CN1034842A - A method for producing algae with improved biological effect - Google Patents
A method for producing algae with improved biological effect Download PDFInfo
- Publication number
- CN1034842A CN1034842A CN88101399A CN88101399A CN1034842A CN 1034842 A CN1034842 A CN 1034842A CN 88101399 A CN88101399 A CN 88101399A CN 88101399 A CN88101399 A CN 88101399A CN 1034842 A CN1034842 A CN 1034842A
- Authority
- CN
- China
- Prior art keywords
- algae
- selenium
- medium
- chlorella
- scenedesmus
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 241000195493 Cryptophyta Species 0.000 title claims abstract description 116
- 230000004071 biological effect Effects 0.000 title claims abstract description 8
- 238000004519 manufacturing process Methods 0.000 title description 2
- 239000011669 selenium Substances 0.000 claims abstract description 70
- BUGBHKTXTAQXES-UHFFFAOYSA-N Selenium Chemical compound [Se] BUGBHKTXTAQXES-UHFFFAOYSA-N 0.000 claims abstract description 68
- 229910052711 selenium Inorganic materials 0.000 claims abstract description 68
- 238000002360 preparation method Methods 0.000 claims abstract description 6
- 230000000243 photosynthetic effect Effects 0.000 claims abstract description 4
- 229940091258 selenium supplement Drugs 0.000 claims description 66
- 238000000034 method Methods 0.000 claims description 39
- 239000002609 medium Substances 0.000 claims description 30
- 235000015097 nutrients Nutrition 0.000 claims description 19
- 241000195663 Scenedesmus Species 0.000 claims description 11
- 241000195649 Chlorella <Chlorellales> Species 0.000 claims description 10
- BVTBRVFYZUCAKH-UHFFFAOYSA-L disodium selenite Chemical compound [Na+].[Na+].[O-][Se]([O-])=O BVTBRVFYZUCAKH-UHFFFAOYSA-L 0.000 claims description 6
- 239000007788 liquid Substances 0.000 claims description 6
- 239000011781 sodium selenite Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 5
- 239000013505 freshwater Substances 0.000 claims description 5
- 229940065287 selenium compound Drugs 0.000 claims description 5
- 150000003343 selenium compounds Chemical class 0.000 claims description 5
- 229960001471 sodium selenite Drugs 0.000 claims description 5
- 235000015921 sodium selenite Nutrition 0.000 claims description 5
- 235000016425 Arthrospira platensis Nutrition 0.000 claims description 4
- 240000002900 Arthrospira platensis Species 0.000 claims description 4
- IJGRMHOSHXDMSA-UHFFFAOYSA-N Atomic nitrogen Chemical compound N#N IJGRMHOSHXDMSA-UHFFFAOYSA-N 0.000 claims description 4
- CURLTUGMZLYLDI-UHFFFAOYSA-N Carbon dioxide Chemical compound O=C=O CURLTUGMZLYLDI-UHFFFAOYSA-N 0.000 claims description 4
- VZUNGTLZRAYYDE-UHFFFAOYSA-N N-methyl-N'-nitro-N-nitrosoguanidine Chemical compound O=NN(C)C(=N)N[N+]([O-])=O VZUNGTLZRAYYDE-UHFFFAOYSA-N 0.000 claims description 4
- 235000011089 carbon dioxide Nutrition 0.000 claims description 4
- 238000005286 illumination Methods 0.000 claims description 4
- 150000003839 salts Chemical class 0.000 claims description 4
- JPJALAQPGMAKDF-UHFFFAOYSA-N selenium dioxide Chemical compound O=[Se]=O JPJALAQPGMAKDF-UHFFFAOYSA-N 0.000 claims description 4
- 229940082787 spirulina Drugs 0.000 claims description 4
- 240000001131 Nostoc commune Species 0.000 claims description 3
- 235000013817 Nostoc commune Nutrition 0.000 claims description 3
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 claims description 3
- 238000000354 decomposition reaction Methods 0.000 claims description 3
- 229910052760 oxygen Inorganic materials 0.000 claims description 3
- 239000001301 oxygen Substances 0.000 claims description 3
- 241000192698 Aphanocapsa Species 0.000 claims description 2
- OKTJSMMVPCPJKN-UHFFFAOYSA-N Carbon Chemical compound [C] OKTJSMMVPCPJKN-UHFFFAOYSA-N 0.000 claims description 2
- 241000192500 Spirulina sp. Species 0.000 claims description 2
- 241000195647 [Chlorella] fusca Species 0.000 claims description 2
- 229910052799 carbon Inorganic materials 0.000 claims description 2
- 238000000227 grinding Methods 0.000 claims description 2
- 239000001257 hydrogen Substances 0.000 claims description 2
- 229910052739 hydrogen Inorganic materials 0.000 claims description 2
- 238000011081 inoculation Methods 0.000 claims description 2
- 239000012499 inoculation medium Substances 0.000 claims description 2
- 229910052757 nitrogen Inorganic materials 0.000 claims description 2
- 230000003287 optical effect Effects 0.000 claims description 2
- JULROCUWKLNBSN-UHFFFAOYSA-N selenocystine Chemical compound OC(=O)C(N)C[Se][Se]CC(N)C(O)=O JULROCUWKLNBSN-UHFFFAOYSA-N 0.000 claims 2
- RJFAYQIBOAGBLC-BYPYZUCNSA-N Selenium-L-methionine Chemical compound C[Se]CC[C@H](N)C(O)=O RJFAYQIBOAGBLC-BYPYZUCNSA-N 0.000 claims 1
- RJFAYQIBOAGBLC-UHFFFAOYSA-N Selenomethionine Natural products C[Se]CCC(N)C(O)=O RJFAYQIBOAGBLC-UHFFFAOYSA-N 0.000 claims 1
- 125000004435 hydrogen atom Chemical class [H]* 0.000 claims 1
- ZKZBPNGNEQAJSX-UHFFFAOYSA-N selenocysteine Natural products [SeH]CC(N)C(O)=O ZKZBPNGNEQAJSX-UHFFFAOYSA-N 0.000 claims 1
- 229960002718 selenomethionine Drugs 0.000 claims 1
- 238000010348 incorporation Methods 0.000 abstract 1
