CN103319272B - A kind of practical white gold needle mushroom substratum and collocation method thereof - Google Patents
A kind of practical white gold needle mushroom substratum and collocation method thereof Download PDFInfo
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Abstract
The invention discloses a kind of practical white gold needle mushroom substratum and its collocation method, formula and the weight ratio of described substratum are as follows: the dried silkworm chrysalis meal of 10%-15%, the bagasse of 20%-35%, the wheat bran of 3%-5%, the Zulkovsky starch of 10%-15%, the urea of 1%-3%, the mixing inorganics of 10%-20%, the gypsum of 1%-3%; The white gold needle mushroom growth that the present invention cultivates is vigorous, and the body of rod of bacterial strain not only sturdy but also length is greater than common needle mushroom; And manufacture and production cost lower, improve the economic benefit of needle mushroom; Shorten the growth time of needle mushroom, improve the resistance of white gold needle mushroom.
Description
Technical field
The present invention relates to mushroom substratum and preparation method thereof, particularly relate to a kind of practical white gold needle mushroom substratum and collocation method thereof.
Background technology
White gold needle mushroom is the white mutation of needle mushroom, and entire body is pure white.Because needle mushroom sporophore occurs at a lower temperature, have another name called dried mushroom.Separately there are structure bacterium, plain mushroom, hair handle money bacterium, freeze bacterium etc. and be commonly called as.The amino acid whose content of needle mushroom is very abundant, and higher than general mushroom class, especially the content of Methionin is high especially, and Methionin has the function promoting children ' s intelligence development.Containing protein 8.87% in needle mushroom dry product, carbohydrate 60.2%, robust fibre reaches 7.4%, often eats and can prevent and treat Peptic Ulcers.Have research to show again, a kind of material contained in needle mushroom has good antitumous effect.
The gross protein value of dried silkworm chrysalis meal is high, is respectively 54%, and wherein chitin state nitrogen is approximately about 4%.Its amino acid compositing characteristic is that methionine content is very high, is respectively 2.2%.Lysine content is also fine, substantially equal with imported fish meal.Tryptophane is also high, and up to 1.25%-1.5%, because the protein content in dried silkworm chrysalis meal is higher, through it through resolving into multiple amino acids, multiple amino acids facilitates the growth of needle mushroom through resolution; Wherein Methionin is that white gold needle mushroom grows necessary amino acid, rich content.
Needle mushroom artificial cultivation method is simple, as long as can control envrionment conditions well, just easily obtaining reliable and stable output, is one of stable approach of getting rich of peasant; Nitrogenous source, carbon source, inorganic salt etc. are needed to cultivate key element in its substratum, what the nitrogenous source of daily cultivation needle mushroom was selected is corn cob or cotton seed hulls, nutritive value and the composition of the needle mushroom grown out are less, and composition is single, are unfavorable for the eutrophic absorption of people.
Summary of the invention
For above-mentioned Problems existing, the practical white gold needle mushroom substratum that the object of the invention is to provide a kind of cultivation effect good and collocation method thereof.
In order to achieve the above object, the technical solution used in the present invention is as follows: a kind of practical white gold needle mushroom substratum, formula and the weight ratio of described substratum are as follows: the dried silkworm chrysalis meal of 10%-15%, the bagasse of 20%-35%, the wheat bran of 3%-5%, the Zulkovsky starch of 10%-15%, the urea of 1%-3%, the mixing inorganics of 10%-20%, the gypsum of 1%-3%.
Dried silkworm chrysalis meal of the present invention is silkworm chrysalis drying, the pressed powder pulverizing rear support, described dried silkworm chrysalis meal is the solia particle of diameter 1-5mm, the water content of described dried silkworm chrysalis meal is 8%-10%, containing a small amount of moisture in the particle of dried silkworm chrysalis meal, mixing adhesion effect is good, and dried silkworm chrysalis meal easily resolves into the amino acid that needle mushroom easily grows in water, improve assimilated efficiency.
Mixing inorganics of the present invention be pearlstone and Ovum crusta Gallus domesticus by the mixture mixed, the mixed weight of described pearlstone and Ovum crusta Gallus domesticus than being 2:1, described Ovum crusta Gallus domesticus be stir pulverize after be in the solid particulate of surface diameter 1-2cm; Define in the medium between certain interstice coverage, facilitate the respiration of needle mushroom root system.
Containing moisture in substratum of the present invention, described water is distilled water, and the weight ratio of moisture is with substratum gross weight than being 2:1, and moisture is not only one of necessary factor of Growth of Flammulina Velutipes, and not containing microorganisms such as other bacteriums in distilled water, ensure that the gnotobasis of substratum.
