CN103308587A - Hollow fiber electrophoresis gel imaging system - Google Patents

Abstract

The invention relates to a hollow-core optical fiber electrophoresis gel imaging system, which includes a CCD or CMOS camera capable of focusing, a stepping motor, a high-voltage power supply electrode, a first optical filter, a hollow-core optical fiber, a touch screen LED display, and a second optical filter film, ultraviolet lamp and imaging obscura; one or more hollow-core optical fibers are arranged between the two high-voltage power supply electrodes, and the camera is installed above the hollow-core optical fiber, driven by a stepping motor to move left and right horizontally, and the bottom of the camera is installed There is a first optical filter; the second optical filter is arranged under the hollow-core optical fiber, and the ultraviolet lamp is below the second optical filter; the touch-screen LED display is used to control the imaging system; the hollow-core optical fiber is a gel The biological or drug molecule sample to be tested is dropped on one end of the hollow-core optical fiber, and the hollow-core optical fiber is used as the electrophoresis swimming lane and the beam conduction channel to perform electrophoresis gel imaging and molecular spectral analysis and detection on the sample. The system has the characteristics of less sample consumption, high sensitivity and high efficiency, and no pollution.

Description

Hollow-core fiber optic electrophoresis gel imaging system

technical field

The present invention relates to a hollow-core optical fiber electrophoresis gel imaging system.

Background technique

Nucleic acid gel electrophoresis is a common method for separating, identifying and purifying nucleic acid molecules, probes and amplification products in molecular biology experiments. Its basic principle is to place the gel in an electric field, and use the characteristic of the charged nucleic acid at neutral pH to migrate to the anode through the gel mesh. After electrophoresis for an appropriate time, the nucleic acid fragments with different sizes and conformations will be arranged in a ladder shape. Gel in different positions, so as to achieve the purpose of separation. The migration rate of nucleic acid is affected by many factors such as molecular size, conformation, agarose concentration, applied voltage, electric field, electrophoresis buffer, and the amount of intercalating dye. Because of this, the effect of traditional nucleic acid electrophoresis imaging is affected by various experimental conditions and parameters, and it is difficult to achieve the organic unity of repeatability and accuracy. At the same time, ethidium bromide (EB), a commonly used dye for nucleic acid electrophoresis, is a strong mutagen and moderately toxic, and irregular use and operation may have adverse effects on human health and the environment.

Contents of the invention

In view of the defects existing in the prior art, the purpose of the present invention is to provide a hollow-core optical fiber electrophoresis gel imaging system, which integrates optical analysis detection and electrophoresis, and realizes integrated detection. This system is a "one-stop" experimental research platform integrating nucleic acid electrophoresis, optical imaging, data acquisition and analysis, and detection of biological nucleic acid molecules. It is used to determine the electrical, optical, and gel images of biomedical molecules, and to detect molecules in multiple directions. .

To achieve the above object, the present invention adopts the following technical solutions:

A hollow-core optical fiber electrophoresis gel imaging system, including a CCD or CMOS camera capable of focusing, a stepping motor, a high-voltage power supply electrode, a first optical filter, a hollow-core optical fiber, a touch-screen LED display, a second optical filter, and an ultraviolet Lamp and imaging obscura; one or more hollow-core optical fibers are arranged between the two high-voltage power supply electrodes, the camera is installed above the hollow-core optical fiber, and is driven by a stepping motor to move left and right horizontally, and the bottom of the camera is installed with a first Optical filter; the second optical filter is arranged below the hollow-core optical fiber, and the ultraviolet lamp is below the second optical filter; the camera, stepping motor, high-voltage power supply electrode, the first optical filter, and hollow-core optical fiber , the second optical filter and the ultraviolet lamp are placed in the imaging dark box, and the touch screen LED display is installed outside the imaging dark box to control the imaging system; the hollow-core optical fiber is a gel, and the biological or drug molecule sample drops It is added to one end of the hollow-core fiber, and the hollow-core fiber is used as the electrophoresis swimming lane and the beam conduction channel to perform electrophoresis gel imaging and molecular spectrum analysis and detection on the sample.

