CN103583435A - Eel leucomalachite-green-removal aquaculture method - Google Patents
Eel leucomalachite-green-removal aquaculture method Download PDFInfo
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- CN103583435A CN103583435A CN201310565384.7A CN201310565384A CN103583435A CN 103583435 A CN103583435 A CN 103583435A CN 201310565384 A CN201310565384 A CN 201310565384A CN 103583435 A CN103583435 A CN 103583435A
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- 238000000034 method Methods 0.000 title claims abstract description 13
- 238000009360 aquaculture Methods 0.000 title abstract description 4
- 244000144974 aquaculture Species 0.000 title abstract description 4
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims abstract description 51
- FDZZZRQASAIRJF-UHFFFAOYSA-M malachite green Chemical compound [Cl-].C1=CC(N(C)C)=CC=C1C(C=1C=CC=CC=1)=C1C=CC(=[N+](C)C)C=C1 FDZZZRQASAIRJF-UHFFFAOYSA-M 0.000 claims abstract description 48
- 229940107698 malachite green Drugs 0.000 claims abstract description 46
- 241000251468 Actinopterygii Species 0.000 claims abstract description 15
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims abstract description 15
- 229910052760 oxygen Inorganic materials 0.000 claims abstract description 15
- 239000001301 oxygen Substances 0.000 claims abstract description 15
- 241000252073 Anguilliformes Species 0.000 claims abstract description 6
- 239000003814 drug Substances 0.000 claims abstract description 5
- 239000000693 micelle Substances 0.000 claims description 18
- 238000009395 breeding Methods 0.000 claims description 7
- 230000001488 breeding effect Effects 0.000 claims description 7
- JEGUKCSWCFPDGT-UHFFFAOYSA-N h2o hydrate Chemical compound O.O JEGUKCSWCFPDGT-UHFFFAOYSA-N 0.000 claims description 4
- 239000000203 mixture Substances 0.000 claims description 4
- 239000003643 water by type Substances 0.000 claims description 3
- 229940079593 drug Drugs 0.000 abstract 1
- 210000004027 cell Anatomy 0.000 description 6
- HFACYLZERDEVSX-UHFFFAOYSA-N benzidine Chemical compound C1=CC(N)=CC=C1C1=CC=C(N)C=C1 HFACYLZERDEVSX-UHFFFAOYSA-N 0.000 description 4
- 238000006555 catalytic reaction Methods 0.000 description 3
- 238000001514 detection method Methods 0.000 description 3
- 238000012360 testing method Methods 0.000 description 3
- TVHAMVOINIHMEX-UHFFFAOYSA-N 3-amino-5-morpholinomethyl-2-oxazolidinone Chemical compound O1C(=O)N(N)CC1CN1CCOCC1 TVHAMVOINIHMEX-UHFFFAOYSA-N 0.000 description 2
- 102000004310 Ion Channels Human genes 0.000 description 2
- 230000000711 cancerogenic effect Effects 0.000 description 2
- 239000003183 carcinogenic agent Substances 0.000 description 2
- 210000000170 cell membrane Anatomy 0.000 description 2
- 230000017858 demethylation Effects 0.000 description 2
- 238000010520 demethylation reaction Methods 0.000 description 2
- 238000005516 engineering process Methods 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 210000001082 somatic cell Anatomy 0.000 description 2
- 241000907663 Siproeta stelenes Species 0.000 description 1
- 208000031320 Teratogenesis Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000003115 biocidal effect Effects 0.000 description 1
- 231100000357 carcinogen Toxicity 0.000 description 1
- 231100000315 carcinogenic Toxicity 0.000 description 1
- 238000006243 chemical reaction Methods 0.000 description 1
- WIIZWVCIJKGZOK-RKDXNWHRSA-N chloramphenicol Chemical compound ClC(Cl)C(=O)N[C@H](CO)[C@H](O)C1=CC=C([N+]([O-])=O)C=C1 WIIZWVCIJKGZOK-RKDXNWHRSA-N 0.000 description 1
- 229960005091 chloramphenicol Drugs 0.000 description 1
- 230000000857 drug effect Effects 0.000 description 1
- 230000029142 excretion Effects 0.000 description 1
- PLHJDBGFXBMTGZ-WEVVVXLNSA-N furazolidone Chemical class O1C([N+](=O)[O-])=CC=C1\C=N\N1C(=O)OCC1 PLHJDBGFXBMTGZ-WEVVVXLNSA-N 0.000 description 1
- 230000002068 genetic effect Effects 0.000 description 1
- 238000011835 investigation Methods 0.000 description 1
- 230000003340 mental effect Effects 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 231100000219 mutagenic Toxicity 0.000 description 1
- 230000003505 mutagenic effect Effects 0.000 description 1
- IAIWVQXQOWNYOU-FPYGCLRLSA-N nitrofural Chemical class NC(=O)N\N=C\C1=CC=C([N+]([O-])=O)O1 IAIWVQXQOWNYOU-FPYGCLRLSA-N 0.000 description 1
- NXFQHRVNIOXGAQ-YCRREMRBSA-N nitrofurantoin Chemical class O1C([N+](=O)[O-])=CC=C1\C=N\N1C(=O)NC(=O)C1 NXFQHRVNIOXGAQ-YCRREMRBSA-N 0.000 description 1
- 238000011017 operating method Methods 0.000 description 1
