CN103520217A - Biological bactericide and application thereof - Google Patents
Biological bactericide and application thereof Download PDFInfo
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- CN103520217A CN103520217A CN201310475599.XA CN201310475599A CN103520217A CN 103520217 A CN103520217 A CN 103520217A CN 201310475599 A CN201310475599 A CN 201310475599A CN 103520217 A CN103520217 A CN 103520217A
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- biofungicide
- essence
- nisin
- organic acid
- hydrophilic solvent
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Abstract
A biological bactericide and application thereof relates to a biological bactericide and application thereof. The biological bactericide aims at solving the problems that conventional bactericides are harmful to human body because of contained chemical components. The biological bactericide is composed of 0.2-5 g/L of nisin, 1-15 g/L of EDTA disodium salt, 50-100 g/L of glycerin, 10-200 g/L of a hydrophilic solvent, 1-10 g/L of an edible organic acid, 0.01-0.5 g/L of essence the balance purified water. The biological bactericide is used for sterilizing hands, article surfaces and public places. The biological bactericide is nontoxic and innocuous to human body, is decomposed into amino acids utilized by human body after taken by a person, and causes no damage to human body. The biological bactericide is applicable to the field of bactericides.
Description
Technical field
The present invention relates to antibacterial and application thereof, relate in particular to biological preservative nisin as the antibacterial application in hand, article surface and public place sterilization technology in daily life.
Background technology
In daily life, the sterilizing of hand is all generally the disinfectant that adds chemosterilant or add chemical composition in handwashing liquid in wet paper towel, but can cause that human body is had to harm, at present in hand sterilizing field with pure biological product nisin not also being employed as antibacterial.
Nisin is a kind of peptide material that certain streptococcus acidi lactici produces, by 34 peptide chain amino acid residues, formed, it be a kind of efficient, nontoxic, safely, the natural biological antiseptic agent that has no side effect, food source of disease bacterium and putrefactive microorganisms are had to stronger inhibitory action.Nisin concentration be 100ppm be just can kill most G
+bacterium, as staphylococcus aureus, various bacillus cereus, Streptococcus hemolyticus, listeria spp etc. and some pathogenic bacterium to harmful intestinal tract, nisin is combined use with EDETATE SODIUM also inhibited to Salmonella, escherichia coli and other gram negative bacteria.
Summary of the invention
The object of the invention is has the problem of harm in order to solve existing antibacterial because containing chemical composition to human body, a kind of biofungicide and application thereof are provided.
A kind of biofungicide of the present invention is comprised of the pure water of nisin 0.2～5g/L, EDETATE SODIUM 1～15g/L, glycerol 50～100g/L, hydrophilic solvent 10～200g/L, edible organic acid 1～10g/L, essence 0.01～0.5g/L and surplus.
Biofungicide of the present invention is used for hand, article surface and public place as antibacterial.
Described biofungicide general guide using method is as follows:
Biofungicide is directly sprayed onto to the surface of hand skin, rubs gently and make antibacterial fully and contact skin, within 2～5 minutes, after xerosis cutis, can play the effect of sterilization.
Biofungicide is directly sprayed onto to article surface, after 2～5 minutes, can plays the effect of sterilization.
The present invention comprises following beneficial effect:
1, the effect that in the present invention, nisin and EDETATE SODIUM have combined disinfection; Glycerol is Derma-Guard, mainly plays a part skin protection; Hydrophilic solvent mainly plays a part to be sprayed onto volatilization fast on skin at antibacterial; Edible organic acid mainly plays a part to regulate pH3～6; Essence mainly plays a part to regulate antibacterial abnormal smells from the patient;
2, biofungicide of the present invention is mainly comprised of natural biological preservative nisin, can effectively kill gram-positive bacterium and gram negative bacteria, for causing that pathogenic entero becteria has good killing action, and biofungicide of the present invention is nontoxic to human body, after being eaten by people, can in human body, be broken down into aminoacid and be utilized by human body, to human body, can not damage;
3, the invention provides a kind of novel antibacterial being formed by biological food antiseptic, it is mainly used in the disinfection of hand, be sprayed onto on hand, rub gently after drying and can play bactericidal effect, sterilizing rate is more than 99%, and it also can be applicable to the disinfection of the public places such as the sterilization of foodstuff processing utensil, packaging material for food and container and catering trade, hotel.
