CN103223008A - Method for extracting high-purity pueraria flavone by using water as solvent - Google Patents
Method for extracting high-purity pueraria flavone by using water as solvent Download PDFInfo
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- CN103223008A CN103223008A CN2013101922416A CN201310192241A CN103223008A CN 103223008 A CN103223008 A CN 103223008A CN 2013101922416 A CN2013101922416 A CN 2013101922416A CN 201310192241 A CN201310192241 A CN 201310192241A CN 103223008 A CN103223008 A CN 103223008A
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Abstract
The invention relates to a method for extracting high-purity pueraria flavone by using water as solvent, which comprises the following steps: extracting by using water as solvent, performing adsorption elution on the obtained pueraria flavone extractum through an HPD-600 macroporous resin, concentrating, and drying to finally obtain pueraria flavone of which the purity is more than 95%. According to the invention, pueraria flavone is extracted by using water instead of organic solvent as solvent, thus obtaining the high-purity pueraria flavone. The invention solves the organic residue problem, reduces the environmental pollution, lowers the production cost and improves the work efficiency.
Description
Technical field
The invention belongs to the Chinese medicine extraction preparing technical field, especially a kind of is the method that solvent extracts the high-purity Radix Puerariae flavone with water.
Background technology
Radix Puerariae (Radix puerariae) is the dry root of legume pueraria lobata Pueraria lobata (Wiolld) Ohwi, and its sweet in the mouth, suffering, property are put down.Radix Puerariae is a kind of Chinese medicine of China, medical value for Radix Puerariae is just on the books in Shennong's Herbal, its main component is a starch, contains 12% the flavone compound of having an appointment in addition, comprises kind surplus Semen sojae atricolor (Semen Glycines) glycoside, daidzein, the puerarin etc. 10; And contain daucosterol, aminoacid, Coumarins etc.Oneself is regarded as the mountain region plant of medicine-food two-purpose to Radix Puerariae by Ministry of Public Health at present.
Radix Puerariae has functions such as spasmolytic is brought down a fever, promoted the production of body fluid, rash, yang invigorating antidiarrheal.Similar with other most of flavone compounds, the Radix Puerariae flavone compounds also has multiple pharmacological function, as improves blood circulation, blood pressure lowering, blood sugar lowering, blood fat reducing, inhibition arteriosclerosis, anti-arrhythmia, antiplatelet aggregation, antitumor, anti-hypoxia and antioxidation etc.Oneself is widely used in fields such as medical industry, food industry, daily-use chemical industry to Radix Puerariae total flavones because of its significant medical value now.
In recent years, characteristics such as the natural plants active ingredient is obvious because of its effect, natural low toxicity gain great popularity, and have caused worldwide and have used the natural herb plant to prevent and treat the upsurge of various diseases.Along with carrying out in a deep going way to natural plant crude drugs research, the research range of Radix Puerariae has had further expansion, wherein the research of extraction of the flavone of Radix Puerariae and purification has been subjected to extensive concern, the main method of the extraction of Radix Puerariae total flavones has at present: (1) alcohol reflux method, mainly with water, ethanol, alkaline alcohol, lime water, methanol, acetone etc. as solvent, reflux.Reflux, extract, 2～3 times is extracted Radix Puerariae total flavones; (2) alcohol percolation method; (3) infusion process; (4) microwave auxiliary extraction method; (5) enzymolysis circumfluence method; (6) ultrasonic counter-current extraction; (7) ion coordination extraction.
Market is increasing to the demand of Radix Puerariae flavone.But because the deficiency of input in science and technology, domestic Radix Puerariae flavone preparation technique is backward relatively, and the extracting method of Radix Puerariae flavone mostly is organic solvent extraction, and production efficiency is low, energy consumption is big, environmental pollution is serious, impurity content is high, and product lacks the market competitiveness.Doing solvent with water to replace organic solvent carries out high-purity extraction to Radix Puerariae flavone and has solved organic residue problem.
Find one piece of patent documentation related to the present invention by retrieval, it is a kind of preparation method (CN101953880A) of effective component of kudzu root, mainly may further comprise the steps: 1) Radix Puerariae immerses in the water and decocts; 2) separate melt cinder; 3) extracting solution column chromatography, the water eluting; 4) concentrate drying.Above-mentioned patent documentation is the technical improvement of extracting purification at Radix Puerariae flavone, but with the technical scheme of present patent application in essence different is arranged.
Summary of the invention
The objective of the invention is to overcome the deficiencies in the prior art part, providing a kind of is the method that solvent extracts the high-purity Radix Puerariae flavone with water.
The objective of the invention is to be achieved through the following technical solutions:
(1) Radix Puerariae flavone extracts: exsiccant Radix Puerariae phloem is ground into coarse powder mixes with water, and in 1.5MPa, 60 ℃ of hot dipping 10h.Filter filtrate for later use.Adding waits water gaging in filtering residue again, and hot dipping 15h under uniform temp and the pressure condition filters 3 times repeatedly.At vacuum＜0.1MPa, filtrate concentrating obtained Radix Puerariae flavone extractum under 45～60 ℃, effective component extraction rate reaches more than 90%;
(2) Radix Puerariae flavone purification: the Radix Puerariae flavone extractum water-soluble liquid that obtains through the HPD-600 macroporous resin adsorb, eluting.Earlier with water-solubility impurities such as distilled water flush away saccharide, protein, tannins, use 60% ethanol elution then instead during eluting, eluent is concentrated and reclaim ethanol.The concentrated solution drying is obtained purity reach Radix Puerariae flavone more than 95%.