- 150000002894 organic compounds Chemical class 0.000 description 1
- 244000045947 parasite Species 0.000 description 1
- 238000013518 transcription Methods 0.000 description 1
- 230000035897 transcription Effects 0.000 description 1
- 239000000273 veterinary drug Substances 0.000 description 1
Classifications
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/80—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in fisheries management
- Y02A40/81—Aquaculture, e.g. of fish
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- Farming Of Fish And Shellfish (AREA)
Abstract
An eel leucomalachite-green-removal aquaculture method comprises the following steps that the diameter of an ecological fishpond is 2-10 meters, the depth of water is 1.5 meters, the volume of the water is 4.7-25 cubic meters, 250-1250 kilograms of fishes are placed in the fishpond, and the fishes are bred in the environment at the temperature of 20-30 DEG C; the fishes are bred for 10 days, no fodder or drug is fed, eels lose weight by 10% after 10 days, and 225-1125 kilograms of the fresh and alive eels are obtained; micro-cluster water and air are mixed and fed into the fishpond; the micro-cluster water and the air are fed for 0.5 hour, in addition, ordinary water is fed slowly for 1.5 hours, and the water in the fishpond flows slowly; operation is conducted in this way alternately, and 24-hour automatic operation is conducted; the fishes are bred for 8-12 days. According to the eel leucomalachite-green-removal aquaculture method, the micro-cluster water with the high content of reactive oxygen enters cells, eucomalachite green is oxidized into malachite green, the malachite green is brought out of the cells and discharged outside bodies, and the eels reach an export standard.
Description
Technical field
The present invention relates to a kind of cultural method, particularly eel procrypsis malachite green is got rid of cultural method.
Background technology
Malachite green is a kind of artificial synthetic organic compound.Because the color similarity of its color and natural malachite is gained the name.
Since within 1933, finding that malachite green has the drug effects such as antibiotic, desinsection, many countries are extensively in order to killing water product parasite, mould etc.
In fish somatic cell, under reductase catalysis, the procrypsis malachite green that malachite green is reduced in procrypsis malachite green and eel body cannot be got rid of, and is the lifelong residue of eel.
The easy demethylation of procrypsis malachite green forms the structure similar in appearance to benzidine, and benzidine is the first carcinogenic substance, and the base that its structure is easy to replace in DNA is matched, and causes DNA genetic transcription sudden change.Here it is, and that malachite green has is carcinogenic, teratogenesis, mutagenic reason, and the mankind have eaten with the aquatic products of procrypsis malachite green very harmful.
On July 7th, 2005, the national Ministry of Agriculture issued < < about organizing the emergency notice > > of the forbidding veterinary drugs such as investigation " malachite green " to check illegal business in China severely, used the behavior of " malachite green ".But owing to also there is no at a low price and effectively substitute.Malachite green aquaculture, transport with the process of peddling in remain incessant after repeated prohibition.
Summary of the invention
Technical solution of the present invention, in fish somatic cell, 80% is water, take water as medium.Malachite green positively charged, for water-soluble, is easily brought in cell by water.But in cell, under the catalysis of reductase, be converted into the colourless malachite green of uncharged neutrality, or claim procrypsis malachite green.And procrypsis malachite green is no longer water-soluble, therefore cannot be taken out of extracellular by water, cannot remove.As removed, have only procrypsis malachite green is oxidized to malachite green.Under the association parcel of water, could discharge extracellular, and release external.
Malachite green is reduced to procrypsis malachite green under reductase catalysis:
The easy demethylation of procrypsis malachite green forms the structure similar in appearance to benzidine:
As everyone knows, benzidine is the first carcinogen, and its structure is as follows:
Ion channel diameter because of cell membrane is only 2 nanometers again, only has small-micelle water (0.5---0.8 nanometer) could pass through ion channel in cell membrane.
