CN103070070A - Cultivation method of seedless roxburgh roses - Google Patents
Cultivation method of seedless roxburgh roses Download PDFInfo
- Publication number
- CN103070070A CN103070070A CN2013100103588A CN201310010358A CN103070070A CN 103070070 A CN103070070 A CN 103070070A CN 2013100103588 A CN2013100103588 A CN 2013100103588A CN 201310010358 A CN201310010358 A CN 201310010358A CN 103070070 A CN103070070 A CN 103070070A
- Authority
- CN
- China
- Prior art keywords
- test
- rosa roxburghii
- tube plantlet
- illumination
- humidity
- Prior art date
- Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
- Pending
Links
- 238000012364 cultivation method Methods 0.000 title claims abstract description 11
- 241000109329 Rosa xanthina Species 0.000 title abstract 4
- 235000004789 Rosa xanthina Nutrition 0.000 title abstract 4
- 241000196324 Embryophyta Species 0.000 claims abstract description 13
- 240000002547 Rosa roxburghii Species 0.000 claims description 29
- 235000000640 Rosa roxburghii Nutrition 0.000 claims description 29
- 238000005286 illumination Methods 0.000 claims description 25
- 230000001954 sterilising effect Effects 0.000 claims description 18
- 238000004659 sterilization and disinfection Methods 0.000 claims description 18
- XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 18
- 239000002689 soil Substances 0.000 claims description 15
- 230000001939 inductive effect Effects 0.000 claims description 13
- 238000013138 pruning Methods 0.000 claims description 11
- 229920001817 Agar Polymers 0.000 claims description 10
- CZMRCDWAGMRECN-UGDNZRGBSA-N Sucrose Chemical compound O[C@H]1[C@H](O)[C@@H](CO)O[C@@]1(CO)O[C@@H]1[C@H](O)[C@@H](O)[C@H](O)[C@@H](CO)O1 CZMRCDWAGMRECN-UGDNZRGBSA-N 0.000 claims description 10
- 229930006000 Sucrose Natural products 0.000 claims description 10
- 239000008272 agar Substances 0.000 claims description 10
- 229910001562 pearlite Inorganic materials 0.000 claims description 10
- 229920003023 plastic Polymers 0.000 claims description 10
- 239000004033 plastic Substances 0.000 claims description 10
- 239000005720 sucrose Substances 0.000 claims description 10
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 claims description 5
- 239000003864 humus Substances 0.000 claims description 5
- 238000011534 incubation Methods 0.000 claims description 5
- 239000007788 liquid Substances 0.000 claims description 5
- 238000002054 transplantation Methods 0.000 claims description 5
- 238000005406 washing Methods 0.000 claims description 5
- 238000000034 method Methods 0.000 abstract description 8
- 239000000463 material Substances 0.000 abstract description 3
- 230000004075 alteration Effects 0.000 abstract description 2
- 238000009395 breeding Methods 0.000 abstract description 2
- 230000001488 breeding effect Effects 0.000 abstract description 2
- 241000220317 Rosa Species 0.000 abstract 1
- 230000007547 defect Effects 0.000 abstract 1
- 230000035755 proliferation Effects 0.000 abstract 1
- 239000003337 fertilizer Substances 0.000 description 12
- 235000013399 edible fruits Nutrition 0.000 description 10
- 210000003608 fece Anatomy 0.000 description 9
- 239000010871 livestock manure Substances 0.000 description 9
- 239000000126 substance Substances 0.000 description 6
- CIWBSHSKHKDKBQ-JLAZNSOCSA-N Ascorbic acid Chemical compound OC[C@H](O)[C@H]1OC(=O)C(O)=C1O CIWBSHSKHKDKBQ-JLAZNSOCSA-N 0.000 description 4
- 235000017166 Bambusa arundinacea Nutrition 0.000 description 4
- 235000017491 Bambusa tulda Nutrition 0.000 description 4
- 241001330002 Bambuseae Species 0.000 description 4
- 235000015334 Phyllostachys viridis Nutrition 0.000 description 4
- 239000011425 bamboo Substances 0.000 description 4
- 239000000203 mixture Substances 0.000 description 4
- 241000238631 Hexapoda Species 0.000 description 3
- 241000607479 Yersinia pestis Species 0.000 description 3
- 150000001875 compounds Chemical class 0.000 description 3
- 230000000694 effects Effects 0.000 description 3
- 238000005516 engineering process Methods 0.000 description 3
- 239000007943 implant Substances 0.000 description 3
- 229910000402 monopotassium phosphate Inorganic materials 0.000 description 3
- 235000019796 monopotassium phosphate Nutrition 0.000 description 3
- 239000002420 orchard Substances 0.000 description 3
- PJNZPQUBCPKICU-UHFFFAOYSA-N phosphoric acid;potassium Chemical compound [K].OP(O)(O)=O PJNZPQUBCPKICU-UHFFFAOYSA-N 0.000 description 3
- 238000002360 preparation method Methods 0.000 description 3
- 239000007921 spray Substances 0.000 description 3
