The amount of solvent used each time is 20 times the weight of golden waist grass. Filtrate to obtain medicinal dregs A and extract, recover ethanol from the extract, concentrate, and dry to obtain Phalaenopsis extract A;

(2) Use 30% ethanol as a solvent to extract the dregs A obtained in step (1), warm soaking and extracting at 70°C, the number of extractions is 3 times, and the extraction time is 3 hours each time, and the amount of solvent used each time is the weight of Golden Waist Grass 15 times of that, filtered, the extract recovered ethanol, concentrated to a relative density of d=1.12, filtered to obtain medicinal solution B;

(3) Pass the drug solution B obtained in step (2) through an AB-8 non-polar macroporous adsorption resin column, and first elute with water, and the water eluate directly passes through a DM130 polar macroporous resin column, and then use a concentration of 85 % ethanol solution to elute AB-8 non-polar macroporous adsorption resin column, collect 85% concentration ethanol eluate, concentrate and dry to obtain Golden Waist Grass Extract B; then elute DM130 polarity with concentration ethanol solution Macroporous adsorption resin column, collecting 70% ethanol eluate, concentrating and drying to obtain Phalaenopsis extract C;

(4) The above-mentioned Golden Waist Grass Extract A, Golden Waist Grass Extract B, and Golden Waist Grass Extract C are mixed evenly to obtain the Golden Waist Grass Extract of the present invention.

The preparation method of the golden waist grass extract of the present invention is characterized in that: the nonpolar macroporous adsorption resin adopted is AB-8 macroporous adsorption resin, D101 macroporous adsorption resin; the adopted polar macroporous adsorption resin It is DM130 macroporous adsorption resin and LSA-10 macroporous adsorption resin.

The golden waist grass extract of the present invention and chemical medicine or traditional Chinese medicine or natural medicine form the medicine for treating diabetes.

The application of the golden waist grass extract A, the golden waist grass extract B and the golden waist grass extract C of the present invention in the preparation of medicines for treating diabetes.

The golden waist grass extract A, golden waist grass extract B, golden waist grass extract C of the present invention and chemical medicine or traditional Chinese medicine or natural medicine form the medicine for treating diabetes.

The golden waist grass extract of the present invention is prepared into pharmaceutical oral dosage forms such as tablets, granules, and capsules by adding various pharmaceutically acceptable auxiliary materials.

For the first time, the present invention has used two adsorption resin purification techniques for the preparation of the extracts for the treatment of diabetes, and obtained the extracts with the activity of treating diabetes, including the extract A, the extract B and the Waistweed Extract C.

For the first time, the present invention uses saxifrage plants Chrysosplenium nudicaule Bge. and dried whole plants of several plants of the same genus as raw materials to prepare extracts for treating diabetes. Animal experiments show that the extract of Golden Waist Grass has obvious hypoglycemic effect. Clinical observation tests have shown that the extract of Golden Waist Grass can significantly reduce urine protein and blood muscle intoxication in patients with DN, and improve renal function. The treatment group was better than the control group, but had no significant effect on fasting blood sugar (P>0.05). No obvious adverse reactions were found in the treatment group after treatment.

detailed description

The following is a further description of the application of a golden waist extract in the preparation of a drug for treating diabetes through specific experimental examples and examples, but it is not limited to the present invention.

Embodiment 1: the preparation of golden waist grass extract

(1) 16 kg of golden waist grass, use 90% ethanol as solvent, and extract by warm soaking at 50°C, the number of extractions is 4 times, each extraction time is 4 hours, and the amount of solvent used each time is 20 times the weight of golden waist grass, Filtrate to obtain medicinal dregs A and extract, recover ethanol from the extract, concentrate, and dry to obtain Phalaenopsis extract A;

(2) Use 30% ethanol as a solvent to extract the dregs A obtained in step (1), warm soaking and extracting at 70°C, the number of extractions is 3 times, and the extraction time is 3 hours each time, and the amount of solvent used each time is the weight of Golden Waist Grass 15 times of that, filtered, the extract recovered ethanol, concentrated to a relative density of d=1.12, filtered to obtain medicinal solution B;

(3) Pass the drug solution B obtained in step (2) through an AB-8 non-polar macroporous adsorption resin column, and first elute with water, and the water eluate directly passes through a DM130 polar macroporous resin column, and then use a concentration of 85 % ethanol solution to elute AB-8 non-polar macroporous adsorption resin column, collect 85% concentration ethanol eluate, concentrate and dry to obtain Golden Waist Grass Extract B; then elute DM130 with 70% ethanol solution Polar macroporous adsorption resin column, collecting 70% ethanol eluate, concentrating and drying to obtain Phalaenopsis extract C;

(4) The above-mentioned Golden Waist Grass Extract A, Golden Waist Grass Extract B, and Golden Waist Grass Extract C are mixed evenly to obtain the Golden Waist Grass Extract of the present invention.

