CN103463616B - A kind of chitosan and tripeptides composition and method of making the same and application - Google Patents
A kind of chitosan and tripeptides composition and method of making the same and application Download PDFInfo
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Abstract
本发明公开了一种壳聚糖与甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物及其制备方法和应用，其制备方法为：配制0.01wt%-20wt%的甘氨酸-L-半胱氨酸-高牛磺酸三肽的水溶液；配制壳聚糖凝胶：含0.01-10wt%羧甲基壳聚糖，搅拌充分溶胀成凝胶，加入甘氨酸-L-半胱氨酸-高牛磺酸三肽的水溶液，搅拌均匀，得到壳聚糖与甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物凝胶；采用该方法制备的组合物，具备了比两者单独使用更好的抗放射性皮炎及促创口愈合的作用。
The invention discloses a chitosan and glycine-L-cysteine-homotaurine tripeptide composition and its preparation method and application. The preparation method is: preparing 0.01wt%-20wt% glycine-L -Aqueous solution of cysteine-homotaurine tripeptide; preparation of chitosan gel: containing 0.01-10wt% carboxymethyl chitosan, stirring to fully swell into a gel, adding glycine-L-cysteine - the aqueous solution of homotaurine tripeptide, stir evenly, obtain chitosan and glycine-L-cysteine-homotaurine tripeptide composition gel; Those who use it alone have a better effect on anti-radiation dermatitis and promoting wound healing.
Description
技术领域technical field
本发明涉及壳聚糖与甘氨酸-L-半胱氨酸-高牛磺酸三肽的组合物及其制备方法和应用，旨在通过组合，协同提高两种化合物抗放射性皮炎、促进创口愈合的能力，属于医学材料领域。The invention relates to a composition of chitosan and glycine-L-cysteine-homotaurine tripeptide and its preparation method and application, aiming at synergistically improving the anti-radiation dermatitis and promoting wound healing effects of the two compounds through combination. Ability, belonging to the field of medical materials.
背景技术Background technique
甲壳素是从虾、蟹等甲壳类动物的甲壳以及真菌的细胞壁中提取出来的一种天然多糖，也是自然界仅次于纤维素的第二大生物资源。壳聚糖是甲壳素的脱乙酰化衍生物，具有良好的生物相容性、生物可降解性、天然无毒性和多种生物活性。近年来，壳聚糖作为一种天然医用生物敷料，因其抗菌性和抗炎作用，在创口护理方面应用受到人们的广泛关注。Chitin is a natural polysaccharide extracted from the shells of crustaceans such as shrimps and crabs and the cell walls of fungi. It is also the second largest biological resource in nature after cellulose. Chitosan is a deacetylated derivative of chitin, which has good biocompatibility, biodegradability, natural non-toxicity and various biological activities. In recent years, chitosan, as a natural medical biological dressing, has attracted widespread attention in wound care because of its antibacterial and anti-inflammatory effects.
中国专利申请号201210534121.5公开了甘氨酸-L-半胱氨酸-高牛磺酸三肽的结构及其制备方法，并详细描述了其在制备治疗脑卒中药物中的应用，甘氨酸-L-半胱氨酸-高牛磺酸三肽制备的抗脑卒中的药物具有较好的疗效，通过动物实验表明甘氨酸-L-半胱氨酸-高牛磺酸三肽可改善大鼠的行为学，起到治疗脑卒中的作用。Chinese Patent Application No. 201210534121.5 discloses the structure and preparation method of glycine-L-cysteine-homotaurine tripeptide, and describes its application in the preparation of stroke medicine in detail. Glycine-L-cysteine The anti-stroke drug prepared by amino acid-homotaurine tripeptide has a good curative effect. Animal experiments show that glycine-L-cysteine-homotaurine tripeptide can improve the behavior of rats and play a role to the treatment of stroke.
发明内容Contents of the invention
本发明的目的是提供了壳聚糖与甘氨酸-L-半胱氨酸-高牛磺酸三肽的组合物及其制备方法和应用，采用该方法制备的组合物，具备了比两者单独使用更好的抗放射性皮炎及促创口愈合的作用。The object of the present invention is to provide the composition of chitosan and glycine-L-cysteine-homotaurine tripeptide and its preparation method and application, adopt the composition prepared by this method, have possessed than both alone Use better anti-radiation dermatitis and promote wound healing.
