CN103436445A - Cell culture bottle - Google Patents
Cell culture bottle Download PDFInfo
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- CN103436445A CN103436445A CN2013103796013A CN201310379601A CN103436445A CN 103436445 A CN103436445 A CN 103436445A CN 2013103796013 A CN2013103796013 A CN 2013103796013A CN 201310379601 A CN201310379601 A CN 201310379601A CN 103436445 A CN103436445 A CN 103436445A
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- China
- Prior art keywords
- face
- rear panel
- tissue culture
- culture flask
- front face
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- 238000004113 cell culture Methods 0.000 title abstract description 10
- 238000003466 welding Methods 0.000 claims description 6
- 238000007789 sealing Methods 0.000 claims description 3
- 238000000605 extraction Methods 0.000 abstract description 5
- 210000004027 cell Anatomy 0.000 description 10
- 238000012258 culturing Methods 0.000 description 8
- QVGXLLKOCUKJST-UHFFFAOYSA-N atomic oxygen Chemical compound [O] QVGXLLKOCUKJST-UHFFFAOYSA-N 0.000 description 2
- 210000004748 cultured cell Anatomy 0.000 description 2
- 229910052760 oxygen Inorganic materials 0.000 description 2
- 239000001301 oxygen Substances 0.000 description 2
- 238000000926 separation method Methods 0.000 description 2
- 230000009286 beneficial effect Effects 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 238000000151 deposition Methods 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 239000000284 extract Substances 0.000 description 1
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- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a cell culture bottle. A clamping edge of a front panel of the cell culture bottle can be clamped and positioned between a clamping groove and a flanged edge of a back panel of another cell culture bottle; and thus, a plurality of culture bottles can be stored and moved in a piling mode, thereby saving the space of an incubator and facilitating the management. The joint of the cell culture bottle comprises two triangular plates and a trapezoidal plate, and the included angle formed by the trapezoidal plate and the front panel is 120-135 degrees; the lower side panel, bottom panel and upper side panel tilt towards the inner side of the bottle body, the bottleneck tilts towards the back panel, and a certain inclination angle is set; and when the cell culture bottle is arranged flatly, a pipette or cell scraper can touch all positions of the culture bottom surface, thereby facilitating the collection of cells, and preventing the culture bottle from generating dead area where cells can not be easily extracted. The invention solves the following problems: the plurality of cell culture bottles can not be easily managed and stored, the cell culture bottle can not be subjected to extraction easily, and dead extraction areas can be easily generated.
Description
Technical field
The present invention relates to a kind of Tissue Culture Flask.
Background technology
Often use the container of vial as cell cultures in China's Med Biol research.When cultivating a large amount of cells, need a plurality of Tissue Culture Flasks, a plurality of Tissue Culture Flasks are manageability and depositing not.And present Tissue Culture Flask ubiquity is difficult to extraction, the easy problem of extracting dead angle that produces.
Summary of the invention
The present invention has overcome above-mentioned the deficiencies in the prior art, and a kind of Tissue Culture Flask is provided, and has solved a plurality of Tissue Culture Flasks and has been not easy management, deposits with the Tissue Culture Flask ubiquity and be difficult to extract, and easily produced the problem of extracting dead angle.
Technical scheme of the present invention is achieved in that
A kind of Tissue Culture Flask, described Tissue Culture Flask comprises bottle and bottle cap, and described bottle is tightly connected and is made by rear panel and body; Upper part of rear panel is trapezoidal, and lower part of rear panel is rectangle; The outside of rear panel and close top edge are provided with draw-in groove, and the outer margin contour outside rear panel is provided with some flange; Described body is integrated formed structure, includes front face, lower side panel face, latus superius face, junction surface, base plate face, bottleneck; Described front face is arranged on the right on of body and corresponding with rear panel; Upper part of front face is trapezoidal, and lower part of front face is rectangle, and the outer margin contour of front face is less than rear panel; Be provided with the some edges corresponding with draw-in groove on rear panel and flange along the outer margin contour in the front face outside, the edge on the front face of a Tissue Culture Flask can snap between the draw-in groove and flange on the rear panel that is positioned at another Tissue Culture Flask; Two lower side panel faces are respectively in lower part of the body left and right sides, and lower side panel is towards the inboard inclination of body; Described base plate face is positioned at the bottom of body and tilts towards the body inboard; In base plate face arranged outside, end rib is arranged; Two latus superius faces are respectively in upper part of the body left and right sides, and latus superius is towards the inboard inclination of body; The arranged outside of latus superius face has frosting; Described bottleneck is positioned at the body upper end, and bottleneck tilts towards rear panel; The downside of bottleneck is the arc surface structure; Described junction is between bottleneck and front face; Described junction surface includes two set square faces and a trapezoidal plate face, and the angle that the trapezoidal plate face becomes with front face is 120 ° to 135 °.