- 239000000843 powder Substances 0.000 description 20
- 239000000203 mixture Substances 0.000 description 17
- 239000000243 solution Substances 0.000 description 11
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 description 9
- 239000002537 cosmetic Substances 0.000 description 4
- 239000003814 drug Substances 0.000 description 4
- 238000003912 environmental pollution Methods 0.000 description 4
- 235000013305 food Nutrition 0.000 description 4
- 239000000463 material Substances 0.000 description 4
- 238000012545 processing Methods 0.000 description 4
- 210000004027 cell Anatomy 0.000 description 3
- 230000008859 change Effects 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 239000001963 growth medium Substances 0.000 description 3
- 230000008569 process Effects 0.000 description 3
- 238000000746 purification Methods 0.000 description 3
- 239000007787 solid Substances 0.000 description 3
- 206010013786 Dry skin Diseases 0.000 description 2
- 102000004190 Enzymes Human genes 0.000 description 2
- 108090000790 Enzymes Proteins 0.000 description 2
- 102000006587 Glutathione peroxidase Human genes 0.000 description 2
- 108700016172 Glutathione peroxidases Proteins 0.000 description 2
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 description 2
- 238000009395 breeding Methods 0.000 description 2
- 230000001488 breeding effect Effects 0.000 description 2
- 239000012141 concentrate Substances 0.000 description 2
- 239000012153 distilled water Substances 0.000 description 2
- 238000001727 in vivo Methods 0.000 description 2
- 230000000050 nutritive effect Effects 0.000 description 2
- 239000002245 particle Substances 0.000 description 2
- 239000000047 product Substances 0.000 description 2
- VWDWKYIASSYTQR-UHFFFAOYSA-N sodium nitrate Chemical compound [Na+].[O-][N+]([O-])=O VWDWKYIASSYTQR-UHFFFAOYSA-N 0.000 description 2
- 230000001954 sterilising effect Effects 0.000 description 2
- 229920001817 Agar Polymers 0.000 description 1
- RYGMFSIKBFXOCR-UHFFFAOYSA-N Copper Chemical compound [Cu] RYGMFSIKBFXOCR-UHFFFAOYSA-N 0.000 description 1
- 201000000628 Gas Gangrene Diseases 0.000 description 1
- WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 description 1
- UFHFLCQGNIYNRP-UHFFFAOYSA-N Hydrogen Chemical compound [H][H] UFHFLCQGNIYNRP-UHFFFAOYSA-N 0.000 description 1
- 206010020772 Hypertension Diseases 0.000 description 1
- 201000002772 Kwashiorkor Diseases 0.000 description 1
- FFEARJCKVFRZRR-BYPYZUCNSA-N L-methionine Chemical compound CSCC[C@H](N)C(O)=O FFEARJCKVFRZRR-BYPYZUCNSA-N 0.000 description 1
- 241001465754 Metazoa Species 0.000 description 1
- 244000249201 Scenedesmus obliquus Species 0.000 description 1
- 235000007122 Scenedesmus obliquus Nutrition 0.000 description 1
- BQCADISMDOOEFD-UHFFFAOYSA-N Silver Chemical compound [Ag] BQCADISMDOOEFD-UHFFFAOYSA-N 0.000 description 1
- SEQDDYPDSLOBDC-UHFFFAOYSA-N Temazepam Chemical compound N=1C(O)C(=O)N(C)C2=CC=C(Cl)C=C2C=1C1=CC=CC=C1 SEQDDYPDSLOBDC-UHFFFAOYSA-N 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 239000012190 activator Substances 0.000 description 1
- 239000004480 active ingredient Substances 0.000 description 1
- 239000013543 active substance Substances 0.000 description 1
- 239000000654 additive Substances 0.000 description 1
- 230000000996 additive effect Effects 0.000 description 1
- 239000008272 agar Substances 0.000 description 1
- 150000001491 aromatic compounds Chemical class 0.000 description 1
- 230000001580 bacterial effect Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000008901 benefit Effects 0.000 description 1
- 230000008827 biological function Effects 0.000 description 1
- 229910052793 cadmium Inorganic materials 0.000 description 1
- BDOSMKKIYDKNTQ-UHFFFAOYSA-N cadmium atom Chemical compound [Cd] BDOSMKKIYDKNTQ-UHFFFAOYSA-N 0.000 description 1
- 239000002775 capsule Substances 0.000 description 1
- 239000003183 carcinogenic agent Substances 0.000 description 1
- 210000003169 central nervous system Anatomy 0.000 description 1
- 150000001875 compounds Chemical class 0.000 description 1
- 238000001816 cooling Methods 0.000 description 1
- 229910052802 copper Inorganic materials 0.000 description 1
- 239000010949 copper Substances 0.000 description 1
- XUJNEKJLAYXESH-UHFFFAOYSA-N cysteine Natural products SCC(N)C(O)=O XUJNEKJLAYXESH-UHFFFAOYSA-N 0.000 description 1
- 235000018417 cysteine Nutrition 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- ZPWVASYFFYYZEW-UHFFFAOYSA-L dipotassium hydrogen phosphate Chemical compound [K+].[K+].OP([O-])([O-])=O ZPWVASYFFYYZEW-UHFFFAOYSA-L 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 210000003743 erythrocyte Anatomy 0.000 description 1
- 238000001914 filtration Methods 0.000 description 1
- 238000009472 formulation Methods 0.000 description 1
- 239000008103 glucose Substances 0.000 description 1
- 230000036541 health Effects 0.000 description 1
- 229910001385 heavy metal Inorganic materials 0.000 description 1
- 230000006872 improvement Effects 0.000 description 1
- 239000003112 inhibitor Substances 0.000 description 1
- 239000002054 inoculum Substances 0.000 description 1
