CN103519160B - Puffed grifola frondosa and preparation method thereof - Google Patents
Puffed grifola frondosa and preparation method thereof Download PDFInfo
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Abstract
本发明公开了一种膨化灰树花产品及其生产方法。该方法包括如下步骤:将灰树花原料放置于膨化罐中进行变温压差膨化,得到所述膨化灰树花产品。所述变温压差膨化包括:1)将所述灰树花原料于膨化罐中在温度为90-120℃,在常压状态下保持不大于15分钟;2)保持体系的温度与步骤1)相同,将体系降压至真空状态,在所述常压状态和真空状态之间连续转换3-5次后,在真空状态降温至60-80℃保持146-150分钟;3)保持体系的真空度与步骤2)相同,将体系继续降温至10-20℃保持30-50分钟后破除真空。该方法在加工过程中不添加色素和其他添加剂等,尽量保留了灰树花的颜色和风味,纯净天然,安全卫生。The invention discloses a puffed grifola frondosa product and a production method thereof. The method comprises the following steps: placing the raw material of the frondosa frondosa in an expansion tank to carry out puffing with variable temperature and pressure difference to obtain the puffed frondosa frondosa product. The temperature-variable-pressure-difference expansion includes: 1) Keeping the Grifola frondosa raw material in an expansion tank at a temperature of 90-120°C under normal pressure for no more than 15 minutes; 2) Keeping the temperature of the system and step 1) In the same way, depressurize the system to a vacuum state, and after continuously switching between the normal pressure state and the vacuum state for 3-5 times, cool down to 60-80°C in the vacuum state and keep it for 146-150 minutes; 3) Keep the vacuum of the system The temperature is the same as step 2), continue to cool the system to 10-20°C and keep it for 30-50 minutes, then break the vacuum. The method does not add pigments and other additives in the processing process, keeps the color and flavor of the frondosa frondosa as much as possible, is pure and natural, safe and hygienic.
Description
技术领域technical field
本发明属于食品加工领域,涉及一种膨化灰树花及其制备方法。The invention belongs to the field of food processing, and relates to puffed frondosa frondosa and a preparation method thereof.
背景技术Background technique
灰树花[Grifola frondosa]隶属于担子菌亚门,层菌纲,非褶菌目,多孔菌科,树花菌属,又名栗蘑、贝叶多孔菌、千佛菌、云蕈、莲花菌等,日本称之舞茸,美国称之林鸡。灰树花是世界上一种优良的食、药两用真菌。灰树花鲜品具有独特清香味,滋味鲜美;干品具有浓郁的芳香味,肉质嫩脆,味如鸡丝,脆似玉兰。灰树花营养价值极高,经测干菇含蛋白质24.6%,脂肪2.42%,碳水化合物47.5%(其中粗纤维11%),水分18.4%,含18种氨基酸,富含人体必需的9种氨基酸,异亮氨酸、亮氨酸、赖氨酸含量均高于其他食用菌;此外灰树花含有丰富的维生素(维生素C、B2、D2、E、烟酸等)和微量元素(如钾、钙、镁、铁、磷、硒、锌等),其含量也高于其他食用菌。Grifola frondosa [Grifola frondosa] belongs to Basidiomycotina, Phyllomycetes, Phyllomycetes, Polyporaceae, Tree Flower Fungus, also known as Chestnut Mushroom, Bayeux Polypore, Thousand Buddha Fungus, Cloud Mushroom, Lotus Fungus etc., Japan calls it Maitake, and the United States calls it Forest Chicken. Grifola frondosa is an excellent edible and medicinal fungus in the world. The fresh product of Grifola frondosa has a unique fragrance and delicious taste; the dried product has a strong aroma, the meat is tender and crisp, tastes like shredded chicken, and is as crisp as magnolia. The nutritional value of Grifola frondosa is very high. According to the test, the dried mushroom contains 24.6% protein, 2.42% fat, 47.5% carbohydrate (including 11% crude fiber), 18.4% water, contains 18 kinds of amino acids, and is rich in 9 kinds of amino acids necessary for human body , the contents of isoleucine, leucine and lysine are higher than other edible fungi; in addition, Grifola frondosa is rich in vitamins (vitamin C, B2, D2, E, niacin, etc.) and trace elements (such as potassium, Calcium, magnesium, iron, phosphorus, selenium, zinc, etc.), its content is also higher than other edible fungi.
灰树花具有极高的医疗保健功能。据大量文献报道,灰树花多糖具有多种药理活性,其中以D组分活性最为明显。灰树花多糖D组分具有改善免疫系统功能、调节血糖血脂及胆固醇水平、降血压等作用,对艾滋病、高血压、肥胖症、糖尿病以及免疫系统功能的紊乱都有一定的疗效。此外,D组分还可以抑制癌细胞的生长和转移,具有明显的抗肿瘤作用。因此灰树花是一种珍贵的食用菌,被誉为“食用菌王子”和“华北人参”。Grifola frondosa has extremely high health care functions. According to a large number of literature reports, Grifola frondosa polysaccharide has various pharmacological activities, among which the activity of component D is the most obvious. Grifola frondosa polysaccharide D component has the effects of improving immune system function, regulating blood sugar, blood lipid and cholesterol levels, lowering blood pressure, etc. It has certain curative effects on AIDS, hypertension, obesity, diabetes and immune system dysfunction. In addition, D component can also inhibit the growth and metastasis of cancer cells, and has obvious anti-tumor effect. Therefore, Grifola frondosa is a precious edible fungus, known as "Prince of Edible Fungi" and "North China Ginseng".
目前灰树花多为烹调食用,市场上关于灰树花的加工产品较少且形式单一,普遍为脱水干制品。生产绿色天然即食的食用菌脆片是食用菌加工产业的新趋势。食品脆片加工技术主要有真空油炸、微波干燥及冷冻干燥等,真空油炸会产生对人体有害的物质,并且产品中有油脂残留;微波干燥和冷冻干燥加工的产品虽然性状较好,但是口感较差且加工设备投入费用高,能源消耗大,不利于推广及大规模生产。At present, Grifola frondosa is mostly eaten for cooking. There are few processed products of Grifola frondosa on the market and the form is single, and they are generally dehydrated dry products. The production of green, natural and ready-to-eat mushroom chips is a new trend in the mushroom processing industry. Food chips processing technologies mainly include vacuum frying, microwave drying and freeze drying. The taste is poor and the input cost of processing equipment is high, and the energy consumption is large, which is unfavorable for popularization and large-scale production.
