CN103478876B - Solid ecological cultivation and drying machine - Google Patents
Solid ecological cultivation and drying machine Download PDFInfo
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Abstract
本发明属于生物发酵技术领域,具体涉及一种固体生态培养、干燥一体机。该一体机由热风发生系统、液体输入装置、旋转物料处理系统、机架装置、旋风分离器以及功能控制系统构成。本发明从仿生学角度研制相关结构,能够自动化控制有氧生物发酵和温敏干燥过程的相关环节,提供良好通风和适宜温、湿度条件,确保益生菌群的高效培育,并能有效地达到较好的封闭性能,使杂菌率控制在1%以下。适用于好氧益生菌类固体培养与温敏干燥一体化的工厂规模化生产。
The invention belongs to the technical field of biological fermentation, and in particular relates to a solid ecological cultivation and drying integrated machine. The all-in-one machine is composed of a hot air generating system, a liquid input device, a rotating material handling system, a rack device, a cyclone separator and a functional control system. The present invention develops related structures from the perspective of bionics, can automatically control the relevant links of aerobic biological fermentation and temperature-sensitive drying process, provide good ventilation and suitable temperature and humidity conditions, ensure the efficient cultivation of probiotics, and can effectively achieve higher Good sealing performance, so that the bacteria rate is controlled below 1%. It is suitable for the factory-scale production of the integration of aerobic probiotic solid culture and temperature-sensitive drying.
Description
技术领域technical field
本发明属于生物发酵技术领域,具体涉及有氧生物发酵的工厂规模化生产,如动物微生物饲料添加剂生产,医药生物制药、食品发酵生产,以及有氧菌群固体发酵培养生产的相关领域。The invention belongs to the technical field of biological fermentation, and specifically relates to the factory-scale production of aerobic biological fermentation, such as the production of animal microbial feed additives, pharmaceutical biopharmaceuticals, food fermentation production, and related fields of solid fermentation culture production of aerobic flora.
背景技术Background technique
益生菌可以制成各种功能性微生物制剂。益生菌在代谢过程中生成的菌质极富重要的生物活性物质。作为一种新型的动物微生物饲料添加剂,具有无毒副作用,能促进动物生长,提高饲料转化比,改善动物消化道微生态环境,增强动物免疫力等优点。如何从仿生学角度以最优化条件生态型、高效率固体培育好氧性益生菌群,并能在较低温条件下快速干燥益生菌培养物料等方面面临困难,已成为生物发酵领域工厂规模化生产的瓶颈,备受关注。尤其是抗生素滥用的今天,积极寻找抗生素替代品,规模化发展绿色养殖业是关系到国计民生的重要研究工作。目前有关益生菌固体培养的工厂化生产方式,还是局限在静态培养,人工搅拌,表现为供氧不充分,堆积物料内部温度偏高,温度不均匀,湿度不稳定,存在严重的厌氧发酵等情况,导致好氧性益生菌活菌数不高,活性较差。虽然在终产品中可能益生菌芽孢数能达到一定水平,但产量不高。此外,人工翻拌培养物料会导致细菌芽孢被动性吸入操作人员呼吸系统,引起条件致病。相伴随的问题是有氧生物发酵完成后的培养物料如何能够在较低温条件下(≤50℃)快速干燥,也是当前益生菌固体发酵工厂化生产以及各类生物制药、食品发酵领域的工厂化生产面临的一大难题。Probiotics can be made into various functional microbial preparations. The bacteria produced by probiotics during their metabolism are rich in important biologically active substances. As a new type of animal microbial feed additive, it has no toxic side effects, can promote animal growth, increase feed conversion ratio, improve animal digestive tract micro-ecological environment, and enhance animal immunity. From the perspective of bionics, how to cultivate aerobic probiotics with optimized conditions and high-efficiency solids, and how to quickly dry probiotic culture materials at low temperatures has become a large-scale factory production in the field of biological fermentation. The bottleneck has attracted much attention. Especially in today's overuse of antibiotics, it is an important research work related to the national economy and the people's livelihood to actively seek alternatives to antibiotics and develop green farming on a large scale. At present, the industrial production method of solid culture of probiotics is still limited to static culture and manual stirring, which is manifested by insufficient oxygen supply, high internal temperature of the piled material, uneven temperature, unstable humidity, and severe anaerobic fermentation, etc. In this case, the number of live bacteria of aerobic probiotics is not high, and the activity is poor. Although the number of probiotic spores may reach a certain level in the final product, the yield is not high. In addition, manual mixing of culture materials will lead to passive inhalation of bacterial spores into the respiratory system of operators, causing conditional disease. The accompanying problem is how to quickly dry the culture material after the completion of aerobic biological fermentation at a lower temperature (≤50°C), which is also the current industrialized production of probiotic solid fermentation and the industrialization of various biopharmaceuticals and food fermentation fields. A big problem facing production.
本发明能够遵循自然界好氧性益生菌生长繁殖所需的生长代谢规律,从仿生学角度提供优化的适合好氧性益生菌群生长繁育的相关条件,高效率有氧生物发酵益生菌群,并在短时间内,以培养-干燥一体化方式富氧发酵和快速温敏干燥发酵物料,极大限度地保护益生菌群活性,最大限度的减少杂菌率,可为工厂规模化生产好氧益生菌产品提供新型高效的固体生态培养、干燥一体机。The present invention can follow the growth and metabolism rules required for the growth and reproduction of aerobic probiotics in nature, provide optimized relevant conditions suitable for the growth and breeding of aerobic probiotics from the perspective of bionics, high-efficiency aerobic biological fermentation of probiotics, and In a short period of time, aerobic fermentation and rapid temperature-sensitive drying of fermented materials are carried out in a culture-drying integrated way, which can protect the activity of probiotics to the greatest extent, reduce the rate of miscellaneous bacteria to the greatest extent, and can produce aerobic probiotics on a large scale in the factory Bacteria products provide a new type of high-efficiency solid ecological cultivation and drying machine.
目前功能性微生态饲料添加剂的研究与应用是畜牧业生产发展中的热点之一,有益菌群及其代谢产物具有极高的机体代谢调节功能和营养作用,是缓解抗生素滥用现状的有效手段,是国家相关职能部门当前极力推广的科技应用项目之一,各类生物制药、食品生产领域的工厂化生产设备技术也均需改造换代。At present, the research and application of functional microecological feed additives is one of the hotspots in the development of animal husbandry production. Beneficial bacteria and their metabolites have extremely high functions of body metabolism regulation and nutrition, and are an effective means to alleviate the current situation of antibiotic abuse. It is one of the science and technology application projects that the relevant functional departments of the state are currently vigorously promoting. The factory production equipment technology in the fields of various biopharmaceuticals and food production also needs to be upgraded.
由于本发明作为益生菌等生物发酵的主体关键设备,能够直接利用农副产品原料进行工厂规模化富氧生物发酵生产,而且采用固体发酵比目前的液体发酵而言,能使功能性动物微生物饲料添加剂等产品的生产效率、产品质量极大提高,生产成本大幅度降低,市场应用前景广阔,其经济效益的提高将极其显著。As the main key equipment of biological fermentation such as probiotics, the present invention can directly use the raw materials of agricultural and sideline products to carry out large-scale oxygen-enriched biological fermentation production, and the use of solid fermentation can make functional animal microbial feed additives more efficient than the current liquid fermentation. The production efficiency and product quality of such products are greatly improved, the production cost is greatly reduced, the market application prospect is broad, and the improvement of its economic benefits will be extremely significant.
发明内容Contents of the invention
本发明的目的是提供一种能够在较低温条件下,以培养-干燥一体化方式生态仿生型培养好氧益生菌群以及快速温敏干燥(≤50℃)培养物料,极大限度地保护益生菌群活性,最大限度的减少杂菌率的固体生态培养、干燥一体机,技术方案如下:The purpose of the present invention is to provide an ecological biomimetic culture of aerobic probiotics and rapid temperature-sensitive drying (≤50°C) culture materials that can protect probiotics to the greatest extent under relatively low temperature conditions. Bacteria activity, a solid ecological culture and drying machine that minimizes the rate of miscellaneous bacteria, the technical scheme is as follows:
一种固体生态培养、干燥一体机,其组成依次包括热风发生系统、液体输入装置、旋转物料处理系统、机架装置、旋风分离器9以及功能控制系统。An integrated machine for solid ecological cultivation and drying, which consists of a hot air generating system, a liquid input device, a rotating material processing system, a rack device, a cyclone separator 9 and a functional control system in sequence.
所述的热风发生系统包括鼓风装置1及与其连接的加热进风筒管装置2和进风管3。The hot air generating system includes a blower device 1 and a heating air inlet barrel device 2 and an air inlet pipe 3 connected thereto.
所述的鼓风装置1包括分别连接风机三通弯头1-3的培养中压风机1-2和干燥高压风机1-1,由风机架14支撑固定,风机三通弯头1-3出口与加热进风筒管装置2相连,培养中压风机1-2和干燥高压风机1-1,分别在进风口处设有空气滤清器,用于防止在培养、干燥过程中的杂菌感染。Described blower device 1 comprises respectively the cultivation medium pressure blower 1-2 and the dry high pressure blower 1-1 that connect blower fan tee elbow 1-3, is supported and fixed by blower frame 14, blower blower tee elbow 1-3 The outlet is connected to the heating air inlet pipe device 2, the medium pressure fan 1-2 for cultivation and the high pressure fan 1-1 for drying are respectively provided with air filters at the air inlets to prevent miscellaneous bacteria during the cultivation and drying process Infect.
所述的加热进风筒管装置2包括耐高温陶瓷内管2-2及其外部的不锈钢外管2-3,耐高温陶瓷内管2-2内设有电加热装置2-1,通过变径风管2-4以及进风管弯头2-5和进风管3与旋转物料处理系统相通,进风管弯头2-5用于连接变径风管2-4及进风管3且使得二者夹角呈90°,近鼓风装置1侧不锈钢外管2-3外表面设有接线盒2-6,分别与耐高温陶瓷内管2-2中的电加热装置2-1和功能控制系统电连接。The described heating air intake tube device 2 comprises a high temperature resistant ceramic inner tube 2-2 and an external stainless steel outer tube 2-3, and the high temperature resistant ceramic inner tube 2-2 is provided with an electric heating device 2-1, through which The diameter air pipe 2-4, the air inlet pipe elbow 2-5 and the air inlet pipe 3 communicate with the rotating material handling system, and the air inlet pipe elbow 2-5 is used to connect the variable diameter air pipe 2-4 and the air inlet pipe 3 And the angle between the two is 90°, and the outer surface of the stainless steel outer tube 2-3 near the blower device 1 is provided with a junction box 2-6, which is respectively connected to the electric heating device 2-1 in the high-temperature-resistant ceramic inner tube 2-2. It is electrically connected with the functional control system.
