CN103344757A - Horizontal test paper for foot-and-mouth disease antibodies and preparation method thereof - Google Patents
Horizontal test paper for foot-and-mouth disease antibodies and preparation method thereof Download PDFInfo
- Publication number
- CN103344757A CN103344757A CN2013103177155A CN201310317715A CN103344757A CN 103344757 A CN103344757 A CN 103344757A CN 2013103177155 A CN2013103177155 A CN 2013103177155A CN 201310317715 A CN201310317715 A CN 201310317715A CN 103344757 A CN103344757 A CN 103344757A
- Authority
- CN
- China
- Prior art keywords
- foot
- mouth disease
- test paper
- detection
- test
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012360 testing method Methods 0.000 title claims abstract description 66
- 241000710198 Foot-and-mouth disease virus Species 0.000 title claims abstract description 61
- 208000007212 Foot-and-Mouth Disease Diseases 0.000 title claims abstract description 42
- 238000002360 preparation method Methods 0.000 title claims description 8
- 239000000020 Nitrocellulose Substances 0.000 claims abstract description 19
- 238000010521 absorption reaction Methods 0.000 claims abstract description 19
- 239000012528 membrane Substances 0.000 claims abstract description 19
- 229920001220 nitrocellulos Polymers 0.000 claims abstract description 19
- 239000012530 fluid Substances 0.000 claims abstract description 7
- 239000010931 gold Substances 0.000 claims abstract description 7
- 229910052737 gold Inorganic materials 0.000 claims abstract description 7
- 239000000835 fiber Substances 0.000 claims abstract description 4
- 238000001514 detection method Methods 0.000 claims description 76
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 claims description 21
- 239000007788 liquid Substances 0.000 claims description 16
- 238000002372 labelling Methods 0.000 claims description 9
- 239000013049 sediment Substances 0.000 claims description 9
- 210000002966 serum Anatomy 0.000 claims description 9
- 239000007921 spray Substances 0.000 claims description 9
- 241001465754 Metazoa Species 0.000 claims description 7
- 241000283973 Oryctolagus cuniculus Species 0.000 claims description 6
- PXIPVTKHYLBLMZ-UHFFFAOYSA-N Sodium azide Chemical compound [Na+].[N-]=[N+]=[N-] PXIPVTKHYLBLMZ-UHFFFAOYSA-N 0.000 claims description 6
- 239000000084 colloidal system Substances 0.000 claims description 6
- 238000001035 drying Methods 0.000 claims description 6
- 239000000463 material Substances 0.000 claims description 6
- 238000012545 processing Methods 0.000 claims description 6
- 239000000758 substrate Substances 0.000 claims description 6
- 239000006228 supernatant Substances 0.000 claims description 6
- 238000012546 transfer Methods 0.000 claims description 6
- 230000003612 virological effect Effects 0.000 claims description 6
- QKNYBSVHEMOAJP-UHFFFAOYSA-N 2-amino-2-(hydroxymethyl)propane-1,3-diol;hydron;chloride Chemical compound Cl.OCC(N)(CO)CO QKNYBSVHEMOAJP-UHFFFAOYSA-N 0.000 claims description 3
- 239000002250 absorbent Substances 0.000 claims description 3
- 230000002745 absorbent Effects 0.000 claims description 3
- 239000006096 absorbing agent Substances 0.000 claims description 3
- 239000000427 antigen Substances 0.000 claims description 3
- 102000036639 antigens Human genes 0.000 claims description 3
- 108091007433 antigens Proteins 0.000 claims description 3
- 244000309466 calf Species 0.000 claims description 3
- 229920002678 cellulose Polymers 0.000 claims description 3
- 239000001913 cellulose Substances 0.000 claims description 3
- 238000013016 damping Methods 0.000 claims description 3
- 238000000354 decomposition reaction Methods 0.000 claims description 3
- 239000003365 glass fiber Substances 0.000 claims description 3
- 239000008363 phosphate buffer Substances 0.000 claims description 3
- 238000003259 recombinant expression Methods 0.000 claims description 3
- 238000007789 sealing Methods 0.000 claims description 3
- 238000003756 stirring Methods 0.000 claims description 3
- 238000011144 upstream manufacturing Methods 0.000 claims description 3
- 230000004807 localization Effects 0.000 abstract 1
- 239000000243 solution Substances 0.000 description 14
- 238000005516 engineering process Methods 0.000 description 3
- 238000000034 method Methods 0.000 description 2
- 238000002965 ELISA Methods 0.000 description 1
- 241001494479 Pecora Species 0.000 description 1
- 241001493546 Suina Species 0.000 description 1
- 230000001154 acute effect Effects 0.000 description 1
- 230000004520 agglutination Effects 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 238000013461 design Methods 0.000 description 1
- 238000011161 development Methods 0.000 description 1
- 238000010586 diagram Methods 0.000 description 1
- 238000009792 diffusion process Methods 0.000 description 1
- 238000010790 dilution Methods 0.000 description 1
