CN103210881A - Large-scale artificial breeding method for Neoseiulu californicus - Google Patents

Abstract

The invention relates to a large-scale artificial breeding method of Neoseius californica of a drought-resistant strain, which establishes mutually isolated plant cultivation rooms, prey breeding rooms and predatory mite breeding rooms; a self-controlled constant temperature glass incubator is placed in each room to maintain the temperature 25°C±1°C, relative humidity 40%-50%, photoperiod 16L:8D, place a tray in the incubator; place 16 incubators in the tray, and spread a 5-7 cm thick culture medium in the incubator; Then cultivate the prey host plant in the glass incubator in the plant cultivation room, and the prey host plant will germinate and grow the first compound leaf for later use; the cultivated prey host plant will be moved into the prey breeding room and inserted into the Turkestan spider mite to raise the prey; About 15 days after the prey host plant was inoculated with the Turkestan spider mite, the culture plate was moved into the predator mite breeding room for inoculation and rearing of Neoseius californica; Collection of mites. It can guarantee the long-term supply of Neoseius californica.

Description

Method for Large-scale Artificial Breeding of Neoseius californica

technical field

The invention belongs to the field of biological control of agricultural pests; more specifically, the invention relates to a method for large-scale artificial breeding of a drought-tolerant strain of Neoseiius californica. the

technical background

Neoseiulu californicus (McGregor) belongs to the Acari subclass Acari, Phytoseii-Dae Phytoseii-Dae, Neoseiulu genus Neoseiulu, its natural populations are widely distributed in Argentina, Chile, California, Florida, Texas Sri Lanka, Japan, South Africa, southern Europe and the Mediterranean coast. It inhabits a wide variety of crops, such as avocados, citrus and other fruit trees, as well as cassava, corn, grapes, herbaceous plants, some vegetable crops and ornamental plants, and is a species that has a strong natural control effect on harmful mites important natural enemy. the

Neoseius californica has a wide range of predation and can prey on a variety of harmful mites, mainly including: Tetranychus cinnabarinus, Tetranychus truncates, Panonychus citrus, Eotetranychus citrus, tarsus polyphagous, apple whole Claw mites, tea orange gall mites, etc. the

At present, there are many commercial predatory mites on the market, but it is difficult to control agricultural pest mites on dry crops. Neoseius californica adapts to a wide range of humidity (from 25% to 90% relative humidity). The intraspecific variation caused by sensitivity to humidity can be used to screen drought-tolerant strains of Neoseiius californica. A.Walzer, P.Schausberger(2008) ¹ et al., E.Palevsky(2010) ² etc. on eight different strains of Neoseius californica (FR from Herault in France, FL from Tuscany in Italy, SP in Valencia in Spain , CH from LaCruz in Chile, SI from Sicily in Italy, and C-BOKU, C-ISZA, and C-ARO from California, USA), and found that the BOKU strain is the most suitable for the arid environment. Drought-tolerant strains of Seiusius militaris.

N. californicus has been used as a very effective biocontrol agent to control spider mite abroad. Due to the short development period of this mite, fast reproduction speed, long life span of female adults, wide range of predation and large food intake, using it to carry out biological control of harmful mites can significantly reduce the amount of pesticides used, reduce the amount of pesticide residues in agricultural products, and improve Yield and quality of agricultural products. The use of artificially propagated Neoseius californica to control agricultural pest mites is an ecologically friendly biological control method, and has broad application prospects in agricultural production, especially in the production of green and organic agricultural products. the

Contents of the invention

The purpose of the present invention is to: provide a method for large-scale artificial breeding of Neoseius californica of a drought-tolerant strain, which can ensure the continuous supply of food sources required for raising Neoseius california, and produce Neoseius californica in large quantities for a long time to meet the needs of agricultural production. the

The present invention is realized in the following manner: the large-scale artificial breeding method of Neoseius californica, the establishment of mutually isolated plant cultivation chambers, prey breeding chambers and predatory mite breeding chambers, the surroundings of each chamber are separated by water tanks, and water is regularly flushed in the water tanks , to prevent the escape of prey and predatory mites; maintain a temperature of 25°C±1°C, a humidity of 40%-50%, a photoperiod of 16L:8D, temperature, light and humidity control equipment including: computer, air conditioner, lighting equipment, humidifier, temperature and humidity Sensors, self-controlled constant temperature glass incubators, refrigerators; put trays on the glass incubators; place 16 incubators on each tray, spread 5-7cm thick medium in the incubators; then cultivate in the plant culture room Cultivate the prey host plant in the container. After the prey host plant germinates and grows the first compound leaf, it is ready for use; the prey host plant culture plate is moved into the prey breeding room, and the Turkestan spider mite is inserted to raise the prey; when the prey host plant is inserted About 15 days after Tetranychus turkestan, move the culture plate into the predatory mite breeding room for inoculation and rearing of Neoseius californica; 10 to 15 days after inoculation with Neoseius californica, collect Neoseius california. the

The food source host plant is cowpea, and the cultivation of the prey host plant is as follows: wash the cowpea seeds with running water for 10 minutes, soak them in alcohol for 30 seconds, rinse them twice with distilled water, then soak them in 0.1% arsenic and mercury for 8-10 minutes, rinse them with distilled water Three times, sow 5 to 8 cowpea seeds in each incubator, the depth is 1 to 2 cm, keep the medium humidity in the incubator, and wait for 7 to 10 days when the seeds germinate and grow the first compound leaf. the