所述的液体输入装置包括液体输入泵15以及连接液体输入泵15与热风发生系统的进风管弯头2-5处的弯头液体输入器17,其中弯头液体输入器17的液管经进风管3管内伸入至自控旋转筒体6进风侧筒体端盖4内侧中心口,弯头液体输入器17的液体出口处设有热风流量分配器,热风流量分配器由凸型布满孔洞(孔洞直径一般为2mm)的圆板构成,其凸面朝向出风侧筒体端盖7方向,并由4根不锈钢管固定于进风侧筒体端盖4内侧中心口周围。通过热风流量分配器,既可使柱状风流被打散,有利于培养、干燥时的热风流呈现均匀、柔和状态;也可实现封闭式接种菌种液和物料自动加湿功能的均匀、柔和状态。工作时,液体输入泵15将伴随培养中压风机1-2以及自控旋转筒体6同步启动运转。The liquid input device includes a liquid input pump 15 and an elbow liquid input device 17 at the elbow 2-5 of the air inlet pipe connecting the liquid input pump 15 and the hot wind generating system, wherein the liquid pipe of the elbow liquid input device 17 passes through The air inlet pipe 3 extends into the inner center opening of the end cover 4 of the self-control rotating cylinder 6, and the liquid outlet of the elbow liquid input device 17 is provided with a hot air flow distributor. The hot air flow distributor is formed by a convex cloth. It is composed of a circular plate full of holes (the diameter of the hole is generally 2mm), and its convex surface faces the direction of the end cover 7 of the air outlet side, and is fixed around the center opening inside the end cover 4 of the air inlet side by 4 stainless steel tubes. Through the hot air flow distributor, the columnar air flow can be broken up, which is conducive to the uniform and soft state of the hot air flow during cultivation and drying; it can also realize the uniform and soft state of the closed inoculation seed liquid and the automatic humidification function of materials. During work, the liquid input pump 15 will start to run synchronously with the cultivation medium-pressure fan 1-2 and the self-control rotating cylinder 6 .
所述的电加热装置2-1位于加热进风筒管装置2的耐高温陶瓷内管2-2中,由65根六孔耐高温硅条2-1-4,2只梯形不锈钢钢条端架2-1-2,2只梯形钢条内架2-1-3和4根不锈钢长螺杆2-1-1以及相应若干螺母、垫片及填补空缺瓷管2-1-5构成半笼式凹形架,是由不锈钢长螺杆2-1-1分别穿过六孔耐高温硅条2-1-4两端孔,梯形钢条内架2-1-3,梯形不锈钢钢条端架2-1-2和填补空缺瓷管2-1-5及螺母进行固定,形成3面,每一面具有4孔,分别可供2根分别为5KW、220V耐高温电热丝2-1-6分别往返穿插孔中,共有6根耐高温电热丝2-1-6构成3面,分为2组,分别以相互间隔的3根耐高温电热丝2-1-6构成其中1组,每组各形成6个端头,在靠近鼓风装置1一侧的接线盒2-6中,采用星型连接法分别连接每组中耐高温电热丝的3路中3个端头,另3个端头分别与380V电源的3根火线连接,并接受功能控制系统的控制;当执行生态培养功能时,仅启动1组3根耐高温电热丝以及培养中压风机1-2和旋转筒体同步工作;当执行干燥功能时,将同时启动2组6根耐高温电热丝以及干燥高压风机1-1和自控旋转筒体同步工作。The electric heating device 2-1 is located in the high-temperature-resistant ceramic inner tube 2-2 of the heating air inlet tube device 2, and consists of 65 six-hole high-temperature-resistant silicon bars 2-1-4, and 2 trapezoidal stainless steel bar ends Frame 2-1-2, 2 trapezoidal steel bar inner frames 2-1-3 and 4 stainless steel long screws 2-1-1 and corresponding nuts, gaskets and porcelain tubes 2-1-5 to fill the vacancy constitute a half-cage The concave frame is made of stainless steel long screw rod 2-1-1 respectively passing through the holes at both ends of the six-hole high-temperature resistant silicon bar 2-1-4, the inner frame of the trapezoidal steel bar 2-1-3, and the end frame of the trapezoidal stainless steel bar 2-1-2 is fixed with the porcelain tube 2-1-5 and the nut to fill the vacancy, forming 3 sides, each side has 4 holes for 2 high temperature resistant heating wires 2-1-6 of 5KW and 220V respectively In the back-and-forth insertion hole, there are 6 high-temperature-resistant heating wires 2-1-6 to form 3 sides, which are divided into 2 groups, and 3 high-temperature-resistant heating wires 2-1-6 are separated from each other to form 1 group, and each group Each form 6 terminals, in the junction box 2-6 near the side of the blower device 1, use the star connection method to respectively connect 3 terminals in the 3 circuits of the high temperature resistance heating wire in each group, and the other 3 terminals The heads are respectively connected to the three live wires of the 380V power supply, and are controlled by the functional control system; when the ecological cultivation function is performed, only one group of three high-temperature-resistant electric heating wires is started, and the cultivation medium-pressure fan 1-2 and the rotating cylinder work synchronously ; When the drying function is performed, 2 groups of 6 high-temperature-resistant heating wires and the drying high-pressure fan 1-1 and the self-controlled rotating cylinder will be started to work synchronously.
所述的机架装置包括自控旋转筒体6下方的机架23和集料板装置22,发挥支撑及集料作用。The frame device includes a frame 23 below the self-controlled rotating cylinder 6 and a material collecting plate device 22 to play the role of support and material collection.
所述的旋转物料处理系统包括自控旋转筒体6,置于自控旋转筒体6中部的筒体进、出料装置5,自控旋转筒体6中空,两端由进风侧筒体端盖4和出风侧筒体端盖7加硅胶密封条密封,进风管3和出风管8分别于进风侧筒体端盖4和出风侧筒体端盖7中心处与自控旋转筒体6同心轴向连接;自控旋转筒体6外壁两端分别设有筒体旋转轨道13;动力主轴24和从动主轴21架于机架23两侧轴承座上,与自控旋转筒体6轴向平行,两根主轴上分别对称设有2个动力轴轮25和从动轴轮20,共4只轴轮,动力轴轮25,从动轴轮20与两道筒体旋转轨道13紧密接触,减速机16与机架23一侧动力主轴24以联轴器相联,提供旋转动力,通过2只动力轴轮25及与其紧密接触的两道筒体旋转轨道13带动自控旋转筒体6转动;而另一侧从动主轴21上的2只从动轴轮20则作为自控旋转筒体6的从动支撑随自控旋转筒体6转动;进风侧筒体端盖4和出风侧筒体端盖7中心与动力轴轮25和从动轴轮20中心的连线呈90°夹角;可保持转筒稳定性。减速机16与功能控制系统电连接,由功能控制系统控制其开启或停息;自控旋转筒体6的周期性旋转和停息通过减速机由功能控制系统的时控仪任意设定调节。The rotary material handling system includes a self-controlled rotating cylinder 6, a cylinder inlet and outlet device 5 placed in the middle of the self-controlled rotating cylinder 6, the self-controlled rotating cylinder 6 is hollow, and the two ends are covered by the cylinder end caps 4 on the air inlet side. and air outlet side cylinder end cover 7 plus silicone sealing strip seal, the air inlet pipe 3 and the air outlet pipe 8 are respectively connected to the center of the air inlet side cylinder end cover 4 and the air outlet side cylinder end cover 7 and the self-controlled rotating cylinder 6 concentric and axial connections; the two ends of the outer wall of the self-controlled rotating cylinder 6 are respectively provided with a cylinder rotating track 13; Parallel, two power shaft wheels 25 and driven shaft wheels 20 are respectively symmetrically arranged on the two main shafts, a total of 4 shaft wheels, the power shaft wheel 25, and the driven shaft wheel 20 are in close contact with the two cylinder rotating tracks 13, The reducer 16 is connected with the power main shaft 24 on one side of the frame 23 by a coupling to provide rotational power, and the self-controlled rotating cylinder 6 is driven to rotate through two power shaft wheels 25 and two cylinder rotating tracks 13 in close contact with them; On the other side, the two driven shaft wheels 20 on the driven main shaft 21 are used as the driven support of the self-controlled rotating cylinder 6 to rotate with the self-controlled rotating cylinder 6; The connecting line between the center of the end cover 7 and the center of the power shaft wheel 25 and the center of the driven shaft wheel 20 is at an angle of 90°; the stability of the drum can be maintained. The speed reducer 16 is electrically connected with the function control system, and is controlled by the function control system to open or stop; the periodic rotation and stop of the self-controlled rotating cylinder 6 is arbitrarily set and adjusted by the time controller of the function control system through the speed reducer.
所述的自控旋转筒体6两侧的进风侧筒体端盖4和出风侧筒体端盖7均设有端盖双轴连接器19和端盖锁紧器18,用于端盖的开闭和固定;进风侧筒体端盖4和出风侧筒体端盖7内侧面均设有梯形密封槽,外窄里宽,镶嵌有10mm方型硅胶密封条(方形硅条的尺寸一般为10mm),可与自控旋转筒体6两侧法兰端面紧贴,保证进风侧筒体端盖4和出风侧筒体端盖7关闭时的密封性能;出风侧筒体端盖7法兰的两侧分别设有固定圆环,其下方机架23两侧分别设有筒体法兰连杆拉紧钩26,在进料或打开端门之前,必须以筒体法兰连杆拉紧钩26拉紧固定环,用于固定自控旋转筒体6,防止其转动以及端盖7异位翻开。Both the air inlet side cylinder end cap 4 and the air outlet side cylinder end cap 7 on both sides of the self-controlled rotating cylinder 6 are provided with an end cap biaxial connector 19 and an end cap locker 18 for the end cap The opening, closing and fixing of the air inlet side cylinder end cover 4 and the air outlet side cylinder end cover 7 are equipped with trapezoidal sealing grooves, which are narrow on the outside and wide on the inside, inlaid with 10mm square silicone sealing strips (square silicon strips) The size is generally 10mm), which can be closely attached to the end faces of the flanges on both sides of the self-controlled rotating cylinder 6 to ensure the sealing performance when the cylinder end cover 4 on the air inlet side and the cylinder end cover 7 on the air outlet side are closed; the cylinder on the air outlet side The two sides of the flange of the end cover 7 are respectively provided with fixed rings, and the two sides of the lower frame 23 are respectively provided with cylinder flange connecting rod tension hooks 26. Before feeding or opening the end door, the cylinder method must be used to The blue connecting rod tightening hook 26 tightens the fixing ring, which is used to fix the self-controlled rotating cylindrical body 6 to prevent it from rotating and the end cover 7 from being opened in an abnormal position.
所述减速机16的电动机风叶罩上还可设有一旋转圆板,用于在停止减速机工作状态下,可轻松转动电动机风叶,通过转动主轴可调整控制自控旋转筒体6进、出料装置6及两侧端盖4,7于指定位置姿态,便于进料及开、闭筒体端盖。The fan blade cover of the motor of the reducer 16 can also be provided with a rotating circular plate, which is used to easily rotate the fan blade of the motor when the reducer is stopped, and can adjust and control the automatic rotation cylinder 6 to enter and exit The feeding device 6 and the end caps 4 and 7 on both sides are in a designated position and posture, which is convenient for feeding and opening and closing the end caps of the cylinder body.
