CN103169788A - Chinese date leaf extractive and application thereof in preparation of medicament or health food for preventing and treating liver injury - Google Patents
Chinese date leaf extractive and application thereof in preparation of medicament or health food for preventing and treating liver injury Download PDFInfo
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Abstract
本发明公开了一种大枣叶活性提取物及其在制备防治肝损伤药物或保健品中的应用。本发明首次采用大孔树脂耦合聚酰胺树脂，然后通过制备液相制备大枣叶精制提取物，较传统的分离纯化方法分离效率更高，且分离得到的活性成分含量高，生物活性强，安全性高。经实验研究表明，大枣叶提取物可显著降低CCl4、D-GalN和乙醇所致肝损伤模型小鼠血清ALT、AST活性及肝组织MDA含量，增强肝脏SOD活性，对肝损伤具有很好的防治功效。因此大枣叶及大枣叶提取物有望开发成为新一代安全有效，用于防治肝损伤的药物或保健品，同时也为大枣叶废弃资源变废为宝及枣树资源综合利用提供了途径。The invention discloses an active extract of jujube leaves and its application in preparing medicines or health care products for preventing and treating liver damage. The present invention uses macroporous resin to couple polyamide resin for the first time, and then prepares the refined extract of jujube leaves by preparing liquid phase, which has higher separation efficiency than traditional separation and purification methods, and the separated active ingredients have high content, strong biological activity and safety high sex. Experimental studies have shown that jujube leaf extract can significantly reduce the serum ALT, AST activity and liver tissue MDA content of liver injury model mice caused by CCl 4 , D-GalN and ethanol, and enhance liver SOD activity, which has a good effect on liver injury. preventive efficacy. Therefore, jujube leaves and jujube leaf extracts are expected to be developed into a new generation of safe and effective drugs or health products for preventing and treating liver damage, and also provide a way for jujube leaf waste resources to be turned into treasures and jujube tree resources to be comprehensively utilized .
Description
Technical field
The present invention relates to extractive technique and the application thereof of a Plant Extracts, be specifically related to extractive technique and the application in preparation control liver injury medicament or health product thereof of Chinese date leaf extract.
Background technology
Liver is one of most important internal organs of body, and hepatic disease is comparatively common clinically disease.Therefore, hepatic disease is one of object of medical science primary study always.Hepatic injury is the total pathological state a kind of with conspicuous characteristics of multiple hepatic disease, and human health in serious threat.Various harmful factors such as medicine, virus, ethanol, biology etc. may cause liver function that in various degree infringement is arranged, thereby make detoxifcation, the excretory function of liver and stock with regeneration capacity and reduce, hepatic blood flow reduces, metabolic burden increases the weight of, thereby environment disturbance in occuring, hepatic necrosis and apoptosis, and then cause hepatic injury.The whole world has billions of people once or just standing the misery of hepatic injury.The long-term existence of hepatic injury tends to cause hepatic fibrosis, is and then brings out liver cirrhosis, liver failure, the important initiating agent of hepatocarcinoma even.Therefore prevention and treatment hepatocyte injury are one of important steps of liver disease clinically, are the bases that suppresses the Occurrence and development of diseases such as hepatic fibrosis, hepatic necrosis, liver cirrhosis and hepatocarcinoma.Therefore study hepatic injury control medicine and have important clinical significance.Present clinical hepatic commonly used, or because expensive, or because using inconvenience, or having larger side effect, use is restricted.Therefore, further seek the striving direction that the active drug that is used for prevention and treatment hepatic injury remains current pharmacology worker.
The dried leaves of Fructus Jujubae leaf system Rhamnaceae jujube Fructus Jujubae (Ziziphus jujuba Mill.).The book on Chinese herbal medicine Chinese date leaf can be used for treating the diseases such as fever of children, furuncle, Re Rush, rotten foot, burn and scald as medicinal.Modern study about Chinese date leaf is less, and except using as the tea-drinking development of raw materials on a small quantity, how less being utilized abandons as waste resource.Have not yet to see the technology for preparing activity extract take Chinese date leaf as raw material, also have no and be applied to prevent and treat the medicine of hepatic injury or the report of health food take Chinese date leaf as the chief component component.
