CN103333800B - Dynamic-static positive-negative pressure loading experiment system and method for in-vitro cells - Google Patents
Dynamic-static positive-negative pressure loading experiment system and method for in-vitro cells Download PDFInfo
- Publication number
- CN103333800B CN103333800B CN201310254749.4A CN201310254749A CN103333800B CN 103333800 B CN103333800 B CN 103333800B CN 201310254749 A CN201310254749 A CN 201310254749A CN 103333800 B CN103333800 B CN 103333800B
- Authority
- CN
- China
- Prior art keywords
- pressure
- incubator
- temperature
- gas
- valve
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Expired - Fee Related
Links
Landscapes
- Apparatus Associated With Microorganisms And Enzymes (AREA)
Abstract
本发明涉及一种体外细胞动静态正-负压加载实验系统及方法，该系统包括气源储气瓶，减压阀，电磁气阀，过滤器，分流器，高压缓冲容器，常压缓冲容器，高压容器，低压容器，真空泵，压缩泵，压力培养箱，数显温度变送器，数显压力表带变送器，数据采集系统，驱动控制系统，加热器，水浴槽，其中气源储气瓶通过减压阀、电磁气阀、过滤器、压力容器和压力培养箱相连；压力培养箱通过压力容器和真空泵、压缩泵相连；压力培养箱通过压力传感器及温度传感器和数据采集系统及驱动控制系统相连。本发明能够在体外模拟多种细胞的体内力学环境尤其是颞颌关节腔的压力环境，为体外研究在体细胞的生物力学响应提供研究平台。
The invention relates to an in vitro cell dynamic and static positive-negative pressure loading experiment system and method. The system includes a gas source gas storage bottle, a pressure reducing valve, an electromagnetic gas valve, a filter, a shunt, a high-pressure buffer container, and a normal-pressure buffer container , high-pressure containers, low-pressure containers, vacuum pumps, compression pumps, pressure incubators, digital display temperature transmitters, digital display pressure gauges with transmitters, data acquisition systems, drive control systems, heaters, water baths, of which gas source storage The gas cylinder is connected to the pressure incubator through a pressure reducing valve, electromagnetic valve, filter, pressure vessel; the pressure incubator is connected to a vacuum pump and a compression pump through a pressure vessel; the pressure incubator is connected to a pressure sensor, a temperature sensor, a data acquisition system and a driver connected to the control system. The invention can simulate the internal mechanical environment of various cells in vitro, especially the pressure environment of the temporomandibular joint cavity, and provides a research platform for studying the biomechanical response of somatic cells in vitro.
Description
Technical field
The present invention relates to a kind of cell in vitro sound state n-negative pressure loading experiment system and method thereof, belong to cell biomechanics and organizational engineering field.
Background technology
Cell biomechanics is an interactional science of research cell and mechanical factor, is the important integral part of organizational engineering.Under the organism effect in various mechanical forces constantly, the multi-form effects such as Er body tissue and cell are also often under pressure, shearing force, distraction force, force of compression, under different mechanical functions, form a mechanical system, at the various activities of cell, play an important role in as propagation, differentiation, adhesion, migration, apoptosis.But it is single effect or joint effect that numerous mechanics factors is difficult to distinguish in vivo.Therefore for clear and definite mechanical stimulation, the effect in the activities such as the growth of cell, differentiation has directive significance to the biomechanics effect of, studying single mechanics factor.At present, the development of cell in vitro Experiments of Machanics deceleration loading device has become the main method of research cell biomechanics.Pressure is the common mechanics form of cell in vivo, the most common in joint.Temporomandibular joint is bearing from malleation and is changing with negative pressure and along with the different mode of motion of lower jaw in moving process, and therefore, the pressure environment that can simulate temporomandibular joint is significant with regeneration at cartilage remodeling for research mandibular condylar chondrocytes.
Development along with tissue engineering technique, stem cell is with its vigorous propagation and differentiation potential, for tissue transplantation with the better method that provides is provided, it is not enough that but its maximum defect is generated cartilage cell epimatrix mechanical property, and stem cell can be divided into the more cartilaginous tissue of horn of plenty of matrix components under suitable pressure-acting.Therefore, design the n-negative pressure loading experiment of a kind of multi-functional cell in vitro sound state system and study the especially biomechanical response under mandibular condylar chondrocytes mechanical function of various kinds of cell for us, further the signal transduction mechanism of the form of announcement cell cell under different pressure-acting conditions, propagation, differentiation, apoptosis, sets up in vitro study model to organizational engineering.
