CN103222540A - Composite micro-ecological fish feed additive - Google Patents

Abstract

The invention relates to a compound micro-ecological fish feed additive, which is composed of materials, compound bacterial liquid, prebiotics and sterile water, wherein: the added amount of the compound bacterial liquid is 1.5‰ of the weight of the material, and the added amount of the prebiotic is 1.5‰ of the weight of the material 1.0% of the weight, and the amount of sterile water added is 35-50% of the weight of the material; the compound micro-ecological fish feed additive of the present invention has a probiotic content of more than 10 ¹¹ cfu/g, and the probiotics produce a large amount of protease, starch Enzymes, lipases, active peptides. In addition, the strains have been acclimated to temperature and have high thermal stability; three kinds of prebiotic polysaccharides have been added to improve the viability of probiotics in the intestinal tract of fish, so that the loss rate of probiotics is below 10%. The content of probiotics in feces has also been greatly improved, which can purify water bodies and reduce fish production costs.

Description

A kind of compound microecological fish feed additive and its preparation process

technical field

The invention relates to the technical field of aquaculture feed additives, in particular to a compound microecological fish feed additive.

Background technique

In recent years, people have paid great attention to dietary hygiene and drug residues. They have seriously affected people's health. Starting from the source, it naturally involves various animal feeds. Some European countries have realized this in the 19th century. , They have strictly controlled feed additives, for example, Denmark strictly prohibits adding antibiotics to feed. Therefore, microecological feed has been widely used abroad.

The domestic consumption of fish products is very large, so the greenness of fish feed is directly related to people's health, and antibiotic additives are still used in many fish farms, and the compound microecological fish feed Additives can solve this problem, can completely replace antibiotics, improve fish immunity and reduce diseases, and the prebiotics in the compound preparation can promote the balance of fish intestinal flora, promote fish metabolic growth, increase production, and can To solve the problem of fish excrement polluting the water body, the probiotics in the compound preparation can improve the water body environment and reduce the production cost. Therefore, the compound microecological feed additive is the development trend of feed additive application.

Contents of the invention

The purpose of the present invention is to solve the deficiencies of the above technical problems, to provide a compound micro-ecological fish feed additive and its preparation process, the combined micro-ecological fish feed additive product can replace antibiotics, improve fish immunity, improve water body environment, Promote fish growth.

In order to solve the deficiencies of the above-mentioned technical problems, the technical solution adopted by the present invention is: a compound micro-ecological fish feed additive, which is composed of materials, compound bacterial liquid, prebiotics and sterile water, wherein: the added amount of the compound bacterial liquid 1.5‰ of the weight of the material, the amount of prebiotics added is 1.0% of the weight of the material, and the amount of sterile water added is 35-50% of the weight of the material;

The materials are composed of 10-25 parts by weight of fish meal, 40-60 parts of soybean meal powder, 30-45 parts of bran, 25-35 parts of corn flour, 1-3 parts of vegetable oil and 1-2 parts of sodium chloride ;

The composite bacterial solution is composed of 0.3-1.0 parts of protease-producing Bacillus subtilis, 0.3-1.0 parts of amylase-producing Bacillus subtilis, 0.3-1.0 parts of lipase-producing Bacillus subtilis, and 0.5-1.0 parts of lipase-producing Bacillus subtilis in parts by weight. 1.0-1.8 parts of Enterococcus faecalis, 1.8-2.5 parts of Candida utilis, 0.8-1.5 parts of Geotrichum candidum, 0.8-1.5 parts of Bifidobacterium and 1.5-2.5 parts;

The prebiotics are composed of 3-10 parts of fructo-oligosaccharide, 6-12 parts of galacto-oligosaccharide and 5-15 parts of mannose-oligosaccharide in parts by weight.

The said compound micro-ecological fish feed additive is added to fish feed at a ratio of 5% to 9% by weight.