本发明提供的技术方案是：The technical scheme provided by the invention is:
壳聚糖与甘氨酸-L-半胱氨酸-高牛磺酸三肽的组合其制备方法为：配制0.01%-20%的甘氨酸-L-半胱氨酸-高牛磺酸三肽的水溶液；配制壳聚糖凝胶：含0.01-10%羧甲基壳聚糖，搅拌充分溶胀成凝胶，加入甘氨酸-L-半胱氨酸-高牛磺酸三肽的水溶液，搅拌均匀，得到壳聚糖与甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物凝胶。Combination of chitosan and glycine-L-cysteine-homotaurine tripeptide The preparation method is: prepare 0.01%-20% aqueous solution of glycine-L-cysteine-homotaurine tripeptide ; Preparation of chitosan gel: containing 0.01-10% carboxymethyl chitosan, fully swollen into a gel by stirring, adding glycine-L-cysteine-homotaurine tripeptide aqueous solution, stirring evenly to obtain Chitosan and glycine-L-cysteine-homotaurine tripeptide composition gel.
壳聚糖与甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物具备抗放射性皮炎和促进伤口愈合作用，其作用优于单纯使用壳聚糖、单纯使用甘氨酸-L-半胱氨酸-高牛磺酸三肽的作用，实验结果见表1，图1。The composition of chitosan and glycine-L-cysteine-homotaurine tripeptide has the effect of anti-radiation dermatitis and promoting wound healing, and its effect is better than that of chitosan alone and glycine-L-cysteine alone The effect of acid-homotaurine tripeptide, the experimental results are shown in Table 1, Figure 1.
本发明采用上述的技术方案，其优点在于：保留壳聚糖对皮损的抗菌、抗炎作用，同时复配甘氨酸-L-半胱氨酸-高牛磺酸三肽的促细胞增殖作用，增强促伤口愈合的效果。The present invention adopts the above-mentioned technical scheme, and its advantage is: retain the antibacterial and anti-inflammatory effects of chitosan on skin lesions, and simultaneously compound the effect of promoting cell proliferation of glycine-L-cysteine-homotaurine tripeptide, Enhance the effect of promoting wound healing.
壳聚糖与甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物具备抗感染作用，作用优于单纯使用甘氨酸-L-半胱氨酸-高牛磺酸三肽的作用，实验结果见表2；具有促伤口愈合作用，其作用优于单纯使用壳聚糖、单纯使用甘氨酸-L-半胱氨酸-高牛磺酸三肽的作用，实验结果见表3。The composition of chitosan and glycine-L-cysteine-homotaurine tripeptide has anti-infection effect, which is better than that of glycine-L-cysteine-homotaurine tripeptide alone. Experiment The results are shown in Table 2; it has the effect of promoting wound healing, and its effect is better than that of simply using chitosan and glycine-L-cysteine-homotaurine tripeptide. The experimental results are shown in Table 3.
本发明利用壳聚糖的杀菌性、抗炎性及可以成膜后的阻菌性，在皮损部位和创口表面形成护理膜，利用甘氨酸-L-半胱氨酸-高牛磺酸三肽的抗氧化性，渗入组织内，促进细胞增殖、加速创口愈合。由于壳聚糖成膜后，可形成局部半封闭环境，并增强皮肤的水合作用，可提高甘氨酸-L-半胱氨酸-高牛磺酸三肽的透皮吸收性。The present invention utilizes chitosan's bactericidal, anti-inflammatory and film-forming antibacterial properties to form a nursing film on the skin lesion and wound surface, and utilizes glycine-L-cysteine-homotaurine tripeptide Antioxidant, penetrates into tissues, promotes cell proliferation, and accelerates wound healing. After chitosan forms a film, it can form a local semi-closed environment and enhance the hydration of the skin, which can improve the transdermal absorption of glycine-L-cysteine-homotaurine tripeptide.
附图说明Description of drawings
图1壳聚糖与甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物、壳聚糖凝胶、甘氨酸-L-半胱氨酸-高牛磺酸三肽、生理盐水治疗放射性皮炎的作用比较。Fig. 1 Chitosan and glycine-L-cysteine-homotaurine tripeptide composition, chitosan gel, glycine-L-cysteine-homotaurine tripeptide, normal saline to treat radioactivity Comparison of the effects of dermatitis.
具体实施方式Detailed ways
以下结合具体实施例，对本发明进行详细说明。The present invention will be described in detail below in conjunction with specific embodiments.
