CN103421684A - Gas-producing microbe rapid examining tube - Google Patents
Gas-producing microbe rapid examining tube Download PDFInfo
- Publication number
- CN103421684A CN103421684A CN2012101551765A CN201210155176A CN103421684A CN 103421684 A CN103421684 A CN 103421684A CN 2012101551765 A CN2012101551765 A CN 2012101551765A CN 201210155176 A CN201210155176 A CN 201210155176A CN 103421684 A CN103421684 A CN 103421684A
- Authority
- CN
- China
- Prior art keywords
- pipe
- tube
- lucifuge
- outer tube
- aerogens
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Granted
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- 238000012360 testing method Methods 0.000 claims description 37
- 229920003023 plastic Polymers 0.000 claims description 8
- 238000007789 sealing Methods 0.000 claims description 6
- 239000004033 plastic Substances 0.000 claims description 5
- 229920005479 Lucite® Polymers 0.000 claims description 2
- 239000004926 polymethyl methacrylate Substances 0.000 claims description 2
- 239000012780 transparent material Substances 0.000 claims 1
- 210000005239 tubule Anatomy 0.000 description 13
- 238000000034 method Methods 0.000 description 12
- 239000000203 mixture Substances 0.000 description 11
- 239000000463 material Substances 0.000 description 9
- 230000001954 sterilising effect Effects 0.000 description 7
- 238000002474 experimental method Methods 0.000 description 4
- 238000011160 research Methods 0.000 description 4
- 238000010586 diagram Methods 0.000 description 2
- 239000012467 final product Substances 0.000 description 2
- 238000007689 inspection Methods 0.000 description 2
- 244000005700 microbiome Species 0.000 description 2
- 235000015097 nutrients Nutrition 0.000 description 2
- 241001466460 Alveolata Species 0.000 description 1
- 241000193468 Clostridium perfringens Species 0.000 description 1
- GUBGYTABKSRVRQ-QKKXKWKRSA-N Lactose Natural products OC[C@H]1O[C@@H](O[C@H]2[C@H](O)[C@@H](O)C(O)O[C@@H]2CO)[C@H](O)[C@@H](O)[C@H]1O GUBGYTABKSRVRQ-QKKXKWKRSA-N 0.000 description 1
- 240000004808 Saccharomyces cerevisiae Species 0.000 description 1
- 241000973497 Siphonognathus argyrophanes Species 0.000 description 1
- 230000015572 biosynthetic process Effects 0.000 description 1
- 239000003153 chemical reaction reagent Substances 0.000 description 1
- 238000010276 construction Methods 0.000 description 1
- 230000007812 deficiency Effects 0.000 description 1
- 239000003814 drug Substances 0.000 description 1
- 238000005516 engineering process Methods 0.000 description 1
- 238000009434 installation Methods 0.000 description 1
- 239000008101 lactose Substances 0.000 description 1
- 238000012544 monitoring process Methods 0.000 description 1
- 238000012856 packing Methods 0.000 description 1
- 238000004321 preservation Methods 0.000 description 1
- 210000003813 thumb Anatomy 0.000 description 1
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- Devices For Use In Laboratory Experiments (AREA)
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
The invention discloses a gas-producing microbe rapid examining tube. The tube comprise an external tube, whose low end is enclosed, and a sealing cover, which is arranged on the opening of the external tube, the sealing cover is downward provided with a connecting tube, which is used for letting a lucifugal tube go through; the upper end of the lucifugal tube is enclosed, the lower end of the lucifugal tube is open, the external wall of the lucifugal tube hermetically and slidingly cooperates with the inner wall of the connecting tube; the lower end of the connecting tube is provided with a lucifugal plug, which can slidingly cooperate with the connecting tube; an inner tube with one end open is fixedly arranged in the external tube, the open end is downward arranged, a gap is saved between the open end and the bottom of the external tube, and a gap is saved between the external wall of the inner tube and the inner wall of the external tube. The examining tube is capable of prolonging the expiration date of culture medium, and has the advantages of low cost, simple operation and precise observation on gas production situation.
Description
Technical field
The present invention relates to a kind of equipment that Institute of Micro-biology uses that detects, especially relate to the test tube that a kind of aerogens is cultivated and check is used.
