CN103430846B - Culture medium composition for strawberry tissue culture - Google Patents
Culture medium composition for strawberry tissue culture Download PDFInfo
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- CN103430846B CN103430846B CN201310351128.8A CN201310351128A CN103430846B CN 103430846 B CN103430846 B CN 103430846B CN 201310351128 A CN201310351128 A CN 201310351128A CN 103430846 B CN103430846 B CN 103430846B
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- 239000000203 mixture Substances 0.000 title claims abstract description 30
- 235000016623 Fragaria vesca Nutrition 0.000 title claims abstract description 29
- 235000011363 Fragaria x ananassa Nutrition 0.000 title claims abstract description 29
- 239000001963 growth medium Substances 0.000 title claims abstract description 28
- 240000009088 Fragaria x ananassa Species 0.000 title 1
- 241000220223 Fragaria Species 0.000 claims abstract description 29
- 230000001939 inductive effect Effects 0.000 claims abstract description 6
- 239000002609 medium Substances 0.000 claims description 17
- 229920001817 Agar Polymers 0.000 claims description 14
- 229920002148 Gellan gum Polymers 0.000 claims description 14
- 239000000451 gelidium spp. gum Substances 0.000 claims description 14
- 239000000216 gellan gum Substances 0.000 claims description 14
- 235000010492 gellan gum Nutrition 0.000 claims description 14
- 238000009395 breeding Methods 0.000 claims description 12
- 230000001488 breeding effect Effects 0.000 claims description 12
- 239000005556 hormone Substances 0.000 claims description 12
- 229940088597 hormone Drugs 0.000 claims description 12
- 239000007640 basal medium Substances 0.000 claims description 10
- 239000000499 gel Substances 0.000 claims description 10
- NLKNQRATVPKPDG-UHFFFAOYSA-M potassium iodide Chemical compound [K+].[I-] NLKNQRATVPKPDG-UHFFFAOYSA-M 0.000 claims description 9
- 239000006160 differential media Substances 0.000 claims description 7
- 230000006698 induction Effects 0.000 claims description 7
- DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 claims description 6
- CSNNHWWHGAXBCP-UHFFFAOYSA-L Magnesium sulfate Chemical compound [Mg+2].[O-][S+2]([O-])([O-])[O-] CSNNHWWHGAXBCP-UHFFFAOYSA-L 0.000 claims description 6
- PVNIIMVLHYAWGP-UHFFFAOYSA-N Niacin Chemical compound OC(=O)C1=CC=CN=C1 PVNIIMVLHYAWGP-UHFFFAOYSA-N 0.000 claims description 6
- RBTARNINKXHZNM-UHFFFAOYSA-K iron trichloride Chemical compound Cl[Fe](Cl)Cl RBTARNINKXHZNM-UHFFFAOYSA-K 0.000 claims description 6
- 239000005416 organic matter Substances 0.000 claims description 6
- FGIUAXJPYTZDNR-UHFFFAOYSA-N potassium nitrate Chemical compound [K+].[O-][N+]([O-])=O FGIUAXJPYTZDNR-UHFFFAOYSA-N 0.000 claims description 6
- 239000011573 trace mineral Substances 0.000 claims description 6
- 235000013619 trace mineral Nutrition 0.000 claims description 6
- PAWQVTBBRAZDMG-UHFFFAOYSA-N 2-(3-bromo-2-fluorophenyl)acetic acid Chemical compound OC(=O)CC1=CC=CC(Br)=C1F PAWQVTBBRAZDMG-UHFFFAOYSA-N 0.000 claims description 3
- IBWNNZYMBIZELM-UHFFFAOYSA-N 4,5-bis(hydroxymethyl)-2-methylpyridin-3-ol;pyridine-3-carboxylic acid Chemical compound OC(=O)C1=CC=CN=C1.CC1=NC=C(CO)C(CO)=C1O IBWNNZYMBIZELM-UHFFFAOYSA-N 0.000 claims description 3
- 239000003109 Disodium ethylene diamine tetraacetate Substances 0.000 claims description 3
- ZGTMUACCHSMWAC-UHFFFAOYSA-L EDTA disodium salt (anhydrous) Chemical compound [Na+].[Na+].OC(=O)CN(CC([O-])=O)CCN(CC(O)=O)CC([O-])=O ZGTMUACCHSMWAC-UHFFFAOYSA-L 0.000 claims description 3
- 239000004471 Glycine Substances 0.000 claims description 3
- 229930006000 Sucrose Natural products 0.000 claims description 3
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 3
