Automatically tackling blockage method and system in blood cell analysis equipment
Technical field
The present invention relates to a kind of blood cell analysis equipment, especially relate to a kind of method that is applied to flow chamber and specimen needle plug-hole fault automatic identification in blood cell analysis equipment, Intelligent treatment, the output of unimpeded result, and corresponding automatically tackling blockage system.
Background technology
In blood cell analysis equipment, by sample syringe and sheath liquid syringe, promote to do to form sheath fluid, if generation flow chamber or specimen needle plug-hole can cause following result:
1, after flow chamber is blocked, the DIFF channel function can't be realized, causes blood cell analysis equipment normally to use.
2, adopt the sheath liquid syringe to push away under the scheme of sheath fluid, stop up if flow chamber occurs, the flow chamber internal pressure increases suddenly, can cause pipeline to collapse de-.
If 3 plug-hole judgements and unimpeded are processed not in time, stop up material further deeply, will further increase the unimpeded difficulty, thereby affect the client, normally use blood cell analysis equipment.
At present, for cellanalyzer flow chamber and specimen needle plug-hole fault, the anti-blocking blockage-eliminating device generally adopted mostly is to be increased filtrator and takes pressure switch to report to the police in the sheath fluid loop, and takes syringe resorption mode to carry out unimpeded.This kind scheme can not tackle the problem at its root, and this scheme has the following disadvantages: 1, sample preparation pipeline can't add filtrator; 2, resorption is subjected to negative pressure restriction dynamics inadequate, and directly resorption can cause air pocket, further reduces the resorption dynamics; If the plug-hole 3, occurred when walking sample directly resorption has sample to enter the risk of syringe; 4, whether resorption is removed tamper and can't be determined; 5 and most cellanalyzer sheath fluid entrance in side, cause realizing that flow chamber is emptying, bring repeatedly flow chamber stifled.
At present, solve the blockage-eliminating device of the plug-hole fault employing of Hematometer flow chamber, specimen needle, be mostly to increase in the sheath fluid loop filtrator and take pressure switch to report to the police, syringe resorption mode is taked in unimpeded.But this solution has the following disadvantages: 1, resorption is subjected to negative pressure restriction dynamics inadequate, and directly resorption can cause air pocket, further reduces the resorption dynamics; 2, specimen needle occur plug-hole the time, if direct resorption blood sample may enter syringe; 3, whether resorption is removed tamper and can't be determined; 4, most cellanalyzer sheath fluid entrances cause in side realizing that flow chamber is emptying, bring repeatedly flow chamber stifled.5, sample preparation pipeline can't add filtrator.
Summary of the invention
The present invention proposes automatically tackling blockage method and system in a kind of blood cell analysis equipment, by automatic plug-hole judgement, starts the unimpeded sequential, and has higher unimpeded success ratio.
The present invention adopts following technical scheme to realize: a kind of automatically tackling blockage method in blood cell analysis equipment, and it comprises step:
Force value in signal piping, compare this force value and default pressure threshold in real time, judges whether to occur plug-hole;
If the generation plug-hole, distinguish flow chamber generation plug-hole or specimen needle generation plug-hole according to current operation sequential;
Start the flow chamber unimpeded or to the specimen needle unimpeded respectively of flow chamber recoil unimpeded sequential or specimen needle recoil unimpeded sequential.
Wherein, described flow chamber recoil unimpeded sequential comprises step:
By the sheath liquid syringe, discharged the outlet end of sheath fluid recoil flow chamber, the tamper that is blocked in the outlet end of flow chamber is rushed to flow chamber;
By the liquid in sheath liquid syringe resorption flow chamber, with by the tamper resorption to the sheath liquid syringe to valve in 2 sections pipelines;
Tamper is drained into to the waste liquid apotheca, by the waste liquid apotheca, tamper is discharged.
