CN103194372A - Method and device for filtering and subpackaging microorganism culture medium - Google Patents

Abstract

The invention discloses a method and device for filtering and packing microbial culture medium. A filter sieve is arranged on the upper part of a filter cup body, filter gauze is placed on the filter sieve, and an overflow nozzle under the filter sieve is arranged on the filter cup body; Pour the microbial culture medium from the top of the filter cup. After the microbial culture medium is filtered through the filter gauze, the filtrate is deposited at the bottom of the filter cup, and the deposited filtrate is poured into the sub-packaging container through the overflow nozzle, so as to complete the microbial Media filter aliquots. The present invention adopts the method of combining filtration and subpackage in the same filter cup, and the operation is simple, fast, time-saving and labor-saving during filtration; during subpackage, it is only necessary to directly flow out the microbial culture solution from the overflow nozzle, without Transfer to the funnel again, reducing the operating steps, and it is not easy to cool and solidify. It avoids the situation that the filtrate coagulates due to the slow speed of filling in the existing sub-packaging method.

Description

Method and device for filtering and distributing microbial culture medium

technical field

The invention relates to a filtering method and a device, in particular to a method and a device for filtering and packing microbial culture medium.

Background technique

In the process of making PDA, oat, rye and other medium in the laboratory, it is often necessary to filter the residue in the culture solution. The traditional method is to filter the prepared medium with filter paper or gauze while it is hot. When filtering with filter paper, fold the filter paper into a tile shape and spread it on a funnel to filter. This method is simple to operate, but the filtration speed is very slow, and the culture medium is easy to solidify. When filtering with gauze, fold it into 4 to 6 layers and put it directly on the mouth of the beaker. This method is faster, but the gauze is easy to fall into the beaker when filtering, and when the filtering volume is large, it is difficult for one person to operate, and two people are often required. to complete. When aliquoting the filtered culture solution, the common method is to transfer the culture solution into a funnel connected with a spring clip, and transfer it to an Erlenmeyer flask or a test tube by controlling the tightness of the spring clip. Due to the limited capacity of the funnel, if the operation is too slow, the culture solution will quickly cool and solidify. However, there is no simple device integrating medium filtration and subpackaging in the laboratory at present.

Contents of the invention

The technical problem to be solved by the present invention is to provide a method and device for filtering and packing microbial culture medium, which can complete the filtering and packing of microbial culture medium at one time, and has the advantages of simple structure, convenient use, and time-saving and labor-saving .

The present invention is achieved in this way: the method for filtering and subpackaging microbial culture medium is provided with a filter screen on the top of the filter cup body, and a filter gauze is placed on the filter screen, and an overflow mouth below the filter screen is set on the filter cup body; Pour the microbial culture medium from the top of the filter cup. After the microbial culture medium is filtered through the filter gauze, the filtrate is deposited on the bottom of the filter cup. Media filter aliquots.

The device for the method of filtering and packing microbial culture medium includes a filter cup body, an inwardly protruding plane ring is provided on the inner upper part of the filter cup body, and a filter screen is arranged in the filter cup body, and the top of the filter screen corresponds to the plane ring, A cup cover is arranged on the top of the filter cup body, an overflow spout is arranged on the side wall of the filter cup body, and the connection position between the overflow spout and the filter cup body is below the filter screen.

A handle is provided at a position corresponding to the overflow mouth. easy to use.

There is a scale line on the lower side wall of the filter bowl. Indicates the amount of culture solution in the cup, which is convenient for measuring, observing and adding water to make up to the required volume.

A lid is provided on the overflow spout. It can be closed when filtering and opened when dispensing.

An internal thread is provided on the outside of the filter cup top, and a corresponding external thread is provided on the inside of the cup cover. The threaded connection can ensure reliable connection between the filter cup body and the cup cover, good sealing effect and easy removal.

Due to the adoption of the above technical solution, compared with the prior art, the present invention adopts the method of combining filtration and subpackage in the same filter cup, and the operation is simple, fast, time-saving and labor-saving during filtration; during subpackage, only The microbial culture solution can be directly flowed out from the overflow nozzle without being transferred into the funnel again, which reduces the operation steps and is not easy to cool and solidify. It avoids the situation that the filtrate coagulates due to the slow speed of filling in the existing sub-packaging method. The method of the invention is simple and easy, the adopted device is easy to manufacture, the cost is low, and the use effect is good.

