CN103424547B - Liquid detection cup and method for manufacturing same - Google Patents
Liquid detection cup and method for manufacturing same Download PDFInfo
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- CN103424547B CN103424547B CN201310357045.XA CN201310357045A CN103424547B CN 103424547 B CN103424547 B CN 103424547B CN 201310357045 A CN201310357045 A CN 201310357045A CN 103424547 B CN103424547 B CN 103424547B
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Abstract
The invention discloses a liquid detection cup and a method for manufacturing the same, and belongs to the technical field of liquid detection. The liquid detection cup comprises a cup, a test strip clamp plate and cover films; the test strip clamp plate is arranged in the cup, test strip slots used for accommodating test strips are formed in the test strip clamp plate, and the cover films cover opening surfaces of the test strip slots; through holes are formed in the cover films corresponding to the test strip slots, and the distance from the center of each through hole to the edge of the bottom of the corresponding test strip slot is 1/30-1/2 of the height of the test strip slot. The liquid detection cup and the method have the advantages that air inside the test strips can be properly released by the aid of the through holes under the condition that chromatography reaction of the test strips is assuredly normally carried out, the purpose of relieving the pressure is achieved, accordingly, a chromatography effect can be sufficiently and effectively realized, and excellent test results can be obtained.
Description
Technical field
The present invention relates to technical field of liquid detection, particularly relate to a kind of liquid detection cup and preparation method thereof.
Background technology
Existing detection urine cup can be used for the joint-detection of entry, wall of cup is provided with test strip clamp plate, this test strip clamp plate is provided with the test paper slot placing test strips, and the test strips of test is embedded in this test paper slot and uses (namely the size of test strips and the size of test paper slot match).When needs detect, giving detecting urine cup the patient needing to detect, allowing it fill urine voluntarily, and patient often has little understanding to the structure of test strips, principle, when filling urine, very likely urine can be installed to more than MAX line, cause test strips to lose efficacy, cannot detection be completed.
In order to solve the problem, people are through research and development, improvement has been made to this detection urine cup, in test strips in the face of liquid one side has set up transparent film layer, this transparent film layer is covered in test strips surface, thus test strips is all covered, or cover major part, only leave bottom part sample application zone and directly contact with liquid.Therefore, when detecting cup built with liquid, test strips top seal is got up by transparent film layer, and only leaving sample application zone directly can contact with liquid, thus ELISA test strip will be made invalid more than MAX line due to liquid, and then guarantees the effective loading of test strips, normal reaction.
But we find, in chromatography process of being everlasting, the chromatography of liquid in test strips is creeped may be subject to hindering and slack-offly even to be stopped, and causes normally measuring.
Summary of the invention
Based on this, the object of the invention is to the defect overcoming prior art, a kind of liquid detection cup is provided, adopt this detection cup to carry out fluid sample detection, carrying out smoothly of chromatography process can be guaranteed, obtain and measure effect preferably.
For achieving the above object, the present invention takes following technical scheme:
A kind of liquid detection cup, comprises cup, test strip clamp plate and coverlay; Described test strip clamp plate is placed in cup, which is provided with the test paper slot for placing test strips, and described coverlay covers test paper slot opening surface, sealing test paper slot inner space; This through hole is located on the coverlay corresponding to test paper slot, and the length of this through hole centre distance test paper slot bottom margin is the 1/30-1/2 of test paper slot height.
The present invention is found by research, simple coating epiphragma all or the test strips that covers of major part be unfavorable for that chromatography reacts, its reason is as follows: when test strips loading just starts, certain gap is there is between test strips surface and covering hyaline membrane, in test strips, gas extrudes by environmental liquids, part can be discharged by this gap, but undergoes rapid expansion after sample application zone water suction, the test strips sample application zone of coverlay and imbibition fits tightly, this gap is filled disappearance soon, in test strips, gas cannot be discharged from here again, so far, test strips is completely sealed.And be occur by capillary action because test strips chromatography reacts, as gaseous tension in test strips is greater than the pulling force of capillary action generation, if namely capillary action pulling force cannot overcome the resistance that in test strips, gas produces, the chromatography effect of test strips will be hindered, and normally cannot carry out chromatography.