- 150000002484 inorganic compounds Chemical class 0.000 description 1
- 229910010272 inorganic material Inorganic materials 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 229910000765 intermetallic Inorganic materials 0.000 description 1
- 230000000302 ischemic effect Effects 0.000 description 1
- 229910052943 magnesium sulfate Inorganic materials 0.000 description 1
- 235000019341 magnesium sulphate Nutrition 0.000 description 1
- QSHDDOUJBYECFT-UHFFFAOYSA-N mercury Chemical compound [Hg] QSHDDOUJBYECFT-UHFFFAOYSA-N 0.000 description 1
- 229910052753 mercury Inorganic materials 0.000 description 1
- 229930182817 methionine Natural products 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000010755 mineral Nutrition 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 201000006417 multiple sclerosis Diseases 0.000 description 1
- 206010028320 muscle necrosis Diseases 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000032696 parturition Effects 0.000 description 1
- 201000001245 periodontitis Diseases 0.000 description 1
- 230000001766 physiological effect Effects 0.000 description 1
- 235000020777 polyunsaturated fatty acids Nutrition 0.000 description 1
- 238000012805 post-processing Methods 0.000 description 1
- 235000018102 proteins Nutrition 0.000 description 1
- 108090000623 proteins and genes Proteins 0.000 description 1
- 102000004169 proteins and genes Human genes 0.000 description 1
- 239000013535 sea water Substances 0.000 description 1
- 238000000926 separation method Methods 0.000 description 1
- 229910052709 silver Inorganic materials 0.000 description 1
- 239000004332 silver Substances 0.000 description 1
- 235000010344 sodium nitrate Nutrition 0.000 description 1
- 239000004317 sodium nitrate Substances 0.000 description 1
- 238000004659 sterilization and disinfection Methods 0.000 description 1
- 239000000126 substance Substances 0.000 description 1
- 239000008400 supply water Substances 0.000 description 1
- 230000004083 survival effect Effects 0.000 description 1
- 208000011580 syndromic disease Diseases 0.000 description 1
- 238000007669 thermal treatment Methods 0.000 description 1
- 231100000331 toxic Toxicity 0.000 description 1
- 230000002588 toxic effect Effects 0.000 description 1
- 231100001234 toxic pollutant Toxicity 0.000 description 1
- 231100000419 toxicity Toxicity 0.000 description 1
- 230000001988 toxicity Effects 0.000 description 1
- 239000011573 trace mineral Substances 0.000 description 1
- 235000013619 trace mineral Nutrition 0.000 description 1
- 235000013343 vitamin Nutrition 0.000 description 1
- 239000011782 vitamin Substances 0.000 description 1
- 229940088594 vitamin Drugs 0.000 description 1
- 229930003231 vitamin Natural products 0.000 description 1
- 150000003722 vitamin derivatives Chemical class 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
Classifications
-
- A—HUMAN NECESSITIES
- A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
- A23L—FOODS, FOODSTUFFS OR NON-ALCOHOLIC BEVERAGES, NOT OTHERWISE PROVIDED FOR; PREPARATION OR TREATMENT THEREOF
- A23L17/00—Food-from-the-sea products; Fish products; Fish meal; Fish-egg substitutes; Preparation or treatment thereof
- A23L17/60—Edible seaweed
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N1/00—Microorganisms, e.g. protozoa; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
- C12N1/12—Unicellular algae; Culture media therefor
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Health & Medical Sciences (AREA)
- Biotechnology (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Organic Chemistry (AREA)
- Wood Science & Technology (AREA)
- General Engineering & Computer Science (AREA)
- Tropical Medicine & Parasitology (AREA)
- Cell Biology (AREA)
- Microbiology (AREA)
- Virology (AREA)
- Botany (AREA)
- Biochemistry (AREA)
- Medicinal Chemistry (AREA)
- General Health & Medical Sciences (AREA)
- Biomedical Technology (AREA)
- Marine Sciences & Fisheries (AREA)
- Nutrition Science (AREA)
- Food Science & Technology (AREA)
- Polymers & Plastics (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Cultivation Of Seaweed (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
The invention relates to the preparation of algae rich in selenium and having improved biological effects, under sterile culture conditions, in photosynthetic manner or under air-excluding conditions, to develop algae suitable for incorporation of selenium at a selenium concentration of 10-7-2×10-3Algae were grown in mol/l medium.
Description
The present invention relates to produce the method for the algae of the biological effect that has improved.
From period in time immemorial, the mankind just are used as nutrient and food with algae.The people in the Far East are the main forces of consumption algae; But recently, developed country also uses the algae that does solid form or tablet.Algae is the carrier with high nutritive value, because do the necessary material of healthy life that the algae of solid form contains high concentration, for example, the compound of vitamin, protein, albumen micro element, sugar part, polyunsaturated fatty acids etc.
