CN103454254A - Detection unit of surface plasmon resonance (SPR) instrument - Google Patents
Detection unit of surface plasmon resonance (SPR) instrument Download PDFInfo
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- CN103454254A CN103454254A CN2013104064879A CN201310406487A CN103454254A CN 103454254 A CN103454254 A CN 103454254A CN 2013104064879 A CN2013104064879 A CN 2013104064879A CN 201310406487 A CN201310406487 A CN 201310406487A CN 103454254 A CN103454254 A CN 103454254A
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- 238000001514 detection method Methods 0.000 title claims abstract description 17
- 238000002198 surface plasmon resonance spectroscopy Methods 0.000 title abstract description 6
- 238000004458 analytical method Methods 0.000 claims description 5
- 230000003287 optical effect Effects 0.000 claims description 4
- 239000003086 colorant Substances 0.000 claims description 3
- 239000011159 matrix material Substances 0.000 claims description 3
- 238000005286 illumination Methods 0.000 claims 1
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- 239000000523 sample Substances 0.000 description 18
- 238000007405 data analysis Methods 0.000 description 5
- 238000005516 engineering process Methods 0.000 description 4
- 239000011248 coating agent Substances 0.000 description 2
- 238000000576 coating method Methods 0.000 description 2
- 238000013523 data management Methods 0.000 description 2
- 230000000694 effects Effects 0.000 description 2
- 238000000034 method Methods 0.000 description 2
- 239000012491 analyte Substances 0.000 description 1
- 230000005540 biological transmission Effects 0.000 description 1
- 230000008034 disappearance Effects 0.000 description 1
- 239000003446 ligand Substances 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004048 modification Effects 0.000 description 1
- 238000012986 modification Methods 0.000 description 1
- 230000001105 regulatory effect Effects 0.000 description 1
- 238000007493 shaping process Methods 0.000 description 1
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Abstract
The invention provides a detection unit of a surface plasmon resonance (SPR) instrument. The detection unit comprises a light source, a prism (1), an image sensor (2) and a data processing and analyzing system (3), and is characterized in that a multi-color narrow-band light-emitting diode (LED) light source (4) is adopted as the light source; the multi-color narrow-band LED light source (4) is arranged on one side of the prism (1); the image sensor (2) is arranged on the other side of the prism (1); the image sensor (2) is electrically connected with the data processing and analyzing system (3); the multi-color narrow-band LED light source (4) is fixedly arranged in the SPR instrument.
Description
Technical field
The present invention relates to a kind of SPR instrument probe unit, be specially the novel probe unit on a kind of SPR of being applied to instrument.
Background technology
Surface plasma resonance technology (Surface Plasmon Resonance technology, SPR) grows up the nineties in 20th century, a kind of new technology of ligand and analyte effect on application SPR principle detection of biological sensing chip.The SPR instrument that adopted on the market at present adopts the monochromatic LED light source more, because it can only radiate the narrow-band light source of single wavelength, therefore in detection process, need constantly the angle of incidence of light degree to be adjusted, strengthened detection time, reduced the detection efficiency of instrument, need to add regulating device simultaneously, increased to a certain extent cost of manufacture.
Summary of the invention
For the shortcoming that SPR instrument used need constantly be adjusted the angle of incidence of light degree on the market at present, the invention provides a kind of SPR instrument probe unit, comprise light source, prism 1, imageing sensor 2 and Data Management Analysis system 3, it is characterized in that, described light source adopts polychrome narrow-band LED light source 4, and described polychrome narrow-band LED light source 4 is positioned at described prism 1 one sides, described imageing sensor 2 is positioned at described prism 1 opposite side, and described imageing sensor 2 is electrically connected to described data analysis system 3, and wherein said polychrome narrow-band LED light source 4 is fixedly installed in described SPR instrument.
Preferably, described a kind of SPR instrument probe unit, the LED dot matrix that wherein said polychrome narrow-band LED light source 4 adopts with two or more different colours.
Preferably, described a kind of SPR instrument probe unit, it is 300nm-950nm that wherein said polychrome narrow-band LED light source 4 irradiates optical wavelength range.
Preferably, described a kind of SPR instrument probe unit, wherein said polychrome narrow-band LED light source 4 power are 1mw-500mw.
Preferably, described a kind of SPR instrument probe unit, wherein said prism 1 xsect is isosceles triangle.
Preferably, described a kind of SPR instrument probe unit, the irradiation light light path of wherein said polychrome narrow-band LED light source 4 is provided with collimated light lens and polaroid.
Preferably, described a kind of SPR instrument probe unit, wherein said imageing sensor 2 is positioned at described polychrome narrow-band LED light source 4 and irradiates on reflection of light light light path.
Preferably, described a kind of SPR instrument probe unit, 2 of wherein said prism 1 and described imageing sensors are provided with the lens of finding a view.
A kind of SPR instrument probe unit provided by the invention, by introducing polychrome narrow-band LED light source 4, and the Machine Design of employing fixed angle, a kind of simple designs without beam shaping optical element is provided, can have reached the effect of LASER Light Source, and the eliminate laser speckle impact, high-quality image, by software matching signal, can obtain the surface plasma resonance signal based on the wavelength-modulated method in addition, in the present invention simultaneously, polychrome narrow-band LED light source 4 adopts the fixed angle design.Save the device of modulation angle in the instrument, simplified equipment, reduced cost, improved stability and the reliability of instrument.