- KGBXLFKZBHKPEV-UHFFFAOYSA-N boric acid Chemical compound OB(O)O KGBXLFKZBHKPEV-UHFFFAOYSA-N 0.000 claims description 3
- 239000004327 boric acid Substances 0.000 claims description 3
- LLSDKQJKOVVTOJ-UHFFFAOYSA-L calcium chloride dihydrate Chemical compound O.O.[Cl-].[Cl-].[Ca+2] LLSDKQJKOVVTOJ-UHFFFAOYSA-L 0.000 claims description 3
- 229940052299 calcium chloride dihydrate Drugs 0.000 claims description 3
- 229910000428 cobalt oxide Inorganic materials 0.000 claims description 3
- IVMYJDGYRUAWML-UHFFFAOYSA-N cobalt(ii) oxide Chemical compound [Co]=O IVMYJDGYRUAWML-UHFFFAOYSA-N 0.000 claims description 3
- ARUVKPQLZAKDPS-UHFFFAOYSA-L copper(II) sulfate Chemical compound [Cu+2].[O-][S+2]([O-])([O-])[O-] ARUVKPQLZAKDPS-UHFFFAOYSA-L 0.000 claims description 3
- 235000019301 disodium ethylene diamine tetraacetate Nutrition 0.000 claims description 3
- 229940064880 inositol 100 mg Drugs 0.000 claims description 3
- SURQXAFEQWPFPV-UHFFFAOYSA-L iron(2+) sulfate heptahydrate Chemical group O.O.O.O.O.O.O.[Fe+2].[O-]S([O-])(=O)=O SURQXAFEQWPFPV-UHFFFAOYSA-L 0.000 claims description 3
- 229910052943 magnesium sulfate Inorganic materials 0.000 claims description 3
- 235000019341 magnesium sulphate Nutrition 0.000 claims description 3
- 239000011702 manganese sulphate Substances 0.000 claims description 3
- 235000007079 manganese sulphate Nutrition 0.000 claims description 3
- BZDIAFGKSAYYFC-UHFFFAOYSA-N manganese;hydrate Chemical compound O.[Mn] BZDIAFGKSAYYFC-UHFFFAOYSA-N 0.000 claims description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 claims description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 claims description 3
- 239000006870 ms-medium Substances 0.000 claims description 3
- 229960003512 nicotinic acid Drugs 0.000 claims description 3
- 235000001968 nicotinic acid Nutrition 0.000 claims description 3
- 239000011664 nicotinic acid Substances 0.000 claims description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 claims description 3
- 239000004323 potassium nitrate Substances 0.000 claims description 3
- 235000010333 potassium nitrate Nutrition 0.000 claims description 3
- 239000011684 sodium molybdate Substances 0.000 claims description 3
- 235000015393 sodium molybdate Nutrition 0.000 claims description 3
- TVXXNOYZHKPKGW-UHFFFAOYSA-N sodium molybdate (anhydrous) Chemical compound [Na+].[Na+].[O-][Mo]([O-])(=O)=O TVXXNOYZHKPKGW-UHFFFAOYSA-N 0.000 claims description 3
- 239000002904 solvent Substances 0.000 claims description 3
- 239000005720 sucrose Substances 0.000 claims description 3
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 3
- NWONKYPBYAMBJT-UHFFFAOYSA-L zinc sulfate Chemical compound [Zn+2].[O-]S([O-])(=O)=O NWONKYPBYAMBJT-UHFFFAOYSA-L 0.000 claims description 3
- 239000011686 zinc sulphate Substances 0.000 claims description 3
- 235000009529 zinc sulphate Nutrition 0.000 claims description 3
- 238000000034 method Methods 0.000 abstract description 9
- 239000011159 matrix material Substances 0.000 abstract description 6
- 235000015097 nutrients Nutrition 0.000 abstract description 5
- 230000004083 survival effect Effects 0.000 abstract description 4
- 201000002451 Overnutrition Diseases 0.000 abstract description 3
- 230000007547 defect Effects 0.000 abstract description 3
- 235000020823 overnutrition Nutrition 0.000 abstract description 3
- 208000002720 Malnutrition Diseases 0.000 abstract 1
- 235000000112 undernutrition Nutrition 0.000 abstract 1
- 235000013399 edible fruits Nutrition 0.000 description 3
- 238000005286 illumination Methods 0.000 description 3
- 208000015380 nutritional deficiency disease Diseases 0.000 description 3