实施例1：Example 1:
将20g甘氨酸-L-半胱氨酸-高牛磺酸三肽溶解在50mL水溶液中，配制得40%甘氨酸-L-半胱氨酸-高牛磺酸三肽水溶液。将0.1g羧甲基壳聚糖加50ml水溶胀，过夜消泡，配制得0.2%壳聚糖凝胶。将上述两种溶液等量混合，搅匀，制备得0.1%壳聚糖+20%甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物。Dissolve 20 g of glycine-L-cysteine-homotaurine tripeptide in 50 mL of aqueous solution to prepare a 40% aqueous solution of glycine-L-cysteine-homotaurine tripeptide. Add 0.1g of carboxymethyl chitosan to 50ml of water to swell and defoam overnight to prepare 0.2% chitosan gel. The above two solutions were mixed in equal amounts and stirred evenly to prepare a 0.1% chitosan+20% glycine-L-cysteine-homotaurine tripeptide composition.
实施例2:Example 2:
将0.01g甘氨酸-L-半胱氨酸-高牛磺酸三肽溶解在50mL水溶液中，配制得0.02%甘氨酸-L-半胱氨酸-高牛磺酸三肽水溶液。将5g羧甲基壳聚糖加50ml水溶胀，过夜消泡，配制得10%壳聚糖凝胶。将上述两种溶液等量混合，搅匀，制备得10%壳聚糖+0.01%甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物。Dissolve 0.01g glycine-L-cysteine-homotaurine tripeptide in 50mL aqueous solution to prepare 0.02% glycine-L-cysteine-homotaurine tripeptide aqueous solution. Add 50ml of water to swell 5g of carboxymethyl chitosan and defoam overnight to prepare 10% chitosan gel. The above two solutions were mixed in equal amounts and stirred evenly to prepare a 10% chitosan+0.01% glycine-L-cysteine-homotaurine tripeptide composition.
实施例3:采用1%壳聚糖+5%甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物，建立大鼠放射性皮炎模型，探讨组合物抗放射性皮炎的作用。Embodiment 3: adopt 1% chitosan+5% glycine-L-cysteine-homotaurine tripeptide composition, establish the rat radiation dermatitis model, explore the effect of composition anti-radiation dermatitis.
材料方法material method
（一）试验动物(1) Experimental animals
SD鼠，雌雄兼用，由武汉大学动物试验中心提供。SD mice, both male and female, were provided by the Animal Experiment Center of Wuhan University.
（二）试验材料(2) Test materials
试剂：羧甲基壳聚糖(药用级，浙江金壳生物化学有限公司)，批号：D061226300；甘氨酸-L-半胱氨酸-高牛磺酸三肽，自己合成。Reagent: carboxymethyl chitosan (pharmaceutical grade, Zhejiang Jinke Biochemical Co., Ltd.), batch number: D061226300; glycine-L-cysteine-homotaurine tripeptide, synthesized by ourselves.
（三）试验方法(3) Test method
造模方法：采用6Mev电子线，吸收剂量为60Gy，剂量率为600cGy/分，一次照射对大鼠裸露的右后大腿，产生10%II度放射性皮炎。Modeling method: 6 Mev electron beams were used, the absorbed dose was 60Gy, and the dose rate was 600cGy/min. Once irradiated to the bare right rear thigh of the rats, 10% of them had grade II radiation dermatitis.
分组及给药：动物24只，随机分为4组，每组6只。模型组；模型+壳聚糖凝胶治疗组；模型组+甘氨酸-L-半胱氨酸-高牛磺酸三肽组；模型+壳聚糖+甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物组；于出现急性放射性皮炎后，模型组每天于照射部位喷涂生理盐水，一天二次；给药物喷涂相应药物，于照射部位，一天二次。于给药后4周结束实验，照像并用图像软件计算皮炎面积。Grouping and administration: 24 animals were randomly divided into 4 groups, 6 in each group. Model group; model + chitosan gel treatment group; model group + glycine-L-cysteine-homotaurine tripeptide group; model + chitosan + glycine-L-cysteine-homotaurine Sulfonic acid tripeptide composition group; after acute radiation dermatitis, the model group sprayed physiological saline on the irradiated part every day, twice a day; sprayed corresponding drugs on the irradiated part, twice a day. The experiment was finished 4 weeks after administration, and the dermatitis area was calculated by taking pictures and using image software.