所述的自控旋转筒体6筒内壁两对侧轴向处分别各设有一搅料板6-1,两搅料板沿轴向中心线各彼此相反倾斜5°并分别与自控旋转筒体6筒内壁垂直,筒体旋转时可改善物料的轴向往返运动的搅拌效果;与两对侧分布搅料板各相差90°角的自控旋转筒体6筒内壁处的两对侧轴向位置,分别各设有一碎料器6-2,每一碎料器分别由6块径向站立正梯形碎料板6-3组成,各自距离均匀并轴向直线分布,其每一正梯形碎料板均与筒内壁径向、轴向呈两个方向偏斜6°,每一碎料器6-2中点两侧各3块正梯形碎料板6-3的径向及轴向的偏斜方向相反;这种复合倾斜方式和形状可有效碎料以最大限度避免物料球形结块和提高物料搅拌效果。The inner walls of the self-controlled rotating cylinder 6 are respectively provided with a stirring plate 6-1 at the axial positions on both sides of the inner wall. The inner wall of the cylinder is vertical, and the mixing effect of the axial reciprocating movement of the material can be improved when the cylinder rotates; the two pairs of side axial positions at the inner wall of the self-controlled rotating cylinder 6, which are 90° apart from the two pairs of side distribution stirring plates, Each is provided with a crushing device 6-2, and each crushing device is composed of 6 radially standing positive trapezoidal particle boards 6-3. Both are deflected by 6° in two directions radially and axially with the inner wall of the cylinder, and the radial and axial deflection of three regular trapezoidal particle boards 6-3 on both sides of the midpoint of each crusher 6-2 The direction is opposite; this composite tilting mode and shape can effectively crush the material to avoid the spherical agglomeration of the material to the greatest extent and improve the mixing effect of the material.
所述的出风侧筒体端盖7内侧中心孔处设有迷宫格状的粉料阻挡器,并通过4根不锈钢管固定于出风侧筒体端盖7内侧出风管8管口四周边;可造成热风流回旋曲折路径的流动方式阻挡热风流中粉料逃逸。A labyrinth-like powder blocker is provided at the center hole inside the cylinder end cover 7 on the air outlet side, and is fixed to the air outlet pipe 8 inside the cylinder end cover 7 on the air outlet side by four stainless steel tubes. Periphery; the flow mode that can cause the hot air flow to turn and tortuous path prevents the powder in the hot air flow from escaping.
所述的进、出料装置5设有进、出料门,进、出料门一般设计成长方形,长方形进、出料门的水平一侧采用双门轴连接于自控旋转筒体6,可水平内推打开或反向关闭。进、出料门外侧面设有硅胶板具有关闭时的密封功能,此处设有螺栓锁紧装置用于进、出料门关闭时固定进、出料门于自控旋转筒体6筒壁门框;自控旋转筒体6筒壁门框上另设有一短螺杆用于进、出料门打开时与自控旋转筒体6筒壁保持呈径向90°状态固定,用于自动出料,另外配有活动进料导板置于进、出料门处,用于进料。The inlet and outlet device 5 is provided with inlet and outlet doors, the inlet and outlet doors are generally designed to be rectangular, and the horizontal side of the rectangular inlet and outlet doors is connected to the self-control rotating cylinder 6 by double door shafts, which can Push in horizontally to open or reverse to close. There is a silicone plate on the outer side of the inlet and outlet doors, which has a sealing function when it is closed. There is a bolt locking device here to fix the inlet and outlet doors on the 6-wall door frame of the self-controlled rotating cylinder when the inlet and outlet doors are closed. ; There is also a short screw rod on the door frame of the self-controlled rotating cylinder 6, which is used to maintain a radial 90° state with the wall of the self-controlled rotating cylinder 6 when the inlet and outlet doors are opened, and is used for automatic discharging. The movable feeding guide plate is placed at the inlet and outlet doors for feeding.
所述的出风管8的直径大于进风管3的直径;进风管3及出风管8各一端分别与自控旋转筒体6两侧的进风侧筒体端盖4和出风侧筒体端盖7中心轴承同心、轴向连接,进风管3及出风管8的另两端分别与进风管弯头2-5和旋风分离筒体11进风口之间采用法兰活动套管方式安全性连接。采用这样的连接方式可以保证自控旋转筒体6旋转过程的安全性,确保其他部件不会由于自控旋转筒体6的旋转过程中可能发生的转动不灵活而移位。The diameter of the air outlet pipe 8 is greater than the diameter of the air inlet pipe 3; each end of the air inlet pipe 3 and the air outlet pipe 8 is respectively connected to the air inlet side cylinder end cover 4 and the air outlet side of the self-controlled rotating cylinder 6 sides. The center bearing of cylinder end cover 7 is connected concentrically and axially, and the other two ends of air inlet pipe 3 and air outlet pipe 8 are respectively connected with elbows 2-5 of air inlet pipe and the air inlet of cyclone separation cylinder 11 with flange activities Sleeve way safety connection. Adopting such a connection method can ensure the safety of the rotation process of the self-controlled rotating cylinder 6 and ensure that other components will not be displaced due to inflexible rotation that may occur during the rotation of the self-controlled rotating cylinder 6 .
所述的旋风分离器包括旋风分离筒体11及其上部的分离筒中心管道10,下部的锥形筒体12,自控旋转筒体6的出风管8与旋风分离筒体11在圆周相切处联接相通,连接管道处设有电热耦、湿度感受器和温度表。出风风流沿旋风分离筒体内壁高速旋转,可使少量逃逸粉料随旋转风流沿锥形筒体旋转下降而收集,出风风流从旋风分离筒体11上部的分离筒中心管道10排出。The cyclone separator includes a cyclone separation cylinder 11 and a separation cylinder central pipe 10 on its upper part, a conical cylinder 12 on the lower part, and the air outlet pipe 8 of the self-controlled rotating cylinder 6 is tangent to the cyclone separation cylinder 11 on the circumference. The connecting pipes are connected with each other, and the connecting pipes are equipped with electric thermocouples, humidity sensors and thermometers. The outlet air flow rotates at high speed along the inner wall of the cyclone separation cylinder, so that a small amount of escaped powder can be collected by rotating and descending along the conical cylinder with the rotation air flow.
所述的功能控制系统与鼓风装置1、电加热装置2-1、液体输入泵15、电热耦、湿度感受器、减速机16电联接,并通过微电脑控制器智能化控制上述各部件的工作与停止。当电加热装置2-1停止工作,而鼓风装置1仍然继续工作一段时间,其延迟工作的时间可由时控仪任意设定调节。The functional control system is electrically connected with the blower device 1, the electric heating device 2-1, the liquid input pump 15, the electric thermocouple, the humidity sensor, and the speed reducer 16, and intelligently controls the work and operation of the above-mentioned components through a microcomputer controller. stop. When the electric heating device 2-1 stops working, and the blower device 1 still continues to work for a period of time, the time for delaying work can be adjusted arbitrarily by the time controller.
利用本发明所提供的一种固体生态培养、干燥一体机可实现培养与干燥功能的切换,当执行生态培养功能时,仅启动1组3根耐高温电热丝2-1-6以及培养中压风机1-2和自控旋转筒体6同步工作;当执行干燥功能时,将同时启动2组6根耐高温电热丝2-1-6以及干燥高压风机1-1和自控旋转筒体6同步工作。A solid ecological cultivation and drying integrated machine provided by the present invention can be used to switch between cultivation and drying functions. When performing the ecological cultivation function, only one group of three high-temperature-resistant heating wires 2-1-6 and the cultivation medium voltage are activated. The fan 1-2 and the self-controlled rotating cylinder 6 work synchronously; when performing the drying function, 2 groups of 6 high-temperature-resistant electric heating wires 2-1-6 and the drying high-pressure fan 1-1 and the self-controlled rotating cylinder 6 will work synchronously .
本发明主要功能特点如下:Main functional characteristics of the present invention are as follows:
在同一设备中一次性完成益生菌生态有氧培养以及高效温敏物料干燥,能够突现功效的最大化,有效抗杂菌感染,突显一体机优势。The ecological aerobic cultivation of probiotics and the drying of high-efficiency temperature-sensitive materials can be completed at one time in the same equipment, which can maximize the efficacy, effectively resist miscellaneous bacterial infections, and highlight the advantages of the all-in-one machine.
1、有氧生物发酵的生态化培养1. Ecological cultivation of aerobic fermentation
各相关功能的协调配合可满足益生菌生态化培育要求,由时控仪调控的旋转筒体工作,可以最适的物料翻搅强度使物料充分获氧,充分实现有氧生物发酵程度均匀,温控系统和湿度控制系统通过风力分配器,可使转筒内物料处于温、湿度适宜的仿生态培养环境中。The coordination and cooperation of various related functions can meet the requirements of ecological cultivation of probiotics. The rotating cylinder regulated by the time controller can work with the most suitable stirring intensity to make the materials fully obtain oxygen, fully realize the uniform degree of aerobic biological fermentation, and the temperature The control system and the humidity control system can make the materials in the drum be in an ecologically imitative cultivation environment with suitable temperature and humidity through the wind distributor.
2、液体菌种封闭式接种方式安全、快速、均匀、简便2. The closed inoculation method of liquid bacteria is safe, fast, uniform and simple
可在封闭无菌状态下,将菌种液通过接种系统、风力分配器和自动翻搅系统的协同工作,可将菌种液均匀接种混合于培养物料。其特点表现为:安全、快速、均匀、简便。In a closed sterile state, the seed liquid can be inoculated and mixed with the culture material evenly through the cooperative work of the inoculation system, the wind distributor and the automatic stirring system. Its characteristics are as follows: safe, fast, even and simple.
3、合理的温敏干燥效率高,有助于保持益生菌活性和数量3. Reasonable temperature-sensitive drying with high efficiency helps to maintain the activity and quantity of probiotics
在热风发生系统,旋转物料处理系统,旋风分离器同时工作状态下,43±2℃的快速低温风流干燥可高效率带走培养物料中的水分,达到快速温敏干燥但能维持菌体活性的理想效果。When the hot air generation system, the rotating material handling system and the cyclone separator are working at the same time, the rapid low-temperature air drying at 43±2°C can efficiently remove the moisture in the culture material, achieving rapid temperature-sensitive drying but maintaining the activity of the bacteria Ideal effect.
本申请专利产品根据不同生产目的,可改变相关的工作参数,工厂规模化生产制备不同的好氧益生菌以及特定菌质,是绿色功能性动物微生物饲料添加剂生产的关键设备,也是医药生物制药、食品发酵生产,以及涉及到有氧菌群固体发酵培养生产的相关领域提高产品质量和效率的新型设备。The patented product of this application can change the relevant working parameters according to different production purposes. The factory produces large-scale production of different aerobic probiotics and specific bacteria. Food fermentation production, as well as new equipment for improving product quality and efficiency in related fields involving aerobic flora solid fermentation culture production.
