CN103304298A - Hericium erinaceus mother strain culture medium and making method thereof - Google Patents
Hericium erinaceus mother strain culture medium and making method thereof Download PDFInfo
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- CN103304298A CN103304298A CN2013102379361A CN201310237936A CN103304298A CN 103304298 A CN103304298 A CN 103304298A CN 2013102379361 A CN2013102379361 A CN 2013102379361A CN 201310237936 A CN201310237936 A CN 201310237936A CN 103304298 A CN103304298 A CN 103304298A
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Abstract
The invention relates to a hericium erinaceus mother strain culture medium. The hericium erinaceus mother strain culture medium is characterized in that melissyl alcohol with certain concentration is added to a PDA (Potato Dextrose Agar) enriched medium. The invention also relates to a making method of the hericium erinaceus mother strain culture medium. Compared with an existing PDA culture medium, the hericium erinaceus mother strain culture medium disclosed by the invention has the advantages that the growth vitality of hericium erinaceus mycelium is enhanced because the melissyl alcohol stimulates the energy metabolism of mycelium, so that the fungi culture time of a mother strain is shortened, the fungi culture time of an original strain is also shortened by making a hericium erinaceus original strain by using the mother strain, therefore, the differentiation phenomenon of a sporocarp during a strain germination period is avoided, the nutrient loss is reduced and the strain quality is improved.
Description
Technical field
Content of the present invention comprises Hericium erinaceus (Bull. Ex Fr.) Pers. Mother culture based formulas, and the making method of this substratum, belongs to the edible mashroom cultivating technical field.
Background technology
Hericium erinaceus (Bull. Ex Fr.) Pers., sporophore is round and thick, is covered with needle-like bacterium thorn, and the pole of figure is like the head of monkey, so gain the name.Hericium erinaceus (Bull. Ex Fr.) Pers. has the title of mountain delicacy, is a kind of dual-purpose bacterium of food, medicine of top grade.It can cure the diseases such as maldigestion, stomach ulcer and neurasthenia.The recent domestic medical evidence, Hericium erinaceus (Bull. Ex Fr.) Pers. has preferably auxiliary curative effect to the malignant tumour of the Digestive tract such as cancer of the stomach, esophagus cancer, with its good medicine of making monkey mushroom sheet etc. is arranged.
Growing of Hericium erinaceus (Bull. Ex Fr.) Pers., stricter to the requirement of nutritional condition, under different nutritional conditions, the power of mycelial growth, dense, speed difference is very large.Especially the technical difficulty that bacterial classification is made in the Hericium erinaceus (Bull. Ex Fr.) Pers. scale operation is larger, because the quality of bacterial classification is unstable, causes Hericium erinaceus (Bull. Ex Fr.) Pers. output also very unstable.
Make the female kind of Hericium erinaceus (Bull. Ex Fr.) Pers. with the PDA substratum of routine, the irregular pallial siphuncle of the normal formation of mycelia, the speed of growth is very slow, mycelia quantity seldom, need the above cultivation through 20d, mycelia just can be covered with the test tube slant, and this moment, formed a lot of goose pimples-fruit body primordiums on the inclined-plane, namely catabiosis has appearred in bacterial classification.After planting access hedgehog hydnum original seed with this mother, mycelial growth is sparse, very thin, skewness, often mycelia not yet send out full half, culture material just begins to form former base, causes nutrient to consume in a large number, spawn activity descends.
Triacontanol price quote is a kind of natural, nontoxic, efficient plant-growth regulator, uses trace level and just can produce appreciable results, and can improve the plurality of enzymes activity, promotes the effect of carbon nitrogen metabolism.
The present invention adopts the PDA enriched medium, adds certain density triacontanol price quote, stimulates the mycelium energy metabolism, has accelerated absorption, the conversion of nutritive substance, and Hericium erinaceus (Bull. Ex Fr.) Pers. filament length speed is greatly accelerated and is dense pure white, no longer forms shoestring and former base.Behind this bacterial classification access hedgehog hydnum original seed, mycelial growth is vigorous, healthy and strong, and the full bottle of bacterial classification time shorten has improved strain quality.
Summary of the invention
The present invention has following two aspects:
1, a kind of Hericium erinaceus (Bull. Ex Fr.) Pers. mother culture media
2, the making method of substratum
The technical solution adopted in the present invention is:
A kind of Hericium erinaceus (Bull. Ex Fr.) Pers. Mother culture based formulas: PDA enriched medium (potato 200g, glucose 20g, potassium primary phosphate 3g, agar 20g, tap water 1000ml), add substratum total mass 0.2mg/kg triacontanol price quote again.
Triacontanol price quote: be content 0.1% microemulsion, 0.1% triacontanol price quote stoste with 100 times of sterilized water dilutions, is got 10mg/kg dilution mother liquor, then use the membrane filter appts filtration sterilization.