At present, the pressure-loaded experimental installation of foreign study cell is to take that the cell pressure-loaded system of Yousefian development is representative.And at home, this area has been developed dissimilar cell pressure-loaded experimental installation, still, these experimental installations are the limitation of various degrees also.Specifically comprise:, although existing experimental installation can load static pressure and dynamic pressure, does not also simulate dynamic negative-pressure and dynamic n-negative pressure (1), be difficult to meet the needs to temporomandibular joint research.(2) existing experimental installation can't regulate and control the pressure-loaded environment of cell, is difficult to meet to the long pressure-loaded experiment of cell.(3) pattern of pressure of existing experimental installation is more single, and the narrow range of pressure-loaded is difficult to meet the research of different pressures environment cell in body.
Summary of the invention
The object of the invention is a kind of cell in vitro sound state n-negative pressure loading experiment system of development and a kind of method of cell pressure-loaded is provided, be characterized in vitro cell being specified to the pressure that can load various ways under culture condition, comprise (static malleation, static negative pressure, dynamic malleation, dynamic negative-pressure and dynamic n-negative pressure), and can the size of on-load pressure, frequency, action time and pressure-acting pattern be controlled and be regulated.Experimental system can be under the prerequisite that does not affect pressure the gas in discharge section experimental system open air inlet electromagnetic valve tonifying Qi by the program of setting regularly, change the gas in whole experimental system according to the time of setting, keep the CO of culture environment
2dividing potential drop and O
2dividing potential drop is constant, thereby keeps pH value and the HCO of cell culture fluid
3 -stablizing of concentration.Under different pressure-acting patterns, by two kinds of type of heating, keep pressure to cultivate the constant of the temperature inside the box, by these methods, keep the three-dimensional environment of the cell cultures in pressure incubator.That this pilot system has advantages of is simple to operate, stable performance, precise control, can simulate in vitro the mechanical environment in various kinds of cell body, sets up in vitro study at the platform of somatic biomechanical response.
The present invention's cell pressure-loaded experimental system used is characterized in can simulating at somatic mechanical environment, comprises static malleation, static negative pressure, dynamic malleation, dynamic negative-pressure and dynamic n-negative pressure.The principle of work of system is: the culture dish of culturing cell or culture plate are put into incubator, open bomb switch, gas passes through reducing valve from source of the gas bomb, electromagnetic gas valve and strainer enter four-way splitter, enter again normal pressure buffer container, the gas of normal pressure buffer container pumps into high-pressure buffer container and low pressure vessel by compression pump, by the control of high-voltage electromagnetic air valve and low voltage electromagnetic air valve, pass through five-way splitter respectively again and enter into pressure incubator, gas in incubator enters normal pressure buffer container by five-way splitter and normal pressure electromagnetic gas valve again, form the closed cycle of a gas.Gas in incubator also can be discharged by exhaust electromagnetic gas valve, to complete ventilation.Now, according to pressure-acting pattern, pressure size, frequency, the action time set, control mainboard controls compression pump by driving control system and vacuum pump is worked, for corresponding static malleation, static negative pressure, dynamic malleation, dynamic negative-pressure and dynamic n-negative pressure are provided in incubator, pressure and temperature in pressure incubator and each pressure buffer container all can complete collection by data collecting system by each pressure transmitter and temperature sensor simultaneously, controlling mainboard can indicating system each several part pressure and temperature parameter, and regulates according to setting program.In experimentation, situation about can cultivate by the transparent toughened glass observation of cell of pressure incubator hatch door, arrives and sets after the working hour, controls mainboard and finishes pressure-loaded according to the parameter of setting.Open exhaust solenoid valve, the gas of emptying incubator, opens incubator hatch door.
The object of invention is realized by following technical measures:
This device comprises: containing 5%CO
2source of the gas, pressure incubator, pressure controlling system, temperature adjusting system, data collecting system and the driving control system of mixing air.Source of the gas bomb is connected with pressure incubator by the first reducing valve, electromagnetic gas valve, strainer, four-way splitter, normal pressure buffer container, high-pressure buffer container, low pressure vessel, vacuum pump, compression pump; The other end of pressure incubator is connected with band sourdine exhaust electromagnetic gas valve; Band sourdine exhaust electromagnetic gas valve is connected with driving control system and is presented on display interface; Pressure incubator one end and digital display manometer band transmitter, the first temperature digital display transmitter, the other end is connected with data collecting system; High-pressure buffer container, low pressure vessel are connected with pressure indicator; Normal pressure buffer container one end is connected with pressure unit, and the other end is connected with data collecting system; High-pressure buffer container one end is connected with pressure incubator by electromagnetic gas valve, and the other end is connected to driving control system by splitter and compression pump; Low pressure vessel one end is connected with pressure incubator by electromagnetic gas valve, and the other end is connected to driving control system by splitter and vacuum pump; Normal pressure buffer container, high-pressure buffer container, low pressure vessel are inserted in water bath; Water bath one end connects well heater, and the other end connects water level switch, temperature digital display transmitter is connected with data collecting system; Pressure controlling system, temperature adjusting system, data collecting system and driving control system are connected and are presented on control panel with control mainboard respectively.