- 239000002689 soil Substances 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 2
- 235000016709 nutrition Nutrition 0.000 description 2
- 230000035764 nutrition Effects 0.000 description 2
- 241000196324 Embryophyta Species 0.000 description 1
- 208000018522 Gastrointestinal disease Diseases 0.000 description 1
- 208000007502 anemia Diseases 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 235000013361 beverage Nutrition 0.000 description 1
- 235000012970 cakes Nutrition 0.000 description 1
- 239000002131 composite material Substances 0.000 description 1
- 238000012364 cultivation method Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 208000010643 digestive system disease Diseases 0.000 description 1
- 201000010099 disease Diseases 0.000 description 1
- 208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 235000013305 food Nutrition 0.000 description 1
- 235000015203 fruit juice Nutrition 0.000 description 1
- 208000018685 gastrointestinal system disease Diseases 0.000 description 1
- 238000003898 horticulture Methods 0.000 description 1
- 235000015094 jam Nutrition 0.000 description 1
- 239000012092 media component Substances 0.000 description 1
- 238000009335 monocropping Methods 0.000 description 1
- 239000000758 substrate Substances 0.000 description 1
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- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
The invention discloses culture medium composition for strawberry tissue culture. During the whole strawberry growth process, matrix for basic growth and matrix for inducing growth and propagation growth are separately prepared, and with the matching of components in different proportions for the culture media, nutrient supply of the culture medium is optimized, the nutrient balance during the strawberry growth process is ensured, defects of overnutrition or undernutrition, which are easy to appear in each phase of the traditional culture medium, are effectively overcome, the rooting percentage of the strawberry and the stem survival rate are increased, and the seedling quality is improved.
Description
Technical field
The present invention relates to the formula of crops medium, particularly relate to a kind of culture media composition of Strawberry tissue culture.
Background technology
Strawberry is rose family Fragaria perennial root perennial evergreen herbaceous plant, is worldwide fruit, also has certain medical value, have certain curative effect to gastrointestinal disease, anaemia.Strawberry is ripe fruit the earliest in a bit, the end of spring and the beginning of summer can carry out gathering putting on market, its industrial chain is longer, the various food such as fruit juice, jam, beverage, cake and preserved fruit can be processed into, but strawberry production for a long time, all rely on traditional soil cultivation division propagation method to produce seedling, the problems such as traditional soil cultivation method labour intensity is large, fruiting period is short, continuous cropping obstacle become increasingly conspicuous, and limiting strawberry further develops; Along with developing rapidly of facilities horticulture, cultivation technique without soil is just in large area, apply on a large scale, some safety, high-quality, convenience, good composite cultivation substrate are constantly developed, synthesize and use, but existing medium in the overall process that strawberry grows not by matrix required during based growth and induced growth, expand numerous growth time required matrix separate and prepare, thus result in the superfluous and later stage induced growth of based growth process Middle nutrition and may occur malnourished phenomenon.