本发明适用于好氧益生菌类固体培养与温敏干燥一体化的工厂规模化生产。The invention is suitable for large-scale production in factories integrating aerobic probiotic solid culture and temperature-sensitive drying.
本发明发酵容量可为2500L。一体机采用304号不锈钢材料以及微电脑自动控制技术,从仿生学角度研制相关结构,能够自动化控制有氧生物发酵和温敏干燥过程的相关环节,提供良好通风和适宜温、湿度条件,确保益生菌群的高效培育,并能有效地达到较好的封闭性能,使杂菌率控制在1%以下。通过湿度自控及菌液接种输入系统可安全封闭式定量接种菌种液(接种量占培养底物的20%)。本申请专利产品能够实行一机多功能化,简便高效,当完成益生菌有氧生物发酵培养工作后,在原有培养旋转筒体中,可连续性完成温敏、快速、热风干燥工作,使发酵物料含水量减少至10%以下,并能有效保持培育菌群的生物学活性和高质量菌株芽孢数量。The fermentation capacity of the present invention can be 2500L. The all-in-one machine adopts No. 304 stainless steel material and microcomputer automatic control technology, and develops related structures from the perspective of bionics, which can automatically control the relevant links of aerobic biological fermentation and temperature-sensitive drying process, provide good ventilation and suitable temperature and humidity conditions, and ensure the probiotics The high-efficiency cultivation of the group can effectively achieve better sealing performance, so that the rate of miscellaneous bacteria can be controlled below 1%. Through the automatic humidity control and bacterial liquid inoculation input system, the bacterial seed liquid can be inoculated quantitatively in a closed and safe manner (the inoculum amount accounts for 20% of the culture substrate). The patented product of this application can be multi-functional in one machine, which is simple and efficient. After the aerobic fermentation of probiotics is completed, the temperature-sensitive, fast, and hot-air drying can be continuously completed in the original rotating cylinder to make the fermentation The water content of the material is reduced to less than 10%, and the biological activity of the cultivated flora and the number of high-quality strain spores can be effectively maintained.
本产品采用转筒式内置挡料板搅拌物料,旋转筒体内侧壁分别设有两侧反向倾斜性搅料板和其相差90℃轴向分布的碎料板,以加强固体培养-干燥物料的径向、轴向翻搅和混合功能。转筒端盖采用双轴结构保证灵活开闭和较好的密封性能。进、出风管各自两端分别连接端盖中心轴承和法兰活动套管,该连接方式可保证旋转筒体转动时不影响其他部件,性能安全可靠。热风发生系统的加热采用独特快速风加热结构,配以风机组合和温敏控制系统,满足好氧益生菌类生态培养条件和培养固体物料干燥的快速温敏需求,采用微电脑温、湿度控制仪自动调节控制转筒内适宜的温、湿度及搅拌等相关功能。出风管接一旋风分离器。回收逃逸粉尘,出风口无可见液滴。This product uses a drum-type built-in baffle to stir the material. The inner wall of the rotating cylinder is equipped with reversely inclined stirring plates on both sides and a particle board with a difference of 90°C in the axial direction to strengthen the solid cultivation-drying of the material. Radial and axial stirring and mixing functions. The end cover of the drum adopts a biaxial structure to ensure flexible opening and closing and better sealing performance. The two ends of the inlet and outlet pipes are respectively connected to the central bearing of the end cover and the movable sleeve of the flange. This connection method can ensure that the rotating cylinder does not affect other components, and the performance is safe and reliable. The heating of the hot air generating system adopts a unique rapid air heating structure, equipped with a fan combination and a temperature-sensitive control system, which meets the ecological cultivation conditions of aerobic probiotics and the rapid temperature-sensitive demand for drying solid materials. It adopts a microcomputer temperature and humidity controller to automatically Adjust and control the appropriate temperature, humidity and stirring and other related functions in the drum. The air outlet pipe is connected with a cyclone separator. The escaped dust is recovered, and there is no visible droplet at the air outlet.
附图说明Description of drawings
图1是固体生态培养、干燥一体机结构示意图的主视图;Fig. 1 is the front view of the structural schematic diagram of the solid ecological cultivation and drying all-in-one machine;
图2是固体生态培养、干燥一体机旋转物料处理系统及机架装置结构示意图的左视图;Fig. 2 is a left view of the solid ecological cultivation and drying integrated machine rotary material handling system and the structural diagram of the rack device;
图3是固体生态培养、干燥一体机热风发生系统结构示意图的主视图;Fig. 3 is the front view of the structural diagram of the hot air generation system of the solid ecological cultivation and drying integrated machine;
图4是固体生态培养、干燥一体机电加热装置结构示意图的主视图;Fig. 4 is a front view of a schematic structural diagram of a solid ecological cultivation and drying integrated electromechanical heating device;
图5是固体生态培养、干燥一体机电加热装置结构示意图的右视图;Fig. 5 is the right view of the structural schematic diagram of the solid ecological cultivation and drying integrated electromechanical heating device;
图6是固体生态培养、干燥一体机自控旋转筒体内筒壁结构示意图的主视图;Fig. 6 is a front view of the structure diagram of the inner cylinder wall of the self-controlled rotating cylinder of the solid ecological cultivation and drying integrated machine;
图7是固体生态培养、干燥一体机自控旋转筒体内筒壁结构示意图的展开图;Fig. 7 is an expanded view of the structure diagram of the inner cylinder wall of the self-controlled rotating cylinder of the solid ecological cultivation and drying integrated machine;
图8是固体生态培养、干燥一体机自控旋转筒体内筒壁展开图中碎料器结构示意图的右视图;Fig. 8 is a right view of the structural diagram of the breaker in the expanded view of the inner cylinder wall of the self-controlled rotating cylinder of the solid ecological cultivation and drying integrated machine;
图9是固体生态培养、干燥一体机功能控制系统电路图。SS1是干燥温控,SS2是干燥搅拌时控,SS3是干燥通风,SS4是干燥搅拌,SS5培养温控,SS6培养湿控,SS7是搅拌时控,SS8是风机时控。Fig. 9 is a circuit diagram of the functional control system of the solid ecological cultivation and drying all-in-one machine. SS1 is drying temperature control, SS2 is drying stirring time control, SS3 is drying ventilation, SS4 is drying stirring, SS5 cultivation temperature control, SS6 cultivation humidity control, SS7 is stirring time control, SS8 is fan time control.
其中,1-鼓风装置;1-1-干燥高压风机;1-2-培养中压风机;1-3-风机三通弯头;2-加热进风筒管装置;2-1-电加热装置;2-1-1-长螺杆(4根);2-1-2-端架(2只);2-1-3-内架(2只);2-1-4-耐高温硅条(65只);2-1-5-瓷管;2-1-6-耐高温电热丝(6根);2-2-耐高温陶瓷内管;2-3-不锈钢外管;2-4-变径风管;2-5-进风管弯头;2-6-接线盒;3-进风管;4-进风侧筒体端盖;5-筒体进、出料装置;6-自控旋转筒体;6-1-搅料板(2块);6-2-碎料器(2套);6-3-碎料板(6块/套);7-出风侧筒体端盖;8-出风管;9-旋风分离器;10-分离筒中心管道;11-旋风分离筒体;12-锥形筒体;13-筒体旋转轨道(2);14-风机架;15-液体输入泵;16-减速机;17-弯头液体输入控制器;18-端盖锁紧器(6套);19-端盖双轴连接器;20-从动轴轮(2只);21-从动主轴;22-集料板装置;23-机架;24-动力主轴;25-动力轴轮(2只);26-筒体法兰连杆拉紧钩。Among them, 1-blowing device; 1-1-drying high-pressure fan; 1-2-cultivation medium-pressure fan; 1-3-fan three-way elbow; 2-heating air inlet tube device; 2-1-electric heating Device; 2-1-1-long screw (4 pieces); 2-1-2-end frame (2 pieces); 2-1-3-inner frame (2 pieces); 2-1-4-high temperature resistant silicon Strips (65 pieces); 2-1-5-porcelain tube; 2-1-6-high temperature resistant electric heating wire (6 pieces); 2-2-high temperature resistant ceramic inner tube; 2-3-stainless steel outer tube; 2- 4-Reduced air duct; 2-5-Elbow of air inlet pipe; 2-6-Junction box; 3-Inlet pipe; 4-Cylinder end cover on the air inlet side; 5-Cylinder inlet and outlet device; 6-Automatically controlled rotating cylinder; 6-1-Stirring board (2 pieces); 6-2-Crusher (2 sets); 6-3-Particle board (6 pieces/set); 7-Outlet side Cylinder end cover; 8-air outlet pipe; 9-cyclone separator; 10-central pipe of separation cylinder; 11-cyclone separation cylinder; 12-conical cylinder; 13-cylinder rotating track (2); 14- Fan frame; 15-liquid input pump; 16-reducer; 17-elbow liquid input controller; 18-end cover locker (6 sets); 19-end cover biaxial connector; 20-driven shaft Wheel (2); 21-driven main shaft; 22-collecting plate device; 23-frame; 24-power main shaft; 25-power shaft wheel (2); 26-tube flange connecting rod tension hook .
图10-图15是具体实施方式的附图。10-15 are drawings of specific embodiments.
图10是解淀粉芽孢杆菌3批固体生态培养平均pH动态变化图;Fig. 10 is 3 batches of solid ecological cultivation average pH dynamic change figure of bacillus amyloliquefaciens;
图11是解淀粉芽孢杆菌3批固体生态培养平均温度动态变化图;Fig. 11 is 3 batches of solid ecological culture average temperature dynamic change figure of bacillus amyloliquefaciens;
图12是解淀粉芽孢杆菌3批固体生态培养平均湿度动态变化图;Fig. 12 is 3 batches of Bacillus amyloliquefaciens solid ecological culture average humidity dynamic change figure;
图13是解淀粉芽孢杆菌3批固体生态培养平均活菌数动态变化图;Fig. 13 is 3 batches of Bacillus amyloliquefaciens 3 batches of solid ecological culture average live bacterial number dynamic change figure;
图14是解淀粉芽孢杆菌3批固体生态培养物干燥后含水量;Figure 14 is the moisture content after 3 batches of solid ecological cultures of Bacillus amyloliquefaciens are dried;
图15是解淀粉芽孢杆菌3批固体生态培养物干燥后芽孢率。Fig. 15 is the spore rate after drying of 3 batches of solid ecological cultures of Bacillus amyloliquefaciens.