Background technology
Coliform means a group energy ferment lactose, produces sour aerogenesis, aerobic and amphimicrobian Gram-negative bactacin, be the monitoring of hygiene one of must the micrometer biology.The units such as food and medicine Surveillance Authority, customs, commodity inspection, food enterprise and pharmaceutical factory often need to check coliform, and the current method of inspection has ordinary method and the Rapid examination method of GB regulation.In the recurrence ferment test of ordinary method, first prepare the BGLG substratum, divide and install in the test tube of Du Shi tubule, then culture is inoculated in this test tube and cultivates, by the Du Shi tubule, observe the aerogenesis situation.This method need to be prepared the complicated processes such as substratum, packing and sterilizing, and most reviewer often runs into after sterilizing alveolate problem in the Du Shi tubule, makes culture tube not use, and has both lost time and reagent, increases again reviewer's workload.Rapid examination method mainly contains coliform fast detector and Quick detecting paper sheet for colibacillus group, although the Rapid examination method required time is short, fast detector is expensive, and middle-size and small-size unit is difficult to configuration; The quick test scraps of paper can only be observed and produce sour situation, can't observe the aerogenesis situation.
In addition, the aerogenesis situation of the aerogenesis type microorganisms such as some scientific research institution's research clostridium perfringens and yeast, generally the Du Shi tubule is inverted in test tube, observe the intratubular gas of Du Shi, and place the Du Shi tubule, scientific research personnel's operating skill be there are certain requirements, if it is improper to place, can make has bubble in the Du Shi tubule, affects observations.
Summary of the invention
The present invention is directed to the deficiencies in the prior art, provide a kind of observation aerogenesis situation accurately, conveniently, and aerogens quick test pipe simple to operate.
For achieving the above object, the present invention can take following technical proposals: a kind of aerogens quick test pipe, and comprise the outer tube of lower end closed and be located at the capping at outer tube mouth of pipe place, described capping upper surface is to having the pipe connecting passed for a lucifuge pipe; The upper end closed of described lucifuge pipe, lower ending opening, the outer wall of lucifuge pipe coordinates with the inwall slidable sealing of pipe connecting; The lower end of pipe connecting is provided with a lucifuge plug, and this lucifuge plug slidably coordinates with pipe connecting; Be installed with the inner tube of an end opening in described outer tube, down, and the bottom of opening end and outer tube leaves gap to its opening end, between the outer wall of inner tube and the inwall of outer tube, leaves gap.
As preferably, the outer wall of described lucifuge pipe is provided with flange collar, and the lucifuge pipe coordinates with the pipe connecting inner wall sealing by this flange collar.
As preferably, the outer annular projection that is arranged with of described lucifuge pipe.
As preferably, the outer collar that is arranged with of described lucifuge pipe, this collar is between the upper surface of annular projection and capping.
As preferably, the side of described capping radially is provided with flange, along flange inner to having press section.
As preferably, described inner tube is by arranging union lever and outer tube is connected.
As preferably, the mouth of pipe place of outer tube is located in described capping by hinged mode.By hinged mode, convenient opening, can realize one-handed performance.
As preferably, described outer tube, inner tube are lucite pipe.
As preferably, described lucifuge pipe, lucifuge plug are plastics tubing.
Aerogens quick test pipe of the present invention, upper end is provided with the lucifuge pipe, the lucifuge pipe is through capping, seal cartridge is in pipe connecting, can in pipe connecting, slide up and down, be plugged with a lucifuge plug in the pipe connecting lower end, this lucifuge plug and lucifuge pipe are opaque plastics tubing and make, and can deposit and see the labile composition of light.Between lucifuge plug and pipe connecting, be also slidably to coordinate, when pressing lucifuge pipe upper end, the lucifuge plug will be ejected by the lucifuge pipe, be that the lucifuge plug splits away off from pipe connecting, material in the lucifuge pipe is just dropped in the substratum in outer tube like this, the lucifuge plug also can fall into substratum, but the lucifuge plug does not affect experiment through sterilising treatment.
