CN103235092A - Testing device for indoor toxicity of insecticide to shoot-species piercing-sucking pests - Google Patents

Abstract

The invention relates to a device for measuring the indoor toxicity of insecticides to branch-sucking type pests, which comprises a bioassay cover, a bottom cover and a net cover of the bioassay cover which are penetrated at both ends, and is characterized in that: the bioassay cover The lower part is connected with a bottom cover, the upper part of the biometric cover is connected with a mesh cover of the biometric cover, and a cylindrical container is fixed on the middle part of the bottom cover in the biometric cover, and a cylindrical container is filled with a Absorbent columns that moisturize branch tips. The device of the present invention is simple to make, easy to use, and has a wide range of applications. It is not only suitable for indoor toxicity determination of insecticides on piercing-sucking pests, but also can be used for long-distance carrying of piercing-sucking live insects and ensures the integrity of insects. and survival rates.

Description

An indoor toxicity test device for insecticides against branch-sucking pests

technical field

The invention relates to a new device for indoor toxicity determination of insecticides on branch-sucking pests, which is applied to the indoor biological activity measurement of pesticides, and can also be used for long-distance carrying of living insects.               

Background technique

In the process of developing new pesticides, it is often necessary to measure the biological activity of pesticides on pests, and in the process of scientific research related to the field of pesticide research, such as monitoring of pest resistance to pesticides, all involve the determination of pesticide toxicity indoors. The current "People's Republic of China Agricultural Industry Standards" The determination methods stipulated in the indoor bioassay test guidelines for pesticides (insecticides) are drip method, poisonous leaf method, fumigation method, leaf soaking method, and insect soaking method, among which the drip method is mainly used for pests with piercing-sucking mouthparts However, these guidelines only stipulate the specifications for the treatment of experimental insects, experimental plant tissue materials and experimental chemicals, and how to ensure the nutrient supply of isolated plant tissue materials after the experimental treatment to ensure that the experimental There are no clear regulations on the normal feeding of insects, especially in the indoor toxicity test of insecticides on branch-sucking pests, the moisture retention of in vitro experimental plant tissue materials, nutrient supply and the provision of suitable test space for experimental insect activities. The environment is the key link for the success of the experiment and obtaining accurate data. At present, in the process of scientific research experiments on the determination of the toxicity of pesticides, the experimental plant tissue materials are treated with water-absorbing materials such as filter paper, absorbent cotton, and flower mud to implement simple moisturizing measures, and then placed in plastic bottles or petri dishes to treat the experimental insects. Carry out simple feeding and observation, but at present these methods all have obvious defects: firstly, the moisture-retaining material is not tightly and immovably combined with the test container, and the moisture-retaining material is often caused to move during the experimental observation and the test container is moved, causing the plant tissue material to fall from the moisture-retaining material. If they fall off, it is easy to cause the experimental insects to be squeezed and die; moreover, the nutrients and water absorbed by these moisturizing materials are limited, which can only play a short-term role, and cannot continue to meet the moisturizing and nutrient supply of isolated experimental plant tissue materials for a long time It is often easy to cause the lack of nutrition and water in the branch tissue of the isolated plant, resulting in the death of the plant tissue, and the death of the experimental insect due to lack of food; in addition, the currently used test container cannot provide a ventilated and unobstructed activity space environment for the experimental insect. The humidity in the test container is too high and the oxygen is lacking, leading to the phenomenon of suffocation and death of a large number of experimental insects; resulting in inaccurate results of the indoor toxicity test of insecticides and even the failure of the experiment, wasting a lot of manpower, material and financial resources. In addition, There is also the use of agar layer as a nutrient source and moisture-retaining material for short-term cultivation of detached plant leaves, but the operation process of this method is cumbersome, and it cannot truly simulate the actual state of pests' field activities and feeding. The above-mentioned problems are particularly obvious in the indoor virulence determination experiment of branch-like piercing-sucking insects. Measure research work. In addition, in the process of pest resistance monitoring and research, it is necessary to collect pests from different areas for indoor virulence determination, which involves the problem of long-distance carrying of experimental insects, and if the experimental insects lack food and ventilation during long-distance carrying It will cause a decline in their activity or a large number of deaths, which will inevitably cause the experimental insects carried over long distances to fail to meet the requirements of indoor experiments, resulting in the failure of normal experiments or inaccurate experimental results. Therefore, it is the research purpose of the present invention to address the above problems.

Contents of the invention

The object of the present invention is to provide a kind of device for measuring the indoor toxicity of insecticides to branch-like piercing-sucking pests, which helps to solve the problem that existing experimental insects are easy to measure the indoor toxicity of insecticides to branch-like piercing-sucking pests. Extruded, isolated plant branches lack nutrients and water supply, leading to dryness, dehydration or even death, and experimental insects lack a ventilated and unobstructed activity space environment, resulting in inaccurate experimental results or experimental failure. The invention of this device solves the problem of In the determination of the indoor toxicity of insecticides, the experimental insects are easy to be squeezed, the branch tissue of the isolated plants cannot be effectively fixed and the continuous and stable supply of nutrients and water, and the space environmental conditions for the experimental insects to provide ventilation and unobstructed activities are more realistic. The actual state of field activity and feeding of pests is simulated, and the accuracy and reliability of indoor toxicity determination results of insecticides on branch-sucking pests are improved.

