CN103059623B - PET (polyethylene terephthalate) impermeable membranization agent, impermeable membranization PET blood collection tube, and corresponding preparation methods - Google Patents

Abstract

The invention discloses a polyester anti-seepage membrane agent, an anti-seepage membrane polyester blood collection tube and a corresponding preparation method. Polyester anti-seepage membrane agent includes the following ingredients: hydrophilic silicone, polyethylene glycol 1000, acrylamide, sodium hydroxymethylcellulose, polyvinylpyrrolidone, silica gel H60, hydroxyethyl starch, trimethylolpropane, Alcohol ether ester diethanolamine salt antistatic liquid, JNSO-2000 long-acting antibacterial agent, absolute ethanol. The invention has the advantages of solving the problem of water penetration of the polyester (PET) blood collection tube, and the anti-seepage membrane polyester (PET) blood collection tube has the remarkable feature of waterproof penetration. The invention also discloses a preparation method of the polyester anti-seepage membrane agent, an anti-seepage membrane polyester blood collection tube using the polyester anti-seepage membrane agent as an inner wall coating treatment agent and a preparation method thereof.

Description

Polyester anti-seepage membrane agent, anti-seepage membrane polyester blood collection tube and corresponding preparation method

technical field

The invention relates to an anti-seepage membrane inner wall coating treatment agent and an anti-seepage treatment agent for polyester PET (scientific name: polyethylene terephthalate, English: Polyethylene terephthalate) material containing liquid anticoagulant in medical clinical examination The invention relates to a method for preparing a membrane-formed polyester PET blood collection tube, in particular to a polyester anti-seepage film-forming agent and a preparation method, and an anti-seepage film-form polyester film using the polyester anti-seepage film-forming agent as an inner wall coating treatment agent. Blood collection tube and its preparation method.

Background technique

At present, there are two types of blood collection tubes used for blood sample collection in clinical medical examinations, one is made of glass, and the other is made of polyester (PET). Glass blood collection tubes are fragile, especially not suitable for the collection of precious and rare samples or samples that need to be frozen and transported, and they are not easy to dispose of after use (usually need to be crushed and landfilled). The polyester (PET) material blood collection tube is transparent, non-toxic and not easy to break. Anti-permeability treatment is a worldwide problem. For blood collection tubes containing anticoagulants, the ratio of anticoagulant solution to anticoagulant blood is required to be fixed (such as blood coagulation blood collection tubes: 109 mmol/L sodium citrate and anticoagulant Blood coagulation ratio 1:9; erythrocyte sedimentation blood collection tube: 109mmol/L sodium citrate and anticoagulant blood ratio 1:4; blood cell analysis blood collection tube: 200g/L ethylenediaminetetraacetic acid dipotassium or tripotassium (EDTA-2K or 3K ) 10μl anticoagulated 1ml blood, etc.). Once the water in the hemagglutination and erythrocyte sedimentation tube seeps out, the ratio of anticoagulant to blood sample will change, seriously affecting the measurement results. However, the anticoagulant edetate dipotassium or tripotassium used in blood cell analysis blood collection tubes has a large concentration (200g/L) and a small amount (generally 20μl anticoagulant anticoagulant 2ml of blood), once the water in the blood collection tube seeps slightly The thick and irregular crystals unique to EDTA potassium salt will appear immediately, and it is very difficult to dissolve in the blood sample during blood collection and cannot achieve anticoagulant effect. Generally, it is required to mix upside down for more than 8 times, or even more than ten times, which is very easy to cause hemolysis. and platelet fragmentation. At present, there are a small number of manufacturers in China who put a small polypropylene (PP) tube inside the PET tube body to prevent water penetration; there are also manufacturers who pack a layer of tin foil for every 10 or 20 PET blood collection tubes for waterproofing. These improvement measures are not effective. Good, but also a waste of resources. Researching and inventing anti-seepage membrane polyester PET blood collection tubes is an urgent problem and value that needs to be solved in clinical laboratory medicine.