And in the process of liquid chromatography in test strips, need to get over label district from sample application zone, then in results display area display result, this process is undertaken by capillary pulling force.And once test strips inside is closed, the excessive gas remained in test strips cannot be discharged, certain pressure intensity is formed in test strips inside, need to resist this internal pressure in the process that liquid chromatography in test strips is creeped, once pressure is greater than the pulling force that capillary action produces, chromatography effect will be hindered or cannot normally be carried out.From such principle, the present invention has investigated multiple release mode, and through a large amount of experimental studies, final selection arranges by the bottom corresponding to test strips on coverlay the object that through hole reaches release.
The present invention also provides a kind of liquid detection cup, comprises cup, test strip clamp plate, test strips and coverlay; Described test strip clamp plate is placed in cup, which is provided with test paper slot, and described test strips is positioned in described test paper slot, and described coverlay covers the detection faces of test strips, sealing test strips; Described coverlay is provided with through hole, and this through hole is located on the coverlay corresponding to test paper slot, and the length of this through hole centre distance test paper slot bottom margin is the 1/30-1/2 of test paper slot height.
Wherein in an embodiment, described through hole is located on the coverlay below corresponding to test strips label pad.The present invention finds after deliberation, and the setting position of through hole is also very crucial.When the position of through hole is higher, during as being arranged on the thieving paper position corresponding to the test strips sample application zone other end, when in detection cup, liquid levels is lower than this through hole, in test strips, excessive gas is discharged by this through hole, and chromatography reaction still can normally be carried out; And when liquid level in cup is higher than this through hole, in a flash incipient, a gas column is formed between the test strips that coating epiphragma seals and coverlay, through hole is then equivalent to the opening in this gas column, and at this moment, this gas column and outside liquid form huge pressure difference, gas column internal gas pressure is far smaller than outside liquid pressure, in order to reach inside and outside pressure equilibrium, outside liquid will enter in this gas column by this through hole under the pressure of this pressure difference, and leaching is completely coated to the test strips that epiphragma seals rapidly, causes test strips invalid.
When through hole being located at corresponding on the coverlay (namely corresponding to sample application zone place) below test strips bottom indicia pad, through hole is in the bottom of test strips.Liquid is in test strips sample application zone loading process, expand gradually after sample application zone water suction, but when also not fitting completely with covering hyaline membrane, there is gap between test strips sample application zone and covering hyaline membrane, in test strips, gas is discharged by this gap and through hole; And when sample application zone expand into completely laminating cover hyaline membrane time, gap is sealed, and the gas in test strips cannot be discharged from here again.When liquid comes lead to the hole site, the test strips part yet imbibition gradually of through hole, is not also expanding into hyaline membrane of fitting completely, and when through hole is not blocked completely, in test strips, gas still can be discharged from this through hole.When liquid level in cup is lower than through hole position, Exhaust Gas naked eyes are invisible; When liquid level in cup is higher than through hole place height, liquid discharges macroscopic bubble by through hole.When the test strips of through hole continue to expand into fit coverlay completely time, gas can not be discharged from through hole again, and now, test strips is in complete sealing state, and test strips will carry out chromatography by capillary force.At this moment may still there is a certain amount of gas in test strips, but after being discharged by the place that above-mentioned two places can be vented, in test strips, pressure is moderate, and chromatography effect can be carried out smoothly.
The reasonable setting of this through hole can assist the discharge of gas in test strips, after the test strips at coverlay bottom margin place expands, exhausr port is blocked, when making that in test strips, excessive gas cannot be discharged from here again, gas is also discharged by through hole, until test strips expands block through hole, thus extend gas efflux time in test strips, reduce pressure in test strips, guarantee that chromatography effect is normally carried out.