Recently, the environmental pollution problem that become international.Today, toxic heavy metal (as, lead, mercury, cadmium, copper) and carcinogenic substance (as the polynuclear aromatic compound that concentrates) pollution of ocean be can not be ignored.It is a difficult problem that these materials accumulate in the marine alga body.By ocean " complete marine alga " that obtain or that under other natural endowment, breed, can not be used for human or animal's nutrient, food, cosmetics and medicine; Having only in advance, some components of " the complete marine alga " after biologically harmful substance has been removed in purification are only available.One of this class method of purification is referring to Carames de Guovea " Cosmetics and Toiletries " 95,47(1980).Yet, purification is decomposed a part of biological active ingredients, and therefore, the biological value of the algae component that is obtained reduces (Zajic:Properties and Products of algae:Edition Planum significantly, New York, (1970)).
Therefore, preparation is not subjected to the no Homonemeae of environmental pollution gradually important.
The more known method that artificial culture algae are arranged for example, in the basin under sunlight, or is cultivated algae under natural illumination or artificial lighting or unglazed photograph condition in the enclosure space that guarantees aseptic condition.
According to No. the 5696690th, Japanese publication, marine alga is used in the cultivation of algae, aseptic seawater, and artificial lighting contains the solution of various nutrient salt.
According to No. the 45 17146, Japanese publication, the industrialization of unicellular green chlorella is cultivated and is to use aseptic fresh water medium and finishes under the condition of getting rid of carbonic acid gas and illumination.
The cultivation that French Patent (FRP) also discloses unicellular green algate of fresh water No. 2103462, its industrialized cultivation are to use the nutrient solution that contains suitable nutrient salt to realize in photosynthetic mode.
According to Hungary's publication the 4613/84th and No. 4614/84, the cultivation of algae is in the mineral water at natural origin, medical water or the heat supply water, or in their mixture, and add metallic compound to 10
-2The concentration of mole/L realizes.
In any known method, be used to make all artificial production under aseptic condition of algae of food, feed or medicine.In these methods, the result that condition caused who cultivates algae is that the character of the algae of obtaining is similar to the algae that grows under the natural endowment of non-environmental-pollution; Or because the mode difference of artificial culture makes the character of the algae of obtaining poor slightly.
By the algae of the method artificial culture of prior art, if contain several elements, as selenium, zinc and silver, also trace just.
Be known that selenium has the various biological function, referring to Thressa etal Nutrition Review 35,7(1977), Shamberger.J.of Env.Path.and Tox.4,305(1980), Masukawa et al.Experientia 39,405(1983).What also know is, selenium has the effect that brings high blood pressure down, and improves the ischaemic of heart, the state of the low and infraction of oxygen content, and the wax lipofuscinization (Ceroidal lipofuscinosis) that suppresses central nervous system; Selenium also has the effect of improvement to periodontitis, reduces cancered possibility; In addition, selenium also is considered to a kind of sudden change inhibitor.Also certifiedly be, scarce selenium can cause multiple disease, for example, hepatonecrosis, myonecrosis, the erythrocyte film rupture, the ST in the electrocardiogram raises, kwashiorkor syndrome and multiple sclerosis.
The beneficial effect of selenium mainly is based on its effect that activates glutathione peroxidase, and this enzyme is that important interior the giving birth to of suppressing harmful peroxidating process suppressed son.As the indispensable composition of gst enzyme prothetic group, selenium is one of most important indispensable material in the life, and selenium does not gather in vivo, therefore will constantly replenish.Until today, also can only pass through inorganic compound (selenium dioxide, sodium selenite etc.) selenium is introduced in the organism.
The objective of the invention is to provide the algae of the special biological property of tool, and, because increasing of Se content can guarantee the biological effect of selenium.
The present invention is based on such understanding, promptly under certain condition, algae can not only survive (just not dying) in containing the solution of selenium, and can also grow, thereby, can in the nutritive solution that contains high concentration selenium, cultivate.This is wonderful understanding, because prior art thinks that algae can be killed by the selenium of toxicity.Moreover the present invention also is based on the recognition, that is, selenium is incorporated in containing under a certain condition in the algae bio body of cultivating in the seleno culture medium.
Therefore, the present invention relates to prepare the method for the algae of the biological effect that tool improved, wherein, the selected algae that goes out is under aseptic condition, in the medium that contains fresh water and nutrient salt, under the situation that has carbonic acid gas and light to exist, cultivate in photosynthetic mode, perhaps, under no optical condition, also containing carbon, nitrogen, hydrogen is cultivated in the medium of oxygen, then, separate the algae of being obtained.According to the present invention, inorganic or (with) organically selenium compound is with 10
-7To 2 * 10
-3The concentration of mol is added in the medium, and then, inoculation can be mixed the pure culture of the algae of selenium on medium.The acquisition methods of this pure culture is, selected algae is inoculated in the liquid nutrient medium, and this medium contains inorganic (also can contain organically) nutrient component, handles with N-methyl-N '-nitro-N-nitrosoguanidine then.In the single algae of being obtained by sudden change, select in the time of in containing seleno culture medium, cultivating and can mix selenium, and identical with the wild strain at least algae of growth rate.Behind the pure culture inoculation medium that obtains like this, through the nurturing period, isolate algae, be dried to optimum moisture content if desired, again, make it to decompose with known ultrasonic method or Ginding process.