The incubator is a 10cm×10cm black nutrition bag, and the culture medium is prepared by vermiculite:perlite at a ratio of 1:1 and sterilized by high temperature. the

The feeding of the prey specifically refers to transferring the cultured host plant to the prey cultivation room, placing the leaves with the Turkestan spider mite on the leaves of the cowpea, allowing the Turkestan spider mite to grow and reproduce, and to be used. the

The inoculation and rearing of Neoseius californica specifically refers to that when cowpea leaves are inoculated with Tetranychus Turkestan for about 15 days, the total number of adult mites and nymphs on the leaves of cowpea seedlings will multiply to an average of 150 to 300 heads per leaf, The culture plate was transferred to the predatory mite breeding room, and the leaves with Neoseius californica were cut into small pieces, and placed on the leaves of the cowpea plants that raised the Turkestan spider mite. The number of adult mite is 5 to 10. After 15 to 20 days of inoculation with Neoseius californica, the collection of Neoseius californica is carried out. the

The invention adopts vermiculite and perlite as the culture medium, which can be recycled after sterilization, uses cowpea as the prey host plant, and is easy to cultivate; the Turkestan spider mite is the prey, and the Turkestan spider mite is used as the food source of Neoseius californica. the

Detailed ways

Embodiments of the present invention: the artificial breeding method of Neoseius californica, set up mutually isolated plant cultivation chambers, prey breeding chambers and predatory mite breeding chambers, prey breeding chambers and predatory mite breeding chambers are isolated with water tanks around the chamber; in each chamber Place a self-controlled constant temperature glass incubator, keep the temperature at 25°C±1°C, humidity 40%-50%, and light cycle 16L:8D; put trays on the glass incubator, place 16 incubators on each tray, and spread in the incubator A culture medium with a thickness of 6-7cm; then cultivate cowpea in the incubator, specifically, wash the cowpea seeds with running water for 10 minutes, soak them in alcohol for 30 seconds, rinse them twice with distilled water, and then soak them in 0.1% arsenic and mercury for 8-10 minutes, Rinse with distilled water three times, sow 5 to 8 cowpea seeds in each incubator at a depth of 1 to 2 cm, keep the medium humidity in the incubator, 7 to 10 days after the seeds germinate and grow the first compound leaf, set aside The cowpea seedlings that will be cultivated are moved into the prey breeding room, and the Turkestan spider mite is inserted into the breeding of the prey, specifically, when the cowpea grows the first compound leaf, the bean sprouts are moved into the prey breeding room, and connected with Cowpea leaves, the spider mites will climb onto the host plant by themselves, allowing them to grow and multiply freely on it, and a large number of spider mite eggs, nymphs and adults will reproduce on the back of the leaf in about half a month. The culture plate was moved into the predatory mite breeding room for inoculation and rearing of Neoseius californica. Specifically, the culture plate was transferred to the predatory mite breeding room, and the leaves with Neoseius californica were cut into small pieces and placed in the breeding room of Turkestan. On the leaves of the cowpea plants of spider mites, the number of adults of Neoseius californica inoculated on each cowpea leaf was 5, and after 15 to 20 days of inoculation of Neoseius californica, the collection of Neoseius californica was carried out . the

references

1 A. Walzer, M. Castagnoli, S. Simoni, E. Palevsky & P. Schausberger (2008) Identification of a drought-adapted Neoseiulus californicus strain: egg hatchability, juvenile survival and oviposition at low humidity. Trends in Acarology: Proceedings of the ¹² International Congress.

2E. Palevsky et alia. (2010) Development of an economic rearing and transport system for an arid adapted strain of the predatory mite, Neoseiulus californicus, for spider mite control. Trends in Acarology: Proceedings of the ^12th International Congress.

Claims (5)

1. a method for the large-scale artificial breeding of Neoseius californica of drought-tolerant strain, it is characterized in that setting up mutually isolated plant cultivation room, prey breeding room and predatory mite breeding room; Guarantee the food required for the raising of Neoseius californica With the continuous supply of sources, Neoseius californica can be produced in batches in large quantities. 2. The concrete measure of the isolation described in claim 1 is to separate with a water tank around the prey breeding room and the predatory mite breeding room, and flush water regularly in the tank to prevent the escape of prey and predatory mite. 3. each chamber maintenance temperature 25 ℃ ± 1 ℃ described in claim 1, relative humidity 40%-50%, photoperiod 16L:8D; Place glass incubator in each chamber. 4. the described plant cultivation chamber of claim 1 cultivates plants to be cowpea, and the cultivation method is: put tray on the glass incubator; Every dish places 16 cultivators, spreads into the culture medium of thick 5～7cm in the cultivator; Rinse cowpea seeds with running water for 10 minutes, soak them in alcohol for 30 seconds, rinse them twice with distilled water, then soak them with 0.1% arsenic and mercury for 8-10 minutes, rinse them with distilled water three times, sow 5-8 grains of cowpea seeds in each incubator, The depth is 1-2cm, keep the medium humidity in the incubator, and wait for 7-10 days when the seeds germinate and grow the first compound leaf. 5. the method for the large-scale artificial breeding of Neoseiidi california described in claim 1 is characterized in that the prey host plant culture dish that grows first compound leaf is moved into the prey breeding room and inserts Turkestan spider mite to carry out prey cultivation Breeding; when the prey host plant was inoculated with Tetranychus turkestan for about 15 days, move the culture plate into the predator mite breeding room for inoculation and rearing of Neoseius californica; Collection of Minor seiusius mites.