Before experiment, first, by seeing in substratum that the labile composition of light adds in the lucifuge pipe, other compositions of substratum are added in outer tube, standby after sterilizing.During experiment, press the upper end of lucifuge pipe, the material in the lucifuge pipe is dropped in outer tube, shakes up and can be tested.As: in the recurrence ferment test of check coliform, open capping, can be in advance bilein etc. be shown in to the labile composition of light adds in the lucifuge pipe, then lucifuge plug beyond the Great Wall, push lucifuge pipe and lucifuge plug in pipe connecting; Again other compositions of BGLB substratum are added to outer tube, cover capping, save backup after sterilizing.During use, take away collar, press the lucifuge pipe, make the lucifuge plug be ejected pipe connecting by the lucifuge pipe, in lucifuge plug and lucifuge pipe, material falls into outer tube, after shaking up, can open on the other hand capping, adds on the other hand and treats the test sample cultivation, observe Bubble formation situation in nutrient solution colour-change and inner tube, with this, judge to treat in test sample whether have coliform to exist.
In prior art, general substratum needs matching while using, uses inconvenience, and cultivates test tube used and stopper separate, easily pollutes, and opens also inconvenience.And capping of the present invention and body are opened conveniently, can realize one-handed performance, and add in advance the lucifuge composition, during use, only need press the lucifuge pipe can carry out test experiments, simple operation, has reduced contaminated probability; The lucifuge pipe can be deposited in advance and see the labile composition of light, while by the time testing, just the lucifuge material is dropped into to substratum, and the mode of taking substratum and lucifuge composition separately to preserve makes the quality guaranteed period of substratum extend.
Moreover, while observing microorganism aerogenesis situation in prior art, need to place in addition a tubule and observed, if place improper, can make in tubule to have bubble, affect observations, and inner tube of the present invention and outer tube are structure as a whole, without other placement, easy to operate, and also observations is more accurate.
In sum, the present invention has the following advantages:
1. the present invention does not need the proofer to prepare the set-up procedures such as substratum and sterilizing, can directly add and treat the test sample quick test, convenient, has greatly reduced contaminated probability;
2. lucifuge pipe of the present invention can be deposited in advance and see the labile composition of light, makes the quality guaranteed period of substratum extend;
3. cost of the present invention is lower, easy and simple to handle, and the aerogenesis situation is observed accurately.
The accompanying drawing explanation
Fig. 1 is structural representation of the present invention.
Fig. 2 is open mode schematic diagram of the present invention.
Fig. 3 is end construction schematic diagram of the present invention.
Embodiment
In order to make those skilled in the art person better understand the present invention program, below in conjunction with the accompanying drawing in the embodiment of the present invention, technical scheme in the embodiment of the present invention is carried out to clear, complete description, obviously, described embodiment is only a part of embodiment of the present invention, rather than whole embodiment.Embodiment based in the present invention, those of ordinary skills, not making under the creative work prerequisite the every other embodiment obtained, should belong to the scope of protection of the invention.
As Fig. 1, shown in Fig. 2, a kind of aerogens quick test pipe, comprise transparent plastics outer tube 1, the lucifuge pipe 5 that transparent plastics capping 3 and opaque plastics are made, the lower end closed of outer tube 1, upper end open, one side of capping 3 and the mouth of pipe of outer tube 1 are hinged, hinged mode can more conveniently be opened capping, the free side of capping 3 and outer tube 1 fasten, capping 3 upper surfaces have a through hole, extend a pipe connecting 4 downwards along this through hole, the end face of the upper end of this pipe connecting 4 and capping 3, during installation, lucifuge pipe 5 inserts this pipe connecting 4, the outer wall of lucifuge pipe 7 is that slidable sealing coordinates with the inwall of pipe connecting 4, only have under external force, lucifuge pipe 5 can slide up and down along the inwall of pipe connecting 4, when there is no External Force Acting, lucifuge pipe 5 is that to be fixed on pipe connecting 4 interior actionless, and is processed with 3 flange collars 7 at the outer wall of lucifuge pipe 5, can increase frictional force, be conducive to the fixing of lucifuge pipe, also can increase stopping property, be conducive to the preservation of the lucifuge material in the lucifuge pipe, the upper end closed of lucifuge pipe 5, lower ending opening, during use, open capping, will see that the labile composition of light adds in lucifuge pipe 5, and then lucifuge plug 6 beyond the Great Wall, push lucifuge pipe 5 and lucifuge plug 6 in pipe connecting, covers capping and get final product.This lucifuge plug 6 also opaque plastic material is made, and under external force, while pressing the lucifuge pipe, the lucifuge pipe can eject pipe connecting by the lucifuge plug, and the labile material of light of seeing in the lucifuge pipe just falls into outer tube smoothly like this.When there is no external force, the lucifuge plug is sealed in the lucifuge material in the lucifuge pipe.