Embodiment 2: the preparation of golden waist grass extract

(1) 24kg of Golden Waist Grass, use 80% ethanol as a solvent, and extract by warm soaking at 70°C. The number of extractions is 2 times, each extraction time is 8 hours, and the amount of solvent used each time is 30 times the weight of Golden Waist Grass. Filtrate to obtain medicinal dregs A and extract, recover ethanol from the extract, concentrate, and dry to obtain Phalaenopsis extract A;

(2) The dregs A obtained in step (1) are used as a solvent, soaked and extracted at 80°C, the number of extractions is 2 times, and the extraction time is 6 hours each time, and the amount of solvent used each time is 20 times the weight of the golden waist grass, Filter, concentrate the extract to a relative density of d=1.12, filter to obtain medicinal solution B;

(3) Pass the drug solution B obtained in step (2) through a D101 non-polar macroporous adsorption resin column, and first elute with water. % ethanol solution to elute the D101 non-polar macroporous adsorption resin column, collect the 60% concentration ethanol eluate, concentrate and dry to obtain the golden waist grass extract B; then elute LSA-10 with 85% ethanol solution Polar macroporous adsorption resin column, collect 85% concentration ethanol eluate, concentrate and dry, and obtain Phalaenopsis extract C;

(4) The above-mentioned Golden Waist Grass Extract A, Golden Waist Grass Extract B, and Golden Waist Grass Extract C are mixed evenly to obtain the Golden Waist Grass Extract of the present invention.

Embodiment 3: the preparation of golden waist grass extract

(1) Golden Waist Grass, use 100% ethanol as a solvent, extract by warm soaking at 20°C, the number of extractions is 6 times, each extraction time is 2 hours, and the amount of solvent used each time is 10 times the weight of Golden Waist Grass, filter , to obtain the dregs A and the extract, the extract recovers ethanol, concentrates, and dries to obtain the Phalaenopsis extract A;

(2) Use 50% ethanol as a solvent to extract the dregs A obtained in step (1), warm soaking and extracting at 30°C, the number of extractions is 5 times, each extraction time is 1 hour, and the amount of solvent used each time is the weight of Phalaenopsis 6 times of that, filtered, the extract recovered ethanol, concentrated to a relative density of d=1.05, filtered to obtain medicinal solution B;

(3) Pass the drug solution B obtained in step (2) through the AB-8 non-polar macroporous adsorption resin column, and first elute with water, and the water eluate directly passes through the DM130 polar macroporous resin column, and then use a concentration of 95 % ethanol solution to elute AB-8 non-polar macroporous adsorption resin column, collect 95% concentration ethanol eluate, concentrate and dry to obtain Golden Waist Grass Extract B; then elute DM130 with 60% ethanol solution Polar macroporous adsorption resin column, collect 60% concentration of ethanol eluate, concentrate and dry, and obtain Golden Waist Grass Extract C;

(4) The above-mentioned Golden Waist Grass Extract A, Golden Waist Grass Extract B, and Golden Waist Grass Extract C are mixed evenly to obtain the Golden Waist Grass Extract of the present invention.

Embodiment 4: the preparation of Golden Waist Grass Extract Tablet

Take 225g of Golden Waist Grass extract from Example 1, add 38g of starch, mix evenly, granulate, sieve, add 15g of microcrystalline cellulose, 1.5g of magnesium stearate, mix evenly, and press into 1000 tablets to obtain Golden Waist Herbal Extract Tablets.

Embodiment 5: Preparation of Golden Waist Grass Extract Granules

Take 195 g of the extract of Phyllostachys japonicus in Example 2, add 160 g of dextrin, mix evenly, granulate, dry, and sizing the granules to obtain the extract of Phalaenopsis japonicus granule.

