CN103583360B - A kind of directional induction improves the method for Abelia biflora nursery stock salt resistance - Google Patents

Abstract

The invention discloses a method for directional induction to improve the salt tolerance of hexagram seedlings, comprising the following steps: (1) sterilization of materials, (2) induction of adventitious buds, (3) differentiation and subculture of buds, (4) ) rooting culture, (5) seedling hardening, (6) transplanting, (7) seedling management. The present invention utilizes the means of tissue culture and container seedling cultivation to gradually add different concentrations of NaCl to the differentiation and value-added medium, the rooting medium, and the substrate for seedling cultivation after transplanting seedlings, so as to increase the salt tolerance of the seedlings from about 0.2% to 0.6%. About (0.4-0.5% NaCl content in the substrate for one-year seedlings can grow normally, and 0.5-0.6% NaCl content in the substrate for biennial seedlings can grow normally), and the survival rate of colonization in saline-alkali land has more than doubled.

Description

A method of directional induction to improve the salt tolerance of hexagram seedlings

technical field

The invention relates to the technical field of garden tree cultivation, and specifically refers to a method for obtaining hexagram seedlings and improving salt and alkali resistance by using modern seedling raising techniques.

Background technique

It is native to my country, and it is a deciduous shrub of the Lonicera family, and it is the most famous ornamental tree species with colorful leaves in my country. Rodaoki has strong adaptability, low temperature resistance, resistance to poor soil, and has a certain resistance to salt and alkali and drought. Liudaomu is one of the important landscaping tree species in the southeast coast of my country. However, the southeast coast is located in the subtropical zone and has a large annual rainfall. The salinized soil is gradually desalinated by rainwater and salt. The salt composition is mainly chloride, with a salt content of 0.1 %-0.8%.

Contents of the invention

Although the hexagram has a certain salt tolerance, years of research by the inventor's research team have confirmed that the growth of the hexagram is hindered when the soil salinity is above 0.2%, and 0.3% is the threshold for its growth. The propagation of Hexa chinensis generally adopts methods such as cutting, seeding and tissue culture. Tests have shown that if cutting and seeding propagation are adopted, the salt content gradually increased in the seedling medium can also improve the salt tolerance of seedlings, but through tissue culture and As a means of rapid propagation, the salt content is gradually increased from the substrate of tissue culture to the substrate of seedlings, and the salt tolerance of the produced seedlings is higher than that of seedlings obtained by cutting and seedling propagation. The invention is based on the theory of plant domestication step by step, from the differentiation stage of tissue culture buds to the nursery stock stage, and improves the salt tolerance of the hexapodactylus through manual intervention and domestication.

The technical problem to be solved by the present invention is to provide a method for directional induction to improve the salt tolerance of hexagram seedlings in view of the high salt content and low survival rate of hexagram seedlings planted in the tidal flats and beaches in the southeast coast of my country. The present invention utilizes the means of tissue culture and container seedling cultivation to gradually add different concentrations of NaCl to the differentiation and value-added medium, the rooting medium, and the substrate for seedling cultivation after transplanting seedlings, so as to increase the salt tolerance of the seedlings from about 0.2% to 0.6%. About (NaCl content 0.4-0.5% in the one-year seedling substrate can grow normally, and the NaCl content 0.5-0.6% can grow normally in the biennial seedling substrate), and the survival rate of colonization in saline-alkali land is more than doubled.

The technical solution adopted by the present invention to solve the above-mentioned technical problems is: the method includes the induction of the hexagram buds, the differentiation and proliferation cultivation, the induction of the root system and the cultivation of the seedlings, etc.,

(1) Sterilization of materials: Take the young branches of Liudaomu, cut them into 4-5cm stems with axillary buds, soak them in the washing liquid for 5-6 minutes, then rinse them with water for 2-3 hours, then cut off the unfolded leaves. into a 1.5cm stem segment with axillary buds; soak it in a 75% alcohol solution for 20 seconds on a clean bench, then sterilize it in a 0.1% mercuric chloride solution for 8-10 minutes, and then rinse it with sterile water 5-8 times;

