CN103265958A

CN103265958A - A kind of preparation method of commercialized organic material decomposing agent

Info

Publication number: CN103265958A
Application number: CN2013102281715A
Authority: CN
Inventors: 张新雄; 毛光平
Original assignee: Dongguan Baode Biological Engineering Co ltd
Current assignee: Dongguan Baode Biological Engineering Co ltd
Priority date: 2013-06-08
Filing date: 2013-06-08
Publication date: 2013-08-28
Also published as: CN103898008B; CN103898008A

Abstract

The invention relates to the technical field of agricultural microbial agent preparation processes, in particular to a preparation method of a commercial organic material decomposition agent, which comprises the following steps: (1) selecting and breeding strains and identifying the safety of the strains; (2) preparing a seed solution, including preparing a culture medium, preparing the seed solution and preparing a secondary seed solution; (3) stack retting fermentation, including the formulation of a stack retting fermentation medium and the setting of fermentation conditions; (4) drying, pulverizing, metering and packaging the piled product to obtain the organic material decomposing agent. The invention has the advantages of low production cost, simple and mature process, convenient use, obvious rotten application effect and contribution to general popularization and application.

Description

The preparation method of a kind of commercialization organic material composting agent

Technical field

The present invention relates to biological technical field, particularly the preparation method of a kind of commercialization organic material composting agent.

Background technology

In the prior art, the core microorganism of organic material composting agent generally carries out liquid or solid fermentation according to the difference of microorganism classification level, then with the goods of its microbial inoculum according to certain proportioning mix, technologies such as carrier absorption, drying.

At present, majority becomes thoroughly decomposed the agent product by the enlarged culturing to composite bacteria, by mixed fermentation or after single fermentation is mixed later fully simple the mixing, be a kind of product innovation of organic material composting agent, and do not relate to commercial categories such as the operative norm that relates in the evaluation index of organic material composting agent, quality surveillance, field demonstration popularization, lack the organic material composting agent in the exploration of aspects such as batch process, popularization, use, the potentiality of its patented product in the commercialization process are not done description.Its shortcoming is, the agent product that becomes thoroughly decomposed is pursued the quantity of functional bacterial classification, and its fermentation level and enzyme activity is not estimated, and causes and produces that zymotechnique is loaded down with trivial details, the production cycle long, viable count and enzyme activity level be uncertain; The strict relevant criterion of carrying out Ministry of Agriculture's formulation of goods; On using method, under different areas, different weather, the edatope function setting field test under the various crop straw directly returning to field pattern is not being verified.

Chinese patent application number is the patent of invention of 201010615828.X, discloses the agent of becoming thoroughly decomposed of a kind of stalk, comprises subtilis, Bacillus licheniformis bacillus, koning trichoderma, Phanerochaete chrysosporium and sporotrichum thermophile.Yet this documents only simply is mixed and made into the agent of becoming thoroughly decomposed with five kinds of bacterium, in actual production, complex manufacturing, inefficiency, production cost height, the performance index of the product of its preparation are not judged, in its further commercialization process, there is potential uncertain factor, and become thoroughly decomposed object and environment for use do not possess broad spectrum, and the agent result of use of becoming thoroughly decomposed that makes is relatively poor.

Yet, in the prior art, mode by stack retting is bred cultivation to the become thoroughly decomposed functional bacterial classification of agent of stalk, prepare a kind of starting material wide material sources, cheap, concise production process, number of viable is many, enzyme activity is vigorous and has the commodity organic material composting agent of broad spectrum effect, as yet report.

Summary of the invention

Problems such as that the object of the invention is is high at existing decomposing microbial inoculum technology production cost, complicated process of preparation, using method are loaded down with trivial details, effect instability, propose a kind of with low cost, technology succinct, conveniently use, the preparation method of obvious results commercialization organic material composting agent.

Purpose of the present invention is achieved through the following technical solutions.

The preparation method of a kind of commercialization organic material composting agent, it may further comprise the steps:

(1) strain improvement

A1, select bacterial classification: subtilis ( Bacillus subtilis), Bacillus licheniformis ( Bacillus licheniformis), the koning trichoderma bacterium ( Trichoderma koningii), Phanerochaete chrysosporium ( Phanerochete chrysosporium), the sporotrichum thermophile bacterium ( Sporotrichum thermophile).

Above bacterial classification is purchased respectively in Chinese microbial preservation management committee agricultural microorganism center (ACCC) and Chinese microbial preservation management committee common micro-organisms center (CGMCC), and its numbering is followed successively by ACCC 11089, CGMCC 1.0518 ACCC 30167, ACCC 30414, ACCC 30346.

The present invention selects the core microorganism of the mixing microorganisms product of the present invention that contains bacterium and fungi for use according to the moiety of lignocellulose and the feature of becoming thoroughly decomposed thereof.Under the conditions such as time and envrionment temperature of can becoming thoroughly decomposed in difference, the Mierocrystalline cellulose in the crop material, hemicellulose, xylogen are carried out orderly decomposition.Wherein subtilis and Bacillus licheniformis, metabolism produces cellulase; Koning trichoderma both can produce cellulase also can produce zytase; The metabolism of sporotrichum thermophile bacterium produces hemicellulase; The Phanerochaete chrysosporium metabolism produces lignoenzyme.

A2, strain bio security are identified: according to microbial fertilizer Biosafety current techique above-mentioned five kinds of bacterial classifications are carried out security and identify, remove poisonous unsafe bacterial strain.Above-mentioned five kinds of bacterial classifications are carried out horizontal security evaluation and screening, infected poisonous unsafe bacterial classification is removed, keep five bacterial classifications of no potential safety hazard, guarantee the organic material composting agent non-toxic and safe that makes.

Become thoroughly decomposed the biological safety authentication method of agent with reference to " microbial fertilizer Biosafety current techique general provisions " (NY 1109-2006), adopt the test of chmice acute per os, entrust Beijing Disease Prevention and Control Centre to identify that qualification result is as shown in the table.

Table 1: the bacterial classification security is identified.

As can be seen from the above table, the security qualification result of the agent of this organic material composting and five kinds of bacterial classifications selecting for use thereof is: nontoxic, no potential safety hazard can be used as agriculture microorganism core bacterial classification and breeds, uses.

(2) seed liquor preparation.

B1, slant culture.

B11, slant medium preparation

Subtilis slant medium (nutrient agar)-extractum carnis 5g/L, peptone 10g/L, sodium-chlor 5g/L, agar powder 20g/L, adjusting pH sterilize to neutral;

Bacillus licheniformis slant medium (nutrient agar)-extractum carnis 5g/L, peptone 10g/L, sodium-chlor 5g/L, agar powder 20g/L, adjusting pH sterilize to neutral;

Koning trichoderma bacterium slant medium-grass meal 60～80g/L, ammonium sulfate 25～35 g/L, potassium primary phosphate 4～8 g/L, sal epsom 1～2 g/L, calcium chloride 0.1～0.3 g/L, regulating pH is 6～7, adds agar 15g/L, 60～100 ℃ of heat fused, pour disk into, the cutting slivering of cooling back is put into the eggplant bottle, sterilization;

The effect of cooling back cutting slivering is: the grass meal in the substratum is met water and become dope, and is mobile poor, in the inclined-plane of can't directly packing into, so cooling slivering dress is easy to use in the inclined-plane of being convenient to pack into, and practicality.

Phanerochaete chrysosporium slant medium (wheat bran soaks juice-sucrose medium)-wheat bran 120～140g/L boils the back with filtered through gauze, sucrose 10～15g/L, pH nature, adds agar 20g/L, sterilization;

Sporotrichum thermophile bacterium slant medium (PDA slant medium)-peeling potato 200g/L is cut into small pieces and boils 15～20min, with four layers of filtered through gauze, with sucrose 20g/L, pH nature, adding agar 20g/L, sterilization.

B12, slant culture condition

After five kinds of thalline that above-mentioned test tube slant is cultivated dilute with 5～10ml sterilized water respectively, in the eggplant bottle, be inoculated in respectively in five kinds of slant mediums, culture condition is: culture temperature is 25～50 ℃, and incubation time is 24～96h.

B2, seed liquor preparation.

B21, primary seed solution preparation: the bacterial classification aseptic water washing with slant culture in above-mentioned five eggplant bottles is prepared into five kinds of uniform bacteria suspensions; The viable count of regulating every kind of bacterium is 80～12,000,000,000 cfu/ml, and the viable count of fungi is 0.5～300,000,000 cfu/ml; Be stored in respectively in the aseptic Steel Vessel, standby.

B22, secondary seed solution preparation:

The preparation of subtilis secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 150～170 parts in wheat bran, 40～60 parts on husk, 30～50 parts of Semen Maydis powder, 20～30 parts of dregs of beans, 1～3 part of sucrose, 0.5～1.5 part of potassium primary phosphate, 0. 5～1.5 parts of magnesium sulfate heptahydrates, 0.01～0.1 part of manganous sulfate, 4～6 parts of lime powders, 290～300 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned subtilis primary seed solution is inoculated shallow tray fermentation; Inoculate after 2～3 days, adopt colony counting method to measure the viable count in the tunning and regulate viable count with sterilized water and be the liquid seeds liquid of 80～12,000,000,000 cfu/ml, standby.

Wherein, the detailed measuring method of plate counting process can be with reference to " agricultural microorganism microbial inoculum " (GB 20287～2004).

Preferably, the subtilis culture condition is as shown in the table.

The preparation of Bacillus licheniformis secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 15～20 parts of dregs of beans, 40～55 parts of Semen Maydis powder, 200～240 parts in wheat bran, 4～6 parts of yeast powders, 0.5～1.5 part of potassium primary phosphate, 0.5～1.5 part of magnesium sulfate heptahydrate, 0.05～0.15 part of manganous sulfate, 12～16 parts in calcium carbonate, 5～6 parts of lime powders, 295～300 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned Bacillus licheniformis primary seed solution is inoculated shallow tray fermentation; Inoculate after 2～3 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 80～12,000,000,000 cfu/ml, standby.