- 239000012895 dilution Substances 0.000 description 1
- 230000000694 effects Effects 0.000 description 1
- 210000000003 hoof Anatomy 0.000 description 1
- 230000036039 immunity Effects 0.000 description 1
- 238000002156 mixing Methods 0.000 description 1
- 230000002265 prevention Effects 0.000 description 1
- 239000011435 rock Substances 0.000 description 1
Images
Landscapes
- Investigating Or Analysing Biological Materials (AREA)
Abstract
The invention discloses a piece of horizontal test paper for foot-and-mouth disease antibodies, which comprises an oscillator, a test box and horizontal test paper for foot-and-mouth disease antibodies, wherein the horizontal test paper for foot-and-mouth disease antibodies is arranged in the test box, the side wall of the bottom of the test box is provided with a test paper inlet, and the shape of the test paper inlet fits with that of the horizontal test paper for foot-and-mouth disease antibodies, so that the horizontal test paper for foot-and-mouth disease antibodies is inserted in the test box from the test paper inlet; the horizontal test paper for foot-and-mouth disease antibodies is provided with a test area; the top of the oscillator is provided with a test fluid inlet, the bottom of the oscillator is provided with a test fluid outlet, and the test fluid outlet is provided with an openable-closeable valve; the test fluid outlet of the oscillator is communicated with an opening at the top of the test box, and the position of the opening at the top of the test box fits with the position of the test area of the horizontal test paper for foot-and-mouth disease antibodies; the test area of the horizontal test paper for foot-and-mouth disease antibodies comprises an absorption pad arranged on a baseplate, and the absorption pad is made of porous fibers; one end of the absorption pad is sequentially provided with an immune-gold localization layer, a nitrocellulose membrane and an absorption layer.
Description
?
Technical field
The present invention relates to field of medical article technology, especially relate to a kind of foot-and-mouth disease antibody level detection test paper and preparation method.
?
Background technology
Aftosa (Foot-and-Mouth, FMD) be by foot and mouth disease virus cause a kind of acute, hot, the height contagiousness animal epidemic, it mainly infects liking artiodactyls such as pig, ox, sheep, in case take place to be difficult to control, can cause significant impact to social economy.
In view of tremendous economic loss and social influence that aftosa can cause, national governments all pay much attention to the study on prevention of aftosa.Now the antibody detection method of Cai Yonging mainly contains CFT, VNT, agglutination test, immunity diffusion and ELISA etc., but these methods all need detect in the laboratory, and sense cycle is long, and row detection fast and accurately can't the counterpart hoof sprints.
?
Summary of the invention
Detect the problem that need finish at current aftosa in the laboratory, embodiment of the invention proposition is a kind of can on-the-spotly to detect to improve foot-and-mouth disease antibody level detection test paper and the preparation method who detects real-time to the aftosa antibody horizontal.
In order to realize this purpose, the invention provides a kind of foot-and-mouth disease antibody level detection test paper, comprise oscillator, detect box and be arranged at the interior foot-and-mouth disease antibody level detection test paper of described detection box; The detection zone of described foot-and-mouth disease antibody level detection test paper comprises the absorption pad that is arranged on the substrate, and described absorption pad is made by multiporous fiber; End at described absorption pad is provided with immune-gold labeled layer, nitrocellulose membrane, absorption layer successively; Described detection box bottom sidewall is provided with the test paper entrance, and the suitable so that described foot-and-mouth disease antibody level detection test paper of the shape of described test paper entrance and described foot-and-mouth disease antibody level detection test paper inserts in the described detection box from described test paper entrance; Described foot-and-mouth disease antibody level detection test paper is provided with detection zone; Described oscillator top is provided with detects the liquid input port, and the bottom is provided with detects the liquid outlet, but and the outlet of described detection liquid be provided with the valve of folding; The outlet of detection liquid and the opening conducting that detects the box top of described oscillator, the position of the position of the opening at described detection box top and the detection zone of described foot-and-mouth disease antibody level detection test paper is suitable.