In order to observe the cultivation situation of aerogens, the inner tube 2 of making at transparent plastics of the interior inversion of outer tube 1, leave certain interval between the inwall of the outer wall of inner tube 2 and outer tube 1, inner tube 2 one end sealings, one end opening, its opening end down, and a segment distance is left in the bottom of opening end and outer tube 1, in the present embodiment, inner tube is placed in to 1/3 place of outer tube, and the substratum in outer tube do not have the top of inner tube, be provided with 4 union levers 21 between the inwall of the outer wall of inner tube 2 and outer tube 1, union lever 21 is fixed on inner tube 2 in outer tube 1 firmly.The inner tube here is equivalent to a Du Shi tubule, while testing in prior art, generally the Du Shi tubule is inverted in test tube, observe the intratubular gas of Du Shi, and place the Du Shi tubule, scientific research personnel's operating skill be there are certain requirements, if it is improper to place, can make has bubble in the Du Shi tubule, affects observations.And, in the present invention, the mode that adopts the Du Shi tubule to be fixedly connected with outer tube, removed above-mentioned trouble from, it is more accurate to make to observe.
While adopting test tube of the present invention to be tested, we can adopt one-handed performance, and left hand is taken test tube, and the right hand is taken transfering loop or liquid-transfering gun to add and treated test sample, can greatly reduce pollution.Prior art adopts stopper to fill in the method for test tube, opens the stopper inconvenience, and easily pollutes nutrient solution.This test tube adopts one-handed performance, both convenient, again vulnerable to pollution not; And, in order to be easy to open capping 3, we radially are provided with a flange 31 in the side of capping 3, along the root of flange 31 to downward-extension one press section 32, during operation, thumb is pinned press section 32, more upwards backs down flange 31, thereby raise capping 3, easy to operate, quick.
Whole by having arrived in pipe connecting 4 in order to prevent lucifuge pipe 5, can lucifuge pipe 5 outer be arranged with a loop projection or several salient points also passable, be generally to establish a loop projection in the end of lucifuge pipe, like this, even overexert, also can be by lucifuge pipe gear in the capping upper end.In the present embodiment, be the outer loop projection 8 that encloses in the upper end of lucifuge pipe.In order to prevent being pressed into because of carelessness the lucifuge pipe in transportation or moving process, cause the material in the lucifuge pipe to be dropped in outer tube, can be at collar 9 of lucifuge pipe outer race, collar 9 is common collar of the prior art, it is fixed on the lucifuge pipe between annular projection 8 and capping 3, during use, pull up collar and get final product, easily.The present invention does not need the proofer to prepare the set-up procedures such as substratum and sterilizing, can directly add and treat the test sample quick test, convenient, has greatly reduced contaminated probability; Lucifuge pipe of the present invention can be deposited in advance and see the labile composition of light, makes the quality guaranteed period of substratum extend; Cost of the present invention is lower, easy and simple to handle, and the aerogenesis situation is observed accurately.
Claims (9)
1. an aerogens quick test pipe comprises the outer tube (1) of lower end closed and is located at the capping (3) at outer tube (1) mouth of pipe place that it is characterized in that: described capping (3) upper surface is to having the pipe connecting (4) passed for a lucifuge pipe (5); The upper end closed of described lucifuge pipe (5), lower ending opening, the outer wall of lucifuge pipe (5) coordinates with the inwall slidable sealing of pipe connecting (4); The lower end of pipe connecting (4) is provided with a lucifuge plug (6), and this lucifuge plug (6) slidably coordinates with pipe connecting (4); Be installed with the inner tube (2) of an end opening in described outer tube (1), down, and the bottom of opening end and outer tube (1) leaves gap to its opening end, between the inwall of the outer wall of inner tube (2) and outer tube (1), leaves gap; Described outer tube (1) is transparent material with inner tube (2).