统计学处理：采用SPSS10.0如见进行统计分析。计量资料以（χ±S）表示，结果进行t检验；计数资料采用卡方检验。P<0.05为差异显著。Statistical analysis: SPSS10.0 is used for statistical analysis. The measurement data were expressed as (χ±S), and the results were carried out by t test; the enumeration data were carried out by chi-square test. P<0.05 means significant difference.
实验结果Experimental results
治疗放射性皮炎的作用研究Study on the effect of treating radiation dermatitis
结果可见，壳聚糖凝胶、甘氨酸-L-半胱氨酸-高牛磺酸三肽水溶液、壳聚糖与甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物对放射性皮炎均有显著的治疗作用，且治疗作用以壳聚糖与甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物效果最好，结果见表1、图1。如图1所示，经6Mev电子线，吸收剂量为60Gy的照射，14天后，采用相应药物进行治疗4周，结果可见，生理盐水组的平均红肿面积为：0.94cm2、单独壳聚糖凝胶组的平均红肿面积为：0.49cm2、单独甘氨酸-L-半胱氨酸-高牛磺酸三肽水溶液的平均红肿面积为：0.45cm2、壳聚糖与甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物的平均红肿面积为：0.08cm2。表明组合物具备抗放射性皮炎作用，其作用优于单纯使用壳聚糖、单纯使用甘氨酸-L-半胱氨酸-高牛磺酸三肽的作用The results show that chitosan gel, glycine-L-cysteine-homotaurine tripeptide aqueous solution, chitosan and glycine-L-cysteine-homotaurine tripeptide composition have no effect on radiation dermatitis All have significant therapeutic effect, and the therapeutic effect is best with chitosan and glycine-L-cysteine-homotaurine tripeptide composition, the results are shown in Table 1 and Figure 1. As shown in Figure 1, 6 Mev electron beams were irradiated with an absorbed dose of 60Gy. After 14 days, corresponding drugs were used for 4 weeks of treatment. The results showed that the average red and swollen area of the normal saline group was 0.94cm 2 . The average red and swollen area of the glue group is: 0.49cm 2 , the average red and swollen area of the glycine-L-cysteine-homotaurine tripeptide aqueous solution alone is 0.45cm 2 , the chitosan and glycine-L-cysteine The average area of redness and swelling of the acid-homotaurine tripeptide composition is: 0.08cm 2 . It shows that the composition has anti-radiation dermatitis effect, and its effect is better than that of using chitosan alone, glycine-L-cysteine-homotaurine tripeptide alone
表1.壳聚糖凝胶、三肽水溶液、壳聚糖与三肽组合物治疗放射性皮炎的作用Table 1. The effect of chitosan gel, tripeptide aqueous solution, chitosan and tripeptide composition treatment radiation dermatitis
（χ±S）(χ±S)
实施例4:采用2%壳聚糖+2%甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物，建立家兔创伤模型、小鼠感染模型，探讨组合物对伤口的保护作用。Embodiment 4: adopt 2% chitosan+2% glycine-L-cysteine-homotaurine tripeptide composition, set up rabbit trauma model, mouse infection model, explore composition to the protective effect of wound .
材料方法material method
（一）试验动物(1) Experimental animals
日本大耳白兔，雌雄兼用，由武汉大学动物试验中心提供；昆明小鼠，雌雄兼用，由武汉大学动物试验中心提供。Japanese big-eared white rabbits, male and female, provided by the Animal Experiment Center of Wuhan University; Kunming mice, male and female, provided by the Animal Experiment Center of Wuhan University.
（二）试验材料(2) Test materials
试剂：羧甲基壳聚糖(药用级，浙江金壳生物化学有限公司)，批号：D061226300；甘氨酸-L-半胱氨酸-高牛磺酸三肽（食品级，广州久恒贸易有限公司），批号：20110302。Reagent: carboxymethyl chitosan (pharmaceutical grade, Zhejiang Jinke Biochemical Co., Ltd.), batch number: D061226300; glycine-L-cysteine-homotaurine tripeptide (food grade, Guangzhou Jiuheng Trading Co., Ltd. ), batch number: 20110302.
