CN103333845B - Pseudomonas chlororaphis and fermenting cultivation method thereof - Google Patents
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Abstract
The invention relates to a pseudomonas chlororaphis and a fermenting cultivation method thereof. The preservation number of the pseudomonas chlororaphis in China general microbiological culture collection center (CGMCC) is CGMCC No. 6773, and the fermenting cultivation method comprises the following steps: 1) inoculating activated pseudomonas chlororaphis nlsy001 into special fermentation medium for the pseudomonas chlororaphis nlsy001 for 18-28 hours at the temperature of 26-28 DEG C at the pH of 6.5-7.2 so as to cultivate seed solution; and 2) inoculating the prepared seed solution obtained in the step 1) to the special fermentation medium for the pseudomonas chlororaphis nlsy001 at the temperature of 26-28 DEG C at the pH of 6.5-7.2 so as to aerobically culture, wherein the pseudomonas chlororaphis nlsy001 CGMCC No. 6773 is microbial inoculum of active ingredient. Compared with the prior art, the pseudomonas chlororaphis nlsy001 CGMCC No. 6773 has short fermentation period, is simple in preparation process of the microbial inoculum, and has probability of commercial process.
Description
Technical field
The present invention relates to a strain biocontrol strain, especially relate to a kind of Pseudomonas chlororaphis and fermentation culture method thereof.
Background technology
The bacterium that some are useful is there is in plant rhizosphere, they can play preventive and therapeutic effect by direct or indirect mode Promoting plant growth to by the microbial Plant diseases of cause of disease, these can Promoting plant growth, controlling disease the microorganism increasing crop yield is referred to as plant growth-promoting rhizobacteria (plant growthpromoting rhizobacteria, PGPR).This type of rhizospheric microorganism abundant species is various, select suitable rhizosphere growth-promoting endophytic bacteria to process (as seed soaking or spraying) plant, rhizosphere environment, Promoting plant growth can be improved, suppress or alleviate Plant diseases, reduce chemical fertilizer and agricultural chemicals input, alleviate the effects such as environmental pollution.Bacterium provided by the invention is separated from plant root to obtain, and belongs to rhizosphere growth-promoting endophytic bacteria.
Summary of the invention
Object of the present invention is exactly to provide a kind of Pseudomonas chlororaphis and the fermentation culture method thereof with controlling disease effect.
Object of the present invention can be achieved through the following technical solutions:
A kind of Pseudomonas chlororaphis, it is characterized in that, this Pseudomonas chlororaphis, is CGMCC No.6773 at the deposit number at China Committee for Culture Collection of Microorganisms's common micro-organisms center.
The morphological specificity of described Pseudomonas chlororaphis is: thalline is shaft-like, 0.3 μm ~ 0.8 μm × 1.0 μm ~ 1.1 μm, Gram-negative, without gemma, single polar flagella, can move, 1.2mm bacterium colony can be formed after KMB substratum cultivates 24h, bacterium colony can produce orange pigment, circular, rat, smooth, comparatively thickness, easily provokes, neat in edge.
The physiological and biochemical property of described Pseudomonas chlororaphis is: can produce fluorochrome, can be hydrolyzed arginine dihydrolase, lipase, oxydase, catalase, Citrate trianion, gelatin, not hydrolyzed starch, also not produce H
2s, can not utilize poly-β-hydroxy butyrate to make carbon source.The suitableeest culture temperature is 28 DEG C ~ 30 DEG C, and the most suitable growth pH value is 7.0 ~ 7.5, can produce orange non-fluorescence pigment, can utilize lecithinase, can be Polylevulosan sucrose inversion, not produce pyocyanin, also do not carry out denitrification.The NaCl concentration of the most suitable growth is 0 ~ 6%, when NaCl concentration is greater than 7%, grows suppressed.
A fermentation culture method for Pseudomonas chlororaphis, is characterized in that, the method comprises the following steps:
1) activated Pseudomonas chlororaphis nlsy001 is inoculated in this bacterium fermenting substratum, 26-28 DEG C, cultivate 18-28 hour under pH6.5-7.2, obtain seed liquor;
2) by step 1) seed liquor prepared is inoculated in this bacterium fermenting substratum, at 26-28 DEG C, aerated culture under pH6.5-7.2;
Described step 1) and step 2) in special fermentative medium formula be: containing Semen Maydis powder 10-15 gram in every 1000 ml waters, soybean cake powder 30-40 gram, K
2hPO
40.1-0.2 gram, KH
2pO
40.15-0.25 gram, MgSO
40.4-0.5 gram, initial pH6.5-7.5.