A preparation process for a compound microecological fish feed additive, comprising the following steps:

1) Take 10-25 parts of fish meal, 40-60 parts of soybean meal powder, 30-45 parts of bran, 25-35 parts of corn flour, 1-3 parts of vegetable oil and 1-2 parts of sodium chloride according to the ratio of parts by weight. The solid material is pulverized and passed through an 80-mesh sieve, and then the components are mixed and stirred evenly to obtain the material, which is set aside;

2) Cultivate the strains required for the composite bacterial solution separately by conventional methods, and take 0.3-1.0 parts of protease-producing Bacillus subtilis, 0.3-1.0 parts of amylase-producing Bacillus subtilis, and lipase-producing Bacillus subtilis in parts by weight 0.3～1.0 parts, Bacillus cereus 0.5～1.0 parts, Enterococcus faecalis 1.0～1.8 parts, Candida utilis 1.8～2.5 parts, Geotrichum candidum 0.8～1.5 parts, Bifidobacterium 0.8～1.5 parts and Lactic acid bacteria 1.5～ 2.5 parts, first mix and culture Bacillus subtilis producing protease, Bacillus subtilis producing amylase, Bacillus subtilis producing lipase, Bacillus cereus, Candida utilis and Geotrichum candidum for 3 hours, then add Enterococcus faecalis, Bi Mycobacterium and lactic acid bacteria, obtain composite bacteria liquid, for subsequent use;

3) Take 3-10 parts of fructo-oligosaccharides, 6-12 parts of galacto-oligosaccharides and 5-15 parts of mannose-oligosaccharides according to the ratio of parts by weight, and mix them evenly to obtain prebiotics for later use;

4) Take a composite bacterial solution with a material weight of 1.5‰ and a sterile water with a material weight of 35-50%, add the sterile water to the composite bacterial solution, and stir evenly to obtain a diluted composite bacterial solution; another material with a weight of 1.0% The prebiotics spare;

5) Add the diluted composite bacterial solution obtained in step 4) to the material obtained in step 1), stir evenly, and aerobically ferment at 35-40°C for 48-72 hours;

6) After the fermentation is over, dry the mixture at a temperature lower than 45°C until the moisture content is 4% to 8%, and then crush it through a 30-mesh sieve;

7) Mix the pulverized mixture with the prebiotics taken out in step 4), stir evenly, and pack to obtain the finished compound microecological fish feed additive.

Beneficial effect

In the compound microecological fish feed additive of the invention, the content of probiotics exceeds 10 ¹¹ cfu/g, and the probiotics produce a large amount of protease, amylase, lipase and active peptide. In addition, the strains have been acclimated to temperature and have high thermal stability; three kinds of prebiotic polysaccharides have been added to improve the viability of probiotics in the intestinal tract of fish, so that the loss rate of probiotics is below 10%. The loss rate of micro-ecological feed on the market is generally above 30%, and the content of probiotics in fish feces has also been greatly improved, which can purify water and reduce fish production costs.

Detailed ways

A compound micro-ecological fish feed additive, which is composed of materials, compound bacteria solution, prebiotics and sterile water, wherein: the addition amount of the compound bacteria solution is 1.5‰ of the weight of the material, and the addition amount of the prebiotics is 1.0% of the weight of the material %, the amount of sterile water added is 35-50% of the weight of the material;

The materials are composed of 10-25 parts by weight of fish meal, 40-60 parts of soybean meal powder, 30-45 parts of bran, 25-35 parts of corn flour, 1-3 parts of vegetable oil and 1-2 parts of sodium chloride ;

The composite bacterial solution is composed of 0.3-1.0 parts of protease-producing Bacillus subtilis, 0.3-1.0 parts of amylase-producing Bacillus subtilis, 0.3-1.0 parts of lipase-producing Bacillus subtilis, and 0.5-1.0 parts of lipase-producing Bacillus subtilis in parts by weight. 1.0-1.8 parts of Enterococcus faecalis, 1.8-2.5 parts of Candida utilis, 0.8-1.5 parts of Geotrichum candidum, 0.8-1.5 parts of Bifidobacterium and 1.5-2.5 parts;

Protease-producing Bacillus subtilis, amylase-producing Bacillus subtilis and lipase-producing Bacillus subtilis are respectively screened out from Bacillus subtilis to produce high-yield protease, high-yield amylase and high-yield lipase, and the screening method is a conventional method .