Wherein, described specimen needle recoil unimpeded sequential comprises step:
By the sheath liquid syringe, discharge sheath fluid to flow chamber, the outlet end of sheath fluid build-up pressure the specimen needle that recoils in flow chamber, recoil out from the liquid feeding end of specimen needle by tamper;
Tamper is drained into to the waste liquid apotheca, by the waste liquid apotheca, tamper is discharged.
Wherein, each flow chamber recoil unimpeded sequential or each specimen needle recoil unimpeded sequential include step: the 3rd controlled valve that intermittence is opened on pipeline carries out release, makes the force value of pipeline be less than described pressure threshold.
Wherein, after the chamber unimpeded of a plurality of flow chamber recoil unimpeded sequential flow, or during through a plurality of specimen needle recoil unimpeded after the unimpeded of ordered pair specimen needle, among ensuing flow chamber recoil unimpeded sequential or specimen needle recoil unimpeded sequential, do not open the 3rd controlled valve and carry out release, judge whether the force value in pipeline is greater than pressure threshold, if not, show the unimpeded success.
Automatically tackling blockage system in a kind of blood cell analysis equipment, wherein, blood cell analysis equipment comprises at least: the sheath liquid syringe; Flow chamber; Outlet end is arranged on the sampling needle among flow chamber; Described automatically tackling blockage system comprises at least:
Be arranged on the pressure transducer on the outlet end pipeline of sheath liquid syringe, monitor in real time for the force value to pipeline;
Be connected to the first controlled valve between the liquid feeding end of the outlet end of sheath liquid syringe and sampling needle;
The outlet end of sheath liquid syringe connects first of the second controlled valve by sebific duct and controls mouth, and the second control mouth of the second controlled valve and the 3rd control mouth are connected respectively liquid feeding end and the outlet end of flow chamber, and the first control of the second controlled valve mouthful is optionally controlled a mouth conducting with the second control mouth or the 3rd;
Be connected to the 3rd controlled valve between the liquid feeding end of the outlet end of flow chamber and waste liquid apotheca;
Be connected to the 4th controlled valve between the liquid feeding end of the liquid feeding end of sampling needle and waste liquid apotheca;
By AD sample circuit Bonding pressure sensor and connect first, second, third and the controller of the 4th controlled valve, for this force value and default pressure threshold are compared, judge whether to occur plug-hole, if generation plug-hole, according to current operation sequential, distinguish flow chamber generation plug-hole or specimen needle generation plug-hole, and control and start the flow chamber unimpeded or to the specimen needle unimpeded respectively of flow chamber recoil unimpeded sequential or specimen needle recoil unimpeded sequential.
Wherein, described flow chamber recoil unimpeded sequential comprises:
Controller is controlled first of the second controlled valve and is controlled conducting between mouth and the second control mouth, and controlling the 3rd controlled valve closes, promote the sheath liquid syringe sheath fluid is recoiled to the outlet end of flow chamber through the second controlled valve, the tamper of the outlet end of flow chamber is recoiled to the inside of flow chamber;
Control first of the second controlled valve and control mouthful with the 3rd and control conducting between mouth, the sheath fluid that by the sheath liquid syringe, flow chamber is contained to tamper through the second controlled valve resorption to the sheath liquid syringe;
The first control mouth and second of controlling the second controlled valve is controlled conducting between mouth, control the 3rd controlled valve is opened, and makes the interior liquid of sheath liquid syringe drain into the waste liquid apotheca.
Wherein, described specimen needle recoil unimpeded sequential comprises:
Control first of the second controlled valve and control conducting between mouth and the 3rd control mouth, promote the sheath liquid syringe and make sheath fluid carry out flow chamber by the second controlled valve, flow chamber produces certain pressure makes the tamper of the outlet end of specimen needle recoil out from the liquid feeding end of specimen needle;
The 4th controlled valve is opened in control, makes tamper drain into the waste liquid apotheca by the 4th controlled valve.