(2) Induction of adventitious buds: the stem segment sterilized by the step (1) is connected to No. 1 medium and cultivated for 40-50 days;

Wherein, the No. 1 medium is added with a mass fraction of 0.1% NaCl on the basis of MS medium;

(3) Differentiation and subculture of buds: select well-differentiated stem segments of buds to be connected to No. 2 medium, select well-grown buds to transfer to the same No. 2 medium for subculture once, and then transfer Cultivate 2 generations on No. 3 medium; after several generations of culture, clustered buds appear;

Wherein, the No. 2 medium is based on the MS medium with a mass fraction of 0.1% NaCl; the No. 3 medium is based on the MS medium with a mass fraction of 0.2% NaCl;

(4) rooting culture: select well-grown cluster buds to be divided into individual plants, and transfer to No. 4 medium to induce rooting; cultivate for 15-20 days, with more than 5 root systems, and transplant seedlings with a seedling height of 4-6cm; the No. 4 culture Add 0.3% NaCl on the basis of 1/2MS medium;

(5) Seedling hardening: Open the bottle mouth of the well-grown rooted seedlings, open the bottle and harden the seedlings at room temperature for 2-3 days, take out the test-tube seedlings, rinse off the root culture medium with clear water, and transplant to a place where sea sand is provided. Carry out seedling hardening on the sand bed; the salt content of the sea sand is 0.4%; the distance between rows and plants during hardening is 10×10cm; the new roots grow out and are transplanted into the container after the new leaves are taken out for cultivation; during the cultivation, the sea sand The salt content was kept constant at 0.4%;

(6) Transplanting seedlings: 1-year-old Liudaomu container substrate proportioning is peat according to the volume ratio: sea sand=7:3, slow-release compound fertilizer 1kg m ⁻³ ; 2-year-old Liudaomu container matrix proportioning is peat according to the volume ratio : sea sand=6:4, slow-release compound fertilizer 2kg.m ⁻³ ; add NaCl when preparing the substrate, the NaCl content in the one-year seedling substrate is 0.5%, and the NaCl content in the biennial seedling substrate is 0.6%;

(7) Management at the seedling stage: the container seedlings are placed in multi-span greenhouses, the temperature in the multi-span greenhouses is maintained at 20-25° C., and the relative humidity of the soil is maintained at 65-75%.

In the step (2), the No. 1 medium is further added with the following substances: 0.8-1.0 mg/L kinetin, 0.08-0.12 mg/L indolebutyric acid, and 3% sucrose.

In the step (3), the No. 2 medium contains the following substances: kinetin at 0.4-0.6 mg/L, 6-benzylaminoadenine at 0.08-0.12 mg/L, naphthalene at 0.05 mg/L Acetic acid, and sucrose with a mass fraction of 3%. Among them, mg/L represents the number of milligrams of substances contained in each liter of medium.

In the step (3), the No. 3 medium also adds the following substances: kinetin at 0.4-0.6 mg/L, 6-benzylaminoadenine at 0.08-0.12 mg/L, 0.04-0.06 mg/L Naphthalene acetic acid, and sucrose with a mass fraction of 3%.

In the step (4), the No. 4 medium is further added with the following substances: 0.4-0.6 mg/L indole butyric acid, and 2% sucrose by mass fraction.

The culture conditions of the step (2), step (3) and step (4) are all 24-25°C, relative air humidity 70-75%, light intensity 2500-3000Lx, light 10 hours/day.

In the step (5), use a sunshade net with a light transmittance of 50% within 10 days after transplanting the seedlings.

In described step (5), described sand bed comprises bottom layer, middle layer and upper layer from bottom to top; Described bottom layer is the pebble of 8-15cm, and described middle layer is the soil of 5cm, and described upper layer is seawater of 20cm. sand.

In the step (6), the 1-year seedling container uses a mesh bag container with a diameter of 4-5cm and a height of 8-10cm; the 2-year seedling container uses a mesh bag container or a plastic container with a diameter of 18-20cm and a height of 300-10cm. 310cm.

In described step (7), change basin after the 2nd year according to the growth state of seedling, select mesh bag container or plastic container, diameter is 18-20cm, and height is 300-310cm, pot soil amount 800-1000g.