Step 1) in the method that Pseudomonas chlororaphis nlsy001 bacterial strain activates be: by this Pseudomonas chlororaphis nlsy001 inoculation in KB substratum, at 26-28 DEG C, cultivate 18-28 hour; The formula of described KB is: containing containing peptone 20 grams, glycerine 15 milliliters, K in every 1000 ml waters
2hPO
40.392 gram and MgSO
40.732 gram, pH is 6.5-7.5.
Described Pseudomonas chlororaphis nlsy001 fermenting culture medium prescription is: containing Semen Maydis powder 10 grams in every 1000 ml waters, soybean cake powder 35 grams, K
2hPO
40.15 gram, KH
2pO
40.2 gram, MgSO
40.4-0.5 gram, initial pH6.5-7.5.
Step 1) in inoculative proportion be 2-5%, culture condition be 26 DEG C, under pH6.8,220rpm concussion cultivate; Step 2) in inoculative proportion be 6-8%, air flow is with fermented liquid and per minute air flow volume basis for 1: 0.2-0.4, and the tank pressure cultivating culture tank used is 1.2-1.8F/cm
2, culture tank is with whipping appts, and stirring velocity is 180-240rpm, and incubation time is 24-48 hour.
Step 2) fermentation culture conditions for 26 DEG C, carry out under pH6.8, incubation time is 36-48 hour.
An application for Pseudomonas chlororaphis, is characterized in that, the microbial inoculum being activeconstituents with Pseudomonas chlororaphis nlsy001CGMCCNo6773.
After stopping composition aseptic to the fermented liquid of Pseudomonas chlororaphis nlsy001CGMCC No6773 and drying is mixed by weight 5-10: 1, press filtration, then it is air-dry to shade, and obtains Pseudomonas chlororaphis nlsy001CGMCCNo6773 microbial inoculum; Described stopping composition is the peat composed of rotten mosses or diatomite, and fineness is 150-250 order.
The described microbial inoculum obtained is that to be adjusted to final water content be less than 35% for the 150-250 object peat composed of rotten mosses, diatomite or kaolin.
In actual production, according to the requirement of microbial inoculum water content, Pseudomonas chlororaphis nlsy001 microbial inoculum is mixed with stopping composition, after press filtration, can add a certain amount of auxiliary agent again, be preferably diatomite, its fineness is 150-200 order, be preferably 200 orders, the addition of auxiliary agent can be determined according to the practical situation of bacterium amount and water content, under the prerequisite not affecting product effect, makes the water content of Pseudomonas chlororaphis nlsy001 microbial inoculum reach 30-35%.
Apply Pseudomonas chlororaphis nlsy001 of the present invention or can be with the method for its microbial inoculum controlling plant diseases being activeconstituents: when plant-growth is to the 3-4 leaf phase, being taken out by seedling, is 10 by concentration
7-10
9the bacterium immersion root 20-40 minute of cfu/mL, maybe the seedling of taking-up is watered filling root with the microbial inoculum that biocontrol microorganisms nlsy001 is activeconstituents, the ratio of weight and number that microbial inoculum mixes with water is 1: 400-600, then through transplanting, cultivating, obtains the plant of disease resistance.
In above-mentioned control of plant disease method, the bacterium liquid preferred concentration of biocontrol microorganisms nlsy001 is 10
8cfu/mL, the leaching root time is preferably 30 minutes; The ratio of weight and number that the microbial inoculum being activeconstituents with biocontrol microorganisms nlsy001 mixes with water is 1: 500.
Compared with prior art, the microbial inoculum that the invention provides a strain biocontrol microorganisms nlsy001 and be activeconstituents with it, greenhouse pot culture and field test prove that this bacterial strain has prophylaxis effect, to various plants soil-borne disease, as capsicum epidemic disease, watermelon blight, rice sheath blight disease and sclerotinia rot of colza have significant prevention effect.The main mechanism of controlling plant diseases is its good plant rhizosphere colonization ability and can produces the antibiotic agents suppressing growth of pathogenic bacteria.
Biocontrol microorganisms nlsy001 fermentation period is short, and its microbial inoculum preparation technology is simple, has the possibility of suitability for industrialized production.The present invention has broad application prospects in the control of plant soil-borne diseases.
