CN103041442A - Sodium alginate-based bone binding agent and preparation method thereof - Google Patents
Sodium alginate-based bone binding agent and preparation method thereof Download PDFInfo
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- CN103041442A CN103041442A CN2013100254696A CN201310025469A CN103041442A CN 103041442 A CN103041442 A CN 103041442A CN 2013100254696 A CN2013100254696 A CN 2013100254696A CN 201310025469 A CN201310025469 A CN 201310025469A CN 103041442 A CN103041442 A CN 103041442A
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Abstract
一种基于海藻酸钠的骨骼粘合剂及其制备方法，涉及一种用于硬组织损伤修复的粘合剂。提供一种在前人基础上改良的具有制备容易、粘结强度高、固化性能优良、生物相容性好等优点的以海藻酸钠、羧甲基纤维素钠和硫酸软骨素为材料的一种基于海藻酸钠的骨骼粘合剂及其制备方法。所述基于海藻酸钠的骨骼粘合剂按质量百分比的组成为：海藻酸钠：1﹪～10﹪；羧甲基纤维素钠：1﹪～10﹪；硫酸软骨素：1﹪～5﹪；补钙剂：0﹪～50﹪；余量为水。将海藻酸钠、羧甲基纤维素钠、硫酸软骨素和补钙剂加到水中，混合均匀，即得到基于海藻酸钠的骨骼粘合剂，所得的基于海藻酸钠的骨骼粘合剂为胶状。A sodium alginate-based bone adhesive and a preparation method thereof relate to an adhesive used for repairing hard tissue damage. To provide an improved material based on sodium alginate, sodium carboxymethylcellulose and chondroitin sulfate, which has the advantages of easy preparation, high bonding strength, excellent curing performance, and good biocompatibility. A sodium alginate-based bone adhesive and a preparation method thereof. The composition of the bone adhesive based on sodium alginate is as follows: sodium alginate: 1% to 10%; sodium carboxymethylcellulose: 1% to 10%; chondroitin sulfate: 1% to 5% ; Calcium supplement: 0﹪～50﹪; the balance is water. Sodium alginate, sodium carboxymethyl cellulose, chondroitin sulfate and calcium supplement are added to water, and mixed evenly to obtain a bone adhesive based on sodium alginate. The obtained bone adhesive based on sodium alginate is gelatinous.
Description
Technical field
The present invention relates to a kind of binding agent for sclerous tissues's injury repairing, especially relate to a kind of skeletal agglutinant based on sodium alginate take sodium alginate, sodium carboxymethyl cellulose and chondroitin sulfate as material and preparation method thereof.
Background technology
Fracture is common in the high-energy damage that traffic accident, earthquake, falling from high altitude etc. cause.The key for the treatment of fracture is fixed on and plays mastery reaction and decisive action in the treatment fixing.The effectively fixing of medium and small broken bone of fracturing is a difficult problem that needs to be resolved hurrily always.The fixing bad fracture that just can not guarantee is according to normal rule healing, and the generation malunion, delayed union or unconnected situation stay sequela.Therefore, being fixed in the orthopaedics therapy always is a very important problem, extremely comes into one's own.If use traditional fixed apparatus such as screw, Kirschner wire etc. that broken bone is fixed, the broken bone of Chang Yinwei is too little and can't implement, fixing by force may be so that broken bone again damages and splits and cause fracture reduction bad, the limbs that finally cause fracturing stay various sequela.
Adopting the orthopaedics binding agent that broken bone is carried out bonding resetting is a kind of trend for the treatment of.The orthopaedics binding agent is because of its special environment for use and application target, to binding agent require highly, require binding agent to take into account preferably clinical serviceability, higher building reason mechanical strength and outstanding biocompatibility.Although existing orthopaedics binding agent has the advantages such as easy-formation and adhesive property are good, but because most of traditional orthopaedics binding agent exists: the materials chemistry composition is fully different from the human bone composition, biocompatibility is general or relatively poor, degraded is slow or can not be degraded by human body, and the monomer that may discharge in the polymerization process easily causes the defective such as cell-cytotoxic reaction and affects its application clinically.Therefore in the urgent need to developing a kind of orthopaedics binding agent that can effectively bondingly have again outstanding biocompatibility.Utilize biomaterial to be put forward before an about century by Gluck the earliest as the idea of orthopaedics binding agent.Hedri has recorded and narrated a kind of being called " ossocol " orthopaedics biological adhesive (Christian H first in 1931, Ralf K, Dominique S, et al.Bone Adhesives in Trauma and Orthopedic Surgery.European Journal of Trauma2006; 32:141).Biological adhesive not only has good biocompatibility but also certain bonding strength is arranged, and is expected to satisfy orthopaedics to the requirement of binding agent.