Preferably, the Bacillus licheniformis culture condition is as shown in the table.

The preparation of koning trichoderma bacterium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 100～120 parts on husk, 60～80 parts in wheat bran, 4～6 parts in ammonium sulfate, 1～2 part of dipotassium hydrogen phosphate, 0.1～0.5 part of magnesium sulfate heptahydrate, 0.1～0.5 part of Calcium Chloride Powder Anhydrous, 200～230 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned koning trichoderma bacterium primary seed solution is inoculated shallow tray fermentation; Inoculate after 5～6 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 0.5～300,000,000 cfu/ml, standby.

Preferably, koning trichoderma bacterium culture condition is as shown in the table.

The preparation of Phanerochaete chrysosporium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 200～300 parts of wood chips, 400～600 parts on husk, 100～200 parts in wheat bran, 10～20 parts in ammonium sulfate, 5～10 parts of dipotassium hydrogen phosphates, 0.5～1 part of magnesium sulfate heptahydrate, 0.5～1 part of Calcium Chloride Powder Anhydrous, 200～250 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned Phanerochaete chrysosporium primary seed solution is inoculated shallow tray fermentation; Inoculate after 5～6 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 05～300,000,000 cfu/ml, standby.

Preferably, the Phanerochaete chrysosporium culture condition is as shown in the table.

The preparation of sporotrichum thermophile bacterium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 150～180 parts on husk, 20～40 parts in wheat bran, 3～5 parts in ammonium sulfate, 1～2 part of dipotassium hydrogen phosphate, 0.1～0.2 part of magnesium sulfate heptahydrate, 0.1～0.3 part of Calcium Chloride Powder Anhydrous, 200～240 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned sporotrichum thermophile bacterium primary seed solution is inoculated shallow tray fermentation; Inoculate after 5～6 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 0.5～300,000,000 cfu/ml, standby.

Preferably, sporotrichum thermophile bacterium culture condition is as shown in the table.

(3) pile fermentation.

C1, pile fermentation substratum

Comprise following raw materials by weight percent: wood chip 30～35%, straw powder 10～20%, husk 15～25%, wheat bran 20～25%, sucrose 0.5～1%, ammonium sulfate 3～5%, dipotassium hydrogen phosphate 0.3～0.5%, magnesium sulfate heptahydrate 0.1～0.3%, nature pH value, water material weight ratio is 1:1～4:1.

C2, stack retting method and initial incubation condition

Above-mentioned pile fermentation substratum is fully mixed in proportion, and the secondary seed solution after directly will diluting is sprayed onto the windrow surface, and secondary seed solution is evenly mixed with the pile fermentation substratum; Postvaccinal material is put into proving room to ferment.

C3, initial fermentation condition is set

Water content: regulating initial water content is 50～60%, contains seed liquor;

Initial pH value: natural pH value;

Initial temperature: natural temperature;

Inoculum size: the secondary seed solution of subtilis, Bacillus licheniformis, koning trichoderma bacterium, Phanerochaete chrysosporium and five kinds of bacterium of sporotrichum thermophile bacterium is evenly mixed according to the ratio of the weight ratio of 2.0～2.5:2.0～2.5:1.0～2.0:2.0～3.0:2.0～3.0, inoculate according to 5～10% mass ratioes;

Turning: will inoculate good material and be the placement of heap shape, and when the temperature of heap body rises to 60～80 ℃, carry out the turning cooling, and ferment turning for the first time the 3rd day; 4th, 5,6,7 days every turning in 24 hours once, the 8th～12 day, every turning in 48～72 hours once, make heap temperature remain on 25～50 ℃;

Particularly, the bottom surface of placing the fermenter that postvaccinal pile fermentation substratum uses arranges plastic floor, the wall material adopts the hard plastic board with the polyethylene material, conveniently carry out mechanize turning operation, according to the actual production space, can adjust quantity, shape, the size of fermenter flexibly, optimize usage space;

Air flow: regulate heap body air flow by the mode of punching, at heap body temperature raising period and between the pliotherm period, temperature 〉=35 ℃ feed the high-pressure air of 0.4～0.6 MPa, and air flow is per 6～12 hours each 10～20min of ventilation; Between heap body cooldown period, temperature≤30 ℃ feed the high-pressure air of 0.4～0.6 MPa, and air flow is ventilation in per 24～36 hours 5～10 minutes;

Method by the input high-pressure air in the fermenting process improves the inner oxygen content of heap body, is the growth of thalline, the oxygen that breeding provides capacity.Good supporting needs oxygen to keep its vital movement in the fermenting process; Regulate heap body air flow by the mode of punching.The drill rod that will have breather line inserts stack retting material bottom, feeds the high-pressure air of 0.4～0.6mpa;

Incubation time: 7～12 days.

(4) composting product drying, efflorescence, metering, packing

The dry temperature of heap soma is 40～60 ℃, be 6～10% to piling body water content, windrow is pulverized, crossed the 2mm sieve, it is standby to detect the back, its living bacteria count is 2.3～2,300,000,000/g, cellulase activity is 300～1000U/g, and the work of protein enzyme is 35～200 U/g, and moisture content is 2～30%, pH is 5.5～7.5, fineness 70～100%; Utilize that packaging facilities measures, package, namely make commercial organic material composting agent.

Require with reference to the clause in " organic material composting agent " (NY 609～2002): viable count 〉=0.5 hundred million/g, cellulase activity 〉=30U/g, protein enzyme live 〉=15, pH between 5-8, moisture≤35%, validity period 〉=12 month, and innoxious index all can claimed range within.Can be according to agrotype during use and become thoroughly decomposed to bear and require to add 20～40% wilkinite as stopping composition, suitably regulate every index, make its clause that all can satisfy in the standard require and can use.

With reference to " agricultural microorganism microbial inoculum " (GB 20287～2004) and " organic material composting agent " (NY 609～2002), adopt viable count and enzyme work thereof in colony counting method and three, the five～dinitrosalicylic acid colorimetric method for determining tunning to measure.

Measure viable count, cellulase activity and other physical and chemical indexs with reference to the method among " agricultural microorganism microbial inoculum " (GB 20287-2006), measurement result is as shown in the table.

From above-mentioned detected result as can be seen, its advantage of organic material composting agent that the present invention makes is: 1) operative norm of this product is " organic material composting agent " (NY 609～2002), above-mentioned all physical and chemical indexs all can satisfy or be better than relevant provision, and wherein living bacteria count is that 4.38 hundred million/g(clauses require 〉=0.5 hundred million/g), cellulase activity is 801.6U/g(clause requirement 〉=30 U/g), the pH value is 5. 8(clause requirement 5-8); 2) with reference to " agricultural microorganism microbial inoculum " (GB 20287-2006) added some new indexs, be 100%(clause requirement 〉=80% as fineness), the work of protein enzyme is 70.2 U/g(clause requirement 〉=15%), moisture 3.6%(clause requires≤35%).Can satisfy in national standard and the Ministry of Agriculture's standard clause requirement to the organic material composting agent simultaneously, and every testing index all satisfies or is higher than the clause requirement far away, it can be said that brightly, the present invention can draw a kind of basic demand that is better than relevant criterion under this manufacturing condition.

Wherein, described step B12, culture condition are specially:

The culture temperature of subtilis is 30～40 ℃, and incubation time is 24～48h;

The culture temperature of Bacillus licheniformis is 30～40 ℃, and incubation time is 24～48h;

The culture temperature of koning trichoderma bacterium is 25～32 ℃, and incubation time is 72～96h;

The culture temperature of Phanerochaete chrysosporium is 35～42 ℃, and incubation time is 72～96h;

The culture temperature of sporotrichum thermophile bacterium is 38～50 ℃, and incubation time is 72～96h.

Preferably, five kinds of culture of strains conditions such as following table.

Figure 2013102281715100002DEST_PATH_IMAGE016

Wherein, in the first order seed liquid preparing process of described step B21, the viable count of every kind of bacterium is 90～11,500,000,000 cfu/ml, and the viable count of fungi is 1～200,000,000 cfu/ml.

Wherein, the solid fermentation substratum of the subtilis in the preparation of the secondary seed solution of described step B22 comprises the raw material of following weight part: 155～165 parts in wheat bran, 45～55 parts on husk, 35～45 parts of Semen Maydis powder, 25～28 parts of dregs of beans, 1.5～2 parts of sucrose, 0.5～1 part of potassium primary phosphate, 0. 5～1 part of magnesium sulfate heptahydrate, 0.03～0.08 part of manganous sulfate, 4.5～5 parts of lime powders, 298 parts in water.

Wherein, the solid fermentation substratum of the Bacillus licheniformis in the preparation of the secondary seed solution of described step B22 comprises the raw material of following weight part: 16～18 parts of dregs of beans, 45～50 parts of Semen Maydis powder, 200～220 parts in wheat bran, 4.5～5.5 parts of yeast powders, 0.5～1 part of potassium primary phosphate, 0.5～1.5 part of magnesium sulfate heptahydrate, 0.05～0.1 part of manganous sulfate, 12～14 parts in calcium carbonate, 5.5 parts of lime powders, 300 parts in water.

Wherein, the solid fermentation substratum of koning trichoderma bacterium in the secondary seed solution preparation of described step B22 comprises the raw material of following weight part: 105～115 parts on husk, 65～70 parts in wheat bran, 4～5 parts in ammonium sulfate, 1～1.5 part of dipotassium hydrogen phosphate, 0.1～0.5 part of magnesium sulfate heptahydrate, 0.1～0.5 part of Calcium Chloride Powder Anhydrous, 220 parts in water.