As preferably, described lower side panel face is provided with scale marks, can accurately put into appropriate substratum.
Keep flat when superimposed because of the closely separation difficulty of fitting for fear of a plurality of Tissue Culture Flasks, as preferably, be provided with the edge breach between described edge.
As preferably, described rear panel and body are by ultrasonic welding mode seal welding.
As preferably, the interior sidewall surface of described rear panel and body is processed through surface hydrophilicity, is conducive to the cell attachment well-grown.
In order to allow cultured cells obtain enough oxygen, as preferably, described bottle cap is provided with ventilating pit.
Enter culturing bottle for fear of extraneous foreign matter, as preferably, be provided with the sealing bottle holder in described bottle cap.
The invention has the beneficial effects as follows:
(1) keep flat when superimposed when a plurality of Tissue Culture Flasks, the edge on the front face of a Tissue Culture Flask can snap between the draw-in groove and flange on the rear panel that is positioned at another Tissue Culture Flask; A plurality of like this culturing bottles can stack to be deposited and moves, and saves the incubator space, convenient management.
(2) the latus superius face of described culturing bottle is provided with frosting, is convenient to the marking pen mark.
(3) be provided with bottleneck in the upper end of body, bottleneck tilts towards rear panel, and the downside of bottleneck is the arc surface structure.When cell cultures keeps flat, position of bottleneck is higher like this, while using more substratum, can not overflow, and the convenient cell that extracts from culturing bottle.
(4) described junction surface includes two set squares and a trapezoidal plate, and the angle that trapezoidal plate becomes with front face is 120 ° to 135 °; Lower side panel face, base plate face, latus superius tilt towards rear panel towards the inboard inclination of body, bottleneck, and be provided with certain angle of inclination, like this, when Tissue Culture Flask keeps flat, transfer pipet or cell scraper can touch all positions of cultivating bottom surface, facilitate harvested cell, avoid culturing bottle to produce the extraction dead angle that is difficult for extracting cell.
The accompanying drawing explanation
Fig. 1 is structural representation of the present invention;
The side-view that Fig. 2 is Fig. 1;
The rear view that Fig. 3 is Fig. 1;
The A-A that Fig. 4 is Fig. 1 is to schematic cross-section;
The upward view that Fig. 5 is Fig. 1;
The structural representation that Fig. 6 is bottle cap in embodiment 1.
1, rear panel; 12, draw-in groove; 13, flange; 2, body; 21, front face; 211, edge; 212, edge breach; 22, lower side panel face; 23, latus superius face; 231, frosting; 24, bottleneck; 241, downside; 25, junction surface; 251, set square face; 252, trapezoidal plate face; 26, base plate face; 261, end rib; 3, bottle cap.