Summary of the invention
Goal of the invention: the objective of the invention is in order to overcome the deficiencies in the prior art, on the basis of the original purposes of Chinese date leaf, develop its new clinical application, the application of Chinese date leaf in the control hepatic injury is provided, experimental result shows, it has the effect of good control hepatic injury.Another object of the present invention is to disclose a kind of preparation method of Chinese date leaf extract.
Technical scheme: in order to realize above purpose, the technical scheme that the present invention takes is:
A kind of Chinese date leaf extract, it is to prepare by following steps:
(1) get Chinese date leaf selection, clean, put into extraction pot after drying, add 50%～95% ethanol of 6～12 times of amounts, reflux 1～3 time is filtered, merge extractive liquid,, is concentrated into without after the alcohol flavor, the ethanol extract that gets Chinese date leaf is standby;
(2) get the Chinese date leaf ethanol extract that step (1) obtains, add 1～5 times of water gaging, upper macroporous resin column, first water and concentration are 10% ethanol elution respectively, be 60%～95% ethanol elution again with concentration, collect 60%～95% ethanol elution, concentrated, drying under reduced pressure obtains Chinese date leaf active site crude extract, and is standby;
(3) get polyamide column on the Chinese date leaf active site crude extract dry method that step (2) obtains, first distinguish water and 20% ethanol elution, be 70%～95% ethanol elution with concentration again, and collect 70%～95% ethanol elution, concentrated, obtain the refining extract of Chinese date leaf active site through drying under reduced pressure;
(4) get step (3) and obtain Chinese date leaf active site extract and adopt the further enrichment of preparation liquid phase to obtain refining Fructus Jujubae extract, after testing, total triterpene and general flavone content〉80%.
As preferred version, above-described Chinese date leaf extract, extraction conditions is that to add 10 times of amount concentration be 80% ethanol in step (1), is heated to 80 ℃ and continues to reflux 2 hours.The method can guarantee to extract and reach enough large concentration difference between solution and medical material, increase the dissolution rate of target component, because concentration of alcohol is too low, triterpenic acid active component yield in Chinese date leaf is too low, the flavonoid glycoside composition rate of transform in the too high Chinese date leaf of concentration of alcohol is too low, and the impurity rates of transform such as fatty acid, chlorophyll increase.Therefore the present invention according to the character of active component, determines concentration and the consumption of ethanol, and the extract target component dissolution rate that obtains is high, and activity is stronger.
As preferred version, above-described Chinese date leaf extract, the macroporous adsorbent resin described in step (2) is nonpolar macroporous adsorption resin.As more preferably scheme, wherein the nonpolar macroporous adsorption resin described in step (2) is D101 type macroporous adsorbent resin, and collecting concentration is 90% ethanol elution; Collecting concentration in step (3) is 90% ethanol elution.the method that the present invention combines with modern times extraction separation means and Pharmacological Activity Screening is determined the best control of Chinese date leaf hepatic injury activity extract, show through lot of experiments, by nonpolar macroporous adsorbing coupled polyamide column chromatography, adopt the ethanol elution of variable concentrations, be conducive to flavonoid in Chinese date leaf and the enrichment of triterpenes active component, remove saccharide, tannin, the impurity such as chlorophyll, obtain high activity component ratio and active stronger control hepatic injury activity extract, detect in this active site triterpene acids and flavones ingredient section content through HPLC-ELSD and HPLC-DAD and can reach 70%.
As preferred version, above-described Chinese date leaf extract, the particle diameter of the polyamide described in step (3) is the 60-80 order.
As preferred version, above-described Chinese date leaf extract, the chromatographic condition of the preparation liquid phase enrichment described in step (4) is: chromatographic column is the anti-phase C18 post of Agilent, mobile phase is methanol-0.5% phosphate aqueous solution gradient elution, and the condition of gradient elution is: 0 to 35 minute, the content of methanol rose to 45% by 5%, the content of 35 to 60 minutes methanol rises to 83% by 45%, the detection wavelength is 285nm, and flow velocity is 5ml/min, and detector is diode display detector.