Embodiment
In following implementation example, method therefor is ordinary method if no special instructions, and all percentage concentrations are mass percentage concentration, and the solvent in all substratum is water.
The formula of KB is: containing peptone 20 grams, glycerine 15 milliliters, K in every 1000mL water
2hPO40.392 gram and MgSO
40.732 gram, pH is 6.5-7.5.
Biocontrol microorganisms nlsy001 fermenting culture medium prescription is: containing Semen Maydis powder 10 grams in every 1000mL water, soybean cake powder 35 grams, K
2hPO
40.15 gram, KH
2pO
40.2 gram, MgSO
40.4-0.5 gram, initial pH6.5-7.5.
Embodiment 1:
The culture presevation of Pseudomonas chlororaphis nlsy001 and fermentation culture thereof
1, culture presevation
Biocontrol microorganisms nlsy001 is located away from Anhui Province's wheat foundation soil, and be preserved in China Committee for Culture Collection of Microorganisms's common micro-organisms center on November 2nd, 2012, deposit number is CGMCCNo.6773.
2, the fermentation of biocontrol microorganisms nlsy001
The fermentation process of biocontrol microorganisms nlsy001 comprises the following steps:
1) activation of bacterial classification
By biocontrol microorganisms nlsy001 inoculation in KB substratum, cultivate 20 hours at 26 DEG C.
2) seed tank culture
By step 1) activated biocontrol microorganisms nlsy001 is inoculated in this bacterium fermenting substratum by 4% inoculative proportion, 26 DEG C, shake cultivation 30 hours under pH6.8,220rpm, obtain seed liquor.
3) fermentor cultivation
By step 1) be inoculated in the fermenting substratum of this bacterium through the seed liquor of preparation by 5% inoculative proportion, 26 DEG C, pH6.8, stirring velocity 220rpm, air flow 1: 0.2-0.4 (fermented liquid and per minute air flow volume ratio), tank pressure be 1.2-1.8F/cm
2lower cultivation obtains biocontrol microorganisms nlsy001 bacterium liquid for 45 hours.
Embodiment 2:
The preparation of biocontrol microorganisms nlsy001 microbial inoculum
The preparation method of biocontrol microorganisms nlsy001 microbial inoculum is the biocontrol microorganisms nlsy001 bacterium liquid that embodiment 1 fermentation obtained with dry, aseptic, fineness to be 200 object diatomite be by ratio of weight and the number of copies 9: 1 mix after, press filtration, adding 200 appropriate object diatomite again makes bacterium liquid mix with it, cover shady air-dry, obtain biocontrol microorganisms nlsy001 microbial inoculum.Qualified through inspection finished product, water content is 34%, and viable bacteria content is greater than 8,000,000,000/gram.
Embodiment 3:
Detect biocontrol microorganisms nlsy001 to the test of control of plant disease effect
For Pepper Phytophthora Blight, detect biocontrol microorganisms nlsy001 to the prevention effect of Plant diseases, concrete grammar is: when the green pepper seedling that stand-by sterilized soil is cultivated grows to the 3-4 leaf phase, and being taken out by seedling, is 10 by concentration
8the biocontrol microorganisms nlsy001 bacterium immersion root of cfu/mL 30 minutes, maybe the seedling of taking-up is watered filling root with the microbial inoculum that biocontrol microorganisms nlsy001 is activeconstituents, the ratio of weight and number that microbial inoculum mixes with water is 1: 500, with unprocessed and fill with the plant of root with aqua sterilisa and compare, again seedling is moved in the seedling alms bowl of 8cm bore, slow seedling after 4 days inoculum density be 10
7the Pepper Phytophthora Blight spore suspension 50mL/ alms bowl of cfu/mL, regularly according to disease scale method investigation disease index after inoculation pathogenic bacteria, result reaches more than 85% through biocontrol microorganisms nlsy001 bacterium liquid or with the prevention effect of the plant of its microbial inoculum process being activeconstituents.Potted plant and the field resistance test-results of the watermelon blight undertaken by same procedure, rice sheath blight disease and sclerotinia rot of colza shows bacterial strain of the present invention to the prevention effect of above-mentioned disease also higher than 65%, and above-mentioned experimental result all shows that biocontrol microorganisms nlsy001 of the present invention can significantly improve the disease resistance of plant.
