CN103461377B - Sterilization and withering agent and method for treating field diseased plants with banana vascular wilt by applying sterilization and withering agent - Google Patents

Abstract

The invention discloses a sterilization and withering agent and a method for treating field diseased plants with banana vascular wilt by applying the sterilization and withering agent. The bactericidal and withering agent provided by the invention can be composed of 0.045-4.5% by mass of prochloraz, 0.05-5% by mass of carbendazim, 0.041-4.1% by mass of glyphosate, 1-10% by mass of dimethyl sulfoxide, 1-10% by mass of glycol, 0.001-0.005% by mass of xanthan gum, 1-10% by mass of a dispersing agent and the balance of water. The invention provides powerful technical support for field prevention and control of banana vascular wilt, has quick effect and low cost, can effectively control the spread of diseases, reduce the morbidity and reduce the economic loss, has remarkable social and economic benefits and is very suitable for field popularization and application.

Description

A kind of bactericidal wilting agent and the method for using it to treat field diseased strains of banana wilt

technical field

The invention relates to a bactericidal and dead agent and a method for treating field diseased strains of banana fusarium wilt by using the same.

Background technique

Banana (Musa spp.) in a broad sense includes two categories: banana (Banana) and plantain (Plantain), which belong to the genus Musa (Musaece) and are the second largest fruit crop in the world, with output second only to citrus . Banana is a large perennial evergreen herb, monocotyledonous plant with huge biomass. The main varieties of bananas in my country are the Musa AAA group and the Dwarf Cavendish subgroup, namely Banana, with a plant height of 2-4 meters. Banana plants are composed of roots, bulbs, pseudostems, leaves, fruit shafts and ears. The base of the plant is called the corm (also known as the root bulb), and the main stalk formed by overlapping the leaf sheath is called the pseudostem (also known as the pseudostem). The corm, commonly known as the banana head, is the organ that takes root, leaves, and sucks buds, and is also the central part of supplying and storing nutrients.

Banana Fusarium Wilt, also known as Fusarium Fusarium Wilt, Panama Disease, and Yellow Leaf Disease, is a disease caused by Fusarium oxyporum f.sp.cubense that destroys plant vascular bundles and causes plant death. a devastating disease. At present, except for some island countries in the Mediterranean Sea, the Indian Ocean, and the South Pacific, almost all banana growing areas have been reported to suffer from the disease. The disease occurs in major banana planting areas such as Guangdong, Guangxi, Fujian, Hainan, Yunnan and Taiwan, and the incidence trend is obvious. The pathogenic bacteria of banana fusarium wilt can be transmitted through diseased plant residues, farming tools, seedlings with bacteria, soil and running water, etc., which makes the disease area continuously expand, spreads quickly, and is difficult to control. After the pathogen invades the host from the banana root system, it can colonize and spread in the vascular bundles, resulting in blockage of the vascular bundles, gradually yellowing the leaves of the plant, cracking the base of the pseudostem, and finally wilting and dying. At present, there is no ideal control agent for banana fusarium wilt, and banana orchards with higher incidence can only be abandoned or replanted with other crops. Therefore, when sporadic diseased strains appear in the field, measures should be taken in time to prevent the pathogen from spreading around the diseased plant residue.

At present, the methods for treating diseased plants of banana fusarium wilt in the field mainly include: ① cutting down the diseased plants and discarding them in the field to expose to the sun; ② sprinkling quicklime; ③ spraying herbicides for disinfection; The above methods are either simple to operate but unsatisfactory in disinfection effect, or have certain effects but complicated operation, which is not conducive to practical application in the field.

Contents of the invention

The object of the present invention is to provide a bactericidal and dead agent and a method for using it to treat field diseased strains of banana fusarium wilt.

The invention provides a fungicide and dry agent, including prochloraz, carbendazim and glyphosate, the mass percentage of the prochloraz is 0.045%-4.5% (such as 0.045%-0.45%, 0.45% -4.5%, 0.045%, 0.45% or 4.5%), the mass percentage of the carbendazim is 0.05%-5% (such as 0.05%-0.5%, 0.5%-5%, 0.05%, 0.5% or 5%), the mass percentage of glyphosate is 0.041%-4.1% (such as 0.041%-0.41%, 0.41%-4.1%, 0.041%, 0.41% or 4.1%).

