CN103008195A - Sealing adhesive method for PAGE slabs in electrophoresis tank - Google Patents

Abstract

The invention relates to a sealing adhesive method for PAGE slabs in an electrophoresis tank, and belongs to the technical field of biology. As the current sealing adhesive technology for the PAGE slabs adopts manual coating adhesive from top to bottom, the requirement on the operation technique is high, the phenomena of un-uniform sealing adhesive and imprecise caulking joint are often generated, adhesive leakage is caused, and a test is failed. The sealing adhesive method adopts a mode of automatic flowing from bottom to top to coat adhesive, so that the operation is simple, convenient and fast, the agar powder dosage is less, and the sealing adhesive effect is good.

Description

A kind of sealing method of PAGE rubber plate in electrophoresis tank

technical field

The invention discloses a method for sealing a PAGE rubber plate in an electrophoresis tank, and belongs to the field of biotechnology. More specifically, it is a new method for rapid sealing of PAGE gel plates in a PAGE gel electrophoresis tank.

Background technique

PAGE gel electrophoresis technology is a conventional technique in molecular biology, mainly used to separate DNA, RNA, proteins, isozymes, etc. When separating isoenzymes, it is necessary to use agar solution to plug and seal the gap between the glass plate and the tank wall of the electrophoresis tank to form a glue chamber. This work is called glue sealing. The quality requirements of the sealing work are very high. If the sealing is not strict, there will be leakage after adding the subsequent glue, which is called glue leakage, which will make the follow-up work impossible. The characteristic of the existing PAGE plate sealing method is that the glue is applied manually, and the operation is carried out from top to bottom. The specific method is to use a pipette to draw the agar solution, and add it dropwise along the gap between the tank wall and the glass plate from top to bottom. In this method, because the agar powder liquid is manually squeezed out of the pipette tip, it is difficult to perform continuous and uniform operation throughout the sealing process, and the agar powder liquid is easy to solidify, which often causes the agar powder liquid to solidify into lumps in the middle of the side. , leaving a gap that cannot be detected by the human naked eye, causing glue leakage in the follow-up operation, resulting in rework and a lot of labor and materials.

Contents of the invention

The purpose of the invention of a method for sealing PAGE rubber plates in an electrophoresis tank is to improve the method and flow of the sealing operation, so that the sealing work can not only ensure that the glue does not leak, but also is simple, fast and efficient, and saves labor and materials.

The present invention is achieved like this:

1. A method for sealing PAGE rubber plates in an electrophoresis tank includes filling the gap between the glass plate and the glue chamber with agar solution, and it is characterized in that the sealing method also includes first down, then up, and rapid gel flow Require.

2. The first bottom and then top one refers to sealing the horizontal gap at the bottom of the glue chamber first, and then sealing the longitudinal gap of the glue chamber; the second refers to sealing the four longitudinal seams of the glue chamber from bottom to top Plastic closures.

The fast gel flow one means to accumulate the agar solution at the corner of the bottom first, and then quickly adjust the placement angle of the electrophoresis tank, so that the agar solution flows rapidly in one direction along the horizontal gap at the bottom, where the solution goes, immediately Enter the gap, cool and condense, and seal the gap. In this way, continue to operate until the horizontal gaps around the bottom are all closed. The second is to pile up the agar solution at the corner of the bottom first, and then quickly adjust the placement angle of the electrophoresis tank, so that the agar solution flows rapidly from bottom to top along the longitudinal gap of the gel chamber. Wherever the solution goes, it immediately enters the gap. Cooling condenses and seals the gap. In this way, continue to operate until the longitudinal gaps of the glue chamber are completely closed.

During the flow of the agar solution, if the solution cools and condenses and no longer flows, that is, refill the agar solution at this point, and adjust the angle of the electrophoresis tank to make it continue to flow in the same direction until the gap is completely closed.