Contents of the invention

In order to solve the problem of water penetration in the body of polyester (PET) blood collection tubes, it is necessary for the present invention to provide a polyester anti-seepage membrane agent and a preparation method, and a method using the polyester anti-seepage membrane agent as an inner wall coating treatment agent. The anti-seepage membrane polyester blood collection tube and its preparation method.

The present invention is achieved in this way, a polyester anti-seepage membrane agent, which includes the following components: hydrophilic silicone, polyethylene glycol 1000, acrylamide, sodium hydroxymethyl cellulose, polyvinylpyrrolidone, silica gel H60, Hydroxyethyl starch, trimethylolpropane, alcohol ether ester diethanolamine salt antistatic liquid, JNSO-2000 long-acting antibacterial agent, absolute ethanol.

Preferably, hydrophilic silicone, polyethylene glycol 1000, acrylamide, sodium hydroxymethylcellulose, polyvinylpyrrolidone, silica gel H60, hydroxyethyl starch, trimethylolpropane, glycol ether ester diethanolamine salt The weight ratio between the electrostatic liquid, JNSO-2000 long-acting antibacterial agent and absolute ethanol is 3.0:5.0:2.0:1.5:4.0-6.0:1.2:1.0:1.5-3.5:2.0-2.6:0.2:76.8.

The present invention also provides a preparation method of a polyester anti-seepage membrane agent, which comprises the following components: hydrophilic silicone, polyethylene glycol 1000, acrylamide, sodium hydroxymethyl cellulose, Polyvinylpyrrolidone, silica gel H60, hydroxyethyl starch, trimethylolpropane, alcohol ether ester diethanolamine salt antistatic liquid, JNSO-2000 long-acting antibacterial agent, absolute ethanol. The preparation method comprises the following steps:

Raw materials: hydrophilic silicone, polyethylene glycol 1000, acrylamide, sodium hydroxymethylcellulose, polyvinylpyrrolidone, silica gel H60, hydroxyethyl starch, trimethylolpropane, alcohol ether ester diethanolamine salt resistance Electrostatic liquid, JNSO-2000 long-acting antibacterial agent, absolute ethanol;

The above raw materials are homogenized and subjected to ultrasonic ultrafiltration to obtain a transparent and stable microemulsion colloid, which is the polyester anti-seepage membrane agent.

Preferably, the above-mentioned raw materials are homogenized by a homogenizer, and ultra-filtered by 40KHZ high-power ultrasonic waves to obtain a transparent and stable microemulsion colloid, which is the polyester anti-seepage membrane agent.

Preferably, raw materials are prepared: parts by weight: 3.0 of hydrophilic silicone, 5.0 of polyethylene glycol 1000, 2.0 of acrylamide, 1.5 of sodium hydroxymethyl cellulose, 4.0-6.0 of polyvinylpyrrolidone, 1.2 of Silica gel H60, 1.0 hydroxyethyl starch, 1.5-3.5 trimethylolpropane, 2.0-2.6 alcohol ether ester diethanolamine salt antistatic liquid, 0.2 JNSO-2000 long-acting antibacterial agent, 76.8 absolute ethanol .

The invention also provides an anti-seepage membrane polyester blood collection tube, the inner wall of which is coated with polyester anti-seepage membrane agent. The polyester anti-seepage membrane agent includes the following components: hydrophilic silicone, polyethylene glycol 1000, acrylamide, sodium hydroxymethylcellulose, polyvinylpyrrolidone, silica gel H60, hydroxyethyl starch, trimethylolpropane , alcohol ether ester diethanolamine salt antistatic liquid, JNSO-2000 long-acting antibacterial agent, absolute ethanol.

