CN103512848B - A kind of Rhizoma Coptidis colourity digitlization quality detecting method and equipment thereof - Google Patents

Abstract

The invention provides a digital quality detection method for chroma of Coptidis medicinal material, comprising the following steps: 1) crushing Coptidis medicinal material to obtain Coptidis rhizome powder; Three stimulus values X, Y, Z are determined by colorimetry; 3) Calculate the three stimulus values X, Y, Z to give <i>L ^* , a ^* , b ^* </i>; 4) Pass <i> The position of L ^* , a ^* , b ^* </i> in the coordinate system can be used to determine the variety and quality of Coptis chinensis; each Coptis chinensis variety has a characteristic alkaloid composition, and the alkaloid composition and content reflect the color of Coptis chinensis. Therefore, the chroma of Coptis chinensis can easily distinguish Coptis chinensis species, and can judge the quality of Milian. This method is a new method for the quality and price of Chinese medicinal materials.

Description

A digital chromaticity quality detection method and equipment for Coptis chinensis medicinal material

technical field

The invention relates to a Chinese medicinal material detection device and a detection method thereof, and more specifically relates to a chromaticity digital quality detection method of Coptis chinensis medicinal material and the detection method thereof.

technical background

Coptis chinense is the dried rhizome of Coptischinensis Franch., Coptis deltoidea C.Y. Chenget Hsiao or Coptisteetawall. Coptis deltoidea C.Y. Chenget Hsiao of Ranunculaceae. "Chinese Pharmacopoeia" 2010 edition records the traits, the surface is grayish yellow or yellowish brown, the skin is orange red or dark brown, and the xylem is bright yellow or orange yellow. The content of recorded ingredients is calculated on the basis of dry products, calculated as berberine hydrochloride, containing not less than 5.5% berberine, not less than 0.80% epiberberine, not less than 1.6% coptisine, and not less than palmatine 1.5%. There is also "Jizhu Materia Medica" which records that Jianping produces Coptis chinensis, which is light in color and not used. The color of ancient Chinese herbal medicines is a criterion used for judging.

In modern times, an international consultative body related to color and light—the international standardization group: the International Commission on Illumination (CIE: Commission International de I'Eclairege) was established. CIE formulated the "XYZ (Yxy) Color System" in 1931 and the "L*a*b* Color System (CIE1976Lab Color System)" in 1976. The adoption of these colorimetric systems has made it possible to express uniform chromaticity globally.

At present, the visual method is the most commonly used method, which is low in cost and easy to operate. However, this method only relies on the experimenter's sense to judge the quality of the drug, which is easily affected by the experimenter's subjectivity, so it is not scientific and rigorous; UV-visible spectrophotometry is usually selected in a certain The color of the drug solution is measured at a specific wavelength. In fact, the composition of traditional Chinese medicine preparations is complex, and the color of the drug solution is the result of the joint action of different wavelengths. Therefore, the color of the drug solution cannot be accurately reflected only by the absorbance value of a specific wavelength; the color difference meter measures the color of the drug solution. Relying on the CIELab color space, a specific point in the CIELab three-dimensional coordinates is used to represent a specific color. This method is easy to operate, high in accuracy, and precise in judgment.

Contents of the invention

In order to solve the above problems, the present invention provides a chromaticity digital quality detection device for Coptis rhizome medicinal material. The detection device includes a colorimeter, a light source and a cuvette; wherein the cuvette is placed in a constant temperature device.

The light source is one of standard A light, C light, D65 light, D50 light, ID65 light, ID50 light, F2, F6, F7, F8, F10 light, F11, F12, and the lighting method is diffuse lighting, reflection Light; the observation field of view of the colorimeter: 2° and 10°.

A chromatic digital quality detection method for Coptis rhizome medicinal material, comprising the following steps:

1) Pulverizing Coptidis Rhizoma to obtain Coptidis Rhizoma powder;

2) Put the Coptidis rhizome powder in a cuvette, and measure the three stimulus values X, Y, Z by direct reading method or spectrophotometry;

3) Calculate the tristimulus values X, Y, and Z to give L ^* , a ^* , b ^* ;

4) Judging the variety and quality of Coptis chinensis by the position of L ^* , a ^* , b ^* in the coordinate system.