The specific embodiment
Technical solution of the present invention is not limited to the following cited specific embodiment, also comprises the combination in any between each specific embodiment.
The specific embodiment one: present embodiment biofungicide is comprised of the pure water of nisin 0.2～5g/L, EDETATE SODIUM 1～15g/L, glycerol 50～100g/L, hydrophilic solvent 10～200g/L, edible organic acid 1～10g/L, essence 0.01～0.5g/L and surplus.
The beneficial effect of present embodiment:
1, the effect that in the present invention, nisin and EDETATE SODIUM have combined disinfection; Glycerol is Derma-Guard, mainly plays a part skin protection; Hydrophilic solvent mainly plays a part to be sprayed onto volatilization fast on skin at antibacterial; Edible organic acid mainly plays a part to regulate pH3～6; Essence mainly plays a part to regulate antibacterial abnormal smells from the patient;
2, biofungicide of the present invention is mainly comprised of natural biological preservative nisin, can effectively kill gram-positive bacterium and gram negative bacteria, for causing that pathogenic entero becteria has good killing action, and biofungicide of the present invention is nontoxic to human body, after being eaten by people, can in human body, be broken down into aminoacid is utilized by human body
To human body, can not damage;
3, the invention provides a kind of novel antibacterial being formed by biological food antiseptic, it is mainly used in the disinfection of hand, be sprayed onto on hand, rub gently after drying and can play bactericidal effect, sterilizing rate is more than 99%, and it also can be applicable to the disinfection of the public places such as the sterilization of foodstuff processing utensil, packaging material for food and container and catering trade, hotel.
The specific embodiment two: present embodiment is different from the specific embodiment one: described biofungicide is comprised of the pure water of nisin 2～3g/L, EDETATE SODIUM 1～15g/L, glycerol 50～100g/L, hydrophilic solvent 10～200g/L, edible organic acid 1～10g/L, essence 0.01～0.5g/L and surplus.Other is identical with the specific embodiment one.
The specific embodiment three: present embodiment is different from the specific embodiment one or two: described biofungicide is comprised of the pure water of nisin 0.2～5g/L, EDETATE SODIUM 5～10g/L, glycerol 50～100g/L, hydrophilic solvent 10～200g/L, edible organic acid 1～10g/L, essence 0.01～0.5g/L and surplus.Other is identical with the specific embodiment one or two.
The specific embodiment four: present embodiment is different from one of specific embodiment one to three: described biofungicide is comprised of the pure water of nisin 0.2～5g/L, EDETATE SODIUM 1～15g/L, glycerol 50g/L, hydrophilic solvent 10～200g/L, edible organic acid 1～10g/L, essence 0.01～0.5g/L and surplus.Other is identical with one of specific embodiment one to three.
The specific embodiment five: present embodiment is different from one of specific embodiment one to four: described hydrophilic solvent is low-carbon (LC) monohydric alcohol, wherein said low-carbon (LC) monohydric alcohol is the edible ethanol that normal propyl alcohol or quality percentage composition are 95%.Other is identical with one of specific embodiment one to four.
The specific embodiment six: present embodiment is different from one of specific embodiment one to five: described edible organic acid is citric acid.Other is identical with one of specific embodiment one to five.
The specific embodiment seven: present embodiment is different from one of specific embodiment one to six: described essence is lemon essence, menthol or lavandula angustifolia taste essence.Other is identical with one of specific embodiment one to six.
The specific embodiment eight: the application of a kind of biofungicide of present embodiment, it is characterized in that biofungicide as antibacterial for hand, article surface and public place.
The specific embodiment nine: present embodiment is different from the specific embodiment eight: it is characterized in that described article are foodstuff processing utensil, packaging material for food and container.Other is identical with the specific embodiment eight.
The specific embodiment ten: present embodiment is different from the specific embodiment eight or nine: it is characterized in that preferably being formed by the pure water of nisin 0.2～5g/L, EDETATE SODIUM 1～15g/L, hydrophilic solvent 10～200g/L, edible organic acid 1～10g/L and surplus for the biofungicide of article surface and public place.Other is identical with the specific embodiment eight or nine.