Wherein, described nitrocellulose membrane is provided with the nature controlling line that forms with the rabbit of resisting O-type foot and mouth disease virus or cavy IgG antibody, the upstream that is in nature controlling line on the nitrocellulose membrane is coated with the detection line that O type foot and mouth disease virus forms, the O type foot and mouth disease virus of the colloid gold label of the detection line of nitrocellulose membrane and immune-gold labeled layer absorption is natural viral, or dna recombinant expression foot and mouth disease virus, or the decomposition antigen of foot and mouth disease virus.
?
Simultaneously, the embodiment of the invention has also proposed the preparation method of aforementioned any one foot-and-mouth disease antibody level detection test paper, comprising:
The colloidal gold solution of step 1,15～50 nanometers
The invention has the beneficial effects as follows: the present invention proposes a kind of many foot-and-mouth disease antibody level detection test papers and preparation method, can detect the aftosa antibody horizontal fast.Sample to be detected can be put into oscillator in use, rock this oscillator with abundant mixing after closing oscillator behind the adding dilution, the valve of opening the oscillator bottom then is instilled into mixed liquid on the detection zone of test paper accurately by this detection box.This structure can be used easily in the open air, improves the efficient that detects.
?
Description of drawings
By the description of a preferred embodiment of the present invention being carried out below in conjunction with accompanying drawing, it is clearer that technical scheme of the present invention and technique effect thereof will become, and easy to understand more.Wherein:
Fig. 1 is the sectional structure synoptic diagram of the foot-and-mouth disease antibody level detection test paper of the embodiment of the invention.
?
Embodiment
Below with reference to appended accompanying drawing a preferred embodiment of the present invention is described.
The invention provides a kind of foot-and-mouth disease antibody level detection test paper, its structure as shown in Figure 1, comprise oscillator 1, detect box 2 and be arranged at foot-and-mouth disease antibody level detection test paper 3 in the described detection box 2, described detection box bottom 2 sidewalls are provided with the test paper entrance, and the suitable so that described foot-and-mouth disease antibody level detection test paper 3 of the shape of described test paper entrance and described foot-and-mouth disease antibody level detection test paper 3 inserts in the described detection box 2 from described test paper entrance; Described foot-and-mouth disease antibody level detection test paper is provided with detection zone; Described oscillator top is provided with detects the liquid input port, and the bottom is provided with detects the liquid outlet, but and the outlet of described detection liquid be provided with the valve 11 of folding; The outlet of detection liquid and the opening conducting that detects the box top of described oscillator, the position of the position of the opening at described detection box 2 tops and the detection zone of described foot-and-mouth disease antibody level detection test paper 3 is suitable; The detection zone of described foot-and-mouth disease antibody level detection test paper comprises the absorption pad that is arranged on the substrate, and described absorption pad is made by multiporous fiber; End at described absorption pad is provided with immune-gold labeled layer, nitrocellulose membrane, absorption layer successively.
Wherein, described nitrocellulose membrane is provided with the nature controlling line that forms with the rabbit of resisting O-type foot and mouth disease virus or cavy IgG antibody, the upstream that is in nature controlling line on the nitrocellulose membrane is coated with the detection line that O type foot and mouth disease virus forms, the O type foot and mouth disease virus of the colloid gold label of the detection line of nitrocellulose membrane and immune-gold labeled layer absorption is natural viral, or dna recombinant expression foot and mouth disease virus, or the decomposition antigen of foot and mouth disease virus.
?
Simultaneously, the embodiment of the invention has also proposed the preparation method of aforementioned any one foot-and-mouth disease antibody level detection test paper, comprising:
The colloidal gold solution of step 1,15～50 nanometers
For the person of ordinary skill in the field, along with the development of technology, the present invention's design can realize by different way.Embodiments of the present invention are not limited in embodiment described above, and can change within the scope of the claims.