Step 4) Determine the total alkaloid content of Coptidis Rhizoma by reading the values of L ^* , a ^* and b ^* .

Step 4) By judging the position interval of a ^* and b ^* in the coordinate system, the variety of Coptis chinensis is judged.

The beneficial technical effects of the present invention are: each Coptis chinensis variety has characteristic alkaloid composition, and the alkaloid composition and content reflect the chroma of Coptis chinensis. Therefore, the chroma of Coptis chinensis can easily distinguish Coptis chinensis species, and can judge the quality of Milian. This method is a new method for the quality and price of Chinese medicinal materials. Especially effective for sliced Chinese medicine and powdered Chinese medicine. The method has the advantages of simple operation, shortened time, low cost, feasibility and widespread popularization, and can be used as the basis for the quality and price of Coptis chinensis medicinal materials. Expand more Chinese medicinal materials, and can also apply processing engineering management.

Description of drawings

Figure 1 The position interval of the hue of Milian, Yalian, Yunlian and Coptis chinensis in the two-dimensional coordinate system;

Fig. 2 Relationship between hue (a ^* ) and berberine content in Milian.

detailed description

Example 1 Investigation of Coptis Rhizoma Varieties by Colorimetric Digital Quality Detection Method of Coptis Rhizoma

Purchase the Coptis chinensis medicinal materials of various varieties from the market; specifically as shown in Table 1;

Table 1 Experimental Medicinal Materials

Coptis chinensis bought in the market is first dried at 60°C for more than 8 hours, and then coded (CQMY), and all experimental medicinal materials are stored in Chongqing Academy of Traditional Chinese Medicine.

The reflected light of traditional Chinese medicine powder was measured by chromaticity digital quality detection method of Coptidis rhizome, and the content of six alkaloids in Coptidis rhizome was quantified by HPLC method.

1. Determination of reflected light of traditional Chinese medicine powder by chromaticity digital quality detection method of Coptis chinensis

Conditional diffuse lighting (integrating sphere) mode; reflected light; light source: standard A light, C light, D65 light, D50 light, ID65 light, ID50 light, F2, F6, F7, F8, F10 light, F11, F12; observation field of view : 2° and 10°; color system: CIEL*a*b* color system (CIE); spectral reflectance: 360nm～740nm; special cuvette 12mm*10mm; measurement value: the average after 5 measurements value. Still, the L*a*b* color system is to decompose the light reflected by the object at a wavelength of 360nm~740, and convert it into three numbers according to certain rules. L*a*b*, L*(0.00-100.00) is the lightness, a* and b* the hue.

The measurement condition is 0/d or d/0, D65 light source illumination, 10° field of view, can directly measure the tristimulus X, Y, Z and can directly calculate and give L*, a*, b* and ΔE* and samples The color shade number. In this study, 13 light sources and 2 fields of view were considered for the determination of powder: the difference between 10° and 2° varieties of C light source illumination, the observation condition of 2° field of view is the most obvious, and the average a* value of Milian is 10.31± 1.43, b* value 53.36±6.85, Yalian a* value 8.89, 9.39, b* 33.32, 33.34, Yunlian a* value 5.17, b* value 46.69, and the color of Coptis chinensis is similar to Yunlian, the specific conclusion is shown in Table 2 Show.

Table 2. L*a*b* value of different irradiation light

The hue a* and b* of all medicinal materials are marked in the two-dimensional coordinate system, as shown in Figure 1. Among the three varieties, the a* value of Yunlian was extremely low, and the b* value of Yalian was significantly low. Therefore, the hues a* and b* can distinguish Milian from Yunlian and Yalian.

Example 2 Investigation of Coptis Rhizoma Medicinal Material Chromaticity Digital Quality Detection Method on Coptis Rhizoma Medicinal Material Quality Determination Method

1. Determination of alkaloid content by HPLC method

The average value of 3 copies was determined by HPLC method.