According to the present invention, the pure culture that can mix the algae of selenium preferably prepares from monoplast green alga or blue algae, as chlorella (Chlorellasp), grid algae (Scenedesmus sp.) and spirulina (Spirulina sp.), concrete grammar are, after handling with N-methyl-N '-nitro-N-nitrosoguanidine, carefully cell is come out, be distributed in a series of solid culture mediums, these medium are made through dilution, and to have replenished concentration be 10
-7To 2 * 10
-3The selenium compound of mol.In the algae of being turned out, select ramp and the most preferred strain that can mix selenium again, further cultivate with liquid nutrient medium, the pure algae culture that obtains like this be can be used for industrial.
Like this, at growing period, selenium is incorporated in the algae bio body by above-mentioned processing, and the original algae of its concentration average specific exceeds 10
4Doubly.
Cultivate in the medium of the algae can mix selenium in industrialization, with distilled water as liquid.In this class medium, except the nutriment of routine, also replenish with inorganic or (with) organic selenium compound.Inoculum so preparation and the culture medium inoculated algae through sterilizing, the promptly aforesaid kind that can mix selenium.In its process of growth, algae is taken in selenium in its organism, and does not have the above-described toxic pollutant of not expecting.
The algae of so preparation is isolated from medium, use known method, under temperate condition,, the highlyest be no more than 80 ℃, carry out drying with 65 ℃ of the suitableeest temperature.Dried algae is with grinding the particle that suitably is decomposed into about 1 micron size.In addition, wet algae concentrate also can decompose through ultrasonic processing, then with above-mentioned condition drying.
What obtain like this is that selenium content is the algae powder of 250-400 μ g/g, it can directly be employed or consume, or be used for food, in feed and the cosmetics, or use with medicine and biological active agents, or (with) as the additive of these materials, its form can be any commercial form of these products, preferably tablet, capsule or other formulations.
The major advantage of the inventive method can be summarized as follows:
A) algae can also be cultivated with easy equipment by the method that cheapness is easily gone.
B) do not need purifying and the rapid post processing of multistep after the cultivation.
C) algae is cultivated down in aseptic condition, has got rid of environmental pollution, thereby, there is no doubt that the algae of such acquisition can be by the human consumption.
D) it is higher and have the biology that improved and an algae of physiological effect to access the selenium content that has than existing algae.
E) according to algae that the inventive method obtained widely (and preferably) be applied to nutriment, cosmetics, medicine and other field.
The most preferred application of algae that obtains according to the inventive method is the treatment field, because selenium is the activator of glutathione peroxidase prothetic group.Selenium is not assembled in vivo, uses the algae that the inventive method obtained then can Selenium Supplement, thereby makes owing to lack the caused various Health cost of selenium and all can obtain medical treatment.
Following non-limiting instance is understood the present invention in more detail.
Example 1
At 25-27 ℃, grid algae (Scenedesmus obtisiusculus) is seeded on the BoldShi liquid nutrient medium that contains 100-500 μ g/ml N-methyl-N '-nitro-N-nitrosoguanidine, be placed in the 250ml flask shake and cultivated 30 minutes.Then, water carefully washes out cell, is distributed on a series of BoldShi medium that solidify with agar.Contain selenium in this a series of medium, its concentration is since 3.125 μ g/ml, by increasing exponentially until 400 μ g/ml.The colony of growth and survival is separated on these medium, and selected cell-line is bred with laboratory-scale at least in the BoldShi nutrient solution that contains 20 μ g/ml selenium.Be suitable for most mixing selenium and colony that have the growth rate of almost leading mutually with wild (contrast) strain breeds at least in the medium that contains 20 μ g/ml selenium.
Cell separation is gone out, wash with water carefully, ultrasonic decomposition is with 65 ℃ of dryings, again with the selenium content of aas determination algae.
As follows by the above-mentioned selenium content that obtains the algae powder:
The kind selenium content
(μ g/g algae powder)
Untreated wild I numbers 50
Untreated wild II numbers 30
FM-I-120 1300
FM-I-1871 2400
FM-441/87 1600
FM-449/87 1800
Embodiment 2
The 40mg sodium selenite be added to be loaded on 10 liter capacities the algae reproduction vial in 8 liters of Knop-PringsheimShi medium on.The nutrient solution that is obtained was sterilized 30 minutes in 121 ℃ under the pressure that surpasses a crust.With the sterile liquid cooling, inoculate with the pure culture of the grid algae (Scenedesmus obtisiusculus) that can mix selenium again.At 25 ℃, the filtrated air that will contain 5% carbonic acid gas is blown into medium, and whole system is thrown light on discharge tube, and illumination is 4000lux, and wavelength is 440-520 and 640-700 μ m.
After cultivating in 14 days, from medium, isolate algae, wash with water, ultrasonic processing is decomposed, careful dry below 65 ℃.The selenium content of the algae powder that obtains is 1200 μ g/g.
Embodiment 3
Be loaded in 8 liters of distilled water in 10 liters of algae reproduction devices, dissolve in 8.0g sodium nitrate, 0.8g magnesium sulfate semihydrate, 0.8g dipotassium hydrogen phosphate, 2.5ml AronShi trace element solution, 5g glucose, 0.1g cysteine, 0.1g methionine.In this nutrient solution, replenish the 50mg sodium selenite, filter, guaranteeing to inoculate the grid algae (Scenedesmus obtisiusculus) of pure mixed selenium under the aseptic condition with sterile filters.Through 4 days after the breeding of 25-28 ℃ of dark place, from medium, tell algae, wash with water, ultrasonic decomposition, dry below 65 ℃.The Se content of the algae powder that obtains is 1380 μ g/g.