Wherein in an embodiment, the length of described coverlay bottom edge distance test strips bottom margin is the 1/6-5/6 of sample application zone length in test strips.And the bottom edge reference position of coverlay and through hole setting position very crucial to the quality of chromatographic effect, coverlay is allowed to cover a part of sample application zone, instead of test strips is covered or only covering by label pad district completely, object makes liquid have a buffer action when test strips loading, liquid can get over labeling pad by chromatography effect, then in viewing area display result.As test strips covered completely, be then difficult to the loading that assures success; As coverlay only overlay marks pad district and with upper part, then the sample application zone of steeping in liquid is oversize, and liquid directly may break through label pad, rushes sample phenomenon, and causing chromatography to react can not normally carry out.
Wherein in an embodiment, described through hole center is 7:1-3:1 with the Distance geometry through hole center of test strips bottom margin and the distance ratio of label pad lower limb.When through hole is positioned at this height, the inside and outside pressure difference of test strips is best, can obtain best chromatographic effect.
Wherein in an embodiment, the diameter of described through hole or the length of side are the 1/3-1/2 of test strips width.As through hole is too large, liquid is easily directly entered in test strips by this through hole, and through hole does not have scavenging action, and be equivalent to coverlay from through hole, through hole performs practically no function.And if through hole arranges too little, when liquid is in test strips during loading, sample application zone undergoes rapid expansion, soon through hole is sealed, the gas flow of discharging from this through hole is quite few, be unfavorable for the release of pressure in test strips, the gas remaining in test strips inside is more, is unfavorable for that in test strips, chromatography sufficient reacting carries out.Therefore, after experiment repeatedly, determine when the diameter of through hole or the length of side are the 1/3-1/2 of test strips width, best results.
Wherein in an embodiment, described coverlay is hyaline membrane; Described test strip clamp plate is arranged along cup circumference, and described test paper slot is located at test strip clamp plate towards cup side, and described cup is transparent cup, is convenient to observe read result.
Wherein in an embodiment, described test strip clamp plate is connected with cup is detachable.Produce, easy to assembly.
Wherein in an embodiment, also comprise bowl cover, described bowl cover and cup threaded engagement; O-ring seal is provided with at the bottom of described bowl cover.Coordinate the use of bowl cover, the problem detecting fluid seepage in cup can be avoided.The use of O-ring seal, improves the leakproofness between cup and bowl cover.Described O-ring seal is preferably made up of silica gel.Silica gel material is not easily aging, good leak tightness, and it is inert material, can not react, affect testing result with the liquid detected in cup.And when tested liquid needs to be sent to secondary detection, this is provided with and is beneficial to liquid in sealing preservation cup, avoids liquid in transport process owing to being inverted, inclination is leaked, and affects censorship, contaminated environment.
The present invention also provides a kind of method for making of liquid detection cup, comprises the following steps:
1) with the detection faces of test strips towards outside test paper slot, test strips is positioned in the test paper slot in test strip clamp plate;
2) be covered in above-mentioned ELISA test strip face with the coverlay being provided with through hole, test strips be sealed in the confined space of coverlay and test paper slot composition, and make the position of described through hole correspond to test strips label pad with lower part;
3) test strips assembled by above-mentioned steps, coverlay and test strip clamp plate are put into the cup detecting cup, to obtain final product.
By the liquid detection cup that said method is obtained, carrying out smoothly of chromatography process can be guaranteed, obtain and measure effect preferably.
Compared with prior art, the present invention has following beneficial effect:
Liquid detection cup of the present invention, coverlay corresponds to test strips label pad through hole is set with lower part, the reasonable setting of this through hole can assist the discharge of gas in test strips, after the test strips at coverlay bottom margin place expands, place exhausr port is blocked, when making that in test strips, excessive gas cannot be discharged from here, gas also can continue through described through hole and discharge, until test strips expands block through hole, thus further reduce pressure in test strips, guarantee that chromatography effect is normally carried out.
And the present invention is designed by repetition test and verifies, finally determine the parameters such as best coverlay reference position, lead to the hole site and size, best test result can be obtained with the chromatography process of optimum.
By the liquid detection cup that making liquid detection cup method of the present invention is obtained, carrying out smoothly of chromatography process can be guaranteed, obtain and measure effect preferably.