The application in preparation control liver injury medicament or health product of Chinese date leaf and extract thereof.
The present invention shows through lot of experiments; Chinese date leaf and extract thereof can effectively reduce liver injury model animal serum ALT, AST activity and Liver MDA; strengthen liver SOD active; show that Chinese date leaf has obvious liver protection, can be applicable to prepare medicine or the health food of preventing and treating hepatic injury.
Chinese date leaf extract of the present invention is in the medicine of preparation control hepatic injury or the application in health food, Fructus Jujubae extract and pharmaceutically acceptable carrier made the medicine of tablet, injection, capsule, granule, pill, micropill, powder, drop pill, decoction, syrup, mixture, soft extract or extractum dosage form.
When Chinese date leaf extract provided by the invention is made capsule Chinese date leaf extract and carrier lactose or corn starch mix homogeneously, granulate, the then encapsulated capsule of making.
When Chinese date leaf extract provided by the invention is made tablet, Chinese date leaf extract and lactose or corn starch, add magnesium stearate lubricant when needing, mix homogeneously, then tabletting is made tablet.
During Chinese date leaf extract granulation agent provided by the invention, Chinese date leaf extract and diluent lactose or corn starch mix homogeneously, granulate, drying, granulation agent.
When Chinese date leaf extract provided by the invention is made injection, getting the Chinese date leaf extract adds physiological saline solution then to add activated carbon, stir, 80 ℃ were heated 30 minutes, filter, regulate pH value, be filtered to clear and bright with sintered glass funnel or other filter, fill was made injection in 30 minutes 100 to 115 ℃ of sterilizations.
When Chinese date leaf extract provided by the invention is made other dosage form, can prepare by the pharmacy conventional method.
Beneficial effect: the present invention carries out new purposes exploitation on the basis of the original application of Chinese date leaf, prepare simultaneously its activity extract, compares with existing prior art to have the following advantages:
(1) the present invention mainly to produce the garbage Chinese date leaf that produces in the Fructus Jujubae process as raw material, prepares its activity extract, develops its new purposes, realization is turned waste into wealth, but promoted the value of jujube tree, realized the comprehensive utilization of jujube resources, had good economic benefits.
(2) Chinese date leaf control hepatic injury activity extract provided by the invention, experimental result shows can significantly reduce CCl
4Cause that liver injury model mice, ethanol cause the liver injury model mice, D-Gal causes liver injury model mice serum ALT, AST activity and Liver MDA; strengthen liver SOD active; show this activity extract not only to chemical liver injury, drink and cause hepatic injury and have obvious liver protection for a long time, simultaneously hepatic injury due to viral hepatitis is also had protective effect.Therefore Chinese date leaf and extract thereof are expected to exploitation to become a new generation safe and effective, are used for medicine or the health product of control polytype hepatic injury, also turn waste into wealth for the Chinese date leaf waste resource simultaneously and the jujube resources comprehensive utilization provides approach.
(3) preparation method provided by the invention is workable, especially the present invention adopts macroporous resin and polyamide coupling first in separation process, and the further enriching and purifying of employing preparation liquid phase, the extraction separation efficiency is high, separates the Chinese date leaf extract active component content that obtains high, and antihepatitis activity is stronger, untoward reaction is lower, and consuming little energy in preparation technology, production cost is low, environmentally safe.
The specific embodiment
Further illustrate the present invention below in conjunction with specific embodiment, should understand these embodiment only is used for explanation the present invention and is not used in and limits the scope of the invention, after having read the present invention, those skilled in the art all fall within the application's claims limited range to the modification of the various equivalent form of values of the present invention.