Wherein, the solid fermentation substratum of the Phanerochaete chrysosporium in the preparation of the secondary seed solution of described step B22 comprises the raw material of following weight part: 240～280 parts of wood chips, 440～500 parts on husk, 80～160 parts in wheat bran, 13～17 parts in ammonium sulfate, 5～8 parts of dipotassium hydrogen phosphates, 0.5～0.8 part of magnesium sulfate heptahydrate, 0.6～1 part of Calcium Chloride Powder Anhydrous, 200～210 parts in water.

Wherein, the solid fermentation substratum of the sporotrichum thermophile bacterium in the preparation of the secondary seed solution of described step B22 comprises the raw material of following weight part: 160～170 parts on husk, 25～38 parts in wheat bran, 3～4 parts in ammonium sulfate, 1～1.5 part of dipotassium hydrogen phosphate, 0.1～0.2 part of magnesium sulfate heptahydrate, 0.1～0.3 part of Calcium Chloride Powder Anhydrous, 220 parts in water;

Wherein, the pile fermentation substratum comprises following raw materials by weight percent among the described C1: wood chip 33～35%, straw powder 12～15%, husk 20～25%, wheat bran 22～24%, sucrose 0.5～1%, ammonium sulfate 3～5%, dipotassium hydrogen phosphate 0.3～0.5%, magnesium sulfate heptahydrate 0.1～0.3%, natural Ph value, water material weight ratio are 1:1～2:1.

Wherein, in the inoculum size technology of step C3, the ratio of the weight ratio of the secondary seed solution of subtilis, Bacillus licheniformis, koning trichoderma bacterium, Phanerochaete chrysosporium and five kinds of bacterium of sporotrichum thermophile bacterium is 2:2.5:1:2.5:2, and secondary seed solution and pile fermentation substratum are inoculated with 6% mass ratio.

The invention has the beneficial effects as follows:

(1) prescription of substratum employing is reasonable, nutritious, can satisfy the culture propagation nutritional need.

(2) bacterial classification of the present invention adopts the test of chmice acute per os, and entrusts authoritative institution to identify, guarantees nontoxic, the no potential safety hazard of the bacterial classification of choosing, and can be used as agriculture microorganism core bacterial classification and breeds, uses, and is safe to use.

(3) substratum need not sterilization, direct raw material fermentation, concise production process, with low cost, production unit is simple.

(4) adopt becoming thoroughly decomposed of organic material composting agent that preparation technology of the present invention makes effective, the decomposition time is short.

(5) use as can be seen according to field test, organic material composting agent of the present invention can effectively improve soil organic matter content, raising inorganic nutrients content, promoting production increases income.

(6) the organic material composting agent that makes of present method commercialization production and selling at present, it can significantly promote the crop product, improves the economic return of crop, and has generally applied.

(7) its living bacteria count of organic material composting agent of making of the present invention is 2.3～2,300,000,000/g, cellulase activity is 300～1000U/g, the work of protein enzyme is 35～200 U/g, the physical and chemical index that is better than " organic material composting agent " (NY 609～2002) than index request is obvious is (clause requires: living bacteria count 〉=0.5 hundred million/g, cellulase activity 〉=30 U/g, protein enzyme live 〉=15% etc.) all, can satisfy in national standard and the Ministry of Agriculture's standard clause requirement to the organic material composting agent simultaneously.

Embodiment

The present invention is further illustrated below in conjunction with embodiment 1～4.

Embodiment 1.

The preparation method of a kind of commercialization organic material composting agent, it may further comprise the steps.

(1) strain improvement

A1, select bacterial classification: subtilis, Bacillus licheniformis bacillus, koning trichoderma bacterium, Phanerochaete chrysosporium, sporotrichum thermophile bacterium;

A2, strain bio security are identified: according to microbial fertilizer Biosafety current techique above-mentioned five kinds of bacterial classifications are carried out security and identify, remove poisonous unsafe bacterial strain.

(2) seed liquor preparation

B1, slant culture

B11, slant medium preparation

Subtilis slant medium-extractum carnis 5g/L, peptone 10g/L, sodium-chlor 5g/L, agar powder 20g/L, adjusting pH sterilize to neutral;

Bacillus licheniformis slant medium-extractum carnis 5g/L, peptone 10g/L, sodium-chlor 5g/L, agar powder 20g/L, adjusting pH sterilize to neutral;

Koning trichoderma bacterium slant medium-grass meal 60g/L, ammonium sulfate 25g/L, potassium primary phosphate 4g/L, sal epsom 1g/L, calcium chloride 0.1 g/L, regulating pH is 6, adds agar 15g/L, 60 ℃ of heat fused, pour disk into, the cutting slivering of cooling back is put into the eggplant bottle, sterilization;

Phanerochaete chrysosporium slant medium-wheat bran 120g/L boils the back with filtered through gauze, sucrose 10g/L, pH nature, adds agar 20g/L, sterilization;

Sporotrichum thermophile bacterium slant medium-peeling potato 200g/L is cut into small pieces and boils 15min, with four layers of filtered through gauze, with sucrose 20g/L, pH nature, adding agar 20g/L, sterilization;

B12, slant culture condition

Five kinds of thalline that above-mentioned test tube slant is cultivated, are inoculated in respectively in five kinds of slant mediums in the eggplant bottle respectively with after the dilution of 5ml sterilized water, and culture condition is specially:

The culture temperature of subtilis is 30 ℃, and incubation time is 48h;

The culture temperature of Bacillus licheniformis is 30 ℃, and incubation time is 48h;

The culture temperature of koning trichoderma bacterium is 25 ℃, and incubation time is 96h;

The culture temperature of Phanerochaete chrysosporium is 35 ℃, and incubation time is 96h;

The culture temperature of sporotrichum thermophile bacterium is 38 ℃, and incubation time is 96h;

B2, seed liquor preparation

B21, primary seed solution preparation: the bacterial classification aseptic water washing with slant culture in above-mentioned five eggplant bottles is prepared into five kinds of uniform bacteria suspensions; The viable count of regulating every kind of bacterium is 8,000,000,000 cfu/ml, and the viable count of fungi is 0.5 hundred million cfu/ml; Be stored in respectively in the aseptic Steel Vessel, standby;

B22, secondary seed solution preparation:

The preparation of subtilis secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 150 parts in wheat bran, 40 parts on husk, 30 parts of Semen Maydis powder, 20 parts of dregs of beans, 1 part of sucrose, 0.5 part of potassium primary phosphate, 0. 5 parts of magnesium sulfate heptahydrates, 0.01 part of manganous sulfate, 4 parts of lime powders, 290 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned subtilis primary seed solution is inoculated shallow tray fermentation; Inoculate after 2 days, adopt colony counting method to measure the viable count in the tunning and regulate viable count with sterilized water and be the liquid seeds liquid of 8,000,000,000 cfu/ml, standby;

The preparation of Bacillus licheniformis secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 15 parts of dregs of beans, 40 parts of Semen Maydis powder, 200 parts in wheat bran, 4 parts of yeast powders, 0.5 part of potassium primary phosphate, 0.5 part of magnesium sulfate heptahydrate, 0.5 part of manganous sulfate, 12 parts in calcium carbonate, 5 parts of lime powders, 295 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned Bacillus licheniformis primary seed solution is inoculated shallow tray fermentation; Inoculate after 2 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 8,000,000,000 cfu/ml, standby;

The preparation of koning trichoderma bacterium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 100 parts on husk, 60 parts in wheat bran, 4 parts in ammonium sulfate, 1 part of dipotassium hydrogen phosphate, 0.1 part of magnesium sulfate heptahydrate, 0.1 part of Calcium Chloride Powder Anhydrous, 200 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned koning trichoderma bacterium primary seed solution is inoculated shallow tray fermentation; Inoculate after 5 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 0.5 hundred million cfu/ml, standby;

The preparation of Phanerochaete chrysosporium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 200 parts of wood chips, 400 parts on husk, 100 parts in wheat bran, 10 parts in ammonium sulfate, 5 parts of dipotassium hydrogen phosphates, 0.5 part of magnesium sulfate heptahydrate, 0.5 part of Calcium Chloride Powder Anhydrous, 200 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned Phanerochaete chrysosporium primary seed solution is inoculated shallow tray fermentation; Inoculate after 5 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 0.5 hundred million cfu/ml, standby;

The preparation of sporotrichum thermophile bacterium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 150 parts on husk, 20 parts in wheat bran, 3 parts in ammonium sulfate, 1 part of dipotassium hydrogen phosphate, 0.1 part of magnesium sulfate heptahydrate, 0.1 part of Calcium Chloride Powder Anhydrous, 200 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned sporotrichum thermophile bacterium primary seed solution is inoculated shallow tray fermentation; Inoculate after 5 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 0.5 hundred million cfu/ml, standby.

(3) pile fermentation

C1, pile fermentation substratum

Comprise following raw materials by weight percent: wood chip 30%, straw powder 20%, husk 25%, wheat bran 20%, sucrose 0.5%, ammonium sulfate 4%, dipotassium hydrogen phosphate 0.4%, magnesium sulfate heptahydrate 0.1%, natural pH value, water material weight ratio is 1:1;

C2, stack retting method and initial incubation condition

Above-mentioned pile fermentation substratum is fully mixed in proportion, and the secondary seed solution after directly will diluting is sprayed onto the windrow surface, and secondary seed solution is evenly mixed with the pile fermentation substratum; Postvaccinal material is put into proving room to ferment;

C3, initial fermentation condition is set

Water content: regulating initial water content is 50%, contains seed liquor;

Initial pH value: natural pH value;

Initial temperature: natural temperature;

Inoculum size: the secondary seed solution of subtilis, Bacillus licheniformis, koning trichoderma bacterium, Phanerochaete chrysosporium and five kinds of bacterium of sporotrichum thermophile bacterium is evenly mixed according to the ratio of the weight ratio of 2:2:1:2:3, inoculate according to 5% mass ratio;

Turning: will inoculate good material and be the placement of heap shape, and when the temperature of heap body rises to 60 ℃, carry out the turning cooling, and ferment turning for the first time the 3rd day; 4th, 5,6,7 days every turning in 24 hours once, the 8th～12 day, every turning in 48 hours once, make heap temperature remain on 50 ℃;

Air flow: regulate heap body air flow by the mode of punching, at heap body temperature raising period and between the pliotherm period, temperature 〉=35 ℃ feed the high-pressure air of 0.4 MPa, and air flow is per 6 hours each 20min that ventilate; Between heap body cooldown period, temperature≤30 ℃ feed the high-pressure air of 0.4 MPa, and air flow is ventilation in per 24 hours 10 minutes;

Incubation time: 7 days.