The bactericidal desiccant can be made up of prochloraz, carbendazim, glyphosate, dimethyl sulfoxide, ethylene glycol, xanthan gum, dispersant and water, and the mass percentage of the prochloraz is 0.045%-4.5% (such as 0.045%-0.45%, 0.45%-4.5%, 0.045%, 0.45% or 4.5%), the mass percentage of carbendazim is 0.05%-5% (such as 0.05%- 0.5%, 0.5%-5%, 0.05%, 0.5% or 5%), the mass percentage of glyphosate is 0.041%-4.1% (such as 0.041%-0.41%, 0.41%-4.1%, 0.041 %, 0.41% or 4.1%), the mass percentage of dimethyl sulfoxide is 1%-10% (such as 1%-5%, 5%-10%, 1%, 5% or 10%) , the mass percentage of the ethylene glycol is 1%-10% (such as 1%-5%, 5%-10%, 1%, 5% or 10%), and the mass percentage of the xanthan gum The content is 0.001%-0.005% (such as 0.001%-0.003%, 0.003%-0.005%, 0.001%, 0.003% or 0.005%), and the mass percentage of the dispersant is 1-10% (such as 1%- 5%, 5%-10%, 1%, 5% or 10%), the balance is water.

The dispersant can specifically be sodium lignosulfonate.

The invention also protects a method for treating banana plants in the field with banana wilt, which includes the following steps: pouring the bactericidal and deadening agent into the banana plants with banana wilt in the field.

When the diameter of the banana plant where banana fusarium wilt occurs is less than or equal to 15cm, the perfusion site is one, that is, the pseudostem of the banana plant is 25-30cm away from the ground, and each perfusion site is perfused with 10-15mL of the bactericidal agent. Desiccant.

When the diameter of the banana plant with banana fusarium wilt occurred greater than 15cm, the perfusion site was two, i.e. the pseudostem of the banana plant was 25-30cm above the ground and the pseudostem was 50-60cm above the ground, each perfusion site Inject 20mL of the bactericidal and drying agent.

The perfusion can specifically be achieved by punching holes in the pseudostem of the banana plant. Specifically, the hole diameter of the perforated holes may be 2.5 cm. The method for punching holes can specifically be: make the punching tool form an angle of 45°-90° with the ground, insert the pseudostem for about 10-30 cm, and then pull it out. When pouring the pseudostem of the banana plant 25-30cm away from the ground, the method for punching holes can be: make the punching tool and the ground be at an angle of 60°-90°, insert the pseudostem about 25-30cm Then pull out. When pouring the pseudostem of the banana plant 50-60cm away from the ground, the method of punching holes can be: make the punching tool and the ground be at an angle of 45°-60°, insert the pseudostem about 10-15cm Then pull out.

The invention also protects the application of the bactericidal and dead agent in treating field banana plants with banana wilt.

The invention also protects the application of the bactericidal and dead agent in the prevention and treatment of banana wilt.

The present invention also protects a method for preventing and treating the banana wilt disease, which includes the step of treating the field banana plants with the banana wilt disease according to any of the above methods.

Any banana plant described above can be specifically a Brazilian banana plant.

In the bactericidal and desiccating agent of the present invention: Prochloraz and carbendazim, which have obvious inhibitory effects on the diseases of Deuteromycetes and Ascomycetes, are selected as effective bactericidal components. Certain systemic effect, and at the same time can avoid the occurrence of drug resistance; choose the systemic conduction type herbicide glyphosate as the drying component, which can dry up the diseased plants within 10-20 days, compared with 2-4 months The natural withering time is significantly shortened; dimethyl sulfoxide and ethylene glycol are selected as solubilizing ingredients to enhance permeability; xanthan gum has the functions of suspending, emulsifying, and stabilizing; dispersants can also enhance the mixing of various components Stability, which is conducive to the continuous and stable performance of the fungicide and dry agent.