Embodiment 4
Follow embodiment 2 described methods,, replace G-5 type sterilization bacterial filter filter-sterilized medium just without thermal treatment.The selenium content of the algae powder that obtains is 1300 μ g/g.
Embodiment 5
Press embodiment 2 or 3 described methods, difference is, replaces grid algaes (Scenedesmus obtisiusculus) with the chlorella that can mix selenium (Chloella vulgaris) that obtains through embodiment 1 described sudden change processing.
The selenium content of the algae powder that obtains is:
The kind selenium content
(μ g/g algae powder)
" wild " I type 140
" wild " II type 140
DV-35-42 3200
DV-78-20 2300
DV-104-21 1500
Embodiment 6
Follow embodiment 2 or 3 described methods, just replace grid algaes (Scenedesmus obtisiusculus) with the chlorella (Chlorella minitissima) that can mix selenium.The selenium content of the algae powder that obtains is 1400 μ g/g.
Embodiment 7
Follow embodiment 2 or 3 described methods, difference is, heat up 50 ℃ of as many as of nutrient solution, the algae (Aphanocapsa thermalis) of using heatproof and can mix selenium).The selenium content of the algae powder that obtains is:
The kind selenium content
(μ g/g algae powder)
" wild " type 130
DV-12-220 1100
DV-12-340 1550
Embodiment 8
Follow embodiment 2 described methods, difference is, uses to belong to blue algae and spirulina that can mix selenium (Spirulian sp.) replacement grid algaes (Scenedesmus odtisiusculus).The selenium content of the algae powder that obtains is:
The kind selenium content
(μ g/g algae powder)
" wild " type 130
DV-12-220 1100
DV-12-340 1550
Embodiment 8
Follow embodiment 2 described methods, difference is, uses to belong to blue algae and spirulina that can mix selenium (Spirulian sp) replacement grid algaes (Scenedesmus obtisiusculus).The selenium content of the algae powder that obtains is:
The kind selenium content
(μ g/g algae powder)
" wild " I type 50
" wild " II type 30
HE-87-104 1100
HE-89-241 1200
HE-89-302 1500
Embodiment 9
Follow embodiment 2 described methods, difference is that the temperature of nutrient solution is reduced to 8 ℃, uses thread blue algae (Nostoc commune) cold-resistant and that can mix selenium.The selenium content of the algae powder that obtains is:
The kind selenium content
(μ g/g algae powder)
" wild " type 140
BK-1218-2 1140
Embodiment 10
Through sterilised filtration and be loaded in 8 liters of BoldShi medium in 10 liters of algae reproduction bottles and add 5 * 10
-3Sodium selenite.Inoculate the medium that is obtained with the chlorella that can mix selenium (Chlorella fusca) by embodiment 1 described preparation.Made algae propagation 14 days by embodiment 2 is described.Filter then, carefully wash with water, dry below 80 ℃.The selenium content of the algae powder that obtains is 2700 μ g/g.
Embodiment 11
Be inoculated into the grid algae that can mix selenium (Scenedesmus obliquus) and contain 8 liters of BoldShi nutrient solutions and 10 by embodiment 1 described breeding
-7In the blake bottle of M sodium selenite, then, press embodiment 10 described methods, the selenium content of the algae powder that obtains is 300 μ g/g.
Embodiment 12
Follow the method for embodiment 2, difference is that isolated algae is ground and decomposes to 1 μ m particle 65 ℃ of dryings, and the selenium content of the algae powder that obtains is 1200 μ g/g.
Claims (10)
1, a kind ofly prepares the method that tool improves the algae of biological effect, wherein, under aseptic condition, having under carbonic acid gas existence and the illumination condition, in the medium that contains fresh water and nutrient salt, cultivate selected algae in photosynthetic mode, perhaps under no optical condition, cultivate selected algae in the medium that also contains carbon, hydrogen, oxygen and nitrogen, this method comprises, with 10
-7-2 * 10
-3The concentration of mol add inorganic or (with) organic selenium compound, can mix the pure algae kind inoculation medium of selenium, if desired, in cultivation with after with ultrasonic or grinding decomposition algae, make its drying, wherein pure algae kind preparation method is the liquid nutrient medium that contains inorganic (also can contain organically) nutrient with the selected algae inoculation that goes out, handle with N-methyl-N '-nitro-N-nitrosoguanidine again, then, in the medium that also contains selenium in addition, cultivate this kind algae, at last, select and to mix the algae that selenium simultaneously has the growth rate that equates with original algae at least again.
2, method according to claim 1, comprising in medium, use sodium selenite and (or) selenium dioxide is as inorganic selenium.
3, method according to claim 1, comprising in medium, use selenocystine and (or) selenomethionine is as organic selenium compounds.
4, method according to claim 1, comprising the kind of using chlorella (Chlorella) or grid algae (Scenedesmus) as the selected algae that goes out.
5, method according to claim 1 is comprising using algae (Aphanocapsa thermalis) as selected algae.
6, method according to claim 1 is comprising using Nostoc commune (Nostoc commune) as the algae of selecting.
7, method according to claim 1 is comprising using chlorella (Chlorella minitissim or Chlorella fusca) as the algae of selecting.
8, method according to claim 1 is comprising using the blue algae of fresh water as the algae of selecting.
9, method according to claim 8 is comprising using spirulina (Spirulina sp.) as blue algae.