(4) composting product drying, efflorescence, metering, packing

The dry temperature of heap soma is 40 ℃, is 10% to piling body water content, and windrow is pulverized, and crosses the 2mm sieve, and its living bacteria count is 2.3 hundred million/g, and cellulase activity is 300U/g, and the work of protein enzyme is 35 U/g, and moisture content is that 2%, pH is 5.5, fineness 70%; Utilize that packaging facilities measures, package, namely make commercial organic material composting agent.

Embodiment 2.

(1) strain improvement

(2) seed liquor preparation

B1, slant culture

B11, slant medium preparation

Koning trichoderma bacterium slant medium-grass meal 65g/L, ammonium sulfate 30g/L, potassium primary phosphate 5 g/L, sal epsom 1.5g/L, calcium chloride 0.15 g/L, regulating pH is 6.5, adds agar 15g/L, 60 ℃ of heat fused, pour disk into, the cutting slivering of cooling back is put into the eggplant bottle, sterilization;

Phanerochaete chrysosporium slant medium-wheat bran 130g/L boils the back with filtered through gauze, sucrose 12g/L, pH nature, adds agar 20g/L, sterilization;

Sporotrichum thermophile bacterium slant medium-peeling potato 200g/L is cut into small pieces and boils 16min, with four layers of filtered through gauze, with sucrose 20g/L, pH nature, adding agar 20g/L, sterilization;

B12, slant culture condition

Five kinds of thalline that above-mentioned test tube slant is cultivated, are inoculated in respectively in five kinds of slant mediums in the eggplant bottle respectively with after the dilution of 6ml sterilized water, and culture condition is specially:

The culture temperature of subtilis is 35 ℃, and incubation time is 30h;

The culture temperature of Bacillus licheniformis is 35 ℃, and incubation time is 30h;

The culture temperature of koning trichoderma bacterium is 27 ℃, and incubation time is 80h;

The culture temperature of Phanerochaete chrysosporium is 37 ℃, and incubation time is 84h;

The culture temperature of sporotrichum thermophile bacterium is 40 ℃, and incubation time is 84h;

B2, seed liquor preparation

B21, primary seed solution preparation: the bacterial classification aseptic water washing with slant culture in above-mentioned five eggplant bottles is prepared into five kinds of uniform bacteria suspensions; The viable count of regulating every kind of bacterium is 10,000,000,000 cfu/ml, and the viable count of fungi is 100,000,000 cfu/ml; Be stored in respectively in the aseptic Steel Vessel, standby;

B22, secondary seed solution preparation:

The preparation of subtilis secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 160 parts in wheat bran, 45 parts on husk, 35 parts of Semen Maydis powder, 25 parts of dregs of beans, 1.5 parts of sucrose, 0.8 part of potassium primary phosphate, 0. 9 parts of magnesium sulfate heptahydrates, 0.05 part of manganous sulfate, 4.5 parts of lime powders, 295 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned subtilis primary seed solution is inoculated shallow tray fermentation; Inoculate after 2 days, adopt colony counting method to measure the viable count in the tunning and regulate viable count with sterilized water and be the liquid seeds liquid of 10,000,000,000 cfu/ml, standby;

The preparation of Bacillus licheniformis secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 16 parts of dregs of beans, 45 parts of Semen Maydis powder, 220 parts in wheat bran, 4.5 parts of yeast powders, 0.8 part of potassium primary phosphate, 0.8 part of magnesium sulfate heptahydrate, 0.9 part of manganous sulfate, 14 parts in calcium carbonate, 5.5 parts of lime powders, 295 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned Bacillus licheniformis primary seed solution is inoculated shallow tray fermentation; Inoculate after 2 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 10,000,000,000 cfu/ml, standby;

The preparation of koning trichoderma bacterium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 110 parts on husk, 65 parts in wheat bran, 4.5 parts in ammonium sulfate, 1.5 parts of dipotassium hydrogen phosphates, 0.2 part of magnesium sulfate heptahydrate, 0.2 part of Calcium Chloride Powder Anhydrous, 210 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned koning trichoderma bacterium primary seed solution is inoculated shallow tray fermentation; Inoculate after 5 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 100,000,000 cfu/ml, standby;

The preparation of Phanerochaete chrysosporium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 230 parts of wood chips, 450 parts on husk, 150 parts in wheat bran, 15 parts in ammonium sulfate, 7 parts of dipotassium hydrogen phosphates, 0.6 part of magnesium sulfate heptahydrate, 0.7 part of Calcium Chloride Powder Anhydrous, 220 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned Phanerochaete chrysosporium primary seed solution is inoculated shallow tray fermentation; Inoculate after 5 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 100,000,000 cfu/ml, standby;

The preparation of sporotrichum thermophile bacterium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 160 parts on husk, 24 parts in wheat bran, 3.5 parts in ammonium sulfate, 1.5 parts of dipotassium hydrogen phosphates, 0.15 part of magnesium sulfate heptahydrate, 0.15 part of Calcium Chloride Powder Anhydrous, 220 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned sporotrichum thermophile bacterium primary seed solution is inoculated shallow tray fermentation; Inoculate after 5 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 100,000,000 cfu/ml, standby.

(3) pile fermentation

C1, pile fermentation substratum

Comprise following raw materials by weight percent: wood chip 31%, straw powder 18%, husk 22%, wheat bran 25%, sucrose 0.6%, ammonium sulfate 3%, dipotassium hydrogen phosphate 0.3%, magnesium sulfate heptahydrate 0.1%, natural pH value, water material weight ratio is 2:1;

C2, stack retting method and initial incubation condition

C3, initial fermentation condition is set

Water content: regulating initial water content is 55%, contains seed liquor;

Initial pH value: natural pH value;

Initial temperature: natural temperature;

Inoculum size: the secondary seed solution of subtilis, Bacillus licheniformis, koning trichoderma bacterium, Phanerochaete chrysosporium and five kinds of bacterium of sporotrichum thermophile bacterium is evenly mixed according to the ratio of the weight ratio of 2:2.5:1:2:2.5, inoculate according to 7% mass ratio;

Turning: will inoculate good material and be the placement of heap shape, and when the temperature of heap body rises to 65 ℃, carry out the turning cooling, and ferment turning for the first time the 3rd day; 4th, 5,6,7 days every turning in 24 hours once, the 8th～12 day, every turning in 54 hours once, make heap temperature remain on 30 ℃;

Air flow: regulate heap body air flow by the mode of punching, at heap body temperature raising period and between the pliotherm period, temperature 〉=35 ℃ feed the high-pressure air of 0.45 MPa, and air flow is per 8 hours each 16min that ventilate; Between heap body cooldown period, temperature≤30 ℃ feed the high-pressure air of 0.45MPa, and air flow is ventilation in per 30 hours 6 minutes;

Incubation time: 8 days.

(4) composting product drying, efflorescence, metering, packing

The dry temperature of heap soma is 45 ℃, is 8% to piling body water content, and windrow is pulverized, and crosses the 2mm sieve, is work in-process, and its living bacteria count is 8.1 hundred million/g, and cellulase activity is 450.8U/g, and the work of protein enzyme is 90.2 U/g, and moisture content is that 10.5%, pH is 6, fineness 80%; Utilize that packaging facilities measures, package, namely make commercial organic material composting agent.

Embodiment 3.

(1) strain improvement

(2) seed liquor preparation

B1, slant culture

B11, slant medium preparation

Koning trichoderma bacterium slant medium-grass meal 70g/L, ammonium sulfate 30 g/L, potassium primary phosphate 6 g/L, sal epsom 1.8g/L, calcium chloride 0.2g/L, regulating pH is 6.5, adds agar 15g/L, 85 ℃ of heat fused, pour disk into, the cutting slivering of cooling back is put into the eggplant bottle, sterilization;

Phanerochaete chrysosporium slant medium-wheat bran 130g/L boils the back with filtered through gauze, sucrose 13g/L, pH nature, adds agar 20g/L, sterilization;

Sporotrichum thermophile bacterium slant medium-peeling potato 200g/L is cut into small pieces and boils 18min, with four layers of filtered through gauze, with sucrose 20g/L, pH nature, adding agar 20g/L, sterilization;

B12, slant culture condition

Five kinds of thalline that above-mentioned test tube slant is cultivated, are inoculated in respectively in five kinds of slant mediums in the eggplant bottle respectively with after the dilution of 8ml sterilized water, and culture condition is specially:

The culture temperature of subtilis is 37 ℃, and incubation time is 36h;

The culture temperature of Bacillus licheniformis is 37 ℃, and incubation time is 36h;

The culture temperature of koning trichoderma bacterium is 28 ℃, and incubation time is 84h;

The culture temperature of Phanerochaete chrysosporium is 40 ℃, and incubation time is 80h;

The culture temperature of sporotrichum thermophile bacterium is 45 ℃, and incubation time is 80h;