Description of drawings

Accompanying drawing 1 is a structural representation of the present invention;

Accompanying drawing 2 is the structural representation of the filter screen of the present invention.

Detailed ways

Embodiments of the present invention: the method for filtering and subpackaging microbial culture medium, a filter screen is set on the top of the filter cup body, and filter gauze is placed on the filter screen, and an overflow mouth that is below the filter screen is set on the filter cup body; The culture medium is poured from the top of the filter cup, and the microbial culture medium is filtered through the filter gauze, and the filtrate is deposited at the bottom of the filter cup, and the deposited filtrate is poured into the sub-packaging container through the overflow nozzle, thereby completing the microbial culture medium at one time filter packing.

The structure of the device of the method for filtering and subpackaging microbial culture medium is as shown in Figure 1, comprises filter cup body 1, and the inner upper part of filter cup body 1 is provided with inwardly protruding plane ring 7, is provided with in filter cup body 1 Filter sieve 2, the top of filter sieve 2 corresponds to the plane ring 7, a cup cover 3 is provided on the top of the filter cup body 1, an internal thread is provided on the outside of the filter cup body 1 top, and an inner thread is provided on the inside of the cup cover 3. The corresponding external thread is provided with an overflow nozzle 6 on the side wall of the filter cup body 1, and the connection position between the overflow nozzle 6 and the filter cup body 1 is below the filter screen 2; a handle is provided at the corresponding position of the overflow nozzle 6 5; there is a scale line 4 on the lower part of the side wall of the filter cup body 1; a cover is provided on the overflow nozzle 6.

When in use, remove the cup cover 3, and lay 4-6 layers of gauze on the filter screen 2, pour PDA or OA and other culture fluid on the gauze to filter, after the filter is completed, take out the filter screen 2, and add water to make up to The required volume, then tighten the lid 3, open the lid of the overflow mouth 6, hold the handle 5, pour the culture solution into the Erlenmeyer flask through the overflow mouth 6, if it is necessary to pour the test tube slope, connect a spring with a spring to the overflow mouth Clamp the rubber tube, pinch the spring clip loosely to let the culture solution flow into the test tube, which can avoid slow filling and solidification; the cup body can be used as a volume measuring cup when it is not filtered.

The filter cup body 1 is made of stainless steel or high-temperature-resistant plastic, and its shape is a cylinder with a volume of about 3000-4000mL. The specific size and shape can be designed according to requirements.

Claims (6)

1. A method for filtering and subpackaging microbial culture medium, characterized in that: a filter screen is set on the top of the filter cup body, and filter gauze is placed on the filter screen, and an overflow mouth that is below the filter screen is set on the filter cup body; Pour the microbial culture medium from the top of the filter cup. After the microbial culture medium is filtered through the filter gauze, the filtrate is deposited on the bottom of the filter cup. Media filter aliquots. 2. A device adopting the method of filtering and distributing microbial culture medium as claimed in claim 1, comprising a filter cup (1), characterized in that: the inner upper part of the filter cup (1) is provided with an inward bulge The plane ring (7) of the filter cup body (1) is provided with a filter screen (2), the top of the filter screen (2) corresponds to the plane ring (7), and the top of the filter cup body (1) is provided with a cup The cover (3) is provided with an overflow nozzle (6) on the side wall of the filter cup body (1), and the connection position between the overflow nozzle (6) and the filter cup body (1) is below the filter screen (2). 3. The device for filtering and dispensing microbial culture medium according to claim 2, characterized in that: a handle (5) is provided at the position corresponding to the overflow nozzle (6). 4. The device for filtering and distributing microbial culture medium according to claim 2, characterized in that there is a scale line (4) at the lower part of the side wall of the filter cup body (1). 5. The device for filtering and packing microbial culture medium according to claim 2, characterized in that: a cover is provided on the overflow nozzle (6). 6. The device for filtering and packaging microbial culture medium according to claim 2, characterized in that: an internal thread is provided on the outside of the top of the filter cup (1), and a corresponding thread is provided on the inside of the cup cover (3). external thread.

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