Using the bactericidal and wilting agent provided by the invention to treat the field banana plants with banana wilt, the pathogens in the diseased plant tissues are killed while the diseased plants are dried up in a short period of time, so as to facilitate the transfer and destruction of the diseased plant residues the goal of. The method for treating field diseased strains of banana fusarium wilt provided by the present invention directly pours the bactericidal and deadening agent into the diseased plants, which is less affected by environmental factors and has quick results. It is convenient to transfer and destroy the remains of diseased plants, effectively avoiding the spread of germs in the field, with little environmental pollution and no adverse effects on other plants, saving time and labor.

The invention discloses a bactericidal and withering agent and a method for treating field diseased strains of banana fusarium wilt by using the bactericidal and withering agent. The invention solves the problems of huge biomass of diseased banana plants, difficulty in disinfection, long natural withering time, and easy spread of pathogens centered on the diseased plants in the field and causing large-scale disease, and provides a solution for the field prevention and control of banana fusarium wilt With strong technical support, quick results and low cost, it can effectively control the spread of diseases, reduce the incidence rate, and reduce economic losses. The social and economic benefits are remarkable, and it is very suitable for field application.

Detailed ways

The following examples facilitate a better understanding of the present invention, but do not limit the present invention. The experimental methods in the following examples are conventional methods unless otherwise specified. The test materials used in the following examples, unless otherwise specified, were purchased from conventional biochemical reagent stores. The banana plants in the embodiments are all Brazilian bananas (Musa AAACavendish subgroup 'Brazil'), also known as Brazilian bananas. Quantitative experiments in the following examples were all set up to repeat the experiments three times, and the results were averaged.

41% glyphosate water solution: Monsanto Company of the United States. 45% prochloraz water emulsion (also known as 450g/L prochloraz water emulsion): Bayer Crop Science Company.

Contrast medicament: mix 41% glyphosate aqueous solution 1ml, 45% prochloraz water emulsion 0.1ml and 29ml water.

Prochloraz technical (95%), light yellow solid powder, purchased from Guangzhou Yihetong Agrochemical Supply Chain Co., Ltd., CASNo. 67747-09-5, molecular weight 376.67, structural formula as follows:

Carbendazim technical (95%), white or light yellow-brown powder, purchased from Nanjing Aisen Fine Chemical Co., Ltd., CASNo. 10605-21-7, molecular weight 191.2, structural formula as follows:

Glyphosate technical (95%), non-volatile white solid, purchased from Shanghai Guiming Trading Co., Ltd., CAS No. 1071-83-6, molecular weight 169, structural formula as follows:

Xanthan collagen medicine (98%), off-white or light yellow powder, purchased from Shanghai Liqi Chemical Auxiliary Co., Ltd., CAS No. 11138-66-2, molecular weight about 2×10 ⁶ , structural formula as follows:

Sodium lignosulfonate technical product (96%), brown powder, purchased from Shanghai Puzhen Biotechnology Co., Ltd., CASNo. 8061-51-6, the structural formula is as follows:

The following punching tools are used in the examples: take iron water pipes or steel bars (about 0.025m in diameter and about 1.000-1.200m in length) commonly used in the field, and cut or grind one end obliquely into a pointed cone.

Embodiment 1, preparation and application of bactericidal dry agent

The preparation of KF1 bactericidal dry agent: Prochloraz original drug, carbendazim original drug, glyphosate original drug, dimethyl sulfoxide, ethylene glycol, xanthan collagen drug, sodium lignosulfonate original drug and Water is mixed and stirred evenly so that the mass percentage of prochloraz is 0.045%, the mass percentage of carbendazim is 0.05%, the mass percentage of glyphosate is 0.041%, and the mass percentage of dimethyl sulfoxide The percentage content is 1%, the mass percentage content of ethylene glycol is 1%, the mass percentage content of xanthan gum is 0.001%, the mass percentage content of sodium lignosulfonate is 1%, and the balance is water.

