CN103021249B - Heating carbon dioxide simulation abdominal cavity experimental box - Google Patents

Abstract

The invention relates to the technical field of medical experiment devices, in particular to a heating carbon dioxide simulation abdominal cavity experimental box which comprises a box body, a temperature and humidity sensor, a heating plate, a fan, a temperature and humidity controller, a universal bearing, a humidifying device and a camera. The head part, the chest and the back part of the experimental animal are closed, sewed and fixed in the containing channel, and the leaked CO is discharged through an exhaust hole arranged below the outer end of the containing channel₂The gas is prevented accumulating, and the oxygen hose that the holding passageway outer end top set up can provide oxygen for the laboratory animal, guarantees that the laboratory animal survives in the experimentation. The experiment operation instrument can help the experiment operator to extend into the laparoscope operation instrument through the Trocar of the universal bearing, and the experiment operation similar to the laparoscopic operation can be carried out on the experimental animal.

Description

Heated carbon dioxide simulated abdominal cavity test box

technical field

The invention relates to the technical field of medical experiment devices, in particular to a heating carbon dioxide simulated abdominal cavity experiment box.

Background technique

Since the introduction of the advanced technology of laparoscopy, the minimally invasive surgical technology represented by it has been widely used in various fields such as general surgery, obstetrics and gynecology, urology, chest surgery and plastic surgery. Minimally invasive surgery is developing at an unprecedented speed and is being It is recognized as one of the mainstream directions of surgical development in the 21st century. With the expansion of the scope of application of laparoscopic surgery, including the increased application in the diagnosis and treatment of malignant tumors, new problems have also emerged, that is, under the condition of using _CO2 as the gas peritoneum medium, tumor growth may be accelerated, easy to Problems such as recurrence and poking tumor implantation and metastasis. There have been many clinical reports of tumor cell proliferation and tumor metastasis at puncture holes after laparoscopy for abdominal malignant tumor CO ₂ pneumoperitoneum, making whether CO ₂ pneumoperitoneum is one of the factors that promote the proliferation and metastasis of malignant tumors has become an issue. The focus of the current study, moreover, what is the effect of heated _CO2 pneumoperitoneum on the proliferation and metastasis of malignant tumors? Is it as beneficial as intraperitoneal hyperthermic perfusion chemotherapy for patients with advanced tumors with abdominal metastases? The medical community is looking forward to a complete assessment of the feasibility and safety of laparoscopy in the diagnosis and treatment of malignant tumors. To this end, scholars have selected transplanted tumor animal models and in vitro cell culture techniques to investigate the effect of laparoscopic pneumoperitoneum on the proliferation and metastasis of malignant tumors, and to analyze its possible mechanism. Scholars have studied the effects of different CO ₂ pressures, pneumoperitoneum time, temperature and humidity on the proliferation and metastasis of malignant tumors and their specific mechanisms. However, due to ethical and moral factors, it is difficult to observe tumor patients throughout the process, and repeated invasive examinations cannot be performed, let alone invasive controlled experiments in humans. Therefore, with the help of in vitro animal models at home and abroad, by constructing immune mouse tumor models, and then constructing CO ₂ pneumoperitoneum or heated CO ₂ pneumoperitoneum models, to explore the effects of CO ₂ pneumoperitoneum or heated CO ₂ pneumoperitoneum on the proliferation and metastasis of malignant tumors influence and guide clinical practice.

Due to cost, model making and other reasons, small experimental animals, especially nude mice, are undoubtedly important experimental animals for tumor research. However, due to the current experimental equipment, experimental design or experimental process, due to the small body size and abdominal cavity of small experimental animals, it is impossible to perform laparoscopic surgery similar to the human abdominal cavity, or the experimental animals cannot survive during the experimental process, and it is impossible to obtain a similar human body. Intraperitoneal, ideal CO ₂ pneumoperitoneum or heated CO ₂ pneumoperitoneum experimental environment cannot be used for in vivo or live small animal experiments.