Wherein, described specimen needle recoil unimpeded sequential also comprises: the control intermittence is opened the 3rd controlled valve and is carried out release, makes the force value of pipeline be less than described pressure threshold.
Compared with prior art, the present invention has following beneficial effect:
1, adopts pressure sensor Real-Time Monitoring, change by the AD sample circuit, finally by controller, implement to judge whether pressure is greater than threshold value, thereby whether intelligent decision plug-hole occurs.
2, after controller judges plug-hole, stop immediately current operation sequential, avoid pressure further rise other faults that cause and the other problems brought, such as having prevented that collapsing of pipeline is de-.
3, unimpeded process is held the flushing direction must take flow chamber, specimen needle recoil unimpeded mode by the past larger direction of pipe diameter of the less direction of pipe diameter, has improved the unimpeded success ratio.
4, from sending the plug-hole failure message, to unloading, be pressed onto unimpeded to stopping current operation sequential, finally enter the measurement standby condition, whole flow process is all the robotization redirect, realizes the robotization fault handling.
5,, after unimpeded has been moved, by building to press whether successfully to do the intelligent decision unimpeded, thereby accurately judge the unimpeded result.
6, by sheath liquid syringe resorption plug-hole material and by it, discharge with thorough unimpeded, avoid the plug-hole fault repeat occur again.
The accompanying drawing explanation
Fig. 1 is the part-structure schematic diagram of blood cell analysis equipment of the present invention;
Fig. 2 is the schematic diagram of control circuit of the present invention.
Embodiment
The present invention proposes a kind of blood cell analysis equipment, starts unimpeded by automatic plug-hole judgement, has higher unimpeded success ratio.
In conjunction with illustrated in figures 1 and 2, blood cell analysis equipment comprises at least: sheath liquid syringe 11; Be arranged on the pressure transducer on sheath liquid syringe 11 outlet end pipelines, by pressure transducer, the pressure of sheath liquid syringe 11 outlet ends monitored in real time; The outlet end of sheath liquid syringe 11 connects the first controlled valve SV1 by sebific duct, and the first controlled valve SV1 connects sampling needle 13 by sebific duct, and the outlet end of sampling needle 13 (pin mouth) is arranged among flow chamber 12; The outlet end of sheath liquid syringe 11 connects first of the second controlled valve SV2 by sebific duct and controls mouth 21, and the second control of the second controlled valve SV2 mouthful the 22 and the 3rd control mouth 23 is connected respectively liquid feeding end and the outlet end of flow chamber 12 by sebific duct, and the first control mouth 21 of the second controlled valve SV2 is optionally controlled mouth the 22 or the 3rd control mouth 23 conductings with second; The outlet end of flow chamber 12 connects the 3rd controlled valve SV3 by sebific duct, and the 3rd controlled valve SV3 connects the liquid feeding end of waste liquid apotheca 14 by sebific duct; And the liquid feeding end of sampling needle 13 connects the 4th controlled valve SV4 by sebific duct, by sebific duct, connect the liquid feeding end of waste liquid apotheca 14 by the 4th controlled valve SV4.
In conjunction with shown in Figure 2, pressure transducer serial connection AD sample circuit connects controller, by the AD sample circuit, the testing result of pressure transducer sampled and carry out exporting to controller after analog to digital (A-D) conversion, controller obtains the force value that the current pressure sensor detects, by controller by the threshold value of this force value and prediction relatively, thereby can judge the current plug-hole that whether occurs, cause hypertonia.In addition, the first controlled valve SV1, the second controlled valve SV2 and the 3rd controlled valve SV3 all are connected the control port of controller, by controller according to the state of controlling control signal corresponding to sequential output and control each controlled valve.