Technical scheme of the present invention is:

An indoor toxicity test device for insecticides on branch-sucking type pests, comprising a bioassay cover, a bottom cover and a bioassay cover net cover through which both ends are penetrated, and is characterized in that: the lower part of the bioassay cover is connected with Bottom cover, the upper part of the biometric cover is connected with the mesh cover of the biometric cover, and the middle part of the bottom cover in the biometric cover is fixed with a cylinder container, and the cylinder container is filled with a twig tip for fixing and moisturizing. water-absorbing columns.

Wherein, a gauze window is opened on the side wall of the biometric cover.

A gauze window is opened in the middle of the net cover of the biometric cover.

The water-absorbing columnar body includes an upper half and a lower half, the outer side of the upper half is closely attached to the inner side of the cylindrical container, and the outer side of the lower half is 0.5-1.0 cm away from the inner side of the cylindrical container, forming The space gap used to hold the nutrient solution.

The water-absorbing columnar body is a solid water-absorbing material of sponge or flower mud.

The upper part of the biometric cover and the net cover of the biometric cover and the lower part of the biometric cover and the bottom cover are all sealed socket connections.

The bottom cover, the mesh cover of the biometric cover, the biometric cover, and the cylindrical container are made of transparent plastic materials.

The advantages of the present invention are:

① The new device is low in cost, easy to manufacture and convenient to use;

②The small cylindrical container on the bottom cover of the new device is used as a nutrient solution container to provide nutrients and water sources for the branch tips of the isolated plants in the experiment, to ensure the freshness of the branch tips for a long time, and to reduce the lack of nutrients caused by the experimental insects. causing death;

③The small cylindrical container on the bottom cover of the new device is embedded with solid water-absorbing materials such as cylindrical sponge or flower mud, which can be used as the fixation and moisturizing effect of the isolated plant branch tissue and branch tip, which solves the current problem of indoor toxicity. During the force measurement process, the plant tissue material falling off due to the movement of the test container and the movement of the moisturizing material caused the experimental insects to be squeezed to death, which also more realistically simulated the actual state of pests' field activities and feeding;

④ The side of the cylinder of the bioassay cover of the new device is equipped with a gauze window, which is conducive to ventilation and ventilation, ensuring that the test environment where the experimental insects live has stable air humidity and oxygen, and provides a barrier-free ventilation for the experimental insects. The activity space environment reduces the death of experimental insects due to suffocation;

⑤ The mesh cover of the bioassay hood and the bioassay hood of the new device are connected by a sealed socket, which facilitates the introduction of experimental insects into the bioassay hood and observation of the experimental process, and adds a ventilated window for the experimental insects;

⑥ The new device is suitable for indoor toxicity determination of insecticides on branch-sucking insects, and can also be used for long-distance carrying of piercing-sucking insects to ensure the integrity and survival rate of insects.

Description of drawings

Fig. 1 is a schematic diagram of the overall appearance and structure of an embodiment of the present invention.

Fig. 2 is a schematic diagram of the cross-sectional structure along the line A-A of Fig. 1 .

Detailed ways

In order to make the above-mentioned features and advantages of the present invention more comprehensible, the following specific embodiments are described in detail with reference to the accompanying drawings.

With reference to Fig. 1 and Fig. 2, the present invention relates to a kind of indoor virulence determination device of insecticides to branch-like piercing-sucking pests, comprising a bioassay cover 1, a bottom cover 2 and a bioassay cover mesh cover 3 penetrating through both ends, The lower part of the biometric cover 1 is connected with a bottom cover 2, the upper part of the biometric cover 1 is connected with a mesh cover 3 of the biometric cover, and a cylinder container 4 is fixed on the middle part of the bottom cover in the biometric cover 1, The columnar container 4 is filled with a water-absorbing columnar body 5 for fixing and moisturizing the shoots 7 .

A gauze window 1-1 is opened on the side wall of the above-mentioned biometric cover 1 .

A gauze window 3-1 is provided at the middle part of the above-mentioned biometric cover mesh cover 3 .

The above-mentioned water-absorbing columnar body 5 includes an upper half and a lower half, the outer side of the upper half is closely attached to the inner side of the cylindrical container, and the outer side of the lower half is 0.5-1.0 cm away from the inner side of the cylindrical container, forming Space gap for containing nutrient solution 6 .

The above-mentioned water-absorbing columnar body 5 is a solid water-absorbing material of sponge or flower mud.

The upper part of the biometric cover 1 and the net cover 3 of the biometric cover 3 and the lower part of the biometric cover 1 and the bottom cover 2 are sealed socket connections.