By following examples, verify beneficial effect of the present invention:
Embodiment mono-:
Present embodiment biofungicide, is comprised of the pure water of nisin 2g/L, EDETATE SODIUM 5g/L, glycerol 50g/L, normal propyl alcohol 100g/L, citric acid 1.5g/L, lemon essence 0.05g/L and surplus.
The experiment of the present embodiment suspension method kill bacteria:
Get escherichia coli, bacillus subtilis, the culture of staphylococcus aureus in LB fluid medium, dilution coated plate detects bacteria concentration.Get 5mL biofungicide and join in test tube, add above-mentioned bacteria suspension 0.1mL, after mixing rapidly, be applied to the scheduled time, get 1mL and join dilution termination bactericidal action in 100mL sterilized water, get 1mL and do count plate.Cultivate 2 days observed results, the results are shown in Table 1 for 37 ℃.
The experiment of table 1 suspension method kill bacteria
Note: result is 3 empirical average values, and escherichia coli bacteria concentration is 3.2 * 10
8cfu/mL, staphylococcus aureus bacteria concentration is 5.8 * 10
8cfu/mL, bacillus subtilis bacteria concentration is 2.3 * 10
7cfu/mL.
The experiment of the present embodiment hand surface disinfection:
A hands getting testee contrasts, and smears the surface of hands take antibacterial with the cotton swab of putting into sterilized water after sterilizing, and the cotton swab of adopting bacterium is put into an aseptic triangular flask.Biofungicide is sprayed onto to the surface of another hands, after the effect regular hour, with the surface that the cotton swab of putting into sterilized water after sterilizing is smeared hands, takes antibacterial again, the cotton swab of adopting bacterium is put into another aseptic triangular flask.Bacterium is adopted with 2 cotton swabs in the surface of each hands, and every group of experiment got 30 people and tested, and the bacterium cotton swab unification of adopting of matched group is put into an aseptic triangular flask, and another aseptic triangular flask is put in the bacterium cotton swab unification of adopting of test group.With physiological saline solution, dilute respectively different gradients and be down flat respectively plate, be placed in incubator and cultivate 2 days,
Observed result, record data, calculate sterilizing rate, the results are shown in Table 2.
Table 2 hand surface disinfection experimental result
Embodiment bis-:
Present embodiment biofungicide, the pure water of the edible ethanol 200g/L that is 95% by nisin 5g/L, EDETATE SODIUM 10g/L, glycerol 50g/L, quality percentage composition, citric acid 1.5g/L, lemon essence 0.05g/L and surplus forms.
The experiment of the present embodiment suspension method kill bacteria:
Get escherichia coli, bacillus subtilis, the culture of staphylococcus aureus in LB fluid medium, dilution coated plate detects bacteria concentration.Get 5mL biofungicide and join in test tube, add above-mentioned bacteria suspension 0.1mL, after mixing rapidly, be applied to the scheduled time, get 1mL and join dilution termination bactericidal action in 100mL sterilized water, get 1mL and do count plate.Cultivate 2 days observed results, the results are shown in Table 3 for 37 ℃.
The experiment of table 3 suspension method kill bacteria
Note: result is 3 empirical average values, and escherichia coli bacteria concentration is 3.2 * 10
8cfu/mL, staphylococcus aureus bacteria concentration is 5.8 * 10
8cfu/mL, bacillus subtilis bacteria concentration is 2.3 * 10
7cfu/mL.
The experiment of hand surface disinfection:
A hands getting testee contrasts, and smears the surface of hands take antibacterial with the cotton swab of putting into sterilized water after sterilizing, and the cotton swab of adopting bacterium is put into an aseptic triangular flask.Biofungicide is sprayed onto to the surface of another hands, after the effect regular hour, with the surface that the cotton swab of putting into sterilized water after sterilizing is smeared hands, takes antibacterial again, the cotton swab of adopting bacterium is put into another aseptic triangular flask.Bacterium is adopted with 2 cotton swabs in the surface of each hands, and every group of experiment got 30 people and tested, and the bacterium cotton swab unification of adopting of matched group is put into an aseptic triangular flask, and another aseptic triangular flask is put in the bacterium cotton swab unification of adopting of test group.With physiological saline solution, dilute respectively different gradients and be down flat respectively plate, be placed in incubator and cultivate 2 days, observed result, record data, calculate sterilizing rate, the results are shown in Table 4.