Accompanying drawing explanation
Fig. 1 is the detection cup cup of embodiment 1, test strip clamp plate and cup lid structure schematic diagram;
Fig. 2 is test strip clamp plate structural representation in Fig. 1;
Fig. 3 is test paper slot floor map in test strip clamp plate in Fig. 1;
Fig. 4 is embodiment 1 covered film structure schematic diagram;
Fig. 5 is embodiment 1 test strips structural representation;
Fig. 6 is embodiment 1 test strip clamp plate, test strips and coverlay assembling schematic diagram;
Fig. 7 is that Fig. 6 has assembled schematic diagram;
Fig. 8 is Fig. 7 front view;
Fig. 9 is the cup of the detection cup of embodiment 2, test strip clamp plate and cup lid structure schematic diagram.
Wherein: 1. cup; 2. test strip clamp plate; 21. test paper slots; 3. test strips; 31. sample application zone; 32. label pad districts; 33. results display area; 331. detection zone; 332. control zone; 34. thieving papers; 4. coverlay; 41. through holes; 5. bowl cover; 51. O-ring seals.
Embodiment
The present invention is described in detail below in conjunction with the drawings and specific embodiments.
Embodiment 1
A kind of liquid detection cup, as shown in Fig. 1, Fig. 6, comprises cup 1, test strip clamp plate 2, test strips 3 and coverlay 4; Described test strip clamp plate 2 is placed in cup 1, which is provided with test paper slot 21, and described test strips 3 is positioned in described test paper slot 21, as seen in figs. 5-6, and the wide 3mm of described test strips 3, long 60mm; Sample application zone 31 and label pad district 32 long 20mm altogether, wherein, sample application zone 12mm, label pad district 8mm; Results display area 33 is 10mm, results display area 33 is provided with again detection zone 331 and control zone 332; Thieving paper 34 part 22mm.Described coverlay 4 is hyaline membrane, and as shown in Figure 4, coverlay 4 is covered in the detection faces of test strips 2, sealing test strips; And dash area single spreading in the diagram, in order to paste with test strip clamp plate 2, be adhesively fixed coverlay 4.The length of coverlay 4 bottom edge distance test strips 3 bottom margin is 8mm; Described coverlay 4 corresponds to test strips 2 label pad and be provided with through hole 41 with lower part, as shown in figs 6-8, described through hole 41 centre distance label pad district 32 lower limb 2mm, this through hole 41 is circular, and its diameter is 1.25mm.Be understandable that, this through hole 41 shape also can be square, and any shape such as triangle, as long as can by gas.
As Figure 1-3, described test strip clamp plate 2 is arranged along cup 1 circumference, and described test paper slot 21 is located at test strip clamp plate 2 towards cup 1 side, and described cup is transparent cup.Be convenient to observe and read result.
Described test strip clamp plate 2 is connected with cup 1 is detachable.Produce, easy to assembly.Be square bottom described cup 1.
This detection cup also comprises bowl cover 5, described bowl cover 5 and cup 1 threaded engagement; O-ring seal 51 is provided with at the bottom of described bowl cover.Coordinate the use of bowl cover 5, the problem detecting fluid seepage in cup can be avoided.The use of O-ring seal, improves the leakproofness between cup and bowl cover.Described O-ring seal is preferably made up of silica gel.Silica gel material is not easily aging, good leak tightness, and it is inert material, can not react, affect testing result with the liquid detected in cup.And when tested liquid needs to be sent to secondary detection, this is provided with and is beneficial to liquid in sealing preservation cup, avoids liquid in transport process owing to being inverted, inclination is leaked, and affects censorship, contaminated environment.
The method for making of the liquid detection cup of the present embodiment is:
1) with the detection faces of test strips towards outside test paper slot, test strips is positioned in the test paper slot in test strip clamp plate, fixes;
2) above-mentioned ELISA test strip face is covered in the coverlay being provided with through hole, and at coverlay and test strip clamp plate contact portion gluing, in order to paste with test strip clamp plate, thus test strips be sealed in the confined space of coverlay and test paper slot composition, and the position of described through hole is made to correspond to test strips label pad with lower part;
3) test strips assembled by above-mentioned steps, coverlay and test strip clamp plate are put into the cup detecting cup, to obtain final product.