The bottom cover 2, the biometric cover net cover 3, the biometric cover 1, and the cylindrical container 4 are made of transparent plastic materials.

The biometric cover 1 of the new device is a cylindrical body or a square container made of transparent plastic material, and the volume is preferably 500-1000 cm ³ , and the volume of the main body of the new device can be adjusted according to the size of the actual test sample. The internal body space formed by the cover 2, the bioassay cover 1 and the bioassay cover net cover 3 is used as an active and feeding space environment for the insects in the virulence measurement experiment.

In order to facilitate the nutrient solution to hold and place the water-absorbing columns of plant shoots, the column container 4 is a non-detachable upright and fixedly installed on the inner side of the bottom cover 2 in the middle.

In order to ensure that the branch tips can be effectively fixed on the moisture-retaining material during the experiment and to avoid the phenomenon that the experimental insects are squeezed due to the movement of the moisture-retention material, the water-absorbing columnar body 5 of the new device is a column-shaped solid water-absorbing material. The whole is inseparable and equally divided into the upper half and the lower half. The upper end of the upper half of the cylindrical water-absorbing column is flush with the open end of the cylindrical container 4, and the lower end of the lower half is flush with the bottom of the cylindrical container 4. The ends are flush, the outer side of the upper half of the water-absorbing column is closely attached to the inner side of the cylindrical container 4 on the bottom cover, and the outer side of the lower half is 0.5-1.0 cm away from the inner side of the cylindrical container 4, as a nutrient solution The space provides continuous nutrition and water supply for the water-absorbing columnar body 5 .

In order to facilitate the experimental operation and the cleaning and repeated use of the device, the inner edge of the bottom cover 2 of the new device and the outer edge of the bottom end of the biometric cover 1 are in a sealed socket connection.

In order to ensure that the experimental insects are in suitable humidity and air-permeable environmental conditions, three gauze windows 1-1 are provided on the side of the bioassay cover 1 of the new device.

In order to introduce experimental insects into the biometric cover 1, and to prevent the experimental insects from escaping and observing the experimental process during the experiment, the biometric cover net cover 3 of the new device is connected to the top opening of the biometric cover 1 by a sealed socket connection. The central part of the biometric cover net cover 3 is a screen window 3-1, which also increases a ventilation window for experimental insects.

During the experiment, a certain amount of nutrient aqueous solution was put into the column container on the bottom cover of the new device, and the water-absorbing column was soaked with the nutrient solution and embedded in the column container on the bottom cover, and treated with insecticide. Insert the branch tip of the isolated plant into the water-absorbing columnar body, then insert the bioassay cover into the bottom cover, make the bioassay cover and the bottom cover tightly connected, introduce the experimental insects from the upper opening of the bioassay cover, and then insert the bioassay cover net The cover was inserted into the upper end of the bioassay cover to make it tightly connected, and finally the whole new device was moved into an artificial climate chamber that met the temperature and humidity conditions of the experimental design, and the number of dead insects in the experiment was regularly observed and recorded according to the experimental design requirements.

The above descriptions are only preferred embodiments of the present invention, and all equivalent changes and modifications made according to the scope of the patent application of the present invention shall fall within the scope of the present invention.

Claims (7)

1. a pesticide is to branch class sucking pest Toxicity Determination device, comprise living cover, bottom and the living cover square of surveying surveyed that two ends are run through, its feature exists: the described bottom of surveying cover of giving birth to is connected with bottom, the described top of surveying cover of giving birth to is connected with the living cover square of surveying, be positioned at living the survey on the bottom middle part of covering and be installed with column container, be filled with a water absorptivity column in order to fixing and the branch of preserving moisture in the described column container.

2. a kind of pesticide according to claim 1 is characterized in that branch class sucking pest Toxicity Determination device: described giving birth on the sidewall of surveying cover offers the gauze window.

3. a kind of pesticide according to claim 1 and 2 is characterized in that branch class sucking pest Toxicity Determination device: the described middle part of surveying the cover square of giving birth to offers the gauze window.

4. a kind of pesticide according to claim 3 is to branch class sucking pest Toxicity Determination device, it is characterized in that: described water absorptivity column comprises the first half and Lower Half, closely paste mutually with the column container inboard in the outside of the described first half, the outside of described Lower Half and column container inner distance 0.5 ~ 1.0cm form the spatial joint clearance in order to the splendid attire nutrient solution.

According to claim 1 or 4 described a kind of pesticides to branch class sucking pest Toxicity Determination device, it is characterized in that: described water absorptivity column is the decorating film water-absorbing material of sponge or flower mud.

6. a kind of pesticide according to claim 5 is characterized in that branch class sucking pest Toxicity Determination device: described top that give birth to survey cover is the enclosure-type socket joint between the bottom of covering and the bottom and is connected with giving birth to survey between the cover square and give birth to survey.

According to claim 1 or 6 described a kind of pesticides to branch class sucking pest Toxicity Determination device, it is characterized in that: described bottom, give birth to survey the cover square, give birth to survey cover, column container is made by transparent plastic material.

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