Blood cell analysis equipment is when working, and promotion sheath liquid syringe 11, make the sheath fluid in sheath liquid syringe 11 discharge from the outlet end of sheath liquid syringe 11, and enters flow chamber 12 through the first control mouthful the 21 and the 3rd control mouth 23 of the second controlled valve SV2; And the blood sample of controlling sampling needle 13 is expelled to flow chamber 12 from sampling needle 13, blood sample and sheath fluid form sheath stream in flow chamber 12, (laser scattering method refers to laser and makes light source to use laser scattering method to carry out five classification to leucocyte, beyond the incident light direction, borrow the method that detects scattered light intensity, frequency displacement and angle dependence thereof etc. and obtain the information such as particle weight, size, distribution and aggregated structure).
Due to pressure transducer, be arranged on the outlet end pipeline place of sheath liquid syringe 11, the every 100ms of pressure transducer once samples, pressure signal is converted into to voltage signal, by the AD sample circuit, voltage signal is converted into to digital pressure signal again, by controller, this digital pressure signal and default threshold value are compared: can cause pressure rise if plug-hole occurs, make the digital pressure signal detected surpass not plug-hole normal condition (being threshold value), plug-hole occurs in judgement.
Such as, when sheath liquid syringe 11 normally pushed away sheath fluid, the pressure of its outlet end should be in 160kPa, and the maximum working pressure (MOP) of considering each controlled valve SV1, SV2, SV3 and SV4 is 250kPa, sebific duct collapses de-pressure in the 250kPa left and right, therefore the pressure threshold setting range that will report to the police is at 180kPa.
Wherein, due to controller by sequential control sheath liquid syringe 11 in different time sections through the second controlled valve SV2 flow chamber 12 and through the first controlled valve SV1, specimen needle 13 is pushed away to liquid, so with pressure transducer, carry out pressure monitoring pushing away 13 liang of time periods of flow chamber 12 and specimen needle, plug-holes occur in flow chamber 12 or plug-holes occur specimen needle 13 thereby distinguish.Such as, current sequential section is to control sheath liquid syringe 11 to push away liquid to flow chamber 12, namely right of the second controlled valve SV2 is communicated with (i.e. the first control mouthful the 21 and the 3rd control mouth 23 is communicated with), the 3rd controlled valve SV3 opens, make the sheath fluid flow path be: sheath liquid syringe 11 through the second controlled valve SV2 to flow chamber 12, by the 3rd controlled valve SV3, flow to waste liquid apotheca 14 again, if plug-hole detected at this course pressure sensor, be judged as the outlet end generation plug-hole of flow chamber 12.
If controller detects when plug-hole occurs, controller stops current operation sequential immediately, de-to avoid the further rising of pressure to cause each sebific duct to collapse, then automatically start the release sequential, shed the residual high pressure in pipeline, after release, robotization starts corresponding unimpeded sequential (flow chamber recoil unimpeded sequential or specimen needle recoil unimpeded sequential).
Wherein, flow chamber 12 recoil unimpeded sequential comprise step:
At first, by sheath liquid syringe 11, discharged the outlet end of sheath fluid recoil flow chamber 12, rush to flow chamber 12 with the tamper that will be blocked in flow chamber 12 outlet ends.Specifically, by controller, control first of the second controlled valve SV2 and control conducting between mouthful the 21 and second control mouth 22, and by controller, controlling the 3rd controlled valve SV3 closes, promote sheath liquid syringe 11, sheath fluid in sheath liquid syringe 11 is recoiled to the outlet end of flow chamber 12 through the second controlled valve SV2, the tamper of the outlet end of flow chamber 12 is recoiled to the inside of flow chamber 12.
Secondly, by the liquid in sheath liquid syringe 11 resorption flow chambers 12, with by the tamper resorption to pipeline between sheath liquid syringe 11 and SV2.Specifically, by controller, control first of the second controlled valve SV2 and control conducting between mouthful the 21 and the 3rd control mouth 23, and make sheath liquid syringe 11 be operated in the resorption pattern, the sheath fluid that by sheath liquid syringe 11, flow chamber 12 is contained to tamper through the second controlled valve SV2 resorption to pipeline between sheath liquid syringe 11 and SV2.