B2, seed liquor preparation

B21, primary seed solution preparation: the bacterial classification aseptic water washing with slant culture in above-mentioned five eggplant bottles is prepared into five kinds of uniform bacteria suspensions; The viable count of regulating every kind of bacterium is 11,000,000,000 cfu/ml, and the viable count of fungi is 200,000,000 cfu/ml; Be stored in respectively in the aseptic Steel Vessel, standby;

B22, secondary seed solution preparation:

The preparation of subtilis secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 165 parts in wheat bran, 50 parts on husk, 40 parts of Semen Maydis powder, 25 parts of dregs of beans, 12 parts of sucrose, 1 part of potassium primary phosphate, 1 part of magnesium sulfate heptahydrate, 0.08 part of manganous sulfate, 5 parts of lime powders, 300 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned subtilis primary seed solution is inoculated shallow tray fermentation; Inoculate after 3 days, adopt colony counting method to measure the viable count in the tunning and regulate viable count with sterilized water and be the liquid seeds liquid of 11,000,000,000 cfu/ml, standby;

The preparation of Bacillus licheniformis secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 18 parts of dregs of beans, 50 parts of Semen Maydis powder, 230 parts in wheat bran, 5 parts of yeast powders, 1 part of potassium primary phosphate, 1.2 parts of magnesium sulfate heptahydrates, 0.12 part of manganous sulfate, 15 parts in calcium carbonate, 6 parts of lime powders, 300 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned Bacillus licheniformis primary seed solution is inoculated shallow tray fermentation; Inoculate after 3 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 11,000,000,000 cfu/ml, standby;

The preparation of koning trichoderma bacterium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 110 parts on husk, 70 parts in wheat bran, 5 parts in ammonium sulfate, 1.8 parts of dipotassium hydrogen phosphates, 0.4 part of magnesium sulfate heptahydrate, 0.35 part of Calcium Chloride Powder Anhydrous, 220 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned koning trichoderma bacterium primary seed solution is inoculated shallow tray fermentation; Inoculate after 6 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 200,000,000 cfu/ml, standby;

The preparation of Phanerochaete chrysosporium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 270 parts of wood chips, 500 parts on husk, 160 parts in wheat bran, 16 parts in ammonium sulfate, 8 parts of dipotassium hydrogen phosphates, 0.8 part of magnesium sulfate heptahydrate, 0.9 part of Calcium Chloride Powder Anhydrous, 240 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned Phanerochaete chrysosporium primary seed solution is inoculated shallow tray fermentation; Inoculate after 6 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 200,000,000 cfu/ml, standby;

The preparation of sporotrichum thermophile bacterium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 170 parts on husk, 30 parts in wheat bran, 4 parts in ammonium sulfate, 1.6 parts of dipotassium hydrogen phosphates, 0.17 part of magnesium sulfate heptahydrate, 0.2 part of Calcium Chloride Powder Anhydrous, 220 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned sporotrichum thermophile bacterium primary seed solution is inoculated shallow tray fermentation; Inoculate after 6 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 200,000,000 cfu/ml, standby.

(3) pile fermentation

C1, pile fermentation substratum

Comprise following raw materials by weight percent: wood chip 34%, straw powder 16%, husk 20%, wheat bran 24%, sucrose 0.8%, ammonium sulfate 4.5%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate heptahydrate 0.2%, natural pH value, water material weight ratio is 3:1;

C2, stack retting method and initial incubation condition

C3, initial fermentation condition is set

Water content: regulating initial water content is 60%, contains seed liquor;

Initial pH value: natural pH value;

Initial temperature: natural temperature;

Inoculum size: the secondary seed solution of subtilis, Bacillus licheniformis, koning trichoderma bacterium, Phanerochaete chrysosporium and five kinds of bacterium of sporotrichum thermophile bacterium is evenly mixed according to the ratio of the weight ratio of 2.5:2:1.5:2:2, inoculate according to 8% mass ratio;

Turning: will inoculate good material and be the placement of heap shape, and when the temperature of heap body rises to 70 ℃, carry out the turning cooling, and ferment turning for the first time the 3rd day; 4th, 5,6,7 days every turning in 24 hours once, the 8th～12 day, every turning in 60 hours once; Make heap temperature remain on 40 ℃;

Air flow: regulate heap body air flow by the mode of punching, at heap body temperature raising period and between the pliotherm period, temperature 〉=35 ℃ feed the high-pressure air of 0.5 MPa, and air flow is per 10 hours each 13min that ventilate; Between heap body cooldown period, temperature≤30 ℃ feed the high-pressure air of 0.5 MPa, and air flow is ventilation in per 36 hours 8 minutes;

Incubation time: 10 days.

(4) composting product drying, efflorescence, metering, packing

The dry temperature of heap soma is 50 ℃, is 7% to piling body water content, and windrow is pulverized, and crosses the 2mm sieve, and its living bacteria count is 13.8 hundred million/g, and cellulase activity is 805.1U/g, and the work of protein enzyme is 145.6 U/g, and moisture content is that 21.5%, pH is 7, fineness 70～100%; Utilize that packaging facilities measures, package, namely make commercial organic material composting agent.

Embodiment 4.

(1) strain improvement

(2) seed liquor preparation

B1, slant culture

B11, slant medium preparation

Koning trichoderma bacterium slant medium-grass meal 80g/L, ammonium sulfate 35 g/L, potassium primary phosphate 8 g/L, sal epsom 2 g/L, calcium chloride 0.3 g/L, regulating pH is 7, adds agar 15g/L, 100 ℃ of heat fused, pour disk into, the cutting slivering of cooling back is put into the eggplant bottle, sterilization;

Phanerochaete chrysosporium slant medium-wheat bran 140g/L boils the back with filtered through gauze, sucrose 15g/L, pH nature, adds agar 20g/L, sterilization;

Sporotrichum thermophile bacterium slant medium-peeling potato 200g/L is cut into small pieces and boils 20min, with four layers of filtered through gauze, with sucrose 20g/L, pH nature, adding agar 20g/L, sterilization;

B12, slant culture condition

Five kinds of thalline that above-mentioned test tube slant is cultivated, are inoculated in respectively in five kinds of slant mediums in the eggplant bottle respectively with after the dilution of 10ml sterilized water, and culture condition is specially:

The culture temperature of subtilis is 40 ℃, and incubation time is 24h;

The culture temperature of Bacillus licheniformis is 40 ℃, and incubation time is 24h;

The culture temperature of koning trichoderma bacterium is 32 ℃, and incubation time is 72h;

The culture temperature of Phanerochaete chrysosporium is 42 ℃, and incubation time is 72h;

The culture temperature of sporotrichum thermophile bacterium is 50 ℃, and incubation time is 72h;

B2, seed liquor preparation

B21, primary seed solution preparation: the bacterial classification aseptic water washing with slant culture in above-mentioned five eggplant bottles is prepared into five kinds of uniform bacteria suspensions; The viable count of regulating every kind of bacterium is 12,000,000,000 cfu/ml, and the viable count of fungi is 300,000,000 cfu/ml; Be stored in respectively in the aseptic Steel Vessel, standby;

B22, secondary seed solution preparation:

The preparation of subtilis secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 170 parts in wheat bran, 60 parts on husk, 50 parts of Semen Maydis powder, 30 parts of dregs of beans, 3 parts of sucrose, 1.5 parts of potassium primary phosphates, 1.5 parts of magnesium sulfate heptahydrates, 0.1 part of manganous sulfate, 6 parts of lime powders, 300 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned subtilis primary seed solution is inoculated shallow tray fermentation; Inoculate after 3 days, adopt colony counting method to measure the viable count in the tunning and regulate viable count with sterilized water and be the liquid seeds liquid of 12,000,000,000 cfu/ml, standby;

The preparation of Bacillus licheniformis secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 20 parts of dregs of beans, 55 parts of Semen Maydis powder, 240 parts in wheat bran, 6 parts of yeast powders, 1.5 parts of potassium primary phosphates, 1.5 parts of magnesium sulfate heptahydrates, 0.15 part of manganous sulfate, 16 parts in calcium carbonate, 6 parts of lime powders, 300 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned Bacillus licheniformis primary seed solution is inoculated shallow tray fermentation; Inoculate after 3 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 12,000,000,000 cfu/ml, standby;

The preparation of koning trichoderma bacterium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 120 parts on husk, 80 parts in wheat bran, 6 parts in ammonium sulfate, 2 parts of dipotassium hydrogen phosphates, 0.5 part of magnesium sulfate heptahydrate, 0.5 part of Calcium Chloride Powder Anhydrous, 230 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned koning trichoderma bacterium primary seed solution is inoculated shallow tray fermentation; Inoculate after 6 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 300,000,000 cfu/ml, standby;

The preparation of Phanerochaete chrysosporium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 300 parts of wood chips, 600 parts on husk, 200 parts in wheat bran, 20 parts in ammonium sulfate, 10 parts of dipotassium hydrogen phosphates, 1 part of magnesium sulfate heptahydrate, 1 part of Calcium Chloride Powder Anhydrous, 250 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned Phanerochaete chrysosporium primary seed solution is inoculated shallow tray fermentation; Inoculate after 6 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 300,000,000 cfu/ml, standby;

The preparation of sporotrichum thermophile bacterium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 180 parts on husk, 40 parts in wheat bran, 5 parts in ammonium sulfate, 2 parts of dipotassium hydrogen phosphates, 0.2 part of magnesium sulfate heptahydrate, 0.3 part of Calcium Chloride Powder Anhydrous, 240 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned sporotrichum thermophile bacterium primary seed solution is inoculated shallow tray fermentation; Inoculate after 6 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 300,000,000 cfu/ml, standby.