- 230000001256 tonic effect Effects 0.000 description 3
- ZZZCUOFIHGPKAK-UHFFFAOYSA-N D-erythro-ascorbic acid Natural products OCC1OC(=O)C(O)=C1O ZZZCUOFIHGPKAK-UHFFFAOYSA-N 0.000 description 2
- UPYKUZBSLRQECL-UKMVMLAPSA-N Lycopene Natural products CC(=C/C=C/C=C(C)/C=C/C=C(C)/C=C/C1C(=C)CCCC1(C)C)C=CC=C(/C)C=CC2C(=C)CCCC2(C)C UPYKUZBSLRQECL-UKMVMLAPSA-N 0.000 description 2
- 229930003268 Vitamin C Natural products 0.000 description 2
- 230000036528 appetite Effects 0.000 description 2
- 235000019789 appetite Nutrition 0.000 description 2
- 235000005473 carotenes Nutrition 0.000 description 2
- PCHJSUWPFVWCPO-UHFFFAOYSA-N gold Chemical compound [Au] PCHJSUWPFVWCPO-UHFFFAOYSA-N 0.000 description 2
- 239000010931 gold Substances 0.000 description 2
- 229910052737 gold Inorganic materials 0.000 description 2
- 230000008929 regeneration Effects 0.000 description 2
- 238000011069 regeneration method Methods 0.000 description 2
- NCYCYZXNIZJOKI-UHFFFAOYSA-N vitamin A aldehyde Natural products O=CC=C(C)C=CC=C(C)C=CC1=C(C)CCCC1(C)C NCYCYZXNIZJOKI-UHFFFAOYSA-N 0.000 description 2
- 235000019154 vitamin C Nutrition 0.000 description 2
- 239000011718 vitamin C Substances 0.000 description 2
- 241000220324 Pyrus Species 0.000 description 1
- 241000220222 Rosaceae Species 0.000 description 1
- HCHKCACWOHOZIP-UHFFFAOYSA-N Zinc Chemical compound [Zn] HCHKCACWOHOZIP-UHFFFAOYSA-N 0.000 description 1
- 206010000210 abortion Diseases 0.000 description 1
- 231100000176 abortion Toxicity 0.000 description 1
- 239000002253 acid Substances 0.000 description 1
- 150000001413 amino acids Chemical class 0.000 description 1
- 238000004458 analytical method Methods 0.000 description 1
- 235000019606 astringent taste Nutrition 0.000 description 1
- 230000009286 beneficial effect Effects 0.000 description 1
- 210000001124 body fluid Anatomy 0.000 description 1
- 239000010839 body fluid Substances 0.000 description 1
- -1 carotene carotene Chemical class 0.000 description 1
- 230000009194 climbing Effects 0.000 description 1
- 230000029087 digestion Effects 0.000 description 1
- 239000004744 fabric Substances 0.000 description 1
- 229910052500 inorganic mineral Inorganic materials 0.000 description 1
- 238000004519 manufacturing process Methods 0.000 description 1
- 230000004060 metabolic process Effects 0.000 description 1
- 239000011707 mineral Substances 0.000 description 1
- 235000016709 nutrition Nutrition 0.000 description 1
- 230000035764 nutrition Effects 0.000 description 1
- 230000000050 nutritive effect Effects 0.000 description 1
- 238000009400 out breeding Methods 0.000 description 1
- 235000021017 pears Nutrition 0.000 description 1
- 239000000575 pesticide Substances 0.000 description 1
- 230000001737 promoting effect Effects 0.000 description 1
- 241000894007 species Species 0.000 description 1
- 230000006641 stabilisation Effects 0.000 description 1
- 238000011105 stabilization Methods 0.000 description 1
- 235000018553 tannin Nutrition 0.000 description 1
- 229920001864 tannin Polymers 0.000 description 1
- 239000001648 tannin Substances 0.000 description 1
- 238000012090 tissue culture technique Methods 0.000 description 1
- 239000011701 zinc Substances 0.000 description 1
- 229910052725 zinc Inorganic materials 0.000 description 1
Classifications
-
- Y02P60/216—
Landscapes
- Cultivation Of Plants (AREA)
Abstract
The invention discloses a cultivation method of seedless roxburgh roses, which takes a stem lip of a single plant of excellent seedless roxburgh roses as an explant, adopts the method of tissue culture to cultivate seedlings, and then moves the seedlings to a nursery for further growth and reproduction. The method of the tissue culture adopted by the invention overcomes the defects that conventional breeding of roxburgh rose seedlings has a long period and the cuttage has a low propagation coefficient. Through the tissue culture, a great amount of tube seedlings with stable inheritable character can be obtained, excellent parent property can be maintained; in addition, the material is easy to get, the aberration rate is low, the proliferation coefficient is high, and the rooting percentage is up to 90%, the reproduction rate is greatly increased; and moreover, the reproduction method has a stable property, the experiment has a good repeatability, the tube seedlings differentiate in an excellent manner, the investment is low, the period is short. As a result, both growth and market popularization of seedless roxburgh roses are promoted.