Summary of the invention
Goal of the invention: in order to overcome the deficiencies in the prior art, the present invention proposes a kind of can active balance Strawberry tissue culture different growing stage nutrient, improve the culture media composition of a kind of Strawberry tissue culture of rooting rate and stem apex survival rate.
Technical scheme: in order to solve the problems of the technologies described above, the technical solution adopted in the present invention is: a kind of culture media composition of Strawberry tissue culture: comprise basal medium, inducing culture and expansion breeding culture medium: each component and contained weight as follows:
(1) basal medium: 1/4 improvement MS, gel 3-5g/L, pH is adjusted to 5-6;
(2) induced bud differential medium: improvement MS, gel 6-8g/L, hormone, pH is adjusted to 5.5-6;
(3) root induction medium: 1/2 improvement MS, gel 4-6g/L, hormone, pH is adjusted to 5.5-6;
(4) expand breeding culture medium: improvement MS, gel 6-8g/L, hormone, pH is adjusted to 5.5-6;
The solvent of the MS medium of described improvement is water, and composition comprises macroelement, trace element, molysite and organic matter, concrete component and corresponding concentration as follows:
Wherein macroelement comprises potassium nitrate 1700mg/L, ammonium nitrate 1600mg/L, epsom salt 300mg/L, anhydrous potassium dihydrogenphosphate 150mg/L, calcium chloride dihydrate 450mg/L and disodium ethylene diamine tetraacetate 37mg/L;
Its medium trace element comprises four water manganese sulphate 23.1mg/L, zinc sulphate 9mg/L, boric acid 5.9mg/L, potassium iodide 0.7mg/L, sodium molybdate 0.2mg/L, copper sulphate 0.2mg/L and cobalt oxide 0.03mg/L;
Wherein molysite is ferrous sulfate heptahydrate 26.5mg/L;
Wherein organic matter comprises inositol 100mg/L, glycine 3mg/L, nicotinic acid 0.5mg/L, sucrose 35g/L and nicotinic acid pyridoxine 0.5mg/L.
More preferred, described gel is the mixture of agar and gellan gum, and mass ratio is 3:1.
Further, in described induced bud differential medium, hormone comprises 2,4-D0.3mg/L and 6BA0.3mg/L.
Further, in described root induction medium, hormone comprises NAA0.2mg/L and 6BA0.2mg/L.
Further, in described expansion breeding culture medium, hormone is 2,4-D0.2mg/L, NAA0.1mg/L and 6BA0.2mg/L.
Beneficial effect: the culture media composition of a kind of Strawberry tissue culture provided by the invention; in the overall process of strawberry growth by matrix required during based growth and induced growth, expand numerous growth time required matrix separate and prepare; be equipped with different media components proportionings; optimize medium nutrition supply; ensure that the nutrient in strawberry process of growth is balanced; easily there is overnutrition or malnourished defect in each stage effectively overcoming conventional medium; improve strawberry rooting rate and stem apex survival rate, improve Quality of Seedlings.
Embodiment
Below in conjunction with specific embodiment, the present invention is described in further detail:
Embodiment 1:
A kind of culture media composition of Strawberry tissue culture: comprise basal medium, inducing culture and expansion breeding culture medium: each component and contained weight as follows:
(1) basal medium: mixture (mass ratio is 3:1) 3g/L, the pH of 1/4 improvement MS, agar and gellan gum are adjusted to 5.0;
(2) induced bud differential medium: mixture (mass ratio the is 3:1) 6g/L of improvement MS, agar and gellan gum, 2,4-D0.3mg/L, 6BA0.3mg/L, pH be adjusted to 5.5;
(3) root induction medium: mixture (mass ratio is 3:1) 4g/L, NAA0.2mg/L, 6BA0.2mg/L of 1/2 improvement MS, agar and gellan gum, pH is adjusted to 5.5;
(4) expand breeding culture medium: improvement MS, agar and gellan gum mixture (mass ratio is 3:1) 6g/L, 2,4-D0.2mg/L, NAA0.1mg/L, 6BA0.2mg/L, pH is adjusted to 5.5.