Embodiment 4
A kind of Pseudomonas chlororaphis, this Pseudomonas chlororaphis is CGMCC No6773 at the deposit number at China Committee for Culture Collection of Microorganisms's common micro-organisms center.The morphological specificity of described Pseudomonas chlororaphis is: thalline is shaft-like, 0.3 μm ~ 0.8 μm × 1.0 μm ~ 1.1 μm, Gram-negative, without gemma, single polar flagella, can move, 1.2mm bacterium colony can be formed after KMB substratum cultivates 24h, bacterium colony can produce orange pigment, circular, rat, smooth, comparatively thickness, easily provokes, neat in edge.The physiological and biochemical property of described Pseudomonas chlororaphis is: can produce fluorochrome, can be hydrolyzed arginine dihydrolase, lipase, oxydase, catalase, Citrate trianion, gelatin, not hydrolyzed starch, also not produce H
2s, can not utilize poly-β-hydroxy butyrate to make carbon source.The suitableeest culture temperature is 28 DEG C ~ 30 DEG C, and the most suitable growth pH value is 7.0 ~ 7.5, can produce orange non-fluorescence pigment, can utilize lecithinase, can be Polylevulosan sucrose inversion, not produce pyocyanin, also do not carry out denitrification.The NaCl concentration of the most suitable growth is 0 ~ 6%, when NaCl concentration is greater than 7%, grows suppressed.
The fermentation culture method of above-mentioned Pseudomonas chlororaphis, the method comprises the following steps:
1) be: by this Pseudomonas chlororaphis nlsy001 inoculation in KB substratum cultivate 28 hours at 26 DEG C the method that Pseudomonas chlororaphis nlsy001 bacterial strain activates; The formula of described KB is: containing containing peptone 20 grams, glycerine 15 milliliters, K in every 1000 ml waters
2hPO
40.392 gram and MgSO
40.732 gram, pH is 6.5-7.5.
2) be inoculated in this bacterium fermenting substratum by activated Pseudomonas chlororaphis nlsy001, inoculative proportion is 2%, 26 DEG C, under pH6.5,220rpm concussion cultivate lower cultivation 28 hours, obtain seed liquor;
3) by step 1) seed liquor prepared is inoculated in this bacterium fermenting substratum, and inoculative proportion is 6%, at 26 DEG C, aerated culture under pH6.5; Air flow is with fermented liquid and per minute air flow volume basis for 1: 0.2, and the tank pressure cultivating culture tank used is 1.2F/cm
2, culture tank is with whipping appts, and stirring velocity is 180rpm, and incubation time is 48 hours.
Described step 1) and step 2) in special fermentative medium formula be: containing Semen Maydis powder 10 grams in every 1000 ml waters, soybean cake powder 30 grams, K
2hPO
40.1 gram, KH
2pO
40.15 gram, MgSO
40.4 gram, initial pH6.5.
The application of above-mentioned Pseudomonas chlororaphis, with the microbial inoculum that Pseudomonas chlororaphis nlsy001CGMCC No6773 is activeconstituents, be specially: after stopping composition aseptic to the fermented liquid of Pseudomonas chlororaphis nlsy001CGMCC No6773 and drying is mixed by weight 5: 1, press filtration, shade again air-dry, obtain Pseudomonas chlororaphis nlsy001CGMCC No6773 microbial inoculum; Described stopping composition is the peat composed of rotten mosses or diatomite, and fineness is 150 orders.
In actual production, according to the requirement of microbial inoculum water content, Pseudomonas chlororaphis nlsy001 microbial inoculum is mixed with stopping composition, after press filtration, can add a certain amount of auxiliary agent again, be preferably diatomite, its fineness is 150-200 order, be preferably 200 orders, the addition of auxiliary agent can be determined according to the practical situation of bacterium amount and water content, under the prerequisite not affecting product effect, makes the water content of Pseudomonas chlororaphis nlsy001 microbial inoculum reach 30-35%.
Apply Pseudomonas chlororaphis nlsy001 of the present invention or can be with the method for its microbial inoculum controlling plant diseases being activeconstituents: when plant-growth is to the 3-4 leaf phase, being taken out by seedling, is 10 by concentration
7-10
9the bacterium immersion root 20-40 minute of cfu/mL, maybe the seedling of taking-up is watered filling root with the microbial inoculum that biocontrol microorganisms nlsy001 is activeconstituents, the ratio of weight and number that microbial inoculum mixes with water is 1: 400-600, then through transplanting, cultivating, obtains the plant of disease resistance.