Embodiment 1
Chinese date leaf control hepatic injury activity extract, it prepares by the following method:
(1) get Chinese date leaf selection, clean, take 10kg after drying, put into extraction pot, add 80% ethanol of 10 times of amounts, reflux 3 times, each 2h filters, merge extractive liquid,, is concentrated into without after the alcohol flavor, the ethanol extract (2L) that gets Chinese date leaf is standby;
(2) get the Chinese date leaf ethanol extract that step (1) obtains, add 2 times of water gagings, adding activated diameter after mixing is 50 centimetres, and in the D101 macroporous adsorption resin chromatography post of high 1.5 meters, preadsorption is after 12 hours, press respectively the speed eluting of 2BV/h with water, 10% ethanol and 90% ethanol, elution volume is 50L, collects 90% ethanol elution, is concentrated into without the alcohol flavor, obtain Chinese date leaf active site crude extract 1L, standby;
(3) get that on the Chinese date leaf active site crude extract dry method that step (2) obtains, activated diameter is 20 centimetres, the polyamide column of high 1.5 meters (60～80 order), first use respectively water and 20% ethanol elution of 20L, be 90% ethanol elution with concentration again, and collect 90% ethanol elution, concentrated, obtain the refining extract 610g of Chinese date leaf active site through drying under reduced pressure;
(4) get step (3) and obtain Chinese date leaf active site extract and adopt the further enrichment of preparation liquid phase to obtain refining Fructus Jujubae extract, after testing, total triterpene and general flavone content〉80%.The preparative liquid chromatography condition is: chromatographic column is the anti-phase C18 post of Agilent, mobile phase is methanol-0.5% phosphate aqueous solution gradient elution, the condition of gradient elution is: 0 to 35 minute, the content of methanol rises to 45% by 5%, the content of 35 to 60 minutes methanol rises to 83% by 45%, the detection wavelength is 285nm, and flow velocity is 5ml/min, and detector is diode display detector.
Embodiment 2
Chinese date leaf control hepatic injury activity extract, it prepares by the following method:
(1) get Chinese date leaf selection, clean, take 10kg after drying, put into extraction pot, add 70% ethanol of 10 times of amounts, reflux 3 times, each 2h filters, merge extractive liquid,, is concentrated into without after the alcohol flavor, the ethanol extract (2.5L) that gets Chinese date leaf is standby;
(2) get the Chinese date leaf ethanol extract that step (1) obtains, add 1.5 times of water gagings, adding activated diameter after mixing is 50 centimetres, and in the AB-8 macroporous adsorption resin chromatography post of high 1.5 meters, preadsorption is after 12 hours, press respectively the speed eluting of 2BV/h with water, 10% ethanol and 85% ethanol, elution volume is 50L, collects 85% ethanol elution, is concentrated into without the alcohol flavor, obtain Chinese date leaf active site crude extract 1L, standby;
(3) get that on the Chinese date leaf active site crude extract dry method that step (2) obtains, activated diameter is 20 centimetres, the polyamide column of high 1.5 meters (60～80 order), first use respectively water and 20% ethanol elution of 20L, be 80% ethanol elution with concentration again, and collect 80% ethanol elution, concentrated, obtain the refining extract 570g of Chinese date leaf active site through drying under reduced pressure;
(4) get step (3) and obtain Chinese date leaf active site extract and adopt the further enrichment of preparation liquid phase to obtain refining Fructus Jujubae extract, after testing, total triterpene and general flavone content〉80%.The preparative liquid chromatography condition is: chromatographic column is the anti-phase C18 post of Agilent, mobile phase is methanol-0.5% phosphate aqueous solution gradient elution, the condition of gradient elution is: 0 to 35 minute, the content of methanol rises to 45% by 5%, the content of 35 to 60 minutes methanol rises to 83% by 45%, the detection wavelength is 285nm, and flow velocity is 5ml/min, and detector is diode display detector.