Contents of the invention

The purpose of the present invention is to further improve the deficiencies in the existing humid-heat _CO2 pneumoperitoneum experiment device, and design an abdominal cavity environment similar to that of the human abdominal cavity to carry out heated _CO2 laparoscopic surgery, and to apply it to animal experiments , to provide a living/in vivo animal experiment environment that simulates the heated CO ₂ abdominal cavity to ensure the survival of the experimental animals during the experiment. It is a heated carbon dioxide simulated abdominal cavity experimental box that is convenient for observing experiments and performing experimental operations similar to laparoscopic surgery on experimental animals.

A heating carbon dioxide simulated abdominal cavity experiment box, including a box body, a temperature and humidity sensor arranged in the box body, a heating plate, a fan and a temperature and humidity controller, and also includes a humidifying device and a camera located in the box body, on both sides of the box body An air outlet pipe and a through pipe are respectively provided, and the through pipe communicates with the air outlet of the humidification device. The box body is provided with an accommodating channel for accommodating experimental animals, and the inner end of the accommodating channel extends to the cavity in the box. The outer end of the accommodating passage extends to the outside of the box, the inner end of the accommodating passage is provided with a sealing film, the outer end of the accommodating passage is provided with an exhaust hole or an oxygen supply pipe, and the side of the accommodating passage is provided with a sealing film. The wall is mounted with universal bearings. the

Wherein, the box body includes a thermal insulation shell, a thermal insulation layer and a sealing layer arranged sequentially from outside to inside, the heating plate is evenly attached to the inner wall of the sealing layer, the fan is installed on the inner side of the heating plate, and the temperature and humidity control The device is located in the insulation shell, and a temperature and humidity sensor is installed inside the sealing layer.

Wherein, the humidifying device is installed inside the heat preservation shell.

Wherein, the sealing layer is provided with a transparent cover plate which can be opened and closed.

Wherein, the accommodating channel is cylindrical.

Wherein, the outlet pipe is equipped with a control valve.

Wherein, the box body is equipped with a universal tube, the inner end of the universal tube protrudes into the box body, and the camera is fixedly installed on the inner end of the universal tube.

Wherein, an ultraviolet germicidal lamp is installed inside the sealing layer.

Wherein, the inner wall of the sealing layer is provided with two supports, and the two ends of the ultraviolet germicidal lamp are respectively fixed on the two supports.

The beneficial effects of the present invention are: the present invention provides a heating carbon dioxide simulated abdominal cavity experiment box. During the experiment, the body of the animal is allowed to pass through the middle part of the sealing film, and the fur on the chest and back of the animal is sewed with the sealing film, and then the animal is Fix it in the holding channel, with the head of the animal facing outwards and the body facing the inside of the box. Finally, the sealing film is sealed and connected with the inner end of the holding channel to complete the preparation for the simulation experiment. The exhaust hole set under the outer end of the storage channel discharges the leaked CO ₂ gas to prevent accumulation. The oxygen tube set above the outer end of the storage channel can provide oxygen for experimental animals. ₂ Laparoscopic surgery operates in the same or similar abdominal cavity environment, and is applied to animal experiments to provide a living/in vivo animal experimental environment that simulates heated CO ₂ abdominal cavity, ensuring the survival of experimental animals during the experiment, and the experimental effect is good. The casing of the present invention is equipped with a camera, controls the shooting position and direction of the camera by operating the universal bearing externally, observes and records information by the camera, and helps the experimental operator extend into the laparoscopic operation device through the Trocar fixed on the universal bearing. Experimental animals are subjected to experimental operations similar to laparoscopic surgery, which is very practical.

Description of drawings

Fig. 1 is a schematic diagram of the three-dimensional structure of the present invention.

Fig. 2 is a left view structural schematic diagram of the present invention.

Fig. 3 is a schematic cross-sectional structure diagram of the section A-A in Fig. 2 .

Detailed ways

In order to facilitate the understanding of those skilled in the art, the present invention will be further described below in conjunction with the embodiments and accompanying drawings, and the contents mentioned in the embodiments are not intended to limit the present invention.