Description
Technical field
The invention belongs to field of plant growing technology, particularly relate to a kind of cultivation method without the seed Rosa roxburghii.
Background technology
Anshun belongs to rose family machaka without seed Rosa roxburghii (Anshun gold Rosa roxburghii), is the sibling species of common Rosa roxburghii, and high approximately 2 meters of plant has the property of climbing up by holding on to, multi-branched, and the hat width of cloth reaches 4-6 meters, and very similar to the Guizhou rosa roxburghii on botanical character, affiliation is very approaching.Without seed Rosa roxburghii mean fruit weight 5-9g, maximum single fruit weight can reach 12g, pericarp yellowish-brown, close living spinule, and spinule very easily comes off when ripe, and it is gorgeous orange-yellow, plump tender and crisp that pulp is.Its stamen height abortion, flower pesticide is shrivelled, without pollen, 1-2 grain achene seed occurs once in a while.
Abundant without seed rosa roxburghii nutrition composition, the sugar content of fruit is higher, measure by analysis, be more than 6 times of common Rosa roxburghii, contain acid lower, several without tannin, be rich in vitamin C, the antioxidases such as SOD, carotene carotene content is higher, the several amino acids of the several mineral materials nutritive element such as calcic, zinc and needed by human body.The gold Rosa roxburghii not only has the effect of promoting the production of body fluid, refresh oneself, whetting the appetite, and also has the auxiliary enteron aisle digestion of nature, improves the function of human body Vc content, for the old and the weak patient, appetizing improves a poor appetite especially, and defaecation promotes the tonic of new one-tenth metabolism, contain abundant vitamin C, be called as " king of Vc ".Its tonic, the effect of building up one's health by taking tonic are obvious, and be so we are called ' fruit of prolonging life ', qualified to the effect that human body is promoted longevity.And sour-sweet suitable, the few astringent taste of fruit, mouthfeel obviously is better than common Rosa roxburghii, and market prospects are wide.
Easily produce adventive root without the seed Rosa roxburghii because of its branch, form indefinite bud, generally adopt the method for cuttage to carry out breeding and seedling at present.In recent years, tissue culture technique all is being widely used aspect fruit tree fast seedling growing and the virus-free seedling of cultivation, but it is low to have reproduction coefficient for the group training aspect without the seed Rosa roxburghii, and rooting rate is low, the problems such as leaf regeneration difficulty.
Summary of the invention
For the problems referred to above, the invention provides with a kind of cultivation method without the seed Rosa roxburghii, adopt the Rosa roxburghii stem apex to cultivate as explant, raising reproduction coefficient and the rooting rate of efficiency solve the problems such as leaf regeneration difficulty.
For addressing the above problem, the technical scheme that the present invention takes is:
A kind of cultivation method without the seed Rosa roxburghii is characterized in that,, breeds in the steps below and cultivates as explant with good stem apex without seed Rosa roxburghii individual plant:
1) will gather the stem apex that comes rinses well with clear water, place the alcohol of 75-78% to soak 20-30s, soak 6-10min with the 5-7% liquor natrii hypochloritis again, then use aseptic water washing 3-5 time, then on superclean bench, stem apex is pruned, deduct the wound end that soaked through liquid, induce cultivation with carrying out test-tube plantlet on the inducing culture of pruning after good stem apex inserts sterilization, cultivation temperature is 22-25 ℃, humidity 65%--85%, intensity of illumination 1000-2000lx, light application time 8-10h/d, and incubation time is 28-30d;
2) carry out culture of rootage on the root media after the test-tube plantlet seedling is placed on sterilization, in cultivation temperature is 22-28 ℃, the environment of humidity 60%--80%, intensity of illumination 2000-3000lx, light application time 10-15h/d, cultivate 28-34d, make it grow up to complete test-tube plantlet;
3) test-tube plantlet is heeled in the little alms bowl that crushed crude pearlite is housed, after watering it is moved in the little shed of plastics and cultivated 10-15 days, cultivation temperature is 25-28 ℃, humidity 65%--85%, intensity of illumination 2000-3500lx, light application time 10-15h/d;
4) group in the little alms bowl of crushed crude pearlite is trained transplantation of seedlings to the humus soil case bed that is covered with through sterilization, water permeable, place and cultivate 8-12d in the little shed of plastics, cultivation temperature is 25-28 ℃, humidity 60%--70%, intensity of illumination 2500-4000lx, light application time 12-18h/d;
5) remove little shed, will organize the training seedling and in cultivation temperature is 25-30 ℃, the environment of humidity 55%--70%, intensity of illumination 3000-4200lx, light application time 10-15h/d, cultivate 30-32d, then be transplanted in the open country nursery.
