CN103329801A - Method for rooting culture of sugarcane tissue culture seedling - Google Patents

Abstract

A method for rooting culture of sugarcane tissue cultured seedlings, comprising the steps of: inserting proliferated sterile tissue cultured seedlings into MS+sodium hypochlorite 800-1200mg/L+white sugar 30g/L medium, sealing, and placing the culture room at a temperature of 27±1 ℃, 12-16 hours of light per day to carry out rooting culture for 20-30 days to grow a complete root system. In the rooting method of the present invention, the culture medium does not contain exogenous hormones, no high-pressure sterilization is required, and the inoculation does not require the use of an ultra-clean workbench, the operation is simple and fast, the pollution rate is low, and the cost of rooting culture can be reduced by 40-60%. Rooted seedlings grow vigorously.

Description

A kind of method for sugarcane tissue culture seedling rooting culture

technical field

The invention relates to the field of plant tissue culture, in particular to a method for rooting culture of sugarcane tissue culture seedlings.

Background technique

Plant tissue culture has: 1. Save a lot of space and time; 2. The cultivation process is not affected by external environmental factors, and can be carried out all year round; 3. Repeatability and other three advantages. It is often widely promoted and applied in the fields of large-scale propagation of virus-free seedlings, mutation breeding, transgenics, production of secondary metabolites, artificial seeds, etc., and has produced huge benefits. However, tissue culture technology has strict requirements on a sterile environment, resulting in a significant increase in application costs. The complex technical links and instruments in the tissue culture process, such as aseptic operating tables, biosafety cabinets or autoclaves, are all formulated around the prevention of contamination. There are various factors affecting pollution, such as the type of explant, season and time of material collection, top treatment method, type and concentration of disinfectant, disinfection time and disinfection method; medium and vessel sterilization, operators, workers The requirements of the environment and the working quality of the ultra-clean workbench are closely related to pollution. Therefore, the problem of pollution control is very complicated and expensive, which has become one of the important reasons restricting the industrialization of tissue culture, and also greatly limits the application range of tissue culture technology, which is not conducive to the promotion and popularization in conventional agricultural production. Therefore, simplifying tissue culture techniques and preventing contamination have become indispensable tasks in tissue culture research.

At present, sugarcane tissue culture propagation technology is still carried out in the traditional way.

Contents of the invention

The object of the present invention is to provide a method for rooting culture of sugarcane tissue culture seedlings. The medium does not contain exogenous hormones, does not require high-pressure sterilization, and inoculation does not require the use of ultra-clean workbenches. The operation is simple and fast, and the pollution rate is low. Reduce the rooting culture cost by 40-60%; the cultivated rooting seedlings grow robustly.

The present invention achieves the above object through the following technical solutions: a method for rooting and culturing of sugarcane tissue cultured seedlings, comprising the steps of: inserting the multiplied aseptic tissue cultured seedlings into MS+sodium hypochlorite 800-1200mg/L+white sugar 30g/L medium, sealing , placed in the culture room for rooting culture for 20-30 days to grow a complete root system.

The temperature of the culture room is 27±1° C., and the light is 12-16 hours per day.

The MS is the plant tissue culture medium disclosed by Murashige and Skoog in 1962;

Its chemical formula of described sodium hypochlorite is NaClO.

The culture medium does not contain exogenous hormones and does not need to be sterilized by high pressure.

The outstanding technical effects of the present invention are:

The rooting of sugarcane tissue culture seedlings does not need to add exogenous hormones, the medium does not need to be sterilized by high pressure, and the inoculation does not require the use of ultra-clean workbench, which fundamentally simplifies the tissue culture process, simple and fast operation, low pollution rate, and reduces the cost of rooting culture 40-60%; the cultivated rooted seedlings grow vigorously.

Detailed ways

The technical solution of the present invention will be further described below through specific examples.

Example 1

The method for rooting culture of sugarcane tissue culture seedlings of the present invention comprises the steps:

1. Culture Vessel Preparation

Use the existing tissue culture container as the basic container, rinse and dry the water in the container naturally for later use;

2. Medium Preparation

Take tap water to prepare MS + sodium hypochlorite 8000mg/L + white sugar 30g/L liquid medium, and then divide it into culture containers according to a certain amount, seal it, and set it aside.

3. Rooting culture

Put the tissue-cultured seedlings into the above-mentioned culture medium and place them in a culture room for cultivation. After 20-30 days of cultivation, the tissue-cultured seedlings can grow into a complete root system.

4. Implementation effect

Example 2

Another example of the method for sugarcane tissue culture seedling rooting culture of the present invention, comprises the steps:

1. Culture Vessel Preparation

Use the existing tissue culture container as the basic container, rinse and dry the water in the container naturally for later use;

2. Medium Preparation

Weigh 6-8 grams of agar per liter of medium and boil 200ml of tap water, prepare MS + sodium hypochlorite 1200mg/L + white sugar 30g/L medium, mix well with tap water, and pack in a certain amount in culture containers before the medium solidifies , seal and set aside.

3. Rooting culture

Put the tissue-cultured seedlings into the above-mentioned culture medium and place them in a culture room for cultivation. After 20-30 days of cultivation, the tissue-cultured seedlings can grow into a complete root system.

4. Implementation effect

Claims (1)

1. A method for rooting culture of sugarcane tissue culture seedlings, characterized in that: the proliferation of aseptic tissue culture seedlings is inserted in the MS+sodium hypochlorite 800-1200mg/L+white sugar 30g/L medium, sealed, and placed in a culture room temperature of 27 ±1°C, 12-16 hours of light per day for rooting culture for 20-30 days to grow a complete root system. the