The epidermis particle that wheat bran of the present invention is wheat after fluffing machine processing, after high pressure steam is boiled, after double gauze filters gained filter residue, after oven dry aseptic wheat bran; Wheat bran particle after production is comparatively thick, convenient mixing and utility program, and ensures the gnotobasis of substratum.
Zulkovsky starch of the present invention is by one or both mixtures in sweet potato starch and potato starch; The particle of sweet potato starch is comparatively thick, easily dry, and convenient absorption is shaped with mixing; The dried weight ratio of described wheat bran and Zulkovsky starch is 1:3, and wheat bran and Zulkovsky starch are as the secondary N source of substratum and C source, and nutritive element enriches, and conveniently absorbs and the growth of needle mushroom.
Practical white gold needle mushroom substratum of the present invention the preferred weight percent proportioning of each composition be: dried silkworm chrysalis meal 15%, bagasse 28%, wheat bran 3.5%; Zulkovsky starch 10.5%, urea 2.5%, mixed inorganic: expanded pearlite salt: 12%; Ovum crusta Gallus domesticus 6%, gypsum 2%; The percent mass proportioning of the distilled water wherein mixed is 159%.
Urea of the present invention is the chemical fertilizer granule of 0.5-1cm, and urea provides other inorganic N sources for white gold needle mushroom growth, improves the growth efficiency of needle mushroom.
Gypsum of the present invention is plaster stone through calcining and levigate white lime powder, described powder mean particle dia be 1-5mm, mix mixing liquid afterwards with water and easily enter between each moiety of substratum, facilitate the sizing of substratum.
A kind of collocation method of practical platinum needle mushroom culture medium:
1) be that 10%-15% dried silkworm chrysalis meal and 10%-15% Zulkovsky starch fully mix 1-3h through stirrer by weight ratio, after stirring completes, by the filter screen structure of mixed powder by 5mm, obtain the mixed powder particle that diameter is 1-5mm, mixed powder particle is stand-by after weighing;
2) by weight ratio 3%-5% wheat bran and and 20%-35% bagasse through stirrer crushing, stirring 1-2h, weigh after high temperature drying process after having stirred; Dried wheat bran and bagasse decrease the impact of moisture factor when configuring substratum,
3) in stirrer, add the wheat bran after high temperature drying and sugarcane mixture, under the condition stirred, divide the mixture adding dried silkworm chrysalis meal and Zulkovsky starch for three times, added once every 15 minutes, add after 45 minutes, after adding, be uniformly mixed 1h, to reach well-mixed effect;
4) mixed powder and immobilized particles are divided into many groups, simultaneously through high-pressure steam sterilizing pan sterilizing 12h, improve sterilizing efficiency, sterilising temp is 100 DEG C-110 DEG C;
5), after Ovum crusta Gallus domesticus and pearlstone being mixed according to the weight ratio of 1:2, because the particle of pearlstone is slightly large, 1-2h is uniformly mixed through upset stirrer, mixing fully, add boiling sterilization in moisture Autoclave after having mixed, boiling sterilization 5-6h, weigh after sterilizing completes stand-by;
6) the blended solid powder after sterilizing and mixing inorganics is uniformly mixed, add weight ratio is in the mixture 1%-3% urea fertilizer particle simultaneously, the distilled water of gross weight than 1/2nd is added after mixing, while being uniformly mixed, the dilute hydrochloric acid of a small amount of saturated solution of sodium bicarbonate and 0.05mol/L is used to regulate the PH to 6.5-7.0 of mixed culture medium;
7) gypsum and the distilled water of residue 1/2nd weight ratios are fully mixed into thick liquid, slowly be added drop-wise in mixture under the condition slowly stirred at large-scale stirrer, load in single port plastics bag, leave standstill and deposit 3h, sack is tightened, low-temperature storage under aseptic condition after light pressure.