Preferably, in the coating, hydrophilic silicone, polyethylene glycol 1000, acrylamide, sodium hydroxymethylcellulose, polyvinylpyrrolidone, silica gel H60, hydroxyethyl starch, trimethylolpropane, glycol ether acid The weight ratio between ester diethanolamine salt antistatic liquid, JNSO-2000 long-acting antibacterial agent and absolute ethanol is 3.0:5.0:2.0:1.5:4.0-6.0:1.2:1.0:1.5-3.5:2.0-2.6:0.2 : 76.8.

The present invention also provides a method for preparing an anti-seepage membraneized polyester blood collection tube, which includes a membraneization process, characterized in that: the membraneization process is:

Put the tube body of the polyester blood collection tube into a covered copper wire basket or stainless steel wire basket, with the mouth of the tube facing up, fasten the lid of the basket, and intrude sideways into the container of the polyester anti-seepage membrane agent, take it out for 30 minutes, put Pour out the polyester anti-seepage film agent with the mouth of the tube facing down. After completion, put the mouth of the tube upside down in the pool, and then use the deionized high-pressure atomized water nozzle to wash off the polyester anti-seepage film on the outer wall of the polyester blood collection tube body. The filming agent is then placed in a 40°C water-proof incubator for 2 hours, and the filming process is completed, and it is continued to be put into the production of the next process of the blood collection tube assembly line. Wherein, the polyester anti-seepage membrane agent includes the following components: hydrophilic silicone, polyethylene glycol 1000, acrylamide, sodium hydroxymethylcellulose, polyvinylpyrrolidone, silica gel H60, hydroxyethyl starch, trimethylol propane, alcohol ether ester diethanolamine salt antistatic liquid, JNSO-2000 long-acting antibacterial agent, absolute ethanol.

Preferably, in the polyester anti-seepage membrane agent, hydrophilic silicone, polyethylene glycol 1000, acrylamide, sodium hydroxymethylcellulose, polyvinylpyrrolidone, silica gel H60, hydroxyethyl starch, trimethylol The weight ratio between propane, alcohol ether ester diethanolamine salt antistatic liquid, JNSO-2000 long-acting antibacterial agent, and absolute ethanol is 3.0:5.0:2.0:1.5:4.0-6.0:1.2:1.0:1.5-3.5: 2.0-2.6:0.2:76.8.

Compared with the prior art, the polyester anti-seepage film-forming agent of the present invention and its preparation method, the anti-seepage film-formed polyester blood collection tube using the polyester anti-seepage film-forming agent as an inner wall coating treatment agent and its preparation method, It solves the problem of water penetration of polyester (PET) blood collection tubes well, and the anti-seepage membrane polyester (PET) blood collection tubes has a remarkable feature of waterproof penetration.

Detailed ways

In order to make the object, technical solution and advantages of the present invention more clear, the present invention will be further described in detail below in conjunction with the examples. It should be understood that the specific embodiments described here are only used to explain the present invention, not to limit the present invention.

1. Preparation of polyester anti-seepage membrane agent

The polyester anti-seepage membrane agent is characterized in that it includes the following raw materials in parts by weight

Hydrophilic silicone (water-soluble silicone oil) 3.0 Polyethylene glycol 1000 (PEG1000) 5.0 Acrylamide 2.0 sodium hydroxymethyl cellulose 1.5 Polyvinylpyrrolidone (PVP) 4.0-6.0 Silicone H60 1.2 Hydroxyethyl starch 1.0 Trimethylolpropane (TMP) 1.5-3.5 Alcohol Ether Ester Diethanolamine Salt Antistatic Liquid 2.0-2.6 JNSO-2000 long-acting antibacterial agent 0.2 Absolute ethanol 76.8

The above raw materials are homogenized by a homogenizer, and ultra-filtered by 40KHZ high-power ultrasonic to obtain a transparent and stable microemulsion colloid, which is the polyester anti-seepage membrane agent.