具体实施方式Detailed ways
实施例1Example 1
以容量2500L,其设备自重约3吨为例。本发明固体生态培养、干燥一体机结构结构如图1-2所示,由热风发生系统,液体输入装置,旋转物料处理系统,机架装置,旋风分离器,功能控制系统所组成。Take the capacity of 2500L, the weight of the equipment is about 3 tons as an example. The structure of the solid ecological cultivation and drying integrated machine of the present invention is shown in Figure 1-2, which is composed of a hot air generating system, a liquid input device, a rotating material processing system, a rack device, a cyclone separator, and a function control system.
1、热风发生系统1. Hot air generating system
热风发生系统如图3包括鼓风装置1及与其连接的加热进风筒管装置2和进风管3。The hot air generating system as shown in FIG. 3 includes a blower device 1 and a heating air inlet barrel device 2 and an air inlet pipe 3 connected thereto.
鼓风装置1包括培养中压风机1-2和干燥高压风机1-1分别连接风机三通弯,1-3,由风机架14支撑固定,风机三通弯1-3出口再与加热进风筒管装置2相连。两风机进风口处设有空气滤清器,用于防止在培养、干燥过程中的杂菌感染。Blowing device 1 includes medium-pressure fan 1-2 for cultivation and high-pressure drying fan 1-1 respectively connected to fan tee bend, 1-3, supported and fixed by fan frame 14, and the outlet of fan tee bend 1-3 is connected with heating The blower tube device 2 is connected. Air filters are installed at the air inlets of the two fans to prevent bacterial infection during the cultivation and drying process.
加热进风筒管装置2包括由耐高温陶瓷内管2-2和不锈钢外管2-3构成,近风机侧不锈钢外管2-3外表面设有接线盒2-6,耐高温陶瓷内管2-2内设有电加热装置2-1。通过变径风管2-4以及进风管弯头2-5和进风管3与旋转物料处理系统相通。The heating air inlet tube device 2 includes a high temperature resistant ceramic inner tube 2-2 and a stainless steel outer tube 2-3, the outer surface of the stainless steel outer tube 2-3 near the fan side is provided with a junction box 2-6, and the high temperature resistant ceramic inner tube 2-2 is provided with an electric heating device 2-1. It communicates with the rotating material handling system through the variable diameter air pipe 2-4, the air inlet pipe elbow 2-5 and the air inlet pipe 3.
如图4、图5,电加热装置2-1位于加热进风筒管装置2的耐高温陶瓷内管2-2中,由六孔耐高温硅条和梯形钢条构成的半笼式凹形架形成3面,用4根不锈钢螺杆及螺丝分别穿过硅条两端孔固定,每一面具有4孔,可供2根分别为5KW,220V耐高温电热丝分别往返穿插其中,共有6根耐高温电热丝构成3面,分为2组,分别以相互间隔的3根耐高温电热丝构成其中1组,每组各形成6个端头,在靠近风机一侧,分别采用星型连接法连接并与380V电源接通,接受功能控制系统的控制。当执行生态培养功能时,仅启动1组3根耐高温电热丝以及培养中压风机1-2和旋转筒体同步工作;当执行干燥功能时,将同时启动2组6根耐高温电热丝以及干燥高压风机1-1和自控旋转筒体同步工作。As shown in Figure 4 and Figure 5, the electric heating device 2-1 is located in the high-temperature resistant ceramic inner tube 2-2 of the heating air inlet tube device 2, and is a semi-cage concave shape composed of six-hole high-temperature resistant silicon strips and trapezoidal steel strips. The frame forms 3 sides, and is fixed with 4 stainless steel screws and screws through the holes at both ends of the silicon strip. Each side has 4 holes, which can be used for 2 5KW, 220V high-temperature resistant heating wires to go back and forth, and there are 6 resistant The high-temperature heating wires constitute 3 sides, which are divided into 2 groups. One group is composed of 3 high-temperature-resistant heating wires spaced apart from each other. Each group forms 6 terminals. On the side close to the fan, they are connected by star connection method. And connect with 380V power supply, accept the control of function control system. When performing the function of ecological cultivation, only one group of 3 high-temperature-resistant electric heating wires and the cultivation medium-pressure fan 1-2 will work synchronously with the rotating cylinder; when performing the drying function, two groups of six high-temperature-resistant electric heating wires and Drying high-pressure blower 1-1 works synchronously with the self-controlled rotating cylinder.
2、液体输入装置2. Liquid input device
液体输入装置包括液体输入泵15的输出管连接至热风发生系统的进风管弯头2-5处的弯头液体输入器17,其中有液管经进风管3管内伸入至自控旋转筒体6进风侧筒体端盖4内侧中心口,通过热风流量分配器,既可使柱状风流被打散,有利于培养、干燥时的热风流呈现均匀、柔和状态;也可实现封闭式接种菌种液和物料自动加湿功能的均匀、柔和状态。工作时,液体输入泵15将伴随培养中压风机1-2以及自控旋转筒体6同步启动运转。热风流量分配器由凸型布满直径2mm孔洞的圆板构成,凸面朝向出风侧筒体端盖7方向,并由4根不锈钢管固定于进风侧筒体端盖4内侧中心口周围。The liquid input device includes the output pipe of the liquid input pump 15 connected to the elbow liquid input device 17 at the elbow 2-5 of the air inlet pipe of the hot air generating system, wherein a liquid pipe extends into the self-control rotating cylinder through the air inlet pipe 3 Body 6 The central opening of the inner side of the cylinder end cover 4 on the air inlet side, through the hot air flow distributor, can not only break up the columnar air flow, but also facilitate the uniform and soft state of the hot air flow during cultivation and drying; it can also realize closed inoculation Uniform and soft state of the seed liquid and material automatic humidification function. During work, the liquid input pump 15 will start to run synchronously with the cultivation medium-pressure fan 1-2 and the self-control rotating cylinder 6 . The hot air flow distributor is composed of a convex circular plate covered with holes with a diameter of 2mm. The convex surface faces the direction of the end cover 7 of the cylinder on the air outlet side, and is fixed around the center opening inside the end cover 4 of the cylinder on the air inlet side by 4 stainless steel tubes.
3、旋转物料处理系统3. Rotating material handling system
旋转物料处理系统包括自控旋转筒体6,如图6-8,置于自控旋转筒体6中部的筒体进、出料装置5,自控旋转筒体6中空,两端由进风侧筒体端盖4和出风侧筒体端盖7加硅胶密封条密封,进风管3和出风管8分别与两侧端盖4,7中心处与自控旋转筒体6同心轴向连接;自控旋转筒体6外壁两端分别设有筒体旋转轨道13,动力主轴24和从动主轴21架于机架两侧轴承座上,与自控旋转筒体6轴向平行,两根主轴上分别对称设有2个动力轴轮25和从动轴轮20,共4只轴轮25,20与两道筒体旋转轨道13紧密接触。减速机16与机架一侧动力主轴24以联轴器相联,提供旋转动力,通过2只动力轴轮25与两道筒体旋转轨道13紧密接触带动自控旋转筒体6转动,而另一侧从动主轴21上的2只从动轴轮20则作为从动支撑和转动。筒中心与两侧主轴轮呈90°夹角,可保持转筒稳定性。自控旋转筒体6的周期性旋转和停息由功能控制系统的时控仪任意设定调节。The rotary material handling system includes a self-controlled rotating cylinder 6, as shown in Figure 6-8, the cylinder inlet and outlet device 5 placed in the middle of the self-controlled rotating cylinder 6, the self-controlled rotating cylinder 6 is hollow, and the two ends are connected by the air inlet side cylinder The end cover 4 and the end cover 7 of the cylinder on the air outlet side are sealed with a silicone sealing strip, and the air inlet pipe 3 and the air outlet pipe 8 are respectively connected to the centers of the end covers 4 and 7 on both sides and the self-controlled rotating cylinder 6 in the axial direction; The two ends of the outer wall of the rotating cylinder 6 are respectively provided with a cylinder rotating track 13, the power spindle 24 and the driven spindle 21 are mounted on the bearing seats on both sides of the frame, parallel to the axis of the self-controlled rotating cylinder 6, and the two spindles are respectively symmetrical There are 2 power shaft wheels 25 and driven shaft wheels 20, a total of 4 shaft wheels 25, 20 are in close contact with the two cylinder rotating tracks 13. The reducer 16 is connected with the power main shaft 24 on one side of the frame by a coupling to provide rotational power, and the self-controlled rotating cylinder 6 is driven to rotate through the close contact between the two power shaft wheels 25 and the two cylinder rotating tracks 13, while the other 2 driven shaft wheels 20 on the side driven main shaft 21 are then used as driven support and rotation. The center of the drum forms a 90° angle with the main shaft wheels on both sides, which can maintain the stability of the drum. The periodic rotation and pause of the self-controlled rotating cylinder 6 are arbitrarily set and regulated by the time controller of the functional control system.
所述的自控旋转筒体6两侧的进风侧筒体端盖4和出风侧筒体端盖7均设有端盖双轴连接器19和端盖锁紧器19,用于端盖开闭和固定。两侧端盖4,7内侧面均设有梯形密封槽,外窄里宽,镶嵌有10mm方型硅胶密封条,可与自控旋转筒体6两侧法兰端面紧贴,保证两侧端盖4,7关闭时的闭封性能;出风侧筒体端盖7一侧的法兰设有两只固定圆环,其下方机架两侧分别设有筒体法兰连杆拉紧钩26,在进料或打开端门之前,必须以筒体法兰连杆拉紧钩26拉紧固定环,用于固定自控旋转筒体6,防止其转动以及端盖7异位翻开。Both the air inlet side cylinder end cap 4 and the air outlet side cylinder end cap 7 on both sides of the self-controlled rotating cylinder 6 are provided with an end cap biaxial connector 19 and an end cap locker 19 for the end cap Open and close and fixed. Both end covers 4 and 7 on both sides are equipped with trapezoidal sealing grooves, which are narrow outside and wide inside, inlaid with 10mm square silicone sealing strips, which can be closely attached to the end faces of the flanges on both sides of the self-controlled rotating cylinder 6 to ensure that the end covers on both sides 4,7 Sealing performance when closed; two fixed rings are provided on the flange on one side of the cylinder end cover 7 on the air outlet side, and cylinder flange connecting rod tensioning hooks 26 are respectively provided on both sides of the lower frame , Before feeding or opening the end door, the fixing ring must be tightened with the cylinder flange connecting rod tightening hook 26 to fix the self-controlled rotating cylinder 6 to prevent its rotation and the end cover 7 from being opened in an abnormal position.
减速机16的电动机风叶罩上还设有一旋转圆板,用于在停止减速机工作状态下,可轻松转动电动机风叶,通过转动主轴可调整控制自控旋转筒体6进、出料装置5及两侧端盖4,7于指定位置姿态,便于进料及开、闭筒体端盖。The motor fan blade cover of the reducer 16 is also provided with a rotating circular plate, which is used to easily rotate the motor fan blade when the reducer is stopped, and can adjust and control the self-controlled rotating cylinder 6 into and out of the material device 5 by rotating the main shaft. And the end caps 4 and 7 on both sides are in the designated position and posture, which is convenient for feeding materials and opening and closing the end caps of the cylinder body.