The better collocation method of practical function of the present invention is: 1) be that 15% dried silkworm chrysalis meal and 10.5% Zulkovsky starch fully mix 2h through stirrer by weight ratio, after stirring completes, by the filter screen structure of mixed powder by 5mm, obtain the mixed powder particle that diameter is 1-5mm, mixed powder particle is stand-by after weighing;
2) by weight ratio 3.5% wheat bran and and 28% bagasse through stirrer crushing, stirring 1.5h, weigh after high temperature drying process after having stirred;
3) in stirrer, add the wheat bran after high temperature drying and sugarcane mixture, under the condition stirred, divide the mixture adding dried silkworm chrysalis meal and Zulkovsky starch for three times, added once every 15 minutes, add after 45 minutes, after adding, be uniformly mixed 1h, to reach well-mixed effect;
4) mixed powder and immobilized particles are divided into many groups, simultaneously through high-pressure steam sterilizing pan sterilizing 12h, improve sterilizing efficiency, sterilising temp is 105 DEG C;
5), after Ovum crusta Gallus domesticus and pearlstone being mixed according to the weight ratio of 1:2, because the particle of pearlstone is slightly large, 1h is uniformly mixed through upset stirrer, mixing fully, add boiling sterilization in moisture Autoclave after having mixed, boiling sterilization 6h, weigh after sterilizing completes stand-by;
6) the blended solid powder after sterilizing and mixing inorganics is uniformly mixed, add weight ratio is in the mixture 2.5% urea fertilizer particle simultaneously, the distilled water that mass ratio is 79.5% is added after mixing, while being uniformly mixed, the dilute hydrochloric acid of a small amount of saturated solution of sodium bicarbonate and 0.05mol/L is used to regulate the PH to 6.7 of mixed culture medium;
7) distilled water being 79.5% by gypsum and mass ratio is fully mixed into thick liquid, slowly be added drop-wise in mixture under the condition slowly stirred at large-scale stirrer, load in single port plastics bag, leave standstill and deposit 3h, sack is tightened, low-temperature storage under aseptic condition after light pressure.
The invention has the advantages that: the present invention adopt the cultural method of white gold needle mushroom to utilize the higher particularly lysine content of protein content high dried silkworm chrysalis meal as substratum body material; And Methionin to be white gold needle mushroom grow necessary amino acid, therefore white gold needle mushroom growth is vigorous, and the body of rod of bacterial strain not only sturdy but also length is greater than common needle mushroom; Select bagasse as the secondary feedstock of substratum, utilization of waste material simultaneously, manufacture and production cost lower, improve the economic benefit of needle mushroom; Select the carrier making pearlstone Ovum crusta Gallus domesticus as substratum, inorganic salt content enriches simultaneously, defines in the medium between certain interstice coverage simultaneously, facilitates the respiration of needle mushroom root system, improves the growth efficiency of needle mushroom; The particle of the material that the present invention selects is less, facilitates the abundant mixing between raw material, mixes, facilitate needle mushroom Root Absorption, shorten the growth time of needle mushroom, improve the resistance of white gold needle mushroom.
Embodiment
Below in conjunction with embodiment, the present invention is described in further detail.
embodiment 1:a kind of practical white gold needle mushroom substratum, formula and the weight ratio of described substratum are as follows: the dried silkworm chrysalis meal of 10%-15%, the bagasse of 20%-35%, the wheat bran of 3%-5%, the Zulkovsky starch of 10%-15%, the urea of 1%-3%, the mixing inorganics of 10%-20%, the gypsum of 1%-3%.Described dried silkworm chrysalis meal is silkworm chrysalis drying, the pressed powder pulverizing rear support, described dried silkworm chrysalis meal is the solia particle of diameter 1-5mm, the water content of described dried silkworm chrysalis meal is 8%-10%, containing a small amount of moisture in the particle of dried silkworm chrysalis meal, mixing adhesion effect is good, and dried silkworm chrysalis meal easily resolves into the amino acid that needle mushroom easily grows in water, improve assimilated efficiency.Mixing inorganics be pearlstone and Ovum crusta Gallus domesticus by the mixture mixed, the mixed weight of described pearlstone and Ovum crusta Gallus domesticus than being 2:1, described Ovum crusta Gallus domesticus be stir pulverize after be in the solid particulate of surface diameter 1-2cm; Define in the medium between certain interstice coverage, facilitate the respiration of needle mushroom root system.Containing moisture in described substratum, described water is distilled water, and the weight ratio of moisture is with substratum gross weight than being 2:1, and moisture is not only one of necessary factor of Growth of Flammulina Velutipes, and not containing microorganisms such as other bacteriums in distilled water, ensure that the gnotobasis of substratum.The epidermis particle that described wheat bran is wheat after fluffing machine processing, after high pressure steam is boiled, after double gauze filters gained filter residue, after oven dry aseptic wheat bran; Wheat bran particle after production is comparatively thick, convenient mixing and utility program, and ensures the gnotobasis of substratum.Described Zulkovsky starch is by one or both mixtures in sweet potato starch and potato starch; The particle of sweet potato starch is comparatively thick, easily dry, and convenient absorption is shaped with mixing.The dried weight ratio of described wheat bran and Zulkovsky starch is 1:3, and wheat bran and Zulkovsky starch are as the secondary N source of substratum and C source, and nutritive element enriches, and conveniently absorbs and the growth of needle mushroom.Described urea is the chemical fertilizer granule of 0.5-1cm, and urea provides other inorganic N sources for white gold needle mushroom growth, improves the growth efficiency of needle mushroom.Described gypsum is plaster stone through calcining and levigate white lime powder, described powder mean particle dia be 1-5mm, mix mixing liquid afterwards with water and easily enter between each moiety of substratum, facilitate the sizing of substratum.