发明内容Contents of the invention
本发明的目的是提供一种膨化灰树花及其制备方法。The object of the present invention is to provide a kind of puffed frondosa frondosa and its preparation method.
本发明提供的制备膨化灰树花的方法,包括如下步骤:将灰树花原料置于膨化罐中进行变温压差膨化,得到所述膨化灰树花。The method for preparing puffed Grifola frondosa provided by the invention comprises the following steps: placing the raw material of Grifola frondosa in an expansion tank for variable temperature and pressure difference puffing to obtain the puffed Grifola frondosa.
上述方法中,所述变温压差膨化包括如下步骤:In the above method, the variable temperature and pressure difference expansion includes the following steps:
1)将所述灰树花原料于膨化罐中在温度为90-120℃,在常压状态下保持不大于15分钟;1) keeping the Grifola frondosa raw material in an expansion tank at a temperature of 90-120°C under normal pressure for no more than 15 minutes;
2)保持体系的温度与步骤1)相同,将体系降压至真空状态,在所述常压状态和真空状态之间连续转换3-5次后,在真空状态降温至60-80℃保持146-150分钟;2) Keep the temperature of the system the same as step 1), reduce the pressure of the system to a vacuum state, and switch between the normal pressure state and the vacuum state for 3-5 times, then cool down to 60-80°C in the vacuum state and keep for 146 -150 minutes;
3)保持体系的真空度与步骤2)相同,将体系继续降温至10-20℃保持30-50分钟后破除真空。3) Keep the vacuum of the system the same as step 2), continue to cool the system to 10-20°C for 30-50 minutes and then break the vacuum.
上述方法的步骤1)中,所述温度优选为105℃;所述保持时间优选为10分钟;In step 1) of the above method, the temperature is preferably 105°C; the holding time is preferably 10 minutes;
步骤2)中,所述真空状态的真空度为0至-0.1MPa,优选-0.098MPa;所述转换的次数优选为4次;所述降温步骤中,降温后的温度优选为70℃,降温所用时间为1-3分钟,优选2分钟;保持时间优选为148分钟;压强下降是通过真空罐抽真空瞬间降压至真空状态。In step 2), the vacuum degree of the vacuum state is 0 to -0.1MPa, preferably -0.098MPa; the number of conversions is preferably 4 times; in the cooling step, the temperature after cooling is preferably 70°C, and the cooling The time used is 1-3 minutes, preferably 2 minutes; the holding time is preferably 148 minutes; the pressure drop is instantaneously reduced to a vacuum state by vacuuming the vacuum tank.
所述步骤2)具体可为如下步骤a、b或c:The step 2) can specifically be the following steps a, b or c:
其中,步骤a包括:保持体系的温度与步骤1)相同,将体系降压至真空状态后,再立即恢复至常压状态,再将体系降压至真空状态后,立即恢复至常压状态,再将体系降压至真空状态后,在真空状态降温至60-80℃保持146-150分钟;Among them, step a includes: keeping the temperature of the system the same as step 1), reducing the pressure of the system to a vacuum state, and then immediately returning to a normal pressure state, and then reducing the pressure of the system to a vacuum state, and immediately returning to a normal pressure state, After the system is depressurized to a vacuum state, the temperature is lowered to 60-80°C in a vacuum state and kept for 146-150 minutes;
步骤b包括:保持体系的温度与步骤1)相同,将体系降压至真空状态后,再立即恢复至常压状态,再将体系降压至真空状态后,立即恢复至常压状态,再将体系降压至真空状态后,再立即恢复至常压状态,再将体系降压至真空状态后,在真空状态降温至60-80℃保持146-150分钟;Step b includes: keeping the temperature of the system the same as step 1), reducing the pressure of the system to a vacuum state, and then immediately returning to a normal pressure state, and then reducing the system to a vacuum state, immediately returning to a normal pressure state, and then After the system is depressurized to a vacuum state, it is immediately returned to a normal pressure state, and then the system is depressurized to a vacuum state, and the temperature is lowered to 60-80°C in a vacuum state for 146-150 minutes;
步骤c包括:保持体系的温度与步骤1)相同,将体系降压至真空状态后,再立即恢复至常压状态,再将体系降压至真空状态后,立即恢复至常压状态,再将体系降压至真空状态后,再立即恢复至常压状态,再将体系降压至真空状态后,再立即恢复至常压状态,再将体系降压至真空状态后,在真空状态降温至60-80℃保持146-150分钟;Step c includes: keeping the temperature of the system the same as step 1), reducing the pressure of the system to a vacuum state, and then immediately returning to a normal pressure state, and then reducing the system to a vacuum state, immediately returning to a normal pressure state, and then After the system is depressurized to a vacuum state, it is immediately returned to a normal pressure state, and then the system is depressurized to a vacuum state, and then immediately returned to a normal pressure state, and then the system is depressurized to a vacuum state, and then cooled to 60°C in a vacuum state. -80°C for 146-150 minutes;
步骤3)中,降温后的温度优选为15℃,保持的时间优选为40分钟。In step 3), the temperature after cooling is preferably 15°C, and the holding time is preferably 40 minutes.
所述步骤3)降温步骤中,降温的方式均选自自然冷却和空调冷却中的至少一种。In the step 3) in the cooling step, the cooling method is selected from at least one of natural cooling and air-conditioning cooling.