Claims (3)
1. a foot-and-mouth disease antibody level detection test paper is characterized in that, comprises oscillator, detects box and is arranged at the interior foot-and-mouth disease antibody level detection test paper of described detection box; The detection zone of described foot-and-mouth disease antibody level detection test paper comprises the absorption pad that is arranged on the substrate, and described absorption pad is made by multiporous fiber; End at described absorption pad is provided with immune-gold labeled layer, nitrocellulose membrane, absorption layer successively; Described detection box bottom sidewall is provided with the test paper entrance, and the suitable so that described foot-and-mouth disease antibody level detection test paper of the shape of described test paper entrance and described foot-and-mouth disease antibody level detection test paper inserts in the described detection box from described test paper entrance; Described foot-and-mouth disease antibody level detection test paper is provided with detection zone; Described oscillator top is provided with detects the liquid input port, and the bottom is provided with detects the liquid outlet, but and the outlet of described detection liquid be provided with the valve of folding; The outlet of detection liquid and the opening conducting that detects the box top of described oscillator, the position of the position of the opening at described detection box top and the detection zone of described foot-and-mouth disease antibody level detection test paper is suitable.
2. foot-and-mouth disease antibody level detection test paper according to claim 1, it is characterized in that, described nitrocellulose membrane is provided with the nature controlling line that forms with the rabbit of resisting O-type foot and mouth disease virus or cavy IgG antibody, the upstream that is in nature controlling line on the nitrocellulose membrane is coated with the detection line that O type foot and mouth disease virus forms, the O type foot and mouth disease virus of the colloid gold label of the detection line of nitrocellulose membrane and immune-gold labeled layer absorption is natural viral, or dna recombinant expression foot and mouth disease virus, or the decomposition antigen of foot and mouth disease virus.
3. the preparation method of a foot-and-mouth disease antibody level detection test paper as claimed in claim 1 or 2 comprises:
The colloidal gold solution of step 1,15～50 nanometers
Step 2, pH value with colloidal gold solution transfers to 8.0～8.4 earlier, add 0.1～0.4mg foot and mouth disease virus by every 100ml colloidal gold solution therein again, after stirring, in solution, add animal blood serum albumen by 0.2～1g/100ml again, 4 ℃ left standstill 2～4 hours, again with the centrifugal removal sediment of above-mentioned colloidal gold solution, get supernatant, supernatant is got sediment through centrifugal treating again, the gained sediment is dissolved in the viral solution that obtains colloid gold label in the damping fluid of 0.02M pH7.4 Tris-Hcl of the animal blood serum albumen that contains weight ratio 0.2～0.6% and 0.01～0.06% Sodium azide by 4～10ml/100ml, till colloidal gold solution immersed glass fibre to liquid and begin to ooze out, form golden labeling antibody layer, golden labeling antibody layer is carried out drying handle;
Step 3, at nitrocellulose filter stage casing spray detection line, getting the anti-or mouse resisting O-type foot and mouth disease IgG of rabbit again, to transfer concentration be 1.5～2.5mg/ml, in the cellulose membrane stage casing, apart from detection line 0.5～1cm place, spray nature controlling line with Membrane jetter, spray film amount arranges by 10～15ul/1cm, the dry processing then, the phosphate buffer that contains calf serum with 0.01m PH7.0 carries out the drying processing again and obtains nitrocellulose filter 37 ℃ of sealings 30 minutes down after the PBS rinsing of 0.01ml pH7.0 again;
Step 3, golden labeling antibody layer, detection segment and absorbent pad material and absorber portion are fixed on adopt on the substrate that impermeable material makes successively.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103177155A CN103344757A (en) | 2013-07-25 | 2013-07-25 | Horizontal test paper for foot-and-mouth disease antibodies and preparation method thereof |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013103177155A CN103344757A (en) | 2013-07-25 | 2013-07-25 | Horizontal test paper for foot-and-mouth disease antibodies and preparation method thereof |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103344757A true CN103344757A (en) | 2013-10-09 |