(3) pile fermentation

C1, pile fermentation substratum

Comprise following raw materials by weight percent: wood chip 35%, straw powder 12%, husk 24%, wheat bran 23%, sucrose 0.7%, ammonium sulfate 4.5%, dipotassium hydrogen phosphate 0.5%, magnesium sulfate heptahydrate 0.3%, natural pH value, water material weight ratio is 4:1;

C2, stack retting method and initial incubation condition

C3, initial fermentation condition is set

Water content: regulating initial water content is 60%, contains seed liquor;

Initial pH value: natural pH value;

Initial temperature: natural temperature;

Inoculum size: the secondary seed solution of subtilis, Bacillus licheniformis, koning trichoderma bacterium, Phanerochaete chrysosporium and five kinds of bacterium of sporotrichum thermophile bacterium is evenly mixed according to the ratio of the weight ratio of 2:2:2:2:2, inoculate according to 10% mass ratio;

Turning: will inoculate good material and be the placement of heap shape, and when the temperature of heap body rises to 80 ℃, carry out the turning cooling, and ferment turning for the first time the 3rd day; 4th, 5,6,7 days every turning in 24 hours once, the 8th～12 day, every turning in 72 hours once, make heap temperature remain on 45 ℃;

Air flow: regulate heap body air flow by the mode of punching, at heap body temperature raising period and between the pliotherm period, temperature 〉=35 ℃ feed the high-pressure air of 0.6 MPa, and air flow is per 12 hours each 10min that ventilate; Between heap body cooldown period, temperature≤30 ℃ feed the high-pressure air of 0.6 MPa, and air flow is ventilation in per 36 hours 5 minutes;

Incubation time: 12 days.

(4) composting product drying, efflorescence, metering, packing

The dry temperature of heap soma is 60 ℃, is 6% to piling body water content, and windrow is pulverized, and crosses the 2mm sieve, be work in-process, its living bacteria count is 22.8 hundred million/g, and cellulase activity is 950.8U/g, and the work of protein enzyme is 195.4 U/g, moisture content is that 30%, pH is 7.5, fineness 100%; Utilize that packaging facilities measures, package, namely make commercial organic material composting agent.

Application examples 1.

Protect to such an extent that board organic material composting agent plot experiment is summed up 1.1 rice straw is used

On November 30,10 days～2010 November in 2010 is in Xinfeng County, Shaoguan, Guangdong Province institute of agricultural sciences test base, (the organic material composting agent that present method makes is commercialization production and selling at present to organic material composting agent of the present invention, its product is by name protect the agent of board organic material composting, below relate to " protect " and all represent organic material composting agent of the present invention) the effect of becoming thoroughly decomposed measure.Wherein, soil property is silty loam, and stalk is excellent 368 rice straws of golden rice.Behind rice harvesting, Tian Ji is built in every residential quarter, and rice straw is tiled in the surface, residential quarter, is soaked with less water, and the omnidistance field that keeps of test is moistening.The colour-change appearance method of stalk, rotten degree is measured with tension method.Test arranges shown in following table 1-1:

Table 1-1 test arranges

Figure 2013102281715100002DEST_PATH_IMAGE018

1.2 result and analysis

Table 1-2 use protect the changing conditions of stalk after the agent of board organic material composting.

Figure 2013102281715100002DEST_PATH_IMAGE020

Annotate: it is remarkable that capitalization is illustrated in 0.01 level difference in the table, and lowercase is illustrated in 0.05 level difference significantly (DMRT method).

Important evaluating index during variation of stalk colour-change, the degree of becoming thoroughly decomposed is more high, and color is more dark.Along with the variation of test period, each value of thrust bacterium of handling descends gradually.By the table 1-2 find out, the colour-change degree varies of different treatment, wherein handle one aberration the most obvious, be changed to beige by initial bluish yellow look; Processing two is taken second place, and is changed to by initial bluish yellow look to be brown; Processing three belongs under the natural condition becomes thoroughly decomposed, and aberration changes not obvious.The variation of pulling force is consistent with change in color basically, and pulling force is more little, rotten degree is more high, is because in digest process, microbiological degradation in the stalk lignocellulose, reduced the compactness extent of stalk, cause the stalk pulling force to descend.1-2 finds out by table, and along with the variation of test period, each value of thrust bacterium of handling descends gradually.Test initial value pulling force is 6050g, and with the variation of the time of becoming thoroughly decomposed, the stalk value of thrust all has decline.In the time of the 20th day, the stalk pulling force has reduced 1080g and 2930g than processing two and control treatment respectively in the processing one, has utmost point significant difference.The result shows, uses and protects to such an extent that the agent of board organic material composting can be accelerated becoming thoroughly decomposed of rice straw.

1.3 conclusion: use and protect to such an extent that the agent of board organic material composting can be accelerated becoming thoroughly decomposed of rice straw, the 15th day left and right sides stalk after using reaches the effect of becoming thoroughly decomposed preferably.

Application examples 2

2.1 organic material composting agent screening varieties Test Summary in 2012

Carry in academy of agricultural sciences, Heilungkiang soil and fertilizer and resource environment institute frame on August 17,10 days～2012 May in 2012 and carry out, explore protect the agent of board organic material composting on the black earth of Harbin to the effect evaluation of becoming thoroughly decomposed of maize straw.The area of each frame is 2 * 2=4m ²

Test arranges two processing, and control treatment 1: maize straw does not add the agent of becoming thoroughly decomposed; Protect to such an extent that handle 2: the agent of becoming thoroughly decomposed of maize straw Jia Baode board.The maize straw that approaches of thickness and length is removed in choosing, is cut into about 2cm segment, according to the usage quantity of the agent 3kg/ mu Units of Account mesh bag area that becomes thoroughly decomposed.Each is parallel gets 40.00g oven dry stalk, the 0.38g agent of becoming thoroughly decomposed, and regulating water content is about 60%, and it is fully mixed.

Nylon Bag after on May 10th, 2012 will handle is imbedded 5～10cm soil layer.Each handles 21 weights

Multiple, sampling in the 10th day, 20 days, 30 days, 45 days, 60 days, 80 days 100 days, get three repetitions at random respectively at every turn.Adopt oven drying method to measure the stalk rate of weight loss.

2.2 interpretation of result

Table 2-1 stalk residual quantity in each in period

Figure 2013102281715100002DEST_PATH_IMAGE022

By table 2-1 as can be seen, maize straw becomes thoroughly decomposed along with its decomposition amount increase of extension of time.In the whole decomposition phase, present and decompose fast, the slow trend of later stage decomposition early stage.The maize straw initial stage, the stalk residual quantity of using organic material composting agent processing all was lower than control treatment to taking a sample latter stage.2-2 finds out by table, and straw-returning is during 10～30 days, and it is obvious to use the stalk decomposition efficiency of becoming thoroughly decomposed.Also the field is in the time of 20 days at corn, and the stalk rate of decomposition of using organic material composting agent processing is 26.1%, and there were significant differences on 1% and 5% level than control treatment.

Table 2-2 straw biological amount rate of decomposition variance table in each in period

Figure 2013102281715100002DEST_PATH_IMAGE024

Annotate: it is remarkable that capitalization is illustrated in 0.01 level difference, and lowercase is illustrated in 0.05 level difference significantly (DMRT method)

2.3 conclusion: use and protect to such an extent that the agent of board organic material composting can be accelerated maize straw and becomes thoroughly decomposed, decomposition efficiency is the highest in 20 days.

Application examples 3

3.1 application and the Test Summary thereof of organic material composting agent on paddy rice

On December 25,2 days～2012 September in 2012 in town, Wenchang, a Hainan Province garden Lam Tin Est. arrange verification experimental verification protect the become thoroughly decomposed effect and to the evaluation result of use of the fertilizer efficiency checking of following season paddy rice plantation of board organic material composting agent in rice straw direct returning to farmland pattern.

Test soil belongs to periodical water-logging type rice terrace, organic matter 2.15%, full nitrogen (%) 0.108, full phosphorus (%) 0.084, full potassium (%) 0.042, alkali-hydrolyzable nitrogen 38.5mg/kg, rapid available phosphorus 56.2mg/kg, available potassium 20.2mg/kg.The Ph value is 5.54.For examination organic material composting agent be Dongguan City protect the agent of board organic material composting, registration card number: microorganism fertilizer (2010) faces word (1525) number.

Test is set to three processing, and random alignment is handled in 30 square metres of every residential quarters, three repetitions.Test is handled as follows:

Control treatment 1: the conventional fertilizer of no straw-returning

Control treatment 2: conventional fertilizer+straw-returning (250kg/ mu)

Protect to such an extent that handle 3: conventional fertilizer+straw-returning (250kg/ mu)+protect the agent of board organic material composting

This season, preceding crop was early rice, and this season crop is for playing rice sky excellent 2168.(phosphate fertilizer 15kg/ mu, 16～16～16 homemade composite fertilizer 10kg/ mus applied fertilizer to the subsoil September 18.Cover rice straw in the residential quarter, spread the agent of becoming thoroughly decomposed (2kg/ mu), urea (5kg/ mu), plow.Tillering fertilizer (urea 6kg/ mu) was executed in rice transplanting on September 30 on October 10, executed ear manuer (16～16～16 homemade composite fertilizer 7.5kg) on November 2, gathered in the crops December 25, surveyed and produce.

3.2 interpretation of result

The table 3-1 stalk effect assessment of becoming thoroughly decomposed.

Figure 2013102281715100002DEST_PATH_IMAGE026

By table 3-1 as can be seen, control treatment 2 is become thoroughly decomposed slower, just begins after 7 days to change on form, and black yellow, soft, the careless stink of quality of stalk color does not become thoroughly decomposed yet after 15 days.Handle 3 than handle 2 become thoroughly decomposed very fast, after 2 days the stalk color begin thin out, become thoroughly decomposed substantially after 10 days, fully become thoroughly decomposed after 15 days.Under equal conditions, interpolation is protected to such an extent that the agent of board organic material composting was become thoroughly decomposed fast at least 5 days than the stalk that does not add the agent of becoming thoroughly decomposed.

Table 3-2 output and economic target analysis.