As shown in Figures 1 to 3, a heating carbon dioxide simulated abdominal cavity test box includes a box body 1, a temperature and humidity sensor 2 arranged in the box body 1, a heating plate 3, a fan 4 and a temperature and humidity controller 5, and also includes There is a humidifying device 6 and a camera 18 located in the casing 1, and an air outlet pipe 14 and a through pipe 15 are respectively arranged on both sides of the casing 1, and the through pipe 15 communicates with the air outlet of the humidifying device 6, and the casing 1 is provided with There is an accommodating channel 7 for accommodating experimental animals, the inner end of the accommodating channel 7 extends to the cavity in the box body 1, and the outer end of the accommodating channel 7 extends to the outside of the box body 1, accommodating The inner end of the passage 7 is provided with a sealing film 8 , the outer end of the accommodating passage 7 is provided with an exhaust hole 9 , and the side wall of the box 1 is provided with a universal bearing 21 . the

Taking the mouse simulation experiment as an example, in the actual experiment process, let the body of the mouse pass through the middle of the sealing film 8, and suture the fur on the chest and back of the mouse with the sealing film 8, and then fix the mouse in the accommodating channel 7 , the head of the mouse is facing outwards, and the body is facing the inside of the box body 1. Finally, the sealing film 8 is sealed and connected with the inner end of the accommodating channel 7 to complete the preparation for the simulation experiment. The exhaust hole set under the outer end of the storage channel discharges the leaked CO ₂ gas to prevent accumulation, and the oxygen tube set above _the outer end of the storage channel can provide oxygen for the mice. Laparoscopic surgery operates in the same or similar abdominal cavity environment and is applied to mouse experiments to provide a living/in vivo mouse experimental environment that simulates heated CO ₂ abdominal cavity to ensure the survival of mice during the experiment, and the experimental effect is better.

The trocar (Trocar) enters the box body 1 through the universal bearing 21. Due to the setting of the universal bearing 21, the trocar (Trocar) can move freely in the box body 1, and the trocar and the universal bearing The contact portion of 21 has a good sealing effect, which is convenient for experimenters to perform laparoscopic surgery on the abdominal cavity of the mouse in the box 1. A preferred embodiment is that there are two universal bearings 21 , which is convenient for the left and right hands to operate the two trocars at the same time.

The casing 1 of the present invention is equipped with a camera 18, and the shooting position and direction of the camera 18 are controlled by operating the universal bearing 17 externally. Operate instruments and perform experimental operations similar to laparoscopic surgery on experimental animals. , Strong practicability.

The box body 1 of this embodiment includes a thermal insulation shell 10, a thermal insulation layer 11 and a sealing layer 12 arranged sequentially from outside to inside, the heating plate 3 is evenly attached to the inner wall of the sealing layer 12, and the fan 4 is installed on the heating Inside the plate 3, the temperature and humidity controller 5 is located in the heat preservation shell 10, and the temperature and humidity sensor 2 is installed inside the sealing layer 12.

When in use, the external _CO2 gas enters the interior of the humidifier 6 through the preset air inlet on the thermal insulation shell 10 for humidification treatment, and the humidified _CO2 gas then enters the sealing layer 12 through the gas outlet of the humidifier 6 , under the action of the heating plate 3 and the fan 4 in the sealing layer 12, the CO ₂ gas in the box 1 is uniformly mixed, and reaches a state of constant temperature and humidity. Simultaneously, the temperature and humidity of the CO in the casing 1 are monitored in real time by the temperature and humidity sensor ₂ , and then the temperature and humidity in the casing 1 are effectively regulated by the temperature and humidity controller 5 on the insulation shell 10, so that The CO ₂ temperature and humidity in the box 1 reaches a preset level, and the uniformity of temperature and humidity in the sealing layer 12 is effectively improved, and the experimental environment provided by the humid-heat CO ₂ pneumoperitoneum test device is more practical and reliable.

The humidifying device 6 of this embodiment is installed inside the heat preservation shell 10 . Moreover, the air inlet of the humidifying device 6 is connected to the preset air inlet on the heat preservation shell 10 through a pipe, which greatly improves the structure compactness of the humid heat CO ₂ pneumoperitoneum experiment device, and the humidification effect is better.

The sealing layer 12 in this embodiment is equipped with a transparent cover 13 that can be opened and closed. Through the transparent cover plate 13, the experimental conditions in the sealing layer 12 can be observed in real time during use, so as to facilitate timely understanding of the progress of the experiment.

The accommodating channel 7 in this embodiment is cylindrical, so as to facilitate placing the mouse inside the accommodating channel 7 , and at the same time, the connection between the sealing film 8 and the cylindrical accommodating channel 7 is more airtight.