In described step (7), utilize the spray facility of multi-span greenhouse to carry out sprinkling irrigation.

In the described step (7), the container seedlings are shaded during the high temperature period in summer, and the light transmittance of the shade is 60% of the total illumination.

During said step (5), step (6) and step (7), the air temperature is greater than or equal to 18°C, and the soil matrix temperature is not lower than 15°C.

Glossary

Washing liquid: refers to the washing powder aqueous solution with a mass percentage concentration of washing powder of 0.4% to 1%. The washing powder can be a common brand of washing powder that can be purchased on the market. The washing powder can also be made of dodecane Sodium phenyl sulfonate instead.

MS medium: the components are shown in Table 1

Table 1: Basic composition list of MS medium

1/2 MS medium: It means that on the basis of the above-mentioned MS medium, the concentration of macroelements is halved, and the rest of the components remain unchanged.

Salt content: The "salt content" mentioned in this application refers to the mass percentage concentration of NaCl.

Content: The "content" mentioned in this application refers to the mass percentage concentration.

Mesh bag container: refers to a container made of degradable fiber mesh material.

Plastic container: refers to a container made of plastic material.

Detachable plastic container: it is a common cultivation container in the soilless cultivation technology of seedlings, which can be obtained through commercial purchase. Its surroundings are honeycomb-shaped, and the two ends of the container are fixed with slots when raising seedlings, and the container is in the shape of a cave, and the slots are removed during planting, and the container is in a flat shape.

Pot volume: The weight of substrate contained in a container.

Advantages and beneficial effects of the present invention:

1. The present invention successfully adopts the means of tissue culture and container seedling cultivation, gradually increases the salt content in the substrate to carry out salt tolerance domestication, and the salt tolerance of the obtained seedlings is more than 60% higher than that of conventional seedling cultivation. According to the invention, the NaCl content of 0.4%-0.5% in the matrix of the annual seedlings of the hexagram can grow normally, and the NaCl content of 0.5%-0.6% in the matrix of the biennial seedlings can grow normally.

2. The present invention gradually increases the salt content in the matrix by determining the matrix formula for induction, differentiation, subculture and rooting culture of Helicia chinensis buds, hardening of seedlings, and transplantation into containers, so as to obtain a complete set of methods for improving the salt-alkali tolerance of Heliconia chinensis seedlings method. The key of the invention lies in the configuration of the culture medium at each stage, the control of the salt content in the culture medium and the screening of plants at each stage.

3. Although the present invention obtains the high seedling stock of salt tolerance by the means of step by step domestication, it does not mean that the seedling stock obtained by utilizing the seedling stock as the female parent to carry out the conventional propagation (such as cutting, grafting and seed propagation) of the next generation has High salt tolerance.

detailed description

The present invention is described in further detail below in conjunction with the examples, but the present invention is not limited only to the following examples.

Embodiment 1 Improving Roxodoni "Edward" "(Abelia 'EdwardGoucher') is the method for salt tolerance

1. Disinfection of materials: Take the young branches of Liudaomu "Edward", cut them into 4-5cm stems with axillary buds, and first wash them in washing liquid with washing powder (washing liquid refers to the content of dodecylbenzene with a mass fraction of about 0.5%) sodium sulfonate aqueous solution) for 5-6 minutes, then rinsed with tap water for 2-3 hours, then cut off the unfolded leaves, and cut them into about 1.5cm bud stem segments. On the ultra-clean workbench, soak in 75% alcohol solution for 20 seconds, then transfer to 0.1% mercuric chloride solution for sterilization for 8-10 minutes, and rinse with sterile water for 5-8 times.

2. Induction of adventitious buds: the sterilized stem segments were cultured on the medium of MS + kinetin 1.0 mg/L + indolebutyric acid 0.1 mg/L + 3% sucrose + 0.1% NaCl 40-50 days. During the bud induction process, the stem segments with good bud differentiation and growth were continuously screened.