Pressure is cultivated in casing and is established three layers of culture dish support, support is hollow type, can hold orifice plate and culture dish for experiment, and make the interior gas of incubator easily with the gaseous interchange in culture dish, the inlet mouth of pressure incubator is arranged on the side of incubator, and baffle plate is set prevents because erratic flowing appears in gas in air inlet and the incubator causing of giving vent to anger frequently, thereby avoid the pressure in incubator inhomogeneous.
Incubator hatch door is designed to 4 screws and fixes, and hatch door mouth seals with sealing-ring, and there is circular viewing window in hatch door central authorities, is high-strength armoured-glass, can realize the cell omnidistance transparent observing of pressurizeing.
The n-negative pressure loading method of cell in vitro sound state of the present invention comprises the following steps:
(1) subject cell is put into pressure incubator together with culture dish, close incubator hatch door, seal-off pressure culture chamber;
(2) open bomb air valve, gas by reducing valve, strainer, normal pressure buffer container enters into high-pressure buffer container and low pressure vessel by compression pump and vacuum pump respectively, by solenoid control, enter into incubator again, pressure in incubator enters normal pressure buffer container by magnetic valve, forms the closed cycle of a gas and is full of 95% air and 5%CO
2mixed gas, water bath and fixedly heating unit make the temperature in incubator reach 36 ± 2 ℃;
(3) set static pressure pattern, action time, compression pump/vacuum pump pattern or dynamic pressure pattern, action time, compression pump and vacuum pump pattern, frequency, by driving control system driving power system and magnetic valve, regulate incubator internal pressure;
(4) pressure incubator internal pressure is communicated to computer main board by pressure transmitter, by the pressure in the program regulation and control incubator setting;
(5) for the loading regime that requires static pressure, control mainboard by drive compression pump/vacuum pump, make the pressure in incubator reach set(ting)value, according to the program of setting, regulate the variation of pressure simultaneously, control the constant of incubator internal pressure.
(6) for requiring dynamic pressure loading regime, control mainboard by drive compression pump and vacuum pump, and make compression pump and vacuum pump coordination operation, regulate the variation of pressure tank internal gas pressure, make incubator internal pressure according to frequency and the size variation set, and monitor by pressure sensing transmitter, reach requirement of experiment.
Source of the gas is 5%CO
2with the mixed gas of 95% air, utilize medical air filter cleaning gas, guarantee gas gnotobasis; Pressure incubator selects pollution-free, avirulent medical acid-resistant stainless steel materials processing to form, and pressure incubator inwall designs for circular arc, without dead angle, is convenient to cleaning and disinfection.
Type of heating is Water Tank with Temp.-controlled and assisted heating device combined heat, and when being set as static pressure, because gas does not flow in incubator, therefore controlling mainboard, to control fixedly heating unit be incubator direct heating.When setting pressure is dynamic pressure, because gas flows rapidly between pressure incubator, normal pressure buffer container, high-pressure buffer container and low pressure vessel, have heat loss, control mainboard can control Water Tank with Temp.-controlled and fixedly heating unit jointly heat, and keep homo(io)thermism in incubator.
Data collecting system comprises pressure sensing transmitter, temperature sensing transmitter, and is connected with control mainboard.
The program that control mainboard writes is the core of regulation and control pressure change, and its function is as follows:
Drive compression pump and vacuum pump produce air pressure, make the certain pressure of generation in high-pressure buffer container and low pressure vessel, high-pressure buffer container, low pressure vessel circulate mutually with incubator, and to producing the dynamic or static pressure of certain frequency, size in pressure incubator, monitor the running status of compression pump and vacuum pump simultaneously, by driving control system is regulated, make pressure culture systems stable.
Solenoid control function: the switch of controlling magnetic valve by controlling mainboard regulates air inlet and exhaust, thus by control magnetic valve switching time adjustments of gas flow fine adjustment incubator internal pressure.