The prebiotics are composed of 3-10 parts of fructo-oligosaccharide, 6-12 parts of galacto-oligosaccharide and 5-15 parts of mannose-oligosaccharide in parts by weight.

The said compound micro-ecological fish feed additive is added to fish feed at a ratio of 5% to 9% by weight.

A preparation process for a compound microecological fish feed additive, comprising the following steps:

1) Take 10-25 parts of fish meal, 40-60 parts of soybean meal powder, 30-45 parts of bran, 25-35 parts of corn flour, 1-3 parts of vegetable oil and 1-2 parts of sodium chloride according to the ratio of parts by weight. The solid material is pulverized and passed through an 80-mesh sieve, and then the components are mixed and stirred evenly to obtain the material, which is set aside;

2) Cultivate the strains required for the composite bacterial solution separately by conventional methods, and take 0.3-1.0 parts of protease-producing Bacillus subtilis, 0.3-1.0 parts of amylase-producing Bacillus subtilis, and lipase-producing Bacillus subtilis in parts by weight 0.3～1.0 parts, Bacillus cereus 0.5～1.0 parts, Enterococcus faecalis 1.0～1.8 parts, Candida utilis 1.8～2.5 parts, Geotrichum candidum 0.8～1.5 parts, Bifidobacterium 0.8～1.5 parts and Lactic acid bacteria 1.5～ 2.5 parts, first mix and culture Bacillus subtilis producing protease, Bacillus subtilis producing amylase, Bacillus subtilis producing lipase, Bacillus cereus, Candida utilis and Geotrichum candidum for 3 hours, then add Enterococcus faecalis, Mycobacterium and lactic acid bacteria, obtain composite bacteria liquid, for subsequent use;

3) Take 3-10 parts of fructo-oligosaccharides, 6-12 parts of galacto-oligosaccharides and 5-15 parts of mannose-oligosaccharides according to the ratio of parts by weight, and mix them evenly to obtain prebiotics for later use;

4) Take a composite bacterial solution with a material weight of 1.5‰ and a sterile water with a material weight of 35-50%, add the sterile water to the composite bacterial solution, and stir evenly to obtain a diluted composite bacterial solution; another material with a weight of 1.0% The prebiotics spare;

5) Add the diluted composite bacterial solution obtained in step 4) to the material obtained in step 1), stir evenly, and aerobically ferment at 35-40°C for 48-72 hours;

6) After the fermentation is over, dry the mixture at a temperature lower than 45°C until the moisture content is 4% to 8%, and then crush it through a 30-mesh sieve;

7) Mix the pulverized mixture with the prebiotics taken out in step 4), stir evenly, and pack to obtain the finished compound microecological fish feed additive.

The following are specific embodiments of the present invention:

Example 1

A preparation method for a compound microecological fish feed additive, comprising the following steps:

1) Take 10 parts of fish meal, 40 parts of soybean meal powder, 30 parts of bran, 25 parts of corn flour, 1 part of vegetable oil and 1 part of sodium chloride according to the ratio of parts by weight, crush the solid materials, and pass through an 80-mesh sieve , and then mix the components and stir evenly to obtain the material, which is set aside;

2) Use conventional methods to separately cultivate the strains required for the composite bacterial solution, and take 0.3 parts of protease-producing Bacillus subtilis, 0.3 parts of amylase-producing Bacillus subtilis, 0.3 parts of lipase-producing Bacillus subtilis, wax 0.5 parts of Bacillus subtilis, 1.0 parts of Enterococcus faecalis, 1.8 parts of Candida utilis, 0.8 parts of Geotrichum candidum, 0.8 parts of Bifidobacterium and 1.5 parts of lactic acid bacteria. Lipase-producing Bacillus subtilis, Bacillus cereus, Candida utilis and Geotrichum candidum were mixed and sealed for 3 hours, and then Enterococcus faecalis, Bifidobacterium and lactic acid bacteria were added to obtain a composite bacterial solution for subsequent use;