Potassium primary phosphate: be chemically pure reagent.
The making method of substratum comprises the steps:
1. the potato wash clean, eye is dug up in peeling, takes by weighing 200g at balance, is cut into the fritter of 1cm-2cm;
2. the potato that cuts is poured in the aluminum pot, add water 1000ml, the 15min-20min that reburns after boiling, crisp and not mashed as degree take potato;
3. use 4 layers of filtered through gauze, get filtrate, put into 20g agar, be heated to agar and dissolve fully, use again 4 layers of filtered through gauze;
4. filtrate adds water to 1000mL, adds glucose 20g, potassium primary phosphate 3g, and heating is dissolved, and stirs;
5. be sub-packed in while hot in the test tube of Φ 20mm * 200mm, every test tube minute loading amount is 5ml, then cotton plug beyond the Great Wall;
6. autoclaving, pressure rises to 1.05kg/cm
2, temperature reaches 121 ℃-126 ℃, keeps 20min-30min, after taking the dish out of the pot, puts into Bechtop, slightly cools off;
7. treat that substratum is cooled to about 70 ℃, every test tube adds the triacontanol price quote dilution mother liquor 100 μ l after the filtration sterilization, and the liquid-transfering gun of crossing with sterilising treatment adds, and then shakes up (substratum not being splashed cotton plug), puts while hot the inclined-plane;
8. after the inoculation, 20 ℃-22 ℃ of culture temperature are cultivated through 7d-8d, and hericium mycelium covers with the inclined-plane.
With the female access Primary spawn material of planting of this hedgehog hydnum, owing to containing the plant hormone triacontanol price quote in the Mother culture base-material, stimulated mycelial growth, mycelial growth is vigorous, the full bottle of original seed time shorten.
The female kind of a kind of Hericium erinaceus (Bull. Ex Fr.) Pers. provided by the invention made novel method, in the PDA enriched medium, add triacontanol price quote, owing to role of phytohormone, strengthened the growth vigor of hericium mycelium, make female cultivation time shorten of planting, plant making Hericium erinaceus (Bull. Ex Fr.) Pers. original seed with this mother, the cultivation time of original seed shortens too, has avoided bacteria developing period sporophore differentiating phenomenon, reduce the loss of nutrient, improved the quality of bacterial classification.
Embodiment
A kind of Hericium erinaceus (Bull. Ex Fr.) Pers. Mother culture based formulas in the present embodiment: potato 200g, glucose 20g, potassium primary phosphate 3g, agar 20g, tap water 1000ml adds substratum (finished product) total mass 0.2mg/kg triacontanol price quote again.
Making method:
1. the potato wash clean, eye is dug up in peeling, takes by weighing 200g at balance, is cut into the fritter of 1cm-2cm;
2. the potato that cuts is poured in the aluminum pot, add water 1000ml, the 20min that reburns after boiling, crisp and not mashed as degree take potato;
3. use 4 layers of filtered through gauze, get filtrate, put into 20g agar, be heated to agar and dissolve fully, use again 4 layers of filtered through gauze;
4. filtrate adds water to 1000mL, adds glucose 20g, potassium primary phosphate 3g, and heating is dissolved, and stirs;
5. be sub-packed in while hot in the test tube of Φ 20mm * 200mm, every test tube minute loading amount is 5ml, then cotton plug beyond the Great Wall;
6. autoclaving, pressure rises to 1.05kg/cm
2, temperature reaches 125 ℃, keeps 25min, after taking the dish out of the pot, puts into Bechtop, slightly cools off;
7. in Bechtop, 0.1% triacontanol price quote stoste with 100 times of sterilized water dilutions, is got 10mg/kg dilution mother liquor, then use the membrane filter appts filtration sterilization;
8. treat that substratum is cooled to about 70 ℃, every test tube adds the triacontanol price quote dilution mother liquor 100 μ l after the filtration sterilization, and the liquid-transfering gun of crossing with sterilising treatment adds, and then shakes up (substratum not being splashed cotton plug), puts while hot the inclined-plane;
9. after the inoculation, 22 ℃ of culture temperature are cultivated through 7d, and hericium mycelium covers with the inclined-plane.
With the female access Primary spawn material of planting of this hedgehog hydnum, owing to containing the plant hormone triacontanol price quote in the Mother culture base-material, stimulated mycelial growth, mycelial growth is vigorous, and through the bacterium that sends out of 15d, mycelia sends out completely full bottle.
Claims (5)
1. a Hericium erinaceus (Bull. Ex Fr.) Pers. mother culture media is characterized in that containing following component and quality proportioning: PDA enriched medium (potato 200g, glucose 20g, potassium primary phosphate 3g, agar 20g, tap water 1000ml), add again substratum total mass 0.2mg/kg triacontanol price quote.