Embodiment
The specific embodiment of the present invention is as follows:
Embodiment 1: a kind of Tissue Culture Flask, and as shown in Fig. 1,2,3,5, described Tissue Culture Flask comprises bottle and bottle cap 3, and described bottle is formed by ultrasonic welding mode seal welding by rear panel 1 and body 2.The interior sidewall surface of described rear panel 1 and body 2 is processed through surface hydrophilicity, is conducive to the cell attachment well-grown.Upper part of rear panel 1 is trapezoidal, and lower part of rear panel 1 is rectangle; The outside of rear panel 1 and be provided with draw-in groove 12 near top edge, be provided with some flange 13 along the outer margin contour in rear panel 1 outside; Described body 2 is integrated formed structures, includes front face 21, lower side panel face 22, latus superius face 23, junction surface 25, base plate face 26, bottleneck 24; Described front face 21 is arranged on the right on of body 2 and corresponding with rear panel 1; Upper part of front face 21 is trapezoidal, and lower part of front face 21 is rectangle, and the outer margin contour of front face 21 is less than rear panel 1; The edge 211 be provided with along the outer margin contour in front face 21 outsides on the front face 21 of 211, one Tissue Culture Flasks of some edges corresponding with draw-in groove 12 on rear panel 1 and flange 13 can snap between the draw-in groove 12 and flange 13 on the rear panel 1 that is positioned at another Tissue Culture Flask; When a plurality of Tissue Culture Flasks keep flat when superimposed, the edge 211 on the front face 21 of a Tissue Culture Flask can snap between the draw-in groove 12 and flange 13 on the rear panel 1 that is positioned at another Tissue Culture Flask; A plurality of like this culturing bottles can stack to be deposited and moves, and saves the incubator space, convenient management.Be provided with edge breach 212 between described edge 211, avoid a plurality of Tissue Culture Flasks to keep flat when superimposed because of the closely separation difficulty of fitting.
As shown in Fig. 1,2,5, two lower side panel faces 22 are respectively in lower part of body 2 left and right sides, and lower side panel face 22 is to the inboard inclination of body 2; Described base plate face 26 is positioned at the bottom of body 2 and tilts towards body 2 inboards; In base plate face 26 arranged outside, end rib 261 is arranged; Two latus superius faces 23 are respectively in upper part of body 2 left and right sides, and latus superius face 23 is to the inboard inclination of body 2; The arranged outside of latus superius face 23 has frosting 231, is convenient to the marking pen mark; Described bottleneck 24 is positioned at body 2 upper ends, and bottleneck 24 tilts towards rear panel 1; The downside 241 of bottleneck 24 is the arc surface structure; Described junction surface 25 is between bottleneck 24 and front face 21; Described junction surface 25 includes two set square faces 251 and a trapezoidal plate face 252, and the angle of trapezoidal plate face 252 and 21 one-tenth of front faces is for being 120 ° to 135 °.And lower side panel face 22, base plate face 26, latus superius face 23 towards body 2 inboardly tilt, bottleneck 24 tilts towards rear panel 1, and be provided with certain angle of inclination, like this when Tissue Culture Flask keeps flat, transfer pipet or cell scraper can touch all positions of cultivating bottom surface, avoid culturing bottle to produce the extraction dead angle that is difficult for extracting cell, facilitate harvested cell and, position of bottleneck is higher, while using more substratum, can not overflow; Described lower side panel face 22 is provided with scale marks, can accurately put into appropriate substratum.
As shown in Figure 6, described bottle cap 3 is provided with ventilating pit, can allow cultured cells obtain enough oxygen.
Embodiment 2: a kind of Tissue Culture Flask, as shown in Figure 1, 2, 3, embodiment 2 and unique not being both of embodiment 1: be provided with the sealing bottle holder in bottle cap 3, avoid extraneous foreign matter to enter culturing bottle; Rest part is identical.