3) Take 3 parts of fructo-oligosaccharides, 6 parts of galacto-oligosaccharides and 5 parts of mannose-oligosaccharides according to the ratio of parts by weight, and mix them evenly to obtain prebiotics for later use;

4) Take a compound bacterial solution with a weight of 1.5‰ of the material and sterile water with a weight of 35% of the material, add the sterile water to the compound bacterial solution, and stir evenly to obtain a diluted composite bacterial solution; another probiotic solution with a weight of 1.0% of the material Yuan spare;

5) Add the diluted composite bacteria solution obtained in step 4) to the material obtained in step 1), stir evenly, and aerobically ferment at 35-40°C for 48 hours;

6) After the fermentation is over, dry the mixture at a temperature lower than 45°C until the moisture content is 4% to 8%, and then crush it through a 30-mesh sieve;

7) Mix the pulverized mixture with the prebiotics taken out in step 4), stir evenly, and pack to obtain the finished compound microecological fish feed additive.

Example 2

A preparation method for a compound microecological fish feed additive, comprising the following steps:

1) Take 25 parts of fish meal, 60 parts of soybean meal powder, 45 parts of bran, 35 parts of corn flour, 3 parts of vegetable oil and 2 parts of sodium chloride according to the ratio of parts by weight, crush the solid materials, and pass through an 80-mesh sieve , and then mix the components and stir evenly to obtain the material, which is set aside;

2) Use conventional methods to separately cultivate the strains required for the composite bacterial solution, and take 1.0 parts of protease-producing Bacillus subtilis, 1.0 parts of amylase-producing Bacillus subtilis, 1.0 parts of lipase-producing Bacillus subtilis, wax 1.0 parts of Bacillus subtilis, 1.8 parts of Enterococcus faecalis, 2.5 parts of Candida utilis, 1.5 parts of Geotrichum candidum, 1.5 parts of Bifidobacterium and 2.5 parts of lactic acid bacteria. Lipase-producing Bacillus subtilis, Bacillus cereus, Candida utilis and Geotrichum candidum were mixed and sealed for 3 hours, and then Enterococcus faecalis, Bifidobacterium and lactic acid bacteria were added to obtain a composite bacterial solution for subsequent use;

3) Take 10 parts of fructo-oligosaccharides, 12 parts of galacto-oligosaccharides and 15 parts of mannose-oligosaccharides according to the ratio of parts by weight, and mix them evenly to obtain prebiotics for later use;

4) Take 1.5‰ composite bacterial solution with material weight and 50% sterile water with material weight, add sterile water into the composite bacterial solution, stir evenly to obtain diluted composite bacterial solution; take another probiotic solution with 1.0% material weight Yuan spare;

5) Add the diluted composite bacteria solution obtained in step 4) to the material obtained in step 1), stir evenly, and aerobically ferment at 35-40°C for 72 hours;

6) After the fermentation is over, dry the mixture at a temperature lower than 45°C until the moisture content is 4% to 8%, and then crush it through a 30-mesh sieve;

7) Mix the pulverized mixture with the prebiotics taken out in step 4), stir evenly, and pack to obtain the finished compound microecological fish feed additive.