Embodiment 3
Chinese date leaf control hepatic injury activity extract, it prepares by the following method:
(1) get Chinese date leaf selection, clean, take 10kg after drying, put into extraction pot, add 90% ethanol of 8 times of amounts, reflux 2 times, each 2h filters, merge extractive liquid,, is concentrated into without after the alcohol flavor, the ethanol extract (1.6L) that gets Chinese date leaf is standby;
(2) get the Chinese date leaf ethanol extract that step (1) obtains, add 3 times of water gagings, adding activated diameter after mixing is 50 centimetres, and in the HP-20 macroporous adsorption resin chromatography post of high 1.5 meters, preadsorption is after 12 hours, press respectively the speed eluting of 2BV/h with water, 10% ethanol and 95% ethanol, elution volume is 50L, collects 95% ethanol elution, is concentrated into without the alcohol flavor, obtain Chinese date leaf active site crude extract 1L, standby;
(3) get that on the Chinese date leaf active site crude extract dry method that step (2) obtains, activated diameter is 20 centimetres, the polyamide column of high 1.5 meters (100 order), first use respectively water and 20% ethanol elution of 20L, be 95% ethanol elution with concentration again, and collect 80% ethanol elution, concentrated, obtain the refining extract 6050g of Chinese date leaf active site through drying under reduced pressure;
(4) get step (3) and obtain Chinese date leaf active site extract and adopt the further enrichment of preparation liquid phase to obtain refining Fructus Jujubae extract, after testing, total triterpene and general flavone content〉81%.The preparative liquid chromatography condition is: chromatographic column is the anti-phase C18 post of Agilent, mobile phase is methanol-0.5% phosphate aqueous solution gradient elution, the condition of gradient elution is: 0 to 35 minute, the content of methanol rises to 45% by 5%, the content of 35 to 60 minutes methanol rises to 83% by 45%, the detection wavelength is 285nm, and flow velocity is 5ml/min, and detector is diode display detector.
Embodiment 4
The experimentation of Chinese date leaf extract anti-liver injury
One, experiment material and medicine
1. medicine and reagent
AST, ALT, MDA, SOD test kit and Coomassie brilliant blue protein reagent box all are purchased from Nanjing and build up bio-engineering research institute; Carbon tetrachloride (CCl
4, analytical pure, Shanghai Ling Feng chemical reagent company limited), be mixed with 0.1% peanut oil solution during use with Oleum Arachidis hypogaeae semen; Galactosaminide (D-GalN, Sigma company), the distilled water dissolved dilution becomes 70mg/mL solution before use; Bifendate Tablet (Jiangsu Pengyao Pharmaceutical Co., Ltd.) faces the used time to be ground into fine powder and to add normal saline and make suspension.
2. laboratory animal
A cleaning level male ICR mouse, body weight 18～22g is provided by Zhejiang Province's Experimental Animal Center, quality certification SCXK(Zhejiang) 2008-0033.
3. experimental apparatus
Enzyme linked immunological instrument (U.S. Bio-Tek company); UV-2000 type ultraviolet spectrophotometer (Beijing Lai Baitaike instrument company limited); BT125 type electronic balance (Sai Duolisi scientific instrument company limited); KQ-250E type ultrasonic cleaner (Kunshan standing grain wound ultrasonic instrument company limited); AnkeGL-16GII type centrifuge (Anting Scientific Instrument Factory, Shanghai).
4. tested medicine and processing method
Get respectively the Chinese date leaf extract that embodiment 1～3 prepares, thin up is made desired concn; Positive drug is bifendate, is mixed with desired concn with the distilled water dissolving before administration.
Two, experimental technique
1. to CCl
4Cause the impact of acute liver
Get 60 mices, adaptability is divided into 6 groups at random by body weight after feeding for 1 week, 10 every group, is respectively Normal group, CCl
4To the liver injury model group, the Chinese date leaf extract low dose group (0.25g/kg/d) that embodiment 2 prepares, middle dosage group (0.5g/kg/d) and high dose group (1.0g/kg/d), bifendate group (150mg/kg/d).Matched group and model group give commensurability normal saline, the continuous gavage 10d of administration group, and 1h after the last administration, except normal group ip0.9%NaCl10mL/kg, all the other respectively organize equal ip0.1%CCl
4Peanut oil solution 10mL/kg.After 16h, all mices are adopted the blood sampling of eye socket rear vein beard, separation of serum, the activity of mensuration serum alt and AST.Put to death mice after getting blood, get immediately the part liver, be prepared into 10% liver homogenate with normal saline, measure MDA content and SOD vigor according to the test kit explanation.