In late March 2013, in the Banana Fusarium Wilt Test Base in Danzhou City, Hainan Province, the following four groups of treatments were carried out:

Experimental group (randomly select 60 banana wilt diseased plants): For diseased plants with a diameter less than or equal to 15cm, punch a hole in the center of the corm at a place where the pseudostem is 25-30cm above the ground (the punching tool is 60°-90° away from the ground) ° angle, insert the pseudostem about 25-30cm and pull it out), perfuse each plant with 10-15mL KF1 fungicide; for diseased plants with a diameter greater than or equal to 15cm, shoot the pseudostem 25-30cm from the ground to the center of the bulb One hole (the angle between the punching tool and the ground is 60°-90°, insert the false stem about 25-30cm and then pull it out), and make another hole at a place 50-60cm away from the ground (the punching tool is 45° from the ground) °-60° angle, insert the pseudostem about 10-15cm and then pull it out), perfuse each plant with 40mL KF1 bactericidal and dead agent (that is, two holes are made for each diseased plant, and 20mL KF1 is perfused in each hole agent);

Control group-1 (randomly select 60 banana wilt diseased plants): For diseased plants with a diameter less than or equal to 20cm, inject 30mL of the control drug at a place 30cm above the ground with a medical syringe (see page 3 for ingredients, see below same), with an injection depth of about 10cm); for diseased plants with a diameter greater than 20cm, inject 60mL of the control drug at two points with a medical syringe at a place 30cm above the ground on the pseudostem (the injection depth is about 10cm, that is, inject 30mL at two symmetrical points of the diseased plant) contrast drug);

Control group-2 (60 banana fusarium wilt diseased plants were randomly selected): the control agent was used instead of the KF1 fungicidal and wilting agent, and the others were the same as the experimental group.

Control group-3 (60 plants with Fusarium wilt diseased plants randomly selected): No treatment was performed.

Start timing from perfusion or injection. On the 10th day, the part where the medicine was infused or injected was taken from the diseased strain, and the PDA plate culture method (combined with the observation of spore morphology) was used to detect the bacterial content of Fusarium oxysporum Cuban-specific type. Each plant of the control group-3 contained 1.151×10 ⁴ CFU of Fusarium oxysporum cuba-specific per gram of fresh weight on average. Compared with the control group-3, the amount of Fusarium oxysporum Cuban-specific type per gram fresh weight of the plants in the experimental group was reduced by 98.45%, and the amount of Fusarium oxysporum Cuban-specific type per gram fresh weight of the plants in the control group-2 The number was reduced by 85.38%, and the number of Fusarium oxysporum Cuban specialization per gram fresh weight of the control group-1 plant was reduced by 58.39%. On the 15th day, all the plants in the experimental group withered (that is, the wilting rate reached 100%), the wilting rate of the control group-1 was 90%, and the wilting rate of the control group-2 was 93%. The results show that the fungicide and dry agent provided by the invention and its supporting application method are significantly superior to the existing agents and application methods thereof, and are easy to operate in the field and easy to popularize in a large area.

Embodiment 2, preparation and application of bactericidal dry agent

The preparation of KF2 bactericidal dry agent: Prochloraz original drug, carbendazim original drug, glyphosate original drug, dimethyl sulfoxide, ethylene glycol, xanthan collagen drug, sodium lignosulfonate original drug and Water is mixed and stirred evenly so that the mass percentage of prochloraz is 0.45%, the mass percentage of carbendazim is 0.5%, the mass percentage of glyphosate is 0.41%, and the mass percentage of dimethyl sulfoxide The percentage content is 5%, the mass percentage content of ethylene glycol is 5%, the mass percentage content of xanthan gum is 0.003%, the mass percentage content of sodium lignosulfonate is 5%, and the balance is water.

In early April 2013, the following two groups of treatments were carried out in the Banana Test Base in Dongfang City, Hainan Province:

Experimental group (randomly select 20 banana wilt plants with a diameter of about 20cm): punch a hole in the center of the bulb at a place where the pseudostem is 25-30cm above the ground (the punching tool is at an angle of 60°-90° to the ground, insert the pseudostem The stem is pulled out after about 25-30cm), and another hole is drilled at a place 50-60cm away from the ground (the punching tool and the ground form an angle of 45°-60°, insert the fake stem about 10-15cm and pull it out), Inject 40mL of KF2 fungicide and dry agent into each plant (that is, two holes are made for each diseased plant, and 20mL of KF2 fungicide and dry agent is poured into each hole);

Control group (randomly select 20 banana wilt diseased plants with a diameter of about 20cm): no treatment.

Start timing from perfusion. On the 10th day, the part where the medicine was infused or injected was taken from the diseased strain, and the PDA plate culture method (combined with the observation of spore morphology) was used to detect the bacterial content of Fusarium oxysporum Cuban-specific type. Compared with the control group, the amount of Fusarium oxysporum cuba-specific per gram of fresh weight of the plants in the experimental group was reduced by 99.57%. On the 15th day, the drying rate of the experimental group reached 100%.