Figure 2013102281715100002DEST_PATH_IMAGE028

Annotate: paddy rice paddy is according to 3.0kg/ unit.It is remarkable that capitalization is illustrated in 0.01 level difference, and lowercase is illustrated in 0.05 level difference significantly (DMRT method).

By table 3-2 as can be seen, protect to such an extent that board organic material composting agent processing increases to 33kg and 9kg respectively than the mu mean yield that contrasts processing 1 and control treatment 2,99 yuan and 27 yuan of output value increases, it is extremely remarkable to handle differences.

3.3 conclusion

Under this experiment condition, protect to such an extent that the agent of board organic material composting has the tangible acceleration Decomposition of becoming thoroughly decomposed to straw directly returning to field, significantly increase rice yield and income.

Application examples 4

4.1 rice straw is used organic material composting agent field test report: carry out in Gu Long village, Ping Ji town, North Area, Qiezhou, Guangxi on November 1,22 days～2011 July in 2011 field test verify protect the effect of board organic material composting agent.It is 724 square metres that test area is set, and is husky mud field for examination soil, and previous crops is paddy rice.

Test arranges three processing, and each is handled three times and repeats, and the residential quarter area is 30.6 square metres, and district's group is arranged at random, and test design is as follows:

Control treatment one: conventional fertilizer application

Control treatment two: conventional fertilizer application+straw-returning (350kg/ mu)

Protect to such an extent that handle three: conventional fertilizer application+straw-returning (350kg/ mu)+2kg/ mu protect the agent of board organic material composting.

Test on July 22nd, 2011 litter wholely, bulk application and test handle, rice transplanting on July 23 in 2011, results were surveyed and were produced on November 1st, 2011, singles claim wet paddy folding to dry rate.The stalk that records each period becomes thoroughly decomposed and the changing conditions of rice biological amount.

4.2 interpretation of result

The character description of table 4-1 stalk.

Figure 2013102281715100002DEST_PATH_IMAGE030

Annotate: protect to such an extent that handle three and began to occur emitting the bubble phenomenon at the 3rd day, control treatment two began to emit bubble at the 5th day, by table 4-1 as can be seen, protect to such an extent that handle three and handle than contrast that second colors are pitch black, quality is softer, rancid smell is stronger, the time of rotting shortens 3～4 days.

Table 4-2 different treatment is to the economical character of paddy rice

Figure 2013102281715100002DEST_PATH_IMAGE032

By table 4-2 as can be seen, protect to such an extent that handle three thousand seed weight and respectively control treatment one and control treatment two have been increased 0.2g and 0.3g, plant height increase 0.2cm and～0.3cm.

Each handles examination output statistics table table 4-3

Figure 2013102281715100002DEST_PATH_IMAGE033

Output is carried out variance analysis, and the result is shown in table 4-4:

Table 4-4 analysis of variance table.

Figure 2013102281715100002DEST_PATH_IMAGE035

4-4 draws by table, and the check of F value is handled differences and reached conspicuous level (F=6.61 is less than F0.05).

Table 4-5 output and output value comparison sheet

Figure 2013102281715100002DEST_PATH_IMAGE037

Annotate: the paddy rice valency is 2.4 yuan/kg, the agent of becoming thoroughly decomposed is by 20 yuan of/mu calculating.

By table 4-5 as can be seen, use protect the board organic material composting agent effect that can reach volume increase, increase income, per mu yield is 485.1kg, mu volume increase 14.5kg, stimulation ratio is 3.1%, 14.80 yuan of mu net return.

4.3 conclusion: use and protect to such an extent that the agent of board organic material composting can be done sth. in advance 3～4 days rice straws that become thoroughly decomposed, certain effect that increases both production and value is arranged.

Application examples 5

5.1 organic material composting agent in 2012 screening field test report

On November 2nd, 2012 arranged test in the Zhongjiang County Cang Shan of Guanghan City, Sichuan Province town, become thoroughly decomposed effect and effect of increasing production to 17 kinds of organic material composting agent on the market are screened, numbering is respectively No. 1～No. 17, wherein protects to such an extent that the agent of board organic material composting is No. 17 specimen.

3 processing are established in the rape test, and each is handled three times and repeats, and completely random is arranged, and the residential quarter area is 32 square metres.Rape adopts culturing and transplanting seedlings, and transplanting density is 6000 strain/mus, bulk application.Carried out straw-returning and broadcasted sowing the agent of becoming thoroughly decomposed in the 8th day after rape survives, and guaranteed stalk weight basically identical, water content is 50～70%.The identical measure of each repetition guarantees to finish on the same day, and the residential quarter design content is as follows:

Handle one: contrast arranges, conventional fertilizer application, no straw-returning

Handle two: the agent straw-returning that do not become thoroughly decomposed, conventional fertilizer application+straw-returning

Handle three: conventional fertilizer application+straw-returning+No. 17 organic material composting agent

Before and after the growth of rape phase, multidraw is measured the degree record of becoming thoroughly decomposed of soil physical and chemical property, stalk and is surveyed with mensuration, rape and produce and the economic characters description.

5.2 interpretation of result

(1) change of soil fertility

Table 5-1 different treatment is to the influence of soil physico-chemical property

Figure 2013102281715100002DEST_PATH_IMAGE038

By table 5-1 as can be seen, after straw-returning was carried out in the rice field, the soil weight and pH value descended to some extent, and nutrient contents such as the soil organism, full nitrogen, full phosphorus, rapid available phosphorus, full potassium, available potassium all increase to some extent.Handle two and handle an organic average 0.89g/kg of increasing, full nitrogen increases by 1.13 g/kg.Use No. 17 organic material composting agent on the straw-returning basis.Organic matter handles one and handle two branches and increase by 2.22 g/kg and 1.33 g/kg, and amplification is 6.68% and 3.90%, and full nitrogen handles one and handle two branches and increase by 0.20 g/kg and 0.05 g/kg.Amplification is 10.00% and 2.33%.The result shows, borrows on the individual also basis, field, uses No. 17 organic material composting agent, can strengthen the soil fertilizer water retention capacity, and it is impolite to improve soil, strengthens microbial activity.

(2) to the become thoroughly decomposed influence of speed and degree of stalk: table 5-2 respectively handles stalk pulling force relatively (N)

Figure 2013102281715100002DEST_PATH_IMAGE040

5-2 finds out that along with the decomposition of rice straw, the rice straw value of thrust descends gradually by table.Handle two and do not execute when becoming thoroughly decomposed agent, the value of thrust reduction amplitude of rice straw is 31.99% during the rape results.Contrast two, processing three can be accelerated the decomposition of rice straw, in the rape results is, its value of thrust is 43.34, is lower than control treatment 2 17.84, reduces; Value of thrust is 38.86.The field observation result is consistent in this result, and its color is darker, smell rots more and have tangible mycelia to occur.Illustrating to use protects for No. 17 to such an extent that the agent of board organic material composting can be accelerated rice straw and becomes thoroughly decomposed.

(3) biological character

The brassinosteroid biosynthesis amount of table 5-3 different treatment time

Figure 2013102281715100002DEST_PATH_IMAGE042

Annotate: multiple comparisons adopts the Duncan method, and different alphabets are variant each other in the table.Down together.

5-3 finds out that along with the extension in treatment time, each handles biomass increases gradually by table, and in the different treatment stage, brassinosteroid biosynthesis amount bacterium showed as and handles three between each was handled〉handle two〉handle one.When gathering in the crops to rape, handle two and handle one and increase 49.00g, amplification 33.04% is handled and three is handled two, handles one and increase by 43.93% and 91.48% respectively, can significantly increase the brassinosteroid biosynthesis amount.

(4) to the influence of rape economical character and output thereof.

Table 5-4 different treatment is to the performance of rape economical character and output thereof

Figure 2013102281715100002DEST_PATH_IMAGE044

Annotate: lowercase alphabet is shown in 0.05 level difference significantly (DMRT method) in the table

By table 5-4 as can be seen, than control treatment one, processing two and processing three all can increase yield of rape to a certain extent.Only under the straw-returning condition, handle one and can improve several 5.68%, the thousand seed weight 1.33% of individual plant effective angle fruit sum 3.61%, every angle pod grain, there was no significant difference between pod grain number and the thousand seed weight, output handles one increases by 3.81%.Under the straw-returning condition, use No. 17 agent of becoming thoroughly decomposed, handle one and can improve several 6.44%, the thousand seed weight 2.40% of individual plant effective angle fruit sum 8.05%, every angle pod grain, have significant difference between pod grain number and the thousand seed weight.Handle two, increase by 4.29%, 0.72%, 1.05% respectively, there is significant difference in each between handling.Handling under three conditions, rape per mu yield 182.81kg handles two and handle one and increase production 6.63% and 10.69 respectively, obvious effect of increasing production.

5.3 conclusion: under the weather condition of Zhongjiang County, when other farming activities such as Fertilization Level are identical, use and protect to such an extent that the agent of board organic material composting can be accelerated becoming thoroughly decomposed of stalk, improve stalk decomposition rate, strengthen soil fertility level, the kind of rape is implanted with the increasing both production and income effect.

The present invention is described by preparation method and the field effect to the organic material composting agent, inquired into preparation technology and the effect of the agent of this patent organic material composting, drawn a kind of concise production process, perfect, energy consumption is low, the preparation method of free of contamination organic material composting agent.

On this technology basis, prepared a kind of decomposition time that can significantly shorten stalk, increase soil fertility, the safety of increasing both production and income, organic material composting agent efficiently, be the also important behave in the pattern of field of China's agricultural stalk, for China's agricultural sustainable development is made important exploration.

Should be noted that at last; above embodiment is only in order to illustrate technical scheme of the present invention; but not limiting the scope of the invention; although with reference to preferred embodiment the present invention has been done to explain; those of ordinary skill in the art is to be understood that; can make amendment or be equal to replacement technical scheme of the present invention, and not break away from essence and the scope of technical solution of the present invention.