Further, inducing culture in the described step 1) is: MS is as minimal medium, the sucrose of GA, the 15～30g/L of IBA, the 0.8～3.5mg/L of 6-BA, the 0.1～0.35mg/L of additional 0.6-1.0mg/L, the agar of 5～12g/L, Medium's PH Value is 5.5-6.5.
The stem apex length of further, pruning in the described step 1) is: 0.5-1cm.
Further, the root media described step 2) is: MS adds the sucrose of IBA, the 40～80g/L of 0.5～1.2mg/L, the agar of 5～12g/L as minimal medium, and Medium's PH Value is 6.0-6.5.
Further, described step 2) the long 1.5-3cm of test-tube plantlet in after the culture of rootage, the root of test-tube plantlet is the 1-14 bar, root is long to be 1.5-3cm.
To organize the training seedling, to be transplanted to draining good, plants on deep, the fertile loam of soil layer; Then carry out fertilizing management and rural area management work, cultivated plant is carried out in good time pruning and deinsectization work, guarantee the good growing way without the seed Rosa roxburghii.Gathered when beginning to transfer yellowish red color to by green at fruit complete ripeness, pericarp.Put into sieve after gathering or bamboo basket sieves deburring, to improve commodity.
Beneficial effect of the present invention is: the present invention uses the means that tissue is cultivated, overcome the pears seedling conventional breeding cycle long, the characteristics such as the cottage propagation coefficient is low, cultivate the test-tube plantlet that can obtain a large amount of stabilization characteristics of genetics by tissue, keep good parent's characteristic, and draw materials easily, aberration rate is low, growth coefficient is high, and rooting rate reaches 90%, greatly improved reproduction speed, and the propagation method stable performance, test repeatability is good, the test-tube plantlet well differentiated, less investment, cycle is short, has promoted greatly the plantation without the seed Rosa roxburghii, has promoted the marketing without the seed Rosa roxburghii.
Embodiment
The invention will be further described below in conjunction with specific embodiment, is used for explaining the present invention in illustrative examples and the explanation of this invention, but not as a limitation of the invention.
Embodiment 1
Preparation medium and sterilization:
A inducing culture: MS is as minimal medium, the sucrose of GA, the 25g/L of IBA, the 2mg/L of 6-BA, the 0.25mg/L of additional 0.8mg/L, the agar of 8g/L, and Medium's PH Value is 6.0.
B root media: MS adds the sucrose of IBA, the 60g/L of 0.7mg/L, the agar of 10g/L as minimal medium, and Medium's PH Value is 6.2.
1) will gather the stem apex that comes rinses well with clear water, place 76% alcohol to soak 25s, soak 8min with 6% liquor natrii hypochloritis again, then use aseptic water washing 4 times, then on superclean bench, stem apex is pruned, deduct the wound end that soaked through liquid, stem apex length is 0.8cm, induce cultivation with carrying out test-tube plantlet on the inducing culture of pruning after good stem apex inserts sterilization, the cultivation temperature of inducing culture is 23 ℃, humidity 70%, intensity of illumination 1500lx, light application time 9h/d, and incubation time is 29d;
2) carry out culture of rootage on the root media after the test-tube plantlet seedling is placed on sterilization, in being 26 ℃, the environment of humidity 70%, intensity of illumination 2500lx, light application time 13h/d, cultivation temperature cultivates 30d, make it grow up to complete test-tube plantlet, the long 2-2.5cm of test-tube plantlet after the culture of rootage, the root of test-tube plantlet is the 5-10 bar, and root is long to be 1.5-3cm.；
3) test-tube plantlet is heeled in the little alms bowl that crushed crude pearlite is housed, after watering it is moved in the little shed of plastics and cultivated 13 days, cultivation temperature is 27 ℃, humidity 75%, intensity of illumination 3000lx, light application time 13h/d;
4) the group in the little alms bowl of crushed crude pearlite training transplantation of seedlings to the humus soil case bed that is covered with through sterilization, water permeablely, place and cultivate 10d in the little shed of plastics, cultivation temperature is 26 ℃, humidity 65%, intensity of illumination 3200lx, light application time 16h/d;
5) remove little shed, group training seedling is cultivated 30d in cultivation temperature is 26 ℃, the environment of humidity 65%, intensity of illumination 3400lx, light application time 13h/d, then be transplanted in the open country nursery.