Embodiment 2
As shown in Figure 9, the liquid detection cup of the present embodiment is substantially identical with the detection cup of embodiment 1, and the key distinction is:
The length of coverlay bottom edge distance test strips bottom margin is 6mm; Described coverlay corresponds to test strips label pad and be provided with through hole with lower part, described through hole centre distance label pad district lower limb 3mm, this through-hole diameter is 1mm.
Described bottom of cup is circular.
Embodiment 3
The liquid detection cup of the present embodiment is substantially identical with the detection cup of embodiment 1, and the key distinction is:
The length of coverlay bottom edge distance test strips bottom margin is 9mm; Described coverlay corresponds to test strips label pad and be provided with through hole with lower part, described through hole centre distance label pad district lower limb 1.5mm, this through-hole diameter is 1.5mm.
Embodiment 4
The liquid detection cup of the present embodiment is substantially identical with the detection cup of embodiment 1, and the key distinction is:
Application of sample head of district 15mm, the length of coverlay bottom edge distance test strips bottom margin is 10mm; Described coverlay corresponds to test strips label pad and be provided with through hole with lower part, described through hole centre distance label pad district lower limb 2.5mm, this through-hole diameter is 1.4mm.
Embodiment 5 comparative experimental example
Adopt the detection cup of embodiment 1-4, coverlay establish the detection cup of through hole to carry out chromatography test in detection cup that through hole is located at thieving paper position and prior art.
Wherein: the detection cup that through hole is located at thieving paper position is substantially identical with the detection cup of embodiment 1, and the key distinction is, the length of coverlay bottom edge distance test strips bottom margin is 17mm; Described coverlay corresponds to test strips thieving paper position and be provided with through hole, described through hole centre distance test strips tip edge 5mm.
This experiment is by being divided into 500 parts to gathering the sample come, employing specification, the ketamine test item test strips that type is identical are tested, and liquid in detection cup is all filled into the results display area do not had in test strips, investigate its effect, experimental result is as shown in table 1 below.
Can be found out by the above results, use the detection cup not establishing through hole in prior art, because test strips internal pressure is excessive, cause loading time needed for the chromatography effect of part test strips long, even cannot successful loading, namely tracer liquid can not climb precalculated position, and control zone cannot be made to produce respective change, and loading rate is lower.And through hole is located at thieving paper part, because liquid climbs too fast, cause determinand also not have and the respective markers thing reaction in labeling pad, or liquid break through detection zone fast, there is no time enough and the antigen/antibody reaction being coated on detection zone, cause the sample rate of rushing of generation higher.And the detection cup of embodiment of the present invention 1-4, owing to have employed the parameters such as good coverlay reference position, lead to the hole site and size, its loading rate is higher, and it is little to rush sample rate, detects duration moderate, has best Detection results.
The above embodiment only have expressed several embodiment of the present invention, and it describes comparatively concrete and detailed, but therefore can not be interpreted as the restriction to the scope of the claims of the present invention.It should be pointed out that for the person of ordinary skill of the art, without departing from the inventive concept of the premise, can also make some distortion and improvement, these all belong to protection scope of the present invention.Therefore, the protection domain of patent of the present invention should be as the criterion with claims.
Claims (10)
1. a liquid detection cup, comprises cup, test strip clamp plate and coverlay; Described test strip clamp plate is placed in cup, which is provided with the test paper slot for placing test strips, and described coverlay covers test paper slot opening surface; It is characterized in that: described coverlay is provided with through hole, this through hole is located on the coverlay corresponding to test paper slot, and the length of this through hole centre distance test paper slot bottom margin is the 1/30-1/2 of test paper slot height.
2. a liquid detection cup, comprises cup, test strip clamp plate, test strips and coverlay; Described test strip clamp plate is placed in cup, which is provided with test paper slot, and described test strips is positioned in described test paper slot, and described coverlay covers the detection faces of test strips; It is characterized in that: described coverlay is provided with through hole, this through hole is located on the coverlay corresponding to test paper slot, and the length of this through hole centre distance test paper slot bottom margin is the 1/30-1/2 of test paper slot height.