Embodiment 2:
A kind of culture media composition of Strawberry tissue culture: comprise basal medium, inducing culture and expansion breeding culture medium: each component and contained weight as follows:
(1) basal medium: mixture (mass ratio is 3:1) 5g/L, the pH of 1/4 improvement MS, agar and gellan gum are adjusted to 6.0;
(2) induced bud differential medium: mixture (mass ratio the is 3:1) 8g/L of improvement MS, agar and gellan gum, 2,4-D0.3mg/L, 6BA0.3mg/L, pH be adjusted to 6.0;
(3) root induction medium: mixture (mass ratio is 3:1) 6g/L, NAA0.2mg/L, 6BA0.2mg/L of 1/2 improvement MS, agar and gellan gum, pH is adjusted to 6.0;
(4) expand breeding culture medium: improvement MS, agar and gellan gum mixture (mass ratio is 3:1) 8g/L, 6g/L, 2,4-D0.2mg/L, NAA0.1mg/L, 6BA0.2mg/L, pH is adjusted to 6.0.
Embodiment 3:
A kind of culture media composition of Strawberry tissue culture: comprise basal medium, inducing culture and expansion breeding culture medium: each component and contained weight as follows:
(1) basal medium: mixture (mass ratio is 3:1) 4g/L, the pH of 1/4 improvement MS, agar and gellan gum are adjusted to 5.5;
(2) induced bud differential medium: mixture (mass ratio the is 3:1) 7g/L of improvement MS, agar and gellan gum, 2,4-D0.3mg/L, 6BA0.3mg/L, pH be adjusted to 5.7;
(3) root induction medium: mixture (mass ratio is 3:1) 5g/L, NAA0.2mg/L, 6BA0.2mg/L of 1/2 improvement MS, agar and gellan gum, pH is adjusted to 5.7;
(4) expand breeding culture medium: improvement MS, agar and gellan gum mixture (mass ratio is 3:1) 7g/L, 6g/L, 2,4-D0.2mg/L, NAA0.1mg/L, 6BA0.2mg/L, pH is adjusted to 5.7.
In above-described embodiment 1-3, the solvent of the MS medium of described improvement is water, and composition comprises macroelement, trace element, molysite and organic matter, concrete component and corresponding concentration as follows:
Wherein macroelement comprises potassium nitrate 1700mg/L, ammonium nitrate 1600mg/L, epsom salt 300mg/L, anhydrous potassium dihydrogenphosphate 150mg/L, calcium chloride dihydrate 450mg/L and disodium ethylene diamine tetraacetate 37mg/L;
Its medium trace element comprises four water manganese sulphate 23.1mg/L, zinc sulphate 9mg/L, boric acid 5.9mg/L, potassium iodide 0.7mg/L, sodium molybdate 0.2mg/L, copper sulphate 0.2mg/L and cobalt oxide 0.03mg/L;
Wherein molysite is ferrous sulfate heptahydrate 26.5mg/L;
Wherein organic matter comprises inositol 100mg/L, glycine 3mg/L, nicotinic acid 0.5mg/L, sucrose 35g/L and nicotinic acid pyridoxine 0.5mg/L.
The 5-6 month, select the afternoon of fine day, the stem apex 180 that the stolon that healthy and strong, disease-free strawberry have just been extracted out is chosen 0.3-0.5mm is cultivated respectively as strawberry explant on the medium described in 3 embodiments, condition of culture for carry out illumination cultivation at temperature 25 ± 2 DEG C, 16 h light, intensity of illumination 10 ~ 30 μm
-2.s
-1; Be 20 ± 2 DEG C in temperature and carry out light culture, light culture 6 hours, above-mentioned illumination and light culture hocket.