3. Differentiation and subculture of buds: select well-differentiated stem segments and receive MS+6-benzylaminoadenine 0.1 mg/L+ kinetin 0.5 mg/L+ naphthalene acetic acid 0.05 mg/L+ mass fraction of 3% sucrose + mass fraction is 0.1% on the proliferation medium of NaCl, select well-growing buds to transfer the same medium (MS+6-benzylaminoadenine 0.1mg/L+kinetin 0.5mg/L+naphthaleneacetic acid 0.05mg/L+mass The fraction is 3% sucrose+mass fraction is 0.1% NaCl), then transferred to MS+6-benzylaminoadenine 0.1mg/L+kinetin 0.5mg/L+naphthaleneacetic acid 0.05mg/L+mass Cultured on medium with 3% sucrose + 0.2% NaCl for 2 generations. Clustered shoots appear after several generations of cultivation.

4. Rooting culture: Select well-growing clustered shoots and divide them into individual plants, and transfer them to 1/2MS+indolebutyric acid 0.5mg/L+mass fraction of 2% sucrose+mass fraction of 0.3% NaCl rooting medium to induce rooting. Cultivate for 15-20 days, the root system is about 5 or more, and the seedling height is 4-6cm.

The above culture conditions in the tissue culture room are about 25°C, about 70% relative air humidity, about 2500Lx light intensity, and 10 hours of light per day.

5. Seedling hardening: Eliminate poorly growing tissue cultured seedlings, open the bottle of well-grown rooted seedlings, open the bottle at room temperature for 2-3 days, take out the test-tube seedlings, rinse off the root culture medium with clean water, Transplant to sand bed for seedling hardening. The bottom layer of the sand bed is about 10cm of pebbles, the middle is about 5cm of soil, and the upper layer is about 20cm of sea sand (the salt content of sea sand is about 0.4%). When hardening the seedlings, the row-to-plant spacing is 10×10 cm, and automatic sprinkler irrigation is performed irregularly according to the humidity of the soil and seedling leaves to keep the leaves of the seedlings moist without losing water. After the new roots grow, the humidity of the sand bed is gradually reduced, and after the new leaves are drawn out, they are transplanted into containers for cultivation. During the period, pay attention to the salt content in the substrate is basically kept constant, and when the salt content in the substrate decreases, add an appropriate amount of NaCl to the sprinkler water.

6. Transplanting seedlings: Use sunshade nets (about 50% light transmittance) within about 10 days after transplanting seedlings. The 1-year growth seedling container mesh bag container has a diameter of 4-5cm and a height of 8-10cm; the 2-year growth seedling container mesh bag container and detachable plastic container have a diameter of 18-20cm and a height of 300-310cm. Generally, the test matrix of Liudaomu 1-year-old container is peat: sea sand = 7:3, slow-release compound fertilizer (1kg m-3) according to the volume ratio; the matrix of the 2-year-old container is peat according to the volume ratio: sea sand = 6:4, slow-release compound fertilizer (2kg.m-3). In order to prevent seedlings from being damaged by diseases and insect pests, the matrix must be strictly sterilized, and NaCl is added when preparing the matrix. The NaCl content in the matrix for annual seedlings is about 0.4%, and the NaCl content in the matrix for biennial seedlings is about 0.5%.

7. Seedling stage management: Container seedlings are placed in multi-span greenhouses equipped with sunshade and spraying facilities. The temperature in the greenhouse is kept at 20-25°C, and the relative humidity of the soil is kept at 65-75%. Change pots after the 2nd year according to the growth status of seedlings, and when the biennial seedling matrix changes pots, the matrix is sea sand=6:4 according to the volume ratio, slow-release compound fertilizer (2kg.m-3); The NaCl content is 0.5%. The container used can be a mesh bag container or a detachable plastic container, with a diameter of 18-20cm, a height of 300-310cm, and a potting soil volume of 800-1000g. For container seedlings with missing plants or poor growth, the seedlings should be replenished in time. Water immediately after filling the seedlings. It is better to use sprinkler irrigation in large-scale production, and multi-span greenhouse spray facilities can be used for sprinkler irrigation. Irrigate according to the temperature and humidity changes in the weather and seasons. Watering should be done thoroughly. The best time for watering is morning or evening.

During the above seedling hardening, transplanting and seedling raising, the air temperature should not be lower than 18°C, and the soil (substrate) temperature should not be lower than 15°C.