Embodiment 3, preparation and application of bactericidal dry agent

The preparation of KF3 bactericidal dry agent: Prochloraz original drug, carbendazim original drug, glyphosate original drug, dimethyl sulfoxide, ethylene glycol, xanthan collagen drug, sodium lignosulfonate original drug and Water is mixed and stirred evenly so that the mass percentage of prochloraz is 4.5%, the mass percentage of carbendazim is 5%, the mass percentage of glyphosate is 4.1%, the mass percentage of dimethyl sulfoxide The percentage content is 10%, the mass percentage content of ethylene glycol is 10%, the mass percentage content of xanthan gum is 0.005%, the mass percentage content of sodium lignosulfonate is 10%, and the balance is water.

In late May 2013, in the banana field near Xilian Farm in Danzhou City, Hainan Province, the following two groups of treatments were carried out:

Experimental group (randomly select 20 banana wilt plants with a diameter of about 20cm): punch a hole in the center of the bulb at a place where the pseudostem is 25-30cm above the ground (the punching tool is at an angle of 60°-90° to the ground, insert the pseudostem The stem is pulled out after about 25-30cm), and another hole is drilled at a place 50-60cm away from the ground (the punching tool and the ground form an angle of 45°-60°, insert the fake stem about 10-15cm and pull it out), Inject 40mL of KF3 bactericidal and dead agent into each plant (that is, two holes are made for each diseased plant, and 20mL of KF3 bactericidal and dead agent is poured into each hole);

Control group (randomly select 20 banana wilt diseased plants with a diameter of about 20cm): no treatment.

Start timing from perfusion. On the 10th day, the part where the medicine was infused or injected was taken from the diseased strain, and the PDA plate culture method (combined with the observation of spore morphology) was used to detect the bacterial content of Fusarium oxysporum Cuban-specific type. Compared with the control group, the number of Fusarium oxysporum cuba-specific per gram of fresh weight of the plants in the experimental group was reduced by 99.62%. On the 15th day, the drying rate of the experimental group reached 100%.

Claims (8)

1. a sterilization causes withered dose, by Prochloraz, carbendazim, glyphosate, dimethyl sulfoxide (DMSO), ethylene glycol, xanthans, sodium lignin sulfonate and water composition, the mass percentage of described Prochloraz is 0.045-4.5%, the mass percentage of described carbendazim is 0.05-5%, the mass percentage of described glyphosate is 0.041-4.1%, the mass percentage of described dimethyl sulfoxide (DMSO) is 1-10%, the mass percentage of described ethylene glycol is 1-10%, the mass percentage of described xanthans is 0.001-0.005%, the mass percentage of described sodium lignin sulfonate is 1-10%, surplus is water.

2. process a method for the land for growing field crops banana plant that banana blight occurs, comprise the steps: that sterilization according to claim 1 being caused withered dose of perfusion occurs in the banana plant of banana blight to large Tanaka.

3. method as claimed in claim 2, it is characterized in that: the diameter of the banana plant of described generation banana blight is less than or equal to 15cm, the position of described perfusion is one, i.e. the false stem of banana plant overhead 25-30cm place, and described in each perfusion position perfusion 10-15mL, sterilization causes withered dose.

4. method as claimed in claim 2, it is characterized in that: the diameter of the banana plant of described generation banana blight is greater than 15cm, the position of described perfusion is two, the i.e. false stem of banana plant overhead 25-30cm place and false stem overhead 50-60cm place, described in each perfusion position perfusion 20mL, sterilization causes withered dose.

5. as the method as described in arbitrary in claim 2 to 4, it is characterized in that: described perfusion is realized by punching on the false stem of banana plant that banana blight occurs.

6. sterilization according to claim 1 causes the withered dose of application occurred in process in the land for growing field crops banana plant of banana blight.

7. sterilization according to claim 1 causes the withered dose of application in control banana blight.

8. prevent and treat a method for banana blight, comprise the step being undertaken by described method arbitrary in claim 2 to 5 by the land for growing field crops banana plant that banana blight occurs processing.