embodiment 2:to corn cob conventional on the market and peptone, to be the formulated substratum of body material be that body material carries out that comparative test result is as shown in the table:
Practical white gold needle mushroom substratum of the present invention the preferred weight percent proportioning of each composition be: dried silkworm chrysalis meal 15%, bagasse 28%, wheat bran 3.5%; Zulkovsky starch 10.5%, urea 2.5%, mixed inorganic: expanded pearlite salt: 12%; Ovum crusta Gallus domesticus 6%, gypsum 2%; The percent mass proportioning of the distilled water wherein mixed is 159%; The substratum be made into according to this proportioning and the contrast experiment of ordinary culture medium dried silkworm chrysalis meal substratum of the present invention as shown in the table are not only shorter than other common substratum greatly on growth cycle, length and the protein content of its bacterial strain are higher, particularly the white gold needle mushroom cultivated compared with other substratum of lysine content is wherein high, the riotous growth of white gold needle mushroom, the resistance of bacterial strain is higher, the anatomic shape of overall bacterial strain, the more common bacterial strain of output is high.
embodiment 3:a kind of collocation method of practical platinum needle mushroom culture medium:
1) be that 15% dried silkworm chrysalis meal and 10.5% Zulkovsky starch fully mix 2h through stirrer by weight ratio, after having stirred, by the filter screen structure of mixed powder by 5mm, obtain the mixed powder particle that diameter is 1-5mm, mixed powder particle is stand-by after weighing;
2) by weight ratio 3.5% wheat bran and and 28% bagasse through stirrer crushing, stirring 1.5h, weigh after high temperature drying process after having stirred;
3) in stirrer, add the wheat bran after high temperature drying and sugarcane mixture, under the condition stirred, divide the mixture adding dried silkworm chrysalis meal and Zulkovsky starch for three times, added once every 15 minutes, add after 45 minutes, after adding, be uniformly mixed 1h, to reach well-mixed effect;
4) mixed powder and immobilized particles are divided into many groups, simultaneously through high-pressure steam sterilizing pan sterilizing 12h, improve sterilizing efficiency, sterilising temp is 105 DEG C;
5), after Ovum crusta Gallus domesticus and pearlstone being mixed according to the weight ratio of 1:2, because the particle of pearlstone is slightly large, 1h is uniformly mixed through upset stirrer, mixing fully, add boiling sterilization in moisture Autoclave after having mixed, boiling sterilization 6h, weigh after sterilizing completes stand-by;
6) the blended solid powder after sterilizing and mixing inorganics is uniformly mixed, add weight ratio is in the mixture 2.5% urea fertilizer particle simultaneously, the distilled water that mass ratio is 79.5% is added after mixing, while being uniformly mixed, the dilute hydrochloric acid of a small amount of saturated solution of sodium bicarbonate and 0.05mol/L is used to regulate the PH to 6.7 of mixed culture medium;
7) distilled water being 79.5% by gypsum and mass ratio is fully mixed into thick liquid, slowly be added drop-wise in mixture under the condition slowly stirred at large-scale stirrer, load in single port plastics bag, leave standstill and deposit 3h, sack is tightened, low-temperature storage under aseptic condition after light pressure.
It should be noted that, above-mentioned is only preferred embodiment of the present invention, is not used for limiting protection scope of the present invention, and equivalents done on the basis of above-described embodiment all belongs to protection scope of the present invention.