3. liquid detection cup according to claim 2, is characterized in that, described through hole is located on the coverlay below corresponding to test strips label pad.
4. liquid detection cup according to claim 2, is characterized in that, the length of described coverlay bottom edge distance test strips bottom margin is the 1/6-5/6 of sample application zone length in test strips.
5. liquid detection cup according to claim 2, is characterized in that, described through hole center is 7:1-3:1 with the Distance geometry through hole center of test strips bottom margin and the distance ratio of label pad lower limb.
6. liquid detection cup according to claim 2, is characterized in that, the diameter of described through hole or the length of side are the 1/3-1/2 of test strips width.
7. liquid detection cup according to claim 2, is characterized in that, described coverlay is hyaline membrane; Described test strip clamp plate is arranged along cup circumference, and described test paper slot is located at test strip clamp plate towards cup side, and described cup is transparent cup.
8. liquid detection cup according to claim 2, is characterized in that, described test strip clamp plate is connected with cup is detachable.
9. liquid detection cup according to claim 2, is characterized in that, also comprises bowl cover, described bowl cover and cup threaded engagement; O-ring seal is provided with at the bottom of described bowl cover.
10. a method for making for liquid detection cup, is characterized in that, comprises the following steps:
1) with the detection faces of test strips towards outside test paper slot, test strips is positioned in the test paper slot in test strip clamp plate;
2) be covered in above-mentioned ELISA test strip face with the coverlay being provided with through hole, test strips be sealed in the confined space of coverlay and test paper slot composition, and make the position of described through hole correspond to test strips label pad with lower part;
3) test strips assembled by above-mentioned steps, coverlay and test strip clamp plate are put into the cup detecting cup, to obtain final product.
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| CN201310357045.XA CN103424547B (en) | 2013-08-15 | 2013-08-15 | Liquid detection cup and method for manufacturing same |
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| CN201310357045.XA CN103424547B (en) | 2013-08-15 | 2013-08-15 | Liquid detection cup and method for manufacturing same |
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| CN103424547B true CN103424547B (en) | 2015-02-25 |
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| JP6538160B2 (en) * | 2014-06-12 | 2019-07-03 | スィケリウプ エッセ．エッレ．エッレ． | Container for the determination of urinary analytes and / or chemical physical parameters and measurement of urinary sediment, and method of sufficient urine analysis using this container |
| CN104950108A (en) * | 2015-06-26 | 2015-09-30 | 广州万孚生物技术股份有限公司 | Liquid direction cup and detection test paper strip in liquid detection cup |
| CN105044102B (en) * | 2015-08-10 | 2019-02-01 | 杭州康永生物技术有限公司 | Test strips inserted sheet, manufacturing method and detection device for detection cup |
| CN106645663B (en) * | 2017-01-22 | 2023-07-18 | 英科新创(厦门)科技股份有限公司 | Anti-pollution vertical chromatography detection device |
| CN107271334B (en) * | 2017-05-25 | 2019-09-17 | 东莞长盈精密技术有限公司 | Film liquid detection system |
| CN109085159B (en) * | 2017-08-21 | 2024-09-06 | 上海荒岛科技有限公司 | Base plate and detection card |
| CN109085348B (en) * | 2017-08-21 | 2019-07-23 | 上海荒岛科技有限公司 | A kind of method for analyzing body fluid |
| CN109342673A (en) * | 2018-12-14 | 2019-02-15 | 深圳市心月生物科技有限公司 | a milk cup |
| CN110275029B (en) * | 2019-07-30 | 2024-09-10 | 南通伊仕生物技术股份有限公司 | Semi-quantitative detection test paper and reagent cup for human chorionic gonadotrophin, and preparation method and application thereof |
| CN210953503U (en) * | 2019-09-06 | 2020-07-07 | 杭州奥泰生物技术股份有限公司 | Micropore even pressure prevents capillary fast sampling fluid detection device |
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| CN103424547A (en) | 2013-12-04 |
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