Claims (7)
1. a Tissue Culture Flask, is characterized in that, described Tissue Culture Flask comprises bottle and bottle cap (3), and described bottle is tightly connected and is made by rear panel (1) and body (2); Upper part of rear panel (1) is trapezoidal, and lower part of rear panel (1) is rectangle; The outside of rear panel (1) and close top edge are provided with draw-in groove (12), and the outer margin contour outside rear panel (1) is provided with some flange (13); Described body (2) is integrated formed structure, includes front face (21), lower side panel face (22), latus superius face (23), junction surface (25), base plate face (26), bottleneck (24); Described front face (21) is arranged on the right on of body (2) and corresponding with rear panel (1); Upper part of front face (21) is trapezoidal, and lower part of front face (21) is rectangle, and the outer margin contour of front face (21) is less than rear panel (1); Be provided with the some edges (211) corresponding with draw-in groove (12) on rear panel (1) and flange (13) along the outer margin contour in front face (21) outside, the edge (211) on the front face of a Tissue Culture Flask (21) can snap between the draw-in groove (12) and flange (13) on the rear panel (1) that is positioned at another Tissue Culture Flask; Two lower side panel faces (22) are respectively in lower part of body (2) left and right sides, and lower side panel face (22) is to the inboard inclination of body (2); Described base plate face (26) is positioned at the bottom of body (2) and tilts towards body (2) inboard; In base plate face (26) arranged outside, end rib (261) is arranged; Two latus superius faces (23) are respectively in upper part of body (2) left and right sides, and latus superius face (23) is to the inboard inclination of body (2); The arranged outside of latus superius face (23) has frosting (231); Described bottleneck (24) is positioned at body (2) upper end, and bottleneck (24) tilts towards rear panel (1); The downside (241) of bottleneck (24) is the arc surface structure; Described junction surface (25) is positioned between bottleneck (24) and front face (21); Described junction surface (25) includes two set square faces (251) and a trapezoidal plate face (252), and the angle that trapezoidal plate face (252) becomes with front face (21) is 120 ° to 135 °.
2. a kind of Tissue Culture Flask according to claim 1, is characterized in that, described lower side panel face (22) is provided with scale marks.
3. a kind of Tissue Culture Flask according to claim 1, is characterized in that, is provided with edge breach (212) between described edge (211).
4. a kind of Tissue Culture Flask according to claim 1, is characterized in that, described rear panel (1) and body (2) are by ultrasonic welding mode seal welding.
5. a kind of Tissue Culture Flask according to claim 1, is characterized in that, the interior sidewall surface of described rear panel (1) and body (2) is processed through surface hydrophilicity.
6. a kind of Tissue Culture Flask according to claim 1, is characterized in that, described bottle cap (3) is provided with ventilating pit.
7. a kind of Tissue Culture Flask according to claim 1, is characterized in that, described bottle cap is provided with the sealing bottle holder in (3).
Priority Applications (1)
Application Number | Priority Date | Filing Date | Title |
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CN2013103796013A CN103436445A (en) | 2013-08-27 | 2013-08-27 | Cell culture bottle |
Applications Claiming Priority (1)
Application Number | Priority Date | Filing Date | Title |
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CN2013103796013A CN103436445A (en) | 2013-08-27 | 2013-08-27 | Cell culture bottle |
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CN103436445A true CN103436445A (en) | 2013-12-11 |
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CN2013103796013A Pending CN103436445A (en) | 2013-08-27 | 2013-08-27 | Cell culture bottle |
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Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109401960A (en) * | 2017-08-16 | 2019-03-01 | 苏州米迪生物技术有限公司 | The high efficiency cell culture bottle of built-in disturbing flow device |
Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US4770854A (en) * | 1986-02-03 | 1988-09-13 | Costar Corporation | Laboratory flask |
US5139952A (en) * | 1983-09-16 | 1992-08-18 | Terumo Corporation | Tissue culture flask |
CN201003056Y (en) * | 2007-02-12 | 2008-01-09 | 袁建华 | Cell culture bottle |
CN203419928U (en) * | 2013-08-27 | 2014-02-05 | 浙江硕华医用塑料有限公司 | Cell culture bottle |
-
2013
- 2013-08-27 CN CN2013103796013A patent/CN103436445A/en active Pending
Patent Citations (4)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
US5139952A (en) * | 1983-09-16 | 1992-08-18 | Terumo Corporation | Tissue culture flask |
US4770854A (en) * | 1986-02-03 | 1988-09-13 | Costar Corporation | Laboratory flask |
CN201003056Y (en) * | 2007-02-12 | 2008-01-09 | 袁建华 | Cell culture bottle |
CN203419928U (en) * | 2013-08-27 | 2014-02-05 | 浙江硕华医用塑料有限公司 | Cell culture bottle |
Cited By (1)
Publication number | Priority date | Publication date | Assignee | Title |
---|---|---|---|---|
CN109401960A (en) * | 2017-08-16 | 2019-03-01 | 苏州米迪生物技术有限公司 | The high efficiency cell culture bottle of built-in disturbing flow device |
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Application publication date: 20131211 |