Sodium alginate is a kind of biological polyoses that extracts from the Thallus Laminariae (Thallus Eckloniae) of Brown algae or Alga Sgrgassi Enerves.Alginate always as a kind of fabulous gel and thickening agent, is widely used for food and medical industry traditionally.Studies show that, sodium alginate can be used as the carrier of organizational project, and good biomedical character is arranged.The survival condition of cell in sodium alginate is close to interior state, be conducive to cellular matrix secretion and concentration and keep (Kavalkovich KW, Boynton RE, Murphy JM, et al.Chondrogenic differentiation of human mesenchymal stem cells within an alginate layer culture system[J] .In Vitro Cell Dev Biol Anim, 2002,38:457).Sodium alginate gel promotes the stem cell to osteoblast differentiation in addition, the adult rabbits chondrocyte can well be bred in the microenvironment that sodium alginate gel provides, special substrate (the Wang Yan of secretion cartilage, Chen Xiaoju, Wang Lan, Deng. the inducing bone mesenchymal differentiation of stem cells is osteoblast [J] on sodium alginate gel. Chinese biological engineering magazine, 2006,26 (9): 38).Chondroitin sulfate be a kind of in the cartilages such as mammal trachea, the extraction and acidic mucopolysaccharide, medically have antiinflammatory, accelerating wound healing, the clarification lipid improves body function of detoxification, the effects such as diuresis and analgesia.Sodium carboxymethyl cellulose, the native cellulose modification obtains modified cellulose, in food industry as emulsifying agent, thickening agent, in medical industry as the emulsion stabilizer of injection or binding agent and the film former of tablet medicament.
Sodium alginate, sodium carboxymethyl cellulose and chondroitin sulfate all are biological polyoses class materials, especially rear two kinds of raw materials medically use widely, but the relevant compound viscose glue of sodium alginate, sodium carboxymethyl cellulose and chondroitin sulfate is not reported both at home and abroad as skeletal agglutinant research.
Summary of the invention
The object of the invention is to not high for the existing bonding strength of existing adhesive of medical especially skeletal agglutinant, have in toxic and side effects, the human body problems such as degraded is difficult, provide a kind of improve on the forefathers basis have preparation easily, a kind of skeletal agglutinant based on sodium alginate take sodium alginate, sodium carboxymethyl cellulose and chondroitin sulfate as material of the advantages such as adhesion strength is high, curing performance is good, good biocompatibility and preparation method thereof.
Described skeletal agglutinant based on sodium alginate consisting of by mass percentage:
Sodium alginate: 1 ﹪～10 ﹪;
Sodium carboxymethyl cellulose: 1 ﹪～10 ﹪;
Chondroitin sulfate: 1 ﹪～5 ﹪;
Calsium supplement: 0 ﹪～50 ﹪;
Surplus is water.
Described calsium supplement can be selected from least a in calcium alginate, calcium phosphate, the calcium carbonate etc.
The concrete steps of the preparation method of described skeletal agglutinant based on sodium alginate are as follows:
Sodium alginate, sodium carboxymethyl cellulose, chondroitin sulfate and calsium supplement are added in the water, and mix homogeneously namely obtains the skeletal agglutinant based on sodium alginate, and the skeletal agglutinant based on sodium alginate of gained is gluey.
Compare with existing skeletal agglutinant, outstanding advantages of the present invention is to be with the stock of the biopolymer viscose glues such as sodium alginate, sodium carboxymethyl cellulose and chondroitin sulfate as skeletal agglutinant, have preparation section simple, stable in properties, with low cost.Also have simultaneously good biomechanics characteristic and biocompatibility, solidify rapidly, use and store the advantages such as convenient.Be equipped with in addition the calsium supplement that is beneficial to bone healing, both use the healing rate that is conducive to accelerate skeleton simultaneously.
The specific embodiment
Embodiment 1
The raw materials such as sodium alginate 4g, sodium carboxymethyl cellulose 1g, chondroitin sulfate 0.25g and calsium supplement calcium phosphate 1g are joined in the 92.75g water, mix homogeneously, namely obtain sodium alginate-sodium carboxymethyl cellulose-chondroitin sulfate composite skeletal agglutinant made, through test, dynamic viscosity reaches 1.81 * 10
6Mpas.
Embodiment 2
The raw materials such as sodium alginate 6g, sodium carboxymethyl cellulose 1.5g, chondroitin sulfate 0.75g and calsium supplement calcium alginate 1g are joined in the 90.75g water, mix homogeneously, namely obtain sodium alginate-sodium carboxymethyl cellulose-chondroitin sulfate composite skeletal agglutinant made, through test, dynamic viscosity reaches 1.92 * 10
6Mpas.
Embodiment 3
The raw materials such as sodium alginate 8g, sodium carboxymethyl cellulose 2g, chondroitin sulfate 0.5g and calsium supplement calcium carbonate 1.5g are joined in the 89g water, mix homogeneously, namely obtain sodium alginate-sodium carboxymethyl cellulose-chondroitin sulfate composite skeletal agglutinant made, through test, dynamic viscosity reaches 1.99 * 10
6Mpas.
Embodiment 4
The raw materials such as sodium alginate 5g, sodium carboxymethyl cellulose 1.5g, chondroitin sulfate 0.25g and calsium supplement calcium carbonate 1.5g are joined in the 91.75g water, mix homogeneously, namely obtain sodium alginate-sodium carboxymethyl cellulose-chondroitin sulfate composite skeletal agglutinant made, through test, dynamic viscosity reaches 1.89 * 10
6Mpas.