2. Membrane method for inner wall of polyester PET blood collection tube with anti-seepage membrane

Put the polyester (PET) blood collection tube body into a covered copper wire basket or stainless steel wire basket, with the mouth of the tube facing up, fasten the lid of the basket, and intrude sideways into the container of polyester anti-seepage membrane agent for 30 minutes Take it out from left to right, pour out the polyester anti-seepage film agent with the mouth of the tube down, and buckle it upside down in the pool after completion, and then wash away the polyester (PET) blood collection tube with a deionized high-pressure atomized water nozzle The anti-seepage membrane agent for the outer wall of the body is put into a 40°C water-proof incubator for 2 hours, and the membrane process is completed, and it is continued to be put into the production of the next process of the blood collection tube assembly line.

Experimental effect verification:

Experiment 1:

Take 10 anti-seepage filmed polyester PET blood collection tubes, and another 10 non-filmed polyester (PET) blood collection tubes, add 20 μm 200 g/L dipotassium ethylenediaminetetraacetic acid (EDTA2K) to each tube and Pre-set the same vacuum degree of 2ml, put it into the warehouse at room temperature, and observe and compare after 6 months.

Results (see Table 1): The EDTA2K solution in the anti-seepage filmed polyester (PET) blood collection tube still exists in the form of droplets; the water in the EDTA2K solution in the non-filmed polyester (PET) blood collection tube loses significantly , most of EDTA2K has been crystallized. When the crystallized EDTA2K polyester (PET) blood collection tube collects blood samples and mixes them upside down for a limited number of times, EDTA2K is still difficult to dissolve in the blood, and cannot play the role of complexing calcium ions in the blood to prevent coagulation, resulting in the collection of blood The sample freezes.

Experimental results comparison table 1

Experiment 2

Take 10 anti-seepage membrane polyester (PET) blood collection tubes, and another 10 polyester (PET) blood collection tubes without membrane treatment, add 109mmol/L sodium citrate 200μm to each tube, and also preset the vacuum degree 2ml, put it in the warehouse at room temperature, observe and compare after 6 months.

Results (see Table 2): After testing, the amount of sodium citrate solution in the anti-seepage membrane polyester (PET) blood collection tubes is all within 200 μιη ± 10%, which is in line with the pharmaceutical industry standard of the People's Republic of China yy0314-2007 "Disposable The maximum allowable tolerance of liquid additives for the use of human venous blood sample collection containers is ±10% of the nominal volume. However, the amount of sodium citrate solution in polyester (PET) blood collection tubes without membrane treatment is reduced, and even more than 50%-70% of the water is lost.

Experimental results comparison table 2 (unit: μι)

The experimental results of Experiment 1 and Experiment 2 prove that the polyester anti-seepage membrane agent has solved the problem of water penetration of polyester (PET) blood collection tubes, and the anti-seepage membrane polyester (PET) blood collection tubes has a remarkable feature of waterproof and permeable.

The above descriptions are only preferred embodiments of the present invention, and are not intended to limit the present invention. Any modifications, equivalent replacements and improvements made within the spirit and principles of the present invention should be included in the protection of the present invention. within range.

Claims (8)