Data processing and Presentation Function: according to the frequency of setting, pressure, working hour, moving/static pressure pattern, control mainboard drive compression pump and vacuum pump work, by pressure buffer container, the pressure in incubator is regulated, produce a certain size static pressure or certain frequency and big or small dynamic pressure.Meanwhile, by data collecting system, the temperature in pressure incubator, pressure are carried out to data gathering, and be presented on display interface, with wave, dynamically demonstrate the process of pressure change simultaneously, can monitor in real time the temperature in incubator, pressure.
The present invention has following advantage:
(1) type of heating of the present invention is Water Tank with Temp.-controlled and fixing heating unit combined heat, and under static pressure pattern, gas remains static in incubator, fixedly heating unit work keep incubator homo(io)thermism at 37 ℃.Under dynamic pressure pattern, gas is rapid flow in normal pressure buffer container, high-pressure buffer appearance, high pressure vessel and low pressure vessel and pressure incubator, Water Tank with Temp.-controlled is the pressurized vessel heating being immersed in water-bath, guarantee that the gas temperature in whole system keeps constant temperature, and the scattering and disappearing of gas heat in make-up system pipeline, by this type of heating, guarantee the stable of whole cell culture environment constant temperature, guarantee cell bio-activity, reach good cell constant temperature culture environment.Simultaneously, under dynamic pressure pattern, gas is heat production after Fast Compression in pressure incubator, can cause the temperature in cell culture incubator sharply to change, and gas can keep cultivating the constant of the temperature inside the box after exchanging between pressure incubator and pressure buffer container.
(2) in the present invention, what pressure-driven system adopted is compression pump and vacuum pump, malleation and negative pressure in a big way can be provided, pressure buffer container and pressure incubator all adopt high voltage bearing stainless steel to make simultaneously, therefore can carry out the cyto-mechanics that pressure range is wide, pressure pattern is many (static negative pressure, static malleation, dynamic negative-pressure, dynamic malleation and dynamic n-negative pressure) to cell loads, different pressure environments is provided, can be applicable to various kinds of cell mechanics study.
(3) in the present invention, because pressure incubator is closed environment, under the long pressurized state of cell, can change the proportion of composing of gas, in the gas of mainboard Controlling System in the program timing discharge section experimental system according to setting, according to the program of setting, open air inlet electromagnetic valve tonifying Qi, and change the gas in whole experimental system and do not affect the stability of system in the interior time of setting, keep the CO of culture environment
2dividing potential drop and O
2dividing potential drop is constant, thereby keeps pH value and the HCO of cell culture fluid
3 -the good living environment of stable assurance cell of concentration.
(4) in the present invention, control panel is simple to operation, can show in real time pressure, temperature, frequency, action time, the pressure of buffer container, variation track and the pressure waveform curve of pressure incubator internal pressure of pressure incubator, and monitor in real time to the parameters to incubator by data collecting system.
(5) in the present invention, sound state pressure-controlling is intelligent: under static schema, and compression pump or vacuum pump work, when pressure incubator inside reaches after setting pressure, compression pump or vacuum pump quit work.When incubator air pressure inside changes, data collecting system detects and collects after pressure data, controls mainboard order and opens air inlet electromagnetic valve or exhaust solenoid valve, regulates incubator internal gas, keeps the constant pressure of incubator inside.
Accompanying drawing explanation
Fig. 1 is the n-negative pressure loading experimental apparatus of cell in vitro sound state
In figure: 1, source of the gas bomb, 2, the first reducing valve, 3, electromagnetic gas valve, 4, strainer, 5, four-way splitter, 6, normal pressure buffer container, 7, vacuum pump, 8, compression pump, 9, safety valve, 10, high pressure vessel, 11, the first pointer tensimeter, 12, the second reducing valve, 13, low pressure vessel, 14, high-pressure buffer container, 15, the second pointer tensimeter, 16, the first pressure unit, 17, the 3rd pointer tensimeter, 18, low voltage electromagnetic air valve, 19, the 4th pointer tensimeter, 20, the second pressure unit, 21, high-voltage electromagnetic air valve, 22, band sourdine exhaust electromagnetic gas valve, 23, normal pressure electromagnetic gas valve, 24, five-way splitter, 25, pressure incubator, 26, the first temperature digital display transmitter, 27, digital display manometer band transmitter, 28, data collecting system, 29, display interface, 30, operating panel, 31, control mainboard, 32, switch power supply, 33, driving control system, 34, well heater, 35, the second temperature digital display transmitter, 36, water bath, 37, water level switch.