所述破除真空步骤为将体系由真空状态立即恢复至常压状态;如可通过关闭真空阀,打开大气阀实现该破除真空的步骤;The step of breaking the vacuum is to return the system to the normal pressure state immediately from the vacuum state; as can realize the step of breaking the vacuum by closing the vacuum valve and opening the atmospheric valve;
所述方法还包括:在所述变温压差膨化步骤之前将所述灰树花原料进行预干燥至其中水的质量百分含量为18-22%,具体为19.4%、20.3%、21.1%、19.4-21.1%、19.4-20.3%或20.3-21.1%。The method also includes: before the variable temperature and pressure difference puffing step, pre-drying the Grifola frondosa raw material until the mass percentage of water therein is 18-22%, specifically 19.4%, 20.3%, 21.1%, 19.4-21.1%, 19.4-20.3%, or 20.3-21.1%.
其中,所述预干燥步骤中,温度为70-90℃,优选80℃,时间为3-5h,优选4h。Wherein, in the pre-drying step, the temperature is 70-90°C, preferably 80°C, and the time is 3-5h, preferably 4h.
所述方法还包括:The method also includes:
在所述预干燥步骤之后,所述变温压差膨化步骤之前,将与预干燥后的灰树花原料于密闭容器中贮存24h。After the pre-drying step and before the variable temperature and pressure difference puffing step, store the pre-dried Grifola frondosa raw material in an airtight container for 24 hours.
所述方法还包括:The method also includes:
在所述预干燥步骤之前,将所述灰树花原料于100℃的水中热烫5分钟。Before the pre-drying step, the Grifola frondosa raw material was blanched in water at 100° C. for 5 minutes.
上述灰树花原料是将灰树花进行撕分和清洗。The above-mentioned Grifola frondosa raw material is that Grifola frondosa is torn apart and cleaned.
上述方法适用所有品种的灰树花。The above method is applicable to all varieties of Grifola frondosa.
按照上述方法制备得到膨化灰树花产品及含有所述膨化灰树花产品的产品,也属于本发明的保护范围。The expanded Grifola frondosa product and products containing the expanded Grifola frondosa product prepared according to the above method also belong to the protection scope of the present invention.
本发明的方法采用预干燥和变温压差膨化干燥的方法制成膨化灰树花。在反复膨化过程中,灰树花原料因组织内部水分瞬间汽化而散失,导致原料细胞内或细胞间膨胀形成均匀的蜂窝状结构,使产品有很好的酥脆性,口感好,易消化。这种均匀多孔的质地也使产品易于吸水,有良好的复水性。产品在加工过程中不添加任何色素及添加剂,尽量保留了灰树花的颜色和风味,纯净天然,安全卫生。The method of the present invention adopts the method of pre-drying and variable temperature and pressure difference puffing and drying to prepare puffed frondosa frondosa. During the repeated puffing process, the raw material of Grifola frondosa is lost due to the instant vaporization of the moisture inside the tissue, resulting in the expansion of the raw material cells or between cells to form a uniform honeycomb structure, which makes the product have good crispness, good taste and easy digestion. This uniform and porous texture also makes the product easy to absorb water and has good rehydration properties. The product does not add any pigments and additives in the process of processing, try to retain the color and flavor of Grifola frondosa, pure and natural, safe and hygienic.
本发明的膨化灰树花能较好地保留灰树花中的多种营养成份,如可溶性糖、纤维素、蛋白质以及灰树花多糖D组分。本发明的膨化灰树花含水量一般在3%-5%,使膨化产品的水分活度较低,不利于微生物生长繁殖,可以长期保存。另外,采用变温压差膨化的灰树花在加工过程中没有经过高温油炸处理,产品中没有油脂的残留,这是与油炸处理的产品最大的不同,也是本产品最大的优势,并且产品克服了油炸产品因含有少量油脂而不易贮存、风味易变劣等缺点。The puffed frondosa frondosa of the invention can better retain various nutritional components in the frondosa frondosa, such as soluble sugar, cellulose, protein and component D of the frondosa frondosa polysaccharide. The water content of the puffed Grifola frondosa of the present invention is generally 3%-5%, so that the water activity of the puffed product is low, which is not conducive to the growth and reproduction of microorganisms, and can be stored for a long time. In addition, the Grifola frondosa expanded with variable temperature and pressure difference has not undergone high-temperature frying treatment during the processing process, and there is no oil residue in the product. This is the biggest difference from fried products, and it is also the biggest advantage of this product. It overcomes the disadvantages of fried products that are not easy to store because they contain a small amount of oil, and the flavor is easy to deteriorate.
本发明采用变温压差膨化干燥方法,对灰树花原料直接进行烘干、膨化,最大程度的保持了原料的营养成份和风味。膨化设备与冷冻干燥设备相比具有适用性广、价格低、操作简单、易于控制等特点,这些优势有利于变温压差膨化技术的推广和使用。The invention adopts the puffing and drying method with variable temperature and pressure difference to directly dry and puff the raw material of the frondosa frondosa, so as to maintain the nutritional components and flavor of the raw material to the greatest extent. Compared with freeze-drying equipment, puffing equipment has the characteristics of wide applicability, low price, simple operation, and easy control. These advantages are conducive to the promotion and use of variable temperature and pressure difference puffing technology.
本发明提供的膨化灰树花产品,可直接作为天然、绿色的膨化休闲食品或早餐辅助食品,携带和食用方便;膨化灰树花可以作为方便食品,如方便面的添加料;另外,这种膨化产品也可以进一步经过超微粉碎后生产新型食用菌营养粉,作为生产新型功能食品(尤其适用于儿童或老年人)的原料;灰树花超微粉也可以直接调配作为健康饮品。The puffed frondosa frondosa product provided by the invention can be directly used as a natural and green puffed snack food or breakfast supplementary food, which is convenient to carry and eat; puffed frondosa frondosa can be used as an instant food, such as an additive for instant noodles; The product can also be further ultrafinely pulverized to produce new edible fungus nutritional powder, which can be used as a raw material for producing new functional foods (especially suitable for children or the elderly); the superfine powder of Grifola frondosa can also be directly formulated as a health drink.
具体实施方式Detailed ways
下面结合具体实施例对本发明作进一步阐述,但本发明并不限于以下实施例。所述方法如无特别说明均为常规方法。所述原材料如无特别说明均能从公开商业途径而得。所述百分含量,如无特别说明,均为质量百分含量。The present invention will be further described below in conjunction with specific examples, but the present invention is not limited to the following examples. The methods are conventional methods unless otherwise specified. The raw materials can be obtained from open commercial channels unless otherwise specified. Said percentages are all percentages by mass unless otherwise specified.