2. a kind of aerogens quick test pipe according to claim 1, it is characterized in that: the outer wall of described lucifuge pipe (5) is provided with flange collar (7), and lucifuge pipe (5) coordinates with pipe connecting (4) inner wall sealing by this flange collar (7).
3. a kind of aerogens quick test pipe according to claim 2, is characterized in that: the outer annular projection (8) that is arranged with of described lucifuge pipe (5).
4. a kind of aerogens quick test pipe according to claim 3, it is characterized in that: the outer collar (9) that is arranged with of described lucifuge pipe (5), this collar (9) is positioned between the upper surface of described annular projection (8) and capping (3).
5. a kind of aerogens quick test pipe according to claim 4, it is characterized in that: the side of described capping (3) radially is provided with flange (31), inboard to having press section (32) along flange (31).
6. a kind of aerogens quick test pipe according to claim 5 is characterized in that: described inner tube (2) is connected with outer tube (1) by union lever (21) is set.
7. a kind of aerogens quick test pipe according to claim 6, it is characterized in that: the mouth of pipe place of outer tube is located in described capping (3) by hinged mode.
8. a kind of aerogens quick test pipe according to claim 7 is characterized in that: described outer tube (1), inner tube (2) are lucite pipe.
9. a kind of aerogens quick test pipe according to claim 1, it is characterized in that: described lucifuge pipe, lucifuge plug are plastics tubing.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210155176.5A CN103421684B (en) | 2012-05-18 | 2012-05-18 | A kind of aerogens quick test pipe |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201210155176.5A CN103421684B (en) | 2012-05-18 | 2012-05-18 | A kind of aerogens quick test pipe |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103421684A true CN103421684A (en) | 2013-12-04 |
| CN103421684B CN103421684B (en) | 2016-04-20 |
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Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201210155176.5A Active CN103421684B (en) | 2012-05-18 | 2012-05-18 | A kind of aerogens quick test pipe |
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| Country | Link |
|---|---|
| CN (1) | CN103421684B (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520539A (en) * | 2016-12-05 | 2017-03-22 | 菏泽学院 | Multi-function instrument used for microbe acid and gas producing experiment and sulfur-containing amino acid detection experiment |
| CN109679835A (en) * | 2019-01-31 | 2019-04-26 | 济南市疾病预防控制中心 | A kind of instant culture apparatus for most probable number method detection flora |
Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2322918Y (en) * | 1997-12-04 | 1999-06-09 | 蒋钱毛 | Bottle cover containing instant particles for preparing beverage |
| CN2611899Y (en) * | 2003-04-07 | 2004-04-14 | 河北农业大学 | Quick test tube for coliform group bacteria |
| US20040166556A1 (en) * | 2003-02-21 | 2004-08-26 | Cullimore Dennis Roy | Analytic device for the detection and quantification of fermentative cultured activities |
| CN202576419U (en) * | 2012-05-18 | 2012-12-05 | 杭州创新生物检控技术有限公司 | Aerogens quick-test tube |
-
2012
- 2012-05-18 CN CN201210155176.5A patent/CN103421684B/en active Active
Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN2322918Y (en) * | 1997-12-04 | 1999-06-09 | 蒋钱毛 | Bottle cover containing instant particles for preparing beverage |
| US20040166556A1 (en) * | 2003-02-21 | 2004-08-26 | Cullimore Dennis Roy | Analytic device for the detection and quantification of fermentative cultured activities |
| CN2611899Y (en) * | 2003-04-07 | 2004-04-14 | 河北农业大学 | Quick test tube for coliform group bacteria |
| CN202576419U (en) * | 2012-05-18 | 2012-12-05 | 杭州创新生物检控技术有限公司 | Aerogens quick-test tube |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106520539A (en) * | 2016-12-05 | 2017-03-22 | 菏泽学院 | Multi-function instrument used for microbe acid and gas producing experiment and sulfur-containing amino acid detection experiment |
| CN109679835A (en) * | 2019-01-31 | 2019-04-26 | 济南市疾病预防控制中心 | A kind of instant culture apparatus for most probable number method detection flora |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103421684B (en) | 2016-04-20 |
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