To organize the training seedling good in draining, plant on deep, the fertile loam of soil layer.Dig planting pit by 4 * 3 meters distances between rows and hills, the planting pit length and width is 80 centimetres, dark 50 centimetres.When digging pit, table soil separates with cubsoil, will show earthen backfill during backfill and enter the hole, and mix thoroughly with 30 kilograms of farmyard manures, 0.5 kilogram of Chemical Mixed Fertilizer, and then cover the fine earth of 10 cm thicks, continues backfill and bulldozes 20 centimetres above ground level of mound.In field planting mid-February of late November to next year, every mu of plantation 80 strains.
During field planting, dig 12 centimetres shallow hole at the center, field planting hole that backfill is good, nursery stock is placed hole central authorities, the arrangement root system evenly distributes, implant fine earth, again nursery stock is slightly upwards carried, its root system is trailed, jam on tight soil while banketing, plant completely, irrigate normal root water, strengthen management, to preserve work.
Used base manure in 1 year 1 time, impose 3 times.Base manure is selected the fertilizer that becomes thoroughly decomposed, suitably with addition of a certain amount of compound, every mu of orchard application of organic fertilizers 1200kg, Chemical Mixed Fertilizer 45kg of getting.Before taking out the tip February, impose once, use 20kg for every mu, spray 0.4% potassium dihydrogen phosphate 1 time.
In without seed Rosa roxburghii process of growth, note pruning and the control of insect pest tree-like.Gathered when beginning to transfer yellowish red color to by green at fruit complete ripeness, pericarp.Put into sieve after gathering or bamboo basket sieves deburring, to improve commodity.
Embodiment 2
Preparation medium and sterilization:
A inducing culture: MS is as minimal medium, the sucrose of GA, the 15g/L of IBA, the 0.8mg/L of 6-BA, the 0.1mg/L of additional 0.6mg/L, the agar of 5g/L, and Medium's PH Value is 5.5.
B root media: MS adds the sucrose of IBA, the 40g/L of 0.5mg/L, the agar of 5g/L as minimal medium, and Medium's PH Value is 6.0.
1) will gather the stem apex that comes rinses well with clear water, place 75% alcohol to soak 30s, soak 6min with 5% liquor natrii hypochloritis again, then use aseptic water washing 3 times, then on superclean bench, stem apex is pruned, deduct the wound end that soaked through liquid, stem apex length is 0.5cm, induce cultivation with carrying out test-tube plantlet on the inducing culture of pruning after good stem apex inserts sterilization, the cultivation temperature of inducing culture is 22 ℃, humidity 65%, intensity of illumination 1000lx, light application time 8h/d, and incubation time is 28d;
2) carry out culture of rootage on the root media after the test-tube plantlet seedling is placed on sterilization, in being 22 ℃, the environment of humidity 60%, intensity of illumination 2000lx, light application time 10h/d, cultivation temperature cultivates 28d, make it grow up to complete test-tube plantlet, the long 1.5-2.0cm of test-tube plantlet after the culture of rootage, the root of test-tube plantlet is the 1-8 bar, and root is long to be 1.5-3cm.；
3) test-tube plantlet is heeled in the little alms bowl that crushed crude pearlite is housed, after watering it is moved in the little shed of plastics and cultivated 10 days, cultivation temperature is 25 ℃, humidity 65%, intensity of illumination 2000lx, light application time 10h/d;
4) the group in the little alms bowl of crushed crude pearlite training transplantation of seedlings to the humus soil case bed that is covered with through sterilization, water permeablely, place and cultivate 8d in the little shed of plastics, cultivation temperature is 25 ℃, humidity 60%, intensity of illumination 2500lx, light application time 12h/d;
5) remove little shed, group training seedling is cultivated 31d in cultivation temperature is 25 ℃, the environment of humidity 55%, intensity of illumination 3000lx, light application time 10h/d, then be transplanted in the open country nursery.