1. A polyester anti-seepage membrane agent, characterized in that it comprises the following components: hydrophilic silicone, polyethylene glycol 1000, acrylamide, sodium hydroxymethylcellulose, polyvinylpyrrolidone, silica gel H60, hydroxyl Ethyl starch, trimethylolpropane, alcohol ether ester diethanolamine salt antistatic liquid, JNSO2000 long-acting antibacterial agent, absolute ethanol, the hydrophilic silicone, polyethylene glycol 1000, acrylamide, methylol The weight ratio between sodium cellulose, polyvinylpyrrolidone, silica gel H60, hydroxyethyl starch, trimethylolpropane, glycol ether ester diethanolamine salt antistatic liquid, JNSO2000 long-acting antibacterial agent, and absolute ethanol is 3.0: 5.0: 2.0: 1.5: 4.0-6.0: 1.2: 1.0: 1.5-3.5: 2.0-2.6: 0.2: 76.8. the 2. a preparation method of polyester anti-seepage membrane agent as claimed in claim 1, is characterized in that: it may further comprise the steps: Raw materials: hydrophilic silicone, polyethylene glycol 1000, acrylamide, sodium hydroxymethylcellulose, polyvinylpyrrolidone, silica gel H60, hydroxyethyl starch, trimethylolpropane, alcohol ether ester diethanolamine salt resistance Electrostatic liquid, JNSO2000 long-acting antibacterial agent, absolute ethanol; The above raw materials are homogenized and subjected to ultrasonic ultrafiltration to obtain a transparent and stable microemulsion colloid, which is the polyester anti-seepage membrane agent. the 3. the preparation method of polyester anti-seepage membrane agent as claimed in claim 2 is characterized in that: above-mentioned raw material is homogenized through homogenizer, with 40KHz high-power ultrasonic ultrafiltration, obtains transparent, stable microemulsion colloid , which is polyester anti-seepage membrane agent. the 4. The preparation method of polyester anti-seepage membrane agent as claimed in claim 2, is characterized in that: prepare raw material: take weight part and be: the hydrophilic silicone of 3.0, the polyethylene glycol 1000 of 5.0, the propylene of 2.0 Amide, sodium hydroxymethylcellulose at 1.5, polyvinylpyrrolidone at 4.0-6.0, silica gel H60 at 1.2, hydroxyethyl starch at 1.0, trimethylolpropane at 1.5-3.5, alcohol ether ester di at 2.0-2.6 Ethanolamine salt antistatic liquid, 0.2 JNSO2000 long-acting antibacterial agent, 76.8 absolute ethanol. the 5. An anti-seepage membrane polyester blood collection tube, characterized in that: the inner wall of the tube is membrane-coated with the polyester anti-seepage membrane agent as claimed in claim 1. the 6. The anti-seepage membrane polyester blood collection tube as claimed in claim 5, characterized in that: in the coating, hydrophilic silicone, polyethylene glycol 1000, acrylamide, sodium hydroxymethyl cellulose, polyethylene The weight ratio between pyrrolidone, silica gel H60, hydroxyethyl starch, trimethylolpropane, alcohol ether ester diethanolamine salt antistatic liquid, JNSO2000 long-acting antibacterial agent, and absolute ethanol is 3.0:5.0:2.0:1.5: 4.0-6.0: 1.2: 1.0: 1.5-3.5: 2.0-2.6: 0.2: 76.8. the 7. A preparation method of the anti-seepage membraneized polyester blood collection tube as claimed in claim 5, comprising a membraneization process, characterized in that: the membraneization process is: Put the polyester blood collection tube body into a covered copper wire basket or stainless steel wire basket, with the mouth of the tube facing up, fasten the basket cover, and intrude sideways into the container of the polyester anti-seepage membrane agent as claimed in claim 1 , take it out after 30 minutes, pour out the polyester anti-seepage film agent with the mouth of the nozzle down, and buckle the nozzle upside down in the pool after completion, and then wash off the polyester blood collection tube with a deionized high-pressure atomized water nozzle The polyester anti-seepage filming agent on the outer wall of the body is put into a 40°C water-proof incubator for 2 hours, and the filming process is completed, and it continues to be put into the production of the next process of the blood collection tube assembly line. the 8. The preparation method of anti-seepage membrane polyester blood collection tube as claimed in claim 7, characterized in that: in the polyester anti-seepage membrane agent, hydrophilic silicone, polyethylene glycol 1000, acrylamide, hydroxyl The weight ratio between sodium methylcellulose, polyvinylpyrrolidone, silica gel H60, hydroxyethyl starch, trimethylolpropane, alcohol ether ester diethanolamine salt antistatic liquid, JNSO2000 long-acting antibacterial agent, and dehydrated alcohol is 3.0:5.0:2.0:1.5:4.0-6.0:1.2:1.0:1.5-3.5:2.0-2.6:0.2:76.8. the