Again, tamper is drained into to waste liquid apotheca 14, by waste liquid apotheca 14, tamper is discharged.Specifically, by conducting between the first control mouthful the 21 and second control mouth 22 of controller control the second controlled valve SV2, control the 3rd controlled valve SV3, opened, pushing sheath liquid syringe 11, make the liquid in it by the second controlled valve SV2, the 3rd controlled valve SV3, drain into waste liquid apotheca 14 successively, finally by the other waste drains pump arranged of waste liquid apotheca 14, discharge blood cell analysis equipment.
Finally, by controller, control each controlled valve SV1, SV2, SV3 and SV4 and be initialized to original state.
In addition, specimen needle 13 recoil unimpeded sequential comprise step:
At first, use sheath fluid to rinse the outlet end (pin mouth) of specimen needle 13.Specifically, by controller, control first of the second controlled valve SV2 and control conducting between mouthful the 21 and the 3rd control mouth 23, promote sheath liquid syringe 11, make the sheath fluid in sheath fluid injection 11 carry out flow chamber 12 by the second controlled valve SV2; And by controller, control intermittence and open the 3rd controlled valve SV3, the interior unimpeded hypertonia of restriction flow chamber 12 produces pipeline and collapses de-.By flow chamber 12, producing the tamper that certain pressure makes to be blocked in the outlet end of specimen needle 13 recoils out from the liquid feeding end of specimen needle 13.
Secondly, control and open the 4th controlled valve SV4 by controller, make tamper drain into waste liquid apotheca 14 by the 4th controlled valve SV4, finally by the waste drains pump arranged on waste liquid apotheca 14, discharge blood cell analysis equipment.
Finally, by controller, control each controlled valve SV1, SV2, SV3 and SV4 and be initialized to original state.
In addition, unimpeded and pressure alarm in the present invention, have been coordinated.During sort run, pressure is greater than 180KPA and reports to the police immediately, has prevented that pipeline from collapsing de-when carrying out; Still may be serious because of plug-hole in the unimpeded process, cause hypertonia to cause that pipeline collapses de-, less than better unimpeded effect, larger pressure exists again pipeline to collapse de-hidden danger to less pressure again simultaneously.For coordinating this contradiction, in the unimpeded process in recoil is built and is pressed work, in flow chamber 12 recoil unimpeded processes and in specimen needle 13 recoil unimpeded processes, all intermittence is opened the 3rd controlled valve SV3 release: unimpeded does not become pressure to continue to rise, and while rising to about 150KPA, opens three controlled valve SV3 releases, complete again again the building of release pressed recoil, through a plurality of circulations, obtain efficient unimpeded success ratio, also avoided collapsing of pipeline de-; If also considered simultaneously, recoiling through occasion, whether how the unimpeded success is differentiated, and has taked to build again pressure, does not open three controlled valve SV3 releases, if the unimpeded success now can not produce pressure and continue to rise, also just can not produce pressure transducer and report to the police; If now still the plug-hole pressure transducer is reported to the police, the prompting plug-hole still exists, and fault is not still eliminated.
Need special instruction: flow chamber 12 unimpeded sequential and specimen needle 3 unimpeded sequential be all direction that the direction little from aperture is large toward aperture (wherein, the aperture of liquid feeding end that the aperture of the liquid feeding end of flow chamber 12 is greater than aperture, the specimen needle 3 of outlet end also is greater than the aperture of outlet end) recoil, thus obtain better unimpeded effect.Initialization is carried out to each parts in flow chamber unimpeded and specimen needle unimpeded sequential end, allows blood cell analysis equipment reenter the overall measurement standby condition, whole process a series of actions automation process of sampling fully.
The foregoing is only preferred embodiment of the present invention, not in order to limit the present invention, all any modifications of doing within the spirit and principles in the present invention, be equal to and replace and improvement etc., within all should being included in protection scope of the present invention.