To organize the training seedling good in draining, plant on deep, the fertile loam of soil layer.Dig planting pit by 4 * 3 meters distances between rows and hills, the planting pit length and width is 80 centimetres, dark 50 centimetres.When digging pit, table soil separates with cubsoil, will show earthen backfill during backfill and enter the hole, and mix thoroughly with 25 kilograms of farmyard manures, 0.5 kilogram of Chemical Mixed Fertilizer, and then cover the fine earth of 10 cm thicks, continues backfill and bulldozes 20 centimetres above ground level of mound.In field planting mid-February of late November to next year, every mu of plantation 80 strains.
During field planting, dig 12 centimetres shallow hole at the center, field planting hole that backfill is good, nursery stock is placed hole central authorities, the arrangement root system evenly distributes, implant fine earth, again nursery stock is slightly upwards carried, its root system is trailed, jam on tight soil while banketing, plant completely, irrigate normal root water, strengthen management, to preserve work.
Used base manure in 1 year 1 time, impose 3 times.Base manure is selected the fertilizer that becomes thoroughly decomposed, suitably with addition of a certain amount of compound, every mu of orchard application of organic fertilizers 1500kg, Chemical Mixed Fertilizer 50kg of getting.Before taking out the tip February, impose once, use 30kg for every mu, spray 0.5% potassium dihydrogen phosphate 1 time.
In without seed Rosa roxburghii process of growth, note pruning and the control of insect pest tree-like.Gathered when beginning to transfer yellowish red color to by green at fruit complete ripeness, pericarp.Put into sieve after gathering or bamboo basket sieves deburring, to improve commodity.
Embodiment 3
Preparation medium and sterilization:
A inducing culture: MS is as minimal medium, the sucrose of GA, the 30g/L of IBA, the 3.5mg/L of 6-BA, the 0.35mg/L of additional 1.0mg/L, the agar of 12g/L, and Medium's PH Value is 6.5.
B root media: MS adds the sucrose of IBA, the 80g/L of 1.2mg/L, the agar of 12g/L as minimal medium, and Medium's PH Value is 6.5.
1) will gather the stem apex that comes rinses well with clear water, place 78% alcohol to soak 20s, soak 10min with 7% liquor natrii hypochloritis again, then use aseptic water washing 5 times, then on superclean bench, stem apex is pruned, deduct the wound end that soaked through liquid, stem apex length is 1cm, induce cultivation with carrying out test-tube plantlet on the inducing culture of pruning after good stem apex inserts sterilization, the cultivation temperature of inducing culture is 25 ℃, humidity 85%, intensity of illumination 2000lx, light application time 10h/d, and incubation time is 30d;
2) carry out culture of rootage on the root media after the test-tube plantlet seedling is placed on sterilization, in being 28 ℃, the environment of humidity 80%, intensity of illumination 3000lx, light application time 15h/d, cultivation temperature cultivates 34d, make it grow up to complete test-tube plantlet, the long 2-3cm of test-tube plantlet after the culture of rootage, the root of test-tube plantlet is the 5-14 bar, and root is long to be 1.5-3cm.；
3) test-tube plantlet is heeled in the little alms bowl that crushed crude pearlite is housed, after watering it is moved in the little shed of plastics and cultivated 15 days, cultivation temperature is 28 ℃, humidity 85%, intensity of illumination 3500lx, light application time 15h/d;
4) the group in the little alms bowl of crushed crude pearlite training transplantation of seedlings to the humus soil case bed that is covered with through sterilization, water permeablely, place and cultivate 12d in the little shed of plastics, cultivation temperature is 28 ℃, humidity 70%, intensity of illumination 4000lx, light application time 18h/d;
5) remove little shed, group training seedling is cultivated 32d in cultivation temperature is 30 ℃, the environment of humidity 70%, intensity of illumination 4200lx, light application time 15h/d, then be transplanted in the open country nursery.
To organize the training seedling good in draining, plant on deep, the fertile loam of soil layer.Dig planting pit by 3 * 2.5 meters distances between rows and hills, the planting pit length and width is 80 centimetres, dark 50 centimetres.When digging pit, table soil separates with cubsoil, will show earthen backfill during backfill and enter the hole, and mix thoroughly with 20 kilograms of farmyard manures, 0.5 kilogram of Chemical Mixed Fertilizer, and then cover the fine earth of 10 cm thicks, continues backfill and bulldozes 20 centimetres above ground level of mound.In field planting mid-February of late November to next year, every mu of plantation 90 strains.
During field planting, dig 12 centimetres shallow hole at the center, field planting hole that backfill is good, nursery stock is placed hole central authorities, the arrangement root system evenly distributes, implant fine earth, again nursery stock is slightly upwards carried, its root system is trailed, jam on tight soil while banketing, plant completely, irrigate normal root water, strengthen management, to preserve work.