自控旋转筒体6筒内壁两对侧轴向处各设有一搅料板,两搅料板各相互相反倾斜6°,筒体旋转时可改善物料的径向、轴向搅拌效果;其相差90°角的自控旋转筒体6筒内壁处两对侧轴向位置,分别于径向各立有4块正梯形碎料板均匀距离、直线分布,可避免物料结块。Self-controlled rotating cylinder 6. There is a stirring plate on the two opposite sides of the inner wall of the cylinder. The two stirring plates are inclined 6° opposite to each other. When the cylinder rotates, the radial and axial stirring effects of the material can be improved; the difference is 90 The self-controlled rotating cylinder with an angle of 6 degrees is located on the two opposite side axial positions on the inner wall of the cylinder, and there are 4 positive trapezoidal particle boards in the radial direction, which are evenly spaced and distributed in a straight line, which can avoid material agglomeration.
出风侧筒体端盖7内侧中心孔处设有粉料阻挡器,形似迷宫格状,并通过3根不锈钢管固定于出风侧筒体端盖7内侧出风管8管口处,可造成热风流回旋曲折路径的流动方式阻挡热风流中粉料逃逸。There is a powder blocker at the inner center hole of the end cover 7 on the air outlet side, which looks like a labyrinth grid, and is fixed to the outlet pipe 8 on the inner side of the end cover 7 on the air outlet side by 3 stainless steel tubes. The flow mode that causes the hot air flow tortuous path prevents the powder in the hot air flow from escaping.
进、出料装置5设有进、出料门,长方形进、出料门的水平一侧采用双门轴连接于自控旋转筒体6,进、出料门外侧面设有硅胶板具有关闭时的密封功能,此处设有螺栓锁紧装置用于进、出料门关闭时固定进、出料门;进、出料门内推打开后,另有螺杆90°固定进、出料门,便于自控旋转筒体6旋转自动出料。还配有活动进料导板置于进、出料门,便于进料;The inlet and outlet device 5 is provided with inlet and outlet doors, and the horizontal side of the rectangular inlet and outlet doors is connected to the self-control rotating cylinder 6 with double door shafts. The sealing function, there is a bolt locking device to fix the inlet and outlet doors when the inlet and outlet doors are closed; after the inlet and outlet doors are pushed inward to open, there is another screw to fix the inlet and outlet doors at 90°, It is convenient for the self-controlled rotating cylinder 6 to rotate and automatically discharge materials. It is also equipped with a movable feeding guide plate placed on the inlet and outlet doors to facilitate feeding;
出风管8的直径大于进风管3的直径;进风管3及出风管8各一端分别与自控旋转筒体6两侧的进风侧筒体端盖4和出风侧筒体端盖7中心轴承同心、轴向连接,进风管3及出风管8的另两端分别与进风管弯头2-5和旋风分离筒体11进风口之间采用法兰活动套管方式安全性连接。采用这样的连接方式可以保证自控旋转筒体6旋转过程的安全性,确保其他部件不会由于自控旋转筒体6的旋转过程中可能发生的转动不灵活而移位。The diameter of the air outlet pipe 8 is greater than the diameter of the air inlet pipe 3; each end of the air inlet pipe 3 and the air outlet pipe 8 is respectively connected to the air inlet side cylinder end cover 4 and the air outlet side cylinder end on both sides of the self-controlled rotating cylinder 6. The center bearing of the cover 7 is connected concentrically and axially, and the other two ends of the air inlet pipe 3 and the air outlet pipe 8 are respectively connected to the elbows 2-5 of the air inlet pipe and the air inlet of the cyclone separation cylinder 11. The flange movable sleeve method is adopted secure connection. Adopting such a connection method can ensure the safety of the rotation process of the self-controlled rotating cylinder 6 and ensure that other components will not be displaced due to inflexible rotation that may occur during the rotation of the self-controlled rotating cylinder 6 .
4、机架装置4. Rack installation
机架装置(图2)包括自控旋转筒体6下方的机架23,发挥支撑作用,机架23中设有集料板装置22,用于收集出料。The frame device (Fig. 2) includes a frame 23 below the self-controlled rotating cylinder 6 to play a supporting role, and a collecting plate device 22 is arranged in the frame 23 for collecting and discharging materials.
5、旋风分离器5. Cyclone separator
旋风分离器包括旋风分离筒体11及其上部的分离筒中心管道10,下部的锥形筒体12,自控旋转筒体6的出风管8与旋风分离筒体11在圆周相切处联接相通,连接管道处设有电热耦、湿度感受器和温度表。出风风流沿旋风分离筒体内壁高速旋转,可使少量逃逸粉料随旋转风流沿锥形筒体旋转下降而收集,出风风流从旋风分离筒体11上部的分离筒中心管道10排出。The cyclone separator includes a cyclone separation cylinder 11 and its upper separation cylinder central pipe 10, a lower conical cylinder 12, and the air outlet pipe 8 of the self-controlled rotating cylinder 6 is connected to the cyclone separation cylinder 11 at a circumferential tangent. , the connecting pipe is provided with an electric thermocouple, a humidity sensor and a thermometer. The outlet air flow rotates at high speed along the inner wall of the cyclone separation cylinder, so that a small amount of escaped powder can be collected by rotating and descending along the conical cylinder with the rotation air flow.
6、功能控制系统6. Function control system
功能控制系统与鼓风装置1、电加热装置2-1、液体输入泵15、电热耦、湿度感受器、减速机16电联接,并通过微电脑控制器智能化控制上述各部件的工作与停止。电路图如图9。The function control system is electrically connected with the blower device 1, the electric heating device 2-1, the liquid input pump 15, the electric thermocouple, the humidity sensor, and the reducer 16, and intelligently controls the operation and stop of the above-mentioned components through the microcomputer controller. The circuit diagram is shown in Figure 9.
电器控制箱面板按钮显示,红色按钮为培养功能,绿色按钮为干燥功能,分别联接相关功能的微电脑控制仪,智能化控制整个一体机的正常运行,包括:培养温度及湿度控制,工作周期报警、鼓风与滚筒翻搅工作及时控、干燥温度控制,独立控制鼓风,独立控制滚筒翻搅。当电加热装置2-1停止工作,而鼓风装置1仍然继续工作一段时间,其延迟工作的时间可由时控仪任意设定调节。The buttons on the panel of the electrical control box are displayed. The red button is for the cultivation function, and the green button is for the drying function. They are respectively connected to the microcomputer controllers with related functions to intelligently control the normal operation of the whole all-in-one machine, including: cultivation temperature and humidity control, work cycle alarm, Timely control of blast and drum stirring, drying temperature control, independent control of blast, independent control of drum stirring. When the electric heating device 2-1 stops working, and the blower device 1 still continues to work for a period of time, the time for delaying work can be adjusted arbitrarily by the time controller.
固体生态培养、干燥一体机工作条件与参数:Working conditions and parameters of solid ecological cultivation and drying machine:
本申请专利产品自动控制(微电脑控制)有氧发酵培养工作条件:温度35.0±1.0℃(电功率为15kw),湿度75±5.0%,pH 7.2±0.2(在固体培养基配制过程中调控pH),转筒翻搅1min静息5min,培养时间:28小时。在启动热风发生系统培养功能工作的同时,旋转筒体伴随翻搅,以充入丰富的滤过空气氧,为发酵物充分供氧;当培养物料湿度变化时,将通过湿度感受器反馈至功能控制系统,自动启动或关闭液体输入装置调控培养湿度,同时启动风机和转筒工作,以保证物料含水量均匀合适。启动液体输入装置可便捷完成菌种液封闭式均匀接种工作。The patented product of this application is automatically controlled (microcomputer controlled) aerobic fermentation cultivation working conditions: temperature 35.0 ± 1.0 ℃ (electric power is 15kw), humidity 75 ± 5.0%, pH 7.2 ± 0.2 (pH is regulated during the preparation of solid medium), Rotate the drum and stir for 1min and rest for 5min. Incubation time: 28 hours. While starting the cultivation function of the hot air generation system, the rotating cylinder is accompanied by stirring to fill in rich filtered air oxygen to fully supply oxygen for the fermented product; when the humidity of the cultivation material changes, it will be fed back to the function control through the humidity sensor The system automatically starts or closes the liquid input device to regulate the humidity of the culture, and starts the fan and the drum at the same time to ensure that the moisture content of the material is uniform and appropriate. Starting the liquid input device can conveniently complete the closed and uniform inoculation of the strain liquid.
本申请专利产品温敏干燥(微电脑控制)工作条件:温度43±2℃(电功率为30kw),转筒翻搅1min静息3min,在进行物料的温敏干燥过程中,热风发生系统干燥功能持续工作,旋风分离器可有效回收随风流逃逸的物料粉末。The temperature-sensitive drying (microcomputer control) working conditions of the patented product of this application: temperature 43±2°C (electric power 30kw), the drum is stirred for 1 minute and rested for 3 minutes. During the temperature-sensitive drying process of materials, the drying function of the hot air generation system continues Working, the cyclone separator can effectively recover the material powder that escapes with the wind flow.
本发明使用的注意事项:Notes for the use of the present invention:
1)安装可靠接地线。1) Install a reliable ground wire.
2)旋转筒体工作转动时,虽然装有防护板,但严禁靠近转筒与轴筒接触转动处,以防肢体或衣服等卷入造成伤害。2) When the rotating cylinder is working and rotating, although it is equipped with a protective plate, it is strictly forbidden to get close to the contact and rotating part of the rotating cylinder and the shaft cylinder, so as to prevent the limbs or clothes from being involved and causing injury.
3)需要打开旋转筒体进、出料门或端盖前,必须先将机架连杆拉紧钩拉紧固定旋转筒体法兰固定环,然后安全进行相关操作。3) Before opening the inlet and outlet doors or end caps of the rotating cylinder, the tension hook of the connecting rod of the frame must be tightened to fix the flange fixing ring of the rotating cylinder, and then the relevant operations can be carried out safely.
本发明的操作方法:Operation method of the present invention:
调整好本发明设备相关部件,完成进料、接种等相关工作,根据产品生产工艺要求,调整电器箱相关仪器工作参数,操作电器箱功能控制系统相关按钮即可自动工作。Adjust the relevant parts of the equipment of the present invention, complete the related work such as feeding and inoculation, adjust the working parameters of the relevant instruments of the electrical box according to the production process requirements of the product, and operate the relevant buttons of the functional control system of the electrical box to automatically work.