Family
ID=49279571
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013103177155A Pending CN103344757A (en) | 2013-07-25 | 2013-07-25 | Horizontal test paper for foot-and-mouth disease antibodies and preparation method thereof |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103344757A (en) |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645682A (en) * | 2016-11-30 | 2017-05-10 | 中国农业科学院兰州兽医研究所 | Colloidal gold test paper for detecting Asia I-type foot-and-mouth disease and preparation method of colloidal gold test paper |
| CN106770228A (en) * | 2016-11-30 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | One kind is used for organophosphorus pesticide detection kit |
Citations (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2262986A (en) * | 1992-01-03 | 1993-07-07 | Pall Corp | Particle agglutination assay |
| JP2001235408A (en) * | 2000-02-23 | 2001-08-31 | Nippon Koden Corp | Sample dilution apparatus and method |
| JP2001281242A (en) * | 2000-03-30 | 2001-10-10 | Matsushita Electric Ind Co Ltd | Biological sample measuring device |
| CN1509409A (en) * | 2001-05-18 | 2004-06-30 | ����ʵ�鼼����˾ | In-line test device and methods of use |
| CN1877331A (en) * | 2006-07-07 | 2006-12-13 | 中国农业科学院兰州兽医研究所 | Foot-and-mouth disease virus detecting test paper tape and its preparation method and using method |
| CN1882836A (en) * | 2003-11-14 | 2006-12-20 | 香港澳维有限公司 | Device for rapid detection and storage of samples and method of use thereof |
| CN1900115A (en) * | 2006-07-11 | 2007-01-24 | 中国农业科学院兰州兽医研究所 | Method for preparing monoclonal antibody resisting O-type foot and mouth disease virus and antibody and use |
| CN2929903Y (en) * | 2006-07-27 | 2007-08-01 | 万国勇 | Electric horn base of motor vehicle |
| CN200989903Y (en) * | 2006-10-27 | 2007-12-12 | 万华普曼生物工程有限公司 | Poisonous drug five-in-one union detecting plate |
| CN101236204A (en) * | 2007-11-29 | 2008-08-06 | 中国农业科学院兰州兽医研究所 | O-type foot-and-mouth disease antibody level detection test paper and preparation method |
| CN101241138A (en) * | 2007-11-29 | 2008-08-13 | 中国农业科学院兰州兽医研究所 | Asia 1 type foot-and-mouth disease antibody level detection test paper and preparation method |
| CN101256639A (en) * | 2008-04-10 | 2008-09-03 | 山东省寄生虫病防治研究所 | Minitype aquatic insect counting and racking machine |
| CN101464457A (en) * | 2008-07-23 | 2009-06-24 | 吕欣龙 | Detecting instrument for liquid sample |
| CN102053149A (en) * | 2010-11-12 | 2011-05-11 | 万华普曼生物工程有限公司 | Body fluid detection device with detection and storage functions |
| CN102151510A (en) * | 2011-02-14 | 2011-08-17 | 四川迈克生物科技股份有限公司 | Mixing device |
| CN202230080U (en) * | 2011-09-19 | 2012-05-23 | 崔凤玲 | Test tube for test paper |
| CN102645361A (en) * | 2012-04-24 | 2012-08-22 | 柳州职业技术学院 | Automatic treatment device for animal feed AFB1 content detection sample |
-
2013
- 2013-07-25 CN CN2013103177155A patent/CN103344757A/en active Pending
Patent Citations (17)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GB2262986A (en) * | 1992-01-03 | 1993-07-07 | Pall Corp | Particle agglutination assay |
| JP2001235408A (en) * | 2000-02-23 | 2001-08-31 | Nippon Koden Corp | Sample dilution apparatus and method |
| JP2001281242A (en) * | 2000-03-30 | 2001-10-10 | Matsushita Electric Ind Co Ltd | Biological sample measuring device |
| CN1509409A (en) * | 2001-05-18 | 2004-06-30 | ����ʵ�鼼����˾ | In-line test device and methods of use |
| CN1882836A (en) * | 2003-11-14 | 2006-12-20 | 香港澳维有限公司 | Device for rapid detection and storage of samples and method of use thereof |
| CN1877331A (en) * | 2006-07-07 | 2006-12-13 | 中国农业科学院兰州兽医研究所 | Foot-and-mouth disease virus detecting test paper tape and its preparation method and using method |
| CN1900115A (en) * | 2006-07-11 | 2007-01-24 | 中国农业科学院兰州兽医研究所 | Method for preparing monoclonal antibody resisting O-type foot and mouth disease virus and antibody and use |
| CN2929903Y (en) * | 2006-07-27 | 2007-08-01 | 万国勇 | Electric horn base of motor vehicle |
| CN200989903Y (en) * | 2006-10-27 | 2007-12-12 | 万华普曼生物工程有限公司 | Poisonous drug five-in-one union detecting plate |