Example 3

A preparation method for a compound microecological fish feed additive, comprising the following steps:

1) Take 15 parts of fish meal, 45 parts of soybean meal powder, 35 parts of bran, 30 parts of corn flour, 2 parts of vegetable oil and 1.5 parts of sodium chloride according to the ratio of parts by weight, crush the solid materials, and pass through an 80-mesh sieve , and then mix the components and stir evenly to obtain the material, which is set aside;

2) Use conventional methods to separately cultivate the strains required for the composite bacterial solution, and take 0.6 parts of protease-producing Bacillus subtilis, 0.7 parts of amylase-producing Bacillus subtilis, 0.5 parts of lipase-producing Bacillus subtilis, wax 0.9 parts of Bacillus subtilis, 1.5 parts of Enterococcus faecalis, 2.2 parts of Candida utilis, 1.2 parts of Geotrichum candidum, 1.0 parts of Bifidobacterium and 2.0 parts of lactic acid bacteria. Lipase-producing Bacillus subtilis, Bacillus cereus, Candida utilis and Geotrichum candidum were mixed and sealed for 3 hours, and then Enterococcus faecalis, Bifidobacterium and lactic acid bacteria were added to obtain a composite bacterial solution for subsequent use;

3) Take 7 parts of fructo-oligosaccharides, 9 parts of galacto-oligosaccharides and 10 parts of mannose-oligosaccharides according to the ratio of parts by weight, and mix them evenly to obtain prebiotics for later use;

4) Take a composite bacterial solution with a weight of 1.5‰ of the material and sterile water with a weight of 40% of the material, add the sterile water into the composite bacterial solution, and stir evenly to obtain a diluted composite bacterial solution; another probiotic solution with a weight of 1.0% of the material Yuan spare;

5) Add the diluted composite bacterial solution obtained in step 4) to the material obtained in step 1), stir evenly, and aerobically ferment at 37°C for 60 hours;

6) After the fermentation is over, dry the mixture at a temperature lower than 45°C until the moisture content is 4% to 8%, and then crush it through a 30-mesh sieve;

7) Mix the pulverized mixture with the prebiotics taken out in step 4), stir evenly, and pack to obtain the finished compound microecological fish feed additive.

Example 4

A preparation method of a compound microecological fish feed additive, comprising the following steps:

1) Take 25 parts of fish meal, 60 parts of soybean meal powder, 30 parts of bran, 35 parts of corn flour, 3 parts of vegetable oil and 1 part of sodium chloride according to the ratio of parts by weight, crush the solid materials, and pass through an 80-mesh sieve , and then mix the components and stir evenly to obtain the material, which is set aside;

2) Use conventional methods to separately cultivate the strains required for the composite bacterial solution, and take 1.0 part of protease-producing Bacillus subtilis, 1.0 part of amylase-producing Bacillus subtilis, 0.4 part of lipase-producing Bacillus subtilis, wax 0.5 parts of Bacillus subtilis, 1.6 parts of Enterococcus faecalis, 1.9 parts of Candida utilis, 1.3 parts of Geotrichum candidum, 1.2 parts of Bifidobacterium and 1.8 parts of lactic acid bacteria. Lipase-producing Bacillus subtilis, Bacillus cereus, Candida utilis and Geotrichum candidum were mixed and sealed for 3 hours, and then Enterococcus faecalis, Bifidobacterium and lactic acid bacteria were added to obtain a composite bacterial solution for subsequent use;

3) Take 6 parts of fructo-oligosaccharides, 6 parts of galacto-oligosaccharides and 9 parts of mannose-oligosaccharides according to the ratio of parts by weight, and mix them evenly to obtain prebiotics for later use;

4) Take a compound bacterial solution with a weight of 1.5‰ of the material and sterile water with a weight of 45% of the material, add the sterile water to the compound bacterial solution, and stir evenly to obtain a diluted composite bacterial solution; take another probiotic with a weight of 1.0% of the material Yuan spare;

5) Add the diluted composite bacteria liquid obtained in step 4) to the material obtained in step 1), stir evenly, and aerobically ferment at 40°C for 55 hours;

6) After the fermentation is over, dry the mixture at a temperature lower than 45°C until the moisture content is 4% to 8%, and then crush it through a 30-mesh sieve;

7) Mix the pulverized mixture with the prebiotics taken out in step 4), stir evenly, and pack to obtain the finished compound microecological fish feed additive.