Used base manure in 1 year 1 time, impose 3 times.Base manure is selected the fertilizer that becomes thoroughly decomposed, suitably with addition of a certain amount of compound, every mu of orchard application of organic fertilizers 1000kg, Chemical Mixed Fertilizer 40kg of getting.Before taking out the tip February, impose once, use 15kg for every mu, spray 0.3% potassium dihydrogen phosphate 2 times.
In without seed Rosa roxburghii process of growth, note pruning and the control of insect pest tree-like.Gathered when beginning to transfer yellowish red color to by green at fruit complete ripeness, pericarp.Put into sieve after gathering or bamboo basket sieves deburring, to improve commodity.
Technical scheme of the present invention is not limited to the restriction of above-mentioned specific embodiment, and the technology distortion that every technical scheme according to the present invention is made all falls within protection scope of the present invention.
Claims (5)
1. the cultivation method without the seed Rosa roxburghii is characterized in that,, breeds in the steps below and cultivates as explant with good stem apex without seed Rosa roxburghii individual plant:
1) will gather the stem apex that comes rinses well with clear water, place the alcohol of 75-78% to soak 20-30s, soak 6-10min with the 5-7% liquor natrii hypochloritis again, then use aseptic water washing 3-5 time, then on superclean bench, stem apex is pruned, deduct the wound end that soaked through liquid, induce cultivation with carrying out test-tube plantlet on the inducing culture of pruning after good stem apex inserts sterilization, cultivation temperature is 22-25 ℃, humidity 65%--85%, intensity of illumination 1000-2000lx, light application time 8-10h/d, and incubation time is 28-30d;
2) carry out culture of rootage on the root media after the test-tube plantlet seedling is placed on sterilization, in cultivation temperature is 22-28 ℃, the environment of humidity 60%--80%, intensity of illumination 2000-3000lx, light application time 10-15h/d, cultivate 28-34d, make it grow up to complete test-tube plantlet;
3) test-tube plantlet is heeled in the little alms bowl that crushed crude pearlite is housed, after watering it is moved in the little shed of plastics and cultivated 10-15 days, cultivation temperature is 25-28 ℃, humidity 65%--85%, intensity of illumination 2000-3500lx, light application time 10-15h/d;
4) group in the little alms bowl of crushed crude pearlite is trained transplantation of seedlings to the humus soil case bed that is covered with through sterilization, water permeable, place and cultivate 8-12d in the little shed of plastics, cultivation temperature is 25-28 ℃, humidity 60%--70%, intensity of illumination 2500-4000lx, light application time 12-18h/d;
5) remove little shed, will organize the training seedling and in cultivation temperature is 25-30 ℃, the environment of humidity 55%--70%, intensity of illumination 3000-4200lx, light application time 10-15h/d, cultivate 30-32d, then be transplanted in the open country nursery.
2. the cultivation method without the seed Rosa roxburghii according to claim 1, it is characterized in that: the inducing culture in the described step 1) is: MS is as minimal medium, the sucrose of GA, the 15～30g/L of IBA, the 0.8～3.5mg/L of 6-BA, the 0.1～0.35mg/L of additional 0.6-1.0mg/L, the agar of 5～12g/L, Medium's PH Value is 5.5-6.5.
3. the cultivation method without the seed Rosa roxburghii according to claim 1, it is characterized in that: the stem apex length of pruning in the described step 1) is: 0.5-1cm.
4. the cultivation method without the seed Rosa roxburghii according to claim 1, it is characterized in that: the root media described step 2) is: MS is as minimal medium, the sucrose of IBA, the 40～80g/L of additional 0.5～1.2mg/L, the agar of 5～12g/L, Medium's PH Value is 6.0-6.5.
5. the cultivation method without the seed Rosa roxburghii according to claim 1 is characterized in that: the long 1.5-3cm of test-tube plantlet described step 2) after the culture of rootage, and the root of test-tube plantlet is the 1-14 bar, root is long to be 1.5-3cm.