The slow-release compound fertilizer has been applied to the substrate, and generally there is no need to apply fertilizer again, but it can be combined with watering according to the growth status of the container seedlings and appropriate topdressing outside the root. During the high temperature period in summer, the container seedlings need to be shaded, and the light transmittance of the shade is about 60% of the full sunlight. If any disease occurs, remove the diseased plants in time, and use corresponding pesticides to spray and sterilize. If pests occur, control them in time. At the seedling stage, it is found that the substrate in the container sinks, and the substrate must be added in time to prevent the roots from being exposed. In seedling management, attention should also be paid to keeping the salt content in the substrate basically constant. When the salt content in the substrate decreases, add an appropriate amount of NaCl to the irrigation water.

Practice has proved that the growth of Rodochia "Edward" is hindered when the soil salt content is above 0.2%, and 0.3% is the threshold for its growth. After being treated by the method of Example 1, the annual seedlings of Rodaogi "Edward" with a NaCl content of 0.4% in the substrate can grow normally, and the biennial seedlings with a NaCl content of 0.5% in the substrate can grow normally.

Embodiment 2 improves the method for the salt tolerance of Abelia grandiflora (formal name used at school: Abelia grandiflora " Francis Mason ")

1. Disinfection of materials: Take the young branches of Hexaphyllum mosaicus, cut them into 4-5cm stems with axillary buds, and first wash them in washing liquid with washing powder (washing liquid refers to dodecylbenzenesulfonium containing 0.5% by mass fraction) Aqueous solution of sodium bicarbonate) for 5-6 minutes, then rinsed with tap water for 3 hours, then cut off the unfolded leaves, and cut into 1.5cm bud stem segments. On the ultra-clean workbench, soak in 75% alcohol solution for 20 seconds, then transfer to 0.1% mercuric chloride solution for sterilization for 8-10 minutes, and rinse with sterile water for 6-8 times.

2. Induction of adventitious buds: the sterilized stem segments were cultured on the medium of MS + kinetin 1.0 mg/L + indolebutyric acid 0.1 mg/L + 3% sucrose + 0.1% NaCl 40-50 days. During the bud induction process, the stem segments with good bud differentiation and growth were continuously screened.

3. Differentiation and subculture of buds: select well-differentiated stem segments and receive MS+6-benzylaminoadenine 0.1 mg/L+ kinetin 0.5 mg/L+ naphthalene acetic acid 0.05 mg/L+ mass fraction of 3% sucrose + mass fraction is 0.1% on the proliferation medium of NaCl, select well-growing buds to transfer the same medium (MS+6-benzylaminoadenine 0.1mg/L+kinetin 0.5mg/L+naphthaleneacetic acid 0.05mg/L+mass 3% sucrose+mass fraction of 0.1% NaCl (proliferation medium) was subcultured once, and then transferred to MS+6-benzylaminoadenine 0.1mg/L+kinetin 0.5mg/L+naphthaleneacetic acid 0.05 mg/L + 3% sucrose + 0.2% NaCl medium for 2 generations. Clustered shoots appear after several generations of cultivation.

4. Rooting culture: Select well-growing clustered shoots and divide them into individual plants, and transfer them to 1/2MS+indolebutyric acid 0.5mg/L+mass fraction of 2% sucrose+mass fraction of 0.3% NaCl rooting medium to induce rooting. Cultivate for 15-20 days, the root system is about 5 or more, and the seedling height is 4-6cm.

The above culture conditions in the tissue culture room are 25° C., 75% relative air humidity, 3000 Lx light intensity, and 10 hours of light per day.

5. Seedling hardening: Eliminate poorly growing tissue cultured seedlings, open the bottle of well-grown rooted seedlings, open the bottle at room temperature for 2-3 days, take out the test-tube seedlings, rinse off the root culture medium with clean water, Transplant to sand bed for seedling hardening. The bottom layer of the sand bed is about 10cm of pebbles, the middle is about 5cm of soil, and the upper layer is about 20cm of sea sand (the salt content of sea sand is about 0.4%). When hardening the seedlings, the row-to-plant spacing is 10×10 cm, and automatic sprinkler irrigation is performed irregularly according to the humidity of the soil and seedling leaves to keep the leaves of the seedlings moist without losing water. After the new roots have sprouted new leaves, they are transplanted into containers for cultivation. During the period, pay attention to the salt content in the substrate is basically kept constant, and when the salt content in the substrate decreases, add an appropriate amount of NaCl to the sprinkler water.