Beneficial effects of the present invention: a method for sealing PAGE rubber plates in an electrophoresis tank is suitable for PAGE operations that require sealing with agar powder, and has the following advantages: first, it is easy to operate. This method abandons the traditional way of manually dripping and spreading glue, and instead concentrates the agar solution at the bottom corner of the glue chamber and then makes it flow quickly along the gap of the glue chamber to seal the upper and lower gaps of the glue chamber, which is more convenient to operate; , the bottom-up gluing operation can make the whole gluing process be carried out under the close watch of the eyes, and can observe all the details of the agar solution flowing along the gap and entering the gap, and adjust the posture of the electrophoresis tank at any time, so that the sealing The speed of the glue is fast and the effect is good; third, the amount of agar powder used is small, and the sealing effect is good. In the process of rapid flow along the gap, the method spreads the glue thinly and evenly, does not form agar powder bumps, and has a good sealing effect. Fourth, save time. After filling the agar solution, quickly tilt the electrophoresis tank to make it flow along the gap. The operation is quite fast, and it does not need to be sucked and added dropwise like the traditional method, which can save time.

Detailed ways:

The present invention will be further described below in conjunction with specific embodiments.

Example 1: Quick sealing of double-plate sandwich type vertical electrophoresis tank DYCZ-30CPAGE plastic plate (length*height specification: 105×185mm)

We use a method of sealing the PAGE gel plate in the electrophoresis tank in which the gel is first lowered and then upper, and the gel is quickly flowed. The first is down first and then up: first seal the bottom of the glue chamber, and then seal the longitudinal gaps of the glue chamber; the second is to seal the four longitudinal seams of the glue chamber from bottom to top. The specific method is to pile up the agar solution at the corner of the bottom first, and then quickly adjust the angle of the electrophoresis tank so that the agar solution flows quickly in one direction along the horizontal gap at the bottom. Wherever the solution goes, it immediately enters the gap. Cooling condenses and seals the gap. In this way, continue to operate until the horizontal gaps around the bottom are all closed. Then pile up the agar solution at the corner of the bottom, quickly adjust the placement angle of the electrophoresis tank, so that the agar solution flows quickly along the longitudinal gap of the gel chamber, where the solution goes, it immediately enters the gap, cools and condenses, and the gap closed. In this way, continue to operate until the longitudinal gaps of the glue chamber are completely closed.

During the flow of the agar solution, if it is found that the solution cools and condenses and no longer flows, then refill the agar solution at this point, adjust the angle of the electrophoresis tank to make it continue to flow in the same direction until the gap is completely closed.

After passing the above steps, check the water filling of the glue chamber, and there is no water leakage at the bottom and side.

Example 2: Quick sealing of veneer sandwich vertical groove-DYCZ-28A rubber sheet (length*height specification: 125×100mm)

Follow the same steps and methods as in Example 1 to quickly seal the veneer sandwich vertical groove-DYCZ-28A rubber sheet. After the completion, check the water injection of the glue room, and there is no water leakage at the bottom and side.

Claims (8)

1. The sealing method of PAGE rubber plate in a kind of electrophoresis tank comprises filling the gap between glass plate and glue room with agar solution, it is characterized in that, described sealing method also has first down and then up, fast flow glue Require. 2 . The sealing method according to claim 1 , characterized in that, first down and then up refers to sealing the horizontal gap at the bottom of the glue chamber first, and then sealing the longitudinal gap of the glue chamber. 3 . The sealing method according to claim 1 , characterized in that, the first down and then up refers to sealing the four longitudinal seams of the glue chamber from bottom to top. 4. The sealing method according to claim 1, characterized in that, said rapid flow of glue means that the agar solution is accumulated at the corner of the bottom first, and then the placement angle of the electrophoresis tank is quickly adjusted so that the agar solution runs along the bottom. The horizontal gaps of the liquid flow rapidly in one direction, and where the solution goes, it immediately enters the gaps, cools and condenses, and seals the gaps. 5. In this way, continue to operate until the horizontal gaps around the bottom are all closed. 6. The sealing method according to claim 1, characterized in that, said rapid flow of glue refers to first accumulating agar solution at the corner of the bottom, and then quickly adjusting the placement angle of the electrophoresis tank so that the agar solution runs along the glue The longitudinal gap of the chamber flows rapidly from bottom to top, where the solution goes, it immediately enters the gap, cools and condenses, and seals the gap. 7. In this way, continue to operate until the longitudinal gap of the glue chamber is completely closed. 8. The sealing method according to claim 4 and claim 5, characterized in that, during the flow of the agar solution, if the solution cools and condenses and no longer flows, that is, refill the agar solution at this point to adjust the electrophoresis After adjusting the groove angle, it continues to flow in the same direction until the gap is completely closed.