Claims (3)

1. A compound micro-ecological fish feed additive, characterized in that: it is made up of material, compound bacteria liquid, prebiotic and sterile water, wherein: the addition of compound bacteria liquid is 1.5‰ of material weight, and the addition of prebiotic The amount is 1.0% of the weight of the material, and the amount of sterile water added is 35-50% of the weight of the material; The materials are composed of 10-25 parts by weight of fish meal, 40-60 parts of soybean meal powder, 30-45 parts of bran, 25-35 parts of corn flour, 1-3 parts of vegetable oil and 1-2 parts of sodium chloride ; The composite bacterial solution is composed of 0.3-1.0 parts of protease-producing Bacillus subtilis, 0.3-1.0 parts of amylase-producing Bacillus subtilis, 0.3-1.0 parts of lipase-producing Bacillus subtilis, and 0.5-1.0 parts of lipase-producing Bacillus subtilis in parts by weight. 1.0-1.8 parts of Enterococcus faecalis, 1.8-2.5 parts of Candida utilis, 0.8-1.5 parts of Geotrichum candidum, 0.8-1.5 parts of Bifidobacterium and 1.5-2.5 parts; The prebiotics are composed of 3-10 parts of fructo-oligosaccharide, 6-12 parts of galacto-oligosaccharide and 5-15 parts of mannose-oligosaccharide in parts by weight. 2. The compound micro-ecological fish feed additive according to claim 1, characterized in that: said compound micro-ecological fish feed additive is added to fish feed in a proportion of 5% to 9% by weight. 3. the preparation technology of compound probiotic fish feed additive as claimed in claim 1, is characterized in that: comprise the following steps: 1) Take 10-25 parts of fish meal, 40-60 parts of soybean meal powder, 30-45 parts of bran, 25-35 parts of corn flour, 1-3 parts of vegetable oil and 1-2 parts of sodium chloride according to the ratio of parts by weight. The solid material is pulverized and passed through an 80-mesh sieve, and then the components are mixed and stirred evenly to obtain the material, which is set aside; 2) Cultivate the strains required for the composite bacterial solution separately by conventional methods, and take 0.3-1.0 parts of protease-producing Bacillus subtilis, 0.3-1.0 parts of amylase-producing Bacillus subtilis, and lipase-producing Bacillus subtilis in parts by weight 0.3～1.0 parts, Bacillus cereus 0.5～1.0 parts, Enterococcus faecalis 1.0～1.8 parts, Candida utilis 1.8～2.5 parts, Geotrichum candidum 0.8～1.5 parts, Bifidobacterium 0.8～1.5 parts and Lactic acid bacteria 1.5～ 2.5 parts, first mix and culture Bacillus subtilis producing protease, Bacillus subtilis producing amylase, Bacillus subtilis producing lipase, Bacillus cereus, Candida utilis and Geotrichum candidum for 3 hours, then add Enterococcus faecalis, Bi Mycobacterium and lactic acid bacteria, obtain composite bacteria liquid, for subsequent use; 3) Take 3-10 parts of fructo-oligosaccharides, 6-12 parts of galacto-oligosaccharides and 5-15 parts of mannose-oligosaccharides according to the ratio of parts by weight, and mix them evenly to obtain prebiotics for later use; 4) Take a compound bacterial solution with a weight of 1.5‰ of the material and sterile water with a weight of 35-50% of the material, add the sterile water into the compound bacterial solution, and stir evenly to obtain a diluted composite bacterial solution; take another 1.0% of the material weight The prebiotics spare; 5) Add the diluted composite bacterial solution obtained in step 4) to the material obtained in step 1), stir evenly, and aerobically ferment at 35-40°C for 48-72 hours; 6) After the fermentation is over, dry the mixture at a temperature lower than 45°C until the moisture content is 4% to 8%, and then crush it through a 30-mesh sieve; 7) Mix the pulverized mixture with the prebiotics taken out in step 4), stir evenly, and pack to obtain the finished compound microecological fish feed additive.