Table 4 hand surface disinfection experimental result
With this biofungicide, hand is sterilized, spray function after 2 minutes germicidal efficiency more than 99%, be a kind of extraordinary nontoxic daily life antibacterial.
Claims (10)
1. a biofungicide, is characterized in that what biofungicide was comprised of the pure water of nisin 0.2～5g/L, EDETATE SODIUM 1～15g/L, glycerol 50～100g/L, hydrophilic solvent 10～200g/L, edible organic acid 1～10g/L, essence 0.01～0.5g/L and surplus.
2. a kind of biofungicide according to claim 1, is characterized in that what described biofungicide was comprised of the pure water of nisin 2～3g/L, EDETATE SODIUM 1～15g/L, glycerol 50～100g/L, hydrophilic solvent 10～200g/L, edible organic acid 1～10g/L, essence 0.01～0.5g/L and surplus.
3. a kind of biofungicide according to claim 1, is characterized in that what described biofungicide was comprised of the pure water of nisin 0.2～5g/L, EDETATE SODIUM 5～10g/L, glycerol 50～100g/L, hydrophilic solvent 10～200g/L, edible organic acid 1～10g/L, essence 0.01～0.5g/L and surplus.
4. a kind of biofungicide according to claim 1, is characterized in that what described biofungicide was comprised of the pure water of nisin 0.2～5g/L, EDETATE SODIUM 1～15g/L, glycerol 50g/L, hydrophilic solvent 10～200g/L, edible organic acid 1～10g/L, essence 0.01～0.5g/L and surplus.
5. a kind of biofungicide according to claim 1, is characterized in that described hydrophilic solvent is low-carbon (LC) monohydric alcohol, and wherein said low-carbon (LC) monohydric alcohol is the edible ethanol that normal propyl alcohol or quality percentage composition are 95%.
6. a kind of biofungicide according to claim 1, is characterized in that described edible organic acid is citric acid.
7. a kind of biofungicide according to claim 1, is characterized in that described essence is lemon essence, menthol or lavandula angustifolia taste essence.
8. an application for biofungicide, it is characterized in that biofungicide as antibacterial for hand, article surface and public place.
9. the application of a kind of biofungicide according to claim 8, is characterized in that described article are foodstuff processing utensil, packaging material for food and container.
10. the application of a kind of biofungicide according to claim 8, is characterized in that preferably the pure water of nisin 0.2～5g/L, EDETATE SODIUM 1～15g/L, hydrophilic solvent 10～200g/L, edible organic acid 1～10g/L and surplus, consisting of for the biofungicide of article surface and public place.
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| CN201310475599.XA CN103520217A (en) | 2013-10-12 | 2013-10-12 | Biological bactericide and application thereof |
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| CN201310475599.XA CN103520217A (en) | 2013-10-12 | 2013-10-12 | Biological bactericide and application thereof |
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Cited By (2)
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| CN106261464A (en) * | 2016-08-17 | 2017-01-04 | 安泰生物工程股份有限公司 | A formula for expanding the bactericidal range and improving bactericidal ability of nisin |
| CN107261125A (en) * | 2017-06-09 | 2017-10-20 | 太仓苏浏水产有限公司 | A kind of fish bactericide |
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| CN101720969A (en) * | 2009-11-13 | 2010-06-09 | 合肥工业大学 | Composite preservative for fruits, flour products and bean products |
| CN101965867A (en) * | 2010-08-26 | 2011-02-09 | 天津市食品加工工程中心 | Special biological preservative for honey pomelos and navel oranges |
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106261464A (en) * | 2016-08-17 | 2017-01-04 | 安泰生物工程股份有限公司 | A formula for expanding the bactericidal range and improving bactericidal ability of nisin |
| CN107261125A (en) * | 2017-06-09 | 2017-10-20 | 太仓苏浏水产有限公司 | A kind of fish bactericide |
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Application publication date: 20140122 |