实施例1、膨化灰树花Embodiment 1, expanded Grifola frondosa
一、膨化灰树花的制备One, the preparation of puffed Grifola frondosa
取新鲜灰树花作为原料,按照下述方法测定鲜样灰树花原料的各项指标:Get fresh Grifola frondosa as raw material, measure each index of fresh sample Grifola frondosa raw material according to the following methods:
水分测定方法:干燥法。具体为:精确称量样品2-10g,置已干燥,冷却并称至恒重的有盖的称量瓶中,移入95-105℃的常压烘箱中,开盖烘2-4h后取出,加盖置于干燥器内冷却0.5h后称重。再烘一小时后称重。重复此操作,直至前后两次质量差不超过2mg即为干燥后恒重,水分含量=(鲜重(干燥前重量)-干燥后恒重)/鲜重。Moisture determination method: drying method. Specifically: accurately weigh 2-10g of the sample, place it in a covered weighing bottle that has been dried, cooled and weighed to constant weight, move it into a normal pressure oven at 95-105°C, open the cover and bake for 2-4 hours, then take it out. Covered and placed in a desiccator to cool for 0.5h and then weighed. Weigh after baking for another hour. Repeat this operation until the mass difference between the two times before and after does not exceed 2 mg, which is the constant weight after drying, and the water content = (fresh weight (weight before drying) - constant weight after drying)/fresh weight.
蛋白质测定方法:按照国家标准GB8856-88,水果、蔬菜产品粗蛋白质测定方法测定蛋白质含量。Protein determination method: Determination of protein content according to the national standard GB8856-88, determination method of crude protein of fruit and vegetable products.
粗纤维测定方法:纤维素测定方法:按照国家标准GB10469-89,水果、蔬菜粗纤维的测定方法(重量法)测定纤维素含量。Determination method of crude fiber: Determination method of cellulose: Determination of cellulose content according to the national standard GB10469-89, Determination method of crude fiber of fruits and vegetables (weight method).
可溶性糖测定方法:按照国家标准GB6194-86水果、蔬菜可溶性测定方法测定可溶性糖含量。仪器与试剂配制,同GB6194-86。Determination method of soluble sugar: determine the content of soluble sugar according to the national standard GB6194-86 Determination method of soluble sugar in fruits and vegetables. The preparation of instruments and reagents is the same as GB6194-86.
灰树花多糖D组分的提取方法:将灰树花鲜样原料放在热风干燥箱(上海精宏实验设备有限公司,电热恒温鼓风干燥箱:DHG-9123A型)中进行干燥,干燥温度为80℃,干燥12h后,用植物粉碎机将灰树花原料粉碎成粉末;膨化灰树花产品直接用植物粉碎机粉碎成粉末。100g灰树花干粉用1000mL沸水抽提30min后,过滤取上清液,加入等体积乙醇4℃放置过夜后,过滤得沉淀,用水重悬,加入乙醇使其终浓度为80%,4℃放置过夜后,8000rpm离心15min,得到沉淀即为灰树花多糖D组分粗品,经DEAE离子交换层析后,浓缩干燥,得到灰树花多糖D组分。Extraction method of Grifola frondosa polysaccharide D component: put the fresh material of Grifola frondosa frondosa in a hot air drying oven (Shanghai Jinghong Experimental Equipment Co., Ltd., electric heating constant temperature blast drying oven: DHG-9123A type) to dry, drying temperature After drying at 80°C for 12 hours, the raw material of Grifola frondosa is crushed into powder with a plant grinder; the puffed Grifola frondosa product is directly crushed into powder with a plant grinder. After extracting 100g of Grifola frondosa dry powder with 1000mL boiling water for 30min, filter the supernatant, add an equal volume of ethanol and place it overnight at 4°C, filter the precipitate, resuspend it with water, add ethanol to make the final concentration 80%, and place it at 4°C After overnight, centrifuge at 8000rpm for 15min to obtain a precipitate which is the crude product of Grifola frondosa polysaccharide D component, which is subjected to DEAE ion exchange chromatography, concentrated and dried to obtain Grifola frondosa polysaccharide D component.
上述各项指标检测结果为:灰树花鲜样含水量为85.32%,含蛋白质30.84%,粗纤维9.48%,可溶性糖39.96%,100g灰树花鲜样粉末提取出灰树花多糖D组分253mg。The test results of the above indicators are: the water content of the fresh sample of Grifola frondosa is 85.32%, the protein content is 30.84%, the crude fiber is 9.48%, and the soluble sugar is 39.96%. 253 mg.
选取新鲜灰树花原料,取出劣质、杂质后,将灰树花撕分、清洗后,按照如下方法制备膨化灰树花:Select fresh Grifola frondosa raw materials, remove inferior quality and impurities, tear apart and clean Grifola frondosa, and prepare puffed Grifola frondosa according to the following method:
1、预处理(热烫):用100℃热水热烫5分钟。1. Pretreatment (hot blanching): blanching with 100°C hot water for 5 minutes.
2、预干燥:把步骤1预处理后的灰树花原料放在热风干燥箱(上海精宏实验设备有限公司,电热恒温鼓风干燥箱:DHG-9123A型)中进行预干燥,干燥温度为80℃,干燥时间4h。按照上述的干燥法测定含水量,结果表明,干燥后的灰树花的含水量为19.4%。2, pre-drying: put the Grifola frondosa raw material after the pretreatment of step 1 in the hot-air drying oven (Shanghai Jinghong Experimental Equipment Co., Ltd., electric heating constant temperature blast drying oven: DHG-9123A type) and carry out pre-drying, the drying temperature is 80°C, drying time 4h. Measure water content according to above-mentioned drying method, the result shows, the water content of dried frondosa frondosa is 19.4%.