The accompanying drawing explanation
The structured flowchart that Fig. 1 is a kind of SPR instrument probe unit of proposing of the present invention.
Embodiment
Below in conjunction with accompanying drawing, the present invention is described in further detail, to make those skilled in the art, with reference to the instructions word, can implement according to this.
Please refer to Fig. 1, the invention provides a kind of SPR instrument probe unit, comprise light source, prism 1, imageing sensor 2 and Data Management Analysis system 3, it is characterized in that, described light source adopts polychrome narrow-band LED light source 4, and described polychrome narrow-band LED light source 4 is positioned at described prism 1 one sides, and described imageing sensor 2 is positioned at described prism 1 opposite side, and described imageing sensor 2 is electrically connected to described data analysis system 3, and wherein said polychrome narrow-band LED light source 4 is fixedly installed in described SPR instrument.
In the present invention,
The LED dot matrix that polychrome narrow-band LED light source 4 adopts with two or more different colours, it irradiates optical wavelength range is 300nm-950nm, power is 1mw-500mw.
Prism 1 xsect is isosceles triangle.
The irradiation light light path of polychrome narrow-band LED light source 4 is provided with collimated light lens and polaroid, and it sees through on the reflected light light path of prism and establishes imageing sensor 2, and establishes the lens of finding a view between prism 1 and imageing sensor 2.
A kind of SPR instrument probe unit provided by the invention, the detection chip that is of coupled connections above prism 1 top, this detection chip surface is coated with metallic reflective coating, and is provided with sample cell above detection chip.
Before the work of SPR instrument first by polychrome narrow-band LED light source 4, imageing sensor 2, data analysis system 3 energisings, whether detect each parts can work, then micro-example is splashed into to sample cell, the incident light that polychrome narrow-band LED light source 4 sends sees through prism 1 one sides, shine on the metallic reflective coating and determinand of prism 1 bottom, when total reflection phenomenon occurs in incident light on prism 1 and metallic film surface, can form the disappearance ripple, and there is certain plasma wave in determinand, when meeting, two ripples may resonate, and when resonance occurs, the reflected light light intensity can weaken significantly, the reflected light weakened sees through prism 1 opposite side and is received and be converted to digital data transmission to data analysis system 3 by imageing sensor 2, data analysis system 3 draws the response curve of reflected light light intensity.People, according to the resonant wavelength obtained the response curve from the reflected light light intensity and resonance angle, derive the refractive index of determinand, thus the composition of analysis place determinand.
A kind of SPR instrument probe unit provided by the invention, adopt polychrome narrow-band LED light source 4, the incident light of different wave length can be provided, compare current SPR instrument on the market, reduced the link of adjusting the incident light incident angle, polychrome narrow-band LED light source 4 directly can be fixed in instrument, improve the stability of instrument.
Although embodiment of the present invention are open as above, but it is not restricted to listed utilization in instructions and embodiment, it can be applied to various applicable the field of the invention fully, for those skilled in the art, can easily realize other modification, therefore do not deviating under the universal that claim and equivalency range limit, the present invention is not limited to specific details and illustrates here and the legend of describing.
Claims (8)
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
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| CN2013104064879A CN103454254A (en) | 2013-09-09 | 2013-09-09 | Detection unit of surface plasmon resonance (SPR) instrument |
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| CN2013104064879A CN103454254A (en) | 2013-09-09 | 2013-09-09 | Detection unit of surface plasmon resonance (SPR) instrument |
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Cited By (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103773852A (en) * | 2013-12-27 | 2014-05-07 | 中国科学院苏州生物医学工程技术研究所 | Kit used for detecting free miRNA (micro ribonucleic acid) in solution and operation method thereof |
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| WO1993014392A1 (en) * | 1992-01-11 | 1993-07-22 | Fisons Plc | Analytical device with polychromatic light source |
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2013
- 2013-09-09 CN CN2013104064879A patent/CN103454254A/en active Pending
Patent Citations (8)
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|---|---|---|---|---|
| WO1993014392A1 (en) * | 1992-01-11 | 1993-07-22 | Fisons Plc | Analytical device with polychromatic light source |
| CN1717581A (en) * | 2002-10-28 | 2006-01-04 | 华盛顿大学 | Wavelength Tunable Surface Plasmon Resonance Sensor |
| CN101319993A (en) * | 2007-05-03 | 2008-12-10 | 霍夫曼-拉罗奇有限公司 | oximeter |
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| CN101598667A (en) * | 2009-07-27 | 2009-12-09 | 国家海洋局第二海洋研究所 | Multi-excitation and multi-emission wavelength underwater organic fluorescence automatic detector in situ |
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| Title |
|---|
| 曾翰蓉: "基于SPR技术的检测装置设计与实验研究", 《中国优秀硕士学位论文全文数据库信息科技辑》, no. 09, 15 September 2008 (2008-09-15), pages 9 - 23 * |
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| Publication number | Priority date | Publication date | Assignee | Title |
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| CN103773852A (en) * | 2013-12-27 | 2014-05-07 | 中国科学院苏州生物医学工程技术研究所 | Kit used for detecting free miRNA (micro ribonucleic acid) in solution and operation method thereof |
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Application publication date: 20131218 |