10, method according to claim 1 is comprising using grid algaes (Scenedesmus obtisiusculus or Scenedesmus oblignus) as the selected algae that goes out.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| HU575/88 | 1988-02-09 | ||
| HU88575A HU202272B (en) | 1988-02-09 | 1988-02-09 | Process for producing algae |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN1034842A true CN1034842A (en) | 1989-08-23 |
Family
ID=10950225
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN88101399A Pending CN1034842A (en) | 1988-02-09 | 1988-03-18 | A method for producing algae with improved biological effect |
Country Status (26)
| Country | Link |
|---|---|
| JP (1) | JPH01215278A (en) |
| KR (1) | KR890014024A (en) |
| CN (1) | CN1034842A (en) |
| AT (1) | AT389888B (en) |
| AU (1) | AU606138B2 (en) |
| BE (1) | BE1001675A3 (en) |
| CA (1) | CA1310927C (en) |
| CH (1) | CH676605A5 (en) |
| DD (1) | DD267994A5 (en) |
| DK (1) | DK148888A (en) |
| ES (1) | ES2006114A6 (en) |
| FI (1) | FI881310A (en) |
| FR (1) | FR2626890B1 (en) |
| GB (1) | GB2214928B (en) |
| GR (1) | GR1000446B (en) |
| HU (1) | HU202272B (en) |
| IL (1) | IL85777A (en) |
| IT (1) | IT1216140B (en) |
| LU (1) | LU87172A1 (en) |
| NL (1) | NL8800692A (en) |
| NO (1) | NO881215L (en) |
| PL (1) | PL271284A1 (en) |
| PT (1) | PT87016B (en) |
| SE (1) | SE468815B (en) |
| YU (1) | YU55488A (en) |
| ZA (1) | ZA881956B (en) |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102307486A (en) * | 2009-02-04 | 2012-01-04 | 小球藻工业株式会社 | Selenium-containing unicellular microalgae for animal plankton feeds and method of culturing selenium-containing animal planktons using the same |
| CN110317769A (en) * | 2019-07-18 | 2019-10-11 | 恩施硒圣植物科技有限公司 | A kind of cultural method of selenium-rich microalgae |
| CN115786128A (en) * | 2022-12-15 | 2023-03-14 | 宁波浮田生物技术有限公司 | Linear round sifting algae culture medium and culture method |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US5211003A (en) * | 1992-02-05 | 1993-05-18 | General Electric Company | Diffuser clean air bleed assembly |
| EP1731597A1 (en) * | 2005-06-06 | 2006-12-13 | Johannes Gutenberg-Universität Mainz | Selenium-enriched liquid media for the cultivation of siliceous sponges and for biogenic silica production |
| CZ300809B6 (en) * | 2007-11-02 | 2009-08-12 | Mikrobiologický ústav AV CR, v.v.i. | Industrial strain Scenedesmus quadricauda SeVI of green chlorococcale alga Scenedesmus quadricauda (Turpin) Brebisson |
| CZ300808B6 (en) * | 2007-11-02 | 2009-08-12 | Mikrobiologický ústav AV CR, v.v.i. | Industrial strain Scenedesmus quadricauda SeIV+VI of green chlorococcale alga Scenedesmus quadricauda (Turpin) Brebisson |
| CZ300861B6 (en) * | 2007-11-02 | 2009-08-26 | Mikrobiologický ústav AV CR, v.v.i. | Industrial strain SeIV of green chlorococcale alga Scenedesmus quadricauda (Turpin) Brebisson |
| FR2935394B1 (en) * | 2008-08-29 | 2013-03-08 | Eco Solution | ORGANIC SELENIUM-ENRICHED PHOTOSYNTHETIC MICROORGANISMS FROM SELENO-HYDROXYACID COMPOUNDS AND THEIR USE IN NUTRITION, COSMETICS AND PHARMACY |
| TWI495725B (en) * | 2009-08-28 | 2015-08-11 | Metabolium | Photosynthetic microorganisms enriched in selenium using selenohydroxy acid compounds, uses thereof in nutrition, cosmetics and pharmacy |
| MD4395C1 (en) * | 2014-11-28 | 2016-08-31 | Институт Зоологии Академии Наук Молдовы | Strain of green microalga Scenedesmus quadricauda var. quadricauda - source of proteins, glucides and lipids |
| WO2018199723A2 (en) * | 2017-04-28 | 2018-11-01 | 한남바이오 주식회사 | Selenium resistive novel microalgae |
| WO2022009215A1 (en) * | 2020-07-08 | 2022-01-13 | Council Of Scientific And Industrial Research (An Indian Registered Body Incorporated Under The Regn. Of Soc. Act (Act Xxi Of 1860)) | Organic selenium enriched edible marine microalgal biomass |
Family Cites Families (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| DD246789A (en) * | ||||
| FR2426404A1 (en) * | 1978-05-26 | 1979-12-21 | Brevier Jean | Culture of phytoplankton in sea water - enriched with minerals, vitamin(s) and aminoacid(s), used in cosmetics and in human and veterinary medicine |
| DE3421644A1 (en) * | 1984-06-09 | 1985-12-12 | Richard 7880 Bad Säckingen Hau | DIET DIAGRAM |
| JPH062047B2 (en) * | 1985-10-11 | 1994-01-12 | 有限会社サン・ファーム | Spirulina containing high concentration of trace elements in human body and production method thereof |
| EP0239272B1 (en) * | 1986-03-19 | 1993-03-03 | Biotechna Limited | Improvements relating to biomass production |
-
1988
- 1988-02-09 HU HU88575A patent/HU202272B/en not_active IP Right Cessation
- 1988-03-17 CH CH1010/88A patent/CH676605A5/de not_active IP Right Cessation
- 1988-03-18 KR KR1019880002909A patent/KR890014024A/en not_active Application Discontinuation
- 1988-03-18 ZA ZA881956A patent/ZA881956B/en unknown
- 1988-03-18 AT AT0073588A patent/AT389888B/en not_active IP Right Cessation