The gas outlet pipe 14 of this embodiment is equipped with a control valve 16, so as to adjust the flow rate of the gas in the humid-heat CO ₂ insufflation test device for convenient control.

The box body 1 of this embodiment is equipped with a universal tube 17, the inner end of the universal tube 17 extends into the inside of the box body 1, and the camera 18 is fixedly installed on the inner end of the universal tube 17 . The experimenter controls the shooting position and direction of the camera 18 through the external operation of the universal bearing 17, and the camera observes and records information to help the experiment operator extend into the laparoscope to operate the instrument through the Trocar pierced through the universal tube 17, and perform similar operations on the experimental animals. Laparoscopic surgery-like experimental operation.

In this embodiment, an ultraviolet germicidal lamp 19 is installed inside the sealing layer 12 . Specifically, the inner wall of the sealing layer 12 is provided with two supports 20 , and the two ends of the ultraviolet germicidal lamp 19 are respectively fixed to the two supports 20 . The ultraviolet germicidal lamp 19 is fixed on the inner sidewall of the sealing layer 12 by two supports 20, and this device can effectively sterilize and disinfect the _CO gas in the casing 1 and other experimental objects when needed.

The above-mentioned embodiments are preferred implementation solutions of the present invention. In addition, the present invention can also be realized in other ways, and any obvious replacements are within the protection scope of the present invention without departing from the concept of the present invention.

Claims (9)

1. Heating carbon dioxide simulated abdominal cavity experiment box, including box body (1), temperature and humidity controller (5), temperature and humidity sensor (2) installed in the box body (1), heating plate (3) and fan (4) , which is characterized in that: it also includes a humidifying device (6) and a camera (18) located in the box (1), and the two sides of the box (1) are respectively provided with an air outlet pipe (14) and a through pipe (15), The through pipe (15) communicates with the air outlet of the humidifying device (6), and the box (1) is provided with an accommodating channel (7) for accommodating experimental animals, and the inner end of the accommodating channel (7) extends to The cavity in the box (1), the outer end of the accommodating channel (7) extends to the outside of the box (1), and the inner end of the accommodating channel (7) is equipped with a sealing film (8), The outer end of the accommodation passage (7) is provided with an exhaust hole (9) and an oxygen supply pipe, and the side wall of the box (1) is provided with a universal bearing (21). 2. The heated carbon dioxide simulated abdominal cavity experiment box according to claim 1, characterized in that: the box body (1) includes an insulation shell (10), an insulation layer (11) and a sealing layer (12) arranged sequentially from outside to inside ), the heating plate (3) is evenly attached to the inner wall of the sealing layer (12), the fan (4) is installed on the inner side of the heating plate (3), and the temperature and humidity controller (5) is located in the heat preservation shell ( 10), a temperature and humidity sensor (2) is installed inside the sealing layer (12). 3 . The heated carbon dioxide simulated abdominal cavity experiment box according to claim 2 , characterized in that: the humidifying device ( 6 ) is installed inside the heat preservation shell ( 10 ). 4. The heated carbon dioxide simulated abdominal cavity experiment box according to claim 2, characterized in that: the sealing layer (12) is equipped with a transparent cover plate (13) that can be opened and closed. 5. The heated carbon dioxide simulated abdominal cavity test box according to claim 1, characterized in that: the accommodating channel (7) is cylindrical. 6 . The heating carbon dioxide simulated abdominal cavity experiment box according to claim 1 , characterized in that: the outlet pipe ( 14 ) is equipped with a control valve ( 16 ). 7. The heating carbon dioxide simulated abdominal cavity experiment box according to claim 1, characterized in that: said box body (1) is equipped with a universal tube (17), and the inner end of the universal tube (17) extends into Inside the box (1), the camera (18) is fixedly installed at the inner end of the universal tube (17). 8. The heating carbon dioxide simulated abdominal cavity test box according to claim 1, characterized in that: an ultraviolet germicidal lamp (19) is installed inside the sealing layer (12). 9. The heating carbon dioxide simulated abdominal cavity experiment box according to claim 8, characterized in that: the inner wall of the sealing layer (12) is provided with two supports (20), and the two supports (20) of the ultraviolet germicidal lamp (19) The ends are respectively fixed on the two supports (20).