Embodiment 6: Preparation of Golden Waist Grass Extract Capsules

Take 235 g of the extract of Phyllostachys japonicus in Example 3, add 85 g of starch, mix evenly, granulate, dry, granulate, and pack 1000 capsules to obtain the extract of Phalaenopsis japonicus.

Experimental Example 1: Clinical Observational Trial of Golden Waist Grass Extract Treating Microangiopathy in Type 2 Diabetic Nephropathy

Clinical data: A total of 40 patients were observed, including 22 males and 18 females, with an average age of 56 years. All patients had a history of diabetes for more than 10 years. All selected cases were in line with the diagnostic criteria for diabetes promulgated by WHO in 1999 and the diagnostic criteria for diabetic nephropathy in the sixth edition textbook "Internal Medicine" for colleges and universities. They were randomly divided into a treatment group and a control group of 20 cases each. There was no significant difference in gender, age, and disease course between the two groups (P>0.05), which were comparable. All patients had no acute or chronic nephritis and other kidney diseases, no urinary tract infection, fever and application of nephrotoxic drugs.

Treatment method: All patients were first given antihypertensive and hypoglycemic treatment, antihypertensive drugs used angiotensin-converting enzyme inhibitors (enalapril), hypoglycemic drugs used insulin (Ganshusen 30R), adjusted dosage according to the condition, so that fasting Blood sugar dropped below 7mmol/L, blood pressure dropped below 18.6/12.0kPa. The treatment group was additionally given tablets made from the extract of Phyllostachys japonicus in the patent example 4 of the present invention, 3 tablets/each time, 3 times a day, and 2 weeks was a course of treatment. The control group only used hypoglycemic, antihypertensive drugs and symptomatic treatment. Observe the changes of fasting blood glucose (FBG), 24-h urine protein quantification, blood-muscle compound clearance rate and endogenous muscle compound clearance rate in the two groups before and after treatment. Statistical processing: count data with X ² test, measurement data with t test.

Treatment results: the mean changes of FBG and 24h urine protein quantification, SCr and endogenous muscle clearance rate (CCr) in the two groups before and after treatment. After treatment, the 24-h urine protein quantification and blood muscle complex in the treatment group were significantly lower than those before treatment (P<0.01), and the clearance rate of endogenous muscle complex was significantly increased (P<0.01), and there was also a significant difference compared with the control group after treatment (P<0.01). <0.01). It shows that the addition of golden waist grass extract treatment can significantly reduce urine protein and blood muscle intoxication in patients with DN, and improve renal function. The treatment group was better than the control group, but had no significant effect on fasting blood sugar (P>0.05). No obvious adverse reactions were found in the treatment group after treatment.

Experimental Example 2: Hypoglycemic test of golden waist grass extract on spontaneously obese diabetic mice

Experimental animals: SPF grade, 60 spontaneously obese diabetic mice (db/db mice), 6 weeks old, weighing 38 ± 2g, were randomly divided into groups. Provided by the Animal Experiment Center of the Chinese Academy of Sciences.

Experimental drugs and instruments: Golden Waist Grass Extract, Golden Waist Grass Extract A, Golden Waist Grass Extract B, Golden Waist Grass Extract C (prepared by the method in Example 1, batch numbers are 20100303, 20100304, 20100305, 20100306 respectively) ; Positive control drugs were rosiglitazone maleate (GSK Tianjin Pharmaceutical Co., Ltd.), onetouch II blood glucose meter (Johnson & Johnson).

Grouping of animals: model control group: 10 animals, given the same amount of water; positive control group: 10 animals, given positive medicine; experimental drug group: 30 animals, divided into four groups, and given the extracts of Phyllostachys japonicus and Phyllostachys auritifolia respectively. Extract A, Golden Waist Grass Extract B, Golden Waist Grass Extract C.

Dosing: After being divided into groups, the dosing starts one week after adaptation, and the dosing lasts for 2 weeks. The dosing route is gavage. The dosage of the four extracts was converted to 400 mg/kg body weight in mice, and the dosage of rosiglitazone was 5 mg/kg body weight.

Blood glucose measurement: 1 hour after administration on the 7th and 14th day, the blood was taken from the tail vein to measure the blood glucose of the animal immediately with a onetouch II blood glucose meter. The test results are shown in Table 1.