3、均湿:把步骤2预干燥处理后的灰树花原料进行均湿处理,即将预干燥处理后的原料放在密闭容器中室温贮存24h。3. Humidity equalization: the pre-dried Grifola frondosa raw material in step 2 is subjected to a humidity equalization treatment, that is, the pre-dried raw material is stored in an airtight container at room temperature for 24 hours.
4、变温压差膨化干燥4. Puffing and drying with variable temperature and pressure difference
工艺流程:预干燥的灰树花原料摆盘装入膨化罐、封罐、加温加压、泄压膨化、变温真空脱水、冷却、开罐。Technological process: put the pre-dried Grifola frondosa raw materials into the expansion tank, seal the tank, heat and pressurize, release pressure and expand, change temperature and vacuum dehydration, cool, and open the tank.
具体步骤为:The specific steps are:
1)将步骤3均湿后的灰树花原料放置于膨化罐(QDPH10-1型,天津市勤德新材料科技有限公司)中,加温至温度为105℃,常压保持10分钟;1) Place the Grifola frondosa raw material wetted in step 3 in an expansion tank (QDPH10-1 type, Tianjin Qinde New Material Technology Co., Ltd.), heat it to a temperature of 105°C, and keep it at normal pressure for 10 minutes;
2)再打开真空阀将膨化罐与真空罐连通,抽真空,使压强瞬间降为真空度为-0.098MPa的真空状态;2) Then open the vacuum valve to connect the expansion tank with the vacuum tank, and pump the vacuum to reduce the pressure to a vacuum state with a vacuum degree of -0.098MPa;
关闭真空阀,打开大气阀,使体系恢复常压;Close the vacuum valve, open the atmospheric valve, and restore the system to normal pressure;
再立即打开真空阀,使体系瞬间降为-0.098MPa的真空状态;Then open the vacuum valve immediately, so that the system is instantly reduced to a vacuum state of -0.098MPa;
关闭真空阀,打开大气阀,使体系恢复常压;Close the vacuum valve, open the atmospheric valve, and restore the system to normal pressure;
再立即打开真空阀,使体系瞬间降为-0.098MPa的真空状态;Then open the vacuum valve immediately, so that the system is instantly reduced to a vacuum state of -0.098MPa;
关闭真空阀,打开大气阀,使体系恢复常压;Close the vacuum valve, open the atmospheric valve, and restore the system to normal pressure;
再立即打开真空阀,使体系瞬间降为-0.0098MPa的真空状态,保持该压强,将温度在2分钟的时间内降至70℃,然后保持148分钟,使灰树花原料含水量为3-5%;Open the vacuum valve immediately again to make the system drop to the vacuum state of -0.0098MPa in an instant, keep the pressure, drop the temperature to 70°C in 2 minutes, then keep it for 148 minutes, so that the water content of the Grifola frondosa raw material is 3- 5%;
3)在步骤2)之后,将体系温度先自然冷却至室温再空调冷却至15℃,保持40分钟后破除真空,得到本发明提供的膨化灰树花。3) After step 2), the temperature of the system is naturally cooled to room temperature and then air-conditioned to 15°C, and the vacuum is broken after keeping for 40 minutes to obtain the puffed Grifola frondosa provided by the present invention.
5、分级、包装:将膨化灰树花分级采用手工或自动分级机;包装采用充氮包装。5. Grading and packaging: the puffed maitake frondosa is classified by manual or automatic grading machine; the packaging is packed with nitrogen.
二、膨化灰树花产品的品质检测2. Quality inspection of puffed Grifola frondosa products
1、感官检测1. Sensory testing
将被测样品倒在洁净的白瓷盘中,用肉眼直接观察色泽,形态和杂质,嗅其气味,品尝滋味,结果表明上述制备的膨化灰树花保留了原料的颜色和风味;原料细胞内或细胞间膨胀形成均匀的蜂窝状结构,有很好的酥脆性,口感好,易消化,所得结果如表1所示。Pour the sample to be tested into a clean white porcelain plate, directly observe the color, shape and impurities with the naked eye, smell its smell, taste the taste, the results show that the above prepared puffed Grifola frondosa retains the color and flavor of the raw material; Or the cells expand to form a uniform honeycomb structure, which has good crispness, good taste and easy digestion. The obtained results are shown in Table 1.
表1.感官检测结果Table 1. Sensory test results
2、理化指标的检测2. Detection of physical and chemical indicators
按照前述方法检测制备所得膨化灰树花中的蛋白质含量、粗纤维含量、可溶性糖含量、D组分含量。结果表明上述膨化灰树花的含水量为3.32%,蛋白质含量为28.74%,粗纤维含量为8.21%,可溶性糖含量为36.03%,100g膨化灰树花粉末制得灰树花多糖D组分212mg。The protein content, crude fiber content, soluble sugar content, and D component content in the prepared puffed Grifola frondosa were detected according to the aforementioned method. The results show that the water content of the above-mentioned expanded Grifola frondosa is 3.32%, the protein content is 28.74%, the crude fiber content is 8.21%, and the soluble sugar content is 36.03%. 100g of expanded Grifola frondosa powder makes 212mg of Grifola frondosa polysaccharide D component .
可知,该膨化灰树花能较好地保留并浓缩鲜灰树花中的多种营养成份,如可溶性糖、蛋白质、纤维素等。It can be seen that the expanded Grifola frondosa can better retain and concentrate various nutritional components in fresh Grifola frondosa, such as soluble sugar, protein, cellulose and the like.
3、贮藏期内感官与理化指标检测3. Detection of sensory and physical and chemical indicators during storage
将前述方法检测制备所得包装后的膨化灰树花在常温放置12个月后,检测各项指标,得到其含水量为3.34%,蛋白质含量为28.72%,粗纤维含量为8.20%,可溶性糖含量为36.01%,100g膨化灰树花粉末制得灰树花多糖D组分210mg。After the packaged puffed frondosa frondosa prepared by the aforementioned method was detected and prepared at room temperature for 12 months, various indicators were detected, and the water content obtained was 3.34%, the protein content was 28.72%, the crude fiber content was 8.20%, and the soluble sugar content 36.01%, 100g of puffed Grifola frondosa powder made 210mg of Grifola frondosa polysaccharide D component.