Meanwhile, small-micelle water enters in cell with active oxygen, just procrypsis malachite green can be oxidized back to malachite green, and discharge extracellular, excretes.
Eel procrypsis malachite green is got rid of cultural method, comprises the steps:
1) Ecological Fish Culture pond diameter is 2-10 meters, 1.5 meters of the depth of waters, and water is 4.7-25 cubic metres, puts 500-2500 jin of fishes, under the environment of 20-30 degrees Celsius, puts in a suitable place to breed;
2) time of breeding fish is 10 days, do not throw something and feed any feed and medicine, eel weight reducing 10%, i.e. 450-2250 jin of fresh and alive eels after 10 days;
3) small-micelle water is mixed and passed in pond with air;
4) setting the water flowing aerating time is 0.5 hour, within other 1.5 hours, slowly passes into light water, keeps the water in pond slowly to flow;
5) water flowing aerating and slowly water flowing, hocket according to this, running automatically in 24 hours, and the time of breeding fish continues 8-12 days again.
As a further improvement on the present invention: the small-micelle water passing into is 0.5-0.8 nanometer.
As a further improvement on the present invention: small-micelle water enters in cell with active oxygen, procrypsis malachite green being oxidized back to malachite green gets rid of external.
As a further improvement on the present invention: small-micelle water water inlet pipe and oxygen increasing tube mutually also, mix water oxygen and enter in ecological culture pond together.
Compared with prior art, the invention has the beneficial effects as follows: adopt the high small-micelle water containing active oxygen to enter in cell, procrypsis malachite green is oxidized to malachite green, take extracellular out of, excrete, make eel reach export standard.
embodiment
Eel procrypsis malachite green is got rid of cultural method, comprises the steps:
1) Ecological Fish Culture pond diameter is 2-10 meters, 1.5 meters of the depth of waters, and water is 4.7-25 cubic metres, puts 500-2500 jin of fishes, under the environment of 20-30 degrees Celsius, puts in a suitable place to breed;
2) time of breeding fish is 10 days, do not throw something and feed any feed and medicine, eel weight reducing 10%, i.e. 450-2250 jin of fresh and alive eels after 10 days;
3) small-micelle water is mixed and passed in pond with air;
4) setting the water flowing aerating time is 0.5 hour, within other 1.5 hours, slowly passes into light water, keeps the water in pond slowly to flow;
5) water flowing aerating and slowly water flowing, hocket according to this, running automatically in 24 hours, and the time of breeding fish continues 8-12 days again.
The small-micelle water passing into is 0.5-0.8 nanometer.
Small-micelle water enters in cell with active oxygen, procrypsis malachite green is oxidized back to malachite green and gets rid of external.
Small-micelle water water inlet pipe and oxygen increasing tube mutually also, mix water oxygen and enter in ecological culture pond together.
operating procedure is as follows:
the present invention adopts mature technology that water is become to small-micelle water.Small-micelle water water inlet pipe and oxygen increasing tube mutually also, mix water oxygen and enter in ecological culture pond together.Allow eel move, accelerate metabolism, accelerate excretion.
Once in another monograph (patent No. is 201320189322X) " a kind of original ecological breeding fishpond cylinder assembly ", with common clear water cultivation eel, after 10 days, tested, result is as follows:
| Inspection item | Testing result | Unit | Detection method |
| Malachite green | <0.5* | ug/kg | GB/T 19857-2005 |
| Procrypsis malachite green | 8.4 | ug/kg | GB/T 19857-2005 |
| Cistofuran metabolite | <0.5* | ug/kg | FIC B074-2004 |
| Furacilin metabolite AHD | <0.5* | ug/kg | FIC B074-2004 |
| AMOZ AMOZ | <0.5* | ug/kg | FIC B074-2004 |
| Furaxone metabolite AOZ | <0.5* | ug/kg | FIC B074-2004 |
| Chloramphenicol | <0.1* | ug/kg | GB/T 20756-2006 |
* represent to detect lower bound
Result shows: other medicine and malachite Green City are got rid of, but procrypsis malachite green is still high-load, is difficult to remove.
Being placed in same ecological pool with a collection of eel, with the oxygen containing small-micelle water of height, cultivate again 10 days, send same testing agency to carry out the special detection of procrypsis malachite green, its result is as follows:
| Inspection item | Testing result | Unit | Detection method |
| Malachite green | <0.5* | ug/kg | GB/T 19857-2005 |
| Procrypsis malachite green | <0.5* | ug/kg | GB/T 19857-2005 |
* represent to detect lower bound
Result shows, procrypsis malachite green is got rid of, and eel reaches export standard.