And the mass ratio of Radix Puerariae phloem coarse powder and water is 1g: 8mL in the described step (1).
And the condition of HPD-600 macroporous resin is in the described step (1): blade diameter length ratio is 1: 6, last sample concentration 50mg/mL, and maximum applied sample amount is 3BV, flow velocity is 1mL/min, eluant consumption 5BV.
Advantage of the present invention and good effect are:
The present invention is solvent with water, extracts to obtain the high-purity Radix Puerariae flavone.This method water replaces in the past organic solvent to do solvent, has reduced organic residually and to the pollution of environment, has reduced production cost, has improved work efficiency.Operation safety, be connected rationally simple and direct, be easy to promote.
The specific embodiment
Below in conjunction with embodiment, the present invention is further described, and following embodiment is illustrative, is not determinate, can not limit protection scope of the present invention with following embodiment.
Instantiation of the present invention:
1,500g Radix Puerariae phloem is ground into coarse powder and mixes with the distilled water of 4L, in 1.5MPa, and 60 ℃ of hot dipping 10h.Filter filtrate for later use.Adding waits water gaging in filtering residue again, and hot dipping 15h under uniform temp and the pressure condition filters 3 times repeatedly.At vacuum＜0.1MPa, filtrate concentrating obtained Radix Puerariae flavone extractum under 45～60 ℃, effective component extraction rate reaches more than 90%.
2, the Radix Puerariae flavone extractum that obtains is mixed with the solution of 50mg/mL, through the HPD-600 macroporous resin adsorb, eluting.HPD-600 macroporous resin blade diameter length ratio is 1: 6, and maximum applied sample amount is 3BV, and flow velocity is 1mL/min, eluant consumption 5BV.Earlier with water-solubility impurities such as distilled water flush away saccharide, protein, tannins, use 60% ethanol elution then instead during eluting, eluent is concentrated and reclaim ethanol.Obtain purity after the concentrated solution drying and reach Radix Puerariae flavone more than 95%.
Claims (3)
1. one kind is the method that solvent extracts the high-purity Radix Puerariae flavone with water, it is characterized in that:
Step is:
(1) Radix Puerariae flavone extracts: exsiccant Radix Puerariae phloem is ground into coarse powder mixes with water, and in 1.5MPa, 60 ℃ of hot dipping 10h.Filter filtrate for later use.Adding waits water gaging in filtering residue again, and hot dipping 15h under uniform temp and the pressure condition filters 3 times repeatedly.At vacuum＜0.1MPa, filtrate concentrating obtained Radix Puerariae flavone extractum under 45～60 ℃, effective component extraction rate reaches more than 90%;
(2) Radix Puerariae flavone purification: the Radix Puerariae flavone extractum water-soluble liquid that obtains through the HPD-600 macroporous resin adsorb, eluting.Earlier with water-solubility impurities such as distilled water flush away saccharide, protein, tannins, use 60% ethanol elution then instead during eluting, eluent is concentrated and reclaims ethanol, the concentrated solution drying is obtained purity greater than 95% Radix Puerariae flavone.
2. according to claim 1 is the method that solvent extracts the high-purity Radix Puerariae flavone with water, it is characterized in that: the mass ratio of Radix Puerariae phloem coarse powder and water is 1g: 8mL in the described step (1).
3. according to claim 1 is the method that solvent extracts the high-purity Radix Puerariae flavone with water, it is characterized in that: the condition of HPD-600 macroporous resin is in the described step (1): blade diameter length ratio is 1: 6, last sample concentration 50mg/mL, maximum applied sample amount is 3BV, flow velocity is 1mL/min, eluant consumption 5BV.
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| Application Number | Priority Date | Filing Date | Title |
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| CN2013101922416A CN103223008A (en) | 2013-05-22 | 2013-05-22 | Method for extracting high-purity pueraria flavone by using water as solvent |
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| CN2013101922416A CN103223008A (en) | 2013-05-22 | 2013-05-22 | Method for extracting high-purity pueraria flavone by using water as solvent |
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Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1524867A (en) * | 2003-02-27 | 2004-09-01 | 上海中医药大学 | A kind of extraction method of kudzu root total flavonoids |
| CN1927876A (en) * | 2005-09-07 | 2007-03-14 | 天津天士力现代中药资源有限公司 | Preparation method of puerarin extract |
| CN101485712A (en) * | 2008-01-15 | 2009-07-22 | 上海中医药大学附属龙华医院 | Method for preparing kudzu root flavone |
-
2013
- 2013-05-22 CN CN2013101922416A patent/CN103223008A/en active Pending
Patent Citations (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN1524867A (en) * | 2003-02-27 | 2004-09-01 | 上海中医药大学 | A kind of extraction method of kudzu root total flavonoids |
| CN1927876A (en) * | 2005-09-07 | 2007-03-14 | 天津天士力现代中药资源有限公司 | Preparation method of puerarin extract |
| CN101485712A (en) * | 2008-01-15 | 2009-07-22 | 上海中医药大学附属龙华医院 | Method for preparing kudzu root flavone |
Non-Patent Citations (3)
| Title |
|---|
| 张爱丽等: "常压和加压浸渍法制备壳聚糖性能对比", 《化工进展》 * |
| 李岂凡等: "葛根总黄酮的分离与纯化", 《南昌大学学报(理科版)》 * |
| 邹忠梅等: "大孔吸附树脂对葛根总黄酮的吸附研究", 《湖北中医学院学报》 * |
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Application publication date: 20130731 |