Embodiment 5
The raw materials such as sodium alginate 6g, sodium carboxymethyl cellulose 2.0g, chondroitin sulfate 0.75g and calsium supplement calcium phosphate 2g are joined in the 89.25g water, mix homogeneously, namely obtain sodium alginate-sodium carboxymethyl cellulose chondroitin sulfate composite skeletal agglutinant made, through test, dynamic viscosity reaches 1.87 * 10
6Mpas.
Embodiment 6
The raw materials such as sodium alginate 7g, sodium carboxymethyl cellulose 2.5g, chondroitin sulfate 0.5g and calsium supplement calcium alginate 1.5g are joined in the 88.5g water, mix homogeneously, namely obtain sodium alginate-sodium carboxymethyl cellulose-chondroitin sulfate composite skeletal agglutinant made, through test, dynamic viscosity reaches 1.96 * 10
6Mpas.
Embodiment 7
The raw materials such as sodium alginate 5g, sodium carboxymethyl cellulose 2.0g, chondroitin sulfate 0.75g and calsium supplement calcium phosphate 2g are joined in the 90.25g water, mix homogeneously, namely obtain sodium alginate-sodium carboxymethyl cellulose-chondroitin sulfate composite skeletal agglutinant made, through test, dynamic viscosity reaches 1.87 * 10
6Mpas.
Embodiment 8
The raw materials such as sodium alginate 6g, sodium carboxymethyl cellulose 5g, chondroitin sulfate 1g and calsium supplement calcium alginate 1g are joined in the 86g water, mix homogeneously, namely obtain sodium alginate-sodium carboxymethyl cellulose-chondroitin sulfate composite skeletal agglutinant made, through test, dynamic viscosity reaches 1.89 * 10
6Mpas.
Embodiment 9
The raw materials such as sodium alginate 7g, sodium carboxymethyl cellulose 4g, chondroitin sulfate 0.75g and calsium supplement calcium phosphate 2g are joined in the 86.25g water, mix homogeneously, namely obtain sodium alginate-sodium carboxymethyl cellulose-chondroitin sulfate composite skeletal agglutinant made, through test, dynamic viscosity reaches 1.91 * 10
6Mpas.
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Cited By (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103936881A (en) * | 2013-04-24 | 2014-07-23 | 新乡医学院 | Preparation methods of drug for promoting bone morphogenetie protein expression to accelerate fracture healing |
| CN104399114A (en) * | 2014-11-25 | 2015-03-11 | 苏州市贝克生物科技有限公司 | Adhesive for department of orthopaedics and preparation method of adhesive |
Citations (4)
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| US20020192263A1 (en) * | 1998-02-27 | 2002-12-19 | Merboth Barbara L. | Allograft bone composition having a gelatin binder |
| CN1966094A (en) * | 2006-10-25 | 2007-05-23 | 集美大学 | Compound bone cement of sodium alginate and Cyamopsis tetragonolobus gum and preparation method thereof |
| WO2008027864A2 (en) * | 2006-08-31 | 2008-03-06 | Warsaw Orthopedic, Inc. | Demineralized cancellous strip dbm graft |
| CN100435858C (en) * | 2006-10-23 | 2008-11-26 | 厦门大学 | The composite skeletal agglutinant made by sodium alginate-carboxyl methyl cellulose and its preparation method |
-
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Patent Citations (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20020192263A1 (en) * | 1998-02-27 | 2002-12-19 | Merboth Barbara L. | Allograft bone composition having a gelatin binder |
| WO2008027864A2 (en) * | 2006-08-31 | 2008-03-06 | Warsaw Orthopedic, Inc. | Demineralized cancellous strip dbm graft |
| CN100435858C (en) * | 2006-10-23 | 2008-11-26 | 厦门大学 | The composite skeletal agglutinant made by sodium alginate-carboxyl methyl cellulose and its preparation method |
| CN1966094A (en) * | 2006-10-25 | 2007-05-23 | 集美大学 | Compound bone cement of sodium alginate and Cyamopsis tetragonolobus gum and preparation method thereof |
Non-Patent Citations (1)
| Title |
|---|
| 蔡真真: "海藻混和胶粘接骨折的体外生物力学实验研究", 《中国优秀硕士论文WWW.CMFD.CNKI.NET》, 15 May 2011 (2011-05-15), pages 1 - 29 * |
Cited By (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN103936881A (en) * | 2013-04-24 | 2014-07-23 | 新乡医学院 | Preparation methods of drug for promoting bone morphogenetie protein expression to accelerate fracture healing |
| CN103936881B (en) * | 2013-04-24 | 2018-06-19 | 广州噢斯荣医药技术有限公司 | A kind of preparation method of the drug of promoting bone growing protein expression accelerating union of bone fracture |
| CN104399114A (en) * | 2014-11-25 | 2015-03-11 | 苏州市贝克生物科技有限公司 | Adhesive for department of orthopaedics and preparation method of adhesive |
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Application publication date: 20130417 |