结果表明,该膨化灰树花在常温放置12个月后,其色泽、滋味和口感、形状、理化指标与刚制备所得产品相比均无明显变化。The results showed that the color, taste, mouthfeel, shape, and physical and chemical indexes of the puffed frondosa frondosa had no obvious changes compared with those of the freshly prepared product after being placed at room temperature for 12 months.
实施例2、膨化灰树花Embodiment 2, expanded Grifola frondosa
一、膨化灰树花的制备One, the preparation of puffed Grifola frondosa
取新鲜灰树花作为原料,按照实施例1所述方法进行检测,所得检测结果为:灰树花鲜样含水量为85.82%,含蛋白质31.05%,粗纤维9.76%,可溶性糖40.03%,100g灰树花鲜样粉末提取出灰树花多糖D组分246mg。Get fresh Grifola frondosa as raw material, detect according to the method described in Example 1, and the obtained detection result is: Grifola frondosa fresh sample water content is 85.82%, contains protein 31.05%, crude fiber 9.76%, soluble sugar 40.03%, 100g The Grifola frondosa fresh sample powder extracts 246 mg of the Grifola frondosa polysaccharide D component.
选取新鲜灰树花原料,取出劣质、杂质后,将灰树花撕分、清洗后,按照如下方法制备膨化灰树花:Select fresh Grifola frondosa raw materials, remove inferior quality and impurities, tear apart and clean Grifola frondosa, and prepare puffed Grifola frondosa according to the following method:
1、预处理(热烫):同实施例1;1. Pretreatment (hot blanching): same as Example 1;
2、预干燥:把步骤1预处理后的灰树花原料放在热风干燥箱(上海精宏实验设备有限公司,电热恒温鼓风干燥箱:DHG-9123A型)中进行预干燥,干燥温度为70℃,干燥时间5h。按照上述的干燥法测定含水量,结果表明干燥后的灰树花的含水量为21.1%。2, pre-drying: put the Grifola frondosa raw material after the pretreatment of step 1 in the hot-air drying oven (Shanghai Jinghong Experimental Equipment Co., Ltd., electric heating constant temperature blast drying oven: DHG-9123A type) and carry out pre-drying, the drying temperature is 70°C, drying time 5h. Measure water content according to above-mentioned drying method, the result shows that the water content of dried Grifola frondosa is 21.1%.
3、均湿:把步骤2预干燥处理后的灰树花原料进行均湿处理,即将预干燥处理后的原料放在密闭容器中室温贮存24h。3. Humidity equalization: the pre-dried Grifola frondosa raw material in step 2 is subjected to a humidity equalization treatment, that is, the pre-dried raw material is stored in an airtight container at room temperature for 24 hours.
4、变温压差膨化干燥4. Puffing and drying with variable temperature and pressure difference
工艺流程:预干燥的灰树花原料摆盘装入膨化罐、封罐、加温加压、泄压膨化、变温真空脱水、冷却、开罐。Technological process: put the pre-dried Grifola frondosa raw materials into the expansion tank, seal the tank, heat and pressurize, release pressure and expand, change temperature and vacuum dehydration, cool, and open the tank.
具体步骤为:The specific steps are:
1)将步骤3均湿后的灰树花原料放置于膨化罐(QDPH10-1型,天津市勤德新材料科技有限公司)中,加温至温度为90℃,常压保持15分钟;1) Put the Grifola frondosa raw material wetted in step 3 into an expansion tank (QDPH10-1 type, Tianjin Qinde New Material Technology Co., Ltd.), heat it to a temperature of 90°C, and keep it under normal pressure for 15 minutes;
2)再打开真空阀将膨化罐与真空罐连通,抽真空,使压强瞬间降为真空度为-0.098MPa的真空状态;2) Then open the vacuum valve to connect the expansion tank with the vacuum tank, and pump the vacuum to reduce the pressure to a vacuum state with a vacuum degree of -0.098MPa;
关闭真空阀,打开大气阀,使体系恢复常压;Close the vacuum valve, open the atmospheric valve, and restore the system to normal pressure;
再立即打开真空阀,使体系瞬间降为-0.098MPa的真空状态;Then open the vacuum valve immediately, so that the system is instantly reduced to a vacuum state of -0.098MPa;
关闭真空阀,打开大气阀,使体系恢复常压;Close the vacuum valve, open the atmospheric valve, and restore the system to normal pressure;
再立即打开真空阀,使体系瞬间降为-0.0098MPa的真空状态,保持该压强,将温度在1分钟的时间内降至60℃,然后保持146分钟,使灰树花原料含水量为3-5%;Open the vacuum valve immediately again to make the system drop to the vacuum state of -0.0098MPa in an instant, keep the pressure, drop the temperature to 60°C in 1 minute, then keep it for 146 minutes, so that the water content of the Grifola frondosa raw material is 3- 5%;
3)在步骤2)之后,将体系温度先自然冷却至室温再空调冷却至10℃,保持30分钟后破除真空,得到本发明提供的膨化灰树花。3) After step 2), the temperature of the system is naturally cooled to room temperature and then air-conditioned to 10°C, and the vacuum is broken after keeping for 30 minutes to obtain the puffed Grifola frondosa provided by the present invention.
5、分级、包装:将膨化灰树花分级采用手工或自动分级机;包装采用充氮包装。5. Grading and packaging: the puffed maitake frondosa is classified by manual or automatic grading machine; the packaging is packed with nitrogen.
二、膨化灰树花产品的品质检测2. Quality inspection of puffed Grifola frondosa products
1、感官检测1. Sensory testing
将被测样品倒在洁净的白瓷盘中,用肉眼直接观察色泽,形态和杂质,嗅其气味,品尝滋味,结果表明上述制备的膨化灰树花保留了原料的颜色和风味;原料细胞内或细胞间膨胀形成均匀的蜂窝状结构,有很好的酥脆性,口感好,易消化,所得结果如表2所示。Pour the sample to be tested into a clean white porcelain plate, directly observe the color, shape and impurities with the naked eye, smell its smell, taste the taste, the results show that the above prepared puffed Grifola frondosa retains the color and flavor of the raw material; Or the cells expand to form a uniform honeycomb structure, which has good crispness, good taste and easy digestion. The obtained results are shown in Table 2.