In above-mentioned control of plant disease method, the bacterium liquid preferred concentration of biocontrol microorganisms nlsy001 is 10
8cfu/mL, the leaching root time is preferably 30 minutes; The ratio of weight and number that the microbial inoculum being activeconstituents with biocontrol microorganisms nlsy001 mixes with water is 1: 500.
Embodiment 5
A kind of Pseudomonas chlororaphis, this Pseudomonas chlororaphis is CGMCC No6773 at the deposit number at China Committee for Culture Collection of Microorganisms's common micro-organisms center.The morphological specificity of described Pseudomonas chlororaphis is: thalline is shaft-like, 0.3 μm ~ 0.8 μm × 1.0 μm ~ 1.1 μm, Gram-negative, without gemma, single polar flagella, can move, 1.2mm bacterium colony can be formed after KMB substratum cultivates 24h, bacterium colony can produce orange pigment, circular, rat, smooth, comparatively thickness, easily provokes, neat in edge.The physiological and biochemical property of described Pseudomonas chlororaphis is: can produce fluorochrome, can be hydrolyzed arginine dihydrolase, lipase, oxydase, catalase, Citrate trianion, gelatin, not hydrolyzed starch, also not produce H
2s, can not utilize poly-β-hydroxy butyrate to make carbon source.The suitableeest culture temperature is 28 DEG C ~ 30 DEG C, and the most suitable growth pH value is 7.0 ~ 7.5, can produce orange non-fluorescence pigment, can utilize lecithinase, can be Polylevulosan sucrose inversion, not produce pyocyanin, also do not carry out denitrification.The NaCl concentration of the most suitable growth is 0 ~ 6%, when NaCl concentration is greater than 7%, grows suppressed.
The fermentation culture method of above-mentioned Pseudomonas chlororaphis, the method comprises the following steps:
1) be: by this Pseudomonas chlororaphis nlsy001 inoculation in KB substratum cultivate 28 hours at 28 DEG C the method that Pseudomonas chlororaphis nlsy001 bacterial strain activates; The formula of described KB is: containing containing peptone 20 grams, glycerine 15 milliliters, K in every 1000 ml waters
2hPO
40.392 gram and MgSO
40.732 gram, pH is 6.5-7.5.
2) be inoculated in this bacterium fermenting substratum by activated Pseudomonas chlororaphis nlsy001, inoculative proportion is 5%, 28 DEG C, under pH7.2,220rpm concussion cultivate lower cultivation 18 hours, obtain seed liquor;
3) by step 1) seed liquor prepared is inoculated in this bacterium fermenting substratum, and inoculative proportion is 8%, at 28 DEG C, aerated culture under pH7.2; Air flow is with fermented liquid and per minute air flow volume basis for 1: 0.4, and the tank pressure cultivating culture tank used is 1.8F/cm
2, culture tank is with whipping appts, and stirring velocity is 240rpm, and incubation time is 24 hours.
Described step 1) and step 2) in special fermentative medium formula be: containing Semen Maydis powder 15 grams in every 1000 ml waters, soybean cake powder 40 grams, K
2hPO
40.2 gram, KH
2pO
40.25 gram, MgSO
40.5 gram, initial pH7.5.
The application of above-mentioned Pseudomonas chlororaphis, with the microbial inoculum that Pseudomonas chlororaphis nlsy001CGMCC No6773 is activeconstituents, be specially: after stopping composition aseptic to the fermented liquid of Pseudomonas chlororaphis nlsy001CGMCC No6773 and drying is mixed by weight 10: 1, press filtration, shade again air-dry, obtain Pseudomonas chlororaphis nlsy001CGMCC No6773 microbial inoculum; Described stopping composition is the peat composed of rotten mosses or diatomite, and fineness is 250 orders.
In actual production, according to the requirement of microbial inoculum water content, Pseudomonas chlororaphis nlsy001 microbial inoculum is mixed with stopping composition, after press filtration, can add a certain amount of auxiliary agent again, be preferably diatomite, its fineness is 150-200 order, be preferably 200 orders, the addition of auxiliary agent can be determined according to the practical situation of bacterium amount and water content, under the prerequisite not affecting product effect, makes the water content of Pseudomonas chlororaphis nlsy001 microbial inoculum reach 30-35%.