Claims

1. the preparation method of commercialization organic material composting agent is characterized in that it may further comprise the steps:

(1) strain improvement

A2, strain bio security are identified: according to microbial fertilizer Biosafety current techique above-mentioned five kinds of bacterial classifications are carried out security and identify, remove poisonous unsafe bacterial strain;

(2) seed liquor preparation

B1, slant culture

B11, slant medium preparation

Phanerochaete chrysosporium slant medium-wheat bran 120～140g/L boils the back with filtered through gauze, sucrose 10～15g/L, pH nature, adds agar 20g/L, sterilization;

Sporotrichum thermophile bacterium slant medium-peeling potato 200g/L is cut into small pieces and boils 15～20min, with four layers of filtered through gauze, with sucrose 20g/L, pH nature, adding agar 20g/L, sterilization;

B12, slant culture condition

After five kinds of thalline that above-mentioned test tube slant is cultivated dilute with 5～10ml sterilized water respectively, in the eggplant bottle, be inoculated in respectively in five kinds of slant mediums, culture condition is: culture temperature is 25～50 ℃, and incubation time is 24～96h;

B2, seed liquor preparation

B21, primary seed solution preparation: the bacterial classification aseptic water washing with slant culture in above-mentioned five eggplant bottles is prepared into five kinds of uniform bacteria suspensions; The viable count of regulating every kind of bacterium is 80～12,000,000,000 cfu/ml, and the viable count of fungi is 0.5～300,000,000 cfu/ml; Be stored in respectively in the aseptic Steel Vessel, standby;

B22, secondary seed solution preparation:

The preparation of subtilis secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 150～170 parts in wheat bran, 40～60 parts on husk, 30～50 parts of Semen Maydis powder, 20～30 parts of dregs of beans, 1～3 part of sucrose, 0.5～1.5 part of potassium primary phosphate, 0. 5～1.5 parts of magnesium sulfate heptahydrates, 0.01～0.1 part of manganous sulfate, 4～6 parts of lime powders, 290～300 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned subtilis primary seed solution is inoculated shallow tray fermentation; Inoculate after 2～3 days, adopt colony counting method to measure the viable count in the tunning and regulate viable count with sterilized water and be the liquid seeds liquid of 80～12,000,000,000 cfu/ml, standby;

The preparation of Bacillus licheniformis secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 15～20 parts of dregs of beans, 40～55 parts of Semen Maydis powder, 200～240 parts in wheat bran, 4～6 parts of yeast powders, 0.5～1.5 part of potassium primary phosphate, 0.5～1.5 part of magnesium sulfate heptahydrate, 0.05～0.15 part of manganous sulfate, 12～16 parts in calcium carbonate, 5～6 parts of lime powders, 295～300 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned Bacillus licheniformis primary seed solution is inoculated shallow tray fermentation; Inoculate after 2～3 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 80～12,000,000,000 cfu/ml, standby;

The preparation of koning trichoderma bacterium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 100～120 parts on husk, 60～80 parts in wheat bran, 4～6 parts in ammonium sulfate, 1～2 part of dipotassium hydrogen phosphate, 0.1～0.5 part of magnesium sulfate heptahydrate, 0.1～0.5 part of Calcium Chloride Powder Anhydrous, 200～230 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned koning trichoderma bacterium primary seed solution is inoculated shallow tray fermentation; Inoculate after 5～6 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 0.5～300,000,000 cfu/ml, standby;

The preparation of Phanerochaete chrysosporium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 200～300 parts of wood chips, 400～600 parts on husk, 100～200 parts in wheat bran, 10～20 parts in ammonium sulfate, 5～10 parts of dipotassium hydrogen phosphates, 0.5～1 part of magnesium sulfate heptahydrate, 0.5～1 part of Calcium Chloride Powder Anhydrous, 200～250 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned Phanerochaete chrysosporium primary seed solution is inoculated shallow tray fermentation; Inoculate after 5～6 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 05～300,000,000 cfu/ml, standby;

The preparation of sporotrichum thermophile bacterium secondary seed solution: the solid fermentation substratum comprises the raw material of following weight part: 150～180 parts on husk, 20～40 parts in wheat bran, 3～5 parts in ammonium sulfate, 1～2 part of dipotassium hydrogen phosphate, 0.1～0.2 part of magnesium sulfate heptahydrate, 0.1～0.3 part of Calcium Chloride Powder Anhydrous, 200～240 parts in water; Sterilize after the packing, sterilized 120 minutes for 121 ℃, be cooled to normal temperature, above-mentioned sporotrichum thermophile bacterium primary seed solution is inoculated shallow tray fermentation; Inoculate after 5～6 days, measure viable count in the tunning, regulate viable count with sterilized water and be the liquid seeds liquid of 0.5～300,000,000 cfu/ml, standby;

(3) pile fermentation

C1, pile fermentation substratum

Comprise following raw materials by weight percent: wood chip 30～35%, straw powder 10～20%, husk 15～25%, wheat bran 20～25%, sucrose 0.5～1%, ammonium sulfate 3～5%, dipotassium hydrogen phosphate 0.3～0.5%, magnesium sulfate heptahydrate 0.1～0.3%, nature pH value, water material weight ratio is 1:1～4:1;

C2, stack retting method and initial incubation condition

C3, initial fermentation condition is set

Initial pH value: natural pH value;

Initial temperature: natural temperature;

Incubation time: 7～12 days;

(4) composting product drying, efflorescence, metering, packing

2. the preparation method of a kind of commercialization organic material composting according to claim 1 agent, it is characterized in that: described step B12, culture condition are specially:

3. the preparation method of a kind of commercialization organic material composting according to claim 1 agent, it is characterized in that: in the first order seed liquid preparing process of described step B21, the viable count of every kind of bacterium is 90～11,500,000,000 cfu/ml, and the viable count of fungi is 1～200,000,000 cfu/ml.

4. the preparation method of a kind of commercialization organic material composting according to claim 1 agent is characterized in that: the solid fermentation substratum of the subtilis in the secondary seed solution preparation of described step B22 comprises the raw material of following weight part: 155～165 parts in wheat bran, 45～55 parts on husk, 35～45 parts of Semen Maydis powder, 25～28 parts of dregs of beans, 1.5～2 parts of sucrose, 0.5～1 part of potassium primary phosphate, 0. 5～1 part of magnesium sulfate heptahydrate, 0.03～0.08 part of manganous sulfate, 4.5～5 parts of lime powders, 298 parts in water.

5. the preparation method of a kind of commercialization organic material composting according to claim 1 agent is characterized in that: the solid fermentation substratum of the Bacillus licheniformis in the secondary seed solution preparation of described step B22 comprises the raw material of following weight part: 16～18 parts of dregs of beans, 45～50 parts of Semen Maydis powder, 200～220 parts in wheat bran, 4.5～5.5 parts of yeast powders, 0.5～1 part of potassium primary phosphate, 0.5～1.5 part of magnesium sulfate heptahydrate, 0.05～0.1 part of manganous sulfate, 12～14 parts in calcium carbonate, 5.5 parts of lime powders, 300 parts in water.

6. the preparation method of a kind of commercialization organic material composting according to claim 1 agent, it is characterized in that: the solid fermentation substratum of the koning trichoderma bacterium in the secondary seed solution preparation of described step B22 comprises the raw material of following weight part: 105～115 parts on husk, 65～70 parts in wheat bran, 4～5 parts in ammonium sulfate, 1～1.5 part of dipotassium hydrogen phosphate, 0.1～0.5 part of magnesium sulfate heptahydrate, 0.1～0.5 part of Calcium Chloride Powder Anhydrous, 220 parts in water.

7. the preparation method of a kind of commercialization organic material composting according to claim 1 agent is characterized in that: the solid fermentation substratum of the Phanerochaete chrysosporium in the secondary seed solution preparation of described step B22 comprises the raw material of following weight part: 240～280 parts of wood chips, 440～500 parts on husk, 80～160 parts in wheat bran, 13～17 parts in ammonium sulfate, 5～8 parts of dipotassium hydrogen phosphates, 0.5～0.8 part of magnesium sulfate heptahydrate, 0.6～1 part of Calcium Chloride Powder Anhydrous, 200～210 parts in water.

8. the preparation method of a kind of commercialization organic material composting according to claim 1 agent is characterized in that: the solid fermentation substratum of the sporotrichum thermophile bacterium in the secondary seed solution preparation of described step B22 comprises the raw material of following weight part: 160～170 parts on husk, 25～38 parts in wheat bran, 3～4 parts in ammonium sulfate, 1～1.5 part of dipotassium hydrogen phosphate, 0.1～0.2 part of magnesium sulfate heptahydrate, 0.1～0.3 part of Calcium Chloride Powder Anhydrous, 220 parts in water.

9. the preparation method of a kind of commercialization organic material composting according to claim 1 agent, it is characterized in that: the pile fermentation substratum comprises following raw materials by weight percent among the described C1: wood chip 33～35%, straw powder 12～15%, husk 20～25%, wheat bran 22～24%, sucrose 0.5～1%, ammonium sulfate 3～5%, dipotassium hydrogen phosphate 0.3～0.5%, magnesium sulfate heptahydrate 0.1～0.3%, natural Ph value, water material weight ratio are 1:1～2:1.

10. the preparation method of a kind of commercialization organic material composting according to claim 1 agent, it is characterized in that: in the inoculating process of step C3, the ratio of the weight ratio of the secondary seed solution of subtilis, Bacillus licheniformis, koning trichoderma bacterium, Phanerochaete chrysosporium and five kinds of bacterium of sporotrichum thermophile bacterium is 2:2.5:1:2.5:2, and secondary seed solution and pile fermentation substratum are inoculated with 6% mass ratio.