Claims (7)
1. the collocation method of a practical white gold needle mushroom substratum, it is characterized in that, formula and the weight ratio percentage of described substratum are as follows: the dried silkworm chrysalis meal of 10%-15%, the bagasse of 20%-35%, the wheat bran of 3%-5%, the Zulkovsky starch of 10%-15%, the urea of 1%-3%, the mixed inorganic of 10%-20%, the gypsum of 1%-3%; Containing moisture in described substratum, described water is distilled water, and the collocation method of described substratum is as follows:
1) be that 10%-15% dried silkworm chrysalis meal and 10%-15% Zulkovsky starch fully mix 1-3h through stirrer by weight ratio, after stirring completes, by the filter screen structure of mixed powder by 5mm, obtain the mixed powder particle that diameter is 1-5mm, mixed powder particle is stand-by after weighing;
2) by weight ratio 3%-5% wheat bran and and 20%-35% bagasse through stirrer crushing, stirring 1-2h, weigh after high temperature drying process after having stirred;
3) in stirrer, add the wheat bran after high temperature drying and sugarcane mixture, under the condition stirred, divide the mixture adding dried silkworm chrysalis meal and Zulkovsky starch for three times, added once every 15 minutes, add after 45 minutes, after adding, be uniformly mixed 1h, to reach well-mixed effect;
4) mixed powder and immobilized particles are divided into many groups, simultaneously through high-pressure steam sterilizing pan sterilizing 12h, improve sterilizing efficiency, sterilising temp is 100 DEG C-110 DEG C;
5), after Ovum crusta Gallus domesticus and pearlstone being mixed according to the weight ratio of 1:2, because the particle of pearlstone is slightly large, 1-2h is uniformly mixed through upset stirrer, mixing fully, add boiling sterilization in moisture Autoclave after having mixed, boiling sterilization 5-6h, weigh after sterilizing completes stand-by;
6) whipping step 4) in mixed inorganic in blended solid powder after mixing sterilizing and step 5) after sterilizing, add weight ratio is in the mixture 1%-3% urea fertilizer particle simultaneously, the distilled water of gross weight than 1/2nd is added after mixing, while being uniformly mixed, the dilute hydrochloric acid of a small amount of saturated solution of sodium bicarbonate and 0.05mol/L is used to regulate the PH to 6.5-7.0 of mixed culture medium;
7) gypsum and the distilled water of residue 1/2nd weight ratios are fully mixed into thick liquid, slowly be added drop-wise in mixture under the condition that large-scale stirrer slowly stirs, load in single port plastics bag, leave standstill and deposit 3h, sack is tightened, low-temperature storage under aseptic condition after light pressure.
2. the collocation method of practical white gold needle mushroom substratum according to claim 1, it is characterized in that, described dried silkworm chrysalis meal is silkworm chrysalis drying, the pressed powder pulverizing rear support, and described dried silkworm chrysalis meal is the solia particle of diameter 1-5mm, and the water content of described dried silkworm chrysalis meal is 8%-10%.
3. the collocation method of practical white gold needle mushroom substratum according to claim 1, it is characterized in that, described mixed inorganic is that pearlstone and Ovum crusta Gallus domesticus are by the mixture mixed, described pearlstone is 2:1 with the mixed weight ratio of Ovum crusta Gallus domesticus, and described Ovum crusta Gallus domesticus is the solid particulate being in surface diameter 1-2cm after stirring pulverizing.
4. the collocation method of practical white gold needle mushroom substratum according to claim 1, it is characterized in that, described wheat bran is the epidermis particle of wheat after fluffing machine processing, after high pressure steam is boiled, through double gauze filter after gained filter residue, after oven dry aseptic wheat bran.
5. the collocation method of practical white gold needle mushroom substratum according to claim 1, it is characterized in that, described Zulkovsky starch is by one or both mixtures in sweet potato starch and potato starch, and the dried weight ratio of described wheat bran and Zulkovsky starch is 1:3.
6. the collocation method of practical white gold needle mushroom substratum according to claim 1, is characterized in that, described urea is the chemical fertilizer granule of 0.5-1cm after screening.
7. the collocation method of practical white gold needle mushroom substratum according to claim 1, is characterized in that, described gypsum is plaster stone through calcining and levigate white lime powder, described powder mean particle dia be 1-5mm.
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| CN105801236A (en) * | 2014-12-31 | 2016-07-27 | 韦照伟 | Needle mushroom bag produced from bagasse |
| CN106105774A (en) * | 2016-06-28 | 2016-11-16 | 安徽金豪生态农业科技有限公司 | A kind of breeding method of high-yield flammulina velutipes |
| CN109197388A (en) * | 2018-11-22 | 2019-01-15 | 山东友和菌业有限公司 | A kind of culture medium and preparation method of yellow needle mushroom |
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| CN102578371A (en) * | 2012-03-28 | 2012-07-18 | 常熟市汇康食品厂 | Method for producing mushroom powder for feeds |
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