Fig. 2 is pressure incubator schematic diagram
In figure: 25, pressure incubator, 26, temperature digital display transmitter, 27, digital display manometer band transmitter, 38, air inlet air outlet, 39, Tissue Culture Dish, 40, toughened glass viewing window, 41, culture dish support, 42, cell cultures box bracket.
Fig. 3 is the n-negative pressure loading experiment of cell in vitro sound state system display interface
Fig. 4 is the programflow chart of the n-negative pressure loading experiment of cell in vitro sound state system
Embodiment
Below in conjunction with accompanying drawing, principle of the present invention and feature are described, example is just for explaining and further illustrate the present invention, and non-limiting scope of the present invention.Various nonessential improvement and adjustment that the researchist who is skilled in technique in this area makes technical scheme of the present invention, all should include protection scope of the present invention in.
With reference to the gas in accompanying drawing 1 source of the gas bomb 1 of the present invention, by the first reducing valve 2, electromagnetic gas valve 3 and strainer 4, enter four-way splitter 5.Other three ends of four-way splitter 5 are connected with normal pressure buffer container 6, vacuum pump 7, compression pump 8 respectively.Compression pump 8 is connected with high pressure vessel 10, safety valve 9, the first pointer tensimeter 11 are connected with high pressure vessel 10, high pressure vessel 10 is connected with high-pressure buffer container 14 by the second reducing valve 12, the pressure that the second pointer tensimeter 15 shows in high-pressure buffer container 14, the gas in high-pressure buffer container 14 is connected with five-way splitter 24 by high-voltage electromagnetic air valve 21.One end of low pressure vessel 13 is connected with vacuum pump 7, and the other end of low pressure vessel 13 is connected with one end of low voltage electromagnetic air valve 18, the other end of low voltage electromagnetic air valve 18 be connected with five-way splitter 24.The first pressure unit 16, the 3rd pointer tensimeter 17 are sampled and show gaseous tension in low pressure vessel 13 respectively.One end of normal pressure buffer container 6 is connected with four-way splitter 5, and the other end is connected with normal pressure electromagnetic gas valve 23; The 4th pointer tensimeter 19, the second pressure unit 20 show and sample gaseous tension in normal pressure buffer container 6 respectively.Band sourdine exhaust electromagnetic gas valve 22 is connected with five-way splitter 24; Pressure incubator 25 is connected with five-way splitter 24, the first temperature digital display transmitter 26, and digital display manometer band transmitter 27 shows and samples gaseous tension and temperature in normal pressure buffer container 6 respectively.Water in 34 pairs of water baths 36 of well heater heats, and the water temperature in the second 35 pairs of temperature digital display transmitters water bath 36 gathers, and the water level in 37 pairs of water baths 36 of water level switch gathers.Data collecting system 28 is connected with each data sampling sensor of system, and is connected with control mainboard 31, and switch power supply 32, driving control system 33, display interface 29 and operating panel 30 are connected with control mainboard 31 respectively.
Pressure is cultivated in casing 25 and is established three layers of culture dish support 41, support is hollow type, can hold Tissue Culture Dish 39, and make gas easily with the gaseous interchange in culture dish, reach setting air pressure, the inlet mouth 38 of pressure incubator is arranged on the side of incubator simultaneously, and baffle plate is set prevents from because of air inlet frequently and give vent to anger causing the gas in incubator to occur erratic flowing, thereby avoid the pressure in incubator inhomogeneous.
Incubator hatch door is designed to 4 screws and fixes, and hatch door mouth seals with sealing-ring, and there is circular tempering glass window 40 in hatch door central authorities, can realize the cell omnidistance transparent observing of pressurizeing.