实施例2Example 2
利用固体生态培养、干燥一体机进行解淀粉芽孢杆菌固体生态培养与干燥的相关工艺参数研究:Research on the relevant process parameters of solid ecological cultivation and drying of Bacillus amyloliquefaciens by using the solid ecological cultivation and drying machine:
本专利旨在利用固体生态培养、干燥一体机以益生菌-解淀粉芽孢杆菌为例,进行益生菌固体生态培养、干燥工艺制备技术的研究,以及对其培养、干燥功能的质量评价,提高生产效率,降低生产成本,为解淀粉芽孢杆菌的工厂规模化生产提供相关理论依据和应用参考。This patent aims to use the solid ecological cultivation and drying integrated machine to take probiotics-Bacillus amyloliquefaciens as an example, to conduct research on the preparation technology of probiotic solid ecological cultivation and drying process, and to evaluate the quality of its cultivation and drying functions, so as to improve production Improve efficiency, reduce production costs, and provide relevant theoretical basis and application reference for the large-scale production of Bacillus amyloliquefaciens.
1材料与方法1 Materials and methods
1.1菌种1.1 Strains
菌种是购自中国工业微生物菌种保藏管理中心的解淀粉芽孢杆菌Bacillusamyloliquefaciens CICC 20606。The strain was Bacillus amyloliquefaciens CICC 20606 purchased from the China Industrial Microorganism Culture Collection Management Center.
1.2培养基1.2 Medium
液体菌种培养基:蛋白胨5g、葡萄糖5g、酵母膏5g、K2HPO44g、蒸馏水1L、pH调至7.2。常规方法制备菌种液。Liquid culture medium: peptone 5g, glucose 5g, yeast extract 5g, K 2 HPO 4 4g, distilled water 1L, pH adjusted to 7.2. The seed solution was prepared by conventional methods.
固体发酵培养基原料及试剂:玉米25%、麸皮30%、豆粕15%、稻壳26.5%、CaH2PO43%、NaCl 0.23%、MgSO40.15%、MnSO40.01%、K2HPO40.11%。用于固体生态培养·干燥一体机的培养。Solid fermentation medium raw materials and reagents: corn 25%, bran 30%, soybean meal 15%, rice husk 26.5%, CaH 2 PO 4 3%, NaCl 0.23%, MgSO 4 0.15%, MnSO 4 0.01%, K 2 HPO 4 0.11%. It is used for the cultivation of solid ecological cultivation and drying machine.
1.3采用固体生态培养·干燥一体机培养、干燥试验1.3 Cultivation and drying test using solid ecological cultivation and drying machine
1.3.1培养试验1.3.1 Culture test
根据固体发酵培养配方配制1000kg固体发酵培养基原料,按料水比1:1.3加入无菌纯水,以单手握紧料成团,轻抖即散为宜,用3mol/L的NaOH调节培养基初始pH至7.2,培养基消毒、熟化采用0.15Mpa,121℃,1h。降温后无菌操作转移进灭菌消毒的固体生态培养·干燥一体机中,以15%的菌种液接种量封闭式接种,菌种液活菌数达到120×108CFU/g以上,自动控制(微电脑控制)有氧发酵培养工作条件:温度35.0±1.0℃,湿度75±3.0%,初始pH 7.2±0.2(在固体培养基配制过程中调控pH),转筒翻搅1min静息5min,培养时间:28小时。1.3.2干燥试验Prepare 1000kg of solid fermentation medium raw materials according to the solid fermentation culture formula, add sterile pure water according to the material-to-water ratio of 1:1.3, hold the material tightly with one hand to form a ball, shake lightly to disperse, and adjust the culture with 3mol/L NaOH The initial pH of the base is 7.2, and the culture medium is sterilized and matured at 0.15Mpa at 121°C for 1h. After cooling down, the aseptic operation is transferred to the sterilized solid ecological cultivation and drying machine, and the closed inoculation is performed with 15% of the inoculation amount of the strain liquid. Controlled (microcomputer controlled) working conditions for aerobic fermentation culture: temperature 35.0±1.0°C, humidity 75±3.0%, initial pH 7.2±0.2 (pH is regulated during the preparation of solid medium), tumbled for 1min and rested for 5min, Incubation time: 28 hours. 1.3.2 Drying test
发酵培养结束后,温敏干燥(微电脑控制)工作条件:温度43±2℃,转筒翻搅1min,静息3min,物料干燥至含水量≤10%,干燥过程结束。收集产物并粉碎过筛(按国标双层筛分法,过60-80目,80目筛上物≤10%),检测活菌菌落数。After the fermentation and cultivation, the temperature-sensitive drying (microcomputer control) working conditions: temperature 43±2°C, drum stirring for 1 min, resting for 3 min, the material is dried to a moisture content of ≤10%, and the drying process is over. The product was collected and crushed and sieved (according to the national standard double-layer sieving method, 60-80 mesh, 80 mesh sieve ≤ 10%), and the number of viable bacterial colonies was detected.
1.4检测方法1.4 Detection method
1.4.1活菌计数1.4.1 Viable bacteria count
参照国标(GB/T 26428-2010)进行测定。Refer to the national standard (GB/T 26428-2010) for determination.
1.4.2芽孢计数1.4.2 Spore count
参照国标(GB/T 26428-2010)进行测定。Refer to the national standard (GB/T 26428-2010) for determination.
1.5数据分析1.5 Data Analysis
所有数据均以Excel建立数据库,以表示。All data are built in Excel database, with express.
2实验结果与分析2 Experimental results and analysis
2.1解淀粉芽孢杆菌固体生态培养2.1 Solid ecological culture of Bacillus amyloliquefaciens
2.1.1pH动态变化(图10):2.1.1 pH dynamic changes (Figure 10):
三批固体培养试验的pH平均值在6.4±0.06~7.1±0.09之间,初始4h后平均pH的变化范围在6.6±0.20。在发酵过程中,前16h pH下降比较明显,16h后,pH值呈略微上升趋势。The average pH values of the three batches of solid culture tests were between 6.4±0.06 and 7.1±0.09, and the average pH range after the initial 4h was 6.6±0.20. During the fermentation process, the pH decreased significantly in the first 16 hours, and after 16 hours, the pH value showed a slight upward trend.
2.1.2温度动态变化(图11)2.1.2 Dynamic changes in temperature (Figure 11)
3批固体生态培养试验的温度控制在35.0±1.0℃,从图11可见,三次连续培养温度的平均值在36.47±0.088~37.20±0.115之间,且温度变化不大而保持稳定。The temperature of the three batches of solid ecological cultivation experiments was controlled at 35.0±1.0°C. It can be seen from Figure 11 that the average value of the three continuous cultivation temperatures was between 36.47±0.088 and 37.20±0.115, and the temperature remained stable with little change.
2.1.3湿度动态变化(图12)2.1.3 Humidity dynamic changes (Figure 12)
3批固体生态培养试验的湿度控制在75±5.0%,从图12可见,三次连续培养湿度的平均值在83.33±0.333%~86±0.577%之间变化,其湿度变化范围不大可保持培养湿度的相对稳定。The humidity of the 3 batches of solid ecological culture tests was controlled at 75±5.0%. It can be seen from Figure 12 that the average value of the humidity in the three consecutive cultures varied between 83.33±0.333% and 86±0.577%. Humidity is relatively stable.
2.1.4活菌数动态变化(图13)2.1.4 Dynamic change of viable bacteria count (Figure 13)
3批固体生态培养试验过程中,活菌数变化比较有规律,在发酵刚开始0~4h活菌数比较低,可能是由于解淀粉芽孢杆菌接种至固体培养基后,对新环境有一个短暂的适应过程,活力较低。4~16h菌体活力逐渐增强,16h后解淀粉芽孢杆菌进行迅速的生长繁殖,菌体数量增长很快。16~28h活菌数呈快速上升趋势,在此过程中,芽孢不断形成。During the three batches of solid ecological culture tests, the number of viable bacteria changed regularly, and the number of viable bacteria was relatively low at the beginning of fermentation 0 to 4 hours, which may be due to the short-term effect of Bacillus amyloliquefaciens on the new environment after being inoculated into the solid medium. The adaptation process, the vitality is low. After 4-16 hours, the vigor of the bacteria gradually increased, and after 16 hours, Bacillus amyloliquefaciens grew rapidly, and the number of bacteria increased rapidly. From 16 to 28 hours, the number of viable bacteria increased rapidly, and during this process, spores were continuously formed.
2.2解淀粉芽孢杆菌固体生态培养物的干燥(图14)2.2 Drying of Bacillus amyloliquefaciens solid ecological culture (Figure 14)
3批固体生态培养物经6h干燥后,含水量分别为9.0%,8.3%,8.6%,平均为8.63%±0.203,符合国标对固体发酵物干燥后含水量的要求。After drying for 6 hours, the three batches of solid ecological cultures had water contents of 9.0%, 8.3%, and 8.6%, respectively, with an average of 8.63%±0.203, which met the national standard for water content of solid fermented products after drying.
2.3解淀粉芽孢杆菌固体生态培养物干燥后芽孢率(图15)2.3 The spore rate of Bacillus amyloliquefaciens solid ecological culture after drying (Figure 15)
3批固体生态培养物经6h干燥后,芽孢率分别为78%,80%,85%,平均为81±2.1%,表明本申请专利的固体生态培养·干燥一体机对总体的固体生态培养和干燥过程的控制是很好的。After the 3 batches of solid ecological cultures were dried for 6 hours, the spore rates were 78%, 80%, and 85%, respectively, with an average of 81±2.1%, indicating that the solid ecological cultivation and drying integrated machine of the patent application has a great impact on the overall solid ecological cultivation and The control of the drying process is very good.
3讨论3 Discussion
3.1精确控制发酵条件对发酵效果的评价3.1 Evaluation of fermentation effect by precise control of fermentation conditions
本研究采用自行研制的固体生态培养·干燥一体机对解淀粉芽孢杆菌固体发酵过程进行研究,由于从生态仿生角度,智能化和合理性控制相关发酵参数,从而使整个发酵过程具有稳定性好、效率高的特点。In this study, the self-developed solid ecological cultivation and drying machine was used to study the solid fermentation process of Bacillus amyloliquefaciens. From the perspective of ecological bionics, the relevant fermentation parameters are intelligently and rationally controlled, so that the whole fermentation process has good stability and Features of high efficiency.