表2.感官检测结果Table 2. Sensory test results
2、理化指标的检测2. Detection of physical and chemical indicators
灰树花鲜样含水量为85.82%,含蛋白质31.05%,粗纤维9.76%,可溶性糖40.03%,100g灰树花鲜样粉末提取出灰树花多糖D组分246mg。The fresh sample of Grifola frondosa has a water content of 85.82%, contains 31.05% protein, 9.76% crude fiber, and 40.03% soluble sugar. 246 mg of the Grifola frondosa polysaccharide D component is extracted from 100 g of the fresh sample powder of Grifola frondosa.
按照前述方法检测制备所得膨化灰树花中的蛋白质含量、可溶性糖含量、粗纤维含量、D组分含量,结果表明上述膨化灰树花的含水量为3.23%,蛋白质含量为28.06%,可溶性糖含量为35.37%,粗纤维含量为8.68%,100g膨化粉末提取出灰树花多糖D组分204mg。Detect the protein content, soluble sugar content, crude fiber content, and D component content in the prepared expanded maitake frondosa according to the aforementioned method. The content is 35.37%, the crude fiber content is 8.68%, 100g of puffed powder extracts 204mg of Grifola frondosa polysaccharide D component.
可知,该膨化灰树花能较好地保留并浓缩鲜灰树花中的多种营养成份,如可溶性糖、蛋白质、纤维素等。It can be seen that the expanded Grifola frondosa can better retain and concentrate various nutritional components in fresh Grifola frondosa, such as soluble sugar, protein, cellulose and the like.
3、贮藏期内感官与理化指标检测3. Detection of sensory and physical and chemical indicators during storage
将前述方法检测制备所得包装后的膨化灰树花在常温放置12个月后,检测各项指标,得到其含水量为3.22%,蛋白质含量为28.06%,粗纤维含量为8.67%、可溶性糖含量为35.37%,100g膨化粉末提取出灰树花多糖D组分201mgAfter the packaged puffed frondosa frondosa prepared by the aforementioned method was detected and prepared at room temperature for 12 months, various indicators were detected, and the water content obtained was 3.22%, the protein content was 28.06%, the crude fiber content was 8.67%, and the soluble sugar content 35.37%, 100g puffed powder extracts 201mg of Grifola frondosa polysaccharide D component
结果表明,该膨化灰树花在常温放置12个月后,其色泽、滋味和口感、形状、理化指标与刚制备所得产品相比均无明显变化。The results showed that the color, taste, mouthfeel, shape, and physical and chemical indexes of the puffed frondosa frondosa had no obvious changes compared with those of the freshly prepared product after being placed at room temperature for 12 months.
实施例3、膨化灰树花Embodiment 3, expanded Grifola frondosa
一、膨化灰树花的制备One, the preparation of puffed Grifola frondosa
取新鲜灰树花作为原料,按照实施例1所述方法进行检测,所得检测结果为:灰树花鲜样含水量为84.62%,含蛋白质31.20%,粗纤维9.72%,可溶性糖40.22%,100g灰树花鲜样粉末提取出灰树花多糖D组分249mg。Get fresh Grifola frondosa as raw material, detect according to the method described in Example 1, and the obtained detection result is: Grifola frondosa fresh sample water content is 84.62%, contains protein 31.20%, crude fiber 9.72%, soluble sugar 40.22%, 100g The Grifola frondosa fresh sample powder extracts 249 mg of the Grifola frondosa polysaccharide D component.
选取新鲜灰树花原料,取出劣质、杂质后,将灰树花撕分、清洗后,按照如下方法制备膨化灰树花:Select fresh Grifola frondosa raw materials, remove inferior quality and impurities, tear apart and clean Grifola frondosa, and prepare puffed Grifola frondosa according to the following method:
1、预处理(热烫):同实施例1;1. Pretreatment (hot blanching): same as Example 1;
2、预干燥:把步骤1预处理后的灰树花原料放在热风干燥箱(上海精宏实验设备有限公司,电热恒温鼓风干燥箱:DHG-9123A型)中进行预干燥,干燥温度为90℃,干燥时间3h。按照上述的干燥法测定含水量,结果表明干燥后的灰树花的含水量为20.3%。2, pre-drying: put the Grifola frondosa raw material after the pretreatment of step 1 in the hot-air drying oven (Shanghai Jinghong Experimental Equipment Co., Ltd., electric heating constant temperature blast drying oven: DHG-9123A type) and carry out pre-drying, the drying temperature is 90°C, drying time 3h. Measure water content according to above-mentioned drying method, the result shows that the water content of dried Grifola frondosa is 20.3%.
3、均湿:把步骤2预干燥处理后的灰树花原料进行均湿处理,即将预干燥处理后的原料放在密闭容器中室温贮存24h。3. Humidity equalization: the pre-dried Grifola frondosa raw material in step 2 is subjected to a humidity equalization treatment, that is, the pre-dried raw material is stored in an airtight container at room temperature for 24 hours.
4、变温压差膨化干燥4. Puffing and drying with variable temperature and pressure difference
工艺流程:预干燥的灰树花原料摆盘装入膨化罐、封罐、加温加压、泄压膨化、变温真空脱水、冷却、开罐。Technological process: put the pre-dried Grifola frondosa raw materials into the expansion tank, seal the tank, heat and pressurize, release pressure and expand, change temperature and vacuum dehydration, cool, and open the tank.