The culture media composition of a kind of Strawberry tissue culture of the present invention is used to cultivate; ensure that the nutrient in strawberry process of growth is balanced; easily there is overnutrition or malnourished defect in each stage effectively overcoming conventional medium; strawberry rooting rate comparatively conventional medium improves 8.9-10.5%; stem apex survival rate improves 7.8-9.6%; Quality of Seedlings significantly improves, and leaf is green straight and upright.
Should be understood that, above embodiment is only not used in for illustration of the present invention and limits the scope of the invention, after having read the present invention, the amendment of those skilled in the art to the various equivalent form of value of the present invention has all fallen within the application's claims limited range.
Claims (2)
1. a culture media composition for Strawberry tissue culture, is characterized in that comprising basal medium, inducing culture and expansion breeding culture medium: each component and contained weight as follows:
(1) basal medium: 1/4 improvement MS, gel 3-5g/L, pH is adjusted to 5-6;
(2) induced bud differential medium: improvement MS, gel 6-8g/L, hormone, pH is adjusted to 5.5-6;
(3) root induction medium: 1/2 improvement MS, gel 4-6g/L, hormone, pH is adjusted to 5.5-6;
(4) expand breeding culture medium: improvement MS, gel 6-8g/L, hormone, pH is adjusted to 5.5-6;
The solvent of the MS medium of described improvement is water, and composition comprises macroelement, trace element, molysite and organic matter, concrete component and corresponding concentration as follows:
Wherein macroelement comprises potassium nitrate 1700mg/L, ammonium nitrate 1600mg/L, epsom salt 300mg/L, anhydrous potassium dihydrogenphosphate 150mg/L, calcium chloride dihydrate 450mg/L and disodium ethylene diamine tetraacetate 37mg/L;
Its medium trace element comprises four water manganese sulphate 23.1mg/L, zinc sulphate 9mg/L, boric acid 5.9mg/L, potassium iodide 0.7mg/L, sodium molybdate 0.2mg/L, copper sulphate 0.2mg/L and cobalt oxide 0.03mg/L;
Wherein molysite is ferrous sulfate heptahydrate 26.5mg/L;
Wherein organic matter comprises inositol 100mg/L, glycine 3mg/L, nicotinic acid 0.5mg/L, sucrose 35g/L and nicotinic acid pyridoxine 0.5mg/L;
Wherein in induced bud differential medium, hormone is 2,4-D0.3mg/L and 6BA0.3mg/L; In root induction medium, hormone is NAA0.2mg/L and 6BA0.2mg/L; Expanding hormone in breeding culture medium is 2,4-D0.2mg/L, NAA0.1mg/L and 6BA0.2mg/L.
2. the culture media composition of a kind of Strawberry tissue culture according to claim 1, is characterized in that: described gel is the mixture of agar and gellan gum, and mass ratio is 3:1.
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| US10785920B2 (en) * | 2016-10-14 | 2020-09-29 | Sunshine Horticulture Co., Ltd. | Method of cultivating plant in transparent sealed container and base used therefor |
| CN117305217B (en) * | 2023-10-24 | 2024-08-06 | 临沂市农业科学院 | Optimized stem tip tissue culture medium, culture method and application |
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| CN102893869B (en) * | 2012-10-22 | 2013-10-16 | 浙江省农业科学院 | Root tip detoxification and rapid propagation technology of strawberries |
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Effective date of registration: 20211026 Address after: 750000 group 3, Hongxi village, Hongguang Town, Helan County, Yinchuan City, Ningxia Hui Autonomous Region Patentee after: He Cong Address before: 225300 No.25 Chunhui Road, Zhangguo Town, Xinghua City, Taizhou City, Jiangsu Province Patentee before: Xinghua Lingyi science and Technology Consulting Service Co.,Ltd. |
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