Apply Pseudomonas chlororaphis nlsy001 of the present invention or can be with the method for its microbial inoculum controlling plant diseases being activeconstituents: when plant-growth is to the 3-4 leaf phase, being taken out by seedling, is 10 by concentration
7-10
9the bacterium immersion root 20-40 minute of cfu/mL, maybe the seedling of taking-up is watered filling root with the microbial inoculum that biocontrol microorganisms nlsy001 is activeconstituents, the ratio of weight and number that microbial inoculum mixes with water is 1: 400-600, then through transplanting, cultivating, obtains the plant of disease resistance.
In above-mentioned control of plant disease method, the bacterium liquid preferred concentration of biocontrol microorganisms nlsy001 is 10
8cfu/mL, the leaching root time is preferably 30 minutes; The ratio of weight and number that the microbial inoculum being activeconstituents with biocontrol microorganisms nlsy001 mixes with water is 1: 500.
Claims (5)
1. a fermentation culture method for Pseudomonas chlororaphis, is characterized in that, the method comprises the following steps:
1) activated Pseudomonas chlororaphis is inoculated in this bacterium fermenting substratum, 26-28 DEG C, cultivate 18-28 hour under pH6.5-7.2, obtain seed liquor;
2) by step 1) seed liquor prepared is inoculated in this bacterium fermenting substratum, at 26-28 DEG C, aerated culture under pH6.5-7.2;
Described step 1) and step 2) in special fermentative medium formula be: containing Semen Maydis powder 10-15 gram in every 1000 ml waters, soybean cake powder 30-40 gram, K
2hPO
40.1-0.2 gram, KH
2pO
40.15-0.25 gram, MgSO
40.4-0.5 gram, initial pH6.5-7.5.
2. the fermentation culture method of Pseudomonas chlororaphis according to claim 1, it is characterized in that, step 1) in the method that Pseudomonas chlororaphis bacterial strain activates be: by this Pseudomonas chlororaphis inoculation in KB substratum, at 26-28 DEG C, cultivate 18-28 hour; The formula of described KB is: containing peptone 20 grams, glycerine 15 milliliters, K in every 1000 ml waters
2hPO
40.392 gram and MgSO
40.732 gram, pH is 6.5-7.5.
3. the fermentation culture method of Pseudomonas chlororaphis according to claim 1, is characterized in that, described Pseudomonas chlororaphis fermenting culture medium prescription is: containing Semen Maydis powder 10 grams in every 1000 ml waters, soybean cake powder 35 grams, K
2hPO
40.15 gram, KH
2pO
40.2 gram, MgSO
40.4-0.5 gram, initial pH6.5-7.5.
4. the fermentation culture method of Pseudomonas chlororaphis according to claim 1, is characterized in that, step 1) in inoculative proportion be 2-5%, culture condition be 26 DEG C, under pH6.8,220rpm concussion cultivate; Step 2) in inoculative proportion be 6-8%, air flow is with fermented liquid and per minute air flow volume basis for 1:0.2-0.4, and the tank pressure cultivating culture tank used is 1.2-1.8F/cm
2, culture tank is with whipping appts, and stirring velocity is 180-240rpm, and incubation time is 24-48 hour.
5. the fermentation culture method of Pseudomonas chlororaphis according to claim 1, is characterized in that, step 2) fermentation culture conditions for 26 DEG C, carry out under pH6.8, incubation time is 36-48 hour.
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| CN103602621B (en) * | 2013-12-02 | 2015-04-15 | 江苏省农业科学院 | Pseudomonas choloeaphtis and application |
| CN103787712B (en) * | 2014-03-11 | 2016-05-18 | 南通禾宝生物科技有限公司 | The preparation method of fertilizer |
| CN105925501B (en) * | 2016-05-09 | 2019-06-11 | 中国水稻研究所 | A kind of pseudomonas and its application |
| CN107099474B (en) * | 2017-05-12 | 2019-12-31 | 山东省烟台市农业科学研究院 | A kind of Pseudomonas aeruginosa with broad-spectrum antibacterial activity and its application |
| CN110144301B (en) * | 2018-02-10 | 2022-04-01 | 河南农业大学 | Pseudomonas chlororaphis and culture method and application thereof |
| CN108410779B (en) * | 2018-05-14 | 2019-11-05 | 中国科学院生态环境研究中心 | A kind of microbial inoculum comprising Meng Shi pseudomonad |
| CN111944728B (en) * | 2020-08-26 | 2022-02-22 | 河南大学 | Pseudomonas chlororaphis and application thereof |
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