2. D-GalN is caused the impact of acute liver
Get 60 mices, after adaptability fed for 1 week, be divided at random 6 groups by body weight, every group 10, be respectively Normal group, D-GalN causes the liver injury model group, the Chinese date leaf extract low dose group (0.25g/kg/d) that embodiment 1 prepares, middle dosage group (0.5g/kg/d) and high dose group (1.0g/kg/d), bifendate group (150mg/kg/d).Matched group and model group give commensurability normal saline, the continuous gavage 10d of administration group, and 1h after the last administration, except normal group ip0.9%NaCl10mL/kg, all the other respectively organize equal ipD-GalN700mg/kg.After 16h, all mices are adopted the blood sampling of eye socket rear vein beard, separation of serum, the activity of mensuration serum alt and AST.Put to death mice after getting blood, get immediately the part liver, be prepared into 10% liver homogenate with normal saline, measure MDA content and SOD vigor according to the test kit explanation.
3. ethanol is caused the impact of mouse liver injury
Get 60 mices, after adaptability fed for 1 week, be divided at random 6 groups by body weight, every group 10, be respectively Normal group, ethanol causes the liver injury model group, the Chinese date leaf extract low dose group (0.25g/kg/d) that embodiment 3 prepares, middle dosage group (0.5g/kg/d) and high dose group (1.0g/kg/d), bifendate group (150mg/kg/d).Except Normal group (with isopyknic distilled water gavage), all the other each groups all give 50% ethanol by 7mL/kg/ gavage, and 2 times/d, midfeather 6h, 15d continuously.From modeling the 1st day, every day, gastric infusion was 1 time.Normal group and model control group gavage isopyknic distilled water, and all the other organize respectively respectively that gavage gives corresponding Chinese date leaf extract and bifendate.After last 1 gavage 6h, all mices are adopted the blood sampling of eye socket rear vein beard, separation of serum, the activity of mensuration serum alt and AST.Put to death mice after getting blood, get immediately the part liver, be prepared into 10% liver homogenate with normal saline, measure MDA content and SOD vigor according to the test kit explanation.
4. statistical procedures
All experimental datas all adopt the SPSS11.5 statistical processing software to carry out statistical procedures, result with
Variance analysis is relatively adopted in expression between group.
Three, experimental result
1. the Chinese date leaf extract is to CCl
4Cause the impact of acute liver
CCl
4Liver injury model group and normal group compare, and mice serum ALT, AST and hepatic tissue MDA level obviously raise (P＜0.01), the active obviously reduction (P＜0.01) of SOD; Each dosage group of the Chinese date leaf extract that Chinese date leaf, embodiment 2 prepare, bifendate group and model group make hepatic injury mice serum ALT, AST reduce (P＜0.01) more to some extent; Chinese date leaf extract low dose group has the hepatic tissue of reduction MDA level, the trend of rising hepatic tissue SOD level, but there was no significant difference (P〉0.05); Chinese date leaf extracts object height, middle dosage group and bifendate group and model group makes hepatic injury murine liver tissue MDA level reduce (P＜0.05) more to some extent, SOD level rising (P＜0.05).Concrete experimental result sees Table 1.
Table 1 Chinese date leaf extract is to CCl
4Cause the impact (mean ± SD, n=10) of acute hepatic injury mice Serum ALT, AST and liver SOD, MDA
Annotate: compare with model group,
##P＜0.01,
#P＜0.05.
2. the Chinese date leaf extract causes the impact of acute liver on D-GalN
D-GalN liver injury model group and normal group compare, and mice serum ALT, AST and hepatic tissue MDA level obviously raise (P＜0.01), the active obviously reduction (P＜0.01) of SOD; Chinese date leaf, each dosage group of the Chinese date leaf extract that embodiment 1 prepares, bifendate group and model group make hepatic injury mice serum ALT, AST reduce (P＜0.01) more to some extent; Each dosage group of Chinese date leaf extract and bifendate group and model group make hepatic injury murine liver tissue MDA level reduce (P＜0.05) more to some extent, SOD level rising (P＜0.05).Concrete experimental result sees Table 2.
Table 2 Chinese date leaf extract causes the impact (mean ± SD, n=10) of acute hepatic injury mice Serum ALT, AST and liver SOD, MDA on D-GalN
Annotate: compare with model group,
##P＜0.01,
#P＜0.05.