具体步骤为:The specific steps are:
1)将步骤3均湿后的灰树花原料放置于膨化罐(QDPH10-1型,天津市勤德新材料科技有限公司)中,加温至温度为120℃,常压保持5分钟;1) Place the Grifola frondosa raw material wetted in step 3 in an expansion tank (QDPH10-1 type, Tianjin Qinde New Material Technology Co., Ltd.), heat to a temperature of 120°C, and keep at normal pressure for 5 minutes;
2)再打开真空阀将膨化罐与真空罐连通,抽真空,使压强瞬间降为真空度为-0.098MPa的真空状态;2) Then open the vacuum valve to connect the expansion tank with the vacuum tank, and pump the vacuum to reduce the pressure to a vacuum state with a vacuum degree of -0.098MPa;
关闭真空阀,打开大气阀,使体系恢复常压;Close the vacuum valve, open the atmospheric valve, and restore the system to normal pressure;
再立即打开真空阀,使体系瞬间降为-0.098MPa的真空状态;Then open the vacuum valve immediately, so that the system is instantly reduced to a vacuum state of -0.098MPa;
关闭真空阀,打开大气阀,使体系恢复常压;Close the vacuum valve, open the atmospheric valve, and restore the system to normal pressure;
再立即打开真空阀,使体系瞬间降为-0.098MPa的真空状态;Then open the vacuum valve immediately, so that the system is instantly reduced to a vacuum state of -0.098MPa;
关闭真空阀,打开大气阀,使体系恢复常压;Close the vacuum valve, open the atmospheric valve, and restore the system to normal pressure;
再立即打开真空阀,使体系瞬间降为-0.098MPa的真空状态;Then open the vacuum valve immediately, so that the system is instantly reduced to a vacuum state of -0.098MPa;
关闭真空阀,打开大气阀,使体系恢复常压;Close the vacuum valve, open the atmospheric valve, and restore the system to normal pressure;
再立即打开真空阀,使体系瞬间降为-0.098MPa的真空状态,保持该压强,将温度在3分钟的时间内降至80℃,然后保持150分钟,使灰树花原料含水量为3-5%;Open the vacuum valve immediately again to make the system drop to a vacuum state of -0.098MPa in an instant, keep the pressure, drop the temperature to 80°C in 3 minutes, and then keep it for 150 minutes, so that the water content of the Grifola frondosa raw material is 3- 5%;
3)在步骤2)之后,将体系温度先自然冷却至室温再空调冷却至20℃,保持50分钟后破除真空,得到本发明提供的膨化灰树花。3) After step 2), the temperature of the system is naturally cooled to room temperature and then air-conditioned to 20°C, kept for 50 minutes and then the vacuum is broken to obtain the puffed Grifola frondosa provided by the present invention.
5、分级、包装:将膨化灰树花分级采用手工或自动分级机;包装采用充氮包装。5. Grading and packaging: the puffed maitake frondosa is classified by manual or automatic grading machine; the packaging is packed with nitrogen.
二、膨化灰树花产品的品质检测2. Quality inspection of puffed Grifola frondosa products
1、感官检测1. Sensory testing
将被测样品倒在洁净的白瓷盘中,用肉眼直接观察色泽,形态和杂质,嗅其气味,品尝滋味,结果表明上述制备的膨化灰树花保留了原料的颜色和风味;原料细胞内或细胞间膨胀形成均匀的蜂窝状结构,有很好的酥脆性,口感好,易消化,所得结果如表3所示。Pour the sample to be tested into a clean white porcelain plate, directly observe the color, shape and impurities with the naked eye, smell its smell, taste the taste, the results show that the above prepared puffed Grifola frondosa retains the color and flavor of the raw material; Or the cells expand to form a uniform honeycomb structure, which has good crispness, good taste and easy digestion. The obtained results are shown in Table 3.
表3.感官检测结果Table 3. Sensory test results
2、理化指标的检测2. Detection of physical and chemical indicators
取新鲜灰树花作为原料,按照实施例1所述方法进行检测,所得检测结果为:灰树花鲜样含水量为84.62%,含蛋白质31.20%,粗纤维9.72%,可溶性糖40.22%,100g灰树花鲜样粉末提取出灰树花多糖D组分249mg。Get fresh Grifola frondosa as raw material, detect according to the method described in Example 1, and the obtained detection result is: Grifola frondosa fresh sample water content is 84.62%, contains protein 31.20%, crude fiber 9.72%, soluble sugar 40.22%, 100g The Grifola frondosa fresh sample powder extracts 249 mg of the Grifola frondosa polysaccharide D component.
按照前述方法检测制备所得膨化灰树花中的蛋白质含量、粗纤维含量、可溶性糖含量、D组分含量,结果表明上述膨化灰树花的含水量为3.15%,蛋白质含量为28.30%,粗纤维含量为8.20%,可溶性糖含量为36.12%,100g膨化灰树花粉末提取出灰树花多糖D组分205mg。Detect the protein content, crude fiber content, soluble sugar content, and D component content in the prepared expanded frondosa frondosa according to the aforementioned method. The content is 8.20%, the soluble sugar content is 36.12%, 100g of puffed grifola frondosa powder extracts 205mg of Grifola frondosa polysaccharide D component.
可知,该膨化灰树花能较好地保留并浓缩鲜灰树花中的多种营养成份,如可溶性糖、蛋白质、纤维素等。It can be seen that the expanded Grifola frondosa can better retain and concentrate various nutritional components in fresh Grifola frondosa, such as soluble sugar, protein, cellulose and the like.
3、贮藏期内感官与理化指标检测3. Detection of sensory and physical and chemical indicators during storage
将前述方法检测制备所得包装后的膨化灰树花在常温放置12个月后,检测各项指标,得到其含水量为3.14%,蛋白质含量为28.28%,粗纤维含量为8.19%,可溶性糖含量为36.08%,100g膨化灰树花粉末提取出灰树花多糖D组分203mg。After the packaged puffed frondosa frondosa prepared by the aforementioned method was detected and prepared at room temperature for 12 months, various indicators were detected, and the water content obtained was 3.14%, the protein content was 28.28%, the crude fiber content was 8.19%, and the soluble sugar content 36.08%, 100g of puffed Grifola frondosa powder extracts 203mg of Grifola frondosa polysaccharide D component.
结果表明,该膨化灰树花在常温放置12个月后,其色泽、滋味和口感、形状、理化指标与刚制备所得产品相比均无明显变化。The results showed that the color, taste, mouthfeel, shape, and physical and chemical indexes of the puffed frondosa frondosa had no obvious changes compared with those of the freshly prepared product after being placed at room temperature for 12 months.
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