| CN101236204A (en) * | 2007-11-29 | 2008-08-06 | 中国农业科学院兰州兽医研究所 | O-type foot-and-mouth disease antibody level detection test paper and preparation method |
| CN101241138A (en) * | 2007-11-29 | 2008-08-13 | 中国农业科学院兰州兽医研究所 | Asia 1 type foot-and-mouth disease antibody level detection test paper and preparation method |
| CN101256639A (en) * | 2008-04-10 | 2008-09-03 | 山东省寄生虫病防治研究所 | Minitype aquatic insect counting and racking machine |
| CN101464457A (en) * | 2008-07-23 | 2009-06-24 | 吕欣龙 | Detecting instrument for liquid sample |
| CN102053149A (en) * | 2010-11-12 | 2011-05-11 | 万华普曼生物工程有限公司 | Body fluid detection device with detection and storage functions |
| CN102151510A (en) * | 2011-02-14 | 2011-08-17 | 四川迈克生物科技股份有限公司 | Mixing device |
| CN202230080U (en) * | 2011-09-19 | 2012-05-23 | 崔凤玲 | Test tube for test paper |
| CN102645361A (en) * | 2012-04-24 | 2012-08-22 | 柳州职业技术学院 | Automatic treatment device for animal feed AFB1 content detection sample |
Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN106645682A (en) * | 2016-11-30 | 2017-05-10 | 中国农业科学院兰州兽医研究所 | Colloidal gold test paper for detecting Asia I-type foot-and-mouth disease and preparation method of colloidal gold test paper |
| CN106770228A (en) * | 2016-11-30 | 2017-05-31 | 百奥森（江苏）食品安全科技有限公司 | One kind is used for organophosphorus pesticide detection kit |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| Deleers et al. | Covid-19 and blood groups: ABO antibody levels may also matter | |
| CN103472229B (en) | A kind of carcinomebryonic antigen magnetic microparticle chemiluminescence immune assay detection kit | |
| CN104181298B (en) | A kind of human parainfluenza virus's IgM antibody test strip and preparation method thereof | |
| CN106248974A (en) | A kind of hypersensitivity immunity chromatography detection test paper | |
| CN107942061A (en) | A kind of test card, preparation and its detection method for detecting transmissible gastro-enteritis virus antibody | |
| CN101339192A (en) | Test strips for one-step detection of porcine viral diarrhea disease pathogens | |
| CN102747040A (en) | A kind of anti-influenza virus nucleoprotein monoclonal antibody and its preparation and application | |
| CN105606808A (en) | Group A/B streptococcus colloidal gold immunochromatograohic assay detection device and detection method thereof | |
| CN109154605A (en) | Immunochromatography detection method | |
| CN101285839B (en) | Quick detection method for newcastle disease virus and its immune colloidal gold test card | |
| CN110389219A (en) | A kind of enrichment detecting method of Epithelial and stromal mixed type and PD-L1 positive circulating tumor cell | |
| CN105319359B (en) | Streptococcus pneumoniae quantum dot immunochromatographic assay detection card and preparing method and application thereof | |
| CN104142401A (en) | Bladder tumor-associated antigen detection kit | |
| CN101968488A (en) | Colloidal gold fast diagnostic test strip of goose paramyxovirus disease | |
| CN203838160U (en) | ELISA detection kit for gB and gE antibodies of porcine pseudorabies virus | |
| CN103091490B (en) | A kind of A type aftosa 146S antigen quantify ELISA detection method and kit thereof and application | |
| CN103344757A (en) | Horizontal test paper for foot-and-mouth disease antibodies and preparation method thereof | |
| CN101936997A (en) | Human anti-rabies virus IgG antibody ELISA test kit | |
| CN109161530A (en) | A kind of separation method of the circulating tumor cell based on phenyl boric acid | |
| CN103954772B (en) | Three gold mark detection test paper bars of a kind of CSFV, pig breeding and respiratory disorder syndrome virus, Pseudorabies virus antibody | |
| CN103777022A (en) | Method for detecting content of virus antigen in encephalitis B vaccine | |
| CN112142833B (en) | A kind of furosemide artificial antigen, antibody and its application in detecting furosemide | |
| CN103389374B (en) | Micro whole blood application of sample method detects mycoplasma pneumoniae colloidal gold kit | |
| CN103389379A (en) | Test strip and method for detecting tylosin and tilmicosin | |
| CN103713136B (en) | Immunity five detects basic agent |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C12 | Rejection of a patent application after its publication | ||
| RJ01 | Rejection of invention patent application after publication |
Application publication date: 20131009 |