3.2控制pH值相对稳定对固体发酵效果的影响3.2 The effect of controlling the relatively stable pH value on the solid fermentation effect
合适的pH水平是益生菌生长和代谢产物合成的非常重要的条件参数,也是代谢活动的综合反映。本研究中发酵底物的温度和湿度在相对稳定的条件下,表现为发酵前期0-16h阶段,pH值呈逐渐下降趋势,反映出益生菌活性呈现快速增强状态,代谢的酸性产物逐渐增加;然而发酵后期16-28h阶段,pH值呈逐渐回升趋势,这由于本发明固体生态培养·干燥一体机能够旋转翻搅培养物料并充分供给温湿度适宜的空气氧,有效缓冲培养过程中逐渐下降的pH值,而且总体pH值的波动控制在较小的适宜范围内,较好地维持了整体发酵培养过程pH值的相对稳定,同时活菌数量呈现出快速增长状况。可能是由于培养过程中供氧充分,在培养后期培养物料溶氧增大所致The appropriate pH level is a very important condition parameter for the growth of probiotics and the synthesis of metabolites, and it is also a comprehensive reflection of metabolic activities. In this study, the temperature and humidity of the fermentation substrate were relatively stable, showing a gradual decline in the pH value at the stage of 0-16h in the early stage of fermentation, reflecting the rapid increase in the activity of probiotics and the gradual increase in the acidic products of metabolism; However, at the 16-28h stage in the late stage of fermentation, the pH value tends to rise gradually. This is because the solid ecological cultivation and drying all-in-one machine of the present invention can rotate and stir the cultivation materials and fully supply air and oxygen with suitable temperature and humidity, which can effectively buffer the gradually falling pH in the cultivation process. The pH value, and the fluctuation of the overall pH value is controlled within a small suitable range, which better maintains the relative stability of the pH value in the overall fermentation and cultivation process, and at the same time, the number of viable bacteria shows a rapid increase. It may be due to the sufficient oxygen supply during the cultivation process, and the increase of dissolved oxygen in the culture material in the later stage of cultivation
3.3温度控制对固体发酵效果的的影响3.3 Effect of temperature control on solid fermentation effect
本发明的固体生态培养·干燥一体机采用微电脑自动控制培养温度为35℃±1.0℃,,整个发酵过程中温控稳定,这有助于保持较高的酶活性状态,符合解淀粉芽孢杆菌生长繁殖最适生长温度范围的需要。更重要的是由于筒体的旋转翻搅,保证了全部培养物料的发酵温度均匀一致和充分供氧,符合解淀粉芽孢杆菌生长繁殖的最适生长温度范围的需要并有效提高发酵培养质量。The solid ecological cultivation and drying all-in-one machine of the present invention uses a microcomputer to automatically control the cultivation temperature to 35°C±1.0°C, and the temperature control is stable throughout the fermentation process, which helps to maintain a high enzyme activity state, which is in line with the growth of Bacillus amyloliquefaciens The need for optimum growth temperature range for propagation. More importantly, due to the rotation and stirring of the cylinder, the fermentation temperature of all culture materials is guaranteed to be uniform and the oxygen supply is sufficient, which meets the needs of the optimum growth temperature range for the growth and reproduction of Bacillus amyloliquefaciens and effectively improves the quality of fermentation culture.
3.4湿度控制对固体发酵效果的影响3.4 Effect of humidity control on solid fermentation effect
本研究设备通过智能温湿度仪自动控制培养湿度的稳定,此外也控制培养温度的稳定。当培养物料湿度变化时,将自动启动或关闭湿度自控及菌液接种输入系统的喷射液体装置,调控培养湿度,同时启动风机和转筒工作,以保证物料含水量均匀。本研究中固体发酵湿度控制为75±5.0%,结果表明能满足解淀粉芽孢杆菌生长繁殖所需的适宜湿度范围。是由于液体输入装置均匀、柔和的物料自动加湿功能所发挥的作用,符合解淀粉芽孢杆菌生长繁殖的最适生长湿度范围的需要。所设定的湿度值偏低于实际测定值,是由于本发明固体生态培养·干燥一体机的湿度感受器设置在自动旋转筒体的出风口,液体输入装置的自动加湿功能与湿度感受器的反馈信息在时间上有一延迟,会使湿度值提高,此外,在一定的温度条件下,湿度值也会提高。因此,考虑到这些因素的影响,可用来指导湿度的设定。The research equipment automatically controls the stability of the culture humidity through the intelligent temperature and humidity meter, and also controls the stability of the culture temperature. When the humidity of the culture material changes, it will automatically start or shut down the spray liquid device of the humidity automatic control and bacterial liquid inoculation input system to control the culture humidity, and start the fan and drum at the same time to ensure that the water content of the material is uniform. In this study, the humidity of solid fermentation was controlled at 75±5.0%, and the results showed that it could meet the suitable range of humidity required for the growth and reproduction of Bacillus amyloliquefaciens. It is due to the uniform and gentle material automatic humidification function of the liquid input device, which meets the needs of the optimum growth humidity range for the growth and reproduction of Bacillus amyloliquefaciens. The set humidity value is slightly lower than the actual measured value, because the humidity sensor of the solid ecological cultivation and drying all-in-one machine of the present invention is arranged at the air outlet of the automatic rotating cylinder, the automatic humidification function of the liquid input device and the feedback information of the humidity sensor There is a delay in time, which will increase the humidity value. In addition, under certain temperature conditions, the humidity value will also increase. Therefore, considering the influence of these factors, it can be used to guide the setting of humidity.
3.5固体生态培养·干燥一体机液体菌种封闭式接种方式对发酵效率的影响3.5 The influence of the closed inoculation method of liquid strains on the fermentation efficiency of the solid ecological cultivation and drying all-in-one machine
固体生态培养·干燥一体机可在封闭无菌状态下,将菌种液通过接种系统、风力分配器的协同工作,将菌种液均匀混合接种于培养物料。其特点表现为:安全、快速、均匀、简便,尤其在益生菌培养初期抗杂菌感染效果较为理想。The solid ecological cultivation and drying all-in-one machine can inoculate the culture material evenly by mixing the seed liquid through the inoculation system and the wind distributor in a closed and sterile state. Its characteristics are as follows: safe, fast, uniform, and simple, especially in the initial stage of probiotic culture, the anti-microbial infection effect is relatively ideal.
3.6培养过程中的活菌数动态变化特点3.6 Characteristics of dynamic changes in the number of viable bacteria during the cultivation process
在本研究的特定培养条件下,随着整个发酵进程,解淀粉芽孢杆菌活菌数呈现出持续升高的动态性变化。发酵培养的初期属于生长延滞期,其活菌数量上升不明显,但是培养物料的pH值呈逐渐下降趋势,代谢的酸性产物逐渐增加,反映出益生菌活性呈现快速增强状态,此后活菌数呈大幅度上升趋势,属于细菌的生长指数期,代谢旺盛。研究结果反映出培养过程中相关培养条件的控制是较为合适的。Under the specific culture conditions of this study, the number of viable Bacillus amyloliquefaciens showed a dynamic change that continued to increase throughout the fermentation process. The initial stage of fermentation culture belongs to the growth lag period, and the number of viable bacteria does not increase significantly, but the pH value of the culture material gradually decreases, and the acidic products of metabolism gradually increase, reflecting the rapid increase in the activity of probiotics. A sharp upward trend belongs to the growth index period of bacteria, and the metabolism is vigorous. The research results reflect that the control of the relevant culture conditions in the culture process is more appropriate.
3.7培养物干燥后含水率和芽孢率变化特点3.7 Change characteristics of moisture content and spore rate of culture after drying
培养物料经干燥后解淀粉芽孢杆菌粉的含水率平均为8.633%±0.203,芽孢率平均为81%±2.081,符合国家相关产品质量控制要求。本研究采用热风温控系统,自动翻搅系统,旋风分离回收装置同时工作的状态下,保持43±2℃的快速低温风流干燥条件下,,结果表明可大幅度提高培养物料的表面积,高效率去除培养物料中的水分,有效保持益生菌数量和活性,达到快速温敏干燥但能维持菌体活性的理想效果。After the culture material is dried, the average water content of the Bacillus amyloliquefaciens powder is 8.633% ± 0.203, and the average spore rate is 81% ± 2.081, which meets the relevant national product quality control requirements. In this study, the hot air temperature control system, automatic stirring system, and cyclone separation and recovery device are used at the same time, and the rapid low-temperature air drying condition of 43±2°C is maintained. The results show that the surface area of the culture material can be greatly increased, and the efficiency is high. Remove the moisture in the culture material, effectively maintain the quantity and activity of probiotics, and achieve the ideal effect of rapid temperature-sensitive drying but maintaining the activity of the bacteria.
3.8固体生态培养·干燥一体机工作效果评价3.8 Evaluation of the working effect of the solid ecological cultivation and drying machine
通过本申请专利固体生态培养·干燥一体机对解淀粉芽孢杆菌固体生态培养和干燥的工艺流程进行研究,从仿生学角度研制相关结构,由自动翻搅系统调控转筒工作,能够自动化控制有氧生物发酵底物的搅拌强度,并使物料充分获氧,发酵均匀。温、湿度控制系统通过风力分配器,可使搅拌均匀的物料处于温、湿度适宜的仿生态培养环境中,确保益生菌群的高效培育。The solid ecological cultivation and drying process of Bacillus amyloliquefaciens is studied through the patented solid ecological cultivation and drying machine of this application, and the relevant structure is developed from the perspective of bionics. The automatic stirring system regulates the work of the drum, which can automatically control aerobic Stirring intensity of the biological fermentation substrate, and make the material fully obtain oxygen and ferment evenly. The temperature and humidity control system, through the wind distributor, can make the uniformly stirred materials in an ecological-like cultivation environment with suitable temperature and humidity, so as to ensure the efficient cultivation of probiotics.
通过菌种液接种输入系统可安全封闭式定量接种菌种液,并能有效地达到较好的封闭状态,使杂菌率控制在1%以下。The seed liquid inoculation input system can safely and quantitatively inoculate the seed liquid in a closed manner, and can effectively achieve a better closed state, so that the rate of miscellaneous bacteria is controlled below 1%.
本研究中,固体生态培养·干燥一体机自动控制的有氧生物发酵培养工作条件设置为:温度35.0±1.0℃,湿度75±5.0%,初始pH 7.2±0.2(在固体培养基配制过程中调控pH),转筒翻搅1min静息5min,培养时间:28小时,最终发酵物料中含菌量平均达到203.333±4.410×108CFU/g;自动控制温敏干燥工作条件设置为:温度43±2℃,转筒翻搅1min静息3min。芽孢率平均为81±2.081%,含水量平均为8.633±0.203%。在进行物料的温敏干燥过程中,干燥热风温控系统持续工作,旋风分离器可有效回收随风流逃逸的物料粉末。与液体发酵工艺相比,显著性提高了有氧生物发酵产量,大幅度降低了生产成本,能完全满足需氧益生菌工厂规模化生产的要求。In this study, the working conditions of aerobic fermentation culture automatically controlled by the solid ecological cultivation and drying machine were set as follows: temperature 35.0±1.0°C, humidity 75±5.0%, initial pH 7.2±0.2 (adjusted during the preparation of solid medium pH), the tumbler was stirred for 1min and rested for 5min, the cultivation time: 28 hours, and the average bacterial content in the final fermentation material reached 203.333±4.410×10 8 CFU/g; the working condition of automatic control temperature-sensitive drying was set as: temperature 43± At 2°C, the drum was stirred for 1 min and rested for 3 min. The average spore rate was 81±2.081%, and the average water content was 8.633±0.203%. During the temperature-sensitive drying process of materials, the drying hot air temperature control system continues to work, and the cyclone separator can effectively recover the material powder that escapes with the air flow. Compared with the liquid fermentation process, the production of aerobic biological fermentation is significantly improved, the production cost is greatly reduced, and the requirements for large-scale production of aerobic probiotic factories can be fully met.
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