Priority Applications (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100103588A CN103070070A (en) | 2013-01-10 | 2013-01-10 | Cultivation method of seedless roxburgh roses |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| CN2013100103588A CN103070070A (en) | 2013-01-10 | 2013-01-10 | Cultivation method of seedless roxburgh roses |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| CN103070070A true CN103070070A (en) | 2013-05-01 |
Family
ID=48146938
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| CN2013100103588A Pending CN103070070A (en) | 2013-01-10 | 2013-01-10 | Cultivation method of seedless roxburgh roses |
Country Status (1)
| Country | Link |
|---|---|
| CN (1) | CN103070070A (en) |
Cited By (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103229692A (en) * | 2013-05-05 | 2013-08-07 | 安顺市西秀区胜学原生态种养植生态园 | Cultivation method for seedless roxburgh roses |
| CN103416276A (en) * | 2013-08-29 | 2013-12-04 | 贵州兴黔伟业生态科技开发有限公司 | Roxburgh rose seedling raising method |
| CN104541869A (en) * | 2014-12-29 | 2015-04-29 | 普定县裕源鼎金刺梨种植专业合作社 | Cuttage breeding method of Rosa sterilis var. leioclada |
| CN104770177A (en) * | 2015-04-10 | 2015-07-15 | 贵州师范大学 | Rosa roxburghii ex-vivo twig culturing method and application thereof in stony desertification control |
| CN106718517A (en) * | 2016-11-24 | 2017-05-31 | 遵义县顺龙发种植场 | A kind of implantation methods of Rosa roxburghii |
-
2013
- 2013-01-10 CN CN2013100103588A patent/CN103070070A/en active Pending
Cited By (6)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103229692A (en) * | 2013-05-05 | 2013-08-07 | 安顺市西秀区胜学原生态种养植生态园 | Cultivation method for seedless roxburgh roses |
| CN103416276A (en) * | 2013-08-29 | 2013-12-04 | 贵州兴黔伟业生态科技开发有限公司 | Roxburgh rose seedling raising method |
| CN103416276B (en) * | 2013-08-29 | 2014-10-22 | 贵州兴黔伟业生态科技开发有限公司 | Roxburgh rose seedling raising method |
| CN104541869A (en) * | 2014-12-29 | 2015-04-29 | 普定县裕源鼎金刺梨种植专业合作社 | Cuttage breeding method of Rosa sterilis var. leioclada |
| CN104770177A (en) * | 2015-04-10 | 2015-07-15 | 贵州师范大学 | Rosa roxburghii ex-vivo twig culturing method and application thereof in stony desertification control |
| CN106718517A (en) * | 2016-11-24 | 2017-05-31 | 遵义县顺龙发种植场 | A kind of implantation methods of Rosa roxburghii |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| CN104081980B (en) | A kind of Pasania cuspidata container seedling culture method | |
| CN105557427A (en) | Planting method of selenium-rich passiflora edulis | |
| CN109496734A (en) | Method and a kind of ferment rice using ferment rice cultivation | |
| CN102119611B (en) | Artificial domestication technology of panicled fameflower root | |
| CN106416900A (en) | Planting method for promoting production increase at earlier stage of sweet cherries | |
| CN107182789A (en) | The standardized cultivation method of bletilla | |
| CN105145104A (en) | Method for segmenting bletilla striata tubers to perform seedling propagation and raising | |
| CN110972864A (en) | Selenium-rich cultivation method for Bigheng white atractylodes rhizome | |
| CN115250812A (en) | A kind of blueberry seed germination method and propagation and seedling raising method | |
| CN109380069A (en) | A kind of implantation methods of tomato | |
| CN104429785A (en) | High-quality and efficient golden bell tree seedling growing method | |
| CN111837786A (en) | Cultivation method of multi-variety oil tea | |
| CN105104051A (en) | Cultivation method for high-yield Chinese scholartree | |
| CN103070070A (en) | Cultivation method of seedless roxburgh roses | |
| CN100364377C (en) | A method for cultivating herbaceous plant seedlings adapted to alpine habitats | |
| CN104303779B (en) | A kind of cultivation method promoting food sweet potato block root quality | |
| CN104770177B (en) | A kind of Rosa roxburghii isolated shoot method for culturing seedlings and the application in Rocky Desertification Control | |
| CN107509570A (en) | A kind of ecology planting method suitable for northern area rice quantitative selenium-rich | |
| CN109997594A (en) | A kind of late-maturing cultural method of plateau mango | |
| CN108990734A (en) | A kind of selenium-rich Chinese yam cultivation method | |
| CN106069671A (en) | Be suitable to two sections of substrate cultivating methods of fruit tree hybrid Seedling of greenhouse second crop soil | |
| CN110915577B (en) | Angelica keiskei seedling transplanting method | |
| CN105875412B (en) | The implantation methods of organic selenium-rich sugariness gynostemma pentaphylla | |
| CN111903443A (en) | Cultivation method of dandelion | |
| CN111034518A (en) | Blood orange planting method |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| C06 | Publication | ||
| PB01 | Publication | ||
| C02 | Deemed withdrawal of patent application after publication (patent law 2001) | ||
| WD01 | Invention patent application deemed withdrawn after publication |
Application publication date: 20130501 |