药品：青霉素钠，国药集团国瑞药业有限公司提供，批号20070518；龙胆紫由国药集团化学试剂有限公司提供，批号：F20070418；Drugs: penicillin sodium, provided by Sinopharm Guorui Pharmaceutical Co., Ltd., batch number 20070518; gentian violet, provided by Sinopharm Chemical Reagent Co., Ltd., batch number: F20070418;
（三）试验方法(3) Test method
1．家兔皮肤大创口愈合试验：1. Rabbit skin large wound healing test:
家兔，去毛，在脊柱两侧分别切除1×2cm皮肤，用湿纱布浸润伤口1h后，给予相应护理品，7天后观察家兔伤口愈合率。Rabbits were depilated, and 1×2 cm of skin was excised on both sides of the spine, and the wound was infiltrated with wet gauze for 1 hour, then given corresponding nursing products, and the wound healing rate of the rabbit was observed after 7 days.
2．小鼠创口感染试验：2. Mouse wound infection test:
昆明种小鼠60只，随机分为：纯壳聚糖膜组；壳聚糖生物胶组；生理盐水组；生理盐水组。各组小鼠沿脊柱两侧脱毛，划#字口，金黄色葡萄球菌菌液涂抹伤口，于接菌后30min给予相应药物，每天重复一次，连续7d。分别记录各组死亡率。Sixty Kunming mice were randomly divided into: pure chitosan membrane group; chitosan biological glue group; normal saline group; normal saline group. The mice in each group were depilated along both sides of the spine, marked with #, and the Staphylococcus aureus bacterial solution was applied to the wound, and the corresponding drugs were given 30 minutes after inoculation, and repeated once a day for 7 consecutive days. The mortality rate of each group was recorded separately.
实验结果Experimental results
小鼠创口感染试验Mouse Wound Infection Test
结果可见，金黄色葡萄球菌可引起小属死亡，7天内的死亡率为50%，壳聚糖凝胶和壳聚糖+甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物组可明显保护伤口感染，7天内感染死亡率为20%；甘氨酸-L-半胱氨酸-高牛磺酸三肽无抗感染作用，7天内感染死亡率为50%。结果见下表2。The results showed that Staphylococcus aureus can cause the death of small genus, and the mortality rate within 7 days was 50%. Chitosan gel and chitosan+glycine-L-cysteine-homotaurine tripeptide composition group It can obviously protect wound infection, and the infection mortality within 7 days is 20%; glycine-L-cysteine-homotaurine tripeptide has no anti-infection effect, and the infection mortality within 7 days is 50%. The results are shown in Table 2 below.
表2.壳聚糖凝胶、三肽水溶液、壳聚糖与三肽组合物对小鼠金黄色葡萄球菌伤口Table 2. Chitosan gel, tripeptide aqueous solution, chitosan and tripeptide composition on mouse Staphylococcus aureus wound
感染死亡率的保护作用Protective effect on infection mortality
家兔皮肤大创口愈合试实验Healing Experiment of Rabbit Skin Large Wound
结果可见，家兔大伤口7天自然愈合率为50％；壳聚糖凝胶组7天愈合率为70％；甘氨酸-L-半胱氨酸-高牛磺酸三肽组7天愈合率为60％，壳聚糖+甘氨酸-L-半胱氨酸-高牛磺酸三肽组合物组7天愈合率为90％，结果见下表3。The results showed that the 7-day natural healing rate of large rabbit wounds was 50%; the 7-day healing rate of chitosan gel group was 70%; the 7-day healing rate of glycine-L-cysteine-homotaurine tripeptide group The 7-day healing rate of the chitosan+glycine-L-cysteine-homotaurine tripeptide composition group was 90%. The results are shown in Table 3 below.
表3.壳聚糖凝胶、三肽水溶液、壳聚糖与三肽组合物对家兔大伤口7天愈合作用观察Table 3. Observation of the healing effect of chitosan gel, tripeptide aqueous solution, chitosan and tripeptide composition on the large wound of rabbits in 7 days
应当理解的是，对本领域普通技术人员来说，可以根据上述说明加以改进或变换，而所有这些改进和变换都应属于本发明所附权利要求的保护范围。It should be understood that those skilled in the art can make improvements or changes based on the above description, and all these improvements and changes should belong to the protection scope of the appended claims of the present invention.
Claims (3)
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Non-Patent Citations (2)
| Title |
|---|
| G.Take et al.An investigation on skin wound healing in mice with a taurine-chitosan gel formulation.《Amino acids 》.2002,第22卷187-198. * |
| Tianhong Dai et al.Chitosan preparation for wounds and burns:antimicrobial and wound-healing effects.《NIH Public Access》.2011,第9卷(第7期),全文. * |
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