3. the Chinese date leaf extract causes the impact of acute liver on ethanol
Ethanol causes the liver injury model group and normal group compares, and mice serum ALT, AST and hepatic tissue MDA level obviously raise (P＜0.01), the active obviously reduction (P＜0.01) of SOD; Chinese date leaf, each dosage group of the Chinese date leaf extract that embodiment 3 prepares, bifendate group and model group make hepatic injury mice serum ALT, AST reduce (P＜0.05) more to some extent; Each dosage group of Chinese date leaf extract and bifendate group and model group make hepatic injury murine liver tissue MDA level reduce (P＜0.05) more to some extent, SOD level rising (P＜0.05).Concrete experimental result sees Table 3.
Table 3 Chinese date leaf extract causes the impact (mean ± SD, n=10) of acute hepatic injury mice Serum ALT, AST and liver SOD, MDA on ethanol
Annotate: compare with model group,
##P＜0.01,
#P＜0.05.
CCl is adopted in this experiment
4, 3 kinds of Experimental Mice liver injury models research Chinese date leafs of D-GalN and ethanol and extract thereof hepatoprotective effect, CCl wherein
4It is the toxic agent of the chemical liver injury animal model of classics.The pathological change of the pathological change of D-GalN liver injury model and human virus's hepatitis is comparatively approaching, its intoxicating mechanism be in metabolic process with body in phosphoric acid uridine a large amount of in conjunction with forming stable uridine derivatives, cause uridnine exhaustion in body, thereby make to rely on phosphoric acid uridine and carry out the material biosynthesis blocks such as biosynthetic RNA, protein and sugar be former, cause hepatic injury even dead.In addition, D-GalN also can cause lipid peroxidation by producing free radical, causes hepatocyte injury.It is the hepar damnification disease of simulation due to long-term heavy drinking that ethanol causes liver injury model.Above experimental result shows, Chinese date leaf provided by the invention and Chinese date leaf extract thereof can significantly reduce CCl
4, liver injury model mice serum ALT, AST activity and Liver MDA due to D-GalN and ethanol; strengthen liver SOD active; and contrast and experiment shows, the Chinese date leaf extract after refining through the present invention has than the better liver protection of the Chinese date leaf of equivalent.Therefore Chinese date leaf and extract thereof are expected to exploitation to become a new generation safe and effective, are used for medicine or the health product of control hepatic injury, and compare with existing medicine, and Fructus Jujubae extract is safer, has no adverse reaction.While also turns waste into wealth for the Chinese date leaf waste resource and the jujube resources comprehensive utilization provides approach.
The above is only the preferred embodiment of the present invention; should be pointed out that for those skilled in the art, under the prerequisite that does not break away from the principle of the invention; can also make some improvements and modifications, these improvements and modifications also should be considered as protection scope of the present invention.
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| CN105232670A (en) * | 2015-11-11 | 2016-01-13 | 王书汉 | Extraction method of effective components of jujube tree leaves and obtained product |
| CN105560438A (en) * | 2015-12-01 | 2016-05-11 | 西北大学 | Method for extracting and purifying phenolic compounds from fructus jujubae honey |
| CN105687367A (en) * | 2016-01-25 | 2016-06-22 | 西北大学 | Red jujube leaf standard extract, as well as preparation method and application thereof |
| CN105884842A (en) * | 2016-01-28 | 2016-08-24 | 塔里木大学 | Quercetin-3-O-alpha-L-arabinose-(1->2)-alpha-L-rhamnoside prepared from red date leaves and application thereof |
| CN110101731A (en) * | 2019-06-14 | 2019-08-09 | 南京中医药大学 | Chrysanthemum cauline leaf activity extract and the preparation method and application thereof with prevention and treatment eye disease |
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| CN105560438B (en) * | 2015-12-01 | 2019-10-25 | 西北大学 | A method for extracting and purifying phenolic compounds from jujube nectar |
| CN105687367A (en) * | 2016-01-25 | 2016-06-22 | 西北大学 | Red jujube leaf standard extract, as well as preparation method and application thereof |
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| CN114736106A (en) * | 2022-04-28 | 2022-07-12 | 江西中医药大学 | Lignan dimer compound and preparation method and application thereof |
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