The n-negative pressure loading method of cell in vitro sound state of the present invention comprises the following steps:
(1) open source of the gas bomb air valve, source of the gas gas-holder and pressure are cultivated in casing and be full of 95% air and 5%CO
2mixed gas;
(2) culture dish is put into pressure incubator, closed and airtight described pressure incubator;
(3) set pressure pattern, pressure size, frequency and the action time needing, control mainboard and control magnetic valve by driving circuit, regulate and control described pressure and cultivate loading pressure;
(4) described pressure is cultivated loading pressure and is conveyed to control mainboard by pressure transmitter, by program, regulates described pressure cultivate the variation of loading pressure and reach setting pressure;
(5) described pressure is cultivated the temperature inside the box and is sent to control mainboard by temperature sensor, by program, regulates the variation of temperature and the variation of holding temperature;
(6) for the loading regime of static pressure, by set experiment parameter on control panel, comprise pressure size, positive/negative die pressing type, action time, compression pump/vacuum pump pattern, described control mainboard is by driving control system drive compression pump/vacuum pump, regulate the pressure in described pressure incubator, temperature digital display transmitter and digital pressure transducer be monitor temperature and pressure respectively, and be sent to control mainboard by data collecting system, control mainboard and by setup parameter, regulate and guarantee that the pressure and temperature in described pressure incubator is constant;
(7) for the loading regime of dynamic pressure, by set experiment parameter on described control panel, comprise pressure size, dynamic malleation/dynamic negative-pressure/dynamic n-negative pressure pattern, frequency, action time, compression pump+vacuum pump pattern, control mainboard by driving control system drive compression pump and vacuum pump, regulate the variation of the pressure in described pressure incubator, temperature digital display transmitter and digital pressure transducer be monitoring pressure and temperature parameter respectively, and be sent to control mainboard by data collecting system, adjust the pressure and temperature in described pressure incubator, and the dynamic pressure reaching in described pressure incubator changes.
Claims (2)
1. the n-negative pressure loading experiment of a cell in vitro sound state system, is characterized in that this system comprises source of the gas bomb (1), the first reducing valve (2), electromagnetic gas valve (3), strainer (4), four-way splitter (5), normal pressure buffer container (6), vacuum pump (7), compression pump (8), safety valve (9), high pressure vessel (10), the first pointer tensimeter (11), the second reducing valve (12), low pressure vessel (13), high-pressure buffer container (14), the second pointer tensimeter (15), the first pressure unit (16), the 3rd pointer tensimeter (17), low voltage electromagnetic air valve (18), the 4th pointer tensimeter (19), the second pressure unit (20), high-voltage electromagnetic air valve (21), band sourdine exhaust electromagnetic gas valve (22), normal pressure electromagnetic gas valve (23), five-way splitter (24), pressure incubator (25), the first temperature digital display transmitter (26), digital display manometer band transmitter (27), data collecting system (28), display interface (29), operating panel (30), control mainboard (31), switch power supply (32), driving control system (33), well heater (34), the second temperature digital display transmitter (35), water bath (36), water level switch (37), gas in described source of the gas bomb (1) is by described the first reducing valve (2), electromagnetic gas valve (3) and strainer (4) enter one end (5) of described four-way splitter, other three ends of described four-way splitter (5) respectively with described normal pressure buffer container (6), vacuum pump (7), compression pump (8) is connected, described compression pump (8), safety valve (9), the first pointer tensimeter (11) is connected with described high pressure vessel (10) respectively, described high pressure vessel (10) is connected with described high-pressure buffer container (14) by described the second reducing valve (12), described the second pointer tensimeter (15) shows the pressure in described high-pressure buffer container (14), gas in described high-pressure buffer container (14) is connected with described five-way splitter (24) by described high-voltage electromagnetic air valve (21), one end of described low pressure vessel (13) is connected with described vacuum pump (7), the other end of described low pressure vessel (13) is connected with one end of described low voltage electromagnetic air valve (18), the other end of described low voltage electromagnetic air valve (18) is connected with described five-way splitter (24), described the first pressure unit (16), the 3rd pointer tensimeter (17) is sampled and shows gaseous tension in described low pressure vessel (13) respectively, one end of described normal pressure buffer container (6) is connected with described four-way splitter (5), the other end of described normal pressure buffer container (6) is connected with described normal pressure electromagnetic gas valve (23), described the 4th pointer tensimeter (19), the second pressure unit (20) show and sample gaseous tension in described normal pressure buffer container (6) respectively, and described band sourdine exhaust electromagnetic gas valve (22) is connected with described five-way splitter (24), described pressure incubator (25) is connected with described five-way splitter (24), described the first temperature digital display transmitter (26), digital display manometer band transmitter (27) shows and samples gaseous tension in described normal pressure buffer container (6) and temperature respectively, described well heater (34) heats the water in described water bath (36), described the second temperature digital display transmitter (35) gathers the water temperature in described water bath (36), described water level switch (37) gathers the water level in described water bath (36), described data collecting system (28) and the first temperature digital display transmitter (26), digital display manometer band transmitter (27), the second temperature digital display transmitter (35) is connected, and described data collecting system (28) is connected with described control mainboard (31), described switch power supply (32), driving control system (33), display interface (29) is connected with described control mainboard (31) respectively with operating panel (30), in described pressure incubator (25), establish three layers of culture dish support, support is hollow out shape, can hold orifice plate and culture dish for experiment, makes gas easily with the gaseous interchange in culture dish and reach setting air pressure, the hatch door central authorities of described pressure incubator (25) arrange window, are transparent toughened glass, can carry out visible observation to the cell whole process of pressurizeing, by be placed in high-pressure buffer container (14), low pressure vessel (13), the normal pressure buffer container (6) of water bath (36) with well heater (34) heating, gas in heating system, and by being placed on pressure incubator (25) described in the fixedly heating unit direct heating of pressure incubator (25) below, thereby by controlling mainboard (31), control and regulate the temperature in pressure incubator, described the first temperature digital display transmitter (26) is sampled and shows the temperature in incubator.