2. Hericium erinaceus (Bull. Ex Fr.) Pers. mother culture media according to claim 1, it is characterized in that described triacontanol price quote is content 0.1% microemulsion, 0.1% triacontanol price quote stoste with 100 times of sterilized water dilutions, is got 10mg/kg dilution mother liquor, then use the membrane filter appts filtration sterilization.
3. Hericium erinaceus (Bull. Ex Fr.) Pers. mother culture media according to claim 1 is characterized in that described potassium primary phosphate is chemically pure reagent.
4. the making method of the Hericium erinaceus (Bull. Ex Fr.) Pers. mother culture media described in the claim 1-3 is characterized in that:
1. the potato wash clean, eye is dug up in peeling, takes by weighing 200g at balance, is cut into the fritter of 1cm-2cm;
2. the potato that cuts is poured in the aluminum pot, add water 1000ml, the 15min-20min that reburns after boiling, crisp and not mashed as degree take potato;
3. use 4 layers of filtered through gauze, get filtrate, put into 20g agar, be heated to agar and dissolve fully, use again 4 layers of filtered through gauze;
4. filtrate adds water to 1000mL, adds glucose 20g, potassium primary phosphate 3g, and heating is dissolved, and stirs;
5. be sub-packed in while hot in the test tube of Φ 20mm * 200mm, every test tube minute loading amount is 5ml, then cotton plug beyond the Great Wall;
6. autoclaving, pressure rises to 1.05kg/cm
2, temperature reaches 121 ℃-126 ℃, keeps 20min-30min, after taking the dish out of the pot, puts into Bechtop, slightly cools off;
7. treat that substratum is cooled to about 70 ℃, every test tube adds the triacontanol price quote dilution mother liquor 100 μ l after the filtration sterilization, and the liquid-transfering gun of crossing with sterilising treatment adds, and then shakes up (substratum not being splashed cotton plug), puts while hot the inclined-plane;
8. after the inoculation, 20 ℃-22 ℃ of culture temperature are cultivated through 7d-8d, and hericium mycelium covers with the inclined-plane.
5. with the female access Primary spawn material of planting of the hedgehog hydnum described in the claim 1-4, owing to containing the plant hormone triacontanol price quote in the Mother culture base-material, stimulated mycelial growth, mycelial growth is vigorous, the full bottle of original seed time shorten.
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823710A (en) * | 2015-04-18 | 2015-08-12 | 余臣义 | Preparation method of hericium erinaceus mycelium powder |
| CN105130594A (en) * | 2015-08-10 | 2015-12-09 | 呼伦贝尔生态产业技术研究院 | Preparation method of high quality medium for edible-medicinal fungus first generation production mother culture and medium |
| CN105693330A (en) * | 2016-02-04 | 2016-06-22 | 湖南省宇秀生物科技有限公司 | High-transparency PDA (potato dextrose agar) culture medium preparing method |
| CN107691110A (en) * | 2017-11-28 | 2018-02-16 | 南宁市生润科技有限公司 | The breeding method of monkey mushroom bacterium |
Families Citing this family (1)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN111084054A (en) * | 2020-02-21 | 2020-05-01 | 铜仁学院 | Method for preparing hericium erinaceus strain from Huangjiang flower fungus material |
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| CN101683046A (en) * | 2008-09-22 | 2010-03-31 | 赵伟 | Method for preparing female seed of hericium erinaceum |
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| CN101971762A (en) * | 2010-02-09 | 2011-02-16 | 中国农业科学院农业资源与农业区划研究所 | Liquid submerged fermentation culture method for hericium erinaceus |
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2013
- 2013-06-02 CN CN201310237936.1A patent/CN103304298B/en not_active Expired - Fee Related
Patent Citations (4)
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| JPH03178901A (en) * | 1989-12-06 | 1991-08-02 | Teijin Ltd | Method for improving taste of rice grain |
| CN101683046A (en) * | 2008-09-22 | 2010-03-31 | 赵伟 | Method for preparing female seed of hericium erinaceum |
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Cited By (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN104823710A (en) * | 2015-04-18 | 2015-08-12 | 余臣义 | Preparation method of hericium erinaceus mycelium powder |
| CN105130594A (en) * | 2015-08-10 | 2015-12-09 | 呼伦贝尔生态产业技术研究院 | Preparation method of high quality medium for edible-medicinal fungus first generation production mother culture and medium |
| CN105693330A (en) * | 2016-02-04 | 2016-06-22 | 湖南省宇秀生物科技有限公司 | High-transparency PDA (potato dextrose agar) culture medium preparing method |
| CN107691110A (en) * | 2017-11-28 | 2018-02-16 | 南宁市生润科技有限公司 | The breeding method of monkey mushroom bacterium |
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