- 1988-03-18 JP JP63063751A patent/JPH01215278A/en active Pending
- 1988-03-18 SE SE8800991A patent/SE468815B/en not_active IP Right Cessation
- 1988-03-18 BE BE8800310A patent/BE1001675A3/en not_active IP Right Cessation
- 1988-03-18 DD DD31381788A patent/DD267994A5/en unknown
- 1988-03-18 GR GR880100171A patent/GR1000446B/en unknown
- 1988-03-18 CA CA000561888A patent/CA1310927C/en not_active Expired - Fee Related
- 1988-03-18 DK DK148888A patent/DK148888A/en not_active Application Discontinuation
- 1988-03-18 AU AU13262/88A patent/AU606138B2/en not_active Ceased
- 1988-03-18 IL IL85777A patent/IL85777A/en unknown
- 1988-03-18 ES ES8800828A patent/ES2006114A6/en not_active Expired
- 1988-03-18 NO NO88881215A patent/NO881215L/en unknown
- 1988-03-18 PL PL27128488A patent/PL271284A1/en unknown
- 1988-03-18 FI FI881310A patent/FI881310A/en not_active Application Discontinuation
- 1988-03-18 YU YU00554/88A patent/YU55488A/en unknown
- 1988-03-18 IT IT8819836A patent/IT1216140B/en active
- 1988-03-18 PT PT87016A patent/PT87016B/en not_active IP Right Cessation
- 1988-03-18 CN CN88101399A patent/CN1034842A/en active Pending
- 1988-03-18 LU LU87172A patent/LU87172A1/en unknown
- 1988-03-18 NL NL8800692A patent/NL8800692A/en not_active Application Discontinuation
- 1988-03-18 GB GB8806532A patent/GB2214928B/en not_active Expired - Fee Related
- 1988-03-18 FR FR888803516A patent/FR2626890B1/en not_active Expired - Fee Related
Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN102307486A (en) * | 2009-02-04 | 2012-01-04 | 小球藻工业株式会社 | Selenium-containing unicellular microalgae for animal plankton feeds and method of culturing selenium-containing animal planktons using the same |
| CN110317769A (en) * | 2019-07-18 | 2019-10-11 | 恩施硒圣植物科技有限公司 | A kind of cultural method of selenium-rich microalgae |
| CN110317769B (en) * | 2019-07-18 | 2021-04-20 | 恩施硒圣植物科技有限公司 | Culture method of selenium-rich microalgae |
| CN115786128A (en) * | 2022-12-15 | 2023-03-14 | 宁波浮田生物技术有限公司 | Linear round sifting algae culture medium and culture method |
Also Published As
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Osinga et al. | Cultivation of marine sponges | |
| CN1500749A (en) | Modifying agent for microorganism breeding water and preparing method thereof | |
| CN1034842A (en) | A method for producing algae with improved biological effect | |
| CN102925359A (en) | Seawater spirulina selenium-rich culture method | |
| Zmora et al. | 20 Microalgae for Aquaculture | |
| WO2010089864A1 (en) | Selenium-containing unicellular microalgae for animal plankton feeds and method of culturing selenium-containing animal planktons using the same | |
| CN109618997A (en) | The ecological purification method of oyster culture | |
| CN101078004A (en) | Microorganism preparation for modifying water body by using bacterial and manufacturing method thereof | |
| CN1330711A (en) | Chlorophyll-rich and salt-resistant chlorella | |
| Lezama-Cervantes et al. | Effects of constructed microbial mats on water quality and performance of Litopenaeus vannamei post-larvae | |
| CN1760146A (en) | Nourishing water transfer agent for aquiculture | |
| KR101822736B1 (en) | Feed stuff for sea cucumber including organic compounds in biofloc and method for preparation thereof | |
| CN1498865A (en) | Micro ecological agent for supporting water in fishing use and its prepn. method | |
| CN1313395C (en) | Super-concentrated algae bacterium micro-ecological balance suspension type water quality modifier and preparation method thereof | |
| CN1600847A (en) | Artificial cultivated ganoderma lucidum rich in germanium and method of growth in liquor | |
| Chen et al. | A preliminary study on the potential value of a novel integrated aquaculture system on water purification | |
| RU2787046C1 (en) | Method for feeding the larvae and juvenile specimens of strongylocentrotus intermedius | |
| CN1092104A (en) | The preparation method of germanium and selenium spirulina | |
| KR20200121524A (en) | Method for Increasing of Fucoxanthin as Sub-pigment in a Diatom | |
| RU2793471C1 (en) | Method for cultivation of chaetoceros muelleri and isochrysis galbana unicellular microalgae - live food for larvae of marine invertebrates | |
| Rajendran et al. | Spirulina spp. isolation and cultivation for fish feed | |
| KR101795174B1 (en) | culturing method for aquatic plant using δ-aminolevulinic acid | |
| CN118743381A (en) | A method for cultivating green clam larvae | |
| Amalia et al. | Low Temperature Preservation on Dense Biomass of Nannochloropsis oculata | |
| JP2007006763A (en) | Aquatic animal feed and method for producing the same |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C01 | Deemed withdrawal of patent application (patent law 1993) | ||
| WD01 | Invention patent application deemed withdrawn after publication |