Compared with the prior art, the present invention has the following prominent substantive features and significant advantages:

The hollow-core optical fiber electrophoresis gel imaging system of the present invention can simultaneously detect molecules from multiple levels such as optical imaging, molecular spectrum analysis, and electrophoresis electric field separation. The main principle of this system is still the separation of charged nucleic acid molecules in the electrophoresis buffer. The difference is that the carrier of nucleic acid electrophoresis is a hollow fiber. On the one hand, the fiber can conduct light beams, and the information carried by the light beams after passing through the molecules can be detected. On the other hand, whether it can be used as an electrophoresis lane in the hollow to separate molecules by electric field action can effectively overcome the influence of nucleic acid molecules on the tube wall during electrophoresis, such as surface charge and surface activity. agents, proteins, etc. The dye molecule EB is directly poured into the fiber tube, avoiding contact with the outside world, which can effectively reduce pollution. The output signal is converted into a digital signal by the optical signal acquisition system, which converts the fluorescence intensity obtained by ultraviolet irradiation of embedded nucleic acid molecule dye EB, which can reduce the common background interference in traditional nucleic acid gel electrophoresis. In summary, the system has the characteristics of less sample consumption, high sensitivity and high efficiency, and no pollution.

Description of drawings

Figure 1 is a schematic diagram of the overall design of the novel gel imaging system involved in the present invention.

Detailed ways

Preferred embodiments of the present invention are described in detail as follows in conjunction with accompanying drawings:

Referring to Fig. 1, a hollow-core optical fiber electrophoretic gel imaging system includes a focusable CCD or CMOS camera 1, a stepping motor 2, a high-voltage power supply electrode 3, a first filter 4, a hollow-core optical fiber 5, and a touch screen LED A display 6, a second filter 7, an ultraviolet lamp 8 and an imaging dark box 9; one or more hollow-core optical fibers 5 are arranged in the middle of the two high-voltage power supply electrodes 3, and the camera 1 is installed above the hollow-core optical fibers 5 , driven by the stepping motor 2 to move left and right, the bottom of the camera 1 is equipped with a first filter 4; the second filter 7 is arranged below the hollow-core optical fiber 5, and the second filter 7 is an ultraviolet Lamp 8; the camera head 1, the stepping motor 2, the high-voltage power supply electrode 3, the first optical filter 4, the hollow-core optical fiber 5, the second optical filter 7 and the ultraviolet lamp 8 are placed in the imaging obscura 9, and the The touch-screen LED display 6 is installed on the outside of the imaging obscura 9 to control the imaging system; the hollow-core optical fiber 5 is a gel, and the biological or drug molecule sample to be measured is dripped on one end of the hollow-core optical fiber 5, and the hollow-core optical fiber 5 is used as The electrophoresis lane and the beam conduction channel are used to perform electrophoresis gel imaging and molecular spectrum analysis and detection on the sample.

In the present embodiment, the camera 1 adopts the APR-2110Z adjustable focus CCD camera of Sony Corporation of Japan; adopting a diameter of 420 μm hollow-core optical fiber 5, totally 20 pieces; the high-voltage power supply voltage is 1kv; the gel used is agarose gel; The test samples are DNA markers, that is, DNA molecular weight standards, and a PCR fragment of a phage in the phage display library. After the sample is added and electrophoresis is charged, a clear gel imaging picture is collected. the

Claims (1)

1. hollow-core fiber running gel imaging system, it is characterized in that, comprise CCD or the CMOS camera (1) that to focus, stepping motor (2), high-voltage power supply electrode (3), the first optical filter (4), hollow-core fiber (5), touch-screen light-emitting diode display (6), the second optical filter (7), uviol lamp (8) and imaging camera bellows (9); Described two high-voltage power supply electrodes (3) intermediate arrangement has one or more hollow-core fiber (5), described camera (1) is installed in the top of hollow-core fiber (5), drive left and right horizontal by stepping motor (2) and move, the bottom of camera (1) is equipped with the first optical filter (4); Described the second optical filter (7) is arranged in the below of hollow-core fiber (5), and the second optical filter (7) below is uviol lamp (8); Described camera (1), stepping motor (2), high-voltage power supply electrode (3), the first optical filter (4), hollow-core fiber (5), the second optical filter (7) and uviol lamp (8) place described imaging camera bellows (9), and described touch-screen light-emitting diode display (6) is installed in imaging camera bellows (9) outside, is used for controlling imaging system; Be gel in the hollow-core fiber (5), biology to be measured or drug molecule sample drop are added in an end of hollow-core fiber (5), utilize hollow-core fiber (5) as the swimming lane of electrophoresis and the passage of light beam conduction, sample is carried out running gel imaging and Molecular Spectral Analysis detection.

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