A constant amount of dry powder (passed through a No. 3 sieve) is stirred and mixed, and about 0.5g of the powder of this product is taken.

Preparation of Reference Substance Solution Take appropriate amounts of jatrorrhizine, african tetrandrine, epiberberine, coptisine, palmatine and berberine reference substances, dissolve in methanol to constant volume, and make concentrations of 22.1, 19.5, 65.6, 93.8, 59.4 and 302.8ug/ml mixed reference solution.

Preparation of the test solution Take about 0.2g (3 parts) of the powder of this product, weigh it accurately, put it in a stoppered Erlenmeyer flask, accurately add 50ml of methanol-hydrochloric acid (100:1) mixed solution, seal it tightly, and weigh it. Weight, ultrasonic treatment (power 250W, frequency 40kHz) for 30min, let cool, weigh again, make up for the lost weight with methanol, shake well, filter, accurately measure the filtrate 2ml, put it in a 10ml measuring bottle, add methanol To the mark, shake well, filter, take the continued filtrate, that is.

Instrument LC-20A (Shimadzu, Japan), UV detector. The chromatographic conditions adopt YMC-TriartC ₁₈ chromatographic column (250mm×4.6mm, 5μm); the detection wavelength is 270nm; the mobile phase is acetonitrile A-0.1mol/L ammonium acetate B (gradient elution, see Table 1; 0.1mol/L acetic acid Ammonium was used to adjust the pH value to 9.5 with ammonia water); the flow rate was 1mL·min ^-1 ; the column temperature was 30°C. The number of theoretical plates should not be less than 5×10 ³ , and the elution conditions are shown in Table 3; the jatrorrhizine, africa tetrandrine, epiberberine, coptisine, palmatine and Berberine content.

Table 3 Gradient elution program

2. Combining the L*a*b* values in each Coptidis medicinal material obtained in Example 1 with the content data of Coptidis major alkaloids in each Coptidis medicinal material obtained in this example.

Among the 6 alkaloids in Yunlian and Coptis chinensis than Milian, the contents of tetrandrine, palmatine and epiberberine are extremely low, and the content of jatrorrhizine is relatively high. The specific results are shown in Table 4.

Each alkaloid content in each medical material of table 4

Different varieties of Coptis chinensis have different chroma and alkaloid content. The a* value of Yunlian and Coptis chinensis is low, and the contents of tetrandrine, palmatine and epiberberine are extremely low. There is a correlation between a* value and berberine content in Milian (C light 2° field of view: R ² =0.72, F10 light 10° field of view: R ² =0.75). See Figure 2 for details. The study also found that, in the same place of origin, the same variety, and the same collection time, the first-grade Coptidis rhizome had higher color (L*, a* and b*) and higher total alkaloid content than the second-grade or third-grade varieties. Different grades of medicinal materials are different in size, color, and alkaloid content.

Claims (1)

1. a Rhizoma Coptidis colourity digitlization quality detecting method, is characterized in that: comprise the following steps:

1) Rhizoma Coptidis is pulverized, obtained Golden Thread;

2) Golden Thread is placed in to cuvette, adopts photoelectric tristimulus colorimetry or color measurement by spectrophotometry to determine tristimulus values X, Y, Z;

3) tristimulus values X, Y, Z are calculated and provide L^*,a^*，b^*；

4) pass through L^*,a^*，b^*Kind and the quality of the position judgment Rhizoma Coptidis in coordinate system;

Step 4) is by reading L^*,a^*，b^*Value, judge coptis chinensis total alkaloid content;

Step 4) is by judging a^*，b^*The lane place in coordinate system between, judge the kind of the coptis;

Described checkout equipment comprises colour photometer, light source and cuvette; Wherein cuvette is placed in thermostat; Described light source is markAccurate A light, C light, D65 light, D50 light, ID65 light, ID50 light, F2, F6, F7, F8, F10 light, F11, a kind of light source in F12, shinesBright mode is diffused illumination, reverberation; The field of view angle of described colour photometer is 2 ° and 10 °.