2. the performed pressure-loaded method of the n-negative pressure loading experiment of cell in vitro sound state system as claimed in claim 1, is characterized in that said method comprising the steps of:
(1) open source of the gas bomb air valve, source of the gas gas-holder and pressure are cultivated in casing and be full of 95% air and 5%CO
2mixed gas;
(2) culture dish is put into pressure incubator, closed and airtight described pressure incubator;
(3) set pressure pattern, pressure size, frequency and the action time needing, control mainboard and control magnetic valve by driving circuit, regulate and control described pressure and cultivate loading pressure;
(4) described pressure is cultivated loading pressure and is conveyed to control mainboard by pressure transmitter, by program, regulates described pressure cultivate the variation of loading pressure and reach setting pressure;
(5) described pressure is cultivated the temperature inside the box and is sent to control mainboard by temperature sensor, by program, regulates the variation of temperature and the variation of holding temperature;
(6) for the loading regime of static pressure, by set experiment parameter on control panel, comprise pressure size, positive/negative die pressing type, action time, compression pump/vacuum pump pattern, described control mainboard is by driving control system drive compression pump/vacuum pump, regulate the pressure in described pressure incubator, temperature digital display transmitter and digital display manometer band transmitter be monitor temperature and pressure respectively, and be sent to control mainboard by data collecting system, control mainboard and by setup parameter, regulate and guarantee that the pressure and temperature in described pressure incubator is constant;
(7) for the loading regime of dynamic pressure, by set experiment parameter on described control panel, comprise pressure size, dynamic malleation/dynamic negative-pressure/dynamic n-negative pressure pattern, frequency, action time, compression pump+vacuum pump pattern, control mainboard by driving control system drive compression pump and vacuum pump, regulate the variation of the pressure in described pressure incubator, temperature digital display transmitter and digital pressure transducer be monitoring pressure and temperature parameter respectively, and be sent to control mainboard by data collecting system, adjust the pressure and temperature in described pressure incubator, and the dynamic pressure reaching in described pressure incubator changes.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310254749.4A CN103333800B (en) | 2013-06-09 | 2013-06-09 | Dynamic-static positive-negative pressure loading experiment system and method for in-vitro cells |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN201310254749.4A CN103333800B (en) | 2013-06-09 | 2013-06-09 | Dynamic-static positive-negative pressure loading experiment system and method for in-vitro cells |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| CN103333800A CN103333800A (en) | 2013-10-02 |
| CN103333800B true CN103333800B (en) | 2014-10-15 |
Family
ID=49242005
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN201310254749.4A Expired - Fee Related CN103333800B (en) | 2013-06-09 | 2013-06-09 | Dynamic-static positive-negative pressure loading experiment system and method for in-vitro cells |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103333800B (en) |
Families Citing this family (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN107012088B (en) * | 2017-05-12 | 2023-11-24 | 中国人民解放军总医院 | A pressure-adjustable bacterial incubator |
| CN109581230A (en) * | 2018-11-27 | 2019-04-05 | 上海理工大学 | Soft-package battery outer surface constant pressure that compressed gas is adjustable pre-tightens test device and method |
| CN113005035A (en) * | 2021-03-26 | 2021-06-22 | 中国海洋大学 | Multichannel uniform tensile stress in-vitro cell culture device and working method |
| WO2023232099A1 (en) * | 2022-06-02 | 2023-12-07 | 北京中赢谷投资管理有限公司 | Method for obtaining vesicles |
| CN115369037B (en) * | 2022-10-24 | 2023-01-31 | 中国中医科学院望京医院(中国中医科学院骨伤科研究所) | Full circulation device, method and loading system for spinal culture solution |
| CN118846274B (en) * | 2024-09-25 | 2024-11-29 | 大连理工大学 | A device for regulating wound healing by dynamic negative pressure drainage and a verification method of an in vitro cell culture model |
Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201065407Y (en) * | 2007-06-14 | 2008-05-28 | 华中科技大学同济医学院附属同济医院 | In vitro cell open pressure program-controlled culture device |
| CN101717719A (en) * | 2009-12-08 | 2010-06-02 | 四川大学 | In-vitro cell pressure loading device and in-vitro cell pressure loading method thereof |
| CN201737949U (en) * | 2010-07-12 | 2011-02-09 | 中国人民解放军第三军医大学第一附属医院 | Cell culturing device capable of periodically converting positive pressure and negative pressure |
-
2013
- 2013-06-09 CN CN201310254749.4A patent/CN103333800B/en not_active Expired - Fee Related
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN201065407Y (en) * | 2007-06-14 | 2008-05-28 | 华中科技大学同济医学院附属同济医院 | In vitro cell open pressure program-controlled culture device |
| CN101717719A (en) * | 2009-12-08 | 2010-06-02 | 四川大学 | In-vitro cell pressure loading device and in-vitro cell pressure loading method thereof |
| CN201737949U (en) * | 2010-07-12 | 2011-02-09 | 中国人民解放军第三军医大学第一附属医院 | Cell culturing device capable of periodically converting positive pressure and negative pressure |
Non-Patent Citations (4)
| Title |
|---|
| 体外细胞压应力加载装置的研制与应用;李明黎等;《中国组织工程研究与临床康复》;20090528;第13卷(第22期);4291-4294 * |
| 基于真空泵的正负压连续控制系统;李锦云等;《机械工程学报》;20051130;第41卷(第11期);53-57 * |
| 李明黎等.体外细胞压应力加载装置的研制与应用.《中国组织工程研究与临床康复》.2009,第13卷(第22期),4291-4294. |
| 李锦云等.基于真空泵的正负压连续控制系统.《机械工程学报》.2005,第41卷(第11期),53-57. |
Also Published As
| Publication number | Publication date |
|---|---|
| CN103333800A (en) | 2013-10-02 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN103333800B (en) | Dynamic-static positive-negative pressure loading experiment system and method for in-vitro cells | |
| CN212025366U (en) | Cell tissue culture device capable of accurately controlling gas pressure and concentration | |
| CN103756898B (en) | A kind of stressed culture apparatus of cell three-dimensional applying dynamic load | |
| CN209797991U (en) | In-situ observation cell culture box | |
| WO2019120135A1 (en) | Cell culture module and cell culture system | |
| CN102329729B (en) | A Culture System for Simulation of Microgravity Effects in Suspension Cells | |
| CN104762206B (en) | Cell in-vitro culture device and culture method | |
| CN105331536A (en) | Cell cultivation device applied to microscopic observation | |
| CN112501020B (en) | Biological tissue culture system based on micro-fluidic chip and implementation operation method thereof | |
| CN113302276A (en) | Dendritic cell generation apparatus and method | |
| CN107699483A (en) | A kind of medical test microbial cultivation equipment | |
| CN105543092B (en) | Dynamic and Non-contact cyto-mechanics stimulates loading device | |
| CN101486968B (en) | Intellectualized bionic cultivation apparatus for tissue engineering tissue | |
| WO2019120136A1 (en) | Cell culture module, culture solution module, cell culture chip, and cell culture case | |
| CN203498391U (en) | Positive-negative-pressure dynamic-static loading testing device for cells in vitro | |
| CN210656959U (en) | Biological culture system with pressure stress stimulation function | |
| CN204455070U (en) | A kind of cell fluid mechanics experimental apparatus | |
| CN104031837B (en) | A joint motion simulation test system with biological cultivation function | |
| CN111334429A (en) | Modular multifunctional cell workstation | |
| CN101665766B (en) | Negative-pressure cell culture apparatus and method thereof | |
| CN109294909A (en) | A kind of cell observation incubator | |
| CN204311067U (en) | A kind of organizational project stem cell inducing cardiomyocytes is cultivated and is measured bio-reactor | |
| CN103756899B (en) | Can be used for biological cultured continuously and the microscopic imaging device of stable state high-intensity magnetic field effect study | |
| CN104388311B (en) | Organizational project stem cell inducing cardiomyocytes is cultivated and measures bioreactor | |
| CN2923715Y (en) | Automatic candidate steam cell culturing system |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C10 | Entry into substantive examination | ||
| SE01 | Entry into force of request for substantive examination | ||
| C14 | Grant of patent or utility model | ||
| GR01 | Patent grant | ||
| CF01 | Termination of patent right due to non-payment of annual fee |
Granted publication date: 20141015 |
|
| CF01 | Termination of patent right due to non-payment of annual fee |