CN103081954A - Alcoholic solution preparation of wartwort secondary metabolism product and preparation method thereof - Google Patents

Abstract

The invention discloses an ethanol solution plant insecticide preparation of lacquer secondary metabolites and a preparation method thereof. The method of making the varnish into a solution preparation of the present invention is as follows: dry the whole varnish and crush it to 30-100 mesh; Take for 6-72 hours, stir intermittently or continuously throughout the whole process, and filter to obtain the extract; add 2-5 times the weight of ethanol to the filter residue, leaching at 20-70°C for 6-48 hours, stir intermittently or throughout the process Continue to stir, filter, repeat the leaching and filtering process 1-3 times; combine the extracts, evaporate the extracts under reduced pressure to obtain a concentrated solution with 5-20% solids and recover ethanol; add 0.002-0.15 times the concentrated solution respectively Cosolvent, stabilizer, synergist and emulsifier by weight; stirred for 0.2-3 hours, filtered, and alcohol solution preparation of secondary metabolites of Deze paint.

Description

Alcoholic solution preparation of secondary metabolites of varnish and its preparation method

technical field

The invention relates to a method for extracting secondary metabolites from Euphorbia helioscopia L., an herb of the genus Euphorbia ( Euphorbiaceae ), and preparing an agricultural insecticide with the extract.

Background technique

As people pay more and more attention to the environment and food safety, as well as the development of pesticide resistance of agricultural diseases, insects, and weeds, people pay more attention to the research of finding new pesticides from natural products. The research on finding pesticide active ingredients from natural plants is in the ascendant. Compared with organic synthetic pesticides, botanical pesticides have the characteristics of low toxicity, easy degradation, and resistance to pests.

As a traditional Chinese medicinal material, lacquer has a long history of application in my country. According to the records in "Compendium of Materia Medica", Zeqi has the effects of diuresis, swelling, phlegm, fever, stagnation and insecticide. In modern times, it can be used clinically to treat diseases such as edema, ascites, phlegm, cough and asthma, and malignant tumors. The use of varnish to kill maggots in human excrement and urine pools to reduce the number of flies has a long history of application among the people of our country. Over the years, phytochemical researchers have discovered physiologically active secondary metabolites of different structural types including terpenes, flavonoids, organic acids, phenols, steroids, etc. from varnish, causing pharmacology, pesticides, and Wide attention of chemical science and technology workers.

Contents of the invention

The purpose of the present invention is to use pure natural Chinese herbal medicine lacquer as raw material, extract secondary metabolites and prepare agricultural insecticides with various auxiliary agents such as surfactants and synergists, and produce contact killing, stomach poisoning, Anti-feeding and other functions, so as to achieve the purpose of pollution-free control of pests.

The method for extracting the secondary metabolites of the varnish and preparing the agricultural insecticide in the present invention is as follows: pulverize the dried varnish to 30-100 mesh; Leach at 20-70°C for 6-72 hours, stir intermittently or continuously throughout the process, and filter to obtain the extract; add 2-5 times the weight of the original varnish to the filter residue with ethanol, and leach at 20-70°C for 6-48 hours , stirring intermittently or continuously throughout the whole process, and filtering. The leaching and filtering process is repeated 1-3 times; the leaching solution is combined, and the leaching solution is evaporated under reduced pressure to obtain a concentrated solution of 5-20% solids and recover ethanol; add 0.002 - 0.15 times the weight of the concentrate, co-solvent, stabilizer, synergist and emulsifier, wherein co-solvent, stabilizer and synergist can be used simultaneously or only one or two of them can be used; stir for 0.2-3 hours, Filtration, alcohol solution preparation of secondary metabolites of Deze paint.

The co-solvent of the present invention is sodium benzoate or sodium salicylate; the stabilizer is N,N-dimethylformamide; the synergist is azone; the emulsifier is one or more of Tween20, Tween40, Tween60, Tween80 Several mixtures with OP-10.