6. Transplanting seedlings: use sunshade nets (about 50% light transmittance) within 10 days after transplanting seedlings. The 1-year growth seedling container mesh bag container has a diameter of 4-5cm and a height of 8-10cm; the 2-year growth seedling container mesh bag container and detachable plastic container have a diameter of 18-20cm and a height of 300-310cm. Liudaomu’s 1-year-old container test substrate is peat: sea sand=7:3, slow-release compound fertilizer (1kg m-3) according to the volume ratio; the 2-year-old container substrate is peat according to the volume ratio: sea sand=6 :4, slow-release compound fertilizer (2kg.m-3). In order to prevent seedlings from being damaged by diseases and insect pests, the matrix must be strictly sterilized, and NaCl should be added when preparing the matrix. The content of NaCl in the matrix of annual seedlings is 0.5%, and the content of NaCl in the matrix of biennial seedlings is 0.6%.

7. Seedling stage management: Container seedlings are placed in multi-span greenhouses equipped with sunshade and spraying facilities. The temperature in the greenhouse is kept at 20-25°C, and the relative humidity of the soil is kept at 65-75%. Change pots after the 2nd year according to the growth status of seedlings, and when the biennial seedling matrix changes pots, the matrix is sea sand=6:4 according to the volume ratio, slow-release compound fertilizer (2kg.m-3); The NaCl content is 0.6%. The container used is a mesh bag container with a diameter of 18-20cm, a height of 300-310cm, and a potting soil volume of 800-1000g.

The air temperature is 18°C, and the soil (substrate) temperature is 15°C during the above seedling hardening, transplanting and seedling raising.

The slow-release compound fertilizer has been applied to the substrate, and generally there is no need to apply fertilizer again, but it can be combined with watering according to the growth status of the container seedlings and appropriate topdressing outside the root. During the high temperature period in summer, the container seedlings need to be shaded, and the light transmittance of the shade is about 60% of the full sunlight. If any disease occurs, remove the diseased plants in time, and use corresponding pesticides to spray and sterilize. If pests occur, control them in time. At the seedling stage, it is found that the substrate in the container sinks, and the substrate must be added in time to prevent the roots from being exposed. In seedling management, attention should also be paid to keeping the salt content in the substrate basically constant. When the salt content in the substrate decreases, add an appropriate amount of NaCl to the irrigation water.

Practice has proved that: the growth of Hexaphyllum mosaicus is hindered when the soil salt content is above 0.2%, and 0.3% is the threshold of its growth. After being treated by the method of Example 1, the annual seedlings of Hexaphyllum mosaicus with a NaCl content of 0.5% in the substrate can grow normally, and the biennial seedlings with a NaCl content of 0.6% in the substrate can grow normally.

As described above, the present invention can be preferably carried out.

Claims (8)

1. directional induction improves a method for Abelia biflora nursery stock salt resistance, it is characterized in that: comprise the following steps:

(1) material sterilizing: get Abelia biflora edible tender branch, is cut into 4-5cm stem segment with axillary bud, first in cleaning solution, soaks 5-6 minute, then cuts off the blade of expansion after rinsing 2-3 hour with water, is cut into the stem segment with axillary bud of 1.5cm; Superclean bench soaks 20 seconds with 75% alcoholic solution, and then proceeding to mass fraction is sterilizing 8-10 minute in the mercuric chloride solution of 0.1%, then uses aseptic water washing 5-8 time;

(2) induction of indefinite bud: the stem section after described step (1) sterilizing is received on No. 1 medium and cultivate 40-50 days;

Wherein, described No. 1 medium adds the NaCl that mass fraction is 0.1% on MS medium base;