In sum, those of ordinary skill in the art reads after file of the present invention, according to technical scheme of the present invention and technical conceive, without creative mental labour, makes other various corresponding conversion scheme, all belongs to the scope that the present invention protects.
Claims (4)
1. eel procrypsis malachite green is got rid of cultural method, it is characterized in that comprising the steps:
2-10 meters of Ecological Fish Culture pond diameters, 1.5 meters of the depth of waters, water is 4.7-25 cubic metres, puts 500-2500 jin of fishes, under the environment of 20-30 degrees Celsius, puts in a suitable place to breed;
The time of breeding fish is 10 days, do not throw something and feed any feed and medicine, eel weight reducing 10%, i.e. 450-2250 jin of fresh and alive eels after 10 days;
Small-micelle water is mixed and passed in pond with air;
Setting the water flowing aerating time is 0.5 hour, within other 1.5 hours, slowly passes into light water, keeps the water in pond slowly to flow;
5) water flowing aerating and slowly water flowing, hocket according to this, running automatically in 24 hours, and the time of breeding fish continues 8-12 days again.
2. eel procrypsis malachite green according to claim 1 is got rid of cultural method, it is characterized in that: the small-micelle water passing into is 0.5-0.8 nanometer.
3. eel procrypsis malachite green according to claim 1 is got rid of cultural method, it is characterized in that: small-micelle water enters in cell with active oxygen, procrypsis malachite green is oxidized back to malachite green and gets rid of external.
4. eel procrypsis malachite green according to claim 1 is got rid of cultural method, it is characterized in that: small-micelle water water inlet pipe and oxygen increasing tube phase also, mix water oxygen and enter in ecological culture pond together.
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| CN201310565384.7A CN103583435B (en) | 2013-11-13 | 2013-11-13 | Eel leucomalachite-green-removal aquaculture method |
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| CN103583435B CN103583435B (en) | 2015-07-15 |
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Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104012461A (en) * | 2014-06-23 | 2014-09-03 | 上海申航热能科技有限公司 | Culture pond utilizing small molecular group water circulation |
| CN104012460A (en) * | 2014-06-22 | 2014-09-03 | 上海申航热能科技有限公司 | High-density cultivation system using microcluster water and aeration |
| CN105746419A (en) * | 2016-04-27 | 2016-07-13 | 广东黄沙渔业基地有限公司 | Slimming fish pond culture device and culture method thereof |
| CN106106283A (en) * | 2016-06-30 | 2016-11-16 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of compartment application ozone improves the method for fish slimming effect |
| CN106172091A (en) * | 2016-06-30 | 2016-12-07 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of compartment application chlorine dioxide improves the method for fish slimming effect |
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| CN103353495A (en) * | 2011-11-15 | 2013-10-16 | 南昌大学 | Method for enriching leucomalachite green and leucogentian violet in aquatic products |
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2013
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| US5397466A (en) * | 1993-06-18 | 1995-03-14 | Mytrex Industries, Inc. | Circulating filter and aerator system for use in aquaculture |
| CN1256069A (en) * | 1998-12-09 | 2000-06-14 | 中国科学院南京地理与湖泊研究所 | Artificial breeding technology for dark-figured oriental porpoise |
| CN1425281A (en) * | 2001-12-14 | 2003-06-25 | 辽宁省淡水水产研究所 | Large-scale artificial breeding technology for yellow cartfish |
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Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104012460A (en) * | 2014-06-22 | 2014-09-03 | 上海申航热能科技有限公司 | High-density cultivation system using microcluster water and aeration |
| CN104012461A (en) * | 2014-06-23 | 2014-09-03 | 上海申航热能科技有限公司 | Culture pond utilizing small molecular group water circulation |
| CN105746419A (en) * | 2016-04-27 | 2016-07-13 | 广东黄沙渔业基地有限公司 | Slimming fish pond culture device and culture method thereof |
| CN106106283A (en) * | 2016-06-30 | 2016-11-16 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of compartment application ozone improves the method for fish slimming effect |
| CN106172091A (en) * | 2016-06-30 | 2016-12-07 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of compartment application chlorine dioxide improves the method for fish slimming effect |
| CN106106283B (en) * | 2016-06-30 | 2019-04-05 | 广东省农业科学院蚕业与农产品加工研究所 | A kind of method that compartment application ozone improves fish slimming effect |
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| CN103583435B (en) | 2015-07-15 |
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