Table 1 The effect of golden waist grass extract on the blood sugar concentration of db/db mice

Note: *p<0.05, compared with model group.

The results in Table 1 show that the extracts of Phalaenopsis have significant hypoglycemic effects on db/db mice, and the extracts of Phalaenopsis have the best hypoglycemic effect.

Claims (5)

1. an application of Herba Golden Waist Extract in the preparation of medicine for the treatment of diabetes, the preparation method of which Herba Golden Waist Extract is: (1) Golden waist grass, use ethanol with a concentration of 80%-100% as a solvent, and extract it by warm soaking at 20°C-70°C. The number of extractions is 2-6 times, and the extraction time is 2-8 hours each time. The amount of solvent used each time It is 10-30 times of the weight of Phyllostachys chinensis, filtered to obtain the dregs A and the extract, the extract is recovered with ethanol, concentrated, dried, and the extract A of Phalaenopsis is obtained; (2) Use 0-50% ethanol as a solvent to extract the dregs A obtained in step (1) by warm soaking at 30°C-80°C. The number of extractions is 2-5 times, and the extraction time is 1-6 hours each time. The amount of solvent used each time is 6-20 times of the weight of Golden Waist Grass, filtered, the extract is recovered from ethanol, concentrated to a relative density of 1.05-1.12, filtered to obtain liquid B; (3) Pass the drug solution B obtained in step (2) through a non-polar macroporous adsorption resin column, and first elute with water, and the water eluent directly passes through the polar macroporous resin column, and then use a concentration of 60%-95% ethanol solution to elute the non-polar macroporous adsorption resin column, collect ethanol eluents of different concentrations, concentrate and dry, and then obtain Golden Waist Grass Extract B; Hole adsorption resin column, collect ethanol eluents with different concentrations, concentrate and dry, and then get Phalaenopsis extract C; (4) The above-mentioned Golden Waist Grass Extract A, Golden Waist Grass Extract B, and Golden Waist Grass Extract C are mixed to obtain the Golden Waist Grass Extract. 2. application according to claim 1, is characterized in that the preparation method of described Golden Waist Herb Extract is: (1) Golden waist grass, first use 90% ethanol as a solvent, and extract at 50°C by warm soaking. The number of extractions is 4 times, and the extraction time is 4 hours each time. The amount of solvent used each time is 20 times the weight of golden waist grass. Filtrate to obtain medicinal dregs A and extract, recover ethanol from the extract, concentrate, and dry to obtain Phalaenopsis extract A; (2) Use 30% ethanol as a solvent to extract the dregs A obtained in step (1), warm soaking and extracting at 70°C, the number of extractions is 3 times, and the extraction time is 3 hours each time, and the amount of solvent used each time is the weight of Golden Waist Grass 15 times, filtered, the extract recovered ethanol, concentrated to a relative density of 1.12, filtered to obtain the medicinal solution B; (3) Pass the drug solution B obtained in step (2) through an AB-8 non-polar macroporous adsorption resin column, and first elute with water, and the water eluate directly passes through a DM130 polar macroporous resin column, and then use a concentration of 85 % ethanol solution to elute AB-8 non-polar macroporous adsorption resin column, collect 85% concentration ethanol eluate, concentrate and dry to obtain Golden Waist Grass Extract B; then elute DM130 with 70% ethanol solution Polar macroporous adsorption resin column, collecting 70% ethanol eluate, concentrating and drying to obtain Phalaenopsis extract C; (4) The above-mentioned Golden Waist Grass Extract A, Golden Waist Grass Extract B, and Golden Waist Grass Extract C are mixed to obtain the Golden Waist Grass Extract. 3. application according to claim 1, it is characterized in that the non-polar macroporous adsorbent resin that the preparation method of described Golden Waist Grass extract adopts is AB-8 macroporous adsorbent resin, D101 macroporous adsorbent resin; The polar macroporous adsorption resins used are DM130 macroporous adsorption resin and LSA-10 macroporous adsorption resin. 4. The application according to claim 1, characterized in that the drug for treating diabetes composed of the Herba Phalaenopsis extract and chemical medicine or traditional Chinese medicine. 5. The application according to claim 1, characterized in that the extract of Phyllostachys chinensis is made into pharmaceutical tablets, granules or capsules by adding various adjuvants allowed by pharmacy.