(3) differentiation of bud and squamous subculture: select the good stem section of Bud polarization to receive on No. 2 medium, the bud that growth selection is good squamous subculture on same described No. 2 medium of transferring once, is then transferred on No. 3 medium and cultivated for 2 generations; Multiple Buds is there is after a few culture;

Wherein, described No. 2 medium add the NaCl that mass fraction is 0.1% on MS medium base; Described No. 3 medium add the NaCl that mass fraction is 0.2% on MS medium base;

(4) culture of rootage: choose well-grown Multiple Buds and be divided into individual plant, goes to root induction on No. 4 medium; Cultivate 15-20 days, root system more than 5, transplants seedlings during height of seedling 4-6cm; Described No. 4 medium add the NaCl that mass fraction is 0.3% on the basis of 1/2MS medium;

(5) hardening: the bottleneck of well-grown seedling of taking root is opened, at room temperature uncork hardening 2-3 days, by test-tube plantlet take out, after rinsing out root medium with clear water, be transplanted to be provided with sea sand husky bed on carry out hardening; Described sea sand salt content 0.4%; During hardening, distance between rows and hills is 10 × 10cm; New root grows and is transplanted in container and cultivates after extraction young leaves; Salt content in nurturing period chien shih sea sand is held constant at 0.4%;

(6) transplant seedlings: within 1 year, raw Abelia biflora container substrate composition is peat according to volume ratio: sea sand=7:3, slow-release compound fertilizer 1kgm ^-3; Within 2 years, raw Abelia biflora container substrate composition is peat according to volume ratio: sea sand=6:4, slow-release compound fertilizer 2kg.m ^-3; Add NaCl during preparation matrix, the NaCl content in one year seedling matrix is 0.5%, and the NaCl content in biennial seedling matrix is 0.6%;

(7) seedling management: container seedling is placed in beam-connected greenhouse, the temperature in beam-connected greenhouse remains on 20-25 DEG C, and relative moisture of the soil remains on 65-75%;

In described step (2), described No. 1 medium also adds following material: the kinetin of 0.8-1.0mg/L, the indolebutyric acid of 0.08-0.12mg/L and mass fraction are the sucrose of 3%;

In described step (3), describedly contain No. 2 medium and also add following material: methyl α-naphthyl acetate and the mass fraction of the kinetin of 0.4-0.6mg/L, the 6-benzyl aminoadenine of 0.08-0.12mg/L, 0.05mg/L are the sucrose of 3%;

In described step (3), describedly contain No. 3 medium and also add following material: methyl α-naphthyl acetate and the mass fraction of the kinetin of 0.4-0.6mg/L, the 6-benzyl aminoadenine of 0.08-0.12mg/L, 0.04-0.06mg/L are the sucrose of 3%;

In described step (4), described No. 4 medium also add following material: the indolebutyric acid of 0.4-0.6mg/L and mass fraction are the sucrose of 2%.

2. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: the condition of culture of described step (2), step (3) and step (4) is 24-25 DEG C, relative air humidity 70-75%, light intensity 2500-3000Lx, illumination 10 hours/day.

3. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (5), is the sunshade net of 50% with light transmittance within transplanting seedlings latter 10 days.

4. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, and it is characterized in that: in described step (5), described husky bed from bottom to top comprises bottom, intermediate layer and upper strata; The earth that the cobble that described bottom is 8-15cm, described intermediate layer are 5cm, described upper strata are the sea sand of 20cm.

5. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (6), and within 1 year, raw container for plant growth uses material mesh bag container, and diameter is 4-5cm, height 8-10cm; Within 2 years, raw container for plant growth uses material mesh bag container or plastic containers, and diameter is 18-20cm, is highly 300-310cm.

6. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (7), within 2nd year, carry out changing basin according to the upgrowth situation of nursery stock later, select material mesh bag container or plastic containers, diameter is 18-20cm, be highly 300-310cm, basin soil amount 800-1000g.

7. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, it is characterized in that: in described step (7), utilizes the spraying facility of beam-connected greenhouse to spray.

8. a kind of directional induction according to claim 1 improves the method for Abelia biflora nursery stock salt resistance, and it is characterized in that: in described step (7), carry out sunshade during summer high temperature to container seedling, sunshade light transmittance is 60% of full exposure.