The advantages of the present invention: (1) the secondary metabolites of lacquer are easy to degrade under natural conditions, and are safe to the ecological system and food production; Pests produce multiple sites and different mechanisms, and are not easy to produce drug resistance; (3) The extraction process is stable; (4) Effective against various pests such as cabbage caterpillar, hawk moth, and paramecium, and can be used for root irrigation control Leek maggots and other underground pests.

Detailed ways

The following examples are used to further illustrate the present invention, but not limit the present invention.

Examples of extraction and preparation of solution preparations:

Grind the dried varnish to 60 mesh; add 300 grams of varnish and 1500 grams of ethanol to a stirring container, soak for 72 hours at 25 ° C, stir for 0.5 hours every 6 hours, and filter to obtain the extract; add to the filter residue 1000 grams of ethanol, leaching at 25°C for 48 hours, stirring every 3 hours for 0.5 hours, filtering, repeating the leaching and filtering process twice; combining the extracts, and evaporating the extracts under reduced pressure to obtain a concentration of 10% of the solids solution; add 0.01 times the weight of concentrated solution of sodium salicylate, 0.005 times of N,N-dimethylformamide, 0.01 times of azone, 0.03 times of Tween80, 0.05 times of OP-10, stir for 1 hour, filter, Alcoholic solution preparation of secondary metabolites of Deze paint.

Typical determination of insecticidal activity:

The alcohol solution preparation of the secondary metabolites of the varnish is diluted 300 times with water. Lay a piece of filter paper on the bottom of a clean petri dish, absorb 1ml of liquid medicine to moisten the filter paper, select healthy and undamaged individuals of the same size from the collected leek maggots and put them on the wetted filter paper, put 20 in each petri dish. The maggots were cultured under suitable conditions, shaded with a black cloth, and kept moist by adding water at an appropriate time. The activities of the maggots were observed at regular intervals. During the observation, the maggots were lightly touched with a brush, and those who did not move were regarded as dead. Record the death situation of leek maggot, calculate the death rate of leek maggot 60 hours to be 85%.

Claims (4)

1. a lacquer secondary metabolite ethanol solution plant insecticide is characterized in that: taking Chinese herbal medicine as raw material, extracting with ethanol and forming with cosolvent, stabilizer, synergist and emulsifier, its preparation method Method: dry the whole herb of varnish and crush it to 30-100 mesh; add varnish and 4-6 times the weight of ethanol in a stirring container, soak at 20-70°C for 6-72 hours, stir intermittently or continue the whole process Stir and filter to obtain the extract; add 2-5 times the weight of ethanol to the filter residue, and leaching at 20-70°C for 6-48 hours, stirring intermittently or continuously throughout the process, and filtering, the leaching and filtering process Repeat 2-3 times; combine the extracts, evaporate the extracts under reduced pressure to obtain a concentrated solution with 5-20% solids and recover ethanol; add 0.002-0.15 times the weight of the concentrated solution as a co-solvent, stabilizer, and synergist And emulsifier, in which cosolvent, stabilizer and synergist can be used at the same time or only one or two of them can be used; stir for 0.2-3 hours, filter, and obtain the alcohol solution preparation of the secondary metabolites of Deze paint. 2. preparation as claimed in claim 1 and preparation method thereof, it is characterized in that described solubilizer is sodium benzoate or sodium salicylate; Described stabilizer is N, N-dimethylformamide; Synergist It is azone; the emulsifier is a mixture of one or more of Tween20, Tween40, Tween60, Tween80 and OP-10. 3. preparation as claimed in claim 1 and preparation method thereof, cosolvent, stabilizer, synergist can use simultaneously or only use wherein one or both. 4. as claimed in claim 2, sodium benzoate, sodium salicylate, N,N-dimethylformamide, azone, Tween20, Tween40, Tween60, Tween80, OP-10 all have dissolution aid, stability, penetration aid, Several of multiple functions such as synergism, solubilization, emulsification, etc., are described in claim 2 as a composition of multiple components, and are not limited to the functions.